Centrifugal device for stem cell exosome processing

Abstract

The invention discloses a centrifugal device for stem cell exosome processing. The centrifugal device comprises a first device box body, and the interior of the first device box body is slidably connected with a second device box body. According to the centrifugal device for stem cell exosome processing, an electric push rod drives a second device box body to move downwards, so that the second device box body is retracted into a first device box body, a second rotating shaft can drive a sliding rod to move downwards, a sleeve rotates, and when the sleeve rotates, a worm can be driven to rotate; a worm drives a first stirring shaft on a first rotating shaft to rotate through a worm gear, a through hole in the first stirring shaft rotates from a horizontal position to a vertical position, at the moment, a sliding block on a sliding rod enters a vertical strip-shaped structure part from a spiral structure part of a sliding groove, a sleeve does not rotate any more, and finally a second stirring rod on a second stirring shaft can penetrate through the through hole; the height and the size of the whole device are greatly reduced, and the whole device is more convenient to move.

Description

Technical Field

[0001] The present invention relates to the technical field of exosome centrifugation separation, and more specifically, to a centrifugation device for processing stem cell exosomes. Background Art

[0002] Exosomes refer to small membrane vesicles containing complex RNA and proteins. Currently, they specifically refer to discoid vesicles with a diameter of forty to one hundred nanometers. All cultured cell types can secrete exosomes, and exosomes naturally exist in body fluids, including blood, saliva, urine, cerebrospinal fluid, and milk. They mainly originate from the formation of multivesicular bodies by the invagination of lysosomal particles within cells. After the outer membrane of the multivesicular body fuses with the cell membrane, they are released into the extracellular matrix. Currently, the processing of stem cell exosomes generally uses differential centrifugation. Differential centrifugation mainly separates organelles of different sizes by gradually increasing the centrifugation speed. The initial centrifugation speed is relatively low, and cells and cell debris precipitate at the bottom of the tube. The supernatant is taken and centrifuged at a higher centrifugation speed, and the vesicles and cell debris contained in the supernatant are sedimented again.

[0003] The Chinese patent discloses a centrifugation device for processing stem cell exosomes with the patent number CN202111361510.8, including a base and a support cylinder. The lower side of the base is connected with support feet, and an arc-shaped groove is provided on the upper side of the base. A support component is connected to the upper side of the base. The support component includes a lower connecting cylinder and an upper connecting rod. A spring is connected between the upper connecting rod and the lower connecting cylinder. An arc-shaped rotating part is provided on the lower side of the support cylinder, and a device box body is connected to the upper side of the support cylinder. A support plate is connected to the inner side of the device box body. The support plate is connected with a centrifuge tube and a rotating motor. The output shaft of the rotating motor is connected with a driving gear and a driving pulley. The centrifuge tube is connected with a driven gear and a stirring shaft. The stirring shaft is connected with a driven pulley and a stirring component; the present invention solves the problems in the prior art that using differential centrifugation to process highly viscous biological samples requires a long centrifugation step, a high centrifugation speed, and a low purity of the obtained exosomes, and is applicable to the processing of stem cell exosomes, with beneficial effects in cases. However, there are still certain problems. Since the device box body of this centrifugation device is an integral structure, and the overall volume is relatively large and the height is relatively high, it increases the difficulty of moving the whole device. Therefore, we provide a centrifugation device for processing stem cell exosomes. Summary of the Invention

[0004] The purpose of the present invention is to provide a centrifugation device for processing stem cell exosomes to solve the problems raised in the above background art:

[0005] The device box bodies of some existing centrifugation devices are of an integral structure, and the overall volume is relatively large and the height is relatively high, thus increasing the difficulty of moving the whole device.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A centrifugal device for processing stem cell exosomes, comprising a first device box body, inside which a second device box body is slidably connected, a first centrifuge tube is rotatably connected inside the first device box body, a second centrifuge tube is slidably connected inside the first centrifuge tube, the second centrifuge tube is rotatably connected to the second device box body, a first rotating shaft is rotatably connected inside the first centrifuge tube, a second rotating shaft is slidably connected inside the first rotating shaft, the second rotating shaft is rotatably connected to the second centrifuge tube, a first stirring shaft is rotatably connected inside the first rotating shaft, a first stirring rod is fixedly connected to the outside of the first stirring shaft, a second stirring shaft is fixedly connected inside the second rotating shaft, a second stirring rod is fixedly connected to the outside of the second stirring shaft, a through hole is opened inside the first stirring shaft, the through hole is used in cooperation with the second stirring rod, and a transmission mechanism is arranged inside the first rotating shaft.

[0008] Preferably, the transmission mechanism includes a sleeve, the sleeve is rotatably connected to the first rotating shaft, a worm is sleeved outside the sleeve, the worm is fixedly connected to the sleeve, a worm gear is sleeved outside the first stirring shaft, the worm gear is fixedly connected to the first stirring shaft, and the worm is meshed with the worm gear.

