Traditional Chinese medicine compound preparation for preventing and treating retained fetal membranes of dairy cow

Abstract

The invention discloses a traditional Chinese medicine compound preparation for preventing and treating retained fetal membranes of dairy cows. The compound preparation is prepared from the following components in parts by weight: 45-55 parts of brown sugar, 10-20 parts of astragalus membranaceus, 15-25 parts of folium artemisiae argyi and 20-30 parts of motherwort. According to the formula, firstly, brown sugar and astragalus membranaceus are used for invigorating the stomach, strengthening the body resistance, improving the spleen and stomach functions of dairy cows and enhancing the qi and blood biochemical capacity of the body, so that the effect of recovering postpartum qi and blood weakness is achieved, Chinese angelica, ligusticum wallichii, prepared rehmannia root and other medicines with thick and greasy taste are not used for activating blood and enriching blood, on one hand, deficiency is not tonified, on the other hand, extravasated blood is not removed, and new blood is not generated; therefore, according to the formula, after the folium artemisiae argyi is used for warming uterus and dissipating extravasated blood, the motherwort is used for promoting blood circulation and removing blood stasis, dredging is used for tonifying, meanwhile, the brown sugar and the astragalus membranaceus are used for strengthening the spleen and stomach functions, and qi and blood are recovered by utilizing the qi and blood biochemical ability of the body, so that the body runs normally, and the fundamental purpose of treating diseases and solving the root is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese veterinary medicine, and particularly to a traditional Chinese medicine compound preparation for preventing and treating retention of fetal membranes in dairy cows, a preparation method thereof, and an application thereof. Background Art

[0002] The information provided in this part is only background information related to the present disclosure, and it is not necessarily prior art.

[0003] Retention of fetal membranes is one of the common postpartum diseases in dairy cows, which refers to a reproductive disorder disease in which the fetal membranes fail to fall off spontaneously within a certain period after parturition (12h - 24h) and remain in the uterus. The clinical characteristics are that the fetal membranes hang outside the vulva, initially bright red, and as time goes by, the color deepens to grayish white, and the vagina will discharge a foul-smelling, dirty red and turbid liquid. In large-scale dairy farms in China, the average incidence of retention of fetal membranes is about 10 - 20%. Retention of fetal membranes brings great harm to the cattle farm. The retained fetal membranes decompose and become putrid, and secondary infections can cause metritis and septicemia, which in turn affect milk production, and even threaten the life of dairy cows, and also lead to a decline in the reproductive ability of dairy cows.

[0004] Currently, antibiotics, oxytocin, choline drugs, and traditional Chinese veterinary medicines are often used to control retention of fetal membranes in dairy cows. However, due to the normal lactation of dairy cows after parturition, there is a risk of food residues in antibiotics, oxytocin, and choline drugs. Therefore, their continuous use in the treatment of dairy cows after parturition is restricted. Yimu Shenghua Powder is one of the few traditional Chinese veterinary medicine prescriptions for treating retention of fetal membranes on the market, but it has disadvantages such as poor curative effect, more times of oral administration, long course of treatment, and heavy workload. Moreover, for patents such as Chinese invention applications CN116115727A, CN110960610A, CN107669934A, CN105561173A, CN104800773A, etc., the formula is complex, the number of medicinal flavors used is large, the number of medication times is large, the course of treatment is long, and the effect of rapid cure cannot be achieved. Therefore, the present invention has developed a new traditional Chinese medicine compound in order to prevent and control retention of fetal membranes in dairy cows faster and more efficiently. Summary of the Invention

[0005] Object of the Invention: The technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition, a traditional Chinese medicine compound preparation, a product, and an application thereof for preventing and treating retention of fetal membranes in dairy cows in view of the deficiencies of the prior art.

