Highly-bionic one-way diversion artificial lymphatic vessel and high-precision controllable preparation method thereof

Abstract

The invention discloses a highly bionic one-way diversion artificial lymphatic vessel and a high-precision controllable preparation method thereof.The preparation method comprises the steps that size parameters of a one-way diversion unit of a target artificial lymphatic vessel are determined, an artificial lymphatic vessel mold is prepared, a degradable biological material is directly dissolved with a solvent, and a solution with certain viscosity is obtained; coating a smooth plane with the film, quickly rolling the working surface of the mold towards the plane when the solvent is volatilized and the material is nearly cured, continuously volatilizing the solvent and completely curing the material on the surface of the mold to form a film, cutting off the part which is not printed with the one-way flow guide structure by using a cutter, and curling the film into a pipe along the direction vertical to the one-way flow guide unit. The highly-bionic one-way diversion artificial lymphatic vessel with the one-way diversion trench is prepared through a high-precision controllable method, one-way flow of liquid can be controlled, lymphatic vessel regeneration and directional diversion of lymph liquid are effectively induced, and the used materials are high in biological safety and free of untoward effects such as sensitization and teratogenesis.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical materials, and particularly relates to a highly biomimetic unidirectional flow artificial lymphatic vessel and a high-precision controllable preparation method thereof. Background Art

[0002] The lymphatic circulatory system is a key component of the body's defense mechanism, closely connected to the blood system. It is responsible for generating white blood cells and antibodies, helping the body eliminate pathogens, and playing a crucial role in fluid and nutrient distribution. However, due to trauma, surgery, radiotherapy, and lymphatic system diseases (such as lymphatic vessel injury, lymphedema, etc.), the lymphatic circulation is often severely damaged, resulting in blocked lymphatic return, which in turn leads to tissue edema, decreased immune function, and serious quality of life problems. Lymphedema is a typical manifestation of lymphatic system diseases. Patients often present with limb swelling, limited mobility, and local inflammation, and may even develop into infection or skin ulcers in severe cases. According to estimates by the World Health Organization, approximately 300 million people worldwide are troubled by such edema. Although the medical community has been searching for effective treatment methods for a long time, there is still no non-surgical or surgical method that can completely restore the normal limb morphology and function. In fact, physical therapy remains the preferred treatment as the main treatment method or as an adjuvant to surgical treatment. Surgical methods are divided into two categories: drainage and resection techniques. To drain lymphedema, attempts have been made to use pedicled flaps, myocutaneous flaps, omental transposition, and intestinal flaps, but they have gradually been phased out due to reasons such as infection risk, poor efficacy, large surgical trauma, impaired function and appearance, and poor drainage effect. Currently, minimally invasive lymphovenous anastomosis (node vein anastomosis, lymphovenous anastomosis) and lymphatic vessel end-to-end anastomosis have been applied. To bypass lymphatic obstruction, lymphatic and venous autotransplantation has also been applied. These microsurgical procedures can be performed when some peripheral lymphatic collecting ducts still remain unobstructed and can partially drain tissue edema fluid. Recently, there has also been a treatment method of transplanting a lymphatic valve with its own vascularization from the healthy inguinal lymph node area to the blocked axillary or inguinal area.

[0003] However, in cases of advanced lymphedema, all major lymphatic vessels have been blocked, and tissue fluid accumulates in the interstitial space, spontaneously forming irregularly shaped "lakes" and sometimes interconnected "blind channels". In these cases, the solution is to create artificial channels to drain the edematous fluid into unblocked areas so that the fluid can be absorbed. With the rapid development of tissue engineering and regenerative medicine, artificial lymphatic vessel technology mimics the structure and function of natural lymphatic vessels, preparing pipe structures with lymphatic drainage ability to replace or repair damaged lymphatic vessels in the human body, which is expected to fundamentally solve the problem of lymphatic reflux disorder and provide a new solution for the repair of lymphatic system injuries. In the prior art, "bead"-shaped alginate fibers encapsulating cells are prepared by coaxial electrospinning technology driven by Plateau-Rayleigh instability for artificial lymphatic vessels. In addition, clinically, autologous transplantation of skin flaps with lymph nodes is currently used to reconstruct lymphatic channels, but venous reflux may occur due to changes in the venous-lymphatic pressure gradient after surgery, which is likely to cause anastomotic blockage and lead to surgical failure. To sum up, there is an urgent clinical need for a highly biomimetic unidirectional flow-guiding artificial lymphatic vessel to achieve effective regeneration of lymphatic vessels. Summary of the Invention

[0004] The main object of the present invention is to provide a method for highly precisely controllable preparation of a highly biomimetic unidirectional flow-guiding artificial lymphatic vessel.

