Cordyceps sinensis after-sun repair composition and application thereof

Abstract

The invention provides a cordyceps sinensis after-sun repair composition and application thereof. The cordyceps sinensis after-sun repair composition comprises a cordyceps sinensis extract, tetrahydromethylpyrimidine carboxylic acid and trehalose. The invention creatively finds that the cordyceps sinensis extract, the tetrahydromethylpyrimidine carboxylic acid and the trehalose have a synergistic effect in the aspect of skin after-sun repair, and the after-sun repair composition composed of the cordyceps sinensis extract, the tetrahydromethylpyrimidine carboxylic acid and the trehalose can enhance skin elasticity, improve skin water content, relieve adverse reactions such as red and swollen after sun drying, stabbing pain and the like, rebuild skin barriers and prevent skin aging. The skin health state can be quickly recovered.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a cordyceps sinensis post-sun repair composition and its application. Background Art

[0002] When the skin is exposed to sunlight for a long time, or when sun protection measures are improper or not taken, it will be over-irradiated by ultraviolet rays, and sunburn symptoms such as redness and stinging will appear. In response, various post-sun repair products have emerged on the market.

[0003] CN119454502A discloses a collagen tripeptide complex for post-sun repair, which is composed of the following raw materials in parts by weight: 10-20 parts of collagen tripeptide, 1-5 parts of sodium hyaluronate, 3-8 parts of modified glycerol, 1-3 parts of centella asiatica extract, 1-3 parts of green tea extract, 0.5-2 parts of disodium glycyrrhizinate, 1-3 parts of seaweed extract, 2-5 parts of squalane, 0.5-2 parts of vitamin E, 2-5 parts of vitamin B5, 1-3 parts of allantoin, 0.5-2 parts of lavender extract, 1-3 parts of modified titanium dioxide, 0.5-1.5 parts of thickener, 0.3-1 part of preservative, 1-3 parts of emulsifier, and 8-12 parts of deionized water. The complex can effectively relieve post-sun skin discomfort and accelerate the skin self-repair process.

[0004] CN106420471A discloses a skin care product with both sun protection and post-sun repair functions, which specifically includes the following components: sodium copper chlorophyllin, cytokine complex liposome, sargassum extract, lonicera japonica thunb. extract, fraxinus rhynchophylla extract, dipotassium glycyrrhizinate, shea butter, butanediol, β-glucan, sodium hyaluronate, xanthan gum, trehalose, hydrogenated jojoba oil, squalane, cyclopentasiloxane, cetearyl glucoside, cetearyl alcohol, PEG-100 stearate, tocopheryl acetate, bisabolol, preservative, essence, and deionized water. The skin care product has a mild effect, no skin irritation, and has significant sun protection and post-sun repair and other effects.

[0005] At present, the post-sun repair products on the market are uneven, with complex ingredients, and the interaction of complex ingredients is difficult to control, resulting in further damage to the skin and making it difficult to achieve stable and effective skin barrier reconstruction.

[0006] Cordyceps sinensis is a classic precious Chinese medicinal herb in China. Its active ingredients include cordyceps polysaccharide, cordycepic acid, various amino acids, vitamins, alkaloids, minerals, etc. When applied to cosmetics, it can have effects such as antioxidant, anti-aging, moisturizing and nourishing, repair and regeneration, anti-inflammatory and soothing, whitening and brightening the skin, etc. For the extraction of the active ingredients of Cordyceps sinensis, methods such as heating, alcohol precipitation, microwave, carbon dioxide extraction, etc. are commonly used. However, these methods have disadvantages such as being prone to destroying the active ingredients, having more impurities, incomplete extraction of effective substances, and cumbersome process steps, making it difficult to guarantee the efficacy of the obtained Cordyceps sinensis extract.

[0007] Therefore, it is urgent to improve the content of active ingredients in the Cordyceps sinensis extract and, based on the Cordyceps sinensis extract, prepare a post-sun skin barrier repair product with safe, stable and effective ingredients. Summary of the Invention

[0008] Aiming at the deficiencies of the existing technology, the purpose of the present invention is to provide a Cordyceps sinensis post-sun repair composition and its application. The post-sun repair composition provided by the present invention can effectively enhance the skin elasticity and water content, relieve post-sun adverse reactions, and quickly restore the skin to a healthy state.

[0009] To achieve the purpose of this invention, the following technical solutions are adopted:

[0010] In the first aspect, the present invention provides a Cordyceps sinensis post-sun repair composition, and the Cordyceps sinensis post-sun repair composition includes Cordyceps sinensis extract, ectoine and trehalose.

[0011] Cordyceps sinensis is a dried complex of the stroma of the fungus Cordyceps sinensis parasitizing on the larvae of insects in the family Hepialidae and the larval corpse. The Cordyceps sinensis extract mainly contains crude protein, amino acids, D-mannitol, cordycepin, ergosterol, etc., and can play the effects of tendering, whitening, moisturizing and nourishing the skin. Abundant amino acids can provide nutrition, enhance the vitality of epidermal cells, and promote cell metabolism. Among them, glycine, valine and isoleucine also have anti-radiation effects, can resist sunlight irradiation, and keep the skin fair and tender. D-mannitol can keep the skin moist, making the skin soft, delicate and elastic.

[0012] Ectoine can penetrate deep into the epidermis, repair the skin's water-locking system, enhance the skin's immune protection ability, have an outstanding repair effect on various damages of the skin tissue, improve adverse conditions such as thin, rough and scarred skin, and make the skin restore smoothness and gloss.