[0006] Inventive concept: Cows are like human mothers and will experience physiological problems such as pregnancy, childbirth, and lactation. However, there is no concept of "sitting the month" for cows after giving birth like there is for women giving birth. Therefore, cows that are not adequately protected are at their most dangerous, have the highest incidence of diseases, and the highest mortality and culling rate during the 21-day period after giving birth. Traditional Chinese medicine states that "a woman after childbirth has a body as thin as paper," and the same is true for cows. After giving birth, they need to be carefully cared for to prevent pathogenic factors from invading the body due to weakness of qi and blood after childbirth. Currently, most cases of retained placenta in cows during childbirth are due to improper postpartum protection measures, exposure to wind and cold during childbirth, and the stagnation of cold causing retained placenta. Another main reason is that cows consume qi and disperse blood during childbirth, are extremely exhausted after childbirth, have a decreased uterine peristalsis, and are unable to expel the placenta. Therefore, through a dialectical analysis of the comprehensive pathogenesis of retained placenta, the main pathogenesis of retained placenta in cows is cold in the uterus and blood stasis, along with weakness of qi and blood. Therefore, the treatment principle is mainly to strengthen the healthy qi and dispel pathogenic factors, expel cold and remove blood stasis, and tonify both qi and blood. At the same time, cows have a decreased appetite after giving birth, often leading to negative energy balance and nutritional metabolic diseases. Therefore, after childbirth, it is also necessary to strengthen their spleen and stomach, enhance feed intake and the source of qi and blood generation, and reduce the occurrence of metabolic diseases. Traditional Chinese medicine has always prescribed medicine according to its "four natures and five flavors" to address the root causes of diseases. At the same time, traditional Chinese medicinal materials are increasingly popular around the world due to their advantages such as being inexpensive, green, safe, and residue-free. Therefore, this traditional Chinese medicine formula is developed as a protective cover for cows to "sit the month" after giving birth to resist pathogenic factors, strengthening the healthy qi on the one hand and dispelling pathogenic factors on the other to prevent and treat retained placenta in cows after childbirth.

[0007] To solve the above technical problems, the present invention discloses the following technical solutions:

[0008] In the first aspect, the present invention discloses a traditional Chinese medicine composition for preventing and / or treating retained placenta in cows, comprising the following components: brown sugar, astragalus membranaceus, artemisia argyi, and motherwort.

[0009] Brown sugar: It is warm in nature, sweet in taste, enters the spleen, and has the effects of replenishing qi and blood, strengthening the spleen and warming the stomach, alleviating middle-jiao pain, and dispelling cold and promoting blood circulation. It is commonly used for symptoms such as weakness of qi and blood after childbirth, disharmony between the spleen and stomach, and incomplete lochia after childbirth. Here, the property of brown sugar being sweet and entering the spleen is mainly utilized to strengthen the spleen and stomach function of cows after childbirth. The spleen and stomach are the foundation of acquired constitution and the source of qi and blood generation. Therefore, strengthening the spleen and stomach can restore their appetite after childbirth, increase feed intake, and quickly relieve postpartum fatigue. At the same time, brown sugar is red in color and enters the heart, and also has the function of nourishing blood. The reason for not using a large amount of tonic drugs such as angelica sinensis and rehmannia glutinosa is that for cows with retained placenta after childbirth, whether it is due to weakness of qi and blood or blood stasis, they are not receptive to medicine, as the deficiency cannot be tonified, and at the same time, without removing stasis, new blood cannot be generated. Using a large amount of tonic drugs will achieve only half the result with twice the effort. Therefore, for this syndrome, the aim is to activate blood circulation and remove blood stasis, while strengthening the spleen and stomach and supporting healthy qi, enabling the body's functions to naturally operate to generate qi and blood to nourish the whole body.

