Pharmaceutical composition for treating diarrhea of yak calves and preparation method of pharmaceutical composition

Abstract

The invention discloses a pharmaceutical composition for treating diarrhea of yak calves, and relates to the technical field of biological pharmacy, the pharmaceutical composition is prepared from the following raw materials: coptis chinensis, cortex phellodendri, cortex fraxini, Chinese pulsatilla root, purslane, Chinese yam, poria cocos, bighead atractylodes rhizome and liquorice, various natural traditional Chinese medicinal materials are adopted as the raw materials, and the pharmaceutical composition is safe and free of side effects; and adverse effects on the meat quality of the yak calves and the quality of dairy products are avoided. The invention also discloses a preparation method of the pharmaceutical composition for treating diarrhea of yak calves. The preparation method comprises the following steps: step 1, weighing raw materials; 2, pretreating the raw materials; step 3, adding water for soaking; step 4, first heating reflux extraction; step 5, filtering for the first time; step 6, adding water for the second time and extracting; step 7, filtering for the second time; step 8, concentration treatment; the preparation method is easy to operate, effective components in the raw materials can be extracted to the maximum extent through reasonable extraction and concentration processes, and the curative effect of the pharmaceutical composition is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biopharmaceuticals, and specifically to a pharmaceutical composition for treating diarrhea in calves and its preparation method. Background Art

[0002] Diarrhea in calves is one of the common and severely harmful diseases in yak breeding. Due to the incomplete development of their own immune systems and weak resistance, calves are prone to diarrhea under the influence of various factors. The causes of diarrhea in calves are relatively complex, including bacterial infections (such as Escherichia coli, Salmonella, etc.), viral infections (such as rotavirus, coronavirus, etc.), parasitic infections (such as coccidia, nematodes, etc.), and improper feeding management (such as poor feed quality, sudden feed change, unclean drinking water, etc.).

[0003] The invention with the publication number CN115554364A discloses a traditional Chinese and Tibetan medicine composition for preventing and treating diarrhea in yaks. The traditional Chinese and Tibetan medicine composition for preventing and treating diarrhea in yaks includes the following raw material components: 18 parts of Sanguisorba officinalis, 18 parts of Taraxacum mongolicum, 18 parts of Polygonum lapathifolium, 18 parts of Picrorhiza scrophulariiflora, 18 parts of Geranium wilfordii, 21 parts of Inula racemosa, 50 parts of Gymnadenia conopsea, 50 parts of Rheum palmatum, 50 parts of Gentiana scabra, 50 parts of Lithospermum erythrorhizon, and the balance is pure water; the preparation method of the traditional Chinese and Tibetan medicine composition for preventing and treating diarrhea in yaks includes the following steps: (1) pretreatment; (2) classification treatment; (3) extraction treatment; (4) decoction treatment; (5) liquid medicine mixing.

[0004] As shown in the above invention, the existing traditional Chinese and Tibetan medicine compositions for preventing and treating diarrhea in yaks are generally prepared by combining traditional Chinese medicine with Tibetan medicine through processing, and their effects complement each other, being able to produce good synergistic effects. However, some of these traditional Chinese and Tibetan medicinal materials may have relatively limited sources and are difficult to obtain, which may lead to unstable raw material supply and is not conducive to large-scale promotion and application. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the present invention provides a pharmaceutical composition for treating diarrhea in calves and its preparation method, solving the above existing problems.

[0006] To achieve the above objectives, the present invention is realized through the following technical solutions: A pharmaceutical composition for treating diarrhea in calves is made from the following raw materials in parts by weight: 10 - 20 parts of Coptis chinensis, 10 - 20 parts of Phellodendron amurense, 10 - 20 parts of Cortex Fraxini, 10 - 20 parts of Pulsatilla chinensis, 10 - 20 parts of Portulaca oleracea, 15 - 30 parts of Dioscorea opposita, 15 - 30 parts of Poria cocos, 10 - 20 parts of Atractylodes macrocephala, and 5 - 15 parts of Glycyrrhiza uralensis.

