High-purity protein separation and purification device

Abstract

The invention belongs to the technical field of separation and purification, and particularly relates to a high-purity protein separation and purification device which comprises a supporting frame, separation cylinders are installed on the left side and the right side of the upper portion of the supporting frame, filter cylinders are installed in the separation cylinders, and a driving assembly used for driving the filter cylinders to rotate is installed on the supporting frame and located on the back faces of the separation cylinders. The support frame is provided with a material supply assembly for supplying materials to the filter cartridges, blockage can be treated without shutdown, when one group of filter cartridges is seriously blocked, related electromagnetic valves can be closed, the interiors of the group of separation cartridges can be backwashed through the cleaning assembly, and the other group of separation cartridges can continue to undertake the separation and purification work of high-purity protein, so that the separation efficiency is improved, and the separation efficiency is improved. Blockage treatment is realized without shutdown, and the working efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of separation and purification, and specifically to a high-purity protein separation and purification device. Background Art

[0002] In the biological field, the demand for high-purity proteins is increasing day by day. High-purity proteins have a wide range of applications in industries such as scientific research, pharmaceuticals, and food. However, separating and purifying high-purity proteins from complex biological samples is a challenging task. For example, in the separation and purification of collagen peptides, when the existing device uses a filter screen for filtration, impurities are easily blocked in the filter screen, which is not only inconvenient to clean, but also affects the filtration efficiency and effect, and further affects the separation and purification efficiency and effect of proteins.

[0003] In the prior art, the collagen peptide raw material is put into the interior of the purification tank, and the filter screen is used to achieve the filtration function of internal impurities, thereby completing the purification process. However, due to the extremely high viscosity of the collagen peptide stock solution, the filtration efficiency is very low, the overall processing process is slow, and after being blocked, it needs to be cleaned by backwashing. However, when cleaning by backwashing, it is necessary to stop the machine for cleaning, and when stopping the machine for cleaning, the separation and purification efficiency of high-purity proteins is reduced. For this reason, we propose a high-purity protein separation and purification device. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides a high-purity protein separation and purification device, which solves the problems of filter blockage and the need for machine stop cleaning in the background art.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A high-purity protein separation and purification device, including a support frame. On the left and right sides above the support frame, separation cylinders are installed. Inside the separation cylinders, filter cylinders are installed. On the support frame and located on the back of the separation cylinders, a driving component for driving the filter cylinders to rotate is installed. On the support frame, a feeding component for feeding materials to the filter cylinders is installed. A communication pipe is fixedly arranged between the two separation cylinders. The end of the filter cylinder is fixedly connected with a connecting pipe. The connecting pipe rotates and extends outside the separation cylinder, and the connecting pipe is communicated with the communication pipe through a rotary joint. On the support frame, a cleaning component for cleaning the interior of the separation cylinder is installed. Inside the filter cylinder, an anti-blocking component is installed. On the support frame, a material tank and a slag discharge tank are installed. A first solenoid valve is installed on the communication pipe. A feeding pipe is fixedly communicated between the material tank and the corresponding separation cylinder. A second solenoid valve is installed on the feeding pipe. Two slag discharge pipes are fixedly communicated between the slag discharge tank and the communication pipe. A third solenoid valve is installed on the slag discharge pipe.

[0006] As a further aspect of the present invention, the feeding assembly includes a feed pump, which is fixedly installed on the support frame by bolts. The feed end of the feed pump is fixedly communicated with a feed pipe, and the discharge end of the feed pump is fixedly communicated with a main supply pipe. Two groups of branch pipes are fixedly communicated with the main supply pipe. A fourth solenoid valve is installed on the branch pipe, and the end of the branch pipe away from the main supply pipe is rotatably communicated with the filter cylinder.