[0010] Astragalus membranaceus: It is warm in nature and sweet in taste, with the effects of greatly tonifying vital energy, strengthening the superficies, expelling pus, and promoting granulation for wound healing. It is used for symptoms such as insufficient vital energy after childbirth, qi deficiency and weakness, and birth canal injury. The main function of Astragalus membranaceus is to greatly tonify vital energy and strengthen the superficies. Traditional Chinese medicine holds that "a parturient woman's body is as thin as paper". Although a cow's hide is relatively thick, the birthing environment is poor, mostly in a leaky cowshed, so it is easy to be infected by exogenous pathogenic factors and cause diseases. Therefore, Astragalus membranaceus tonifies vital energy and strengthens the superficies. On the one hand, it adds a protective layer to the surface of the postpartum cow to resist the invasion of exogenous pathogenic factors into the body. Modern medicine explains it as enhancing the immune ability of the cow's body. On the other hand, it tonifies the qi deficiency after childbirth. Qi is the commander of blood, and blood is the mother of qi. When qi moves, blood moves. Therefore, Astragalus membranaceus is used in this prescription. In addition, due to postpartum midwifery, stillbirth, etc., cows often have birth canal injuries, which is one of the important causes of retained placenta. At the same time, Astragalus membranaceus is called "a holy medicine for expelling pus and promoting internal drainage of sores" in "Compendium of Materia Medica", and it has a good effect on promoting the healing of birth canal wounds.

[0011] Artemisia argyi: It is warm in nature, pungent and bitter in taste, with the effects of dispelling cold and relieving pain, warming the meridians and stopping bleeding, etc. It is mainly used for symptoms such as cold pain in the lower abdomen, irregular menstruation due to cold, infertility due to cold in the uterus, and vaginal bleeding during pregnancy, etc. Artemisia argyi is bitter in taste, warm in nature, can warm the liver and tonify fire, especially can enter the blood chamber (uterus), and specifically warms the uterus. Cows are likely to be infected by wind-cold during childbirth because the birthing place is simple and leaky. Therefore, Artemisia argyi is used to remove cold qi in the uterus. At the same time, Artemisia argyi is a medicine for tonifying fire, and fire can transform water, so it also has a certain effect of promoting blood circulation and dredging the meridians.

[0012] Leonurus japonicus: It is slightly cold in nature, bitter and pungent in taste, with the effects of promoting blood circulation and regulating menstruation, removing stasis and generating new blood. It is an important medicine for after childbirth, mainly used for symptoms such as blood stasis after childbirth, lochia not stopping, and retained placenta, etc. If stasis is not removed, new blood will not be generated. Therefore, Leonurus japonicus is used as a medicine for promoting blood circulation and removing stasis in this prescription.

[0013] In some embodiments, the composition further comprises white liquor or Vaccaria segetalis.

[0014] White liquor: It is warm in nature, sweet, bitter and pungent in taste, with the functions of promoting blood circulation, resisting cold qi, and enhancing the efficacy of drugs. It is mainly used for symptoms such as wind-cold arthralgia pain, and spleen-stomach deficiency-cold, etc. In the chapters on pregnancy and after childbirth of women in "Synopsis of the Golden Chamber", there are often cases of taking drugs with liquor. For example, when a woman has symptoms such as vaginal bleeding and vaginal bleeding during pregnancy (signs of miscarriage), Jiaoaixitang is taken with liquor to nourish blood and prevent miscarriage. At the same time, Artemisia argyi is also used here. When a woman has colic in the abdomen during pregnancy, Danggui Shaoyao Powder is taken with liquor; when a woman is in the early stage of pregnancy, Danggui San is taken with liquor to prevent miscarriage; when a woman is in the late stage of pregnancy, Baizhu San is taken with liquor to nourish the fetus; and when a parturient woman has abdominal pain after childbirth and there is blood stasis in the abdomen, Xiayuxuetang is decocted with liquor for treatment. It can be seen that liquor is often used as an adjuvant drug before and after childbirth in women. It is speculated that liquor has the functions of being warm in nature, promoting blood circulation and dredging the meridians, and guiding the drug to directly reach the uterus to exert its efficacy. Therefore, white liquor is added to this prescription.

