Preparation method of medical antibacterial sponge

Abstract

The invention discloses a preparation method of medical antibacterial sponge, and belongs to the technical field of antibacterial dressings. Silver-loaded polyurethane nanofibers are prepared through electrostatic spinning; doping a natural antibacterial compound and magnolol into the silver-loaded fluorinated polyurethane nanofiber membrane to obtain an antibacterial base material; and carrying out sterilization treatment on the antibacterial base material, and carrying out combined packaging. According to the preparation method, silver ions are used as a precursor through an electrostatic spinning technology, in the process of preparing the polyurethane nanofibers, silver nanoparticles are loaded and embedded into the surfaces of the polyurethane nanofibers, and slow-release particles of honokiol can be adsorbed by coating the surfaces of the polyurethane nanofibers with dopamine coatings; the continuous antibacterial effect of the polyurethane nanofiber membrane layer can be prolonged through the slow-release honokiol.

Description

Technical Field

[0001] The present invention belongs to the technical field of antibacterial dressings, and particularly relates to a preparation method of a medical antibacterial sponge. Background Art

[0002] An antibacterial dressing is a medical dressing with antibacterial function, mainly used to cover and protect wounds, prevent infection and promote wound healing. External ion antibacterial dressings usually contain antibacterial components, and are breathable, soft, and have good water absorption. They can release antibacterial components to inhibit and eliminate pathogens, and keep the wound dry and clean.

[0003] The Chinese invention patent with the authorization announcement number CN105400214B discloses a method for preparing hemostatic cotton using hyaluronic acid crosslinked gelatin, belonging to the field of biomedical materials. In the present invention, a novel aldehyde hyaluronic acid crosslinking agent is first prepared, and then the aldehyde hyaluronic acid crosslinking agent is mixed with gelatin for foaming, freeze-dried, and sterilized to obtain gelatin hemostatic cotton. In the present invention, sodium periodate, a strong oxidant, is used to oxidize the carbon-carbon bond of the cis-diol in hyaluronic acid to make it into an aldehyde group that can react with amino groups. The aldehyde group-modified hyaluronic acid is used as a macromolecular crosslinking agent to react with gelatin with amino functional groups, so that gelatin forms a crosslinked network structure, thereby enhancing the mechanical properties and endowing the gelatin sponge with functionality. Gelatin has good biocompatibility and has hemostatic and antibacterial functions by itself. Using it as a sponge substrate and aldehyde hyaluronic acid as a crosslinking agent, the prepared hemostatic cotton has good mechanical properties;

[0004] However, in actual use, when the antibacterial sponge absorbs a large amount of liquid, if it is not replaced or kept dry in time, a moist environment will be formed on its surface. Such an environment is conducive to the growth and reproduction of bacteria, especially those bacteria with strong adaptability to the moist environment, such as Staphylococcus aureus and Pseudomonas aeruginosa. The reproduction of bacteria will increase the risk of wound infection. Once bacteria reproduce on the contact surface of the antibacterial sponge, they may spread to the wound surface through direct contact or the flow of liquid, affecting the quality of wound healing. Even if the wound heals eventually, bacterial infection may cause adverse reactions such as scar hyperplasia and tissue adhesion, and there is room for improvement. Summary of the Invention

[0005] The purpose of the present invention is to propose a preparation method of a medical antibacterial sponge to solve the problem that the antibacterial sponge is prone to bacterial reproduction due to the wetness of the contact surface after fully absorbing liquid.

[0006] In order to achieve the above purpose, the present invention adopts the following technical scheme:

[0007] A preparation method of a medical antibacterial sponge includes the following steps:

[0008] Prepare silver-loaded polyurethane nanofibers by electrospinning;

[0009] Incorporate honokiol, a natural antibacterial compound, into a silver-loaded fluorinated polyurethane nanofiber membrane to obtain an antibacterial substrate;

[0010] Perform a disinfection treatment on the antibacterial substrate and carry out combined packaging.

