Processing method of cornu cervi pantotrichum and prepared traditional Chinese medicine composition

Abstract

The invention provides a pilose antler processing method and a prepared traditional Chinese medicine composition, and belongs to the technical field of medicine processing. The invention provides a processing method of cornu cervi pantotrichum, which comprises the following steps of: freezing harvested cornu cervi pantotrichum at low temperature for a period of time, naturally thawing, removing cornu cervi pantotrichum fur, performing low-temperature treatment again, slicing and drying to prepare a dry cornu cervi pantotrichum blood slice. According to the processing method disclosed by the invention, active growth factor components of the hairy antler can be greatly reserved, hemoglobin is not lost, water-soluble small molecular proteins are effectively reserved, biological active substances in the hairy antler are not denatured, and the activity of the biological active substances is reserved. The processed cornu cervi pantotrichum, lucid ganoderma, rhizoma polygonati, American ginseng and fructus lycii are mixed according to a certain proportion and then subjected to ultrasonic water extraction, a water extracting solution and a concentrated solution are subjected to fluid extract, then the oral liquid is prepared, and the content of total saponins in the oral liquid is rich.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine processing, and particularly relates to a method for processing pilose antler and a traditional Chinese medicine composition prepared thereby. Background Art

[0002] Pilose antler is a precious traditional Chinese medicine, taken from the unossified young antlers of sika deer (Cervus nippon Temminck) or red deer (C. elaphus L), so it can be regarded as a natural growth factor library. Ancient records show that pilose antler is sweet in taste and warm in nature, mainly treating metrorrhagia and leukorrhagia, aversion to cold and fever, epilepsy, strengthening qi and will, promoting tooth growth without aging, warming the kidney and strengthening yang, generating essence and blood, strengthening tendons and replenishing marrow. It is mainly used to treat consumptive fatigue and emaciation, dizziness due to blood deficiency, soreness and weakness of the waist and knees, impotence and premature ejaculation, non-healing injuries for a long time, brain strengthening, intelligence improvement, blood generation, body strengthening, bone setting, improving sexual function, and promoting wound healing. The formula of the preparation is mainly based on pilose antler. Modern pharmacological research shows that pilose antler has various pharmacological effects, such as cardiovascular and nervous system effects; effects on sugar, lipid, and protein metabolism; immunomodulatory, anti-inflammatory, anti-tumor, brain strengthening, anti-aging and other pharmacological effects.

[0003] Before use, pilose antler also needs to be processed. Especially, the common method is to remove the skin and hair by using a fire roasting method, that is, using an ordinary spray gun to burn off the fluff on the pilose antler, then putting the pilose antler into boiling water for blanching, first softening it and then solidifying the protein, and using a scraper to remove the epidermis of the pilose antler. When the pilose antler is put into boiling water for blanching, the protein in it will deteriorate due to high temperature, lose its activity, and thus lose its biological efficacy. Therefore, it is necessary to create a new processing technology. Summary of the Invention

[0004] The present invention provides a method for processing pilose antler and a traditional Chinese medicine composition prepared thereby. By the method for processing pilose antler of the present invention, the activity of the biologically active substances in the pilose antler can be retained.

[0005] The present invention provides a method for processing pilose antler, comprising the following steps: first freezing the harvested pilose antler in an environment of -25 to -10 °C, taking it out for the first natural thawing and then removing the fur of the pilose antler, then freezing the pilose antler without fur in an environment of -25 to -10 °C, taking it out, slicing and drying to obtain pilose antler blood slices.

[0006] In a preferred embodiment of the present invention, the pilose antler includes red deer pilose antler.

[0007] In a preferred embodiment of the present invention, the time for the first freezing is 36 to 48 h.

[0008] In a preferred embodiment of the present invention, the time for the first natural thawing is 4 h.

[0009] In a preferred embodiment of the present invention, the time for the second freezing is 18 to 24 h.

[0010] In a preferred embodiment of the present invention, the drying includes freeze-drying.

[0011] The present invention also provides pilose antler blood slices obtained by the above processing method.

[0012] The present invention also provides a traditional Chinese medicine composition with biological activity, and the raw materials include the above-mentioned pilose antler blood slices, Ganoderma lucidum, Polygonatum sibiricum, American ginseng, and wolfberry fruits;

[0013] The mass ratio of Ganoderma lucidum: American ginseng: wolfberry fruits: Polygonatum sibiricum: pilose antler blood slices is 1:1:1.2: (1.5 - 1.6): (0.17 - 0.34).