[0005] Another object of the present invention is to provide a highly biomimetic unidirectional flow-guiding artificial lymphatic vessel prepared by the method for highly precisely controllable preparation of a highly biomimetic unidirectional flow-guiding artificial lymphatic vessel.

[0006] To achieve the above invention objects, the present invention adopts the following technical solutions:

[0007] The present invention provides a method for highly precisely controllable preparation of a highly biomimetic unidirectional flow-guiding artificial lymphatic vessel, comprising the following steps:

[0008] (1) Determine the dimensional parameters of the target artificial lymphatic vessel, including the curvature of the upper arc surface of the mold, the overall height, the diameter of the lower bottom surface, the diameter of the upper bottom surface, the height of a single flow-guiding unit in the unidirectional flow-guiding groove structure, and the spacing between two adjacent flow-guiding units.

[0009] (2) Verify the unidirectional flow-guiding effect of the unidirectional flow-guiding groove structure of the target artificial lymphatic vessel in step (1) through the fluid-structure interaction module of physical field simulation software.

[0010] (3) Analyze and optimize the dimensional parameters in step (1) according to the unidirectional flow-guiding effect in step (2), and prepare a mold for the unidirectional flow-guiding groove structure according to the optimized dimensional parameters.

[0011] (4) Prepare a biodegradable biomaterial casting solution. Aspirate the solution with a syringe and squeeze it onto a smooth horizontal surface. Use a spreader to evenly spread the solution. Wait for the solvent to evaporate and the material to approach solidification. Quickly roll the working surface of the unidirectional flow channel structure mold described in step (3) against the material on the smooth horizontal surface to obtain a film with a unidirectional flow structure, and wait for the material to completely solidify;

[0012] (5) Cut off the excess material around the film that has solidified on the plane in step (4), and curl it into a tube with a diameter of 0.1 - 20 mm perpendicular to the flow direction, that is, a highly biomimetic unidirectional flow artificial lymphatic vessel is obtained.

[0013] Preferably, in step (2), the physical field simulation software is selected from one of COMSOL Multiphysics, ANSYS, and Fluent, and is optimized through the fluid-structure interaction module.

[0014] Preferably, in step (1), the determination of the size parameters and the preparation method of the artificial lymphatic vessel preparation mold include:

[0015] Step a: Determine the size of the mold base carrying the unidirectional flow unit. The base is a combination of a cuboid with length, width, and height of a, b, and c respectively and a bow-shaped cylinder with height a, chord length b, and bottom bow diameter of b - 4b. Its side is connected to the top surface of the cuboid, where a = 60 - 400 mm, b = 0.314 - 100 mm, c = 2 - 100 mm;

[0016] Step b: Determine the size of a single unidirectional flow unit. A single unidirectional flow unit is a frustum of a cone with its axis parallel and coincident with the direction of the arc length of the base and partially embedded in the base. The ratio of its height to the total length of the conduit is 0.01:1 - 1, the ratio of the diameter of the lower bottom surface to the inner diameter of the flow conduit is 1:2 - 100, the ratio of the diameter of the upper bottom surface to the diameter of the lower bottom surface is 1:4 - 20, the ratio of the distance between two laterally adjacent arranged flow units to the diameter of the lower bottom surface is 0.01:1 - 1, and the distance between two longitudinally adjacent arranged flow units is 1 / 3 - 0 of its height;

[0017] Step c: Calculate the size of a single flow unit according to the diameter of the artificial lymphatic vessel obtained after curling, and further calculate in combination with the lateral and longitudinal distances between adjacent flow units. Determine the number of unidirectional flow units according to the arc surface area of the unidirectional flow channel structure mold;

[0018] Step d: Design the minimum thickness of the mold to be 2 mm and the maximum thickness to be 2.314 - 102 mm;

[0019] Step e: Select one of COMSOL Multiphysics, ANSYS, and Fluent through physical field simulation software, and optimize it through the fluid-structure interaction module;

[0020] Step f: Based on the above steps a to d, select stainless steel 316L as the material, and use micron-level selective laser melting for the casting of the unidirectional flow guiding structure.