[0011] As a further description of the above technical solution:

[0012] The preparation method of the sustained-release particles of honokiol includes:

[0013] Prepare the internal aqueous phase: Dissolve honokiol in water to form the internal aqueous phase;

[0014] Prepare the primary emulsion: Dissolve poly(lactic-co-glycolic acid) copolymer in the organic solvent dichloromethane, then add the internal aqueous phase to the poly(lactic-co-glycolic acid) copolymer organic solution, and after adding it to a vacuum stirring kettle, evacuate and stir for 2 - 5 min to form the primary emulsion;

[0015] Prepare the multiple emulsion: Transfer the primary emulsion to an aqueous solution containing an emulsifier in the external aqueous phase and form a multiple emulsion by further stirring;

[0016] Spray drying: Dry the multiple emulsion through a spray drying device to remove the organic solvent, and solidify the poly(lactic-co-glycolic acid) copolymer during the spraying process to form microspheres;

[0017] Post-treatment: Centrifuge and filter to collect the microspheres, wash them with deionized water to remove the uncoated honokiol and residual organic solvents, and finally freeze-dry to obtain solid sustained-release microspheres.

[0018] As a further description of the above technical solution:

[0019] The rotation speed of the vacuum stirring kettle is 1500 - 1800 r / min.

[0020] As a further description of the above technical solution:

[0021] The preparation method of the silver-loaded fluorinated polyurethane nanofiber membrane includes:

[0022] Prepare the polyurethane solution: Dissolve polyurethane in dimethylformamide;

[0023] Add the silver precursor: Add silver nitrate as the silver precursor to the polyurethane solution;

[0024] Add the reducing agent: Add tannic acid as the reducing agent to promote the reduction of silver ions to form silver nanoparticles;

[0025] Mix and stir: Thoroughly mix and stir the above solutions to ensure the uniform distribution of silver nanoparticles in the polyurethane solution;

[0026] Electrospinning: The mixed solution is electrospun to prepare polyurethane nanofibers. During the electrospinning process, silver nanoparticles are formed in-situ and embedded on the surface of the polyurethane nanofibers;

[0027] Post-treatment: The nanofiber membrane layer obtained by electrospinning is post-treated to obtain a silver-loaded fluorinated polyurethane nanofiber membrane.

[0028] As a further description of the above technical solution:

[0029] The post-treatment of the nanofiber membrane layer specifically includes: adding the prepared nanofiber membrane layer into a sealed container, injecting an appropriate amount of ethanol solvent, heating to an appropriate temperature to generate steam, sealing the container, evacuating to allow the solvent steam to fully penetrate into the nanofiber membrane layer, and opening the sealed container to wait for the steam in the sealed container to naturally volatilize.

[0030] As a further description of the above technical solution:

[0031] The incorporation of the natural antibacterial compound honokiol into the silver-loaded fluorinated polyurethane nanofiber membrane, coating the silver-loaded fluorinated polyurethane nanofiber membrane with a polydopamine coating, and spraying the prepared sustained-release microparticles of honokiol on the surface of the coating.

[0032] As a further description of the above technical solution:

[0033] The sterilization treatment of the antibacterial substrate and the combined packaging include: covering an antibacterial film on the surface of the coating and covering a breathable adhesive film layer on the surface of the substrate.

[0034] As a further description of the above technical solution:

[0035] A medical antibacterial sponge, including a nanofiber membrane layer, a coating is sprayed at the bottom of the nanofiber membrane layer, a negative pressure permeation layer is connected to the top of the nanofiber membrane layer, a plurality of ventilation holes are opened through the bottom of the negative pressure permeation layer, and a hydrophilic adsorption layer is connected to the top of the negative pressure permeation layer. A breathable adhesive film layer is connected to the top of the hydrophilic adsorption layer, and the ventilation holes pass through the hydrophilic adsorption layer and extend to the top breathable adhesive film layer.

[0036] As a further description of the above technical solution:

[0037] The hydrophilic adsorption layer is connected to the negative pressure permeation layer through pore penetration.

[0038] As a further description of the above technical solution:

[0039] The coating is a polydopamine coating of sustained-release microparticles of honokiol.

[0040] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0041] 1. In the present invention, during the preparation of polyurethane nanofibers by electrospinning with silver ions as precursors, silver nanoparticles are loaded and embedded on the surface of the polyurethane nanofibers. Moreover, sustained-release microparticles of honokiol can be adsorbed by coating a dopamine coating on the surface of the polyurethane nanofibers, and the sustained antibacterial effect of the polyurethane nanofiber film layer can be extended by the sustained-release honokiol.

