Reagent transfer structure, molecular diagnosis reagent card box and molecular diagnosis equipment

Abstract

The embodiment of the invention provides a reagent transfer structure, a molecular diagnosis reagent card box and molecular diagnosis equipment, and relates to the field of molecular detection. The reagent transfer structure comprises a carrier and a liquid transfer piece, the carrier piece comprises a first cavity and a second cavity, the first cavity is used for containing a liquid reagent, and the first cavity and the second cavity are communicated through a first flow path; the liquid transfer part is arranged on the first flow path and used for controlling the first flow path to be connected and disconnected at the first position, and the liquid transfer part communicates with the second cavity at the second position and used for controlling the pressure intensity in the second cavity; the first position is different from the second position, and when the liquid transfer piece is located at the first position, a pressure difference exists between the second cavity and the first cavity. According to the reagent transfer structure, reagent transfer structure components can be reduced, the manufacturing cost is reduced, and the liquid transfer operation is simplified.

Description

Technical Field

[0001] The present invention relates to the field of molecular detection, and more particularly, to a reagent transfer structure, a molecular diagnostic reagent cartridge, and a molecular diagnostic device. Background Art

[0002] Biochemical detection requires many operation steps and is generally completed by professionals. Taking molecular detection based on polymerase chain nucleic acid amplification as an example, the whole process includes steps such as nucleic acid extraction, amplification, and detection. In these processes, various solutions and reagents are involved, and there are many liquid transfer steps, which are relatively complex. Currently, most reagent transfer structures are provided with a negative pressure mechanism and a liquid on-off valve to perform the liquid transfer step.

[0003] However, most of the current reagent transfer structures have a complex structure, many structural components, a high manufacturing cost, and a complex liquid transfer operation. Summary of the Invention

[0004] The present invention provides a reagent transfer structure, a molecular diagnostic reagent cartridge, and a molecular diagnostic device, which can reduce the components of the reagent transfer structure, reduce the manufacturing cost, and simplify the liquid transfer operation.

[0005] Embodiments of the present invention may be implemented as follows:

[0006] An embodiment of the present invention provides a reagent transfer structure, which includes:

[0007] A carrier member, the carrier member includes a first chamber and a second chamber, the first chamber is used for accommodating a liquid reagent, and the first chamber and the second chamber are communicated through a first flow path;

[0008] A liquid transfer member, the liquid transfer member is disposed on the first flow path and is used for controlling the on-off of the first flow path at a first position, and the liquid transfer member is communicated with the second chamber at a second position for controlling the pressure in the second chamber;

[0009] The first position is different from the second position. When the liquid transfer member is at the first position, there is a pressure difference between the second chamber and the first chamber.

[0010] In an optional embodiment, the second chamber is formed by a tube body that is detachably connected to the carrier member.

[0011] In an optional embodiment, the liquid transfer member includes a transfer chamber and a moving member, the transfer chamber is disposed on the first flow path of the carrier member, the transfer chamber is communicated with the second chamber, and the moving member is movably and sealably relative to the transfer chamber;

[0012] The moving part has a liquid channel. When the moving part moves to the first position, the first flow path is communicated through the liquid channel.

[0013] In an alternative embodiment, the first flow path has a first port at the first position of the transfer chamber, and the outer periphery of the liquid channel of the moving part has a second port. The first port and the second port are connected at the first position to communicate the first flow path.

[0014] In an alternative embodiment, the moving part and the transfer chamber form a variable sealed chamber, and the variable sealed chamber communicates with the second chamber at the second position.

[0015] In an alternative embodiment, the second position is located at the bottom of the transfer chamber.

[0016] In an alternative embodiment, the moving part moves in the first direction in the transfer chamber to communicate or block the first flow path.

[0017] In an alternative embodiment, the transfer chamber is provided with a guiding structure along the first direction to enable the moving part to move along the first direction.

[0018] An embodiment of the present invention further provides a molecular diagnostic reagent cartridge, which includes a cartridge body including a plurality of accommodating chambers and the reagent transfer structure in any of the above embodiments, and the first chamber is an elution chamber.

[0019] An embodiment of the present invention further provides a molecular diagnostic device, which includes a detection platform, a moving mechanism, and the molecular diagnostic reagent cartridge in the above embodiment. The detection platform is used to place the molecular diagnostic reagent cartridge;

[0020] The moving mechanism is used to drive the liquid transfer part to move up and down, so that the liquid transfer part controls the on-off of the first flow path at the first position.

