An automated cell detection device

Abstract

The present invention relates to the field of detection technology, and discloses an automated cell detection device, comprising a base, and further comprising: a turntable, which is rotatably arranged above the base, and has four rectangular grooves evenly distributed in a ring shape on the top of the turntable; a supporting assembly, which has four supporting assemblies, which are distributed in a ring shape on the turntable, and are used to support cells to be detected; and a liquid extraction and cleaning assembly, which is arranged on the base, and is used to clean the supporting assembly. The present invention, through the supporting assembly, the liquid extraction and cleaning assembly, can automatically extract liquid from the liquid extraction box by rotating the turntable, and the liquid extraction plate can automatically move back and forth, so that the liquid extraction plate can completely extract liquid from the cover glass and the slide glass, and complete the comprehensive cleaning of the cover glass and the slide glass, without the need to frequently replace the cover glass and the slide glass. In addition, after the cleaning is completed, the corresponding liquid extraction plate can be automatically flipped over, thereby ensuring the neatness of the entire device.

Description

Technical Field

[0001] The present invention relates to the field of detection technology, and in particular to an automated cell detection device. Background Art

[0002] Automated cell testing uses advanced technology and equipment to efficiently and accurately detect and analyze cells. This technology is widely used in biomedical research, clinical diagnosis, drug development and other fields. Its main goal is to improve detection speed, accuracy and repeatability, reduce errors caused by manual operations, and provide reliable data support for related research.

[0003] Although the current automated cell detection device can automatically detect cells, it does not have the function of cleaning the slides and coverslips after detecting cells, resulting in the need to frequently replace the slides and coverslips. Replacing the slides and coverslips wastes a lot of time and reduces the efficiency of cell detection. Therefore, there is an urgent need to design an automated cell detection device. Summary of the Invention

[0004] The purpose of the present invention is to solve the problems existing in the prior art and to propose an automated cell detection device.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] An automated cell detection device, comprising a base and:

[0007] The turntable is rotatably arranged above the base, and the top of the turntable is provided with four rectangular grooves evenly distributed in a circular shape;

[0008] There are four carrying components, which are distributed in a ring shape on the turntable and are used to carry cells to be tested;

[0009] A liquid extraction and cleaning component is provided on the base and is used to clean the carrying component;

[0010] The pump cleaning kit includes:

[0011] There are two liquid extraction plates, and the corresponding sides of the two liquid extraction plates are each provided with a liquid extraction port;

[0012] A flipping mechanism, which is used to flip the corresponding liquid extraction plate;

[0013] A liquid extraction mechanism, the liquid extraction mechanism is used to enable the liquid extraction plate to extract the cell fluid on the carrying component;

[0014] The driving assembly is arranged on the top of the base, and the driving assembly is used to make the turntable, the carrying assembly and the liquid extraction and cleaning assembly work.

[0015] As a further technical solution of the present invention, a bracket is vertically mounted on the top of the base, and an electron microscope is mounted on the bottom of the horizontal side of the bracket;

[0016] A storage box is also vertically installed on the top of the base, and a liquid pump connected to the inside of the storage box is installed on the top of the storage box, and a U-shaped pipe is installed on the liquid pump;

[0017] An arc-shaped rack is slidably provided on the top of the turntable, and a mounting rod is fixed on the top of the arc-shaped rack, and the top end of the mounting rod is fixedly mounted on the top of the bracket.

[0018] As a further technical solution of the present invention, the bearing assembly includes:

[0019] Slide, the slide is installed horizontally inside the rectangular groove;

[0020] A cover glass is horizontally arranged above the slide, and an outer frame is installed around the cover glass;

[0021] There are two limit rods, both of which are vertically fixed on the top of the turntable, and the surfaces of the limit rods are slidably arranged inside the outer frame of the cover glass;

[0022] A reciprocating screw is mounted on the top of the turntable for vertical rotation, and the surface of the reciprocating screw is connected to the inside of the cover glass frame through a thread;

[0023] The first gear is fixedly sleeved on the surface of the reciprocating screw and is meshed with the arc-shaped rack.

