Zanthoxylum bungeanum leaf extract as well as preparation method and application thereof

Abstract

The invention discloses a zanthoxylum bungeanum leaf extract and a preparation method and application thereof.The preparation method includes the steps that zanthoxylum bungeanum leaves are cleaned, dried and then smashed, and zanthoxylum bungeanum leaf powder is obtained; condensing and extracting: taking the bunge pricklyash leaf powder, adding a solvent, heating, condensing and refluxing for a certain time, carrying out suction filtration after condensing and refluxing, collecting filtrate, repeatedly extracting for 2-5 times, adding the same volume of solvent each time, and combining the filtrate to obtain an extract; performing rotary evaporation: performing rotary evaporation on the extracting solution to obtain a concentrated solution; and drying: drying the concentrated solution, and grinding the dried concentrated solution into powder to obtain the bunge pricklyash leaf extract. The zanthoxylum bungeanum leaf extract prepared by the preparation method disclosed by the invention has an inhibiting effect on listeria monocytogenes, staphylococcus aureus, streptococcus agalactiae and bacillus cereus, provides a new way for preparing bacteriostatic products such as bacteriostatic drugs, bacteriostatic cleaning supplies and bacteriostatic feed additives, and is beneficial to development and utilization of zanthoxylum bungeanum leaf resources; the use value of bunge pricklyash leaves is improved, and resource waste is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of phytochemistry. More specifically, the present invention relates to an extract of Zanthoxylum bungeanum leaves, a preparation method thereof, and an application thereof. Background Art

[0002] The fruits of Zanthoxylum bungeanum maxim are relatively small. During the collection process of Zanthoxylum bungeanum raw materials, the fruits of Zanthoxylum bungeanum are often picked together with the branches and leaves of Zanthoxylum bungeanum. The main raw material for making Zanthoxylum bungeanum in the food industry is the pericarp of Zanthoxylum bungeanum. A large number of by-products such as Zanthoxylum bungeanum leaves, Zanthoxylum bungeanum seeds, and Zanthoxylum bungeanum bark are generated during the production process of Zanthoxylum bungeanum. These by-products are often discarded or burned, ultimately causing environmental pollution and waste of resources. Zanthoxylum bungeanum leaves contain rich nutritional components such as proteins, carbohydrates, phenolic compounds, vitamins, etc. Its chemical components mainly include alkaloids, amides, lignans, coumarins, triterpenes, sterols, hydrocarbons, and flavonoids, etc., and can be used in the fields of feed, food, medicine, cosmetics, pesticides, detergents, etc. However, the current development and utilization of Zanthoxylum bungeanum leaves are insufficient, resulting in waste of resources. Therefore, it is of great significance to develop and utilize Zanthoxylum bungeanum leaf resources and improve their use value in different fields. Summary of the Invention

[0003] An object of the present invention is to solve at least the above problems and / or deficiencies, and provide at least the advantages described hereinafter.

[0004] In order to achieve these objects and other advantages of the present invention, an extract of Zanthoxylum bungeanum leaves is provided. The preparation method of the extract of Zanthoxylum bungeanum leaves includes the following steps:

[0005] Step 1: Wash the Zanthoxylum bungeanum leaves, dry them at 60 - 70°C, and then crush them to obtain Zanthoxylum bungeanum leaf powder;

[0006] Step 2: Condensation extraction: Take the Zanthoxylum bungeanum leaf powder, add a solvent, heat and reflux for a certain time, perform suction filtration after condensation reflux, collect the filtrate, repeat the extraction 2 - 5 times, add the same volume of solvent each time, combine the filtrates to obtain an extract;

[0007] Step 3: Rotary evaporation: Rotarily evaporate the extract to obtain a concentrated solution;

[0008] Step 4: Drying: Dry the concentrated solution and grind it into a powder to obtain an extract of Zanthoxylum bungeanum leaves.

[0009] Preferably, in Step 1, the drying temperature is 60 - 70°C; and it is crushed to 6 - 8 meshes.

[0010] Preferably, in the second step, the solvent is distilled water or a 30 wt% to 70 wt% ethanol solution; the mass-volume ratio of the prickly ash leaf powder to the solvent is 1 g: 15 - 30 mL.

[0011] Preferably, in the second step, the condensation reflux temperature is 60 - 100 °C and the time is 0.5 - 2 h.