[0021] The beneficial effects of the reagent transfer structure, the molecular diagnostic reagent cartridge, and the molecular diagnostic device in the embodiments of the present invention include:

[0022] By arranging the liquid transfer member on the first flow path, the liquid transfer member can connect or block the first chamber and the second chamber, and the liquid transfer member is connected to the second chamber at the second position, and the movement of the liquid transfer member can make a pressure difference between the second chamber and the first chamber. When the liquid transfer member is connected to the first flow path, the first chamber and the second chamber are connected, and a negative pressure is formed in the second chamber, and the liquid is automatically transferred from the first chamber to the second chamber through the connecting flow path; when the liquid transfer member blocks the first flow path, the first chamber and the second chamber are not connected, and the liquid is not transferred and is still stored in the first chamber. The liquid transfer member can connect or block the first chamber and the second chamber, and can control the pressure difference between the first chamber and the second chamber to achieve automatic transfer. Therefore, liquid transfer can be achieved by only manipulating the movement of the liquid transfer member, which is simple to operate and has fewer structural components. While improving the transfer efficiency, it saves manufacturing costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 A schematic diagram of a reagent transfer structure provided in an embodiment of the present invention;

[0025] Figure 2 A schematic diagram of a moving part provided in an embodiment of the present invention;

[0026] Figure 3 A schematic diagram of a moving part with a sealing rubber sleeve provided in an embodiment of the present invention;

[0027] Figure 4 It is a schematic diagram of a first viewing angle of a molecular diagnostic reagent cartridge in a blocked state provided in an embodiment of the present invention;

[0028] Figure 5 It is a schematic diagram of a second viewing angle of a molecular diagnostic reagent cartridge in a blocked state provided in an embodiment of the present invention;

[0029] Figure 6 A schematic diagram of an AA section provided in an embodiment of the present invention;

[0030] Figure 7 It is a schematic diagram of a first viewing angle when the molecular diagnostic reagent cartridge provided in an embodiment of the present invention is in a connected state;

[0031] Figure 8Schematic diagram of the second perspective when the molecular diagnostic reagent cartridge is in a connected state provided in the embodiment of the present invention;

[0032] Figure 9 Schematic diagram of the B-B section provided in the embodiment of the present invention.

[0033] Icons: 10 - Molecular diagnostic reagent cartridge; 1 - Cartridge body; 1000 - Reagent transfer structure; 100 - Carrier member; 110 - First chamber; 120 - Second chamber; 122 - Solution chamber; 130 - Connecting flow path; 131 - First flow path; 1311 - First port; 132 - Second flow path; 1321 - Horizontal channel; 1322 - Vertical channel; 200 - Liquid transfer member; 210 - Transfer chamber; 211 - Third flow path; 220 - Moving member; 221 - Liquid channel; 222 - Second port; 223 - Sealing rubber sleeve; 224 - First rod portion; 225 - Second rod portion; 300 - Variable sealed chamber; 310 - Sealed cavity; 400 - Plugging member; 500 - Accommodating chamber. Detailed implementation manners

[0034] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Usually, the components of the embodiments of the present invention described and illustrated in the accompanying drawings here can be arranged and designed in various different configurations.

[0035] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed present invention, but merely represents the selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0036] It should be noted that: similar reference numerals and letters denote similar items in the following drawings. Therefore, once an item is defined in one drawing, it does not need to be further defined and explained in subsequent drawings.

[0037] In the description of the present invention, it should be noted that if terms such as "upper", "lower", "inner", "outer", etc. are used to indicate the orientation or positional relationship, it is based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the present invention is usually placed when in use. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation to the present invention.

[0038] In addition, terms such as "first" and "second" are only used for distinguishing descriptions and should not be construed as indicating or implying relative importance.

[0039] It should be noted that, without conflict, the features in the embodiments of the present invention can be combined with each other.

[0040] Biochemical detection requires many operation steps and can generally be completed only by professionals. Taking molecular detection based on polymerase chain nucleic acid amplification as an example, the whole process includes steps such as nucleic acid extraction, amplification, and detection. In these processes, various solutions and reagents are involved, and there are many pipetting steps, which are relatively complex. At present, most reagent transfer structures are provided with a negative pressure mechanism and a liquid on-off valve to perform the liquid transfer step. However, most of the current reagent transfer structures are complex in structure, have many structural components, high manufacturing costs, and complex liquid transfer operations.