[0013] The carboxyl, hydroxyl and other polar groups in trehalose form hydrogen bonds with water molecules to bind a large amount of water. As the extracellular colloidal matrix of the skin, it provides water for the skin and at the same time maintains the balance of cell osmotic pressure, thereby protecting the skin barrier.

[0014] The present invention creatively discovers that cordyceps sinensis extract, ectoine, and trehalose have a synergistic effect in the repair of skin after sun exposure. The three can synergistically enhance skin elasticity, increase skin moisture content, relieve adverse reactions such as redness, swelling, and stinging after sun exposure, rebuild the skin barrier, and quickly restore the skin to a healthy state.

[0015] Preferably, the cordyceps sinensis post-sun repair composition comprises, by weight, 0.01 - 5 parts of cordyceps sinensis extract, 0.1 - 10 parts of ectoine, and 0.5 - 20 parts of trehalose.

[0016] The weight parts of the cordyceps sinensis extract can be 0.01 part, 0.05 part, 0.1 part, 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0017] The weight parts of the ectoine can be 0.1 part, 0.5 part, 1 part, 2 part, 3 part, 4 part, 5 part, 6 part, 7 part, 8 part, 9 part, or 10 parts, etc.

[0018] The weight parts of the trehalose can be 0.5 part, 1 part, 2 parts, 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, or 20 parts, etc.

[0019] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0020] In the present invention, the cordyceps sinensis extract, ectoine, and trehalose have excellent post-sun repair effects under the above specific dosage ratios.

[0021] Preferably, the cordyceps sinensis post-sun repair composition comprises, by weight, 0.1 - 3 parts of cordyceps sinensis extract, 0.5 - 5 parts of ectoine, and 1 - 10 parts of trehalose.

[0022] The weight parts of the cordyceps sinensis extract can be 0.1 part, 0.3 part, 0.5 part, 0.8 part, 1 part, 1.3 part, 1.5 part, 1.8 part, 2 part, 2.3 part, 2.5 part, 2.8 part, or 3 parts, etc.

[0023] The weight parts of the ectoine can be 0.5 part, 1 part, 1.5 part, 2 part, 2.5 part, 3 part, 3.5 part, 4 part, 4.5 part, or 5 parts, etc.

[0024] The weight parts of the trehalose can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, or 10 parts, etc.

[0025] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0026] Furthermore, in the present invention, when Cordyceps sinensis extract, ectoine and trehalose are in the above specific dosage ratios, the after-sun repair effect is more remarkable.

[0027] Preferably, the Cordyceps sinensis extract is prepared by a method comprising the following steps:

[0028] Mix the Cordyceps sinensis raw material and Fe3O4@SiO2 magnetic nanoparticles with or without functional group modification with water for extraction; separate the magnetic nanoparticles by applying an external magnetic field, wash the magnetic nanoparticles, and collect the washing solution to obtain the Cordyceps sinensis extract.

[0029] The present invention breaks through the limitations of the traditional preparation method of Cordyceps sinensis extract, creatively introduces Fe3O4@SiO2 magnetic nanoparticles with or without functional group modification, and can complete the preparation of Cordyceps sinensis extract through rapid directional aggregation and separation by magnetic field. The magnetic nanoparticles have a large adsorption capacity, can realize selective adsorption of active ingredients, and retain the active ingredients and their activities of Cordyceps sinensis to the greatest extent; no organic solvents are used, and the damage to active ingredients is small; the process is simple, can be scaled up for production, and at the same time the magnetic nanoparticles can be recycled and reused, which is green, environmentally friendly and low in cost.

[0030] Preferably, the mass ratio of the Cordyceps sinensis raw material to the Fe3O4@SiO2 magnetic nanoparticles with or without functional group modification is (2 - 5):1.

[0031] The specific point values in (2 - 5) can be 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, 4.8 or 5, etc.

[0032] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0033] In the present invention, when the Cordyceps sinensis raw material and the magnetic nanoparticles are in the above specific ratio, more active ingredients and their activities of the Cordyceps sinensis extract can be retained.

[0034] Preferably, the functional group includes any one or a combination of at least two of hydroxyl, amino or carboxyl.

[0035] In the present invention, the Fe3O4@SiO2 magnetic nanoparticles are magnetic nanoparticles prepared by a conventional method, and their surfaces contain hydroxyl groups, and can be further modified with functional groups such as hydroxyl, amino or carboxyl. The specific modification groups in the present invention endow the magnetic nanoparticles with more excellent selective adsorption properties, a larger adsorption capacity, and can better retain the active ingredients and their activities of Cordyceps sinensis.

[0036] Preferably, the extraction method is ultrasonic extraction.

[0037] Preferably, the temperature of the ultrasonic extraction is 40 - 60 °C, such as 40 °C, 42 °C, 44 °C, 46 °C, 48 °C, 50 °C, 52 °C, 54 °C, 56 °C, 58 °C or 60 °C, etc.; the time of the ultrasonic extraction is 40 - 60 min, such as 40 min, 42 min, 44 min, 46 min, 48 min, 50 min, 52 min, 54 min, 56 min, 58 min or 60 min, etc.; the power of the ultrasonic extraction is 100 - 300 W, such as 100 W, 120 W, 140 W, 160 W, 180 W, 200 W, 220 W, 240 W, 260 W, 280 W or 300 W, etc.

[0038] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0039] The present invention prepares Cordyceps sinensis extract by ultrasonic extraction. Under specific ultrasonic process parameters, it has better extraction effect and can retain more active ingredients and their activities of Cordyceps sinensis extract.

[0040] Preferably, the washing reagent is deionized water.

[0041] The present invention does not use any organic solvents and uses deionized water for extraction and washing, which causes little damage to the active ingredients.