[0007] As a further aspect of the present invention, the driving assembly includes a double-shaft motor, which is fixedly installed on the support frame by bolts. The output end of the double-shaft motor is fixedly connected with a driving rod, and the end of the driving rod is fixedly connected with a driving gear. A driven gear is fixedly sleeved on the outer surface of the connecting pipe, and the driven gear meshes with the driving gear.

[0008] As a further aspect of the present invention, the anti-blocking assembly includes a rotating rod, which rotatably penetrates through the branch pipe. A spiral blade is spirally sleeved on the outer surface of the rotating rod. A transmission rod is rotatably installed on the support frame. A first pulley is fixedly sleeved on the outer surface of the transmission rod. A second pulley is fixedly sleeved on the outer surface of the driving rod on the double-shaft motor. A first transmission belt is tensionedly sleeved between the second pulley and the first pulley. A third pulley is fixedly connected to the end of the transmission rod. A fourth pulley is fixedly connected to the end of the rotating rod. A second transmission belt is tensionedly sleeved between the fourth pulley and the third pulley.

[0009] As a further aspect of the present invention, the spiral blade fits against the inner wall of the filter cylinder.

[0010] As a further aspect of the present invention, the cleaning assembly includes a circulation pump and a liquid tank. The circulation pump and the liquid tank are both installed on the support frame. The liquid inlet end of the circulation pump is fixedly communicated with the inside of the liquid tank through a pipeline. The liquid outlet end of the circulation pump is fixedly communicated with two groups of cleaning pipes. The liquid outlet ends of the cleaning pipes are fixedly communicated with the inside of the separation cylinder.

[0011] As a further aspect of the present invention, a filter screen is installed inside the liquid tank.

[0012] As a further aspect of the present invention, two groups of return liquid pipes are fixedly communicated with the liquid tank. The ends of the return liquid pipes are fixedly communicated with the connecting pipe, and a fifth solenoid valve is fixedly installed on the return liquid pipe.

[0013] The present invention provides a high-purity protein separation and purification device. Compared with the prior art, it has the following beneficial effects: 1. The high-purity protein separation and purification device can handle blockages without shutting down the machine. When one group of filter cylinders is severely blocked, the relevant solenoid valves can be closed, and the inside of the separation cylinders of this group can be backflushed through the cleaning component, while the other group of separation cylinders can continue to undertake the separation and purification work of high-purity protein, realizing the blockage treatment without shutting down the machine and improving work efficiency.

[0014] 2. The high-purity protein separation and purification device has high-efficiency separation and purification. The driving component drives the two groups of filter cylinders to rotate simultaneously to generate centrifugal force, enabling the finished product to pass through the filter cylinders and enter the feed box, and the slag material to enter the slag discharge box through the slag discharge pipe, effectively realizing the separation and purification of high-purity protein.

[0015] 3. The high-purity protein separation and purification device has good anti-blocking effect. The spiral blade in the anti-blocking component fits the inner wall of the filter cylinder, and the rotation direction is opposite to that of the filter cylinder, which can scrape off the blocking material adhering to the inner wall of the filter cylinder, prevent blockage within a certain range, and at the same time push the slag material towards the communicating pipe direction to facilitate the slag material to enter the slag discharge box.

[0016] 4. The high-purity protein separation and purification device can recycle the cleaning liquid. The liquid box in the cleaning component is equipped with a filter screen, which can filter the mixture of the refluxed cleaning liquid and the slag material, and the filtered cleaning liquid flows back into the liquid box to realize the recycling of the cleaning liquid and save resources.