[0007] The present invention also discloses a preparation method of a pharmaceutical composition for treating diarrhea in calves, including the following steps:

[0008] Step 1. Raw material weighing: Weigh the raw materials of Coptis chinensis, Phellodendron amurense, Cortex Fraxini, Pulsatilla chinensis, Portulaca oleracea, Dioscorea opposita, Poria cocos, Atractylodes macrocephala, and Glycyrrhiza uralensis according to the above weight portions;

[0009] Step 2. Raw material pretreatment: Wash the weighed raw materials respectively, remove impurities, then cut them into small pieces or sections, and put them into the extraction tank;

[0010] Step 3. Water soaking: Add water 5 to 8 times the total weight of the raw materials to the extraction tank, soak for 30 - 60 minutes, so that the raw materials fully absorb water and expand, and prepare for the subsequent extraction of active ingredients;

[0011] Step 4. First heating reflux extraction: Adopt the heating reflux extraction method, heat the extraction tank to boiling, and keep it in a slightly boiling state for extraction for 1 - 2 hours, so that the active ingredients in the raw materials are fully dissolved into the water;

[0012] Step 5. First filtration: After the extraction is completed, filter the extraction liquid while it is hot, remove the medicinal residues, and obtain the first extraction liquid, initially separating the liquid containing active ingredients;

[0013] Step 6. Second water addition and extraction: Add water 3 to 5 times the total weight of the raw materials to the medicinal residues, and perform heating reflux extraction again. Similarly, heat to boiling and then keep it in a slightly boiling state for extraction for 1 - 2 hours to further extract the residual active ingredients in the medicinal residues;

[0014] Step 7. Second filtration: After the second extraction is completed, filter the extraction liquid while it is hot to obtain the second extraction liquid, and complete the separation of the second extraction liquid of active ingredients;

[0015] Step 8. Concentration treatment: Combine the first extraction liquid and the second extraction liquid, put them into the concentration device, and perform reduced pressure concentration at a temperature of 60 - 80 °C until the volume of the concentrated liquid is 1 / 4 - 1 / 5 of the total volume of the original extraction liquid, so as to increase the concentration of active ingredients;

[0016] Step 9. Adding preservatives and preparing finished products: Cool the concentrated liquid to room temperature, add an appropriate amount of preservatives, and stir evenly to obtain a pharmaceutical composition for treating diarrhea in calves.

[0017] Preferably, the preservative is sodium benzoate, and the addition amount is 0.1% - 0.2% of the weight of the concentrated liquid.

[0018] Preferably, the weight portion ratio of Coptis chinensis, Phellodendron amurense, Cortex Fraxini, Pulsatilla chinensis, Portulaca oleracea, Dioscorea opposita, Poria cocos, Atractylodes macrocephala, and Glycyrrhiza uralensis is: Coptis chinensis 12 - 18 parts, Phellodendron amurense 12 - 8 parts, Cortex Fraxini 12 - 18 parts, Pulsatilla chinensis 12 - 18 parts, Portulaca oleracea 12 - 18 parts, Dioscorea opposita 18 - 25 parts, Poria cocos 18 - 25 parts, Atractylodes macrocephala 12 - 18 parts, Glycyrrhiza uralensis 8 - 12 parts.

[0019] Preferably, during the heating and reflux extraction process, the pressure inside the extraction tank is maintained at 0.1 - 0.3 MPa to promote the dissolution of active ingredients in the raw materials.

[0020] Preferably, the pharmaceutical composition can be made into an oral liquid dosage form. The oral liquid dosage form of the pharmaceutical composition further includes a flavoring agent, and the flavoring agent is sucrose or honey, and the added amount is 5% - 10% of the weight of the concentrated solution.

[0021] Preferably, the pharmaceutical composition can be made into a granule dosage form. When preparing the granules, a filler and a binder are added to the concentrated solution. The filler is starch or dextrin, and the added amount is 10% - 20% of the weight of the concentrated solution. The binder is polyvinylpyrrolidone or hydroxypropyl methylcellulose, and the added amount is 1% - 3% of the weight of the concentrated solution.

[0022] Preferably, the application of the pharmaceutical composition for treating diarrhea in calves in the preparation of a feed additive for preventing diarrhea in calves: adding the pharmaceutical composition to the feed of calves at a ratio of 0.5% - 2% to enhance the spleen and stomach function of calves and improve the body's resistance to diarrhea.