[0012] Preferably, in the third step, the specific method of rotary evaporation is as follows: Add one-third to one-half of the volume of the extract to the rotary flask, set the initial temperature to 40 - 50 °C, and after the condensation reflux is stable, increase the temperature at a rate of 1 - 3 °C / min to 55 - 65 °C, and rotary evaporate the extract to 1 / 4 - 1 / 8 of the original volume.

[0013] Preferably, in the fourth step, the drying temperature is 30 - 50 °C and it is ground to 50 - 100 mesh.

[0014] An application of the prickly ash leaf extract as described above, wherein the prickly ash leaf extract is used to prepare an antibacterial product.

[0015] An application of the prickly ash leaf extract as described above, wherein the prickly ash leaf extract is used to prepare a composite with antibacterial efficacy.

[0016] Preferably, the preparation method of the composite with antibacterial efficacy includes the following steps:

[0017] S1. Weigh 8 - 12 parts of prickly ash leaf extract, 2 - 4 parts of tamarix chinensis extract, 1 - 3 parts of dandelion extract, and 1 - 2 parts of shikimic acid by weight, add them to a 10 - 50 wt% ethanol solution, with a material-liquid ratio of 1 g: 8 - 15 mL, carry out ultrasonic treatment at 10 - 30 kHz and 200 - 400 W for 10 - 30 min, and vacuum dry at 30 - 50 °C to obtain a mixture;

[0018] S2. Treat the mixture with low-temperature plasma for 2 - 5 min to obtain a composite with antibacterial efficacy.

[0019] Preferably, the preparation method of the tamarix chinensis extract is as follows: Wash the tamarix chinensis branches, dry them at 60 - 80 °C and then crush them to 30 - 100 mesh, add 10 - 20 times the mass of a 50 - 70 wt% ethanol solution, extract at 60 - 90 °C for 3 times, 1 hour each time, filter by suction to collect the filtrate, combine the 3 filtrates, and concentrate and dry to obtain the tamarix chinensis extract.

[0020] Preferably, the preparation method of the dandelion extract is as follows: Crush the dried whole dandelion herb to 30 - 100 mesh, add 10 - 30 times the mass of distilled water, extract at 80 - 100 °C for 3 times, 1 hour each time, filter by suction to collect the filtrate, combine the 3 filtrates, and concentrate and dry to obtain the dandelion extract.

[0021] Preferably, the voltage of the low-temperature plasma treatment is 10 - 50 kV, the frequency is 50 - 150 Hz, the working gas is a mixed gas of oxygen and nitrogen, and the volume ratio of oxygen to nitrogen is 1:1.

[0022] A composite with antibacterial efficacy prepared by the preparation method as described above.

[0023] An application of a composite with antibacterial efficacy as described above, wherein the composite with antibacterial efficacy is used for preparing antibacterial products.

[0024] Preferably, the zanthoxylum bungeanum leaf extract is used for preparing antibacterial products that inhibit listeria, staphylococcus aureus, streptococcus agalactiae, and bacillus cereus.

[0025] Preferably, the composite with antibacterial efficacy is used for preparing antibacterial products that inhibit listeria, staphylococcus aureus, streptococcus agalactiae, or bacillus cereus.

[0026] Preferably, the antibacterial products include antibacterial drugs, antibacterial cleaning products, or antibacterial feed additives.

[0027] The present invention has at least the following beneficial effects: The present invention prepares a zanthoxylum bungeanum leaf extract, which has inhibitory effects on listeria, staphylococcus aureus, streptococcus agalactiae, and bacillus cereus, provides a new way for preparing antibacterial products such as antibacterial drugs, antibacterial cleaning products, and antibacterial feed additives, and helps to develop and utilize the zanthoxylum bungeanum leaf resources, improve the use value of the zanthoxylum bungeanum leaf, and reduce resource waste. In addition, the present invention combines the zanthoxylum bungeanum leaf extract, tamarix chinensis extract, taraxacum extract, and shikimic acid, and each substance has a synergistic effect, which can effectively improve the antibacterial activity. Among them, the surface of the mixture is modified by low-temperature plasma, and the prepared composite has excellent antibacterial ability.