[0014] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine composition, including the following steps: mixing each raw material with water and then performing ultrasonic extraction. When performing the ultrasonic extraction, the temperature is 40 - 45 °C, the ultrasonic frequency of the ultrasonic wave is 20 kHz, and the extraction time is 2 - 2.5 h.

[0015] The present invention also provides an oral liquid containing the active ingredients of the above-mentioned traditional Chinese medicine composition.

[0016] Beneficial effects: The present invention provides a processing method of pilose antler. First, the harvested pilose antler is subjected to low-temperature treatment for a period of time and then naturally thawed. After removing the pilose antler fur, it is subjected to low-temperature treatment again, sliced, and dried to obtain dried pilose antler blood slices.

[0017] The method of low-temperature freezing, thawing, and re-freezing of pilose antler in the present invention is used to process pilose antler, which can greatly retain the active growth factor components of pilose antler of Cervus elaphus xanthopygus, without loss of hemoglobin, effectively retain water-soluble small-molecule proteins, and without denaturing the bioactive substances in pilose antler, and retain the activity of bioactive substances. In the examples of the present invention, the water extract of dried pilose antler blood slices of Cervus elaphus xanthopygus was verified, and the content of hemoglobin could reach 3 g / L. The present invention also mixes the processed pilose antler with Ganoderma lucidum, Polygonatum sibiricum, American ginseng, and wolfberry fruits in a certain proportion, performs ultrasonic water extraction, concentrates the water extraction solution to obtain a fluid extract, and then prepares an oral liquid. The content of total saponins (calculated as ginsenoside Re) in the oral liquid can reach 0.56 g / 100 mL. Specific embodiments

[0018] The present invention provides a processing method of pilose antler, including the following steps: subjecting the harvested pilose antler to a first freezing in an environment of -25 to -10 °C, taking it out for the first natural thawing, removing the pilose antler fur, subjecting the pilose antler without fur to a second freezing in an environment of -25 to -10 °C, taking it out, slicing, and drying to obtain pilose antler blood slices.

[0019] The present invention does not impose any special restrictions on the source of the pilose antler. For example, in the embodiment, the pilose antler is harvested from a wapiti. The present invention does not impose any special restrictions on the harvesting method, and conventional methods in the art can be used for harvesting.

[0020] The present invention first performs a first freezing treatment on the harvested pilose antler. The temperature of the first freezing is -25°C, and the time is 36 to 48 hours.

[0021] After taking out the pilose antler after the first freezing treatment, the present invention performs natural thawing. The natural thawing is carried out at room temperature, and the room temperature condition is 20 to 25°C. The time of the first natural thawing in the present invention can be 4 hours. In the present invention, a variable temperature method of freezing and sublimation is used to remove the fur. By using the change of temperature, the fur of the pilose antler is separated from the underlying connective tissue, which is convenient for subsequent physical fur removal.

[0022] The present invention processes the pilose antler after the first thawing. The processing includes removing the fur of the pilose antler. For example, in the embodiment, the fur of the pilose antler is shaved off with a knife, and pilose antler blood oozes out on the surface of the freshly shaved pilose antler.

[0023] Then, the skinned pilose antler is frozen again. The temperature of the freezing is -25°C, and the time of the second freezing is 18 to 24 hours. The present invention slices the pilose antler after the second freezing into pilose antler blood slices about 2 mm thick. The present invention freeze-dries the pilose antler slices to obtain dry pilose antler blood slices. The present invention does not impose any special restrictions on the slicing method. For example, in the embodiment, a horseback-type electric pilose antler slicing machine is used for slicing. In one embodiment, the parameters of the freeze-drying in the present invention are set as follows: pre-cooling temperature -18°C to -20°C for 24 hours, cold trap temperature -26°C, vacuum degree -0.08 to -0.1 Pa, freezing time 24 to 48 hours, sublimation temperature 40 to 45°C, and sublimation time 20 to 24 hours.

[0024] After obtaining the dry pilose antler blood slices, the present invention can also perform pulverization, such as pulverizing into small particles that can pass through a 6-mm mesh sieve.