[0023] The present invention provides a pharmaceutical composition for treating diarrhea in calves and a preparation method. Compared with the prior art, it has the following beneficial effects:

[0024] 1. The pharmaceutical composition for treating diarrhea in calves uses a variety of natural Chinese medicinal materials as raw materials, is safe and has no side effects, will not have an adverse impact on the meat quality and dairy product quality of calves, meets the requirements of green farming and food safety. The raw materials in the pharmaceutical composition are formulated and interact synergistically, which can not only effectively inhibit and kill pathogens such as bacteria, viruses, and parasites causing diarrhea, but also regulate the spleen and stomach function of calves, enhance the body's resistance, fundamentally treat diarrhea in calves, and is not easy to relapse.

[0025] 2. The preparation method of the pharmaceutical composition for treating diarrhea in calves is easy to operate. Through reasonable extraction and concentration processes, it can maximize the extraction of active ingredients in the raw materials, improve the curative effect of the pharmaceutical composition. The generated pharmaceutical composition can be made into different dosage forms according to the actual situation, such as oral liquid, granules, etc., which is convenient for administration and improves the medication compliance of calves. Description of the Drawings

[0026] Figure 1 It is a schematic flow chart of the method of the present invention. Detailed Embodiments

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts shall fall within the protection scope of the present invention.

[0028] Refer to Figure 1 , the present invention provides the following three technical solutions (: (There are five technical solutions in the specification)

[0029] The first embodiment: A pharmaceutical composition for treating diarrhea in calves is prepared from the following raw materials in parts by weight: 10-20 parts of Coptis chinensis, 10-20 parts of Phellodendron amurense, 10-20 parts of Cortex Fraxini, 10-20 parts of Pulsatilla chinensis, 10-20 parts of Portulaca oleracea, 15-30 parts of Chinese yam, 15-30 parts of Poria cocos, 10-20 parts of Atractylodes macrocephala, and 5-15 parts of Glycyrrhiza uralensis.

[0030] The second embodiment: A preparation method of a pharmaceutical composition for treating diarrhea in calves, comprising the following steps:

[0031] Step 1, raw material weighing: Weigh 10 parts of Coptis chinensis, 10 parts of Phellodendron amurense, 10 parts of Cortex Fraxini, 10 parts of Pulsatilla chinensis, 10 parts of Portulaca oleracea, 15 parts of Chinese yam, 15 parts of Poria cocos, 10 parts of Atractylodes macrocephala, and 5 parts of Glycyrrhiza uralensis;

[0032] Step 2, raw material pretreatment: Wash the weighed raw materials respectively, remove impurities, then cut them into small pieces or sections, and put them into an extraction tank;

[0033] Step 3, water soaking: Add 5 times the total weight of the raw materials of water to the extraction tank, soak for 30 minutes to make the raw materials fully absorb water and expand, and prepare for the subsequent extraction of active ingredients;

[0034] Step 4, first heating and reflux extraction: Adopt the heating and reflux extraction method, heat the extraction tank to boiling, maintain a slightly boiling state for extraction for 1 hour, and at the same time maintain the pressure in the extraction tank at 0.1 MPa to make the active ingredients in the raw materials fully dissolve into the water;

[0035] Step 5, first filtration: After the extraction is completed, filter the extract while it is hot to remove the medicinal residues, and obtain the first extract, initially separating the liquid containing the active ingredients;

[0036] Step 6, second water addition and extraction: Add 3 times the total weight of the raw materials of water to the medicinal residues, and perform heating and reflux extraction again. Similarly, heat to boiling and then maintain a slightly boiling state for extraction for 1 hour to further extract the residual active ingredients in the medicinal residues;

[0037] Step 7. Second filtration: After the second extraction is completed, filter the extract while it is still hot to obtain the second extract, thus completing the separation of the second active ingredient extract;

[0038] Step 8. Concentration treatment: Combine the first extract and the second extract, put them into a concentration device, and perform vacuum concentration at a temperature of 60 °C until the volume of the concentrated liquid is 1 / 4 of the total volume of the original extract, thereby increasing the concentration of the active ingredient;

[0039] Step 9. Adding preservatives and preparing the finished product: Cool the concentrated liquid to room temperature, add sodium benzoate accounting for 0.1% of the weight of the concentrated liquid, and stir evenly to obtain the pharmaceutical composition for treating diarrhea in calves.

[0040] During the heating reflux extraction process, maintain the pressure in the extraction tank at 0.1 - 0.3 MPa to promote the dissolution of the active ingredients in the raw materials.