[0042] Preferably, the Cordyceps sinensis post - sun repair composition further includes 0.1 - 15 parts by weight of a sensitization reliever.

[0043] The weight parts of the sensitization reliever can be 0.1 part, 0.5 part, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts or 15 parts, etc.

[0044] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0045] Preferably, the sensitization reliever includes any one or a combination of at least two of Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate or Morus rotundata leaf / stem extract.

[0046] Preferably, the sensitization reliever includes Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate and Morus rotundata leaf / stem extract.

[0047] In the present invention, the Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate, and Memecylon umbellatum leaf / stem extract have a synergistic effect as a sensitization reliever. The three can synergistically enhance skin elasticity and water content, relieve adverse reactions after sun exposure, and rebuild the skin barrier.

[0048] Preferably, the sensitization reliever includes 0.1-3 parts by weight of Piper methysticum leaf / root / stem extract, 0.5-5 parts by weight of Lactobacillus / soybean milk fermentation product filtrate, and 0.5-5 parts by weight of Memecylon umbellatum leaf / stem extract.

[0049] The weight parts of the Piper methysticum leaf / root / stem extract can be 0.1 part, 0.3 part, 0.5 part, 0.8 part, 1 part, 1.3 parts, 1.5 parts, 1.8 parts, 2 parts, 2.5 parts, or 3 parts, etc.

[0050] The weight parts of the Lactobacillus / soybean milk fermentation product filtrate can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0051] The weight parts of the Memecylon umbellatum leaf / stem extract can be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, or 5 parts, etc.

[0052] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0053] In the present invention, when the Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate, and Memecylon umbellatum leaf / stem extract are in the above specific dosage ratios, the effect of enhancing post-sun repair is more significant.

[0054] In the second aspect, the present invention provides the application of the Cordyceps sinensis post-sun repair composition as described in the first aspect in the preparation of cosmetics.

[0055] Preferably, the cosmetics include any one of lotion, emulsion, cream, essence, spray, mask, sunscreen, or skin care and hair care products.

[0056] Preferably, the mass percentage content of the Cordyceps sinensis post-sun repair composition in the cosmetics is 0.1-20%, for example, it can be 0.1%, 0.5%, 1%, 2%, 5%, 8%, 10%, 12%, 15%, 18%, or 20%, etc.

[0057] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0058] Preferably, the cosmetic further comprises any one or a combination of at least two of an antioxidant, a humectant, a solvent, an emulsifier, a thickener, a solubilizer, a pH regulator, a chelating agent, a preservative or a fragrance.

[0059] In a third aspect, the present invention provides a Cordyceps sinensis post-sun repair spray, which comprises, by weight, 0.1-20 parts of the Cordyceps sinensis post-sun repair composition described in the first aspect, 0.1-1 part of an antioxidant, 1-10 parts of a humectant and 60-98 parts of deionized water.

[0060] The weight parts of the Cordyceps sinensis post-sun repair composition can be 0.1 part, 0.5 part, 1 part, 2 parts, 5 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts or 20 parts, etc.

[0061] The weight parts of the antioxidant can be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part or 1 part, etc.

[0062] The weight parts of the humectant can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or 10 parts, etc.

[0063] The weight parts of the deionized water can be 60 parts, 65 parts, 70 parts, 75 parts, 80 parts, 85 parts, 90 parts, 95 parts or 98 parts, etc.

[0064] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0065] The Cordyceps sinensis post-sun repair spray provided by the present invention has good stability and bioavailability, effectively enhances skin elasticity, increases skin water content, relieves adverse reactions such as redness and stinging after sun exposure, rebuilds the skin barrier, and quickly restores the skin to a healthy state.

[0066] Preferably, the antioxidant comprises any one or a combination of at least two of p-hydroxyacetophenone, tocopheryl acetate or retinol palmitate.

[0067] Preferably, the humectant comprises any one or a combination of at least two of glycerol, 1,2-pentanediol, butanediol, 1,2-hexanediol, propylene glycol or sodium hyaluronate.

[0068] In a fourth aspect, the present invention provides a preparation method of the Cordyceps sinensis post-sun repair spray as described in the third aspect, and the preparation method comprises:

[0069] Heat and mix ectoine, trehalose, antioxidant, humectant and deionized water, and then add cordyceps extract and anti-allergy agent after cooling for homogenization to obtain the cordyceps post-sun repair spray.

[0070] Preferably, the mixing method is stirring.

[0071] Preferably, the mixing time is 15 - 25 min, for example, it can be 15 min, 16 min, 17 min, 18 min, 19 min, 20 min, 21 min, 22 min, 23 min, 24 min or 25 min, etc.

[0072] Preferably, the heating process is specifically that the temperature is raised to 75 - 85 °C, for example, it can be 75 °C, 76 °C, 77 °C, 78 °C, 79 °C, 80 °C, 81 °C, 82 °C, 83 °C, 84 °C or 85 °C, etc.

[0073] Preferably, the cooling process is specifically that the temperature is lowered to 35 - 45 °C, for example, it can be 35 °C, 36 °C, 37 °C, 38 °C, 39 °C, 40 °C, 41 °C, 42 °C, 43 °C, 44 °C or 45 °C, etc.

[0074] Preferably, the homogenization time is 5 - 15 min, for example, it can be 5 min, 6 min, 7 min, 8 min, 9 min, 10 min, 11 min, 12 min, 13 min, 14 min or 15 min, etc.

[0075] Other specific point values within the above numerical ranges can be selected and will not be elaborated one by one here.