[0021] Preferably, in step (4), the preparation method of the biodegradable biomaterial casting solution includes: using one of biodegradable polyesters (BPE) as the solvent and one of biodegradable polyurethanes (BPU) as the solute for dissolution respectively, and then performing homogeneous mixing and dissolution to prepare a casting solution with a mass-volume concentration w / v of 8-12%, wherein the mass ratio of BPU to BPE in the casting solution is 0.1:0.1-100, and the biodegradable biomaterial casting solution is prepared by compounding and using BPU with a weight-average molecular weight of 80,000-200,000 and BPE with a weight-average molecular weight of 80,000-200,000.

[0022] Preferably, in step (4), the solvent selected for preparing the biodegradable biomaterial casting solution is selected from one or more of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), dichloromethane (DCM), dimethyl sulfoxide (DMSO), acetone (AC), chloroform (TCM), ethyl acetate (EA), N,N-dimethylformamide (DMF), N,N-dimethylacetamide (DMA), and tetrahydrofuran (THF), and is prepared by compounding according to a certain volume ratio.

[0023] Preferably, in step (4), the biodegradable biomaterial casting solution is sucked out through a syringe and extruded onto a smooth plane, and a coater is used to evenly spread the solution over an area of 0.1-60 cm 2 , and the solvent is allowed to volatilize. The volatilization time is controlled to be 5-60 min through a closed container. When the biodegradable biomaterial casting solution is close to solidification but still maintains slight fluidity, the working surface of the mold is quickly oriented towards the plane, and the material on the plane is rolled to obtain a film with a unidirectional flow guiding structure and a thickness of 20-900 μm. Finally, continue to ventilate until the material is completely solidified.

[0024] The present invention also provides a highly biomimetic unidirectional flow guiding artificial lymphatic vessel, which is prepared by the highly biomimetic unidirectional flow guiding artificial lymphatic vessel high-precision controllable preparation method described in any one of the foregoing.

[0025] The present invention also provides the application of the highly biomimetic unidirectional flow guiding artificial lymphatic vessel as or in the preparation of medical products for highly biomimetic unidirectional artificial flow guiding lymphatic vessels.

[0026] Preferably, the method for using the highly biomimetic unidirectional flow artificial lymphatic vessel includes: excising the damaged or blocked lymphatic vessel, connecting the flow guiding input end of the highly biomimetic unidirectional flow artificial lymphatic vessel to the lymph fluid outlet of the original system, connecting the flow guiding output end to the lymph fluid inlet of the original system, then suturing the incision. After the lymph fluid flows through and infiltrates the biomimetic flow guiding structure, it is then quickly drained to the other end, achieving the patency of the damaged lymphatic system in this area and fundamentally solving problems such as lymphedema.

[0027] More preferably, the damaged or blocked autologous lymphatic vessel is replaced with the highly biomimetic unidirectional flow artificial lymphatic vessel; wherein, the overall structure of the highly biomimetic unidirectional flow artificial lymphatic vessel is composed of multiple unidirectional flow structure units arranged according to a certain spacing rule. Each adjacent two unidirectional flow structure units have the unidirectional flow function. The designed unidirectional flow structure can also promote the adhesion, migration and proliferation of lymphatic endothelial cells, quickly form a lymphatic endothelium, maintain the patency of the lymphatic vessel, and the biodegradable material used degrades slowly with the regeneration of autologous tissue until it is replaced by autologous tissue, realizing the regeneration of the lymphatic vessel.

[0028] More preferably, the highly biomimetic unidirectional flow artificial lymphatic vessel has a special unidirectional flow structure, and its size and pattern are adjusted according to requirements, and it can temporarily replace the damaged lymphatic vessel to achieve the unidirectional flow of lymph fluid.

