Breeding method for enhancing body color of Dongmaotai

Abstract

The invention discloses a culture method for enhancing the body color of Dongmaotai, and relates to the technical field of aquaculture. The method comprises the following steps: (1) illumination induction: alternately irradiating by adopting a light source white-red-blue-yellow, (2) feeding with a toner: feeding with basal feed at the same time, and (3) water flow stimulation: regulating physiological metabolism, nutrition absorption and environmental adaptability to realize the purpose of improving the quality of the feed by regulating the physiological metabolism, nutrition absorption and environmental adaptability. The method is beneficial to enhancing the body color of the east astroplatia and improving the economic value of the east astroplatia.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and particularly relates to a cultivation method for enhancing the body color of Epinephelus akaara. Background Art

[0002] The scientific name of Epinephelus akaara is Plectropomus leopardus, which belongs to Perciformes, Percoidei, Serranidae, Epinephelinae, and Plectropomus, and is a warm-water reef carnivorous fish. Its color is blue, red, brown, yellow, etc. Its body shape is thinner and longer than that of general groupers, and its head is small. The body and head are olive to reddish-brown, and the head, body, and odd fins are covered with small blue dots. The pectoral fins are red, and there is an indistinct dark band at the trailing edge of the caudal fin. Its color is bright, with dark pupils in its blue eyes, and the fine dots on its body resemble stars in the sky, so it is commonly known as "star grouper", and "east" because it is produced in the Dongsha Islands in the eastern part of the South China Sea. Epinephelus akaara has white, tender, and nutritious meat, and belongs to precious and high-grade edible fish, which is deeply favored by consumers. However, due to the prevalence of the current high-density aquaculture mode, combined with the deterioration of the aquaculture environment and the degradation of germplasm of Epinephelus akaara, the body color of artificially cultured Epinephelus akaara has seriously degenerated, the body color has become lighter, which seriously affects the quality and market price of artificially cultured Epinephelus akaara. Therefore, there is an urgent need to provide a cultivation method that can significantly enhance the body color of Epinephelus akaara. Summary of the Invention

[0003] Aiming at the above-mentioned defects of the prior art, the present invention proposes a cultivation method for enhancing the body color of Epinephelus akaara to solve the problems raised in the above background art.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A cultivation method for enhancing the body color of Epinephelus akaara, comprising the following steps:

[0006] (1) Light induction: Alternately irradiate with light sources of white - red - blue - yellow;

[0007] (2) Feeding of color enhancer: Feed simultaneously with the basic feed. The components of the color enhancer include camellia polysaccharide, sargassum polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidinoacetic acid, and sodium butyrate; when the pH value of the aquaculture water is 7.2 - 7.7, the feeding amount of the color enhancer is 0.8 - 1.5% of the fish body weight; when the pH value of the aquaculture water is 7.8 - 8.2, the feeding amount of the color enhancer is 0.15 - 0.35% of the fish body weight; when the pH value of the aquaculture water is 8.3 - 8.6, the feeding amount of the color enhancer is 0.50 - 1.0% of the fish body weight;

[0008] (3) Water flow stimulation: Keep the water body flowing at a flow rate of 0.10 - 0.15 m / s for 2 - 4 h every day, at a flow rate of 0.20 - 0.30 m / s for 1 - 3 h, and at a flow rate of 0.35 - 0.40 m / s for 0.5 - 1.5 h, and stop flowing water for the rest of the time.

[0009] Preferably, the irradiation time of the white light source in step (1) is the same as the total irradiation time of the red-blue-yellow light sources.

[0010] Preferably, in step (1), the irradiation time of the white light source is 06:00-18:00, the irradiation time of the red light source is 18:00-02:00, the irradiation time of the blue light source is 02:00-04:00, and the irradiation time of the yellow light source is 04:00-06:00.

[0011] Preferably, in step (1), the irradiation intensity of the white light source is 100-150 lux, the irradiation intensity of the red light source is 500-1000 lux, the irradiation intensity of the blue light source is 300-500 lux, and the irradiation intensity of the yellow light source is 200-300 lux.

[0012] Preferably, the wavelength range of the white light source is 500-600 nm, the wavelength range of the red light source is 610-650 nm, the wavelength range of the blue light source is 450-480 nm, and the wavelength range of the yellow light source is 580-590 nm.