[0015] Vaccaria segetalis: It is of neutral nature and bitter taste, and has the effects of promoting blood circulation and dredging meridians, inducing lactation, reducing swelling and astringing sores. The famous Jinchuangyao for treating sword and knife wounds is the Vaccaria Powder in Synopsis of the Golden Chamber mainly composed of Vaccaria segetalis. It is recorded in Synopsis of the Golden Chamber that it can be taken for severe sores and also for postpartum women. It can be seen that Vaccaria Powder was also used in ancient times for postpartum care. It is speculated that its main functions are to remove postpartum blood stasis and induce lactation at the same time. Therefore, Vaccaria segetalis is used in this formula.

[0016] In the formula provided by the present invention, the monarch drug is brown sugar (invigorating the spleen and warming the stomach, replenishing qi and blood), the ministerial drugs are Astragalus membranaceus (tonifying qi and consolidating the exterior, strengthening the healthy qi and eliminating pathogenic factors), the adjuvant drug is Artemisia argyi (warming the meridians to stop bleeding, warming the uterus, warming and promoting blood circulation to remove stasis), Leonurus japonicus is the guiding drug (activating blood circulation to regulate menstruation, removing stasis to promote new growth), and white liquor is the drug guide (promoting blood circulation, dispelling cold, and enhancing the efficacy of drugs).

[0017] This formula is formulated based on the principles of invigorating the spleen and warming the stomach, tonifying qi and consolidating the exterior, warming the uterus, activating blood circulation and dredging meridians, and removing stasis to promote new growth, achieving the effects of promoting the recovery of qi and blood in cows after parturition, activating blood circulation to remove stasis, and expelling fetal membranes.

[0018] In some embodiments, the weight parts of each component are as follows: 45 - 55 parts of brown sugar, 10 - 20 parts of Astragalus membranaceus, 15 - 25 parts of Artemisia argyi, 20 - 30 parts of Leonurus japonicus; in some embodiments, it is 47 - 53 parts of brown sugar, 12 - 18 parts of Astragalus membranaceus, 17 - 23 parts of Artemisia argyi, 22 - 28 parts of Leonurus japonicus; in some embodiments, it is 49 - 51 parts of brown sugar, 14 - 16 parts of Astragalus membranaceus, 19 - 21 parts of Artemisia argyi, 24 - 26 parts of Leonurus japonicus; in some embodiments, it is 50 parts of brown sugar, 15 parts of Astragalus membranaceus, 20 parts of Artemisia argyi, 25 parts of Leonurus japonicus.

[0019] In some embodiments, the composition further includes white liquor, and the weight parts of the white liquor are 45 - 55 parts, such as 47 parts, 49 parts, 50 parts, 51 parts or 53 parts. In some embodiments, the alcohol content of the white liquor is 50 - 54 degrees, such as 52 degrees. In some embodiments, the white liquor is 52 - degree red sorghum liquor.

[0020] In some embodiments, the composition further includes Vaccaria segetalis, and the weight parts of the Vaccaria segetalis are 10 - 20 parts, such as 12 parts, 14 parts, 15 parts, 16 parts or 18 parts.

[0021] In some embodiments, the cattle are dairy cows.

[0022] In some embodiments, the composition can also be used to prevent the occurrence of metritis.

[0023] In a second aspect, the present invention discloses a traditional Chinese medicine compound preparation for preventing and / or treating retention of fetal membranes in cattle.

[0024] In some embodiments, the traditional Chinese medicine compound preparation is made from the traditional Chinese medicine composition described in the first aspect above.