[0076] Compared with the prior art, the present invention has the following beneficial effects:

[0077] The present invention creatively discovers that cordyceps extract, ectoine and trehalose have a synergistic effect in skin post-sun repair. The post-sun repair composition composed of the three can enhance skin elasticity, increase skin water content, relieve adverse reactions such as post-sun redness and stinging, rebuild the skin barrier, and quickly restore the skin to a healthy state.

[0078] The present invention introduces Fe3O4@SiO2 magnetic nanoparticles with or without functional group modification, and prepares cordyceps extract through rapid magnetic field-directed aggregation and separation, breaking through the limitations of traditional cordyceps extract preparation methods. The magnetic nanoparticles have a large adsorption capacity, can achieve selective adsorption of active ingredients, and retain the active ingredients of cordyceps to the greatest extent. Detailed implementation mode

[0079] To further illustrate the technical means and effects adopted by the present invention, the following further describes the technical solution of the present invention in combination with the preferred embodiments of the present invention. However, the present invention is not limited to the scope of the embodiments.

[0080] For those not specifying specific techniques or conditions in the embodiments, they shall be in accordance with the techniques or conditions described in the literature in this field or in accordance with the product specifications. For those reagents or instruments not indicating the manufacturer, they are all conventional products that can be obtained through regular channels.

[0081] The sources of the materials used in the following specific embodiments are as follows:

[0082] Raw material name Purchasing manufacturer Model Cordyceps sinensis raw material Yichang Shanchengshuidu Cordyceps sinensis Co., Ltd. Cordyceps sinensis Ectoine Bitop AG Ectoin Natural Trehalose Specco SpecKare TRHL02 Piper methysticum leaf / root / stem extract Oxyalis 10％ Lactobacillus / soybean fermentation product filtrate Oxyalis LF16-35 Memecylon umbellatum leaf / stem extract RAHN Myramaze

[0083] Preparation Example 1

[0084] This preparation example provides a cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0085] (1) Preparation of Fe3O4@SiO2-NH2 magnetic nanoparticles: Mix 15 g of ferric chloride hexahydrate, 8.5 g of ferrous chloride tetrahydrate, 12 mL of concentrated hydrochloric acid and 60 mL of deionized water, and stir until completely dissolved; then add 120 mL of ammonia water and 600 mL of deionized water, stir for 25 min, mix evenly to produce black magnetic particles; separate the magnetic particles with a magnet, and wash them alternately with deionized water and methanol 3 times to obtain Fe3O4 magnetic nanoparticles.

[0086] Disperse 1.5 g of Fe3O4 magnetic nanoparticles by ultrasonic wave with deionized water, add 100 mL of absolute ethanol, 5 mL of 25% ammonia water and 3 mL of tetraethyl orthosilicate, stir overnight at 35 °C; centrifuge to collect the precipitate, and wash it alternately with deionized water and absolute ethanol 3 times until the supernatant is clear to obtain Fe3O4@SiO2 magnetic nanoparticles.

[0087] Disperse 500 mg of Fe3O4@SiO2 magnetic nanoparticles by ultrasonic wave with 80 mL of toluene, add 10 mL of 3-aminopropyltriethoxysilane (APTES), reflux at 120 °C for 15 h; cool to room temperature, separate with a magnet, and wash with absolute ethanol 3 times to obtain Fe3O4@SiO2-NH2 magnetic nanoparticles.

[0088] (2) Dry, crush and sieve (90 mesh) the cordyceps sinensis raw materials, take 3 g of the sieved cordyceps sinensis raw materials, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of deionized water, mix them, and extract by ultrasonic wave (power 200 W) at 50 °C for 50 min; separate the magnetic nanoparticles by applying an external magnetic field, wash the magnetic nanoparticles with deionized water, and collect the washing solution to obtain the cordyceps sinensis extract.

[0089] Preparation Example 2

[0090] This preparation example provides a cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0091] (1) Preparation of Fe3O4@SiO2-COOH magnetic nanoparticles: Take 1.5 g of the Fe3O4@SiO2-NH2 magnetic nanoparticles prepared in Preparation Example 1 and disperse them by ultrasonic wave with 200 mL of N,N-dimethylformamide; dissolve 3 g of succinic anhydride in 50 mL of N,N-dimethylformamide, and slowly add the succinic anhydride solution dropwise to the reaction system of the magnetic nanoparticles under stirring. Stir and react at 35 °C and 150 rpm for 18 h; separate with a magnet and wash 3 times with absolute ethanol to obtain Fe3O4@SiO2-COOH magnetic nanoparticles.

[0092] (2) Dry, crush and sieve (100 mesh) the cordyceps sinensis raw materials, take 2 g of the sieved cordyceps sinensis raw materials, 1 g of Fe3O4@SiO2-COOH magnetic nanoparticles and 60 mL of deionized water, mix them, and extract for 60 min by ultrasonic wave (power 100 W) at 40 °C; separate the magnetic nanoparticles by applying an external magnetic field, wash the magnetic nanoparticles with deionized water, and collect the washing liquid to obtain the cordyceps sinensis extract.

[0093] Preparation Example 3

[0094] This preparation example provides a cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0095] (1) Preparation of Fe3O4@SiO2 magnetic nanoparticles: Mix 0.7 g of ferric chloride hexahydrate, 1.8 g of sodium acetate and 25 mL of absolute ethanol, then add 0.5 mL of diethylene glycol monoethyl ether and 0.5 mL of deionized water, stir for 30 min to mix evenly, and react overnight at 200 °C; centrifuge to collect the precipitate, and wash it alternately with deionized water and absolute ethanol for 3 times until the supernatant is clear to obtain Fe3O4 magnetic nanoparticles. Disperse 2 g of Fe3O4 magnetic nanoparticles by ultrasonic wave with deionized water, add 150 mL of absolute ethanol, 5 mL of 25% ammonia water and 3 mL of tetraethyl orthosilicate, and stir overnight at 30 °C; centrifuge to collect the precipitate, and wash it alternately with deionized water and absolute ethanol for 3 times until the supernatant is clear to obtain Fe3O4@SiO2 magnetic nanoparticles.