[0024] As a further technical solution of the present invention, the drive assembly includes:

[0025] A U-shaped frame is fixedly mounted on the top of the base, and a first column fixed to the center of the bottom of the turntable is vertically mounted on the top of the horizontal side of the U-shaped frame. A drive motor is vertically mounted on the top of the base, and the output shaft of the drive motor is mounted on the bottom end of the first column;

[0026] The missing gear is fixedly sleeved on the surface of the first column, and the convex teeth on the missing gear are divided into four groups, and the four groups of convex teeth are distributed at equal intervals;

[0027] The second column is vertically rotatably mounted on the top of the horizontal side of the U-shaped frame, and a one-way bearing is sleeved on the surface of the second column, and a small gear is fixedly sleeved on the outer ring of the one-way bearing, and the small gear is meshed with the missing gear;

[0028] The torsion spring is sleeved on the surface of the second column, and the two ends of the torsion spring are respectively fixedly installed on the bottom of the pinion and the top of the horizontal side of the U-shaped frame.

[0029] As a further technical solution of the present invention, a third column is vertically rotatably installed on the top of the horizontal side of the U-shaped frame, and a large gear is fixedly sleeved on the surface of the third column. The large gear and the small gear are meshed, and a first bevel gear is also fixedly sleeved on the surface of the third column.

[0030] As a further technical solution of the present invention, the liquid pumping mechanism includes:

[0031] The liquid pumping box is vertically installed on the top of the base, and a first turntable is rotatably installed above the liquid pumping box;

[0032] The second belt is rotatably arranged on the side of the liquid extraction box, and second pulleys are provided at both ends of the second belt;

[0033] One of the second pulleys is fixedly installed at the center of the first turntable end face through a connecting shaft, and the other second pulley end face is horizontally fixed with a rotating rod rotatably installed on the top of the base, and the other end of the rotating rod is fixed with a second bevel gear, and the first bevel gear and the second bevel gear are meshed.

[0034] As a further technical solution of the present invention, the liquid pumping mechanism further includes:

[0035] The piston plate is slidably arranged inside the liquid pumping box, and a piston rod is vertically fixed to the top of the piston plate. The top end of the piston rod passes through the liquid pumping box, and the top end of the piston rod rotates to rotate a first movable rod mounted on the outer edge of the side of the first turntable;

[0036] The liquid inlet pipe is connected and installed on the top of the liquid pumping box, and the vertical side of the liquid inlet pipe extends into the interior of the liquid pumping box. The surface of the liquid inlet pipe is slidably arranged inside the piston plate, and a first one-way valve is installed on the liquid inlet pipe;

[0037] The liquid outlet pipe is connected and installed on the top of the liquid pumping box, and the vertical side of the liquid outlet pipe extends into the interior of the liquid pumping box. The surface of the liquid outlet pipe is slidably arranged inside the piston plate, and a second one-way valve is installed on the liquid outlet pipe;

[0038] The liquid extraction pipe, the horizontal side of the liquid extraction pipe is slidably installed inside the liquid inlet pipe through a spline, and the liquid extraction pipe is a three-way pipe, and the other two ends of the liquid extraction pipe are respectively rotated and connected to the end faces of the two liquid extraction plates;

[0039] The collecting box is vertically installed on the top of the base, and the end of the liquid outlet pipe is located inside the collecting box.

[0040] As a further technical solution of the present invention, a support column is vertically rotatably mounted on the top of the base, and a second turntable is rotatably mounted on the top of the support column, a first pulley is fixedly sleeved on the surface of the support column and the surface of the second column, and a first belt is sleeved on the surfaces of the two first pulleys;

[0041] A second movable rod is rotatably mounted on the outer edge of the top of the second turntable, and the other end of the second movable rod is rotatably mounted on the side surface of the liquid extraction plate located above.

[0042] As a further technical solution of the present invention, the flipping mechanism includes:

[0043] The vertical plate is fixed vertically on the top of the base, and a telescopic rod is fixed horizontally on the top of the side of the vertical plate. The fixed end and the telescopic end of the telescopic rod are connected by a spline sliding connection;

[0044] The connecting block is fixedly installed on the top of the telescopic end of the telescopic rod, and two rotating shafts are provided on the side of the connecting block. The upper rotating shaft is fixedly installed between the connecting block and the liquid extraction plate, and the two ends of the rotating shaft located below rotate on the side of the connecting block and are fixed on the end face of the liquid extraction plate respectively.

[0045] As a further technical solution of the present invention, a second gear is fixedly sleeved on the surface of the rotating shaft below, and a spur rack is horizontally fixedly installed on the side of the vertical plate. When the spur rack contacts the second gear, the spur rack and the second gear are engaged.