[0025] In some embodiments, the preparation method includes pulverizing astragalus membranaceus, artemisia argyi, and motherwort, passing through an 80-100 mesh sieve, and mixing with brown sugar; dissolving the obtained mixture in warm water for taking; in some embodiments, the preparation method includes pulverizing astragalus membranaceus, artemisia argyi, motherwort, and semen vaccariae, passing through an 80-100 mesh sieve, and mixing with brown sugar; dissolving the obtained mixture in warm water for taking; in some embodiments, the preparation method includes pulverizing astragalus membranaceus, artemisia argyi, and motherwort, passing through an 80-100 mesh sieve, and mixing with brown sugar and white wine, and dissolving the obtained mixture in warm water for taking. In some embodiments, if brown sugar lumps are used, the brown sugar lumps need to be pulverized into brown sugar powder before use, and if it is brown sugar powder, it can be used directly.

[0026] In some embodiments, the dosage form of the traditional Chinese medicine compound preparation is an oral dosage form.

[0027] In some embodiments, the cattle are dairy cows.

[0028] In a third aspect, the present invention discloses a product for preventing and / or treating retention of fetal membranes in cattle.

[0029] In some embodiments, the product includes the traditional Chinese medicine composition described in the first aspect above and / or the traditional Chinese medicine compound preparation described in the second aspect above.

[0030] In some embodiments, the cattle are dairy cows.

[0031] In a fourth aspect, the present invention discloses the application of the traditional Chinese medicine composition described in the first aspect above and / or the traditional Chinese medicine compound preparation described in the second aspect above in the preparation of a product for preventing and / or treating retention of fetal membranes in cattle.

[0032] In some embodiments, the cattle are dairy cows.

[0033] In the above third and fourth aspects, the product includes feed additives and feed.

[0034] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0035] (1) The traditional Chinese medicine composition provided by the present invention has the effects of strengthening the spleen and warming the stomach, replenishing qi and consolidating the exterior, warming the uterus, promoting blood circulation and dredging meridians, removing stasis and generating new blood. In this formula, brown sugar is used first, and astragalus membranaceus strengthens the stomach and strengthens the healthy qi, improving the spleen and stomach function of dairy cows and enhancing the ability of the body to generate qi and blood, so as to achieve the effect of restoring qi and blood weakness after childbirth. In this formula, drugs such as angelica sinensis, ligusticum wallichii, and rehmannia glutinosa, which are thick and greasy in taste, are not used for promoting blood circulation and tonifying blood. On the one hand, the weak body cannot tolerate tonics, and on the other hand, if stasis is not removed, new blood will not be generated. Simply tonifying blood will achieve half the result with twice the effort. Therefore, in this formula, it is wonderful to use artemisia argyi to warm the uterus and resolve stasis, and motherwort to promote blood circulation and remove stasis, and then tonify by dredging. At the same time, brown sugar and astragalus membranaceus strengthen the spleen and stomach function, and utilize the body's own spleen and stomach to restore qi and blood, enabling the body to operate normally, so as to achieve the fundamental purpose of treating the disease by seeking the root cause.

[0036] (2) All the medicinal materials in this prescription are common edible and medicinal homologous materials commonly used in folk households. The medication is simple and convenient, but the prescription principle is precise. Therefore, this prescription is fast and effective in treating retention of the fetal membranes, has a wide range of applications, and low cost. Whether it is a large-scale ranch or ordinary free-range households or farmers, they can all mix and use it by themselves, increasing the scope of use of this prescription.

[0037] (3) After dialectical treatment, the medication in this prescription is concise and the dosage is relatively strong, which can achieve the therapeutic effect of "knowing the effect after one dose and being cured after two doses". It strives to improve the curative effect of this prescription, reduce the number of times of using the prescription, reduce the workload of ranch workers and farmers, and is convenient and fast.

[0038] (4) The cure rate of the traditional Chinese medicine compound can reach over 80% at one time, and the course of treatment is 1 / 3 of that of conventional drug treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] The following further specifically describes the present invention in conjunction with the drawings and specific embodiments, and the above and / or other advantages of the present invention will become clearer.

[0040] Figure 1 It is a typical case of treating severe retention of the fetal membranes by administering Formula 1 in Example 1 once. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0041] According to the following embodiments, the present invention can be better understood. However, those skilled in the art can easily understand that the content described in the embodiments is only used to illustrate the present invention, and should not and will not limit the present invention described in detail in the claims.