[0096] (2) Drying, crushing and sieving the Cordyceps sinensis raw material (80 mesh), taking 5 g of the sieved Cordyceps sinensis raw material, 1 g of Fe3O4@SiO2 magnetic nanoparticles and 60 mL of deionized water, mixing, and extracting at 60°C ultrasonically (power 300 W) for 40 min; separating the magnetic nanoparticles by applying an external magnetic field, washing the magnetic nanoparticles with deionized water, collecting the washing liquid, and obtaining the Cordyceps sinensis extract.

[0097] Preparation Example 4

[0098] This preparation example provides a Cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0099] (1) Preparation of Fe3O4@SiO2-SH magnetic nanoparticles: 500 mg of Fe3O4@SiO2 magnetic nanoparticles prepared in Preparation Example 1 were ultrasonically dispersed with 150 mL of propylene glycol. Under nitrogen protection and stirring, the temperature was raised to 80°C, and 3 mL of aqueous ammonia, 3 mL of mercaptopropyltrimethoxysilane and 80 mL of methanol were added to react for 2 h. Stirring was continued at 80°C for 6 h. The reaction product was separated with a magnet and washed alternately with deionized water and methanol for 3 times until the pH was neutral to obtain Fe3O4@SiO2-SH magnetic nanoparticles.

[0100] (2) The only difference from Preparation Example 1 is that the "Fe3O4@SiO2-NH2 magnetic nanoparticles" are replaced with an equal amount of "Fe3O4@SiO2-SH magnetic nanoparticles", and the other raw materials and steps remain unchanged.

[0101] Preparation Example 5

[0102] This preparation example provides a Cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0103] (1) Preparation of Fe3O4@PSA magnetic nanoparticles: Take 2 g of the Fe3O4 magnetic nanoparticles prepared in Preparation Example 1, mix thoroughly with 1 g of ethylenediamine-N-propylsilane (PSA), add 20 mL of anhydrous ethanol, vortex for 2 min, and mix evenly; ultrasonicate at 1000 W power for 1 h, let stand for 1 h, centrifuge at 3000 rpm for 5 min, discard the supernatant, place the product under vacuum drying at 60°C for 4 h, take it out and pass it through a 150 μm sieve to obtain Fe3O4@PSA magnetic nanoparticles.

[0104] (2) The only difference from Preparation Example 1 is that the "Fe3O4@SiO2-NH2 magnetic nanoparticles" are replaced with an equal amount of "Fe3O4@PSA magnetic nanoparticles", and the other raw materials and steps remain unchanged.

[0105] Preparation Example 6

[0106] This Preparation Example provides a cordyceps sinensis extract, which is only different from Preparation Example 1 in that: "3 g of sieved cordyceps sinensis raw material" is replaced with "1.67 g of sieved cordyceps sinensis raw material", and other raw materials and steps remain unchanged.

[0107] Preparation Example 7

[0108] This Preparation Example provides a cordyceps sinensis extract, which is only different from Preparation Example 1 in that: "3 g of sieved cordyceps sinensis raw material" is replaced with "6 g of sieved cordyceps sinensis raw material", and other raw materials and steps remain unchanged.

[0109] Preparation Example 8

[0110] This Preparation Example provides a cordyceps sinensis extract, which is only different from Preparation Example 1 in that: "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of deionized water" is replaced with "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of deep eutectic solvent", and other raw materials and steps remain unchanged;

[0111] Preparation of the deep eutectic solvent: Weigh the corresponding choline chloride and glycerol (molar ratio 1:2) respectively, place them under constant temperature at 80 °C and continuously stir at a speed of 600 r / min. After 2 h, cool to room temperature to obtain a clear and transparent deep eutectic solvent.

[0112] Preparation Example 9

[0113] This Preparation Example provides a cordyceps sinensis extract, which is only different from Preparation Example 1 in that: "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of deionized water" is replaced with "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of 70% ethanol aqueous solution", and other raw materials and steps remain unchanged.

[0114] Preparation Example 10

[0115] This Preparation Example provides a cordyceps sinensis extract, which is only different from Preparation Example 1 in that: "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of deionized water" is replaced with "mixing 3 g of sieved cordyceps sinensis raw material, 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and 60 mL of 20% methanol aqueous solution", and other raw materials and steps remain unchanged.

[0116] Preparation Example 11

[0117] This Preparation Example provides a Cordyceps sinensis extract, which is prepared by a method comprising the following steps:

[0118] (1) Preparation of Fe3O4@SiO2-NH2 magnetic nanoparticles: Refer to Preparation Example 1.

[0119] (2) Dry, crush, and sieve (90 mesh) the Cordyceps sinensis raw material. Take 3 g of the sieved Cordyceps sinensis raw material and mix it with 60 mL of a 20% methanol aqueous solution. Extract at 50 °C by ultrasonic treatment (power 200 W) for 50 min; then add 1 g of Fe3O4@SiO2-NH2 magnetic nanoparticles and incubate at 50 °C for 50 min; separate the magnetic nanoparticles by applying an external magnetic field, wash the magnetic nanoparticles with deionized water, and collect the washing solution to obtain the Cordyceps sinensis extract.

[0120] Preparation Example 12

[0121] This Preparation Example provides a Cordyceps sinensis extract, which differs from Preparation Example 1 only in that "washing the magnetic nanoparticles with deionized water" is replaced by "washing the magnetic nanoparticles with methanol", and other raw materials and steps remain unchanged.

