Traditional Chinese medicine composition for treating beriberi as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for treating beriberi as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of wormwood, 10 to 15 parts of dandelion, 5 to 10 parts of herba artemisiae scopariae, 10 to 15 parts of pericarpium citri reticulatae, 5 to 10 parts of herba portulacae, 3 to 5 parts of radix sophorae flavescentis, 5 to 10 parts of felwort, 5 to 10 parts of radix et rhizoma rhei, 2 to 5 parts of puffball, 1 to 3 parts of herba violae and 1 to 3 parts of fructus forsythiae. The traditional Chinese medicine composition prepared by the invention has a remarkable inhibition effect on pathogenic bacteria Candida albicans of beriberi.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for treating athlete's foot, its preparation method and application. Background Art

[0002] Tinea pedis (athlete's foot) is a superficial fungal infection disease caused by dermatophytes such as Trichophyton rubrum, Trichophyton mentagrophytes, and Candida albicans. It mainly occurs in the foot area, such as between the toes and the sole, and the common manifestations are blisters, desquamation, and accompanied by a certain sense of itching. The common symptoms of athlete's foot include skin keratosis with scales, increased peeling, cracking; local skin blisters or erosion between the toes, exudation, accompanied by itching, etc. At present, clinical treatment mainly relies on topical medications, and commonly used drugs include miconazole nitrate cream, ketoconazole cream, bifonazole solution, etc. However, when the area of tinea pedis is too large, the effect is often poor, the treatment time is long, the patient compliance is poor, and the patient stops taking the medicine before being completely cured, which will result in repeated attacks and drug resistance. Therefore, it is necessary to provide a traditional Chinese medicine composition with strong bactericidal effect and good treatment effect on large-area tinea pedis. Summary of the Invention

[0003] The purpose of the present invention is to provide a traditional Chinese medicine composition for treating athlete's foot, which has a significant killing effect on Candida albicans and can effectively treat athlete's foot caused by Candida albicans.

[0004] In order to achieve the above-mentioned invention purpose, the present invention provides the following technical solutions:

[0005] The present invention provides a traditional Chinese medicine composition for treating athlete's foot, and the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 10-15 parts of Artemisia argyi, 10-15 parts of Taraxacum mongolicum, 5-10 parts of Artemisia capillaris, 10-15 parts of Citrus reticulata Blanco, 5-10 parts of Portulaca oleracea, 3-5 parts of Sophora flavescens, 5-10 parts of Gentiana scabra, 5-10 parts of Rheum palmatum, 2-5 parts of Lasiosphaera fenzlii, 1-3 parts of Viola philippica, 1-3 parts of Forsythia suspensa.

[0006] The present invention also provides a preparation method of the traditional Chinese medicine composition for treating athlete's foot according to claim 1, comprising the following steps:

[0007] (1) Take Artemisia argyi, Taraxacum mongolicum, Artemisia capillaris, and Citrus reticulata Blanco, pulverize them to obtain traditional Chinese medicine powder A, and set aside;

[0008] (2) Take Portulaca oleracea, Sophora flavescens, Gentiana scabra, Rheum palmatum, Lasiosphaera fenzlii, Viola philippica, and Forsythia suspensa, decoct them with water, and filter to obtain a first filtrate and a first filter residue;

[0009] (3) Add water to the first filter residue and decoct it again, and filter to obtain a second filtrate;

[0010] (4) Combine the primary filtrate and the secondary filtrate, and obtain Chinese medicine powder B after concentration, drying, and pulverization.

[0011] (5) Mix the Chinese medicine powder A and the Chinese medicine powder B to obtain a Chinese medicine composition for treating athlete's foot.

[0012] Preferably, the pulverization particle size of the Chinese medicine powder A in step (1) is 40 - 60 mesh.

[0013] Preferably, the dosage of water in step (2) is 2 - 3 times the total mass of purslane, sophora flavescens, gentiana scabra, rhubarb, lasiosphaera fenzlii, viola yedoensis, and forsythia suspensa.

[0014] Preferably, the decocting temperature in step (2) is 90 - 110 °C, and the decocting time is 20 - 40 min.

[0015] Preferably, the water addition amount in step (3) is 1 - 2 times the mass of the primary filter residue.

