Incubator monitoring system for monitoring gas content in real time and working method thereof

Abstract

The invention relates to the technical field of microbial culture, in particular to an incubator monitoring system for monitoring gas content in real time and a working method thereof. The system comprises a gas environment monitoring module arranged in a culture tank of the incubator and a data receiving and displaying module arranged outside the incubator. The gas environment monitoring module is used for collecting environment information in the culture tank and converting the environment information into wireless signals to be sent. And the gas environment monitoring module analyzes the environment information. By adopting the system, various key environmental factor indexes in the culture process can be monitored, and the microorganism culture quality is relatively good.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial culture, and specifically to an incubator monitoring system for real-time monitoring of gas content and its working method. Background Art

[0002] According to the utilization and tolerance of microorganisms to oxygen, scientists classify microorganisms into aerobic, microaerophilic, and anaerobic microorganisms. Anaerobic and microaerophilic microorganisms cannot grow and reproduce under normal oxygen concentration conditions. For their culture and inspection, it is necessary to first form the anaerobic and microaerophilic environments they need in a culture tank. To obtain a relatively ideal anaerobic and microaerophilic microbial culture environment, the current economical and effective method is to use the vacuum gas replacement method. This method adjusts the oxygen concentration in the culture tank by replacing the gas in the culture tank with an anaerobic gas from the outside. Different microorganisms have different requirements for the oxygen concentration in the tank. The accuracy of the oxygen content in the culture tank and the stability of the gas environment during the culture process play a crucial role in the successful culture of microorganisms.

[0003] At present, when using the vacuum gas replacement method to set the anaerobic and microaerophilic microbial culture environment, only the volume of oxygen gas to be replaced in the culture tank is calculated theoretically. Then, the culture tank is placed in a constant temperature incubator for microbial culture. During the process of gas replacement to generate the gas environment required by microorganisms, the oxygen concentration in the culture tank cannot be accurately controlled, and at the same time, during the microbial culture process, it is impossible to monitor in real time whether there are changes or fluctuations in the key indicators affecting microbial culture, such as the oxygen and carbon dioxide content, temperature, humidity, and pressure in the tank. Since the key environmental factors during the culture process cannot be monitored in real time, the final quality of microbial culture cannot be guaranteed to be optimal. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to provide an incubator monitoring system for real-time monitoring of gas content. By using this system, various key environmental factor indicators during the culture process can be monitored, and the quality of microbial culture is relatively good.

[0005] To solve the above problems, the following solutions are provided:

[0006] The real-time gas content monitoring incubator monitoring system of the present invention is characterized by including

[0007] A gas environment monitoring module, which is arranged in the culture tank of the incubator, is used to collect the environmental information in the culture tank and convert the environmental information into a wireless signal for transmission;

[0008] A data receiving and displaying module disposed outside the incubator. The data receiving and displaying module includes a wireless and wired receiving unit and a main control unit, and the wireless and wired receiving unit is adaptively connected to the main control unit; the wireless and wired receiving unit is used to be directly or indirectly connected to the gas environment monitoring module; when the incubator is made of non-shielding material, the wireless and wired receiving unit is directly wirelessly connected to the gas environment monitoring module for receiving the wireless signal; when the incubator is made of shielding material, a signal transfer module is further included. The signal transfer module is disposed inside the incubator and outside the culture tank. The signal transfer module is wirelessly connected to the gas environment monitoring module and is wired-connected to the wireless and wired receiving unit. The signal transfer module is used to receive the wireless signal and send the wireless signal to the wireless and wired receiving unit by means of wired transmission.

[0009] The wireless and wired receiving unit sends the environmental information to the main control unit, and the main control unit analyzes the environmental information.

[0010] Among them, the gas environment monitoring module includes a first power supply unit, a sensor unit, a Bluetooth transmitter and a control unit; the first power supply unit is used to supply electrical energy to the sensor unit, the Bluetooth transmitter and the control unit; the sensor unit and the Bluetooth transmitter are adaptively connected to the control unit. The sensor unit is used to collect the environmental information in the culture tank and transmit the environmental information to the control unit, and the control unit forms a Bluetooth signal through the Bluetooth transmitter to realize wireless signal transmission.