[0028] Other advantages, objectives, and features of the present invention will be partially reflected by the following description, and partially will be understood by those skilled in the art through the research and practice of the present invention. Description of the Drawings

[0029] Figure 1 The antibacterial experiment results of the zanthoxylum bungeanum leaf extract prepared in Example 1 diluted to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%) with distilled water against staphylococcus aureus;

[0030] Figure 2The antibacterial test results of the Zanthoxylum bungeanum leaf extract prepared in Example 2 diluted to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%) with distilled water against Staphylococcus aureus;

[0031] Figure 3 The antibacterial test results of the Zanthoxylum bungeanum leaf extract prepared in Example 3 diluted to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%) with distilled water against Staphylococcus aureus;

[0032] Figure 4 The antibacterial test results of the Zanthoxylum bungeanum leaf extract prepared in Example 4 diluted to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%) with distilled water against Staphylococcus aureus;

[0033] Figure 5 The antibacterial test results of the Zanthoxylum bungeanum leaf extracts prepared in Examples 1 - 4 diluted to 25 wt% concentration with distilled water against Staphylococcus aureus; a is Example 1, b is Example 2, c is Example 4, d is Example 3;

[0034] Figure 6 The antibacterial test results of the Zanthoxylum bungeanum leaf extracts prepared in Examples 1 - 4 diluted to 25 wt% concentration with distilled water against Listeria monocytogenes;

[0035] Figure 7 The antibacterial test results of the Zanthoxylum bungeanum leaf extracts prepared in Examples 1 - 4 diluted to 25 wt% concentration with distilled water against Streptococcus agalactiae;

[0036] Figure 8 The antibacterial test results of the Zanthoxylum bungeanum leaf extracts prepared in Examples 1 - 4 diluted to 25 wt% concentration with distilled water against Bacillus cereus. Detailed implementation mode

[0037] The following further describes the present invention in detail with reference to the accompanying drawings, so that those skilled in the art can implement it according to the description in the specification.

[0038] It should be understood that the terms such as "having", "comprising", and "including" used herein do not exclude the presence or addition of one or more other elements or their combinations.

[0039] The test materials used in the following examples include:

[0040] Extract preparation: round-bottom flask (1 L), serpentine condenser, water bath, cold water hose, iron stand, Buchner funnel, qualitative filter paper, suction flask, circulating water vacuum pump, rotary evaporator and supporting rotary flask (2 L), oven, evaporating dish;

[0041] Antibacterial test: 200-μL pipette, LB medium, spreader, and plate puncher.

[0042] Example 1

[0043] A method for preparing a Zanthoxylum bungeanum leaf extract, comprising the following steps:

[0044] Step 1: Wash the Zanthoxylum bungeanum leaves, dry them at 65 °C, and then crush them to 7 mesh to obtain Zanthoxylum bungeanum leaf powder.

[0045] Step 2: Condensation extraction: Condense and reflux the Zanthoxylum bungeanum leaf powder with 30 wt% ethanol solution in a water bath at 70 °C for 1 hour, with a material-liquid ratio of 1 g:25 mL. After 1 hour of condensation and reflux, perform suction filtration, collect the filtrate, repeat the extraction three times, each time adding an equal volume of 30 wt% ethanol solution, and combine the three filtrates to obtain an extract.

[0046] Step 3: Rotary evaporation: Add one-third of its volume of the extract to a rotary flask (2 L) and perform rotary evaporation. The initial temperature is 45 °C. After the condensation and reflux are stable, increase the temperature at a rate of 1 °C / min to 65 °C, and stop when the volume is evaporated to about 100 mL to obtain a concentrated solution.

[0047] Step 4: Drying: Pour the concentrated solution into an evaporating dish, dry it at 40 °C, and grind it to 60 mesh to obtain the Zanthoxylum bungeanum leaf extract.

[0048] Example 2

[0049] In this example, 50 wt% ethanol solution is used for condensation extraction, and the remaining steps are the same as those in Example 1.

[0050] Example 3

[0051] In this example, 70 wt% ethanol solution is used for condensation extraction, and the remaining steps are the same as those in Example 1.

[0052] Example 4

[0053] In this example, distilled water is used for condensation extraction, and the remaining steps are the same as those in Example 1.

[0054] Example 5

[0055] A method for preparing a complex with antibacterial efficacy, comprising the following steps:

[0056] S1. Weigh 10 parts of the Zanthoxylum bungeanum leaf extract prepared in Example 3, 3 parts of Tamarix ramosissima extract, 2 parts of Taraxacum mongolicum extract, and 1 part of shikimic acid by weight, add them to 30 wt% ethanol solution, with a material-liquid ratio of 1 g:10 mL, perform ultrasonic treatment at 20 kHz and 300 W for 20 min, and then dry it under vacuum at 40 °C to obtain a mixture.