[0016] Preferably, the decocting temperature in step (3) is 80 - 90 °C, and the decocting time is 40 - 60 min.

[0017] Preferably, the particle size of the Chinese medicine powder B in step (4) is 40 - 60 mesh.

[0018] The present invention also provides the application of the described Chinese medicine composition or the Chinese medicine composition prepared by the described preparation method in the preparation of a drug for treating athlete's foot.

[0019] Preferably, the drug further includes pharmaceutically common excipients; the dosage form of the drug is a foot bath agent.

[0020] The Chinese medicine composition provided by the present invention has a significant inhibitory effect on the athlete's foot pathogen candida albicans. The present invention combines a variety of Chinese medicines in compatibility. Artemisia capillaris, citrus reticulata, gentiana scabra, and forsythia suspensa play the role of clearing heat and removing dampness; purslane, rhubarb, and lasiosphaera fenzlii play the role of cooling blood and detoxifying; mugwort, dandelion, sophora flavescens, and viola yedoensis jointly play the role of sterilizing and relieving itching. The combination of multiple Chinese medicines interacts with each other and has a significant therapeutic effect on athlete's foot. Patients with skin erosion caused by athlete's foot can also use it, and moreover, it has a quick effect and does not relapse. Detailed Embodiments

[0021] The technical solutions provided by the present invention are described in detail below with reference to the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0022] Example 1

[0023] Take 10 parts of Artemisia argyi, 10 parts of Taraxacum mongolicum, 5 parts of Artemisia capillaris, and 10 parts of dried tangerine peel, crush them with a powder mill and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder A; take 5 parts of Portulaca oleracea, 3 parts of Sophora flavescens, 5 parts of Gentiana scabra, 5 parts of Rheum palmatum, 2 parts of Lasiosphaera fenzlii, 1 part of Viola philippica, and 1 part of Forsythia suspensa, place them in a frying pan, add 2 times the mass of water, and decoct at 100 °C for 30 minutes. Filter to separate the residue to obtain the first filtrate; take the residue, add 2 times the mass of water, and decoct at 90 °C for 50 minutes. After filtration, obtain the second filtrate. Combine the first filtrate and the second filtrate, concentrate to 1 / 5 of the original volume at 100 °C, dry at 65 °C, and then crush and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder B. Mix traditional Chinese medicine powder A and traditional Chinese medicine powder B to obtain the traditional Chinese medicine composition for treating athlete's foot.

[0024] Example 2

[0025] Take 12 parts of Artemisia argyi, 12 parts of Taraxacum mongolicum, 8 parts of Artemisia capillaris, and 12 parts of dried tangerine peel, crush them with a powder mill and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder A; take 8 parts of Portulaca oleracea, 4 parts of Sophora flavescens, 8 parts of Gentiana scabra, 8 parts of Rheum palmatum, 3 parts of Lasiosphaera fenzlii, 2 parts of Viola philippica, and 2 parts of Forsythia suspensa, place them in a frying pan, add 2 times the mass of water, and decoct at 100 °C for 30 minutes. Filter to separate the residue to obtain the first filtrate; take the residue, add 3 times the mass of water, and decoct at 90 °C for 50 minutes. After filtration, obtain the second filtrate. Combine the first filtrate and the second filtrate, concentrate to 1 / 5 of the original volume at 100 °C, dry at 65 °C, and then crush and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder B. Mix traditional Chinese medicine powder A and traditional Chinese medicine powder B to obtain the traditional Chinese medicine composition for treating athlete's foot.

[0026] Example 3

[0027] Take 15 parts of Artemisia argyi, 15 parts of Taraxacum mongolicum, 10 parts of Artemisia capillaris, and 15 parts of dried tangerine peel, crush them with a powder mill and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder A; take 10 parts of Portulaca oleracea, 5 parts of Sophora flavescens, 10 parts of Gentiana scabra, 10 parts of Rheum palmatum, 5 parts of Lasiosphaera fenzlii, 3 parts of Viola philippica, and 3 parts of Forsythia suspensa, place them in a frying pan, add 3 times the mass of water, and decoct at 100 °C for 30 minutes. Filter to separate the residue to obtain the first filtrate; take the residue, add 2 times the mass of water, and decoct at 90 °C for 50 minutes. After filtration, obtain the second filtrate. Combine the first filtrate and the second filtrate, concentrate to 1 / 5 of the original volume at 100 °C, dry at 65 °C, and then crush and sieve through a 50-mesh sieve to obtain traditional Chinese medicine powder B. Mix traditional Chinese medicine powder A and traditional Chinese medicine powder B to obtain the traditional Chinese medicine composition for treating athlete's foot.