[0025] The present invention also provides pilose antler blood slices obtained by the above processing method.

[0026] The dry pilose antler blood slices in the present invention can greatly retain the active growth factor components of the pilose antler of wapiti, without loss of hemoglobin, effectively retain water-soluble small molecule proteins, and do not denature the bioactive substances in the pilose antler, and retain the activity of the bioactive substances. In Example 1 of the present invention, the water extract of the dry pilose antler blood slices of wapiti was verified, and the content of hemoglobin could reach 3 g / L.

[0027] The present invention also provides a traditional Chinese medicine composition with a bioactive effect. The raw materials include the above-mentioned pilose antler blood slices, Ganoderma lucidum, Polygonatum sibiricum, American ginseng, and wolfberry fruit;

[0028] The mass ratio of Ganoderma lucidum: American ginseng: wolfberry fruit: polygonatum sibiricum: deer antler blood slices is 1:1:1.2:(1.5 - 1.6):(0.17 - 0.34).

[0029] The formula of the materials described in the present invention can enable a large number of bioactive substances to be retained in the product, such as growth factors sensitive to temperature, such as IGF-1, TGF-β, etc.

[0030] The present invention also provides a preparation method of the above traditional Chinese medicine composition, including the following steps: mixing each raw material with water and then performing ultrasonic extraction. When performing the ultrasonic extraction, the temperature is 40 - 45°C, the ultrasonic frequency of the ultrasonic wave is 20 kHz, and the extraction time is 2 - 2.5 h.

[0031] In the present invention, each material is mixed with water and then subjected to ultrasonic water extraction. The mass ratio of the mixed materials to water can be 1:8. For example, the mixed liquid is placed in a vertical tank microwave sterilizer as shown in Chinese Patent CN111671940B for extraction. Before the formal extraction, a pretreatment process is also included, that is, mixing is carried out in the tank. The rotation speed during the mixing is 30 - 40 rpm, the temperature is controlled at 40 - 50°C, and the time is 15 min. At this time, the ultrasonic frequency of the ultrasonic wave is controlled at 20 kHz and 2500 W.

[0032] After the pretreatment described in the present invention, ultrasonic extraction is carried out. The temperature in the tank is 40 - 45°C, the ultrasonic power is 2000 - 3000 W / unit × 4 units, the pressure is -0.05 - -0.08 Pa, the rotation speed of the stirrer is 25 rpm, and the extraction is carried out for 2 - 2.5 hours.

[0033] After the ultrasonic extraction described in the present invention, preliminary purification is carried out, that is, the supernatant is allowed to stand under vacuum conditions. The vacuum degree of the vacuum conditions is -0.8 - -0.9 Pa, and the standing time is 12 h.

[0034] The present invention concentrates the purified clear liquid to prepare an extract. The operating temperature is 45 - 55°C, the vacuum degree of the vacuum extraction is -0.7 - -0.8 Pa, and it can be concentrated until the water content is 10 - 15%. In an embodiment of the present invention, extraction at 42°C belongs to a mild temperature. The fat-soluble substances after extraction are completely dissolved in the extraction solution. When concentrated under the condition of low temperature 55°C, no decomposition of components such as fat-soluble substances (growth factors) is seen in the concentrated extraction solution. However, after the extraction solution material is flash sterilized at 115°C / 30 minutes by filling a glass control bottle, there are visible cloud-like floating substances in the bottle. When the bottle is gently held by hand, these cloud-like floating substances immediately disappear in the oral liquid in the bottle.

[0035] After obtaining the extract, the present invention further includes drying the extract by spray drying to prepare a dry paste powder. The parameters of freeze-drying are as follows: precooling temperature -18°C, cold trap temperature -22°C, vacuum degree -0.1 Pa, freezing time 22 h, sublimation temperature 18°C.

[0036] The present invention also provides an oral liquid containing the active ingredients of the above traditional Chinese medicine composition.

[0037] The oral liquid of the present invention further includes excipients such as xylitol, potassium sorbate, stevioside and water. In one embodiment of the present invention, 1000 g of American ginseng, 1000 g of Ganoderma lucidum, 1200 g of wolfberries, 170 g of pilose antler and 1600 g of polygonatum sibiricum are selected for extraction and concentration of the extract. The extraction and concentration methods are the same as above and will not be elaborated here. The present invention selects 120 g of xylitol, 4 g of potassium sorbate and 2 g of stevioside for mixing, and uses purified water as a solvent for constant volume to 10000 mL, making a total of 1000 pieces, 10 mL / piece.