[0017] 5. The high-purity protein separation and purification device has strong structural linkage. Each component works together through reasonable mechanical connections and transmission methods. For example, the driving component drives the filter cylinder to rotate through gear meshing, and the anti-blocking component realizes the rotation of the rotating rod and the spiral blade through belt transmission, ensuring the stability and reliability of the device operation. Description of the Drawings

[0018] Figure 1 Schematic diagram of the main structure of the present invention Figure 1 ; Figure 2 Schematic diagram of the main structure of the present invention Figure 2 ; Figure 3 Schematic diagram of the main structure of the present invention Figure 3 ; Figure 4 Schematic diagram of the cross-sectional structure of the main body of the present invention Figure 1 ; Figure 5 Schematic diagram of the cross-sectional structure of the main body of the present invention Figure 2 ; Figure 6 Of the present invention Figure 3 Enlarged structural schematic diagram of the structure at A in

[0019] In the figure: 1, support frame; 2, liquid tank; 3, slag discharge tank; 4, material box; 5, driven gear; 6, cleaning pipe; 7, fourth solenoid valve; 8, material pump; 9, separation cylinder; 10, branch pipe; 11, feed pipe; 12, transmission rod; 13, third pulley; 14, second transmission belt; 15, fourth pulley; 16, filter screen; 17, slag discharge pipe; 18, third solenoid valve; 19, rotating rod; 20, spiral blade; 21, filter cylinder; 22, first solenoid valve; 23, connecting pipe; 24, first transmission belt; 25, first pulley; 26, total supply pipe; 27, circulation pump; 28, second solenoid valve; 29, material conveying pipe; 30, connecting pipe; 31, rotary joint; 32, return liquid pipe; 33, fifth solenoid valve; 35, driving gear; 36, second pulley; 37, double-shaft motor. Specific embodiments

[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] Please refer to Figures 1-6 , the present invention provides a technical solution: a high-purity protein separation and purification device, including a support frame 1. On the upper left and right sides of the support frame 1, separation cylinders 9 are installed. Inside the separation cylinder 9, a filter cylinder 21 is installed. On the support frame 1 and located behind the separation cylinder 9, a driving assembly for driving the filter cylinder 21 to rotate is installed. On the support frame 1, a feeding assembly for feeding the filter cylinder 21 is installed. A connecting pipe 23 is fixedly arranged between the two separation cylinders 9. The end of the filter cylinder 21 is fixedly connected to a connecting pipe 30. The connecting pipe 30 rotates and extends outside the separation cylinder 9, and the connecting pipe 30 is communicated with the connecting pipe 23 through a rotary joint 31. On the support frame 1, a cleaning assembly for cleaning the inside of the separation cylinder 9 is installed. Inside the filter cylinder 21, an anti-blocking assembly is installed. On the support frame 1, a material box 4 and a slag discharge box 3 are installed. A first solenoid valve 22 is installed on the connecting pipe 23. A material conveying pipe 29 is fixedly communicated between the material box 4 and the corresponding separation cylinder 9. A second solenoid valve 28 is installed on the material conveying pipe 29. Two slag discharge pipes 17 are fixedly communicated between the slag discharge box 3 and the connecting pipe 23. A third solenoid valve 18 is installed on the slag discharge pipe 17.

[0022] During use, open one set of the fourth solenoid valves 7, and the feeding assembly conveys the high-purity protein to be separated and purified into the separation cylinder 9. Open the first solenoid valve 22, and the connecting pipe 23 connects the two sets of filter cylinders 21. Then, the driving assembly drives the two sets of filter cylinders 21 to rotate simultaneously. The rotating filter cylinders 21 will generate centrifugal force. At this time, the finished product will pass through the filter cylinders 21 and enter the material box 4 through the material conveying pipe 29, and the slag will enter the slag discharge pipe 3 through the slag discharge pipe 17. The provided anti-blocking assembly can scrape the inner wall of the filter cylinder 21, thereby preventing the filter cylinder 21 from being blocked within a certain range. When one set of the filter cylinders 21 is severely blocked, the first solenoid valve 22, one set of the fourth solenoid valves 7, one set of the second solenoid valves 28, and one set of the third solenoid valves 18 can be closed. Then, the cleaning assembly is used to perform backwashing on the inside of one set of the separation cylinders 9, and the other set of separation cylinders 9 can continue to perform the separation and purification work on the high-purity protein, so that the blockage can be processed without stopping the machine.