[0028] Comparative Example 1

[0029] Compared with Example 3, remove the traditional Chinese medicine Lasiosphaera fenzlii and Artemisia argyi. The specific preparation method is as follows:

[0030] Take 15 parts of dandelion, 10 parts of artemisia capillaris, and 15 parts of dried tangerine peel, pulverize them with a powder mill and pass through a 50-mesh sieve to obtain traditional Chinese medicine powder A; take 10 parts of purslane, 5 parts of sophora flavescens, 10 parts of gentiana scabra, 10 parts of rhubarb, 3 parts of viola yedoensis, and 3 parts of forsythia suspensa, place them in a frying pan, add 3 times the mass of water, and decoct at 100 °C for 30 minutes. Filter to separate the residue to obtain the first filtrate; add 2 times the mass of water to the residue and decoct at 90 °C for 50 minutes, and filter to obtain the second filtrate. Combine the first filtrate and the second filtrate, concentrate to 1 / 5 of the original volume at 100 °C, dry at 65 °C, and then pulverize and pass through a 50-mesh sieve to obtain traditional Chinese medicine powder B. Mix traditional Chinese medicine powder A and traditional Chinese medicine powder B to obtain the traditional Chinese medicine composition for treating athlete's foot.

[0031] Experimental Example 1

[0032] Take the traditional Chinese medicine compositions of Examples 1 to 3 and Comparative Example 1, and dilute them with RPMI1640 culture medium respectively to obtain traditional Chinese medicine solutions with concentrations of 4 mg / ml, 2 mg / ml, 1 mg / ml, 0.5 mg / ml, 0.25 mg / ml, and 0.125 mg / ml respectively.

[0033] After activating Candida albicans, inoculate it into a sterilized SDB medium, and carry out purification culture at 37 °C. Then use RPMI1640 to adjust the bacterial concentration to 1×10 5 CFU / ml. When in use, dilute it with RPMI1640 to a bacterial suspension with a concentration of 1×10 3 CFU / ml.

[0034] Use a pipette to respectively aspirate 100 μl of the traditional Chinese medicine solutions at each concentration of Examples 1 to 3 and Comparative Example 1, and add them to wells 1 to 6 in the first row of their respective 96-well bacterial culture plates in sequence, with 5 parallels set in each well; then add 100 μl of the diluted Candida albicans bacterial suspension. Add 100 μl of the Candida albicans bacterial suspension and 100 μl of RPMI1640 culture medium to well 7 as a negative control, and add 200 μl of RPMI1640 culture medium to well 8 as a blank control. After culturing in a 37 °C constant temperature incubator for 48 h, judge the results and calculate the average MIC.

[0035] After the culture is completed, use an enzyme-linked immunosorbent assay (ELISA) reader to measure the absorbance A 570nm , calculate the antibacterial rate, and use the concentration of the traditional Chinese medicine solution with an antibacterial rate greater than or equal to 80% as the MIC.

[0036] Antibacterial rate = (A negative control - A experimental group) / (A negative control - A blank control) × 100%

[0037] The results are shown in Table 1 below.

[0038] Table 1 Average MIC of traditional Chinese medicine solutions in each group

[0039] Group Example 1 Example 2 Example 3 Comparative Example 1 MIC (mg / ml) 0.5 0.5 0.5 2

[0040] As can be seen from Table 2, the minimum inhibitory concentration of the traditional Chinese medicine composition prepared by the present invention is 0.5 mg / ml, which is significantly better than that of Comparative Example 1. It shows that the traditional Chinese medicine composition prepared in this application has a significant inhibitory effect on Candida albicans, and the addition of Artemisia argyi and Lasiosphaera fenzlii significantly improves the inhibitory effect on Candida albicans.