[0041] Based on this, please refer to Figure 1 , the reagent transfer structure 1000 provided in the embodiments of the present invention can effectively improve the above-mentioned technical problems. The reagent transfer structure 1000 reduces the components of the reagent transfer structure 1000, reduces the manufacturing cost, and simplifies the liquid transfer operation. The reagent transfer structure 1000 is applied to the molecular diagnostic reagent cartridge 10, and the molecular diagnostic reagent cartridge 10 is applied to a molecular detection device. Both the molecular diagnostic reagent cartridge 10 and the molecular detection device have the characteristics of the above-mentioned reagent transfer structure 1000, and can also achieve liquid transfer through simple operations, simplify the structure, and reduce the manufacturing cost.

[0042] Figure 1 is a schematic diagram of the reagent transfer structure 1000 provided in the embodiments of the present invention. As Figure 1 shown, the reagent transfer structure 1000 provided in the embodiments of the present invention includes a carrier and a liquid transfer member 200. The carrier member 100 includes a first chamber 110 and a second chamber 120. The first chamber 110 is used to accommodate a liquid reagent, and the first chamber 110 and the second chamber 120 are connected through a first flow path 131. The liquid transfer member 200 is disposed on the first flow path 131 and is used to control the on-off of the first flow path 131 at a first position. The liquid transfer member 200 is connected to the second chamber 120 at a second position and is used to control the pressure in the second chamber 120. The first position is different from the second position. When the liquid transfer member 200 is at the first position, there is a pressure difference between the second chamber 120 and the first chamber 110.

[0043] By arranging the liquid transfer member 200 on the first flow path 131, the liquid transfer member 200 can connect or block the first chamber 110 and the second chamber 120, and the liquid transfer member 200 is connected to the second chamber 120 at the second position, and the movement of the liquid transfer member 200 can make a pressure difference between the second chamber 120 and the first chamber 110. When the liquid transfer member 200 is connected to the first flow path, the first chamber 110 and the second chamber 120 are connected, and a negative pressure is formed in the second chamber 120, and the liquid is automatically transferred from the first chamber 110 to the second chamber 120 through the connecting flow path 130; when the liquid transfer member 200 blocks the first flow path, the first chamber 110 and the second chamber 120 are not connected, and the liquid is not transferred and is still stored in the first chamber 110. The liquid transfer member 200 can connect or block the first chamber 110 and the second chamber 120, and can control the pressure difference between the first chamber 110 and the second chamber 120 to achieve automatic liquid transfer. Therefore, liquid transfer can be achieved by only manipulating the movement of the liquid transfer member 200. The operation is simple and has fewer structural components. While improving the transfer efficiency, the manufacturing cost is saved.

[0044] Please continue reading Figure 1 The liquid transfer member 200 in this embodiment includes a transfer chamber 210 and a moving member 220. The transfer chamber 210 is arranged on the first flow path 131 of the carrier member 100. The transfer chamber 210 is connected to the second chamber 120. The moving member 220 and the transfer chamber 210 can move relative to each other in a sealed manner. The moving member 220 has a liquid channel 221. When the moving member 220 moves to the first position, the first flow path 131 is connected through the liquid channel 221. Specifically, the first flow path 131 has a first port 1311 at the first position of the transfer chamber 210, and the outer periphery of the liquid channel 221 of the moving member 220 has a second port 222. The first port 1311 and the second port 222 are connected at the first position to make the first flow path 131 connected. When the moving member 220 moves to the first position of the transfer chamber 210, the second port 222 is aligned with the first port 1311 and connected, and the first flow path 131 is connected to the liquid channel 221, that is, the first chamber 110 is connected to the second chamber 120.

[0045] In this embodiment, the moving member 220 moves in the transfer chamber 210 along the first direction X to connect or block the first flow path 131. That is, when the moving member 220 moves relative to the transfer chamber 210 along the first direction X, at the first position, when the first port 1311 and the second port 222 are aligned and connected, the first flow path 131 is connected, and the liquid in the first chamber 110 can flow into the second chamber 120 through the first flow path 131 and the liquid channel 221. In order to facilitate the moving member 220 to move relative to the transfer chamber 210 along the first direction X, the transfer chamber 210 in this embodiment is provided with a guide structure along the first direction X to enable the moving member 220 to move along the first direction X.