[0042] In the following embodiments, the experimental methods are all conventional methods unless otherwise specified; the reagents and materials can be obtained from commercial channels unless otherwise specified.

[0043] The white liquor described in the following embodiments is 52-degree red sorghum white liquor.

[0044] The traditional Chinese medicine compatibility of the present invention is mainly used to explore the prevention and treatment effects of retention of the fetal membranes caused by qi and blood weakness and qi and blood stasis after dairy cows give birth, determine the best ratio and dosage in clinical practice, and solve the problems of poor efficacy of conventional western medicines and traditional Chinese veterinary medicines for preventing and controlling retention of the fetal membranes on the market, multiple treatment times, long treatment courses, and increased workload of ranch employees. The traditional Chinese medicine compound prepared by this formula can effectively prevent and treat the problem of retention of the fetal membranes after dairy cows give birth, thereby reducing the risk of dairy cows suffering from metritis and improving the production performance and reproductive performance of dairy cows.

[0045] Example 1: Clinical Trial of Traditional Chinese Medicine Compound Preparation for Treating Retention of the Fetal Membranes in Dairy Cows

[0046] 1. Materials and Methods

[0047] 1.1 Experimental animals

[0048] The experimental animals in this study were newly parturient Holstein cows. The experiment was conducted in a large-scale ranch. The experimental cows were fed and managed daily according to the existing feeding methods in the ranch, with a total mixed ration, fed 3 times a day, allowed free access to food and water, and the ranch was equipped with independent lying areas and exercise fields for the cows.

[0049] 1.2 Experimental drugs

[0050] To verify the reliability of the traditional Chinese medicine compound, we screened the prescriptions of a total of 3 prescriptions, namely Prescription 1, Prescription 2, and Prescription 3 of the traditional Chinese medicine compound, and screened the dosage of Prescription 4 (0.5 times the dosage of Prescription 1).

[0051] Positive western medicine control group: Cefotaxime, Bisekeling.

[0052] Positive traditional Chinese medicine control group: Yimu Shenghua Powder group: Yimu Shenghua Powder.

[0053] Prescription 1 (500 g of brown sugar, 150 g of astragalus membranaceus, 250 g of motherwort, 200 g of mugwort leaf, 500 mL of white wine).

[0054] Prescription 2 (500 g of brown sugar, 150 g of astragalus membranaceus, 250 g of motherwort, 200 g of mugwort leaf).

[0055] Prescription 3 (500 g of brown sugar, 250 g of astragalus membranaceus, 200 g of mugwort leaf, 200 g of motherwort, 150 g of vaccaria segetalis).

[0056] Prescription 4 (250 g of brown sugar, 75 g of astragalus membranaceus, 125 g of motherwort, 100 g of mugwort leaf, 250 mL of white wine).

[0057] For Prescriptions 1 - 4, the above medicinal materials were weighed, screened, cleaned, dried, mixed, and pulverized to 100 meshes for standby. After the traditional Chinese medicinal materials in Prescriptions 1 and 4 were pulverized, they were mixed with white wine.

[0058] 1.3 Diagnosis and identification of retained placenta

[0059] After parturition, 12 hours after the new cows gave birth, cows with retained placenta or partial retention of the fetal membranes were diagnosed with retained placenta.

[0060] 1.4 Inclusion criteria

[0061] Diseased cows with retained placenta 12 hours after parturition were selected as experimental subjects.

[0062] 1.5 Exclusion criteria

[0063] Cows that were listless after parturition or suffered from other diseases and needed to be treated with other drugs, which had an impact on the experiment.

[0064] 1.6 Cure criteria

[0065] After administration, if the fetal membranes of dairy cows completely fall off within 24 hours, it is judged as cured. If the fetal membranes still do not come off 24 hours after 3 doses (3 days) of administration, it is judged as not cured.