[0011] The first power supply unit includes a battery, a battery charge and discharge management component and a voltage conversion component; the battery charge and discharge management component includes a first female socket, a battery management chip, a power switch chip and a P-type power tube Q1; the first female socket is adaptively connected to the battery management chip, and the first female socket is used to be connected to an external power supply to realize battery charging; the battery is connected to the source electrode of the P-type power tube Q1 through the battery management chip, the drain electrode of the P-type power tube Q1 is connected to the voltage conversion component, the voltage conversion component is connected to the sensor unit, the Bluetooth transmitter and the control unit, and the power switch chip is connected to the gate electrode of the P-type power tube Q1; the power switch chip controls the conduction or cut-off of the P-type power tube Q1 through a button. When the button is pressed, the P-type power tube Q1 is turned on, and the electrical energy of the battery enters the voltage conversion component through the battery management chip and the P-type power tube Q1. The voltage conversion component converts the voltage of the electrical energy released by the battery into the working voltage of the sensor unit, the Bluetooth transmitter and the control unit for the sensor unit, the Bluetooth transmitter and the control unit to use.

[0012] The gas environment monitoring module includes a first housing and a first circuit board; the first power supply unit, the sensor unit, the Bluetooth transmitter, and the control unit are all integrated on the first circuit board. There are air vents on the side wall of the first housing corresponding to the sensor unit, and the air vents are covered with waterproof and breathable membranes.

[0013] The gas environment monitoring module includes a battery voltage detection unit, and the battery voltage detection unit includes a PMOS transistor Q2 and an NMOS transistor Q3; the drain of the NMOS transistor Q3 is connected to one end of a resistor R21 and the gate of the PMOS transistor Q2. The source of the PMOS transistor Q2 and the other end of the resistor R21 are both connected to the positive electrode VBt of the battery. The gate of the NMOS transistor Q3 is connected to one end of a resistor R22. The source of the NMOS transistor Q3 and the other end of the resistor R22 are grounded. The gate of the NMOS transistor Q3 is connected to the control unit for receiving the voltage detection enable AinEN generated by the control unit; the drain of the PMOS transistor Q2 is connected to one end of a resistor R23. One end of the resistor R23 is connected to one end of a resistor R24, and the other end of the resistor R24 is grounded. A capacitor C6 is connected in parallel between the two ends of the resistor R24. One end of the resistor R23 connected to the resistor R24 is connected to the control unit, and the end of the resistor R23 connected to the resistor R24 forms a voltage signal Ain2.

[0014] The signal transfer module includes a second power supply unit, a first RS485 unit, a first Bluetooth receiver, and a controller; the second power supply unit is adaptively connected to the first RS485 unit, the first Bluetooth receiver, and the controller. The second power supply unit is used to provide electrical energy for the first RS485 unit, the first Bluetooth receiver, and the controller; the first Bluetooth receiver and the first RS485 unit are adaptively connected to the controller. When the incubator is made of shielding material, the first Bluetooth receiver is Bluetooth-connected to the Bluetooth transmitter. The first Bluetooth receiver is used to receive the Bluetooth signal formed by the environmental information through the Bluetooth transmitter and send the Bluetooth signal to the controller. The controller converts the Bluetooth signal into a wired signal and sends it to the first RS485 unit. The first RS485 unit is used to be wired-connected to the wireless and wired receiving unit.

[0015] The wired and wireless receiving unit includes a second RS485 unit, a serial port selection and switching unit, and a second Bluetooth receiver; the second RS485 unit is connected to the first RS485 unit, the second RS485 unit and the second Bluetooth receiver are adaptively connected to the serial port selection and switching unit, and the serial port selection and switching unit is adaptively connected to the main control unit; the main control unit is adaptively connected to the second RS485 unit, the serial port selection and switching unit, and the second Bluetooth receiver for controlling the second RS485 unit, the serial port selection and switching unit, and the second Bluetooth receiver; the second RS485 unit receives environmental information through wired transmission, and the second Bluetooth receiver receives the Bluetooth signal formed by the Bluetooth transmitter of the environmental information.

[0016] The working method includes a standard mode, a low power consumption mode, and a super power saving mode, which are specifically as follows:

[0017] Standard mode: After the gas environment monitoring module is started, all sensors in the sensor unit collect data every 1 - 2 seconds.

[0018] Low power consumption mode: After the gas environment monitoring module has been working properly in the standard mode for 2 - 2.5 hours and the gas environment in the culture tank is in a stable state, it enters the low power consumption mode; in the low power consumption mode, the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor in the sensor unit are started every 25 - 35 minutes, and the rest of the time is in the sleep state, and the pressure sensor is in the normal working state.

[0019] Super power saving mode: After the low power consumption mode exceeds 24 hours and the gas environment in the culture tank is in a stable state, the gas environment monitoring module enters the super power saving mode, and the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started every 60 minutes.

[0020] Among them, in the low power consumption mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started, they are stable for 8 - 10 minutes, and then 20 data are extracted within 2 minutes, and then enter the sleep state, and so on, until the low power consumption mode exceeds 24 hours and the gas environment in the culture tank is in a stable state.