[0046] In this embodiment, the moving part 220 and the transfer chamber 210 form a variable sealed chamber 300, and the variable sealed chamber 300 communicates with the second chamber 120 at the second position. During the movement of the moving part 220 relative to the transfer chamber 210, the volume of the variable sealed chamber 300 changes. Since the variable sealed chamber 300 communicates with the second chamber 120, the pressure in the variable sealed chamber 300 changes due to the volume change, and the pressure in the second chamber 120 also changes. Therefore, there is a pressure difference between the first chamber 110 and the second chamber 120, and the liquid can automatically flow from the first chamber 110 into the second chamber 120 due to the pressure difference. In this embodiment, the second position is located at the bottom of the transfer chamber 210. Of course, the second position can also be set on the side wall of the transfer chamber 210, as long as the second position is lower than the first position, and as long as it is ensured that when the first flow path 131 is connected, the pressure in the variable sealed chamber 300 is lower than the pressure in the first chamber 110. The specific setting position of the second position is not limited here.

[0047] Specifically, in this embodiment, the second chamber 120 includes a liquid storage chamber 122. The transfer chamber 210 and the first chamber 110 are connected through a first flow path 131. The moving part 220 is arranged in the transfer chamber 210, and a part of the moving part 220 and the transfer chamber 210 forms a variable sealed chamber 300. The liquid storage chamber 122 has a sealed cavity 310 for storing liquid reagents. The transfer chamber 210 is connected to the liquid storage chamber 122. A second flow path 132 is opened on the side wall of the first position of the transfer chamber 210 away from the first flow path 131. The communication flow path 130 includes the first flow path 131 and the second flow path 132. The first position of the transfer chamber 210 communicates with the sealed cavity 310 through the second flow path 132. A third flow path 211 is opened at the second position of the transfer chamber 210. The variable sealed chamber 300 communicates with the sealed cavity 310 through the third flow path 211. The moving part 220 is provided with a liquid channel 221. The moving part 220 is used to connect the first flow path 131 and the second flow path 132 through the liquid channel 221 during the movement relative to the transfer chamber 210, and to form a negative pressure in the variable sealed chamber 300 and the sealed cavity 310, so that the liquid in the first chamber 110 sequentially enters the sealed cavity 310 through the first flow path 131, the liquid channel 221 and the second flow path 132.

[0048] The moving part 220 moves relative to the transfer cavity 210, so that the liquid channel 221 of the moving part 220 connects the first flow path 131 and the second flow path 132, that is, the first chamber 110 communicates with the sealed cavity 310 of the liquid containing cavity 122. The liquid in the first chamber 110 can flow from the first chamber 110 through the first flow path 131, the liquid channel 221 and the second flow path 132 into the liquid containing cavity 122. Since the third flow path 211 is provided at the second position of the transfer cavity 210, the variable sealed cavity 300 communicates with the sealed cavity 310 through the third flow path 211, that is, the pressures in the variable sealed cavity 300 and the sealed cavity 310 are the same. When the moving part 220 moves relative to the transfer cavity 210, since the volume of the variable sealed cavity 300 changes, the pressures in the variable sealed cavity 300 and the sealed cavity 310 also change accordingly. When the volume of the variable sealed cavity 300 increases, the pressures in the variable sealed cavity 300 and the sealed cavity 310 decrease, that is, a negative pressure is generated in the variable sealed cavity 300 and the sealed cavity 310.

[0049] Please continue to refer to Figure 1 and in combination with Figure 6 and Figure 9 , Figure 6 is a schematic diagram of the A-A cross-section provided in the embodiment of the present invention; Figure 9 is a schematic diagram of the B-B cross-section provided in the embodiment of the present invention. In the initial state of this embodiment, that is, when the first chamber 110 and the liquid containing cavity 122 are not connected, the moving part 220 is in a state where the liquid channel 221 does not connect the first flow path 131 and the second flow path 132, and the position of the liquid channel 221 is lower than the positions of the first flow path 131 and the second flow path 132. Then when the liquid transfer part 200 moves relative to the first chamber 110 to connect the liquid channel 221 with the first flow path 131 and the second flow path 132, the volume of the variable sealed cavity 300 increases, and the pressures in the variable sealed cavity 300 and the connected sealed cavity 310 decrease, forming a negative pressure. At this time, the liquid in the first chamber 110 automatically flows from the first chamber 110 through the first flow path 131, the liquid channel 221 and the second flow path 132 into the sealed cavity 310 of the liquid containing cavity 122. That is, only by moving the moving part 220 to connect the first flow path 131 and the second flow path 132 can the liquid transfer operation be realized. While simplifying the liquid transfer structure, the liquid transfer operation is also simplified.