[0029] Compared with the prior art, the beneficial effects of the present invention are at least as follows:

[0030] 1. The present invention fills the blank of current artificial lymphatic vessels. The designed unidirectional flow structure can replace the original diseased lymphatic tissue to play a role and effectively guide the unidirectional and rapid passage of lymph fluid.

[0031] 2. In the present invention, the material for making the unidirectional flow artificial lymphatic vessel can select functional biodegradable biomaterials to realize the regeneration of lymphatic tissue after transplantation.

[0032] 3. In the present invention, the unidirectional flow structure and size can be adjusted according to the clinical transplantation requirements.

[0033] 4. The present invention realizes the preparation of the biomimetic unidirectional flow artificial lymphatic vessel, can replace and reconstruct the lymph fluid delivery system for damaged or blocked lymphatic parts, and can effectively treat diseases such as lymphedema and lymphatic, nerve, small blood vessel, biliary tract, and urethral injuries. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a three-dimensional image of the mold of the highly biomimetic unidirectional flow artificial lymphatic vessel in Example 1.

[0035] Figure 2 It is a three-dimensional schematic diagram of the whole membrane with unidirectional flow structure units in Example 1.

[0036] Figure 3 It is the front view of the unidirectional flow guiding structure unit in Example 1.

[0037] Figure 4 It is the simulation of the flow guiding effect of the highly bionic unidirectional flow guiding artificial lymphatic vessel in Example 1 by COMSOL Multiphysics 6.0 software.

[0038] Figure 5 It is the physical picture of the mold of the highly bionic unidirectional flow guiding artificial lymphatic vessel in Example 1.

[0039] Figure 6 It is the physical picture of the highly bionic unidirectional flow guiding artificial lymphatic vessel in Example 1. Specific implementation manners

[0040] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the described embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0041] Example 1

[0042] In this example, a unidirectional flow guiding artificial lymphatic vessel with an inner diameter of 1 mm and a length of 60 mm is designed. After unfolding, the film has a length of 60 mm, a width of 10.5 mm, and a thickness of 0.05 mm. The length of a single frustum-shaped unidirectional flow guiding unit on one side is 60 mm, the diameter of the lower bottom surface is 200 μm, the diameter of the upper bottom surface is 50 μm, the spacing between two adjacent unidirectional flow guiding units in the horizontal direction is 50 μm, and a total of 39 unidirectional flow guiding units are arranged in parallel along the length direction of the base. According to the above parameters, the corresponding mold is prepared.

[0043] Weigh 0.64 g of polycaprolactone (PCL, Mn = 80000) and 0.16 g of polyurethane (PU, Mn = 80000), dissolve them in 9 mL of 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) and 1 mL of dichloromethane (DCM) respectively, and perform homogeneous mixing (12 h) at room temperature to obtain a degradable biomaterial casting solution; perform the following operations in a fume hood. Use a 10 mL medical syringe without a needle to suck 2 mL of the solution, slowly extrude it in one direction on a smooth polytetrafluoroethylene plane with a length of 60 mm, and use a coater to evenly spread the solution with an area of 7.2 cm 2(60 mm × 12 mm), perform semi - enclosed covering above the solution to prevent the solvent from volatilizing too fast and causing the film to be too thick. Wait for the solvent to basically volatilize (30 min). When the solution still has low fluidity, use a mold to roll - press and imprint the unidirectional flow - guiding structure on it. Then continue to place it in the fume hood for 24 h to wait for the solvent to completely volatilize to form a film with a unidirectional flow - guiding structure. Use a sharp blade to cut off the excess part of the film without the unidirectional flow - guiding structure. Slowly tear the film along the width direction. Then attach the side with the unidirectional flow - guiding structure to a wire with a diameter of 1 mm and curl it along the width direction. When the film is curled into a tube shape, use a hair dryer to blow warm air for 3 s for short - term heat setting, and then take it off to obtain a highly biomimetic unidirectional flow - guiding artificial lymphatic vessel.