[0021] In the super power saving mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started, they are stable for 8 - 10 minutes, and then 20 data are extracted within 2 minutes, and then enter the sleep state, and so on.

[0022] Adopting the above solutions has the following advantages:

[0023] 1. Since the incubator monitoring system for real-time monitoring of gas content in the present invention conducts environmental monitoring through the gas environment monitoring module disposed in the culture tank, when the incubator is made of non-shielding material, the wireless and wired receiving unit of the data receiving and displaying module disposed outside the incubator is directly wirelessly connected to the gas environment monitoring module. When the incubator is made of shielding material, the signal transfer module is disposed inside the incubator and outside the culture tank. The signal transfer module is wirelessly connected to the gas environment monitoring module and is wired-connected to the wireless and wired receiving unit. The signal transfer module is used to receive the environmental information transmitted wirelessly and send the environmental information to the wireless and wired receiving unit by means of wired transmission. The wireless and wired receiving unit sends the environmental information to the main control unit of the data receiving and displaying module, and the main control unit displays the environmental information through the display screen. This monitoring system can be applied to incubators made of different materials. Moreover, through the gas environment monitoring module and the data receiving and displaying module, the staff can obtain the key environmental factor indexes in the culture process, thereby ensuring that the quality of microorganism culture reaches the best.

[0024] 2. Three working modes are adopted to reduce the power consumption of the battery, so that the monitoring system can realize the monitoring of long-term cell culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is the structural topology diagram of the incubator monitoring system for real-time monitoring of gas content in the present invention.

[0026] Figure 2 is the structural topology diagram of the gas environment monitoring module in the incubator monitoring system for real-time monitoring of gas content in the present invention;

[0027] Figure 3 is the circuit schematic diagram of the sensor unit, Bluetooth transmitter and control unit in the incubator monitoring system for real-time monitoring of gas content in the present invention;

[0028] Figure 4 is the circuit schematic diagram of the first power supply unit in the incubator monitoring system for real-time monitoring of gas content in the present invention;

[0029] Figure 5 is the structural schematic diagram of the gas environment monitoring module in the incubator monitoring system for real-time monitoring of gas content in the present invention;

[0030] Figure 6 is Figure 5 the exploded schematic diagram of;

[0031] Figure 7 is the circuit schematic diagram of the battery voltage detection unit in the incubator monitoring system for real-time monitoring of gas content in the present invention;

[0032] Figure 8It is a circuit schematic diagram of the signal transfer module in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0033] Figure 9 It is a structural schematic diagram of the signal transfer module in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0034] Figure 10 It is a circuit schematic diagram of the wireless and wired receiving unit in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0035] Figure 11 The circuit schematic diagram of the main control unit in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0036] Figure 12 It is a circuit schematic diagram of the second female socket and the 5521 charging port in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0037] Figure 13 It is a circuit schematic diagram of the data storage unit, WIFI module and display screen in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0038] Figure 14 It is a structural schematic diagram of the data receiving and displaying module in the incubator monitoring system for real-time monitoring of gas content according to the present invention;

[0039] Figure 15 It is a working schematic diagram of the incubator monitoring system for real-time monitoring of gas content according to the present invention. Specific embodiments

[0040] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0041] Such as Figure 1 And Figure 15As shown in the figure, the incubator monitoring system for real-time monitoring of gas content according to the present invention includes a gas environment monitoring module disposed in the culture tank of the incubator and a data receiving and displaying module disposed outside the incubator. The gas environment monitoring module is used to collect the environmental information in the culture tank and convert the environmental information into a wireless signal for transmission. The data receiving and displaying module includes a wireless and wired receiving unit and a main control unit, and the wireless and wired receiving unit is adaptively connected to the main control unit. The wireless and wired receiving unit is used to be directly or indirectly connected to the gas environment monitoring module. When the incubator is made of non-shielding material, the wireless and wired receiving unit is directly wirelessly connected to the gas environment monitoring module for receiving wireless signals. When the incubator is made of shielding material, it further includes a signal transfer module disposed inside the incubator and outside the culture tank. The signal transfer module is wirelessly connected to the gas environment monitoring module and is wired-connected to the wireless and wired receiving unit. The signal transfer module is used to receive wireless signals and transmit the wireless signals to the wireless and wired receiving unit by means of wired transmission. The wireless and wired receiving unit sends the environmental information to the main control unit, and the main control unit analyzes the environmental information.