[0122] Preparation Example 13

[0123] This Preparation Example provides a Cordyceps sinensis extract, which differs from Preparation Example 1 only in that "extract at 50 °C by ultrasonic treatment (power 200 W) for 50 min" is replaced by "extract at 70 °C by ultrasonic treatment (power 350 W) for 30 min", and other raw materials and steps remain unchanged.

[0124] Preparation Example 14

[0125] This Preparation Example provides a Cordyceps sinensis extract, which differs from Preparation Example 1 only in that "extract at 50 °C by ultrasonic treatment (power 200 W) for 50 min" is replaced by "extract at 30 °C by ultrasonic treatment (power 50 W) for 70 min", and other raw materials and steps remain unchanged.

[0126] Preparation Example 15

[0127] This Preparation Example provides a Cordyceps sinensis extract, which differs from Preparation Example 1 only in that "extract at 50 °C by ultrasonic treatment (power 200 W) for 50 min" is replaced by "place it in a pressure-resistant cavity, seal the cavity, fill it with nitrogen until the pressure in the cavity reaches 400 kPa, then close the nitrogen, heat the cavity, raise its temperature to 120 °C within 10 min, then raise the temperature to 200 °C at a rate of 4 °C / min and maintain for 10 min, and after completion, let the cavity cool naturally to room temperature", and other raw materials and steps remain unchanged.

[0128] Example 1

[0129] This embodiment provides a post-sun repair spray for Cordyceps sinensis, which includes the following components by weight:

[0130]

[0131] The preparation method is as follows:

[0132] Heat ectoine, trehalose, antioxidant, humectant and deionized water, stir at 80 °C for 20 min, cool down to 40 °C, then add Cordyceps sinensis extract and anti-allergy agent and homogenize for 10 min to obtain the post-sun repair spray for Cordyceps sinensis.

[0133] Example 2

[0134] This embodiment provides a post-sun repair spray for Cordyceps sinensis, which includes the following components by weight:

[0135]

[0136] The preparation method is as follows:

[0137] Heat ectoine, trehalose, antioxidant, humectant and deionized water, stir at 75 °C for 25 min, cool down to 35 °C, then add Cordyceps sinensis extract and anti-allergy agent and homogenize for 15 min to obtain the post-sun repair spray for Cordyceps sinensis.

[0138] Example 3

[0139] This embodiment provides a post-sun repair spray for Cordyceps sinensis, which includes the following components by weight:

[0140]

[0141] The preparation method is as follows:

[0142] Heat ectoine, trehalose, antioxidant, humectant and deionized water, stir at 85 °C for 15 min, cool down to 45 °C, then add Cordyceps sinensis extract and anti-allergy agent and homogenize for 5 min to obtain the post-sun repair spray for Cordyceps sinensis.

[0143] Examples 4 - 15

[0144] This embodiment provides a post-sun repair spray for Cordyceps sinensis. The difference from Example 1 is only that "the Cordyceps sinensis extract prepared in Preparation Example 1" is replaced with an equal amount of "the Cordyceps sinensis extract prepared in Preparation Examples 4 - 15", and other raw materials and steps remain unchanged.

[0145] Example 16

[0146] This embodiment provides a post-sun repair spray for Cordyceps sinensis, and its difference from Embodiment 1 is only that: the Piper methysticum leaf / root / stem extract is not added, and the reduced amount is proportionally distributed to the filtrate of Lactobacillus / soybean milk fermentation products and the Morus rotundata leaf / stem extract, and other raw materials and steps remain unchanged.

[0147] Embodiment 17

[0148] This embodiment provides a post-sun repair spray for Cordyceps sinensis, and its difference from Embodiment 1 is only that: the filtrate of Lactobacillus / soybean milk fermentation products is not added, and the reduced amount is proportionally distributed to the Piper methysticum leaf / root / stem extract and the Morus rotundata leaf / stem extract, and other raw materials and steps remain unchanged.

[0149] Embodiment 18

[0150] This embodiment provides a post-sun repair spray for Cordyceps sinensis, and its difference from Embodiment 1 is only that: the Morus rotundata leaf / stem extract is not added, and the reduced amount is proportionally distributed to the Piper methysticum leaf / root / stem extract and the filtrate of Lactobacillus / soybean milk fermentation products, and other raw materials and steps remain unchanged.

[0151] Comparative Example 1

[0152] This comparative example provides a post-sun repair spray, and its difference from Embodiment 1 is only that: the Cordyceps sinensis extract is not added, and the reduced amount is proportionally distributed to ectoine and trehalose, and other raw materials and steps remain unchanged.

[0153] Comparative Example 2

[0154] This comparative example provides a post-sun repair spray for Cordyceps sinensis, and its difference from Embodiment 1 is only that: ectoine is not added, and the reduced amount is proportionally distributed to the Cordyceps sinensis extract and trehalose, and other raw materials and steps remain unchanged.

[0155] Comparative Example 3

[0156] This comparative example provides a post-sun repair spray for Cordyceps sinensis, and its difference from Embodiment 1 is only that: trehalose is not added, and the reduced amount is proportionally distributed to the Cordyceps sinensis extract and ectoine, and other raw materials and steps remain unchanged.

[0157] Test Example 1

[0158] Healthy volunteers were selected, randomly including both men and women, aged 20 - 48 years old, with no acute facial inflammation, not using hormonal drugs, and not applying topical preparations; they were randomly assigned, with 15 people in each group. During the period from 10:00 to 12:00 in August when there was sufficient sunlight, they carried out outdoor activities for 2 hours and their facial skin was irradiated by sunlight. After sun exposure, the facial skin of the volunteers all showed varying degrees of redness, stinging, and burning sensations. Immediately, the post - sun repair sprays prepared in Examples 1 - 18 and Comparative Examples 1 - 3 were used 3 times a day for 7 consecutive days, and the blank control group did not use any product; before and after product use, the skin moisture content was measured using a skin moisture tester (SKIN DIAGNOSTICS D202).