[0013] Preferably, the components of the basic feed in step (2) include, by weight, 30 to 40 parts of white fish meal, 20 to 35 parts of earthworm meal, 10 to 15 parts of corn gluten meal, 7 to 12 parts of soybean meal, 5 to 10 parts of soybean oil, 2 to 7 parts of choline chloride and 1 to 6 parts of vitamin C.

[0014] Preferably, the amount of the basic feed in step (2) is 3-5% of the fish body weight.

[0015] Preferably, the color enhancer components in step (2) are mixed and sieved by weight, extruded, puffed, granulated and dried to obtain the color enhancer, which includes 20 to 30 parts of Camellia chrysantha polysaccharide, 20 to 25 parts of Sargassum fusiformis polysaccharide, 10 to 18 parts of fucoxanthin, 8 to 12 parts of scallop peptide, 4 to 10 parts of silymarin, 1 to 3 parts of guanidinoacetic acid and 1 to 3 parts of sodium butyrate.

[0016] Preferably, the components of the color enhancer in step (2) include, by weight, 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate.

[0017] Preferably, in step (2), when the pH value of the aquaculture water is 7.2-7.7, the amount of the color enhancer fed is 1.2% of the fish body weight; when the pH value of the aquaculture water is 7.8-8.2, the amount of the color enhancer fed is 0.30% of the fish body weight; when the pH value of the aquaculture water is 8.3-8.6, the amount of the color enhancer fed is 0.80% of the fish body weight.

[0018] Preferably, in step (3), the water body maintains a flow rate of 0.12 m / s for 3 h per day, a flow rate of 0.25 m / s for 2 h, and a flow rate of 0.38 m / s for 1.0 h, and the water flow stops for the rest of the time. The water flow rate is controlled by a 500-watt variable-frequency water pump installed in the pool.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] (1) Appropriate alternating light induction can promote the activity of chromatophores in the body of Epinephelus coioides, especially the synthesis and deposition of carotenoids. By regulating the photoperiod and light intensity and simulating the light changes in the habitat environment, the stress response of the fish body can be reduced, thereby maintaining the stability and vividness of the body color.

[0021] (2) Adding a color enhancer containing carotenoids (such as fucoxanthin) to the feed can directly promote the accumulation of pigments on the fish body surface. At the same time, by correlating the feeding amount of the color enhancer with the pH of the aquaculture water and using specific aquaculture feeding techniques and feeding strategies, the digestion and utilization rate of protein in the basic feed can be improved, the nutrient absorption efficiency can be enhanced, and the pigment metabolism can be accelerated or the synthesis and color development of pigments can be indirectly supported.

[0022] (3) Moderate circulating water flow stimulation can enhance the exercise amount of the Epinephelus coioides fish body, promote blood circulation and metabolism. This process helps the pigments to be evenly distributed, and at the same time reduces the dull body color caused by environmental stress, making the fish body show a natural bright color. Specific embodiments

[0023] The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0024] Example 1

[0025] A breeding method for enhancing the body color of Epinephelus coioides includes the following steps:

[0026] (1) Construction of the breeding pond: Build a breeding pond indoors and disinfect the breeding pond with a mixed solution of bleaching powder and chlorine dioxide before releasing the fry.

[0027] (2) Release of fry: The water temperature in the breeding pond is 22 - 29 °C, the salinity is 25 - 32 ‰, and the dissolved oxygen is above 5 mg / L. Select Epinephelus coioides fry with regular specifications, strong physique, and no diseases or injuries, with a size of 2.5 - 3 cm, and release them into the breeding pond at a density of 100 - 200 tails / m 3 . Soak the Epinephelus coioides fry in brine for 10 min before putting them in for fry disinfection.

[0028] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 100 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 500 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 300 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 200 lux.

[0029] (4) Color enhancer feeding: feeding at the same time as the basic feed, 3 times a day, feeding time is 09:00, 13:00 and 18:00, the components of the color enhancer by weight include 20 parts of Camellia chrysantha polysaccharide, 20 parts of Sargassum fusiformis polysaccharide, 10 parts of fucoxanthin, 8 parts of scallop peptide, 4 parts of silymarin, 1 part of guanidinoacetic acid and 1 part of sodium butyrate; when the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 0.8% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the feeding amount of the color enhancer is 0.15% of the fish body weight; when the pH value of the aquaculture water is 8.3-8.6, the feeding amount of the color enhancer is 0.50% of the fish body weight; the feeding amount of the basic feed is 3% of the fish body weight, and the components of the basic feed are calculated by weight, including 30 parts of white fish meal, 20 parts of earthworm meal, 10 parts of corn gluten meal, 7 parts of soybean meal, 5 parts of soybean oil, 2 parts of choline chloride and 1 part of vitamin C;

[0030] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.10 m / s for 2 h, 0.20 m / s for 1 h, and 0.35 m / s for 0.5 h every day. The water flow is stopped at other times.