[0046] The present invention, through the carrying component, the liquid extraction and cleaning component and the driving component, can automatically extract liquid from the liquid extraction box by rotating the turntable, and the liquid extraction plate can automatically move back and forth, so that the liquid extraction plate can completely extract liquid from the cover glass and the slide, and complete the comprehensive cleaning of the cover glass and the slide, without the need for frequent replacement of the cover glass and the slide. In addition, after the cleaning is completed, the corresponding liquid extraction plate can be automatically flipped over to ensure the cleanliness of the entire equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 This is a schematic structural diagram of an automated cell detection device proposed by the present invention;

[0048] Figure 2 for Figure 1 A magnified schematic diagram of part A;

[0049] Figure 3 This is a side view schematic diagram of the structure of an automated cell detection device proposed by the present invention;

[0050] Figure 4 for Figure 3 An enlarged schematic diagram of part B;

[0051] Figure 5 A schematic diagram of a turntable and its connection structure of an automated cell detection device proposed in the present invention;

[0052] Figure 6 A schematic diagram of a U-shaped frame and its connection structure of an automated cell detection device proposed by the present invention;

[0053] Figure 7 for Figure 6 A magnified schematic diagram of part C;

[0054] Figure 8 A schematic diagram of a liquid extraction plate and its connection structure of an automated cell detection device proposed by the present invention;

[0055] Figure 9 This is a schematic cross-sectional view of the liquid extraction box of an automated cell detection device proposed in the present invention.

[0056] In the figure: 1. Base; 2. Turntable; 3. Slide; 4. Cover glass; 5. Bracket; 6. Storage box; 7. U-shaped tube; 8. Curved rack; 9. Extraction box; 10. Collection box; 11. U-shaped frame; 12. Electron microscope; 13. Liquid outlet pipe; 14. Liquid inlet pipe; 15. Extraction pipe; 16. Piston rod; 17. First movable rod; 18. First turntable; 19. Turntable; 20. First belt; 21. Second turntable; 22. Second movable rod; 23. Extraction plate; 24. Telescopic rod; 25. Vertical plate ; 26. Mounting rod; 27. First column; 28. Missing gear; 29. ​​Second column; 30. Torsion spring; 31. Small gear; 32. One-way bearing; 33. First pulley; 34. Third column; 35. Large gear; 36. First bevel gear; 37. Second bevel gear; 38. Limiting rod; 39. Reciprocating screw; 40. First gear; 41. Piston plate; 42. Second belt; 43. Second pulley; 44. Rotating shaft; 45. Second gear; 46. Straight rack; 47. Connecting block; 48. Support column. DETAILED DESCRIPTION

[0057] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the present invention is further described below in conjunction with specific implementation methods.

[0058] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0059] Please see the attached Figure 1 -Attached Figure 2, an automated cell detection device, comprising a base 1, further comprising: a turntable 2, a carrying component, a liquid extraction and cleaning component and a driving component, the turntable 2 is rotatably arranged above the base 1, and four rectangular grooves evenly distributed in a ring are opened on the top of the turntable 2, there are four carrying components, and the four carrying components are distributed in a ring on the turntable 2, and the carrying component is used to carry cells to be detected, the liquid extraction and cleaning component is arranged on the base 1, and the liquid extraction and cleaning component is used to clean the carrying component, and the liquid extraction and cleaning component comprises: a liquid extraction plate 23, a flipping mechanism and a liquid extraction mechanism, there are two liquid extraction plates 23, and the corresponding sides of the two liquid extraction plates 23 are each provided with a liquid extraction port, the flipping mechanism is used to flip the corresponding liquid extraction plate 23, the liquid extraction mechanism is used to enable the liquid extraction plate 23 to extract the cell fluid on the carrying component, the driving component is arranged on the top of the base 1, and the driving component is used to make the turntable 2, the carrying component and the liquid extraction and cleaning component work;

[0060] As described above, after the carrying component carries the cells, the liquid extraction and cleaning component is activated by the driving component to clean the surface of the carrying component.