[0041] The pharmaceutical composition can be made into an oral liquid dosage form. The oral liquid dosage form of the pharmaceutical composition also includes a flavoring agent, and the flavoring agent is sucrose or honey, and the addition amount is 5% - 10% of the weight of the concentrated liquid.

[0042] The pharmaceutical composition can be made into a granule dosage form. When preparing the granules, add a filler and a binder to the concentrated liquid. The filler is starch or dextrin, and the addition amount is 10% - 20% of the weight of the concentrated liquid. The binder is polyvinylpyrrolidone or hydroxypropyl methylcellulose, and the addition amount is 1% - 3% of the weight of the concentrated liquid.

[0043] Application of the pharmaceutical composition for treating diarrhea in calves in the preparation of a feed additive for preventing diarrhea in calves: Add the pharmaceutical composition to the feed of calves at a ratio of 0.5% - 2% to enhance the spleen and stomach function of calves and improve the body's resistance to diarrhea.

[0044] The third implementation method: A preparation method of a pharmaceutical composition for treating diarrhea in calves, including the following steps:

[0045] Step 1. Weighing raw materials: Weigh 15 parts of Coptis chinensis, 15 parts of Phellodendron amurense, 15 parts of Cortex Fraxini, 15 parts of Pulsatilla chinensis, 15 parts of Portulaca oleracea, 20 parts of Chinese yam, 20 parts of Poria cocos, 15 parts of Atractylodes macrocephala, and 10 parts of Glycyrrhiza uralensis;

[0046] Step 2. Pretreatment of raw materials: Wash the weighed raw materials respectively, remove impurities, then cut them into small pieces or sections, and put them into the extraction tank;

[0047] Step 3. Soaking in water: Add water 6 times the total weight of the raw materials to the extraction tank, soak for 45 minutes to make the raw materials fully absorb water and expand, and prepare for the subsequent extraction of active ingredients;

[0048] Step 4: First heat reflux extraction: Using the heat reflux extraction method, heat the extraction tank to boiling, maintain a slightly boiling state for extraction for 1.5 hours, and at the same time maintain the pressure in the extraction tank at 0.2 MPa to fully dissolve the active ingredients in the raw materials into water;

[0049] Step 5: First filtration: After the extraction is completed, filter the extract while it is hot to remove the medicinal residues, obtaining the first extract and initially separating the liquid containing the active ingredients;

[0050] Step 6: Second water addition and extraction: Add water 4 times the total weight of the raw materials to the medicinal residues, and conduct heat reflux extraction again. Similarly, heat to boiling and then maintain a slightly boiling state for extraction for 1.5 hours to further extract the remaining active ingredients in the medicinal residues;

[0051] Step 7: Second filtration: After the second extraction is completed, filter the extract while it is hot to obtain the second extract, completing the separation of the second extract of the active ingredients;

[0052] Step 8: Concentration treatment: Combine the first extract and the second extract, put them into a concentration device, and conduct vacuum concentration at a temperature of 70 °C until the volume of the concentrated solution is 1 / 4 of the total volume of the original extract, increasing the concentration of the active ingredients;

[0053] Step 9: Adding preservatives and preparing the finished product: Cool the concentrated solution to room temperature, add sodium benzoate at 0.15% of the weight of the concentrated solution, and stir evenly to obtain the pharmaceutical composition for treating calf yak diarrhea.

[0054] During the heat reflux extraction process, maintain the pressure in the extraction tank at 0.1 - 0.3 MPa to promote the dissolution of the active ingredients in the raw materials.

[0055] The pharmaceutical composition can be made into an oral liquid dosage form. The pharmaceutical composition in the oral liquid dosage form also includes a flavoring agent, and the flavoring agent is sucrose or honey, and the addition amount is 5% - 10% of the weight of the concentrated solution.

[0056] The pharmaceutical composition can be made into a granule dosage form. When preparing the granules, add a filler and a binder to the concentrated solution. The filler is starch or dextrin, and the addition amount is 10% - 20% of the weight of the concentrated solution. The binder is polyvinylpyrrolidone or hydroxypropyl methylcellulose, and the addition amount is 1% - 3% of the weight of the concentrated solution.