[0042] 2. In the present invention, through the cooperation of the negative pressure permeation layer and the nanofiber film layer, when the wound contacts the nanofiber film layer, the wound exudate can be guided to the hydrophilic adsorption layer through the highly permeable negative pressure permeation layer, thereby avoiding the accumulation of wound exudate on the nanofiber film layer, and improving the dressing stability of the antibacterial sponge in combination with the antibacterial effect of the nanofiber film layer. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 It is a process flow diagram of a preparation method of a medical antibacterial sponge proposed by the present invention;

[0044] Figure 2 It is a schematic cross-sectional structure diagram of a medical antibacterial sponge proposed by the present invention.

[0045] LEGEND DESCRIPTION:

[0046] 1. Breathable adhesive film layer; 2. Coating; 3. Nanofiber film layer; 4. Negative pressure permeation layer; 5. Ventilation holes; 6. Hydrophilic adsorption layer. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0047] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0048] Embodiment 1

[0049] Please refer to Figure 1 , the present invention provides a technical solution: a preparation method of a medical antibacterial sponge, including the following steps:

[0050] Prepare silver-loaded polyurethane nanofibers by electrospinning;

[0051] Incorporate the natural antibacterial compound honokiol into the silver-loaded fluorinated polyurethane nanofiber film to obtain an antibacterial substrate;

[0052] Perform sterilization treatment on the antibacterial substrate and carry out combined packaging.

[0053] The preparation method of the sustained-release granules of honokiol comprises:

[0054] preparing an inner aqueous phase: dissolving honokiol in water to form an inner aqueous phase;

[0055] Preparation of colostrum: dissolving polylactic acid-co-glycolic acid in an organic solvent, dichloromethane, then adding the inner water phase to the polylactic acid-co-glycolic acid organic solution, adding the mixture to a vacuum stirring kettle, and vacuuming and stirring for 2 minutes to form colostrum;

[0056] Preparation of double emulsion: Colostrum is transferred to an aqueous solution containing an emulsifier in the external aqueous phase, and a double emulsion is formed by further stirring;

[0057] Spray drying: The emulsion is dried through a spray drying device to remove the organic solvent, so that the polylactic acid-glycolic acid copolymer is solidified during the spraying process to form microspheres;

[0058] Post-treatment: The microspheres were collected by centrifugal filtration, washed with deionized water to remove uncoated honokiol and residual organic solvent, and finally freeze-dried to obtain solid sustained-release microspheres. The speed of the vacuum stirring kettle was 1500 r / min.

[0059] The preparation method of the silver-loaded fluorinated polyurethane nanofiber membrane comprises:

[0060] Prepare polyurethane solution: dissolve polyurethane in dimethylformamide;

[0061] Adding silver precursor: adding silver nitrate as a silver precursor to the polyurethane solution;

[0062] Adding reducing agent: adding tannic acid as a reducing agent to promote the reduction of silver ions and form silver nanoparticles;

[0063] Mixing and stirring: the above solutions are fully mixed and stirred to ensure that the silver nanoparticles are evenly distributed in the polyurethane solution;

[0064] Electrospinning: The mixed solution is electrospun to prepare polyurethane nanofibers. During the electrospinning process, silver nanoparticles are formed in situ and embedded into the surface of polyurethane nanofibers.

[0065] Post-treatment: post-treating the nanofiber membrane layer obtained by electrospinning to obtain a silver-loaded fluorinated polyurethane nanofiber membrane;

[0066] The post-treatment of the nanofiber membrane layer specifically includes: adding the prepared nanofiber membrane layer into a sealed container, injecting an appropriate amount of ethanol solvent, heating to an appropriate temperature to generate steam, sealing the container, evacuating the container to allow the solvent vapor to fully penetrate into the nanofiber membrane layer, opening the sealed container and waiting for the steam in the sealed container to evaporate naturally;

[0067] The natural antibacterial compound honokiol is incorporated into a silver-loaded fluorinated polyurethane nanofiber membrane, a polydopamine coating is applied to the silver-loaded fluorinated polyurethane nanofiber membrane, and the prepared sustained-release microparticles of honokiol are sprayed onto the surface of the coating. The natural antibacterial compound honokiol is incorporated into the silver-loaded fluorinated polyurethane nanofiber membrane, a polydopamine coating is applied to the silver-loaded fluorinated polyurethane nanofiber membrane, and the prepared sustained-release microparticles of honokiol are sprayed onto the surface of the coating.