[0044] Example 2

[0045] In this example, a unidirectional flow - guiding artificial lymphatic vessel with an inner diameter of 1 mm and a length of 60 mm is designed. After unfolding, the film is 60 mm long, 10.5 mm wide, and 0.05 mm thick. The length of a single frustum - shaped unidirectional flow - guiding unit on one side is 0.6 mm, the diameter of the lower bottom surface is 200 μm, the diameter of the upper bottom surface is 50 μm, the spacing between two adjacent unidirectional flow - guiding units in the transverse direction is 50 μm, the spacing between two adjacent unidirectional flow - guiding units in the longitudinal direction is 0, and a total of 390 unidirectional flow - guiding units are arranged in parallel along the length direction of the base. Prepare the corresponding mold according to the above parameters.

[0046] Weigh 0.64 g of polycaprolactone (PCL, Mn = 80000) and 0.16 g of polyurethane (PU, Mn = 80000), dissolve them in 9 mL of HFIP and 1 mL of DCM respectively, and perform homogeneous mixing at room temperature (12 h) to obtain a biodegradable biomaterial casting solution; perform the following operations in the fume hood. Use a 10 - mL medical syringe without a needle to suck 2 mL of the solution and slowly extrude it in one direction on a smooth polytetrafluoroethylene plane with a length of 60 mm. Use a coater to evenly spread the solution with an area of 7.2 cm 2 (60 mm × 12 mm), perform semi - enclosed covering above the solution to prevent the solvent from volatilizing too fast and causing the film to be too thick. Wait for the solvent to basically volatilize (30 min). When the solution still has low fluidity, use a mold to roll - press and imprint the unidirectional flow - guiding structure on it. Then continue to place it in the fume hood for 24 h to wait for the solvent to completely volatilize to form a film with a unidirectional flow - guiding structure. Use a sharp blade to cut off the excess part of the film without the unidirectional flow - guiding structure. Slowly tear the film along the width direction. Then attach the side with the unidirectional flow - guiding structure to a wire with a diameter of 1 mm and curl it along the width direction. When the film is curled into a tube shape, use a hair dryer to blow warm air for 3 s for short - term heat setting, and then take it off to obtain a highly biomimetic unidirectional flow - guiding artificial lymphatic vessel.

[0047] Example 3

[0048] In this embodiment, a unidirectional flow artificial lymphatic vessel with an inner diameter of 1 mm and a length of 60 mm is designed. After unfolding, the film is 60 mm long, 10.5 mm wide, and 0.05 mm thick. The length of a single frustum-shaped unidirectional flow unit on one side is 60 mm, the diameter of the lower bottom surface is 200 μm, the diameter of the upper bottom surface is 50 μm, and the spacing between two adjacent unidirectional flow units in the transverse direction is 50 μm. A total of 39 unidirectional flow units are arranged in parallel along the length direction of the base, and a corresponding mold is prepared according to the above parameters.

[0049] Weigh 0.64 g of polycaprolactone (PCL, Mn = 80000) and 0.16 g of polyurethane (PU, Mn = 80000), dissolve them in 9 mL of HFIP and 1 mL of DCM respectively, and perform homogeneous mixing (12 h) at room temperature to obtain a degradable biomaterial casting solution. Conduct the following operations in a fume hood. Use a 10 mL medical syringe without a needle to draw 2 mL of the solution and slowly extrude it along one direction on the side of the mold with the target structure, allowing it to level naturally in the length and width directions. Cover it semi-closed above the solution to prevent the solvent from volatilizing too quickly and causing bubbles. Place it in the fume hood for 24 h to wait for the solvent to completely volatilize and the material to completely solidify on the surface of the mold, forming a film with a unidirectional flow structure. Use a sharp blade to cut off the excess material along the four sides of the mold, slowly tear the film along the width direction, then attach the side with the unidirectional flow structure to a 1 mm diameter iron wire and curl it along the width direction. Wait until the film is curled into a tube shape, use a hair dryer to blow warm air for 3 s for short-term heat setting, and then take it off to obtain a highly biomimetic unidirectional flow artificial lymphatic vessel.

[0050] Comparative Example 1

[0051] The difference between this comparative example and Example 1 is that no structure is adopted, and it is a tube with a smooth interior.

[0052] Comparative Example 2

[0053] The difference between this comparative example and Example 1 is that the frustum of the flow guiding unit structure has a length of 60 mm, a lower bottom surface diameter of 500 μm, and an upper bottom surface diameter of 25 μm.