[0023] The feeding assembly includes a feed pump 8. The feed pump 8 is fixedly installed on the support frame 1 by bolts. The feed end of the feed pump 8 is fixedly connected to a feed pipe 11, and the discharge end of the feed pump 8 is fixedly connected to a main supply pipe 26. Two branch pipes 10 are fixedly connected to the main supply pipe 26. The fourth solenoid valves 7 are installed on the branch pipes 10. The end of the branch pipe 10 away from the main supply pipe is rotationally connected to the filter cylinder 21.

[0024] When the feeding assembly is in use, start the feed pump 8. The external liquid material enters the feed pump 8 through the feed pipe 11, then enters the main supply pipe 26, and finally enters the separation cylinder 9 through the branch pipe 10.

[0025] The driving assembly includes a double-shaft motor 37. The double-shaft motor 37 is fixedly installed on the support frame 1 by bolts. The output end of the double-shaft motor 37 is fixedly connected to a driving rod, and the end of the driving rod is fixedly connected to a driving gear 35. A driven gear 5 is fixedly sleeved on the outer surface of the connecting pipe 30. The driven gear 5 meshes with the driving gear 35.

[0026] When the driving assembly is in use, start the double-shaft motor 37. The double-shaft motor 37 drives the driving gear 35 to rotate through the driving rod. Since the driven gear 5 meshes with the driving gear 35 and the filter cylinder 21 is rotatably installed in the separation cylinder 9, the double-shaft motor 37 can drive the two sets of filter cylinders 21 to rotate simultaneously.

[0027] The anti-blocking component includes a rotating rod 19 which rotatably penetrates through the branch pipe 10. A spiral blade 20 is spirally sleeved on the outer surface of the rotating rod 19. A transmission rod 12 is rotatably installed on the support frame 1. A first belt pulley 25 is fixedly sleeved on the outer surface of the transmission rod 12. A second belt pulley 36 is fixedly sleeved on the outer surface of the driving rod on the double-shaft motor 37. A first transmission belt 24 is tensionedly sleeved between the second belt pulley 36 and the first belt pulley 25. One end of the transmission rod 12 is fixedly connected with a third belt pulley 13. One end of the rotating rod 19 is fixedly connected with a fourth belt pulley 15. A second transmission belt 14 is tensionedly sleeved between the fourth belt pulley 15 and the third belt pulley 13. The spiral blade 20 is in contact with the inner wall of the filter cylinder 21.

[0028] Since the first transmission belt 24 is tensionedly sleeved between the second belt pulley 36 and the first belt pulley 25, and the second transmission belt 14 is tensionedly sleeved between the fourth belt pulley 15 and the third belt pulley 13, the rotating rod 19 is driven to rotate. When the rotating rod 19 rotates, it will drive the spiral blade 20 to rotate. The spiral blade 20 is in contact with the inner wall of the filter cylinder 21. The rotating spiral blade 20 can scrape off the blocking material adhering to the inner wall of the filter cylinder 21, thereby preventing blockage within a certain range. The rotating directions of the spiral blade 20 and the filter cylinder 21 are opposite. The spiral blade 20 will push the slag towards the communicating pipe 23, facilitating the slag to enter the slag discharge box 3.

[0029] The cleaning component includes a circulating pump 27 and a liquid tank 2. Both the circulating pump 27 and the liquid tank 2 are installed on the support frame 1. The liquid inlet end of the circulating pump 27 is fixedly communicated with the inside of the liquid tank 2 through a pipeline. The liquid outlet end of the circulating pump 27 is fixedly communicated with two groups of cleaning pipes 6. The liquid outlet ends of the cleaning pipes 6 are fixedly communicated with the inside of the separation cylinder 9. A filter screen 16 is installed inside the liquid tank 2. Two groups of liquid return pipes 32 are fixedly communicated with the liquid tank 2. The ends of the liquid return pipes 32 are fixedly communicated with the communicating pipe 23. A fifth solenoid valve 33 is fixedly installed on the liquid return pipe 32.