[0031] Example 2

[0032] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0033] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0034] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0035] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 150 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 1000 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 500 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 300 lux.

[0036] (4) Color enhancer feeding: feeding at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 30 parts of Camellia chrysantha polysaccharide, 25 parts of Sargassum fusiformis polysaccharide, 18 parts of fucoxanthin, 12 parts of scallop peptide, 10 parts of silymarin, 3 parts of guanidinoacetic acid and 3 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.5% of the fish body weight; When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed at a rate of 0.35% of the fish body weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed at a rate of 1.0% of the fish body weight; the basic feed is fed at a rate of 5% of the fish body weight, and the basic feed includes, by weight, 40 parts of white fish meal, 35 parts of earthworm meal, 15 parts of corn gluten meal, 12 parts of soybean meal, 10 parts of soybean oil, 7 parts of choline chloride and 6 parts of vitamin C;

[0037] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.15 m / s for 4 h, 0.30 m / s for 3 h, and 0.40 m / s for 1.5 h every day. The water flow is stopped at other times.

[0038] Example 3

[0039] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0040] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0041] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0042] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0043] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0044] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0045] Comparative Example 1

[0046] This comparative example is different from Example 3 in that there is no red-blue-yellow induction light source, and the other components and steps remain unchanged.

[0047] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0048] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0049] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0050] (3) Light induction: After releasing the fry, use a white light source to irradiate the whole day, and the irradiation intensity is 120 lux;

[0051] (4) Feeding of color enhancer: Feed at the same time as the basic feed, 3 times a day, and the feeding times are 09:00, 13:00 and 18:00. The components of the color enhancer are calculated by weight, including 25 parts of camellia polysaccharide, 22 parts of sargassum polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate; when the pH value of the aquaculture water is 7.2 - 7.7, the feeding amount of the color enhancer is 1.2% of the fish body weight; when the pH value of the aquaculture water is 7.8 - 8.2, the feeding amount of the color enhancer is 0.30% of the fish body weight; when the pH value of the aquaculture water is 8.3 - 8.6, the feeding amount of the color enhancer is 0.80% of the fish body weight; the feeding amount of the basic feed is 4% of the fish body weight, and the components of the basic feed are calculated by weight, including 35 parts of white fish meal, 30 parts of earthworm powder, 12 parts of corn protein powder, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0052] (5) Water flow stimulation: The water body maintains a flow rate of 0.12 m / s for 3 h every day, a flow rate of 0.25 m / s for 2 h, and a flow rate of 0.38 m / s for 1.0 h, and the water stops flowing for the rest of the time.

[0053] Comparative Example 2

[0054] The difference between this comparative example and Example 3 is that there is no red light source induction, and the other components and steps remain unchanged.

[0055] A breeding method for enhancing the body color of groupers, comprising the following steps:

[0056] (1) Construction of breeding ponds: Build breeding ponds indoors, and disinfect the breeding ponds with a mixed solution of bleaching powder and chlorine dioxide before releasing the fry;

[0057] (2) Releasing fry: The water temperature of the breeding pond is 22 - 29 °C, the salinity is 25 - 32 ‰, and the dissolved oxygen is above 5 mg / L. Select groupers fry with regular specifications, strong physique, and no diseases or injuries, with a size of 2.5 - 3 cm, and release them into the breeding pond at a density of 100 - 200 tails / m 3 , and soak the groupers fry in brine for 10 min before putting them in for fry disinfection;

[0058] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the blue-yellow light source. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the blue light source is 18:00-24:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 24:00-06:00, and the irradiation intensity is 250 lux.

[0059] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0060] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0061] Comparative Example 3

[0062] This comparative example is different from Example 3 in that there is no blue light source for induction, and the other components and steps remain unchanged.