[0061] Please see the attached Figure 1 -Attached Figure 3 In a preferred embodiment, a bracket 5 is vertically mounted on the top of the base 1, and an electron microscope 12 is mounted on the bottom of the horizontal side of the bracket 5; a storage box 6 is also vertically mounted on the top of the base 1, and a liquid pump connected to the inside of the storage box 6 is mounted on the top of the storage box 6, and a U-shaped tube 7 is mounted on the liquid pump; an arc-shaped rack 8 is slidably provided on the top of the turntable 2, and a mounting rod 26 is fixed on the top of the arc-shaped rack 8, and the top end of the mounting rod 26 is fixedly mounted on the top of the bracket 5;

[0062] Specifically, the cell fluid is placed inside the storage box 6 , and the liquid pump draws the cell fluid inside the storage box 6 onto the slide 3 through the U-shaped tube 7 .

[0063] Please see the attached Figure 1 -Attached Figure 9 In a preferred embodiment, the carrying assembly includes: a slide 3, a cover glass 4, a limiting rod 38, a reciprocating screw 39 and a first gear 40. The slide 3 is horizontally installed inside the rectangular groove, the cover glass 4 is horizontally arranged above the slide 3, and an outer frame is installed around the cover glass 4. There are two limiting rods 38, both of which are vertically fixed to the top of the turntable 2, and the surface of the limiting rod 38 is slidably arranged inside the outer frame of the cover glass 4. The reciprocating screw 39 is vertically rotatably installed on the top of the turntable 2, and the surface of the reciprocating screw 39 is connected to the inner frame of the cover glass 4 by a thread. The first gear 40 is fixedly sleeved on the surface of the reciprocating screw 39, and the first gear 40 is engaged with the arc rack 8;

[0064] Furthermore, the slide 3 is rotated ninety degrees so that it is placed under the electron microscope 12. At this time, the first gear 40 will contact the arc rack 8 as it rotates and drive the first gear 40 to rotate. The rotation of the first gear 40 drives the reciprocating screw 39 to rotate, and the rotation of the reciprocating screw 39 drives the cover glass 4 to move downward.

[0065] Please see the attached Figure 2 -Attached Figure 9 In a preferred embodiment, the driving assembly includes: a U-shaped frame 11, a missing gear 28, a second column 29 and a torsion spring 30. The U-shaped frame 11 is fixedly mounted on the top of the base 1, and the top of the horizontal side of the U-shaped frame 11 is vertically rotatably mounted with a first column 27 fixed to the center of the bottom of the turntable 2. A driving motor is vertically mounted on the top of the base 1, and the output shaft of the driving motor is mounted on the bottom end of the first column 27. The missing gear 28 is fixedly sleeved on the surface of the first column 27, and the convex teeth on the missing gear 28 are divided into four groups, and the four groups of convex teeth are evenly spaced. The second column 29 is vertically rotatably mounted on the U The top of the horizontal side of the U-shaped frame 11 is provided with a one-way bearing 32 sleeved on the surface of the second column 29. The outer ring of the one-way bearing 32 is fixedly sleeved with a small gear 31, and the small gear 31 is meshed with the missing gear 28. A torsion spring 30 is sleeved on the surface of the second column 29, and the two ends of the torsion spring 30 are respectively fixedly mounted on the bottom of the small gear 31 and the top of the horizontal side of the U-shaped frame 11. A third column 34 is also vertically rotatably mounted on the top of the horizontal side of the U-shaped frame 11, and a large gear 35 is fixedly sleeved on the surface of the third column 34, and the large gear 35 is meshed with the small gear 31. A first bevel gear 36 is also fixedly sleeved on the surface of the third column 34.

[0066] Among them, a convex tooth on the missing gear 28 will first drive the small gear 31 to rotate one circle and cause the torsion spring 30 to generate torque. The rotation of the small gear 31 at this time will not drive the second column 29 to rotate through the one-way bearing 32. Since the large gear 35 and the small gear 31 are engaged, the rotation of the small gear 31 will drive the large gear 35 to rotate. The rotation of the large gear 35 will drive the first bevel gear 36 to rotate half a circle through the third column 34.