[0041] Experimental Example 2

[0042] 1. Case Background

[0043] There were a total of 300 patients, aged 12 to 65 years old, including 232 males and 68 females. The course of the disease ranged from 7 days to 3 months. Among them, 132 were first-episode patients, 76 had relapsed once, and 92 had relapsed multiple times. The clinical manifestations were blisters, erosion, itching, pain, etc. on the interdigital space, foot margin, and sole of the foot.

[0044] 2. Treatment Method

[0045] Every night before going to bed, 5 g of the traditional Chinese medicine composition prepared in Example 3 of the present invention is brewed with 5 liters of hot water at 50 - 60 °C. After the temperature is suitable, soak the feet for 15 - 20 minutes. One course of treatment is 3 days. Patients with skin erosion can also use it. After one soak, the itching and pain can be effectively relieved. After 2 courses of treatment, the discomfort symptoms caused by athlete's foot basically disappear. It can be completely cured after 2 - 3 courses of treatment. Patients with multiple relapses can appropriately extend 1 - 2 courses of treatment for consolidation to prevent recurrence.

[0046] 3. Efficacy Evaluation

[0047] Efficacy evaluation criteria: Cure: The symptoms completely disappear and there is no recurrence within 1 month; Effective: The symptoms are significantly relieved; Ineffective: The symptoms do not relieve after 2 - 3 courses of treatment.

[0048] 4. Results

[0049] Table 2 Treatment effect of the traditional Chinese medicine composition prepared in Example 3

[0050] Therapeutic effect Cured Effective Ineffective Effective rate Number of people 279 15 6 98％

[0051] Effective rate = (Number of cured patients + Number of effective patients) / Total number of patients × 100%

[0052] As can be seen from Table 2, the traditional Chinese medicine composition prepared by the present invention has a significant curative effect on athlete's foot, and the effective rate reaches 98%.

[0053] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A Chinese medicine composition for treating beriberi, characterized in that: The Chinese medicine composition comprises the following raw materials in parts by weight: 10-15 parts of wormwood, 10-15 parts of dandelions, 5-10 parts of capillaries, 10-15 parts of tangerine peel, 5-10 parts of purslane, 3-5 parts of sophora flavescens, 5-10 parts of gentian, 5-10 parts of rhubarb, 2-5 parts of puffballs, 1-3 parts of viola yedoensis, and 1-3 parts of forsythia.

2. A method for preparing a Chinese medicine composition for treating beriberi according to claim 1, characterized in that: The following steps are involved: (1) Grind mugwort, dandelion, capillaris, and dried orange peel to obtain Chinese medicine powder A for later use; (2) adding water to purslane, sophora flavescens, gentian, rhubarb, puffball, viola yedoensis and forsythia to boil, filtering to obtain a primary filtrate and a primary filter residue; (3) adding water to the primary filter residue and boiling it again, and filtering to obtain a secondary filtrate; (4) combining the primary filtrate and the secondary filtrate, concentrating, drying and pulverizing to obtain Chinese medicine powder B; (5) After mixing the Chinese medicine powder A with the Chinese medicine powder B, a Chinese medicine composition for treating athlete's foot is obtained.

3. The preparation method according to claim 1, characterized in that: The pulverized particle size of the Chinese medicine powder A in step (1) is 40-60 meshes.

4. The preparation method according to claim 1, characterized in that: The amount of water used in step (2) is 2 to 3 times the total mass of purslane, sophora flavescens, gentian, rhubarb, puffball, viola yedoensis and forsythia.

5. The preparation method according to claim 1, characterized in that: The decoction temperature in step (2) is 90-110° C., and the decoction time is 20-40 minutes.

6. The preparation method according to claim 1, characterized in that: The amount of water added in step (3) is 1 to 2 times the mass of the primary filter residue.

7. The preparation method according to claim 1, characterized in that: The decoction temperature in step (3) is 80-90° C., and the decoction time is 40-60 min.

8. The preparation method according to claim 1, characterized in that: The particle size of the Chinese medicine powder B in step (4) is 40-60 mesh.

9. Use of the Chinese medicine composition according to claim 1 or the Chinese medicine composition prepared by the preparation method according to any one of claims 2 to 7 in preparing a medicament for treating beriberi.

10. The use according to claim 8, characterized in that The medicine also includes auxiliary materials commonly used in pharmacy; the dosage form of the medicine is a foot bath.