[0038] In order to further illustrate the present invention, the following is a detailed description of a pilose antler processing method and the prepared traditional Chinese medicine composition provided by the present invention in combination with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0039] Example 1

[0040] 1. First, freeze the harvested pilose antler of Cervus elaphus at -25°C for 48 h, and take it out for natural thawing for 4 h;

[0041] 2. Use a knife to shave off the fur of the pilose antler of Cervus elaphus. There is pilose antler blood oozing out on the surface of the freshly shaved pilose antler of Cervus elaphus.

[0042] 3. Then perform secondary freezing for 24 h. After the second freezing is completed, take it out and cut it into pilose antler blood slices about 2 mm thick with a horseback type electric pilose antler slicing machine, and then freeze-dry it to obtain dry pilose antler blood slices, and crush them into small particles through a 6 mm mesh sieve.

[0043] Then prepare the oral liquid, and the prepared oral liquid is named Kunzhilin Brand Pilose Antler Ganoderma Lucidum Polygonatum Sibiricum Oral Liquid:

[0044] Ingredients: 1000 g of American ginseng, 1000 g of Ganoderma lucidum, 1200 g of wolfberries, 170 g of pilose antler particles and 1600 g of polygonatum sibiricum; 120 g of xylitol, 4 g of potassium sorbate and 2 g of stevioside, and use purified water as a solvent for constant volume to 10000 mL, making a total of 1000 pieces, 10 mL / piece.

[0045] Preparation plan: Process description

[0046] (1) Pretreatment

[0047] Pick out the impurities from the Ganoderma lucidum, American ginseng, wolfberry fruit, pilose antler, and polygonatum odoratum Chinese herbal pieces that meet the requirements, weigh them, and set aside. After passing the inspection, remove the outer packaging of xylitol, stevioside, and potassium sorbate and enter the clean area for standby.

[0048] (2) Extraction, filtration, and concentration

[0049] Extraction and filtration: Soak the Ganoderma lucidum, American ginseng, wolfberry fruit, pilose antler, and polygonatum odoratum for 2 hours, place them in the extraction tank, mix them in the tank, with a rotation speed of 30 - 40 rpm, control the temperature at 40 - 50 °C, for 15 minutes, and control the ultrasonic frequency at 20 kHz and 2500 W; perform ultrasonic extraction after pretreatment, with the temperature in the tank at 40 - 45 °C, ultrasonic power of 2000 - 3000 W / unit × 4 units, pressure of -0.05 - -0.08 Pa, stirrer rotation speed of 25 rpm, and extract for 2 - 2.5 hours;

[0050] Vacuum concentration: The operating temperature is 45 - 55 °C, the vacuum degree is -0.7 - -0.8 Pa, concentrate until the water content is 10 - 15%, and the relative density is 1.05 - 1.10 (60 °C) to obtain the concentrated solution for standby.

[0051] Drying paste powder: The pre-cooling temperature is -18 °C, the cold trap temperature is -22 °C, the vacuum degree is -0.1 Pa, the freezing time is 22 hours, and the sublimation temperature is 18 °C.

[0052] (3) Solution preparation

[0053] Standing: Dissolve the dried paste powder and let it stand, then take the supernatant. Filter the supernatant through a 100-mesh sieve to obtain Solution A.

[0054] Weighing: Weigh xylitol, stevioside, and potassium sorbate respectively according to the formula amount for standby;

[0055] Dissolution and sieving: Add appropriate purified water to the formula amount of xylitol and stevioside, stir to dissolve, and pass through a 100-mesh sieve to obtain Solution B for standby. Add appropriate purified water to the formula amount of potassium sorbate, stir to dissolve, and pass through a 100-mesh sieve to obtain Solution C for standby;

[0056] Volume fixing and mixing: Place Solution A, Solution B, and Solution C in the solution preparation tank, add purified water to fix the volume to the formula amount, mix for 20 minutes, filter to obtain the original oral liquid solution for standby.

[0057] (4) Washing the bottles and caps

[0058] Wash the oral liquid bottles successively with drinking water and purified water, dry them for standby; wash the oral liquid bottle caps successively with drinking water and purified water, soak them in 75% ethanol, and air dry for standby.