[0063] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0064] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0065] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0066] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the yellow light source is 02:00-06:00, and the irradiation intensity is 250 lux.

[0067] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0068] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0069] Comparative Example 4

[0070] This comparative example is different from Example 3 in that there is no yellow induction light source, and the other components and steps remain unchanged.

[0071] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0072] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0073] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0074] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, and blue. The irradiation time of the white light source is the same as the total irradiation time of the red, blue, and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-06:00, and the irradiation intensity is 400 lux.

[0075] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0076] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0077] Comparative Example 5

[0078] This comparative example is different from Example 3 in that the irradiation time of the white light source and the total irradiation time of the red-blue-yellow light sources are changed, and the other components and steps remain unchanged.

[0079] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0080] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0081] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0082] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is higher than the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is from 06:00 to 21:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is from 21:00 to 04:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is from 04:00 to 05:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is from 05:00 to 06:00, and the irradiation intensity is 250 lux.

[0083] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0084] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0085] Comparative Example 6

[0086] This comparative example differs from Example 3 in that no toner is fed, and the other ingredients and steps remain unchanged.

[0087] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0088] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0089] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0090] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0091] (4) Basic feed feeding: feeding three times a day, at 09:00, 13:00 and 18:00, the basic feed feeding amount is 4% of the fish body weight, and the components of the basic feed, by weight, include 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0092] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0093] Comparative Example 7

[0094] The difference between this comparative example and Example 3 is that the color enhancer component does not include Camellia chrysantha polysaccharide and Sargassum fusiformis polysaccharide, and the other components and steps remain unchanged.

[0095] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0096] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0097] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0098] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0099] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the aquaculture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when the pH value of the aquaculture water is When the pH value of the aquaculture water is between 8.3 and 8.6, the amount of the color enhancer fed is 0.30% of the fish body weight; when the pH value of the aquaculture water is between 8.3 and 8.6, the amount of the color enhancer fed is 0.80% of the fish body weight; the amount of the basic feed fed is 4% of the fish body weight, and the components of the basic feed, by weight, include 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0100] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0101] Comparative Example 8

[0102] The difference between this comparative example and Example 3 is that the amount of the color enhancer fed does not change with the pH value of the aquaculture water, and the other components and steps remain unchanged.

[0103] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0104] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0105] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0106] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0107] (4) Color enhancer feeding: feeding at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00, the components of the color enhancer by weight include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate; the color enhancer feeding amount is 0.80% of the fish body weight; the basic feed feeding amount is 4% of the fish body weight, the basic feed components by weight include 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0108] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h, 0.25 m / s for 2 h, and 0.38 m / s for 1.0 h every day. The water flow is stopped at other times.

[0109] Comparative Example 9

[0110] This comparative example differs from Example 3 in that there is no water flow stimulation, and the other components and steps remain unchanged.

[0111] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0112] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0113] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0114] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0115] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0116] Comparative Example 10

[0117] This comparative example differs from Example 3 in that there is no multiple water flow stimulation, and the other components and steps remain unchanged.

[0118] A breeding method for enhancing the body color of Oriental starfish comprises the following steps:

[0119] (1) Construction of breeding pond: Build breeding pond indoors and disinfect the breeding pond with a bleaching powder-chlorine dioxide mixed solution before stocking the fry;

[0120] (2) Stocking of fry: The water temperature of the breeding pond is 22-29°C, the salinity is 25-32‰, and the dissolved oxygen is above 5 mg / L. Select 2.5-3 cm red snapper fry with uniform size, strong physique, and no disease or injury and stock them in the breeding pond. Stock 100-200 fry / m 3 , soak the grouper fry in salt water for 10 minutes before putting them in to disinfect the fry;

[0121] (3) Light induction: After the fry are released, the light source is alternately irradiated with white, red, blue and yellow. The irradiation time of the white light source is the same as the total irradiation time of the red, blue and yellow light sources. The irradiation time of the white light source is 06:00-18:00, and the irradiation intensity is 120 lux. The irradiation time of the red light source is 18:00-02:00, and the irradiation intensity is 600 lux. The irradiation time of the blue light source is 02:00-04:00, and the irradiation intensity is 400 lux. The irradiation time of the yellow light source is 04:00-06:00, and the irradiation intensity is 250 lux.