[0050] Please continue to refer to Figure 1, generally, other reagents are added to the general solution chamber 122 to react with the reagent to be detected. To facilitate the cleaning of the solution chamber 122 and achieve reuse, in this embodiment, the second chamber 120 is formed by a tube body detachably connected to the carrier member 100. Specifically, the second chamber in this embodiment is a solution chamber, and the transfer chamber 210 and the solution chamber 122 are detachably connected. Connection methods such as snap connection or screw connection can be used, which are not limited herein. To prevent liquid from leaking from the connection, a sealing gasket is provided at the connection between the transfer chamber 210 and the solution chamber 122. In addition, the transfer chamber 210 and the solution chamber 122 can also be fixedly connected or integrally formed, which are not limited herein.

[0051] Please continue to refer to Figure 1 , and in combination with Figure 6 and Figure 9 , to facilitate the injection molding of the first flow path 131 and the second flow path 132, forming holes are provided at the radial positions of partial flow segments of the first flow path 131 and the second flow path 132. The second flow path 132 in this embodiment includes a horizontal channel 1321 and a vertical channel 1322. The horizontal channel 1321 and the first flow path 131 are on the same horizontal plane, and the vertical channel 1322 communicates with the horizontal channel 1321 and the sealed cavity 310. The liquid transfer member 200 is used to connect or block the first flow path 131 and the horizontal channel 1321. To be able to block the formed flow channel to avoid liquid leakage and facilitate mold opening manufacturing, a blocking member 400 is provided at one end of the horizontal channel 1321 away from the liquid transfer member 200.

[0052] Figure 2 is a schematic diagram of the moving member 220 provided in the embodiment of the present invention; Figure 3 is a schematic diagram of the moving member 220 with a sealing rubber sleeve 223 provided in the embodiment of the present invention. To prevent the liquid in the first flow path 131 from leaking into the second chamber 120, please refer to Figure 2 and Figure 3In this embodiment, the moving part 220 includes a first rod 224 and a second rod 225. The first rod 224 is connected to the second rod 225. The second rod 225 is provided with a liquid channel 221, and the liquid channel 221 is used to connect the first flow path 131 and the second flow path 132. The second rod 225 is located in the transfer chamber 210, and the first rod 224 is used to drive the second rod 225 to move relative to the transfer chamber 210. The second rod 225 is sleeved with a sealing rubber sleeve 223, and the sealing rubber sleeve 223 is provided with a through hole at a position corresponding to the liquid channel 221. By providing the sealing rubber sleeve 223, it can be achieved that when the first flow path 131, the liquid channel 221 and the second flow path 132 are connected, the liquid does not leak from the connection between the liquid channel 221 and the first flow path 131 and the second flow path 132. In addition, the sealing rubber sleeve 223 or other sealing structures such as sealing gaskets and sealing rings can also be installed only on both sides of the liquid channel 221 to prevent liquid leakage. In order to prevent liquid leakage when the first flow path 131 and the second flow path 132 are not connected, a sealing structure may be provided around the first flow path 131. In order to save materials and reduce costs, the first rod portion 224 includes two cross-intersecting plate structures.

[0053] Figure 4 It is a schematic diagram of a first viewing angle of the molecular diagnostic reagent cartridge 10 provided in an embodiment of the present invention in a blocked state; Figure 5 for Figure 4 Schematic diagram from another perspective; Figure 7 It is a schematic diagram of a first viewing angle of a molecular diagnostic reagent cartridge 10 provided in an embodiment of the present invention when in a connected state; Figure 8 for Figure 7 Another view of the diagram. Figures 4 - 9 , and combined with Figure 1 The molecular diagnostic reagent cartridge 10 provided in this embodiment includes a plurality of accommodating chambers 500 and the reagent transfer structure 1000 in the above embodiment, and the first chamber 110 is an elution chamber. The reagent transfer structure 1000 and the plurality of accommodating chambers 500 are sequentially connected and integrated into one, and the first chamber 110 is arranged close to the plurality of accommodating chambers 500.