[0057] S2. Treat the mixture with low-temperature plasma to obtain a composite with antibacterial efficacy; wherein, the frequency of the low-temperature plasma treatment is 100 Hz, the voltage is 40 kV, the treatment time is 3 minutes, and the working gas is a mixed gas of oxygen and nitrogen (volume ratio 1:1).

[0058] The preparation method of the tamarisk extract is as follows: Wash the tamarisk branches, dry them at 70 °C and then crush them to 50 mesh, add them to 15 times the mass of 60 wt% ethanol solution, extract 3 times at 70 °C, 1 hour each time, filter by suction to collect the filtrate, combine the 3 filtrates, concentrate and dry to obtain the tamarisk extract.

[0059] The preparation method of the dandelion extract is as follows: Crush the dried whole dandelion herb to 50 mesh, add 20 times the mass of distilled water, extract 3 times at 100 °C, 1 hour each time, filter by suction to collect the filtrate, combine the 3 filtrates, concentrate and dry to obtain the dandelion extract.

[0060] Example 6

[0061] In this example, by weight, 8 parts of the zanthoxylum leaf extract prepared in Example 3, 4 parts of the tamarisk extract, 3 parts of the dandelion extract, and 1 part of shikimic acid; the remaining steps are the same as in Example 5.

[0062] Example 7

[0063] In this example, by weight, 12 parts of the zanthoxylum leaf extract prepared in Example 3, 2 parts of the tamarisk extract, 1 part of the dandelion extract, and 2 parts of shikimic acid; the remaining steps are the same as in Example 5.

[0064] Comparative Example 1

[0065] In this comparative example, no low-temperature plasma treatment is carried out, and the remaining steps are the same as in Example 5.

[0066] Comparative Example 2

[0067] In this comparative example, no tamarisk extract is added, and the remaining steps are the same as in Example 5.

[0068] Comparative Example 3

[0069] In this comparative example, no dandelion extract is added, and the remaining steps are the same as in Example 5.

[0070] Comparative Example 4

[0071] In this comparative example, no shikimic acid is added, and the remaining steps are the same as in Example 5.

[0072] Antibacterial test:

[0073] (1) Agar punching antibacterial method

[0074] 1) Preparation of culture medium and broth:

[0075] LB medium: Weigh 40 g of LB nutrient agar (Haibo Biotechnology Co., Ltd., Qingdao High-tech Industrial Park), add the LB nutrient agar to distilled water and heat it to 60 °C until completely dissolved. Make up the volume to 1000 mL with distilled water. After volume adjustment, dispense it. Put an appropriate amount of the culture medium into 250 mL conical flasks respectively, seal them with cotton plugs and kraft paper, and sterilize them by autoclaving at 121 °C for 15 min. The petri dishes are also subjected to autoclaving treatment. When pouring the antibacterial LB medium, pay attention to the thickness of the culture medium, generally 20 mL is appropriate, and for the ordinary LB medium, 15 mL is appropriate.

[0076] LB broth: Weigh 25 g of LB broth (Haibo Biotechnology Co., Ltd., Qingdao High-tech Industrial Park), add the LB broth to distilled water and heat it to 60 °C until completely dissolved. Make up the volume to 1000 mL with distilled water. After volume adjustment, dispense it into 250 mL conical flasks, 20 mL per bottle is appropriate, seal them with cotton plugs and kraft paper, and sterilize them by autoclaving at 121 °C for 15 min.

[0077] 2) Activation of bacterial strains and preparation of bacterial suspensions: Activate Staphylococcus aureus (or other bacteria) in the LB medium for 8 h, check for contamination by miscellaneous bacteria, spread it in a laminar flow hood, and culture it in the LB medium for 3 generations. Select the bacterial strain with the best viability, inoculate the activated test bacteria into the LB broth, culture for 8 h, and prepare the obtained test bacteria into a bacterial suspension with an OD 600 of 1.9.

[0078] 3) Coating of bacterial suspension and punching: Dilute the bacterial suspension with PBS to 10 -3 gradient. Use a 200 μL pipette to suck 100 μL of the diluted bacterial suspension and add it to the antibacterial LB medium, spread it evenly with a spreading rod, and then use a sterilized round punch to punch round holes with appropriate distance and size on the antibacterial LB medium for sample addition. The above steps are all carried out in a laminar flow hood.