[0042] Specifically, the incubator monitoring system for real-time monitoring of gas content according to the present invention provides two connection and communication methods between the gas environment monitoring module and the data receiving and displaying module. Method 1: When the culture tank equipped with the gas environment monitoring module is placed in a constant temperature and humidity incubator made of non-shielding material (i.e., non-metallic material, such as plastics like ABS and PC), the gas environment monitoring module collects the gas environment information in the tank and realizes data transmission by wirelessly connecting to the data receiving and displaying module. Method 2: When the culture tank equipped with the gas environment monitoring module is placed in a constant temperature and humidity incubator made of shielding material (such as metal), since the wireless signal cannot directly penetrate the metal, at this time, the gas environment monitoring module collects the gas environment information in the tank and is wirelessly connected to the transfer module to realize data transmission. Then the signal transfer module is wired-connected to the data receiving and displaying module through a data line to transmit the data collected by the gas environment monitoring module to the data receiving and displaying module. This data line can be a flexible FPC line. Sealing rubber strips are provided between the door panel and the door frame of the incubator, which belongs to the prior art and will not be elaborated here. The data line passes through the sealing rubber strip between the door panel and the door frame and is connected to the data receiving and displaying module. The sealing rubber strip is flexible and pressing on the data line will not affect the sealing effect nor damage the data line.

[0043] Such as Figure 2As shown in the figure, the gas environment monitoring module includes a first power supply unit, a sensor unit, a Bluetooth transmitter, and a control unit. The first power supply unit is used to supply electrical energy to the sensor unit, the Bluetooth transmitter, and the control unit. The sensor unit and the Bluetooth transmitter are adaptively connected to the control unit. The sensor unit is used to collect the environmental information inside the culture tank and transmit the environmental information to the control unit. The control unit forms a Bluetooth signal through the Bluetooth transmitter with the environmental information and realizes wireless signal transmission.

[0044] As Figure 3 shown in the figure, in this embodiment, the control unit includes an MCU chip U6 of model STM32G070C8T6. The Bluetooth transmitter includes a chip BT1 of model E104-BT53A1_A3. The sensor unit includes a chip U1 of model LPS22, a chip U4 of model SCD4x, and a chip U2 of model LuminOx. The chip U1 integrates a pressure sensor, a temperature sensor, and a humidity sensor. The chip U4 is a carbon dioxide concentration sensor, and the chip U2 is an oxygen concentration sensor. The outer circuits and connection methods of the MCU chip U6, the chip BT1, the chip U1, the chip U4, and the chip U2 are as Figure 3 shown in the figure.

[0045] As Figure 4 shown in the figure, the first power supply unit includes a battery, a battery charge and discharge management component, and a voltage conversion component. The battery charge and discharge management component includes a first female socket, a battery management chip, a power switch chip, and a P-type power transistor Q1. The first female socket is adaptively connected to the battery management chip. The first female socket is used to connect to an external power supply to realize battery charging. The battery is connected to the source electrode of the P-type power transistor Q1 through the battery management chip. The drain electrode of the P-type power transistor Q1 is connected to the voltage conversion component. The voltage conversion component is connected to the sensor unit, the Bluetooth transmitter, and the control unit. The power switch chip is connected to the gate electrode of the P-type power transistor Q1. The power switch chip controls the conduction or cutoff of the P-type power transistor Q1 through a button. When the button is pressed, the P-type power transistor Q1 conducts, and the electrical energy of the battery enters the voltage conversion component through the battery management chip and the P-type power transistor Q1. The voltage conversion component converts the voltage of the electrical energy released by the battery into the working voltages of the sensor unit, the Bluetooth transmitter, and the control unit for the sensor unit, the Bluetooth transmitter, and the control unit to use. The gas environment monitoring module includes a first housing 101 and a first circuit board 105. The first power supply unit, the sensor unit, the Bluetooth transmitter, and the control unit are all integrated on the first circuit board 105. There are air vents 102 on the side wall of the first housing 101 corresponding to the sensor unit. The air vents 102 are covered with a waterproof and breathable membrane 104.

[0046] As Figure 4As shown, in this embodiment, the first female socket is a TYPE-C female socket. The battery management chip uses a chip U5 with the model number LGS5500, and the power switch chip uses a chip U7 with the model number EJ2130E. The connection methods of the TYPE-C female socket, chip U5, and chip U7 are as Figure 5 shown. The pin of chip U7 is connected to the button through the plug connector P3. Chip U7 and chip U6 are also connected to the three-color ring 103 lamp through the plug connector P3. The three-color ring 103 lamp contains LED_R (red), LED_B (blue), and LED_G (green). The connection methods of LED_R, LED_B, and LED_G with chip U7 and chip U6 are as Figure 4 and Figure 3 shown. The voltage conversion component contains a chip U8 with the model number XT3406 and a chip U9 with the model number GD25Q128, which are used to generate 5V power and VDD power. The specific structure is as Figure 5 shown. The connection methods of the voltage conversion component with the sensor unit, Bluetooth transmitter, and control unit are as Figure 3 and Figure 4 shown.