[0068] Example 2

[0069] Different from Example 1, a preparation method of a medical antibacterial sponge includes the following steps:

[0070] Prepare silver-loaded polyurethane nanofibers by electrospinning;

[0071] Incorporate the natural antibacterial compound honokiol into the silver-loaded fluorinated polyurethane nanofiber membrane to obtain an antibacterial substrate;

[0072] Perform a sterilization treatment on the antibacterial substrate and carry out combined packaging.

[0073] The preparation method of the sustained-release granules of honokiol includes:

[0074] Prepare the inner aqueous phase: Dissolve honokiol in water to form the inner aqueous phase;

[0075] Prepare the primary emulsion: Dissolve poly(lactic-co-glycolic acid) in the organic solvent dichloromethane, then add the inner aqueous phase to the poly(lactic-co-glycolic acid) organic solution, and after adding it to a vacuum stirring kettle, evacuate and stir for 4 min to form the primary emulsion;

[0076] Prepare the double emulsion: Transfer the primary emulsion to an aqueous solution containing an emulsifier in the outer aqueous phase and form a double emulsion by further stirring;

[0077] Spray drying: Dry the double emulsion through a spray drying device to remove the organic solvent, and solidify the poly(lactic-co-glycolic acid) during the spraying process to form microspheres;

[0078] Post-treatment: Centrifuge and filter to collect the microspheres, wash them with deionized water to remove the uncoated honokiol and residual organic solvents, and finally freeze-dry to obtain solid sustained-release microspheres. The rotation speed of the vacuum stirring kettle is 1600 r / min.

[0079] The preparation method of the silver-loaded fluorinated polyurethane nanofiber membrane includes:

[0080] Prepare a polyurethane solution: Dissolve polyurethane in dimethylformamide;

[0081] Add a silver precursor: Add silver nitrate as a silver precursor to the polyurethane solution;

[0082] Adding reducing agent: adding tannic acid as a reducing agent to promote the reduction of silver ions and form silver nanoparticles;

[0083] Mixing and stirring: the above solutions are fully mixed and stirred to ensure that the silver nanoparticles are evenly distributed in the polyurethane solution;

[0084] Electrospinning: The mixed solution is electrospun to prepare polyurethane nanofibers. During the electrospinning process, silver nanoparticles are formed in situ and embedded into the surface of polyurethane nanofibers.

[0085] Post-treatment: post-treating the nanofiber membrane layer obtained by electrospinning to obtain a silver-loaded fluorinated polyurethane nanofiber membrane;

[0086] The post-treatment of the nanofiber membrane layer specifically includes: adding the prepared nanofiber membrane layer into a sealed container, injecting an appropriate amount of ethanol solvent, heating to an appropriate temperature to generate steam, sealing the container, evacuating the container to allow the solvent vapor to fully penetrate into the nanofiber membrane layer, opening the sealed container and waiting for the steam in the sealed container to evaporate naturally;

[0087] The natural antibacterial compound honokiol is incorporated into a silver-loaded fluorinated polyurethane nanofiber membrane, a polydopamine coating is coated on the silver-loaded fluorinated polyurethane nanofiber membrane, and the prepared sustained-release microparticles of honokiol are sprayed on the surface of the coating. The natural antibacterial compound honokiol is incorporated into a silver-loaded fluorinated polyurethane nanofiber membrane, a polydopamine coating is coated on the silver-loaded fluorinated polyurethane nanofiber membrane, and the prepared sustained-release microparticles of honokiol are sprayed on the surface of the coating.

[0088] Embodiment 3

[0089] A method for preparing a medical antibacterial sponge comprises the following steps:

[0090] Preparation of silver-loaded polyurethane nanofibers by electrospinning;

[0091] The natural antimicrobial compound honokiol was incorporated into silver-loaded fluorinated polyurethane nanofiber membranes to obtain an antimicrobial substrate;

[0092] The antibacterial substrate is sterilized and combined packaging is performed.