[0009] Preferably, a sliding rod is slidably connected inside the sleeve, the sliding rod is fixedly connected to the second rotating shaft, a sliding groove is opened inside the sleeve, a slider is slidably connected inside the sliding groove, the slider is fixedly connected to the sliding rod, and the sliding groove is of a spiral structure.

[0010] Preferably, a limiting groove is opened on one side of the second centrifuge tube close to the first centrifuge tube, a limiting plate is slidably connected inside the limiting groove, the limiting plate is fixedly connected to the first centrifuge tube, there are four limiting grooves in total, and the four limiting grooves are symmetrically distributed.

[0011] Preferably, the cross-sections of the first rotating shaft and the second rotating shaft are both of square structures.

[0012] Preferably, an external toothed ring is sleeved outside the first centrifuge tube, the external toothed ring is fixedly connected to the first centrifuge tube, a first motor is fixedly connected to the bottom of the first device box body, an output shaft of the first motor vertically penetrates the first device box body and extends to the inside of the first device box body, the output shaft of the first motor is rotatably connected to the first device box body, and a gear is fixedly connected to the output end of the first motor, and the gear is meshed with the external toothed ring.

[0013] Preferably, a second motor is fixedly connected to the top of the second device box body. The output shaft of the second motor vertically penetrates the second device box body and extends into the interior of the second centrifugal cylinder. The output shaft of the second motor is rotationally connected to the second device box body, and the second rotating shaft is fixedly connected to the output end of the second motor.

[0014] Preferably, an electric push rod is fixedly connected to the interior of the first device box body. The second device box body is fixedly connected to the output end of the electric push rod. A control panel is fixedly connected to the exterior of the first device box body. The first motor, the second motor, and the electric push rod are all electrically connected to the control panel.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] The electric push rod drives the second device box body to move downward, so that the second device box body is received into the interior of the first device box body. The second rotating shaft drives the sliding rod to move downward. The sliding rod presses against the inner wall of the sliding groove through the slider. Since the sliding groove is a spiral structure, the sleeve will rotate at this time. When the sleeve rotates, it will drive the worm to rotate. The worm drives the first stirring shaft on the first rotating shaft through the worm gear. As the first device box body continues to move downward, the through hole on the first stirring shaft rotates from the horizontal position to the vertical position. At this time, the slider on the sliding rod enters from the spiral structure part of the sliding groove into the vertical strip structure part. At this time, the sleeve no longer continues to rotate. Finally, the second stirring rod on the second stirring shaft will pass through the through hole until the second device box body is completely received into the first device box body, greatly reducing the overall height and volume of the device and making the overall movement more convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a schematic structural diagram of the second device box body of the present invention before descending;

[0018] Figure 2 is a schematic structural diagram of the second device box body of the present invention after descending;

[0019] Figure 3 is a schematic cross-sectional view of the whole of the present invention;

[0020] Figure 4 is a schematic structural diagram of the second centrifugal cylinder of the present invention;

[0021] Figure 5 is a schematic structural diagram of the second rotating shaft of the present invention before descending;

[0022] Figure 6 is a schematic structural diagram of the second rotating shaft of the present invention after descending;

[0023] Figure 7 is a schematic cross-sectional view of the second rotating shaft of the present invention;

[0024] Figure 8 Structural schematic diagram of the slider of the present invention;

[0025] Figure 9 Structural schematic diagram of the chute of the present invention.

[0026] Description of the reference numerals in the figure: 1. First device box body; 2. Second device box body; 3. First centrifuge tube; 4. Second centrifuge tube; 5. First rotating shaft; 6. Second rotating shaft; 7. First stirring shaft; 8. First stirring rod; 9. Second stirring shaft; 10. Second stirring rod; 11. Through hole; 12. Transmission mechanism; 13. Sleeve; 14. Worm; 15. Worm gear; 16. Slide bar; 17. Chute; 18. Slider; 19. Limit groove; 20. Limit plate; 21. External gear ring; 22. First motor; 23. Gear; 24. Second motor; 25. Electric push rod; 26. Control panel. Specific embodiments

[0027] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0028] Please refer to Figures 1 to 9 , a centrifuge for processing stem cell exosomes, including a first device box body 1, a second device box body 2 is slidably connected inside the first device box body 1, and the second device box body 2 can move up and down inside the first device box body 1, thereby reducing the overall occupied volume. A first centrifuge tube 3 is rotatably connected inside the first device box body 1, a second centrifuge tube 4 is slidably connected inside the first centrifuge tube 3, the second centrifuge tube 4 is rotatably connected to the second device box body 2, a first rotating shaft 5 is rotatably connected inside the first centrifuge tube 3, a second rotating shaft 6 is slidably connected inside the first rotating shaft 5, the second rotating shaft 6 is rotatably connected to the second centrifuge tube 4, a first stirring shaft 7 is rotatably connected inside the first rotating shaft 5, a first stirring rod 8 is fixedly connected to the outside of the first stirring shaft 7, a second stirring shaft 9 is fixedly connected inside the second rotating shaft 6, a second stirring rod 10 is fixedly connected to the outside of the second stirring shaft 9, a through hole 11 is opened inside the first stirring shaft 7, and the through hole 11 is used in cooperation with the second stirring rod 10. A transmission mechanism 12 is arranged inside the first rotating shaft 5, and when the second rotating shaft 6 moves downward, the transmission mechanism 12 can drive the first stirring shaft 7 on the first rotating shaft 5 to rotate.