[0067] Please see the attached Figure 1 -Attached Figure 9In a preferred embodiment, the liquid extraction mechanism includes: a liquid extraction box 9, a second belt 42, a piston plate 41, a liquid inlet pipe 14, a liquid outlet pipe 13, a liquid extraction pipe 15 and a collection box 10. The liquid extraction box 9 is vertically installed on the top of the base 1, and a first turntable 18 is rotatably installed above the liquid extraction box 9. The second belt 42 is rotatably set on the side of the liquid extraction box 9, and a second pulley 43 is provided at both ends of the second belt 42. One of the second pulleys 43 is fixedly installed at the center of the end face of the first turntable 18 through a connecting shaft, and the other second pulley The end surface 43 is horizontally fixed with a rotating rod 19 that is rotatably mounted on the top of the base 1. The other end of the rotating rod 19 is fixed with a second bevel gear 37, and the first bevel gear 36 and the second bevel gear 37 are meshed. The piston plate 41 is slidably arranged inside the liquid pumping box 9, and the top of the piston plate 41 is vertically fixed with a piston rod 16. The top of the piston rod 16 passes through the liquid pumping box 9, and the top of the piston rod 16 rotates with a first movable rod 17 that is rotatably mounted on the outer edge of the side of the first turntable 18. The liquid inlet pipe 14 is connected and mounted on the top of the liquid pumping box 9, and the vertical side of the liquid inlet pipe 14 extends into Inside the liquid pumping box 9, the surface of the liquid inlet pipe 14 is slidably arranged inside the piston plate 41, a first one-way valve is installed on the liquid inlet pipe 14, the liquid outlet pipe 13 is connected and installed on the top of the liquid pumping box 9, and the vertical side of the liquid outlet pipe 13 extends into the interior of the liquid pumping box 9, the surface of the liquid outlet pipe 13 is slidably arranged inside the piston plate 41, a second one-way valve is installed on the liquid outlet pipe 13, the horizontal side of the liquid pumping pipe 15 is slidably installed inside the liquid inlet pipe 14 through a spline, and the liquid pumping pipe 15 is a three-way pipe, and the other two ends of the liquid pumping pipe 15 are respectively rotated and connected and installed on the end faces of the two liquid pumping plates 23. The collecting box 10 is vertically mounted on the top of the base 1, and the end of the liquid outlet pipe 13 is located inside the collecting box 10. A supporting column 48 is vertically rotatably mounted on the top of the base 1, and a second turntable 21 is rotatably mounted on the top of the supporting column 48. The first pulley 33 is fixedly sleeved on the surface of the supporting column 48 and the surface of the second column 29, and the first belt 20 is sleeved on the surface of the two first pulleys 33. A second movable rod 22 is rotatably mounted on the outer edge of the top of the second turntable 21, and the other end of the second movable rod 22 is rotatably mounted on the side of the liquid extraction plate 23 located above;

[0068] Preferably, when the first one-way valve on the liquid inlet tube 14 is opened, the cell fluid on the cover glass 4 and the slide 3 will be passed through the liquid extraction plate 23, the liquid extraction tube 15 and the liquid inlet tube 14 into the liquid extraction box 9 when the liquid extraction plate 23 passes through the cover glass 4 and the slide 3, thereby completing the cleaning of the cover glass 4 and the slide 3.

[0069] Please see the attached Figure 4 -Attached Figure 9In a preferred embodiment, the flip mechanism includes: a vertical plate 25 and a connecting block 47, the vertical plate 25 is vertically fixedly installed on the top of the base 1, and a telescopic rod 24 is horizontally fixed to the top of the side of the vertical plate 25, the fixed end and the telescopic end of the telescopic rod 24 are connected by a spline sliding connection, the connecting block 47 is fixedly installed on the top of the telescopic end of the telescopic rod 24, and two rotating shafts 44 are provided on the side of the connecting block 47, the rotating shaft 44 located above is fixedly installed between the connecting block 47 and the liquid extraction plate 23, and the two ends of the rotating shaft 44 located below rotate on the side of the connecting block 47 and are fixed to the end surface of the liquid extraction plate 23 respectively, and a second gear 45 is fixedly sleeved on the surface of the rotating shaft 44 located below, and a spur rack 46 is horizontally fixedly installed on the side of the vertical plate 25. When the spur rack 46 and the second gear 45 contact, the spur rack 46 and the second gear 45 engage;

[0070] Furthermore, after the cell fluid on the slide 3 is cleaned and returned, the second gear 45 contacts the spur rack 46 again, which resets the lower liquid extraction plate 23 to a state where the liquid extraction port faces upward, thereby ensuring the neatness of the equipment during detection.

[0071] The working principle of the present invention is as follows: the cell fluid is placed inside the storage box 6. When the cells need to be tested, the drive motor is started to drive the first column 27 to rotate 90 degrees. The rotation of the first column 27 drives the turntable 2 and the missing gear 28 to rotate 90 degrees. The rotation of the turntable 2 drives the cover glass 4, slide glass 3 and other connecting parts thereon to rotate 90 degrees. The cleaned cover glass 4 and slide glass 3 will be transferred to the loading station, and the liquid pump will extract the cell fluid inside the storage box 6 onto the slide glass 3.