[0030] A branch solenoid valve is installed on the cleaning pipe 6. The circulating pump 27 can be started. The cleaning liquid in the liquid tank 2 will enter the corresponding separation cylinder 9 to be cleaned through the cleaning pipe 6. Then, the cleaning liquid and the slag will enter the liquid tank 2 through the liquid return pipe 32. The installed filter screen 16 can filter the refluxed liquid, and the filtered liquid will flow back into the liquid tank 2.

[0031] Working principle: When in use, open one group of the fourth solenoid valves 7, and the feeding assembly conveys the high-purity protein to be separated and purified into the separation cylinder 9. Open the first solenoid valve 22, and the connecting pipe 23 connects the two groups of filter cylinders 21. Then start the driving assembly and start the double-shaft motor 37. The double-shaft motor 37 drives the driving gear 35 to rotate through the driving rod. Since the driven gear 5 meshes with the driving gear 35 and the filter cylinder 21 is rotatably installed in the separation cylinder 9, the double-shaft motor 37 can drive the two groups of filter cylinders 21 to rotate simultaneously. The driving assembly will drive the two groups of filter cylinders 21 to rotate simultaneously. The rotating filter cylinder 21 will generate centrifugal force. At this time, the finished product will pass through the filter cylinder 21 and enter the material box 4 through the material conveying pipe 29, and the slag will enter the slag discharge pipe 3 through the slag discharge pipe 17. The provided anti-blocking assembly can scrape the inner wall of the filter cylinder 21, thereby preventing the filter cylinder 21 from being blocked within a certain range. When one group of filter cylinders 21 is severely blocked, the first solenoid valve 22, one group of the fourth solenoid valves 7, one group of the second solenoid valves 28, and one group of the third solenoid valves 18 can be closed, and then the corresponding separation cylinder 9 inside is backwashed through the cleaning assembly, and the other separation cylinder 9 can continue to undertake the work of separating and purifying the high-purity protein, so that the blockage can be processed without stopping the machine; Since the second pulley 36 and the first pulley 25 are tightly sleeved with the first transmission belt 24, and since the fourth pulley 15 and the third pulley 13 are tightly sleeved with the second transmission belt 14, the rotating rod 19 is driven to rotate. When the rotating rod 19 rotates, it will drive the spiral blade 20 to rotate. The spiral blade 20 is attached to the inner wall of the filter cylinder 21. The rotating spiral blade 20 can scrape off the blocking material adhering to the inner wall of the filter cylinder 21, thereby preventing blockage within a certain range. The rotating direction of the spiral blade 20 is opposite to that of the filter cylinder 21. The spiral blade 20 will push the slag towards the connecting pipe 23, facilitating the slag to enter the slag discharge box 3; A branch solenoid valve is installed on the cleaning pipe 6. The circulating pump 27 can be started, and the cleaning liquid in the liquid tank 2 will enter the corresponding separation cylinder 9 to be cleaned through the cleaning pipe 6. Then the cleaning liquid and the slag will enter the liquid tank 2 through the return liquid pipe 32. The provided filter screen 16 can filter the reflux liquid, and the filtered liquid will flow back to the liquid tank 2.

[0032] It should be noted that in this text, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device.