[0093] The preparation method of the sustained-release granules of honokiol comprises:

[0094] preparing an inner aqueous phase: dissolving honokiol in water to form an inner aqueous phase;

[0095] Preparation of colostrum: dissolving polylactic acid-co-glycolic acid in an organic solvent, dichloromethane, then adding the inner water phase to the polylactic acid-co-glycolic acid organic solution, adding the mixture to a vacuum stirring kettle, and vacuuming and stirring for 5 minutes to form colostrum;

[0096] Preparation of double emulsion: Transfer the primary emulsion to an aqueous solution containing an emulsifier in the external aqueous phase, and form a double emulsion by further stirring;

[0097] Spray drying: Dry the double emulsion through a spray drying device to remove the organic solvent, and solidify the poly(lactic-co-glycolic acid) copolymer during the spraying process to form microspheres;

[0098] Post-treatment: Centrifuge and filter to collect the microspheres, wash them with deionized water to remove uncoated honokiol and residual organic solvents, and finally freeze-dry to obtain solid sustained-release microspheres. The rotation speed of the vacuum stirring kettle is 1800 r / min.

[0099] The preparation method of the silver-loaded fluorinated polyurethane nanofiber membrane includes:

[0100] Preparation of polyurethane solution: Dissolve polyurethane in dimethylformamide;

[0101] Add silver precursor: Add silver nitrate as the silver precursor to the polyurethane solution;

[0102] Add reducing agent: Add tannic acid as the reducing agent to promote the reduction of silver ions to form silver nanoparticles;

[0103] Mixing and stirring: Thoroughly mix and stir the above solutions to ensure the uniform distribution of silver nanoparticles in the polyurethane solution;

[0104] Electrospinning: Electrospin the mixed solution to prepare polyurethane nanofibers. During the electrospinning process, silver nanoparticles will be formed in-situ and embedded on the surface of the polyurethane nanofibers;

[0105] Post-treatment: Post-treat the nanofiber membrane layer obtained by electrospinning to obtain a silver-loaded fluorinated polyurethane nanofiber membrane;

[0106] The post-treatment of the nanofiber membrane layer specifically includes: Add the prepared nanofiber membrane layer to a sealed container, inject an appropriate amount of ethanol solvent, heat to an appropriate temperature to generate steam, seal the container, evacuate to allow the solvent steam to fully penetrate into the nanofiber membrane layer, and open the sealed container to wait for the steam in the sealed container to naturally volatilize;

[0107] Incorporate the natural antibacterial compound honokiol into the silver-loaded fluorinated polyurethane nanofiber membrane, coat a polydopamine coating on the silver-loaded fluorinated polyurethane nanofiber membrane, and spray the prepared sustained-release microparticles of honokiol on the surface of the coating. Incorporate the natural antibacterial compound honokiol into the silver-loaded fluorinated polyurethane nanofiber membrane, coat a polydopamine coating on the silver-loaded fluorinated polyurethane nanofiber membrane, and spray the prepared sustained-release microparticles of honokiol on the surface of the coating.

[0108] Example 4

[0109] Please refer to Figure 2 , a medical antibacterial sponge, including a nanofiber membrane layer 3, a coating 2 is sprayed on the bottom of the nanofiber membrane layer 3, a negative pressure permeation layer 4 is connected to the top of the nanofiber membrane layer 3, a plurality of ventilation holes 5 are formed through the bottom of the negative pressure permeation layer 4, and a hydrophilic adsorption layer 6 is connected to the top of the negative pressure permeation layer 4. A breathable adhesive film layer 1 is connected to the top of the hydrophilic adsorption layer 6, and the ventilation holes 5 pass through the hydrophilic adsorption layer 6 and extend to the top breathable adhesive film layer 1. The hydrophilic adsorption layer 6 is connected with the negative pressure permeation layer 4 through pore permeation. The coating 2 is a polydopamine coating 2 of magnolol sustained-release microparticles.

[0110] Specifically: the negative pressure permeation layer can be a hydrophilic foaming material, such as polyacrylamide gel and hydrophilic rubber fiber.

[0111] In the present invention, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance; the term "plurality" refers to two or more, unless otherwise clearly defined. Terms such as "installation", "connection", "connection", "fixation" and other terms should be understood in a broad sense. For example, "connection" can be a fixed connection, a detachable connection, or an integral connection; "connection" can be a direct connection or an indirect connection through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0112] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A method for preparing a medical antibacterial sponge, characterized in that: The following steps are involved: Preparation of silver-loaded polyurethane nanofibers by electrospinning; The natural antimicrobial compound honokiol was incorporated into silver-loaded fluorinated polyurethane nanofiber membranes to obtain an antimicrobial substrate; The antibacterial substrate is sterilized and combined packaging is performed.