[0072] The second operation of the driving motor will drive the glass slide 3 with the cell solution added to rotate ninety degrees so that it is placed under the electron microscope 12. At this time, the first gear 40 will follow the rotation and contact the arc-shaped rack 8 and drive the first gear 40 to rotate. The rotation of the first gear 40 drives the reciprocating screw 39 to rotate, and the rotation of the reciprocating screw 39 drives the cover glass 4 to move downward. It should be noted that when the glass slide 3 carrying the cell solution is located at the detection station, the cover glass 4 has already covered the cell solution, and then the cells are detected through the electron microscope 12;

[0073] The third drive motor drives the turntable 2 to rotate another 90 degrees, and the cells after detection will follow the slide 3 and the cover glass 4 to the stop position. It should be noted that during this process, when the first gear 40 and the arc-shaped rack 8 are engaged, the rotation of the first gear 40 will drive the cover glass 4 to move upward through the reciprocating screw 39, so as to separate the cover glass 4 from the slide 3.

[0074] Finally, the driving motor is started again, and the cover glass 4 and slide glass 3 on the stop station will be transferred to the cleaning station. During this period, a convex tooth on the missing gear 28 will first drive the small gear 31 to rotate one circle and make the torsion spring 30 generate torque. The rotation of the small gear 31 at this time will not drive the second column 29 to rotate through the one-way bearing 32. Since the large gear 35 and the small gear 31 are engaged, the rotation of the small gear 31 will drive the large gear 35 to rotate. The rotation of the large gear 35 will drive the first bevel gear 36 to rotate half a circle through the third column 34. The rotation of the first bevel gear 36 drives the second bevel gear 37 to rotate half a circle, and the third column 34 will rotate half a circle. The second bevel gear 37 drives the first turntable 18 to rotate half a circle through the rotating rod 19, the second belt 42 and the second pulley 43. The rotation of the first turntable 18 will drive the piston rod 16 to move downward through the first movable rod 17, and the piston rod 16 drives the piston plate 41 to move downward, so that the liquid inside the liquid extraction box 9 can be discharged to the inside of the collection box 10 through the liquid outlet pipe 13. When the missing gear 28 rotates ninety degrees, the cover glass 4 and the slide 3 will be located at the cleaning station, and the small gear 31 will no longer be engaged with the missing gear 28. The torsion force generated by the torsion spring 30 will cause the small gear 31 to rotate in the opposite direction for one circle, and further small gear 31 will rotate in the opposite direction for one circle. The wheel 31 will also cause the large gear 35 to rotate half a circle in the opposite direction to reset. The reverse rotation of the small gear 31 will drive the second column 29 to rotate through the one-way bearing 32. The rotation of the second column 29 drives the support column 48 to rotate through the first pulley 33 and the first belt 20. The rotation of the support column 48 drives the second turntable 21 to rotate one circle. The rotation of the second turntable 21 drives the second movable rod 22 to move. The movement of the second movable rod 22 drives the liquid extraction plate 23 to move back and forth once. During this period, the large gear 35 drives the first turntable 21 through the first bevel gear 36, the second bevel gear 37, the rotating rod 19, the second belt 42 and the second pulley 43 The disk 18 rotates. At this time, the rotation of the first turntable 18 drives the piston rod 16 and the piston plate 41 to move downward through the first movable rod 17. At this time, the second one-way valve on the liquid outlet pipe 13 is closed, and the first one-way valve on the liquid inlet pipe 14 is opened. Then, when the liquid extraction plate 23 passes through the cover glass 4 and the slide glass 3, the cell fluid on the cover glass 4 and the slide glass 3 will pass through the liquid extraction plate 23, the liquid extraction pipe 15 and the liquid inlet pipe 14 into the liquid extraction box 9, thereby completing the cleaning of the cover glass 4 and the slide glass 3, avoiding the need to frequently replace the cover glass 4 and the slide glass 3, and further ensuring the efficiency of cell detection;

[0075] When the liquid extraction plate 23 moves back and forth following the second movable rod 22, the straight rack 46 will engage with the second gear 45 and make the second gear 45 rotate half a circle. At the starting position, the lower liquid extraction plate 23 is in a state where the liquid extraction port is facing upward, thereby preventing the cell fluid adhered to the liquid extraction plate 23 from dripping. When the lower liquid extraction plate 23 moves upward toward the glass slide 3, the second gear 45 rotates half a circle, causing the liquid extraction port of the liquid extraction plate 23 to absorb the cell fluid downward. When the cell fluid on the glass slide 3 is cleaned and returned, the second gear 45 contacts the straight rack 46 again, which will reset the lower liquid extraction plate 23 to a state where the liquid extraction port is facing upward, thereby ensuring the neatness of the equipment during inspection.