[0033] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A high-purity protein separation and purification device, comprising a support frame (1), characterized in that: Separation cylinders (9) are installed on both the upper and left sides of the support frame (1), and a filter cylinder (21) is installed inside the separation cylinder (9). A driving assembly for driving the filter cylinder (21) to rotate is installed on the support frame (1) and located on the back side of the separation cylinder (9). A feeding assembly for feeding material to the filter cylinder (21) is installed on the support frame (1). A connecting pipe (23) is fixedly arranged between the two groups of separation cylinders (9). A connecting pipe (30) is fixedly connected to the end of the filter cylinder (21). The connecting pipe (30) is rotatably extended out of the outside of the separation cylinder (9), and the connecting pipe (30) is connected to the connecting pipe (23) through a rotating joint (31). 3), a cleaning component for cleaning the interior of the separation cylinder (9) is installed on the support frame (1), an anti-blocking component is installed inside the filter cylinder (21), a material box (4) and a slag discharge box (3) are installed on the support frame (1), a first solenoid valve (22) is installed on the connecting pipe (23), a material delivery pipe (29) is fixedly connected between the material box (4) and the corresponding separation cylinder (9), a second solenoid valve (28) is installed on the material delivery pipe (29), two groups of slag discharge pipes (17) are fixedly connected between the slag discharge box (3) and the connecting pipe (23), and a third solenoid valve (18) is installed on the slag discharge pipe (17).

2. A high-purity protein separation and purification device according to claim 1, characterized in that: The material supply assembly comprises a material pump (8), the material pump (8) being fixedly mounted on a support frame (1) by means of bolts, the material feed end of the material pump (8) being fixedly connected to a material feed pipe (11), the material discharge end of the material pump (8) being fixedly connected to a main supply pipe (26), the main supply pipe (26) being fixedly connected to two groups of branch pipes (10), the branch pipes (10) being mounted with a fourth solenoid valve (7), and the ends of the branch pipes (10) away from the main supply pipe being rotatably connected to a filter cartridge (21).

3. A high-purity protein separation and purification device according to claim 2, characterized in that: The driving assembly comprises a dual-axis motor (37), the dual-axis motor (37) being fixedly mounted on the support frame (1) by means of bolts, the output end of the dual-axis motor (37) being fixedly connected to a driving rod, the end of the driving rod being fixedly connected to a driving gear (35), the outer surface of the connecting tube (30) being fixedly sleeved with a driven gear (5), the driven gear (5) being meshed with the driving gear (35).

4. A high-purity protein separation and purification device according to claim 3, characterized in that: The anti-blocking component comprises a rotating rod (19), the rotating rod (19) is rotatably mounted on the branch pipe (10), the outer surface of the rotating rod (19) is spirally sleeved with a spiral blade (20), a transmission rod (12) is rotatably mounted on the support frame (1), the outer surface of the transmission rod (12) is fixedly sleeved with a first belt pulley (25), the outer surface of the driving rod on the dual-axis motor (37) is fixedly sleeved with a second belt pulley (36), a first transmission belt (24) is tensionedly sleeved between the second belt pulley (36) and the first belt pulley (25), an end of the transmission rod (12) is fixedly connected to a third belt pulley (13), an end of the rotating rod (19) is fixedly connected to a fourth belt pulley (15), and a second transmission belt (14) is tensionedly sleeved between the fourth belt pulley (15) and the third belt pulley (13).

5. A high-purity protein separation and purification device according to claim 4, characterized in that: The spiral blade (20) is in contact with the inner wall of the filter cartridge (21).

6. A high-purity protein separation and purification device according to claim 5, characterized in that: The cleaning assembly comprises a circulation pump (27) and a liquid tank (2), wherein the circulation pump (27) and the liquid tank (2) are both mounted on a support frame (1), the liquid inlet end of the circulation pump (27) is fixedly connected to the interior of the liquid tank (2) via a pipeline, the liquid outlet end of the circulation pump (27) is fixedly connected to two groups of cleaning pipes (6), and the liquid outlet ends of the cleaning pipes (6) are fixedly connected to the interior of a separation cylinder (9).

7. A high-purity protein separation and purification device according to claim 6, characterized in that: A filter screen (16) is installed inside the liquid tank (2).

8. A high-purity protein separation and purification device according to claim 7, characterized in that: Two groups of liquid return pipes (32) are fixedly connected to the liquid tank (2), the ends of the liquid return pipes (32) are fixedly connected to the connecting pipe (23), and a fifth solenoid valve (33) is fixedly installed on the liquid return pipe (32).

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