2. The method for preparing a medical antibacterial sponge according to claim 1, characterized in that: The preparation method of the sustained-release granules of honokiol comprises: preparing an inner aqueous phase: dissolving honokiol in water to form an inner aqueous phase; Preparation of colostrum: dissolving polylactic acid-co-glycolic acid in an organic solvent, dichloromethane, then adding the inner water phase to the polylactic acid-co-glycolic acid organic solution, adding the mixture to a vacuum stirring kettle, and vacuuming and stirring for 2-5 minutes to form colostrum; Preparation of double emulsion: Colostrum is transferred to an aqueous solution containing an emulsifier in the external aqueous phase, and a double emulsion is formed by further stirring; Spray drying: The emulsion is dried through a spray drying device to remove the organic solvent, so that the polylactic acid-glycolic acid copolymer is solidified during the spraying process to form microspheres; Post-treatment: The microspheres were collected by centrifugal filtration and washed with deionized water to remove uncoated honokiol and residual organic solvent, and finally freeze-dried to obtain solid sustained-release microspheres.

3. The method for preparing a medical antibacterial sponge according to claim 2, characterized in that: The rotation speed of the vacuum stirring kettle is 1500-1800r / mi.

4. The method for preparing a medical antibacterial sponge according to claim 1, characterized in that: The preparation method of the silver-loaded fluorinated polyurethane nanofiber membrane comprises: Prepare polyurethane solution: dissolve polyurethane in dimethylformamide; Adding silver precursor: adding silver nitrate as a silver precursor to the polyurethane solution; Adding reducing agent: adding tannic acid as a reducing agent to promote the reduction of silver ions and form silver nanoparticles; Mixing and stirring: the above solutions are fully mixed and stirred to ensure that the silver nanoparticles are evenly distributed in the polyurethane solution; Electrospinning: The mixed solution is electrospun to prepare polyurethane nanofibers. During the electrospinning process, silver nanoparticles are formed in situ and embedded into the surface of polyurethane nanofibers. Post-treatment: The nanofiber membrane layer obtained by electrospinning is post-treated to obtain a silver-loaded fluorinated polyurethane nanofiber membrane.

5. The method for preparing a medical antibacterial sponge according to claim 4, characterized in that: The post-processing of the nanofiber membrane layer specifically includes: adding the prepared nanofiber membrane layer into a closed container, injecting an appropriate amount of ethanol solvent, heating to an appropriate temperature to generate steam, sealing the container, evacuating the container to allow the solvent vapor to fully penetrate into the nanofiber membrane layer, opening the closed container and waiting for the steam in the closed container to evaporate naturally.

6. The method for preparing a medical antibacterial sponge according to claim 1, characterized in that: The method comprises mixing the natural antibacterial compound honokiol into a silver-loaded fluorinated polyurethane nanofiber membrane, coating the silver-loaded fluorinated polyurethane nanofiber membrane with a polydopamine coating, and spraying the prepared sustained-release particles of honokiol on the surface of the coating.

7. The method for preparing a medical antibacterial sponge according to claim 1, characterized in that: The sterilization treatment of the antibacterial substrate and the combined packaging include: covering the antibacterial film on the surface of the coating layer and covering the air-permeable adhesive film layer on the surface of the base layer.

8. A medical antibacterial sponge prepared by the method for preparing the medical antibacterial sponge according to claims 1-7, characterized in that: It includes a nanofiber membrane layer, a coating is sprayed on the bottom of the nanofiber membrane layer, a negative pressure permeation layer is connected to the top of the nanofiber membrane layer, a plurality of air holes are opened through the bottom of the negative pressure permeation layer, a hydrophilic adsorption layer is connected to the top of the negative pressure permeation layer, a breathable adhesive membrane layer is connected to the top of the hydrophilic adsorption layer, and the air holes extend through the hydrophilic adsorption layer to the top breathable adhesive membrane layer.

9. The medical antibacterial sponge according to claim 8, characterized in that: The hydrophilic adsorption layer is in pore-penetratingly connected with the negative pressure permeation layer.

10. The medical antibacterial sponge according to claim 8, characterized in that: The coating is a polydopamine coating of sustained-release particles of honokiol.

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