[0079] 4) Antibacterial experiment: On the laminar flow hood, dilute the Zanthoxylum bungeanum leaf extracts prepared in Examples 1-4 with distilled water to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%, 25 wt%). Then use a 200 μL pipette to suck 150 μL and add it to the round holes of the antibacterial LB medium. Place the petri dishes in a 4 °C refrigerator for 8 h. Wait until the diluted extract solution is completely absorbed by the culture medium, and then put it into a 37 °C constant temperature incubator for culture and observation. After 24 h, check the size of the antibacterial zone and measure its diameter (cm). Obtain the Zanthoxylum bungeanum leaf extract by the method with the best inhibitory effect, and observe the inhibitory effect of the Zanthoxylum bungeanum leaf extract on different bacterial strains.

[0080] (2) Data analysis

[0081] The experimental data was statistically analyzed using SPSS 17.0 software. The experimental results were expressed as mean ± standard error, and P < 0.05 was considered significantly different.

[0082] The experimental results are as Figures 1-8 shown. In the figure, the prickly ash leaf extract obtained in Example 1 is labeled as H1 or 1, the prickly ash leaf extract obtained in Example 2 is labeled as H2 or 2, the prickly ash leaf extract obtained in Example 3 is labeled as H3 or 3, and the prickly ash leaf extract obtained in Example 4 is labeled as H4 or 4.

[0083] The prickly ash leaf extracts obtained in Examples 1 - 4 were respectively diluted with distilled water to different concentrations (0.25 wt%, 0.5 wt%, 1 wt%, 2.5 wt%, 5 wt%, 25 wt%) for antibacterial tests against Staphylococcus aureus. The results are shown in Table 1, Figures 1-5 as shown. It can be seen that among the dilution concentrations adopted in this experiment, when the sample dilution concentration is 25 wt%, the antibacterial effect is the best.

[0084] Table 1 Antibacterial effects of prickly ash leaf extracts against Staphylococcus aureus at different dilution concentrations

[0085]

[0086] The prickly ash leaf extracts obtained in Examples 1 - 4 were respectively diluted with distilled water to a concentration of 25 wt% for antibacterial tests against different Gram - positive bacteria (Listeria monocytogenes, Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus). The results are shown in Table 2, Figures 5-8 as shown. It can be seen that the prickly ash leaf extract has inhibitory effects on Listeria monocytogenes ( Figure 6 ), Staphylococcus aureus ( Figure 5 ), Streptococcus agalactiae ( Figure 7 ) and Bacillus cereus ( Figure 8 ), and the inhibitory effect on spherical bacteria such as Streptococcus agalactiae and Staphylococcus aureus is the most obvious. The inhibitory effect is the best when the ethanol concentration used for sample extraction is 50 wt% and 70 wt%, and there is no significant difference between the two.

[0087] Table 2 Antibacterial effects of prickly ash leaf extracts at a 25 wt% dilution concentration against different Gram - positive bacteria

[0088]

[0089]

[0090] The present invention respectively extracts Zanthoxylum bungeanum leaf extracts with 30wt% ethanol, 50wt% ethanol, 70wt% ethanol and distilled water, and dilutes them with distilled water to different concentrations for antibacterial tests. The test results show that among the dilution concentrations selected in this test, when the dilution concentration is 25wt%, the antibacterial effect of the Zanthoxylum bungeanum leaf extract is better, and it has obvious inhibitory effects on Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus and Listeria, and the inhibitory effects decrease in the above order, and the inhibitory effect on the bacterial species with coccus cell morphology is the best; among them, the antibacterial effects of the Zanthoxylum bungeanum leaf extracts extracted with 50wt% ethanol (Example 2) and 70wt% ethanol (Example 3) are better, but there is no significant difference. In summary, the Zanthoxylum bungeanum leaf extract prepared by the present invention can be used to prepare antibacterial products, such as antibacterial drugs, antibacterial cleaning products, antibacterial feed additives, etc., and the antibacterial products can be used to inhibit Listeria, Staphylococcus aureus, Streptococcus agalactiae or Bacillus cereus.

[0091] The complexes prepared in Examples 5-7 and Comparative Examples 1-4 were respectively diluted to a concentration of 25wt% with distilled water, and antibacterial tests were carried out on different Gram-positive bacteria (Listeria, Staphylococcus aureus, Streptococcus agalactiae, Bacillus cereus). The results are shown in Table 3. It can be seen that the present invention uses Zanthoxylum bungeanum leaf extract, Tamarix ramosissima extract, Taraxacum mongolicum extract and shikimic acid for compounding, and then uses low-temperature plasma treatment to prepare a complex with excellent antibacterial ability, and its inhibitory ability against Listeria, Staphylococcus aureus, Streptococcus agalactiae or Bacillus cereus is better than that of Examples 1-4 and Comparative Examples 1-4. The complex with antibacterial efficacy prepared by the present invention can be used to prepare antibacterial products, such as antibacterial drugs, antibacterial cleaning products, antibacterial feed additives, etc., and the antibacterial products can be used to inhibit Listeria, Staphylococcus aureus, Streptococcus agalactiae or Bacillus cereus.