[0047] Specifically, the five sensors included in the sensor unit are used to collect various key data in the gas environment inside the culture tank. The battery of the gas environment monitoring module can be charged through the first female socket, ensuring that the gas environment monitoring module can continuously work in the sealed culture tank. The Bluetooth transmitter is integrated on the first circuit board 105, which is used to communicate with the Bluetooth receiver in the signal relay module or data receiving and display module to achieve data transmission. At the same time, the light control module is also integrated on the first circuit board 105. The working state of the gas environment monitoring module is visually displayed by controlling the light color of the three-color ring 103 lamp. When working normally, the three-color ring 103 lamp is green; in the charging state, that is, when the gas environment monitoring module is connected to the type-c charger, the three-color ring 103 lamp is red; in the Bluetooth pairing state, the three-color ring 103 lamp is yellow. When the Bluetooth pairing fails and the gas environment monitoring module is not connected to the type-c charger, the three-color ring 103 lamp is red. The specific colors can be selected according to the actual situation, which belongs to the prior art and will not be elaborated here.

[0048] As Figure 5 and Figure 6As shown in the figure, in this embodiment, the first housing 101 is wrapped around the outside of the first circuit board 105. Micro holes are arranged on the first housing 101 corresponding to the sensor unit to facilitate the sensor to collect the gas environment information in the culture tank. At the same time, it can also prevent large-particle foreign objects from entering the gas environment monitoring module and contaminating the sensors on the first circuit board 105. The ventilation holes 102 are micro holes, and a waterproof and breathable membrane 104 is covered on the micro holes. The waterproof and breathable membrane 104 mainly prevents the water vapor generated during the generation of an anaerobic or microaerophilic environment in the culture tank from entering the gas environment monitoring module and damaging the electronic components and sensors on the first circuit board 105. The waterproof and breathable membrane 104 can only block liquid water and will not block gaseous water, thus not affecting the detection results of the humidity sensor. The battery is protected by an aluminum shell 106 to prevent the battery from bulging due to pressure changes during the gas replacement process of the gas environment monitoring module in the culture tank. The three-color ring 103 is installed on the first housing.

[0049] As Figure 7 shown, the gas environment monitoring module includes a battery voltage detection unit, and the battery voltage detection unit includes a PMOS transistor Q2 and an NMOS transistor Q3. The drain of the NMOS transistor Q3 is connected to one end of a resistor R21 and the gate of the PMOS transistor Q2. The source of the PMOS transistor Q2 and the other end of the resistor R21 are both connected to the positive electrode VBt of the battery. The gate of the NMOS transistor Q3 is connected to one end of a resistor R22. The source of the NMOS transistor Q3 and the other end of the resistor R22 are grounded. The gate of the NMOS transistor Q3 is connected to the control unit for receiving the voltage detection enable AinEN generated by the control unit. The drain of the PMOS transistor Q2 is connected to one end of a resistor R23. One end of the resistor R23 is connected to one end of a resistor R24. The other end of the resistor R24 is grounded. A capacitor C6 is connected in parallel between both ends of the resistor R24. One end of the resistor R23 connected to the resistor R24 is connected to the control unit. The voltage signal Ain2 is formed at one end of the resistor R23 connected to the resistor R24. The connection method between the gas environment monitoring module and the MCU chip U6 is as Figure 7 and Figure 3 shown. The battery voltage detection unit can be used to detect the battery voltage, so as to issue an alarm when the battery power is low.

[0050] As Figure 8As shown, the signal relay module includes a second power supply unit, a first RS485 unit, a first Bluetooth receiver, and a controller. The second power supply unit is adaptively connected to the first RS485 unit, the first Bluetooth receiver, and the controller, and the second power supply unit is used to provide electrical energy for the first RS485 unit, the first Bluetooth receiver, and the controller. The first Bluetooth receiver and the first RS485 unit are adaptively connected to the controller. When the incubator is made of shielding material, the first Bluetooth receiver is Bluetooth-connected to the Bluetooth transmitter. The first Bluetooth receiver is used to receive the Bluetooth signal formed by the environmental information through the Bluetooth transmitter and send the Bluetooth signal to the controller. The controller converts the Bluetooth signal into a wired signal and sends it to the first RS485 unit. The first RS485 unit is used to be wired-connected to the wireless and wired receiving unit. Specifically, the second power supply unit includes a chip U10 of model XT3406, the first RS485 unit includes a chip U11 of model SN65HVD72D, the first Bluetooth receiver includes a chip BT2 of model E104-BT53A1_A3, and the controller includes a chip U12 of model STM32G030F6P6. The connection methods of the chip U10, the chip U11, the chip BT2, and the chip U12 are as Figure 8 shown. The 5V power supply of the chip U10 is provided by the data line of the data receiving and displaying module.