[0159] The test results are shown in Table 1. The composition provided by the present invention has excellent post - sun repair effects. Cordyceps sinensis extract, ectoine, and trehalose have a synergistic effect in post - sun skin repair. The three can synergistically increase the skin water content, rebuild the skin barrier, and quickly restore the healthy state of the skin; Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate, and Memecylon umbellatum leaf / stem extract have a synergistic effect as anti - inflammatory agents, and the three can synergistically enhance the post - sun repair efficacy. The specific preparation method of Cordyceps sinensis extract in the present invention can retain the active ingredients of Cordyceps sinensis to the greatest extent and improve the post - sun repair efficacy of the composition; the type of magnetic nanoparticles, the modification of their functional groups, the ratio of raw materials to magnetic nanoparticles, the extraction reagents, the washing reagents, and the ultrasonic process parameters will all affect the retention of the active ingredients of Cordyceps sinensis extract, and thus affect the post - sun repair efficacy of the composition.

[0160] Table 1

[0161]

[0162]

[0163] Test Example 2

[0164] Healthy volunteers were selected, randomly including both men and women, aged 20 - 48 years old, with no acute facial inflammation, not using hormonal drugs, and not applying topical preparations; they were randomly assigned, with 15 people in each group. The inner side of the volunteers' arms was sunburned using an ultraviolet lamp to form erythema; the post - sun repair sprays prepared in Examples 1 - 18 and Comparative Examples 1 - 3 were used 2 times a day for 5 consecutive days, and the blank control group did not use any product; before and after product use, the erythema index was measured using a skin erythema meter (Mexameter MX18).

[0165] The test results are shown in Table 2. The composition provided by the present invention has excellent post-sun repair effects. Cordyceps sinensis extract, ectoine, and trehalose have a synergistic effect in post-sun skin repair. The three can synergistically relieve adverse reactions such as redness, swelling, and stinging after sun exposure, rebuild the skin barrier, and quickly restore the skin to a healthy state; Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate, and Memecylon umbellatum leaf / stem extract have a synergistic effect as a sensitization reliever, and the three can synergistically enhance the post-sun repair effect. The specific preparation method of Cordyceps sinensis extract of the present invention can retain the active ingredients and their activities of Cordyceps sinensis to the greatest extent, and improve the post-sun repair effect of the composition; the type of magnetic nanoparticles, the modification of their functional groups, the ratio of raw materials to magnetic nanoparticles, the extraction reagent, the washing reagent, and the ultrasonic process parameters will all affect the retention of the active ingredients of Cordyceps sinensis extract, and thus affect the post-sun repair effect of the composition.

[0166] Table 2

[0167] Test sample Change in erythema index before and after use (%) Example 1 -35.33 Example 2 -34.20 Example 3 -33.60 Example 4 -17.55 Example 5 -16.71 Example 6 -16.38 Example 7 -15.42 Example 8 -15.31 Example 9 -14.19 Example 10 -12.47 Example 11 -10.03 Example 12 -18.55 Example 13 -18.26 Example 14 -17.09 Example 15 -10.38 Example 16 -25.20 Example 17 -26.21 Example 18 -24.73 Control Example 1 -6.47 Control Example 2 -7.02 Control Example 3 -5.83 Blank control +8.25

[0168] Test Example 3

[0169] Healthy volunteers were selected, randomly divided into men and women, aged 20 - 48 years old, with no acute inflammation on the face, not using hormonal drugs, and not applying topical preparations; they were randomly assigned, with 15 people in each group. During the period from 10:00 to 12:00 in August when there was sufficient sunlight, they carried out outdoor activities for 2 hours, and the facial skin was irradiated by sunlight. After sun exposure, the facial skin of the volunteers showed varying degrees of redness, swelling, stinging, and burning sensations. Immediately, the post-sun repair sprays prepared in Examples 1 - 18 and Comparative Examples 1 - 3 were used 2 times a day for 14 consecutive days. The blank control group did not use any products; the skin elasticity values were measured with a skin elasticity tester (Cutometer DualMPA580) before and after product use.

[0170] The test results are shown in Table 3. The composition provided by the present invention has excellent post-sun repair effects. Cordyceps sinensis extract, ectoine, and trehalose have a synergistic effect in post-sun skin repair. The three can synergistically enhance skin elasticity, rebuild the skin barrier, and quickly restore the skin to a healthy state; Piper methysticum leaf / root / stem extract, Lactobacillus / soybean milk fermentation product filtrate, and Memecylon umbellatum leaf / stem extract have a synergistic effect as a sensitization reliever, and the three can synergistically enhance the post-sun repair effect. The specific preparation method of Cordyceps sinensis extract of the present invention can retain the active ingredients and their activities of Cordyceps sinensis to the greatest extent, and improve the post-sun repair effect of the composition; the type of magnetic nanoparticles, the modification of their functional groups, the ratio of raw materials to magnetic nanoparticles, the extraction reagent, the washing reagent, and the ultrasonic process parameters will all affect the retention of the active ingredients of Cordyceps sinensis extract, and thus affect the post-sun repair effect of the composition.