[0054] Taking molecular detection based on polymerase chain nucleic acid amplification as an example, the multiple accommodation cavities 500 of the molecular diagnostic reagent cartridge 10 in this embodiment are a first cleaning cavity, a second cleaning cavity, a third cleaning cavity, a magnetic bead cavity, a sample cavity, and a magnetic rod sleeve cavity. The first cleaning cavity, the second cleaning cavity, the third cleaning cavity, the magnetic bead cavity, the sample cavity, and the magnetic rod sleeve cavity are sequentially arranged from one end of the cartridge body 1 close to the first chamber 110 to the end of the cartridge body 1 far from the first chamber 110. The reagent to be detected is sequentially passed through the magnetic rod sleeve cavity, the sample cavity, the magnetic bead cavity, the third cleaning cavity, the second cleaning cavity, and the first cleaning cavity, and then transferred to the first chamber 110. Then, through a pipetting operation, the liquid in the first chamber 110 is transferred to the solution cavity 122 for reaction detection. The pipetting operation is the same as that of the above-mentioned reagent transfer structure 1000 and will not be elaborated here. To make the detection result more accurate, one or more cleaning cavities can be added between the first cleaning cavity and the second cleaning cavity. The magnetic rod sleeve compartment is used to carry the magnetic rod sleeve assembly, the sample compartment is used to hold the sample solvent, the magnetic bead compartment is used to hold the magnetic bead preservation solution, and the magnetic bead preservation solution contains magnetic beads. The cleaning compartment is used to hold the washing liquid, and both the first elution liquid compartment and the second elution compartment are used to hold the elution liquid. In addition, different accommodation cavities 500 can also be set according to the specific conditions of other reagent detections, which are not limited here.

[0055] The molecular detection device provided in this embodiment includes a detection platform, a motion mechanism, and the molecular diagnostic reagent cartridge 10 in the above embodiment. The molecular diagnostic reagent cartridge 10 is connected to the detection component. The detection platform is used to place the molecular diagnostic reagent cartridge 10; the motion mechanism is used to drive the liquid transfer member 200 to move up and down, so that the liquid transfer member 200 controls the on-off of the first flow path 131 at the first position. To realize the up and down movement of the liquid moving member relative to the second chamber 120 to achieve pipetting, in this embodiment, the motion mechanism is provided with a groove for clamping the liquid transfer member 200. By moving the motion mechanism up and down, the liquid transfer member 200 moves relative to the second chamber 120. Of course, the motion mechanism can also be connected to a grasping mechanism. By grasping the liquid transfer member 200 with the grasping mechanism and driving the grasping mechanism to move by the motion mechanism, the liquid transfer member 200 can also move relative to the second chamber 120. As long as the liquid transfer member 200 can be driven to move relative to the second chamber 120, the specific motion structure and method are not limited here.

[0056] According to a reagent transfer structure 1000 provided in this embodiment, its working principle is as follows:

[0057] In the initial state, that is, when the first chamber 110 and the liquid containing chamber 122, that is, the second chamber are not connected, the liquid transfer member 200 is in a state where the liquid channel 221 is not connected to the first flow path 131 and the second flow path 132. When the liquid transfer member 200 moves relative to the first chamber 110, so that the liquid channel 221 connects the first flow path 131 and the second flow path 132, the volume of the variable sealed chamber 300 increases, and the pressure in the variable sealed chamber 300 and the sealed containing chamber 310 connected thereto decreases, forming a negative pressure. At this time, the liquid in the first chamber 110 automatically flows from the first chamber 110 through the first flow path 131, the liquid channel 221 and the second flow path 132 into the sealed containing chamber 310 of the liquid containing chamber 122. That is, the liquid transfer operation can be achieved by only moving the liquid transfer member 200 to connect the first flow path 131 and the second flow path 132.

[0058] In summary, the reagent transfer structure 1000 includes a carrier and a liquid transfer member 200. The carrier member 100 includes a first chamber 110 and a second chamber 120. The first chamber 110 is used to contain a liquid reagent. The first chamber 110 and the second chamber 120 are connected through a first flow path 131. The liquid transfer member 200 is disposed on the first flow path 131 and is used to control the on-off of the first flow path 131 at a first position. The liquid transfer member 200 is connected to the second chamber 120 at a second position and is used to control the pressure in the second chamber 120. The first position is different from the second position. When the liquid transfer member 200 is at the first position, there is a pressure difference between the second chamber 120 and the first chamber 110.