[0076] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. An automated cell detection device, comprising a base (1), characterized in that: Also includes: A turntable (2), the turntable (2) being rotatably arranged above the base (1), and having four rectangular grooves evenly distributed in an annular shape on the top of the turntable (2); Carrying components, there are four carrying components, the four carrying components are distributed in a ring shape on the turntable (2), and the carrying components are used to carry cells to be detected; A liquid extraction and cleaning component, the liquid extraction and cleaning component is arranged on the base (1), and the liquid extraction and cleaning component is used to clean the bearing component; The liquid extraction and cleaning component comprises: There are two liquid extraction plates (23), and corresponding sides of the two liquid extraction plates (23) are each provided with a liquid extraction port; A flipping mechanism, the flipping mechanism being used to flip the corresponding liquid extraction plate (23); A liquid extraction mechanism, the liquid extraction mechanism is used to enable the liquid extraction plate (23) to extract the cell fluid on the carrier component; A drive assembly, the drive assembly being arranged on the top of the base (1), and being used to operate the turntable (2), the bearing assembly and the liquid extraction and cleaning assembly; The liquid pumping mechanism comprises: A liquid pumping box (9), the liquid pumping box (9) is vertically mounted on the top of the base (1), and a first turntable (18) is rotatably mounted above the liquid pumping box (9); A second belt (42), the second belt (42) is rotatably arranged on the side of the liquid extraction box (9), and second pulleys (43) are provided at both ends of the second belt (42); One of the second pulleys (43) is fixedly mounted at the center of the end face of the first turntable (18) via a connecting shaft, and a rotating rod (19) rotatably mounted on the top of the base (1) is horizontally fixed to the end face of the other second pulley (43), and a second bevel gear (37) is fixed to the other end of the rotating rod (19); The liquid pumping mechanism also includes: A piston plate (41), the piston plate (41) is slidably arranged inside the liquid pumping box (9), and a piston rod (16) is vertically fixed to the top of the piston plate (41), the top end of the piston rod (16) passes through the liquid pumping box (9), and the top end of the piston rod (16) is rotatably mounted on the outer edge of the side of the first rotating disk (18); A liquid inlet pipe (14), the liquid inlet pipe (14) is connected and installed on the top of the liquid pumping box (9), and the vertical side of the liquid inlet pipe (14) extends into the interior of the liquid pumping box (9), the surface of the liquid inlet pipe (14) is slidably arranged inside the piston plate (41), and a first one-way valve is installed on the liquid inlet pipe (14); a liquid outlet pipe (13), the liquid outlet pipe (13) being connected and installed at the top of the liquid pumping box (9), and the vertical side of the liquid outlet pipe (13) extending into the interior of the liquid pumping box (9), the surface of the liquid outlet pipe (13) being slidably arranged inside the piston plate (41), and a second one-way valve being installed on the liquid outlet pipe (13); A liquid extraction pipe (15), wherein the horizontal side of the liquid extraction pipe (15) is slidably mounted inside the liquid inlet pipe (14) via a spline, and the liquid extraction pipe (15) is a three-way pipe, and the other two ends of the liquid extraction pipe (15) are respectively rotatably connected and mounted on the end surfaces of the two liquid extraction plates (23); The collecting box (10) is vertically mounted on the top of the base (1), and the end of the liquid outlet pipe (13) is located inside the collecting box (10).

2. An automated cell detection device according to claim 1, characterized in that: A bracket (5) is vertically mounted on the top of the base (1), and an electron microscope (12) is mounted on the bottom of the horizontal side of the bracket (5); A storage box (6) is also vertically mounted on the top of the base (1), a liquid pump connected to the interior of the storage box (6) is mounted on the top of the storage box (6), and a U-shaped pipe (7) is mounted on the liquid pump; An arc-shaped rack (8) is slidably provided on the top of the turntable (2), and a mounting rod (26) is fixed on the top of the arc-shaped rack (8), and the top end of the mounting rod (26) is fixedly mounted on the top of the bracket (5).