[0051] As Figure 9 shown, the signal relay module includes a second housing 201 and a second main board 202. The second power supply unit, the first RS485 unit, the first Bluetooth receiver, and the controller are all integrated on the second main board 202. The first Bluetooth receiver can be connected to the Bluetooth transmitter in the gas environment monitoring module. Receive the data collected by each sensor of the gas environment monitoring module, and the Bluetooth signal converter converts the relevant received gas environment data in the culture tank into wired data and transmits it to the data receiving and displaying module through the data line.

[0052] The wireless and wired receiving unit includes a second RS485 unit, a serial port selection and switching unit, and a second Bluetooth receiver. The second RS485 unit is connected to the first RS485 unit. The second RS485 unit and the second Bluetooth receiver are adaptively connected to the serial port selection and switching unit, and the serial port selection and switching unit is adaptively connected to the main control unit. The main control unit is adaptively connected to the second RS485 unit, the serial port selection and switching unit, and the second Bluetooth receiver for controlling the second RS485 unit, the serial port selection and switching unit, and the second Bluetooth receiver. The second RS485 unit receives environmental information through wired transmission, and the second Bluetooth receiver receives the Bluetooth signal formed by the environmental information through the Bluetooth transmitter.

[0053] As Figure 10 Figure 11As shown, the main control unit contains a chip U13 of model STM32G070C8T6, the second RS485 unit contains a chip U14 of model SN65HVD72D, the serial port selection and switching unit contains a chip U15 of model SN74CBT3257D, the second Bluetooth receiver contains a chip BT3 of model E104-BT53A1_A3, and the connection methods of chip U13, chip U14, chip U15 and chip BT3 are as Figure 10 and Figure 11 shown. The connection methods of the signal transfer module and the data reception and display module are as Figure 11 and Figure 8 shown.

[0054] In this embodiment, as Figure 12 and 13 shown, the data reception and display module contains a second female socket, the second female socket is a type-c female socket, a data storage unit, a 5521 charging port and a WIFI module. The second female socket is mainly used to connect to a laboratory computer or printer, and the data in the data storage unit can be exported to an external device for data analysis and traceability. The WIFI module enables the device to access the Internet through WiFi to achieve remote control and data exchange. The 5521 charging port cooperates with an external 5521 charger to supply power for the normal operation of the data reception and display module. The display screen 302 is mainly used to display the data related to the gas environment in the culture tank in real time, so as to facilitate the experimenter to understand the culture status of the microorganisms in the culture tank.

[0055] As Figure 14 shown, the data reception and display module contains a third housing 301 and a third main board 303. The wireless and wired reception unit, the main control unit, the second female socket, the data storage unit, the 5521 charging port and the WIFI module are all integrated on the third main board 303. The third main board 303 is located inside the third housing 301, and the display screen 302 is located on the side of the third housing 301. A magnet 304 can be fixedly arranged on the housing, so that the data reception and display module can be fixed on the metal outer wall of the incubator by suction, which is convenient for the experimenter to observe the culture situation of the microorganisms in the incubator and operate. A buzzer can also be arranged on the third main board 303 for alarming when the gas environment in the culture tank exceeds the set specifications.

[0056] The main control unit analyzes the environmental information by converting it into a value and displaying it on the display screen 302, comparing it with the pre-reviewed threshold value stored in it, and controlling the buzzer to give an alarm when it exceeds the normal range.

[0057] The working method of the incubator monitoring system for real-time monitoring of gas content in this embodiment includes a standard mode, a low power consumption mode and a super power saving mode, which are specifically as follows:

[0058] In the standard mode, after the gas environment monitoring module is started, all sensors in the sensor unit collect data every 1 - 2 seconds.

[0059] In the low - power mode, after the gas environment monitoring module has been working properly in the standard mode for 2 - 2.5 hours and the gas environment in the culture tank is in a stable state, it enters the low - power mode. In the low - power mode, the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor in the sensor unit are started every 25 - 35 minutes, and the rest of the time they are in the sleep state, while the pressure sensor is in the normal working state.