[0059] (5) Filling and capping

[0060] Place the qualified oral liquid stock solution on an oral liquid filling and capping machine for filling, with a filling volume of 10 ml per vial, cap the vial, and check the sealing. The inner packaging material is a sodium calcium glass controlled oral liquid bottle, and the standard complies with the requirements of sodium calcium glass controlled oral liquid bottle YBB00032004.

[0061] (6) Sterilization

[0062] Place the filled product in a sterilizer for sterilization. Use heat-pressure sterilization at a temperature of 115 °C and a pressure of 68.6 KPa for 30 minutes.

[0063] (7) Lamp inspection, packaging, labeling, finished product inspection, and warehousing

[0064] Inspect the sterilized oral liquid under a lamp inspection lamp, and reject unqualified products such as insufficient filling volume, loose sealing, breakage, and foreign objects. The lamp inspection qualified products are packed into packaging boxes according to the specifications, 10 vials per box, and labeled. Randomly select a batch of samples and conduct finished product inspection according to the methods and requirements described in the quality standard of this product. After passing the inspection, store them in the warehouse.

[0065] (8) Environmental health and cleanliness requirements for production

[0066] The production environment and management should meet the requirements of Good Manufacturing Practice for Health Foods. During production, processes such as liquid preparation (standing, weighing, dissolving, sieving, volume fixing, mixing), washing bottles and caps with purified water, filling, and capping are all operated under the conditions of a 100,000-class production clean area, and others are carried out in the general production area.

[0067] Randomly sample and entrust the Nanjing Product Quality Supervision and Inspection Institute and Nanjing Institute of Quality Development and Advanced Technology Application for testing. The test reports are shown in Table 1 and Table 2:

[0068] Table 1 Test Report

[0069]

[0070]

[0071] Table 2 Indicative ingredient indicators

[0072]

[0073] The oral liquid of the present invention contains a relatively large amount of hemoglobin. Hemoglobin is insoluble in water but can form a colloidal solution with water. Proteins usually have hydrophilic and hydrophobic regions, and under the action of negative pressure ultrasonic cavitation, they can interact with water molecules. The molecular part of hemoglobin can bind to water molecules, causing it to disperse in water and form a colloidal solution. A colloidal solution is a state between a solution and a suspension. Hemoglobin molecules are dispersed in water but not completely dissolved. This solution appears transparent or translucent but is not in a truly dissolved state. From the observation of the appearance of this prepared oral liquid, it can be seen with the naked eye that the colloidal solution is suspended in the liquid in the bottle.

[0074] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for processing pilose antler, characterized in that: The following steps are involved: The harvested velvet antlers are first frozen in an environment of -25 to -10°C, taken out for first natural thawing, and then the velvet antler fur is removed. The velvet antlers with fur removed are second frozen in an environment of -25 to -10°C, taken out, sliced ​​and dried to obtain velvet antler blood slices.

2. The preparation method according to claim 1, characterized in that: The velvet antler includes red deer antler.

3. The preparation method according to claim 1, characterized in that: The first freezing time is 36 to 48 hours.

4. The processing method according to claim 1 or 3, characterized in that: The first natural thawing time is 4 hours.

5. The preparation method according to claim 1, characterized in that: The second freezing time is 18 to 24 hours.

6. The preparation method according to claim 1, characterized in that: The drying includes freeze drying.

7. Deer antler blood slices obtained by the processing method according to any one of claims 1 to 6.

8. A Chinese medicine composition with biological activity, characterized in that: The raw materials include the pilose antler blood slices, ganoderma lucidum, polygonatum sibiricum, American ginseng and wolfberry as described in claim 7; The mass ratio of the ganoderma lucidum: American ginseng: wolfberry: polygonatum: pilose antler blood slices is 1:1:1.2:(1.5-1.6):(0.17-0.34).

9. The method for preparing the Chinese medicine composition according to claim 8, characterized in that: The following steps are involved: After mixing the raw materials with water, ultrasonic extraction is performed. During the ultrasonic extraction, the temperature is 40-45° C., the ultrasonic frequency is 20 kHz, and the extraction time is 2-2.5 hours.

10. An oral liquid comprising the effective ingredients of the Chinese medicine composition according to claim 8.

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