[0066] 1.6 Test method

[0067] Select 66 newly calved Holstein cows and randomly divide them into a positive western medicine control group (n = 11), a positive traditional Chinese medicine control group (n = 11), Prescription 1 group (n = 11), Prescription 2 group (n = 11), Prescription 3 group (n = 11), and Prescription 4 group (n = 11). In the positive western medicine control group, each dairy cow is intramuscularly injected with 20 mL of ceftiofur + 20 mL of bisekeling per day; in the positive traditional Chinese medicine control group, Prescription

[0068] 1, Prescription 2, Prescription 3, and Prescription 4 are respectively dissolved in 8 L of warm water according to the above prescriptions, stirred evenly and administered by gavage, once a day for each dairy cow. Observe and count the number of administrations and the cure situation of dairy cows with retained fetal membranes in each group every day.

[0069] 2. Test results

[0070] The test results are shown in Table 1 and Figure 1 as follows: The treatment results of the positive western medicine group and the positive traditional Chinese medicine group are similar, but the total cure rates are both lower than those of Prescription 1, Prescription 2, Prescription 3, and Prescription 4 groups (81.82% VS 90.91%). Among Prescription 1, Prescription 2, Prescription 3, and Prescription 4, it can be seen that the cure rate of Prescription 1 after one gavage (81.82%) is much higher than that of Prescription 2 (54.55%), Prescription 3 (54.55%), and Prescription 4 (45.45%). And the total cure rate of Prescription 1 after one gavage treatment (81.82%) is equivalent to the total cure rate of the positive western medicine group and the positive traditional Chinese medicine group after three treatments (81.82%). At the same time, the incidence of postpartum metritis in dairy cows in Prescription 1 and Prescription 4 groups is the lowest. The above test results show that Prescription 1 is the prescription with the fewest gavage times and the best treatment effect for treating retained fetal membranes.

[0071] Table 1 Cure rate of traditional Chinese medicine compound in treating retained fetal membranes of dairy cows

[0072]

[0073]

[0074] Example 2: Clinical trial of traditional Chinese medicine compound preparation for preventing retained fetal membranes in dairy cows

[0075] 1. Materials and methods

[0076] 1.1. Test animals

[0077] The test animals in this study were newly calved Holstein cows after parturition. The experiment was carried out in a large-scale ranch. The test cows were fed and managed daily according to the existing feeding methods on the ranch, fed a total mixed ration, fed 3 times a day, with free access to food and water. The ranch was equipped with independent lying areas and exercise fields for cows.

[0078] 1.2, Test drugs

[0079] Positive control group: Oxytocin.

[0080] Formula 1 (500 g of brown sugar, 150 g of astragalus membranaceus, 250 g of motherwort, 200 g of mugwort leaf, 500 mL of white liquor).

[0081] Weigh the above medicinal materials according to the weight ratio, screen, wash, dry, mix, and pulverize them to 100 meshes for standby.

[0082] 1.3 Inclusion criteria

[0083] Cows with retained fetal membranes 12 hours after parturition and no abnormal diseases or severe bleeding after parturition

[0084] 1.4 Exclusion criteria

[0085] Cows that experienced major events during parturition, were in poor spirits, and required additional treatment

[0086] 1.5 Cure criteria

[0087] After a single prophylactic administration, if the fetal membranes of the cows completely fell off within 24 hours, it was judged as normal fetal membrane shedding; otherwise, it was judged as retained fetal membranes.

[0088] 1.6 Test methods

[0089] Select 50 newly calved Holstein cows, with a positive control group (n = 25) and a Formula 1 test group (n = 25). In the positive control group, each cow was intramuscularly injected with 20 mL of oxytocin within 1 hour after parturition. In the Formula 1 test group, Formula 1 was dissolved in 8 L of warm water, stirred evenly, and administered by gavage to the cows within 1 hour after parturition. Subsequently, the two groups of cows were transferred to the new calving pen for postpartum care under the same conditions, and the incidence of retained fetal membranes, metritis, and death and culling of the two groups of cows within one month after parturition were observed.