[0060] In the ultra - power - saving mode, after the low - power mode has exceeded 24 hours and the gas environment in the culture tank is in a stable state, the gas environment monitoring module enters the ultra - power - saving mode, and the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started every 60 minutes.

[0061] In the low - power mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started, after stabilizing for 8 - 10 minutes, 20 data are extracted within 2 minutes, and then they enter the sleep state, and so on, until the low - power mode has exceeded 24 hours and the gas environment in the culture tank is in a stable state.

[0062] In the ultra - power - saving mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor, and humidity sensor are started, after stabilizing for 8 - 10 minutes, 20 data are extracted within 2 minutes, and then they enter the sleep state, and so on, so as to ensure that the battery has enough electrical energy to realize environmental monitoring.

[0063] The gas environment monitoring module placed in the sealed culture tank in the present invention can transmit data to the data receiving and display module outside the incubator in wired and wireless ways. The data receiving and display module can be connected to and communicate with multiple gas monitoring modules simultaneously. Also, the gas environment monitoring module has multiple monitoring modes, corresponding to different power consumption requirements, to meet the short - cycle culture data monitoring and long - cycle culture data monitoring of customers. Moreover, the microbial gas content monitoring system can access the Internet through WiFi to realize remote control and data exchange.

[0064] This solution can accurately detect the gas environment data in the culture tank that affects the growth of microorganisms. It can monitor the gas environment data in the culture tank in real - time. The relevant monitoring data can be displayed and saved in real - time, which is convenient for subsequent traceability and processing analysis of the relevant environmental data.

Claims

1. A real-time monitoring system for incubator gas content, characterized in that: include, A gas environment monitoring module is arranged in the culture tank of the incubator, and is used to collect environmental information in the culture tank and convert the environmental information into wireless signals for transmission; A data receiving and displaying module is arranged outside the incubator, the data receiving and displaying module contains a wireless wired receiving unit and a main control unit, the wireless wired receiving unit is adaptively connected to the main control unit; the wireless wired receiving unit is used to be directly or indirectly connected to the gas environment monitoring module; when the incubator is made of non-shielded material, the wireless wired receiving unit is directly wirelessly connected to the gas environment monitoring module for receiving the wireless signal; when the incubator is made of shielded material, it also includes a signal relay module, the signal relay module is arranged inside the incubator and outside the incubator, the signal relay module is wirelessly connected to the gas environment monitoring module and wiredly connected to the wireless wired receiving unit, the signal relay module is used to receive the wireless signal and send the wireless signal to the wireless wired receiving unit by wired transmission; The wireless and wired receiving units send the environmental information to the main control unit, and the main control unit parses the environmental information.

2. The incubator monitoring system for real-time monitoring of gas content according to claim 2, characterized in that: The gas environment monitoring module contains a first power supply unit, a sensor unit, a Bluetooth transmitter and a control unit; the first power supply unit is used to provide power to the sensor unit, the Bluetooth transmitter and the control unit; the sensor unit and the Bluetooth transmitter are adapted to be connected with the control unit, the sensor unit is used to collect environmental information in the culture tank and transmit the environmental information to the control unit, and the control unit converts the environmental information into a Bluetooth signal through the Bluetooth transmitter to realize wireless signal transmission.

3. The incubator monitoring system for real-time monitoring of gas content according to claim 2, characterized in that: The first power supply unit includes a battery, a battery charge and discharge management component and a voltage conversion component; the battery charge and discharge management component includes a first mother socket, a battery management chip, a power switch chip and a P-type power tube Q1; the first mother socket is adaptably connected to the battery management chip, and the first mother socket is used to connect to an external power supply to realize battery charging; the battery is connected to the source of the P-type power tube Q1 through the battery management chip, the drain of the P-type power tube Q1 is connected to the voltage conversion component, the voltage conversion component is connected to the sensor unit, the Bluetooth transmitter and the control unit, and the power switch chip is connected to the gate of the P-type power tube Q1; the power switch chip controls the P-type power tube Q1 to be turned on or off through a button, and when the button is pressed, the P-type power tube Q1 is turned on, and the battery's electric energy enters the voltage conversion component through the battery management chip and the P-type power tube Q1, and the voltage conversion component converts the electric energy voltage released by the battery into the working voltage of the sensor unit, the Bluetooth transmitter and the control unit for use by the sensor unit, the Bluetooth transmitter and the control unit.

4. The incubator monitoring system for real-time monitoring of gas content according to claim 2, characterized in that: The gas environment monitoring module comprises a first shell and a first circuit board; the first power supply unit, the sensor unit, the Bluetooth transmitter and the control unit are all integrated on the first circuit board, and the side wall of the first shell corresponding to the sensor unit is provided with an air hole, and the air hole is covered with a waterproof breathable membrane.