[0122] (4) Color enhancer feeding: Feed at the same time as the basic feed, 3 times a day, at 09:00, 13:00 and 18:00. The components of the color enhancer, by weight, include 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate. When the pH value of the culture water is 7.2-7.7, the color enhancer feeding amount is 1.2% of the fish body weight; when When the pH value of the aquaculture water is 7.8-8.2, the color enhancer is fed in an amount of 0.30% of the fish weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer is fed in an amount of 0.80% of the fish weight; the basic feed is fed in an amount of 4% of the fish weight, and the basic feed comprises, by weight, 35 parts of white fish meal, 30 parts of earthworm meal, 12 parts of corn gluten meal, 10 parts of soybean meal, 8 parts of soybean oil, 5 parts of choline chloride and 4 parts of vitamin C;

[0123] (5) Water flow stimulation: The water body is maintained at a flow rate of 0.12 m / s for 3 h every day, and the water flow is stopped at other times.

[0124] Experimental example Experimental index determination

[0125] 1.1 Test Object

[0126] A control group was set up for the grouper after 60 days of cultivation using the cultivation methods of Examples 1 to 3 and Comparative Examples 1 to 10 (application number 202210800228.3, patent subject: an indoor seedling cultivation method for grouper).

[0127] 1.2 Test Method

[0128] 1.2.1 Growth indicators

[0129] Growth rate (MG) = (W t -W0) / W0×100%, where W t is the average weight of the fish at the end (g), W0 is the average weight of the fish at the beginning (g); Feed coefficient (FCR) = F / (W t-W0), where F is the food intake. The experimental data are expressed as the mean ± standard deviation and statistically analyzed using SPSS 19.0 software.

[0130] 1.2.2 Body color index

[0131] The brightness (L ＊ ) of the abdomen of the groupers is detected using a CR-400 color difference meter, as well as the red-green value (a ＊ ) and the yellow-blue value (b ＊ ). The larger the value of L ＊ , the brighter the body color; a positive value of a ＊ represents a tendency towards red, while a negative value represents a tendency towards green; a positive value of b ＊ represents a tendency towards yellow, while a negative value represents a tendency towards blue. Note that when measuring, first dry the surface moisture of the fish body with absorbent paper, and then closely attach the probe of the color difference meter to the part of the experimental fish to be measured to read the results.

[0132] 1.3 Experimental results

[0133] 1.3.1 The growth rate and feed coefficient results are shown in Table 1, and the body color index results are shown in Table 2;

[0134] Table 1 Growth indicators after 60 days of cultivation

[0135]

[0136]

[0137] Table 2 Abdominal body color index after 60 days of cultivation

[0138]

[0139] As can be seen from Table 1 and Table 2, through the synergistic effect of light induction, color enhancer feeding, and water flow stimulation, it can be significantly found that while improving the growth rate and feed coefficient of groupers, the body color can also be enhanced, making the body color of groupers bright, and significantly improving the economic value of groupers. The effect of Example 3 is the best and is better than that of the control group. By comparing Example 3 with Comparative Examples 1-4, it can be known that alternating irradiation with white - red - blue - yellow can improve the body color of groupers more than a single light source. The alternating use of multiple light qualities stimulates different photosensitive receptors, activates multiple gene pathways related to pigment synthesis, promotes the comprehensive expression of cytochromes. The dynamic light environment will enhance the sensitivity of groupers to the photoperiod, regulate the secretion of hormones related to body color, thereby promoting the differentiation and migration of chromatophores, which is beneficial to enhancing the body color of groupers. At the same time, it is necessary to master the irradiation time of each light source to avoid only activating some metabolic pathways, which may inhibit the generation of other pigments; by comparing Example 3 with Comparative Examples 6-8, it can be known that through the synergistic interaction of the active components of each component of the color enhancer, the color enhancer can not only enhance the body color, but also increase the proportion of feed converted into the meat of groupers, further improving the quality of the fish meat. In particular, camellia polysaccharide and sargassum fusiforme polysaccharide are applied to the improvement of fish body color for the first time; by comparing Example 3 with Comparative Examples 9 and 10, it can be known that changing the water flow speed multiple times and using water flow stimulation can stimulate the movement frequency of groupers, accelerate the metabolic rate, and prompt the more efficient synthesis and distribution of chromatophores. At the same time, the dynamic water flow can stimulate the body color regulation mechanism of groupers to adapt to the water flow change, prevent the chromatophores from entering an "inert" state due to lack of external stimulation, and thus maintain or strengthen the body color.