[0092] Table 3 Antibacterial effects of the complex with antibacterial efficacy on different Gram-positive bacteria at a 25wt% dilution concentration

[0093]

[0094]

[0095] Although the embodiments of the present invention have been disclosed as above, it is not limited to the applications listed in the specification and embodiments. It can be fully applied to various fields suitable for the present invention. For those skilled in the art, additional modifications can be easily made. Therefore, without departing from the general concept defined by the claims and the equivalent scope, the present invention is not limited to the specific details and the examples shown and described here.

Claims

1. A Zanthoxylum bungeanum leaf extract, characterized in that The preparation method of the Zanthoxylum bungeanum leaf extract comprises the following steps: Step 1: Wash the Zanthoxylum bungeanum leaves, dry them and crush them to obtain Zanthoxylum bungeanum leaf powder; Step 2, condensation extraction: take Zanthoxylum bungeanum leaf powder, add solvent, heat condensation reflux for a certain period of time, filter after condensation reflux, collect the filtrate, repeat the extraction 2 to 5 times, add an equal volume of solvent each time, combine the filtrate, and obtain an extract; Step 3, rotary evaporation: rotary evaporating the extract to obtain a concentrated solution; Step 4, drying: drying the concentrated solution and grinding it into powder to obtain the Zanthoxylum bungeanum leaf extract.

2. The Zanthoxylum bungeanum leaf extract according to claim 1, characterized in that: In the step 1, the drying temperature is 60-70° C. and the powder is crushed to 6-8 mesh.

3. The Zanthoxylum bungeanum leaf extract according to claim 1, characterized in that: In the step 2, the solvent is distilled water or a 30wt% to 70wt% ethanol solution; the mass volume ratio of the Zanthoxylum bungeanum leaf powder to the solvent is 1g:15 to 30mL.

4. The Zanthoxylum bungeanum leaf extract according to claim 1, characterized in that: In the step 2, the condensation reflux temperature is 60-100° C. and the time is 0.5-2 h.

5. The Zanthoxylum bungeanum leaf extract according to claim 1, characterized in that: In the step three, the specific method of rotary evaporation is: add one-third to one-half of the volume of the extract into the rotating bottle, set the initial temperature to 40-50°C, and after the condensation reflux is stable, increase the temperature to 55-65°C at a heating rate of 1-3°C / min, and rotary evaporate the extract to 1 / 4-1 / 8 of the original volume.

6. The Zanthoxylum bungeanum leaf extract according to claim 1, characterized in that: In the step 4, the drying temperature is 30-50° C. and the powder is ground into 50-100 meshes.

7. A use of the Zanthoxylum bungeanum leaf extract according to any one of claims 1 to 6, characterized in that: The Zanthoxylum bungeanum leaf extract is used for preparing antibacterial products.

8. The use according to claim 7, characterized in that The Zanthoxylum bungeanum leaf extract is used to prepare a compound with antibacterial efficacy, and the compound with antibacterial efficacy is used to prepare an antibacterial product; wherein the preparation method of the compound with antibacterial efficacy comprises the following steps: S1. Weigh 8-12 parts of Zanthoxylum leaf extract, 2-4 parts of red willow extract, 1-3 parts of dandelion extract and 1-2 parts of shikimic acid by weight, add them to 10-50wt% ethanol solution, with a solid-liquid ratio of 1g:8-15mL, ultrasonically treat at 10-30kHz, 200-400W for 10-30min, and vacuum dry at 30-50°C to obtain a mixture; S2. Treat the mixture with low-temperature plasma for 2 to 5 minutes to obtain a composite with antibacterial effect.

9. The use according to claim 8, characterized in that The Zanthoxylum bungeanum leaf extract or the compound with antibacterial effect is used to prepare an antibacterial product for inhibiting Listeria, Staphylococcus aureus, Streptococcus agalactiae and Bacillus cereus.

10. The use according to claim 9, characterized in that The antibacterial products include antibacterial drugs, antibacterial cleaning products or antibacterial feed additives.

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