[0090] 2. Test results

[0091] The test results are shown in Table 2. Compared with the positive drug group, the probability of retained fetal membranes in the Formula 1 group was reduced by 3 times, and the incidence of metritis was also significantly reduced. In terms of the death and culling rate, there was no death and culling of cows in the Formula 1 group after parturition, which was much lower than 12.00% in the positive drug group. The above results indicate that the traditional Chinese medicine compound Formula 1 has an obvious effect in preventing retained fetal membranes in cows and can effectively reduce the death and culling rate of cows within one month after parturition.

[0092] Table 2 Effect of Traditional Chinese Medicine Compound on Preventing Retained Placenta

[0093]

[0094] In summary, the traditional Chinese medicine compound can significantly improve the cure rate of retained placenta in dairy cows after a single administration, effectively prevent the risk of retained placenta in dairy cows after parturition, and at the same time, significantly reduce the mortality and culling rate of dairy cows after parturition. This shows that the traditional Chinese medicine compound can significantly and effectively prevent and treat the occurrence of retained placenta in dairy cows after parturition, and can completely replace the use of chemical drugs such as antibiotics, solving the problems of poor product effects, long treatment courses, and abuse of antibiotics in current market products.

[0095] The above-described embodiments merely represent several implementation manners of the present invention, and the description thereof is relatively specific and detailed, but should not be construed as a limitation on the scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the appended claims.

Claims

1. A Chinese medicinal composition for preventing and / or treating retained placenta in cattle, characterized in that: The invention comprises the following components in parts by weight: 45-55 parts of brown sugar, 10-20 parts of astragalus, 15-25 parts of wormwood leaves and 20-30 parts of motherwort.

2. The Chinese medicine composition according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 47-53 parts of brown sugar, 12-18 parts of astragalus, 17-23 parts of wormwood leaves, and 22-28 parts of motherwort.

3. The Chinese medicine composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 49-51 parts of brown sugar, 14-16 parts of astragalus, 19-21 parts of wormwood, 24-26 parts of motherwort, and 49-51 parts of white wine.

4. The Chinese medicine composition according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 50 parts of brown sugar, 15 parts of astragalus, 20 parts of wormwood leaves and 25 parts of motherwort.

5. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The composition further comprises liquor or fenugreek, wherein the liquor is present in 45-55 parts by weight, and the fenugreek is present in 10-20 parts by weight.

6. The Chinese medicine composition according to any one of claims 1 to 4, characterized in that: The cattle are dairy cows.

7. A Chinese medicinal compound preparation for preventing and / or treating retained placenta in cattle, characterized in that: Made from the Chinese medicine composition described in any one of claims 1 to 6; the method of making the Chinese medicine composition into a Chinese medicine compound preparation comprises the steps of crushing astragalus, wormwood, and motherwort, passing through a 80-100 mesh sieve, and mixing with brown sugar; or crushing astragalus, wormwood, motherwort, and fenugreek, passing through a 80-100 mesh sieve, and mixing with brown sugar; or crushing astragalus, wormwood, and motherwort, passing through a 80-100 mesh sieve, and mixing with brown sugar and liquor.

8. The Chinese medicinal compound preparation according to claim 7, characterized in that: The dosage form of the Chinese medicine compound preparation is an oral dosage form; the cow is a dairy cow.

9. A product for preventing and / or treating retained placenta in cattle, characterized in that: It includes the Chinese medicine composition according to any one of claims 1 to 6 or the Chinese medicine compound preparation according to claim 7 or 8.

10. Use of the Chinese medicine composition according to any one of claims 1 to 6 or the Chinese medicine compound preparation according to claim 7 or 8 in the preparation of products for preventing and / or treating retained placenta in cattle.

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