[0140] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for cultivating grouper fish to enhance its body color, characterized in that: The following steps are involved: (1) Light induction: using a light source of white, red, blue, and yellow to alternately irradiate; (2) Color enhancer feeding: feeding at the same time as the basic feed, the components of the color enhancer include Camellia chrysantha polysaccharide, Sargassum fusiformis polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidinoacetic acid and sodium butyrate; when the pH value of the aquaculture water is 7.2-7.7, the color enhancer feeding amount is 0.8-1.5% of the fish body weight; when the pH value of the aquaculture water is 7.8-8.2, the color enhancer feeding amount is 0.15-0.35% of the fish body weight; when the pH value of the aquaculture water is 8.3-8.6, the color enhancer feeding amount is 0.50-1.0% of the fish body weight; (3) Water flow stimulation: The water body is maintained at a flow rate of 0.10-0.15 m / s for 2-4 hours, 0.20-0.30 m / s for 1-3 hours, and 0.35-0.40 m / s for 0.5-1.5 hours. The water flow is stopped at other times.

2. The method for cultivating the grouper to enhance its body color according to claim 1, characterized in that: The irradiation time of the white light source in step (1) is the same as the total irradiation time of the red-blue-yellow light sources.

3. The method for cultivating the grouper to enhance its body color according to claim 1, characterized in that: In step (1), the irradiation time of the white light source is 06:00-18:00, the irradiation time of the red light source is 18:00-02:00, the irradiation time of the blue light source is 02:00-04:00, and the irradiation time of the yellow light source is 04:00-06:

00.

4. The method for cultivating the grouper to enhance its body color according to claim 1, characterized in that: In step (1), the irradiation intensity of the white light source is 100-150 lux, the irradiation intensity of the red light source is 500-1000 lux, the irradiation intensity of the blue light source is 300-500 lux, and the irradiation intensity of the yellow light source is 200-300 lux.

5. The method for cultivating grouper fish to enhance its body color according to claim 1, characterized in that: The components of the basic feed in step (2) include, by weight, 30 to 40 parts of white fish meal, 20 to 35 parts of earthworm meal, 10 to 15 parts of corn gluten meal, 7 to 12 parts of soybean meal, 5 to 10 parts of soybean oil, 2 to 7 parts of choline chloride and 1 to 6 parts of vitamin C.

6. The method for cultivating grouper fish to enhance its body color according to claim 1, characterized in that: The feeding amount of the basic feed in step (2) is 3-5% of the fish body weight.

7. The method for cultivating grouper fish to enhance its body color according to claim 1, characterized in that: The components of the color enhancer in step (2) include, by weight, 20 to 30 parts of Camellia chrysantha polysaccharide, 20 to 25 parts of Sargassum fusiformis polysaccharide, 10 to 18 parts of fucoxanthin, 8 to 12 parts of scallop peptide, 4 to 10 parts of silymarin, 1 to 3 parts of guanidinoacetic acid and 1 to 3 parts of sodium butyrate.

8. The method for cultivating grouper fish to enhance its body color according to claim 7, characterized in that: The components of the color enhancer in step (2) include, by weight, 25 parts of Camellia chrysantha polysaccharide, 22 parts of Sargassum fusiformis polysaccharide, 15 parts of fucoxanthin, 10 parts of scallop peptide, 6 parts of silymarin, 2 parts of guanidinoacetic acid and 2 parts of sodium butyrate.

9. The method for cultivating grouper fish to enhance its body color according to claim 1, characterized in that: In step (2), when the pH value of the aquaculture water is 7.2-7.7, the amount of the color enhancer fed is 1.2% of the fish body weight; when the pH value of the aquaculture water is 7.8-8.2, the amount of the color enhancer fed is 0.30% of the fish body weight; when the pH value of the aquaculture water is 8.3-8.6, the amount of the color enhancer fed is 0.80% of the fish body weight.

10. The method for cultivating grouper fish to enhance its body color according to claim 1, characterized in that: In step (3), the water body is maintained at a flow rate of 0.12 m / s for 3 h, at a flow rate of 0.25 m / s for 2 h, and at a flow rate of 0.38 m / s for 1.0 h every day, and the water flow is stopped for the rest of the time.

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