[0057] Application of the pharmaceutical composition for treating calf yak diarrhea in the preparation of a feed additive for preventing calf yak diarrhea: Add the pharmaceutical composition to the feed of calf yaks at a ratio of 0.5% - 2% to enhance the spleen and stomach functions of calf yaks and improve the body's resistance to diarrhea.

[0058] The fourth implementation method: A preparation method of a pharmaceutical composition for treating diarrhea in calves, comprising the following steps:

[0059] Step 1, raw material weighing: Weigh 20 parts of Coptis chinensis, 20 parts of Phellodendron amurense, 20 parts of Cortex Fraxini, 20 parts of Pulsatilla chinensis, 20 parts of Portulaca oleracea, 30 parts of Chinese yam, 30 parts of Poria cocos, 20 parts of Atractylodes macrocephala, and 15 parts of Glycyrrhiza uralensis;

[0060] Step 2, raw material pretreatment: Wash the weighed raw materials respectively, remove impurities, then cut them into small pieces or sections, and put them into the extraction tank;

[0061] Step 3, water soaking: Add water 8 times the total weight of the raw materials to the extraction tank, soak for 60 minutes to make the raw materials fully absorb water and expand, and prepare for the subsequent extraction of active ingredients;

[0062] Step 4, first heating reflux extraction: Adopt the heating reflux extraction method, heat the extraction tank to boiling, keep it in a slightly boiling state for extraction for 2 hours, and at the same time keep the pressure in the extraction tank at 0.3 MPa to make the active ingredients in the raw materials fully dissolve into the water;

[0063] Step 5, first filtration: After the extraction is completed, filter the extract while it is hot to remove the medicinal residues, and obtain the first extract, initially separating the liquid containing the active ingredients;

[0064] Step 6, second water addition and extraction: Add water 5 times the total weight of the raw materials to the medicinal residues, and perform heating reflux extraction again. Similarly, heat to boiling and then keep it in a slightly boiling state for extraction for 2 hours to further extract the residual active ingredients in the medicinal residues;

[0065] Step 7, second filtration: After the second extraction is completed, filter the extract while it is hot to obtain the second extract, and complete the separation of the second extract of active ingredients;

[0066] Step 8, concentration treatment: Combine the first extract and the second extract, put them into the concentration device, and perform reduced pressure concentration at a temperature of 80 °C until the volume of the concentrated solution is 1 / 5 of the total volume of the original extract, to increase the concentration of the active ingredients;

[0067] Step 9, adding preservatives and finished product preparation: Cool the concentrated solution to room temperature, add 0.2% of sodium benzoate based on the weight of the concentrated solution, and stir evenly to obtain the pharmaceutical composition for treating diarrhea in calves.

[0068] During the heating reflux extraction process, keep the pressure in the extraction tank at 0.1 - 0.3 MPa to promote the dissolution of the active ingredients in the raw materials.

[0069] The pharmaceutical composition can be made into an oral liquid dosage form. The pharmaceutical composition in the oral liquid dosage form further includes a flavoring agent, and the flavoring agent is sucrose or honey, and the added amount is 5% - 10% of the weight of the concentrated solution.

[0070] The pharmaceutical composition can be made into a granule dosage form. When preparing the granules, a filler and a binder are added to the concentrated solution. The filler is starch or dextrin, and the added amount is 10% - 20% of the weight of the concentrated solution. The binder is polyvinylpyrrolidone or hydroxypropyl methylcellulose, and the added amount is 1% - 3% of the weight of the concentrated solution.

[0071] Application of the pharmaceutical composition for treating diarrhea in calves in the preparation of a feed additive for preventing diarrhea in calves: The pharmaceutical composition is added to the feed of calves at a ratio of 0.5% - 2% to enhance the spleen and stomach functions of calves and improve the body's resistance to diarrhea.

[0072] The fifth embodiment: The first embodiment:

[0073] Step 1. Raw material weighing: 15 parts of Coptis chinensis, 15 parts of Phellodendron amurense, 15 parts of Cortex Fraxini, 12 parts of Pulsatilla chinensis, 20 parts of Portulaca oleracea, 30 parts of Dioscorea opposita, 30 parts of Poria cocos, 20 parts of Atractylodes macrocephala, 6 parts of Glycyrrhiza uralensis.