[0171] Table 3

[0172] Test sample Change in skin elasticity value before and after use (%) Example 1 +28.83 Example 2 +28.25 Example 3 +27.70 Example 4 +14.68 Example 5 +14.20 Example 6 +13.19 Example 7 +12.77 Example 8 +12.13 Example 9 +10.60 Example 10 +10.24 Example 11 +8.72 Example 12 +9.01 Example 13 +8.45 Example 14 +8.26 Example 15 +7.80 Example 16 +21.75 Example 17 +20.20 Example 18 +20.98 Control Example 1 +2.02 Control Example 2 +1.57 Control Example 3 +1.66 Blank control -4.13

[0173] The present invention illustrates a post - sun repair composition of Cordyceps sinensis and its application through the above - mentioned embodiments. However, the present invention is not limited to the above - mentioned embodiments, that is, it does not mean that the present invention must rely on the above - mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, the equivalent substitution of each raw material of the product of the present invention, the addition of auxiliary components, the selection of specific methods, etc., all fall within the protection scope and the disclosure scope of the present invention.

[0174] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above - mentioned embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0175] In addition, it should be noted that, in the case of no contradiction, the various specific technical features described in the above - mentioned specific embodiments can be combined in any appropriate way. To avoid unnecessary repetition, the present invention will not separately describe various possible combination methods.

Claims

1. A Cordyceps sinensis after-sun repair composition, characterized in that: The cordyceps after-sun care composition comprises cordyceps extract, ectoine and trehalose.

2. The Cordyceps sinensis after-sun care composition according to claim 1, characterized in that: The cordyceps after-sun care composition comprises, by weight, 0.01-5 parts of cordyceps extract, 0.1-10 parts of ectoine and 0.5-20 parts of trehalose; Preferably, the cordyceps after-sun care composition comprises, by weight, 0.1-3 parts of cordyceps extract, 0.5-5 parts of ectoine and 1-10 parts of trehalose.

3. The Cordyceps sinensis after-sun repair composition according to claim 1 or 2, characterized in that: The Cordyceps sinensis extract is prepared by a method comprising the following steps: The cordyceps sinensis raw material and the functional group-modified or unmodified Fe3O4@SiO2 magnetic nanoparticles are mixed with water for extraction; the magnetic nanoparticles are separated by an external magnetic field, the magnetic nanoparticles are washed, and the washing liquid is collected to obtain the cordyceps sinensis extract; Preferably, the mass ratio of the Cordyceps sinensis raw material and the functional group-modified or unmodified Fe3O4@SiO2 magnetic nanoparticles is (2-5):1; Preferably, the functional group includes any one or a combination of at least two of hydroxyl, amino or carboxyl groups; Preferably, the extraction method is ultrasonic extraction; Preferably, the ultrasonic temperature is 40-60°C, the ultrasonic time is 40-60min, and the ultrasonic power is 100-300W; Preferably, the washing agent is deionized water.

4. The Cordyceps sinensis after-sun care composition according to any one of claims 1 to 3, characterized in that: The cordyceps after-sun repair composition further comprises 0.1-15 parts of a soothing agent by weight; Preferably, the allergy-relief agent comprises any one of Kava Piper melongena leaf / root / stem extract, Lactobacillus / soy milk fermentation product filtrate or Milo leaf / stem extract, or a combination of at least two thereof; Preferably, the allergy-relief agent comprises Kava Piper melongena leaf / root / stem extract, Lactobacillus / soy milk fermentation product filtrate and Milotoxin leaf / stem extract; Preferably, the allergy-relief agent comprises, by weight, 0.1-3 parts of Piper methysticum leaf / root / stem extract, 0.5-5 parts of lactobacillus / soy milk fermentation product filtrate, and 0.5-5 parts of Miróu leaf / stem extract.

5. Use of the Cordyceps sinensis after-sun repair composition according to any one of claims 1 to 4 in the preparation of cosmetics.

6. The use according to claim 5, characterized in that: The cosmetics include any one of lotion, emulsion, cream, essence, spray, mask, sunscreen or cleaning products; Preferably, the mass percentage of the cordyceps after-sun repair composition in the cosmetic is 0.1-20%; Preferably, the cosmetic further comprises any one or a combination of at least two of an antioxidant, a moisturizer, a solvent, an emulsifier, a thickener, a solubilizer, a pH adjuster, a chelating agent, a preservative or a fragrance.

7. A Cordyceps sinensis after-sun repair spray, characterized in that: The cordyceps after-sun repair spray comprises, by weight, 0.1-20 parts of the cordyceps after-sun repair composition according to any one of claims 1 to 4, 0.1-1 part of an antioxidant, 1-10 parts of a moisturizer and 60-98 parts of deionized water.

8. The cordyceps after-sun repair spray according to claim 7, characterized in that: The antioxidant comprises any one or a combination of at least two of p-hydroxyacetophenone, tocopherol acetate or retinol palmitate; Preferably, the moisturizing agent includes any one of glycerin, 1,2-pentanediol, butylene glycol, 1,2-hexanediol, propylene glycol or sodium hyaluronate, or a combination of at least two thereof.

9. The method for preparing the Cordyceps sinensis after-sun repair spray according to claim 7 or 8, characterized in that: The preparation method comprises: The ectohydromethylpyrimidine carboxylic acid, trehalose, antioxidant, moisturizer and deionized water are heated and mixed, and after cooling, the cordyceps sinensis extract and the allergy-relieving agent are added and homogenized to obtain the cordyceps sinensis after-sun repair spray.

10. The preparation method according to claim 9, characterized in that: The mixing method is stirring; Preferably, the mixing time is 15-25min; Preferably, the heating process is specifically to increase the temperature to 75-85°C; Preferably, the cooling process is specifically to reduce the temperature to 35-45°C; Preferably, the homogenization time is 5-15 min.

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