3. An automated cell detection device according to claim 2, characterized in that: The bearing assembly includes: A glass slide (3), the glass slide (3) being horizontally mounted inside the rectangular groove; A cover glass (4), wherein the cover glass (4) is horizontally arranged above the slide glass (3), and an outer frame is installed around the cover glass (4); There are two limit rods (38), both of which are vertically fixed to the top of the turntable (2), and the surfaces of the limit rods (38) are slidably arranged inside the outer frame of the cover glass (4); A reciprocating screw (39), the reciprocating screw (39) is vertically rotatably mounted on the top of the turntable (2), and the surface of the reciprocating screw (39) is connected to the inside of the outer frame of the cover glass (4) through a thread; A first gear (40) is fixedly sleeved on the surface of the reciprocating screw (39), and the first gear (40) is meshed with the arc-shaped rack (8).

4. An automated cell detection device according to claim 3, characterized in that: The drive assembly includes: A U-shaped frame (11) is fixedly mounted on the top of the base (1), and a first column (27) fixed to the center of the bottom of the turntable (2) is vertically rotatably mounted on the top of the horizontal side of the U-shaped frame (11), a driving motor is vertically mounted on the top of the base (1), and an output shaft of the driving motor is mounted on the bottom end of the first column (27); A missing gear (28), wherein the missing gear (28) is fixedly sleeved on the surface of the first column (27), and the convex teeth on the missing gear (28) are divided into four groups, and the four groups of convex teeth are distributed at equal intervals; A second column (29), the second column (29) is vertically rotatably mounted on the top of the horizontal side of the U-shaped frame (11), and a one-way bearing (32) is sleeved on the surface of the second column (29), and a pinion (31) is fixedly sleeved on the outer ring of the one-way bearing (32), and the pinion (31) is meshed with the missing gear (28); A torsion spring (30) is sleeved on the surface of the second column (29), and two ends of the torsion spring (30) are respectively fixedly mounted on the bottom of the pinion (31) and the top of the horizontal side of the U-shaped frame (11).

5. An automated cell detection device according to claim 4, characterized in that: A third column (34) is also vertically rotatably mounted on the top of the horizontal side of the U-shaped frame (11), and a large gear (35) is fixedly sleeved on the surface of the third column (34), and the large gear (35) and the small gear (31) are meshed. A first bevel gear (36) is also fixedly sleeved on the surface of the third column (34), and the first bevel gear (36) and the second bevel gear (37) are meshed.

6. An automated cell detection device according to claim 5, characterized in that: A support column (48) is vertically rotatably mounted on the top of the base (1), and a second turntable (21) is rotatably mounted on the top of the support column (48); a first pulley (33) is fixedly sleeved on the surface of the support column (48) and the surface of the second column (29), and a first belt (20) is sleeved on the surfaces of the two first pulleys (33); A second movable rod (22) is rotatably mounted on the outer edge of the top of the second rotating disk (21), and the other end of the second movable rod (22) is rotatably mounted on the side of the liquid extraction plate (23) located above.

7. An automated cell detection device according to claim 6, characterized in that: The turning mechanism comprises: A vertical plate (25), the vertical plate (25) is vertically fixedly mounted on the top of the base (1), and a telescopic rod (24) is horizontally fixed to the top of the side of the vertical plate (25), and the fixed end and the telescopic end of the telescopic rod (24) are slidably connected via a spline; A connecting block (47) is fixedly mounted on the top of the telescopic end of the telescopic rod (24), and two rotating shafts (44) are provided on the side of the connecting block (47), the rotating shaft (44) located on the top is fixedly mounted between the connecting block (47) and the liquid extraction plate (23), and the two ends of the rotating shaft (44) located on the bottom rotate on the side of the connecting block (47) and are fixed on the end surface of the liquid extraction plate (23), respectively.

8. An automated cell detection device according to claim 7, characterized in that: A second gear (45) is fixedly sleeved on the surface of the rotating shaft (44) located below, and a spur rack (46) is fixedly installed horizontally on the side of the vertical plate (25). When the spur rack (46) and the second gear (45) are in contact, the spur rack (46) and the second gear (45) are meshed.

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