[0054] Comparative Example 3

[0055] The difference between this comparative example and Example 1 is that the spacing between two adjacent flow guiding units in the transverse direction is 200 mm.

[0056] Comparative Example 4

[0057] The difference between this comparative example and Example 1 is that the degradable biomaterial solution is scraped onto the mold.

[0058] Comparative Example 5

[0059] The difference between this comparative example and Example 1 is that the mass-volume concentration of the biodegradable biomaterial solution is 12 wt%.

[0060] Comparative Example 6

[0061] The difference between this comparative example and Example 1 is that the mass ratio of BPE:BPU is 100:0.

[0062] Comparative Example 7

[0063] The difference between this comparative example and Example 1 is that the volume ratio of HFIP:DCM is 10:90.

[0064] Comparative Example 8

[0065] The difference between this comparative example and Example 1 is that the solvent evaporation time is controlled to be 5 min.

[0066] Comparative Example 9

[0067] The difference between this comparative example and Example 2 is that the distance between two adjacent longitudinal diversion units is 200 μm.

[0068] Performance Test

[0069] Artificial lymphatic vessels were respectively prepared from Examples 1 to 3 and Comparative Examples 1 to 8, and the horizontal unidirectional diversion ability test was carried out. The test method is as follows: Place the artificial lymphatic vessel vertically, with the diversion liquid input side facing down and immersed in the PBS solution of black ink, record the final stagnant height of the black solution, and record the final time used.

[0070] Table 1: Vertical unidirectional diversion liquid ability in the examples

[0071]

[0072]

[0073] As shown in Table 1, by strictly controlling the unidirectional diversion structure design, film preparation process, polymer solution concentration, polymer mass ratio, solvent selection, and solvent volume ratio, the highly biomimetic unidirectional diversion artificial lymphatic vessel has a good unidirectional diversion effect, effectively avoiding problems such as the failure of the unidirectional diversion effect caused by unreasonable diversion structure design, preparation process, too thin film, film surface roughness, ineffective unidirectional diversion structure, and damage to the unidirectional diversion structure.

[0074] The above are the preferred embodiments of the present invention, but the present invention should not be limited to the content disclosed in this embodiment. Therefore, all equivalent or modified implementations completed without departing from the spirit disclosed in the present invention fall within the protection scope of the present invention.

Claims

1. A high-precision controllable preparation method of a highly bionic one-way conduit artificial lymphatic vessel, characterized in that: The following steps are involved: (1) Determine the size parameters of the target artificial lymphatic tube, including the curvature and overall height of the upper arc surface of the mold, the upper bottom diameter, lower bottom diameter, and height of a single guide unit in the one-way guide structure, and the spacing between two adjacent guide units; (2) simulating the artificial lymphatic unidirectional flow channel structure in step (1) through the fluid-solid coupling module of the physical field simulation software to verify its unidirectional flow effect; (3) analyzing and optimizing the dimensional parameters in step (1) based on the results in step (2), and preparing a mold with a unidirectional flow guide groove structure according to the optimized dimensional parameters; (4) preparing a biodegradable biomaterial solution, drawing the solution through a syringe and squeezing it onto a smooth horizontal plane, spreading the solution evenly with an applicator, and when the solvent evaporates and the material is close to solidification, quickly rolling the material on the plane with the working surface of the mold to obtain a film with a unidirectional flow-conducting structure, and waiting for the material to be completely solidified; (5) The film coated on the plane and solidified in step (4) is trimmed of excess material around it, and is rolled perpendicularly to the flow direction to form a one-way flow guide tube with a diameter of 0.1 to 20 mm.

2. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 1 is characterized in that: In step (1), the method for designing the parameters of the artificial lymphatic vessel includes: Step a: Determine the size of the mold base equipped with the one-way guide unit. The base is a combination of a cuboid with a length, width, and height of a, b, and c respectively, and an arched column with a height of a, a chord length of b, and a bottom arched diameter of b to 4b, and its side surface is connected to the top surface of the cuboid, wherein a=60 to 400 mm, b=0.314 to 100 mm, and c=2 to 100 mm; Step b: Determine the size of a single one-way flow guide unit. The single one-way flow guide unit is a truncated cone whose central axis is parallel to and coincides with the direction of the arc surface length of the base and is partially embedded in the base. The ratio of its height to the total length of the conduit is 0.01:1 to 1:1, the ratio of the lower bottom surface diameter to the inner diameter of the flow guide tube is 1:2 to 1:100, the ratio of the upper bottom surface diameter to the lower bottom surface diameter is 1:4 to 1:20, the ratio of the spacing between two adjacent flow guide units arranged in the transverse direction to the lower bottom surface diameter is 0.01:1 to 1:1, and the spacing between two adjacent flow guide units arranged in the longitudinal direction is 0 to 1 / 3 of its height; Step c: Calculate the area of ​​the mold arc surface with the guide structure according to the diameter of the artificial lymphatic tube obtained after curling, and determine the number of unidirectional guide units; Step d: Design the mold thickness to be 2 mm at the minimum and 2.314 to 102 mm at the maximum; Step e: Based on the above steps a to d, stainless steel 316L is selected as the material, and micron-level selective laser melting is used to cast the unidirectional flow-guiding structure.

3. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 2 is characterized in that: In step (1), the size parameters of the artificial lymphatic tube include: the film with a unidirectional guide structure unit is 60 to 400 mm long and 0.314 to 100 mm wide, the length of a single unidirectional guide unit is 0.06 to 400 mm, the diameter of the lower bottom surface is 1 to 1000 μm, the diameter of the upper bottom surface is 0.25 to 250 μm, the spacing between two adjacent unidirectional guide units is 0.01 to 1 μm, the unidirectional guide units are arranged in parallel along the long direction of the base, and the diameter of the tube after being curled into a tube along the width direction is 0.1 to 20 mm.

4. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 1 is characterized in that: In step (2), the physical field simulation software is selected from one of COMSOL Multiphysics, ANSYS, and Fluent.

5. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 1, characterized in that: In step (2), the physical field simulation software uses a fluid-solid coupling module to perform simulation.

6. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 1, characterized in that: In step (4), the degradable biomaterial casting solution is prepared by dissolving one kind of degradable polyester (BPE) and one kind of degradable polyurethane (BPU) respectively, and then mixing and dissolving them homogeneously to prepare a casting solution with a mass-volume concentration w / v of 8 to 12% of the mixed solute and the mixed solvent, wherein the mass ratio of the solute BPU to the BPE in the mixed solution is 0.1:100 to 100:0.1; wherein the degradable biomaterial casting solution is selected from BPU with a weight average molecular weight of 80,000 to 200,000 and BPE with a weight average molecular weight of 80,000 to 200,000 for compounding, and the selected solvent is one or more of 1,1,1,3,3,3-hexafluoro-2-propanol, dichloromethane, dimethyl sulfoxide, acetone, chloroform, ethyl acetate, N,N-dimethylformamide, N,N-dimethylacetamide, and tetrahydrofuran.

7. The high-precision controllable preparation method of the highly biomimetic one-way conduit artificial lymphatic vessel according to claim 1, characterized in that: In step (4), the degradable biomaterial casting solution is sucked by a syringe and extruded onto a smooth surface, and the solution is evenly spread over an area of ​​0.1 to 60 cm using a spreader. 2 , cover the coating plane, control the solvent evaporation rate, wait for the solvent to evaporate for 5 to 60 minutes, when the degradable biomaterial casting solution is close to solidification but still maintains fine flowability, quickly turn the mold working surface toward the plane, roll the material on the plane to obtain a film with a unidirectional flow guide structure and a thickness of 20 to 900 μm, and continue ventilation until the material is completely solidified.

8. A highly bionic one-way conduit artificial lymphatic vessel, characterized in that: The artificial lymphatic vessel is prepared by the high-precision controllable preparation method of the highly bionic unidirectional conducting artificial lymphatic vessel according to any one of claims 1 to 7.

9. Use of the highly bionic unidirectional guiding artificial lymphatic vessel according to claim 8 as or in the preparation of a medical device product of the highly bionic unidirectional guiding artificial lymphatic vessel.

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