[0074] Step 2. Raw material pretreatment: Wash the weighed raw materials respectively, remove impurities, then cut them into small pieces or sections, and put them into the extraction tank;

[0075] Step 3. Water soaking: Add water 8 times the total weight of the raw materials to the extraction tank, soak for 60 minutes to make the raw materials fully absorb water and expand, and prepare for the subsequent extraction of active ingredients;

[0076] Step 4. First heating and reflux extraction: Adopt the heating and reflux extraction method, heat the extraction tank to boiling, keep it in a slightly boiling state for extraction for 2 hours, and at the same time keep the pressure in the extraction tank at 0.3 MPa to make the active ingredients in the raw materials fully dissolve into the water;

[0077] Step 5. First filtration: After the extraction is completed, filter the extraction liquid while it is hot to remove the medicinal residues and obtain the first extraction liquid, and initially separate the liquid containing the active ingredients;

[0078] Step 6. Second water addition and extraction: Add water 5 times the total weight of the raw materials to the medicinal residues, and carry out heating and reflux extraction again. Similarly, heat to boiling and then keep it in a slightly boiling state for extraction for 2 hours to further extract the residual active ingredients in the medicinal residues;

[0079] Step 7. Second filtration: After the second extraction is completed, filter the extraction liquid while it is hot to obtain the second extraction liquid and complete the separation of the second extraction liquid of the active ingredients;

[0080] Step 8. Concentration treatment: Combine the first extract and the second extract, put them into a concentration device, and perform reduced-pressure concentration at a temperature of 80°C until the volume of the concentrated solution is 1 / 5 of the total volume of the original extract, so as to increase the concentration of the active ingredients.

[0081] Step 9. Adding preservatives and preparing the finished product: Cool the concentrated solution to room temperature, add sodium benzoate accounting for 0.2% of the weight of the concentrated solution, and stir evenly to obtain a pharmaceutical composition for treating diarrhea in calves.

[0082] During the heating reflux extraction process, maintain the pressure in the extraction tank at 0.1 - 0.3 MPa to promote the dissolution of the active ingredients in the raw materials.

[0083] The pharmaceutical composition can be made into an oral liquid dosage form. The pharmaceutical composition in the oral liquid dosage form also includes a flavoring agent, and the flavoring agent is sucrose or honey, and the added amount is 5% - 10% of the weight of the concentrated solution.

[0084] The pharmaceutical composition can be made into a granule dosage form. When preparing the granules, add a filler and a binder to the concentrated solution. The filler is starch or dextrin, and the added amount is 10% - 20% of the weight of the concentrated solution. The binder is polyvinylpyrrolidone or hydroxypropyl methylcellulose, and the added amount is 1% - 3% of the weight of the concentrated solution.

[0085] Application of the pharmaceutical composition for treating diarrhea in calves in the preparation of a feed additive for preventing diarrhea in calves: Add the pharmaceutical composition to the feed of calves at a ratio of 0.5% - 2% to enhance the spleen and stomach function of calves and improve the body's resistance to diarrhea.

[0086] Through clinical trials on multiple calves suffering from diarrhea, using the pharmaceutical composition prepared in Examples 2 - 4 of the present invention, at a dose of 0.5 - 1 ml per kilogram of body weight, administered orally 2 - 3 times a day, and used continuously for 3 - 5 days, the results showed that the diarrhea symptoms of the calves were significantly improved, the feces gradually returned to normal, the mental state and appetite were significantly improved, the cure rate reached over 85%, and there were no obvious adverse reactions.

[0087] It can be seen that the present application uses a variety of natural Chinese medicinal materials as raw materials, which are safe and have no side effects, and will not have an adverse impact on the meat quality and dairy product quality of calves, meeting the requirements of green farming and food safety. The raw materials in the pharmaceutical composition are mutually compatible and have a synergistic effect. They can not only effectively inhibit and kill pathogens such as bacteria, viruses, and parasites causing diarrhea, but also regulate the spleen and stomach function of calves, enhance the body's resistance, fundamentally treat diarrhea in calves, and are not prone to recurrence.

[0088] Moreover, it is easy to operate. Through reasonable extraction and concentration processes, it can maximize the extraction of active ingredients from raw materials, improve the efficacy of the pharmaceutical composition. The generated pharmaceutical composition can be made into different dosage forms according to actual situations, such as oral liquids, granules, etc., which is convenient for administration and improves the medication compliance of calves.