5. The incubator monitoring system for real-time monitoring of gas content according to claim 2, characterized in that: The gas environment monitoring module includes a battery voltage detection unit, which includes a PMOS tube Q2 and an NMOS tube Q3; the drain of the NMOS tube Q3 is connected to one end of the resistor R21 and the gate of the PMOS tube Q2, the source of the PMOS tube Q2 and the other end of the resistor R21 are both connected to the positive electrode VBt of the battery, the gate of the NMOS tube Q3 is connected to one end of the resistor R22, the source of the NMOS tube Q3 and the other end of the resistor R22 are grounded, and the gate of the NMOS tube Q3 is connected to the control unit for receiving the voltage detection enable AinEN generated by the control unit; the drain of the PMOS tube Q2 is connected to one end of the resistor R23, the other end of the resistor R23 is connected to one end of the resistor R24, the other end of the resistor R24 ​​is grounded, a capacitor C6 is connected in parallel between the two ends of the resistor R24, one end of the resistor R23 connected to the resistor R24 ​​is connected to the control unit, and one end of the resistor R23 connected to the resistor R24 ​​forms a voltage signal Ain2.

6. The incubator monitoring system for real-time monitoring of gas content according to claim 2, characterized in that: The signal transfer module contains a second power supply unit, a first RS485 unit, a first Bluetooth receiver and a controller; the second power supply unit is adaptably connected to the first RS485 unit, the first Bluetooth receiver and the controller, and the second power supply unit is used to provide power to the first RS485 unit, the first Bluetooth receiver and the controller; the first Bluetooth receiver and the first RS485 unit are adaptably connected to the controller, and when the incubator is made of shielding material, the first Bluetooth receiver is connected to the Bluetooth transmitter by Bluetooth, the first Bluetooth receiver is used to receive the Bluetooth signal formed by the environmental information through the Bluetooth transmitter, and send the Bluetooth signal to the controller, the controller converts the Bluetooth signal into a wired signal and sends it to the first RS485 unit, and the first RS485 unit is used to be wiredly connected to the wireless wired receiving unit.

7. The incubator monitoring system for real-time monitoring of gas content according to claim 6, characterized in that: The wireless and wired receiving unit contains a second RS485 unit, a serial port selection switching unit and a second Bluetooth receiver; the second RS485 unit is connected to the first RS485 unit, the second RS485 unit and the second Bluetooth receiver are adaptively connected to the serial port selection switching unit, and the serial port selection switching unit is adaptively connected to the main control unit; the main control unit is adaptively connected to the second RS485 unit, the serial port selection switching unit and the second Bluetooth receiver, and is used to control the second RS485 unit, the serial port selection switching unit and the second Bluetooth receiver; the second RS485 unit receives environmental information through wired transmission, and the second Bluetooth receiver receives the Bluetooth signal formed by the environmental information through the Bluetooth transmitter.

8. The method of operating the incubator monitoring system for real-time monitoring of gas content according to claim 7, characterized in that: The working method includes standard mode, low power mode and super power saving mode, which are as follows: In standard mode, after the gas environment monitoring module is started, all sensors in the sensor unit collect data every 1-2 seconds; In low power mode, the gas environment monitoring module enters low power mode after it has been working normally for 2-2.5 hours in standard mode and the gas environment in the culture tank is in a stable state; in low power mode, the oxygen sensor, carbon dioxide sensor, temperature sensor and humidity sensor in the sensor unit are activated every 25-35 minutes, and are in dormant state for the rest of the time, and the pressure sensor is in normal working state; Super power saving mode: After more than 24 hours in low power mode and the gas environment in the culture tank is in a stable state, the gas environment monitoring module enters the super power saving mode, and the oxygen sensor, carbon dioxide sensor, temperature sensor and humidity sensor are started every 60 minutes.

9. The method for operating the incubator monitoring system for real-time monitoring of gas content according to claim 8, characterized in that: In low power mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor and humidity sensor are started, they stabilize for 8-10 minutes, extract 20 data within 2 minutes, and then enter the sleep state, and repeat this process until the low power mode exceeds 24 hours and the gas environment in the culture tank is in a stable state.

10. The working method of the incubator monitoring system for real-time monitoring of gas content according to claim 8, characterized in that: In super power saving mode, after the oxygen sensor, carbon dioxide sensor, temperature sensor and humidity sensor are started, they stabilize for 8-10 minutes, extract 20 data in 2 minutes, and then enter the sleep state, and repeat this process.

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