[0059] By arranging the liquid transfer member 200 on the first flow path 131, the liquid transfer member 200 can connect or block the first chamber 110 and the second chamber 120, and the liquid transfer member 200 is connected to the second chamber 120 at the second position, and the movement of the liquid transfer member 200 can make a pressure difference between the second chamber 120 and the first chamber 110. When the liquid transfer member 200 is connected to the first flow path, the first chamber 110 and the second chamber 120 are connected, and a negative pressure is formed in the second chamber 120, and the liquid is automatically transferred from the first chamber 110 to the second chamber 120 through the connecting flow path 130; when the liquid transfer member 200 blocks the first flow path, the first chamber 110 and the second chamber 120 are not connected, and the liquid is not transferred and is still stored in the first chamber 110. The liquid transfer member 200 can connect or block the first chamber 110 and the second chamber 120, and can control the pressure difference between the first chamber 110 and the second chamber 120 to achieve automatic liquid transfer. Therefore, liquid transfer can be achieved by only manipulating the movement of the liquid transfer member 200. The operation is simple and has fewer structural components. While improving the transfer efficiency, the manufacturing cost is saved.

[0060] As described above, it is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention should be covered within the protection scope of the present invention.

Claims

1. A reagent transfer structure, characterized in that, Comprising: A carrier member (100), the carrier member (100) comprising a first chamber (110) and a second chamber (120), the first chamber (110) being adapted to contain a liquid reagent, and the first chamber (110) and the second chamber (120) being in communication via a first flow path (131); A liquid transfer member (200), the liquid transfer member (200) being disposed on the first flow path (131) for controlling the opening and closing of the first flow path (131) at a first position, and the liquid transfer member (200) being in communication with the second chamber (120) at a second position for controlling the pressure within the second chamber (120); The first position is different from the second position. When the liquid transfer member (200) is at the first position, there is a pressure difference between the second chamber (120) and the first chamber (110).

2. The reagent transfer structure according to claim 1, characterized in that, The second chamber (120) is formed by a tube body that is detachably connected to the carrier member (100).

3. The reagent transfer structure according to claim 1, characterized in that, The liquid transfer member (200) comprises a transfer chamber (210) and a moving member (220), the transfer chamber (210) being disposed on the first flow path (131) of the carrier member (100), the transfer chamber (210) being in communication with the second chamber (120), and the moving member (220) being movably and sealingly relative to the transfer chamber (210); The moving member (220) has a liquid channel (221). When the moving member (220) moves to the first position, the first flow path (131) is in communication via the liquid channel (221).

4. The reagent transfer structure according to claim 3, characterized in that, The first flow path (131) has a first port (1311) at the first position of the transfer chamber (210), and a second port (222) is provided on the outer periphery of the liquid channel (221) of the moving member (220). The first port (1311) and the second port (222) are connected at the first position to enable the first flow path (131) to be in communication.

5. The reagent transfer structure according to claim 3, characterized in that, The moving member (220) and the transfer chamber (210) form a variable sealed chamber (300), and the variable sealed chamber (300) is in communication with the second chamber (120) at the second position.

6. The reagent transfer structure according to claim 5, characterized in that, The second position is located at the bottom of the transfer chamber (210).

7. The reagent transfer structure according to claim 3, characterized in that, The moving member (220) moves in a first direction within the transfer chamber (210) to enable or block the first flow path (131).

8. The reagent transfer structure according to claim 7, characterized in that, The transfer chamber (210) is provided with a guiding structure along the first direction to enable the moving member (220) to move along the first direction.

9. A molecular diagnostic reagent cartridge, characterized in that, Comprising a cartridge body (1), the cartridge body (1) comprising a plurality of receiving chambers (500) and a reagent transfer structure (1000) according to any one of claims 1-8, and the first chamber (110) being an elution chamber.

10. A molecular diagnostic device, characterized in that, Comprising a detection platform, a motion mechanism, and a molecular diagnostic reagent cartridge (10) according to claim 9, the detection platform being adapted to place the molecular diagnostic reagent cartridge (10); The motion mechanism is used to drive the liquid transfer member (200) to move up and down, so that the liquid transfer member (200) controls the on-off of the first flow path (131) at the first position.

Images