[0089] Meanwhile, the content not described in detail in this specification belongs to the prior art well-known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used.

[0090] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device.

[0091] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A pharmaceutical composition for treating diarrhea in calves and yaks, characterized in that: The invention is prepared from the following raw materials in parts by weight: 10-20 parts of coptis root, 10-20 parts of phellodendron, 10-20 parts of qinpi, 10-20 parts of pulsatilla, 10-20 parts of purslane, 15-30 parts of yam, 15-30 parts of tuckahoe, 10-20 parts of atractylodes and 5-15 parts of liquorice.

2. A method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks, which is used to prepare the pharmaceutical composition according to claim 1, characterized in that: The following steps are involved: Step 1, weighing raw materials: weighing raw materials of coptis root, phellodendron, qinpi, pulsatilla, purslane, yam, tuckahoe, atractylodes and liquorice according to the above weight parts; Step 2: Raw material pretreatment: Wash the weighed raw materials separately, remove impurities, cut them into small pieces or segments, and put them into the extraction tank; Step 3: Soak in water: Add 5-8 times the total weight of the raw materials into the extraction tank and soak for 30-60 minutes to allow the raw materials to fully absorb water and swell, in preparation for the subsequent extraction of active ingredients; Step 4: First heating reflux extraction: Using the heating reflux extraction method, heat the extraction tank to boiling, maintain a slightly boiling state for 1-2 hours, so that the effective ingredients in the raw materials are fully dissolved into the water; Step 5, first filtration: After the extraction is completed, the extract is filtered while hot to remove the drug residue to obtain the first extract, and the liquid containing the active ingredient is preliminarily separated; Step 6, adding water and extracting for the second time: adding water 3 to 5 times the total weight of the raw materials to the residue, heating and refluxing extraction again, heating to boiling and maintaining a slightly boiling state for 1 to 2 hours, further extracting the effective components remaining in the residue; Step 7, second filtration: After the second extraction is completed, the extract is filtered while hot to obtain the second extract, completing the separation of the second effective component extract; Step 8, concentration treatment: combine the first extract and the second extract, put them into a concentration device, and concentrate them under reduced pressure at a temperature of 60-80°C until the volume of the concentrate is 1 / 4-1 / 5 of the total volume of the original extract, thereby increasing the concentration of the active ingredient; Step nine, adding preservatives and preparing finished products: cooling the concentrated solution to room temperature, adding a proper amount of preservatives, stirring evenly, and obtaining a pharmaceutical composition for treating diarrhea in calves and yaks.

3. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: The preservative is sodium benzoate, and the added amount is 0.1% to 0.2% of the weight of the concentrated solution.

4. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: The weight ratio of the coptis chinensis, phellodendron, qinpi, pulsatilla, purslane, yam, poria, atractylodes macrocephala and licorice is: 12-18 parts of coptis chinensis, 12-18 parts of phellodendron, 12-18 parts of qinpi, 12-18 parts of pulsatilla, 12-18 parts of purslane, 18-25 parts of yam, 18-25 parts of poria, 12-18 parts of atractylodes macrocephala and 8-12 parts of licorice.

5. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: During the heating reflux extraction process, the pressure in the extraction tank is maintained at 0.1-0.3 MPa to promote the dissolution of the effective ingredients in the raw materials.

6. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: The pharmaceutical composition can be prepared into an oral liquid dosage form. The pharmaceutical composition in the oral liquid dosage form also includes a flavoring agent, which is sucrose or honey, and the added amount is 5% to 10% of the weight of the concentrated liquid.

7. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: The pharmaceutical composition can be prepared into a granular dosage form. When preparing the granules, a filler and a binder are added to the concentrated solution. The filler is starch or dextrin, and the added amount is 10% to 20% of the weight of the concentrated solution. The binder is polyvinyl pyrrolidone or hydroxypropyl methylcellulose, and the added amount is 1% to 3% of the weight of the concentrated solution.

8. The method for preparing a pharmaceutical composition for treating diarrhea in calves and yaks according to claim 2, characterized in that: The application of the pharmaceutical composition for treating calf yak diarrhea in the preparation of a feed additive for preventing calf yak diarrhea: the pharmaceutical composition is added to calf yak feed at a ratio of 0.5% to 2% to enhance the spleen and stomach function of the calf yak and improve the body's resistance to diarrhea.

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