[0029] Further, the transmission mechanism 12 includes a sleeve 13. The sleeve 13 is rotatably connected to the first rotating shaft 5. A worm 14 is sleeved outside the sleeve 13, and the worm 14 is fixedly connected to the sleeve 13. A worm gear 15 is sleeved outside the first stirring shaft 7, and the worm gear 15 is fixedly connected to the first stirring shaft 7. The worm 14 is meshed with the worm gear 15. When the sleeve 13 rotates, it will drive the worm 14 to rotate, and the worm 14 drives the first stirring shaft 7 to rotate through the worm gear 15.

[0030] Further, a slide bar 16 is slidably connected inside the sleeve 13. The slide bar 16 is fixedly connected to the second rotating shaft 6. A chute 17 is opened inside the sleeve 13. A slider 18 is slidably connected inside the chute 17, and the slider 18 is fixedly connected to the slide bar 16. The chute 17 is a spiral structure. Due to the chute 17 being arranged in a spiral structure, when the slide bar 16 moves downward, it will squeeze the inner wall of the chute 17 through the slider 18, thereby driving the sleeve 13 to rotate. A small part at the lower part of the chute 17 is a vertical strip structure. When the slider 18 enters from the spiral structure part into the vertical strip structure part, the sleeve 13 stops rotating. At this time, the through hole 11 on the first stirring shaft 7 rotates from the horizontal position to the vertical position for the second stirring rod 10 to pass through.

[0031] Further, a limiting groove 19 is opened on one side of the inner part of the second centrifuge tube 4 close to the first centrifuge tube 3. A limiting plate 20 is slidably connected inside the limiting groove 19, and the limiting plate 20 is fixedly connected to the first centrifuge tube 3. There are four limiting grooves 19 in total, and the four limiting grooves 19 are symmetrically distributed. Through the limiting grooves 19 and the limiting plates 20, the second centrifuge tube 4 can drive the first centrifuge tube 3 to rotate.

[0032] Further, the cross-sections of the first rotating shaft 5 and the second rotating shaft 6 are both square structures. The second rotating shaft 6 can move up and down inside the first rotating shaft 5. Due to the cross-sections of the first rotating shaft 5 and the second rotating shaft 6 being square structures, the second rotating shaft 6 can drive the first rotating shaft 5 to rotate synchronously.

[0033] Further, an external gear ring 21 is sleeved outside the first centrifuge tube 3, and the external gear ring 21 is fixedly connected to the first centrifuge tube 3. The bottom of the first device box body 1 is fixedly connected with a first motor 22. The output shaft of the first motor 22 vertically penetrates the first device box body 1 and extends to the inside of the first device box body 1. The output shaft of the first motor 22 is rotatably connected to the first device box body 1. The output end of the first motor 22 is fixedly connected with a gear 23, and the gear 23 is meshed with the external gear ring 21. The first motor 22 directly drives the gear 23 to rotate, and the gear 23 drives the first centrifuge tube 3 and the second centrifuge tube 4 to rotate through the external gear ring 21, so as to perform centrifugation operation on the supernatant samples of the stem cell culture solution inside the first centrifuge tube 3 and the second centrifuge tube 4.

[0034] Furthermore, a second motor 24 is fixedly connected to the top of the second device box body 2. The output shaft of the second motor 24 vertically penetrates through the second device box body 2 and extends into the interior of the second centrifuge cylinder 4. The output shaft of the second motor 24 is rotationally connected to the second device box body 2. The second rotating shaft 6 is fixedly connected to the output end of the second motor 24. The second motor 24 directly drives the first rotating shaft 5 and the second rotating shaft 6 to rotate. The supernatant sample of the stem cell culture medium is stirred by the first stirring shaft 7, the first stirring rod 8, the second stirring shaft 9, and the second stirring rod 10, accelerating the centrifugal processing of the supernatant sample of the stem cell culture medium.

[0035] Furthermore, an electric push rod 25 is fixedly connected to the interior of the first device box body 1. Four electric push rods 25 are symmetrically arranged. The electric push rod 25 is used to drive the second device box body 2 to move up and down, thereby adjusting the position of the second device box body 2. The second device box body 2 is fixedly connected to the output end of the electric push rod 25. A control panel 26 is fixedly connected to the exterior of the first device box body 1. The first motor 22, the second motor 24, and the electric push rod 25 are all electrically connected to the control panel 26. Electrical equipment is powered by an external power supply. Each electrical equipment is controlled through the control panel 26. The above is the existing well-known technology and will not be elaborated too much.

[0036] Usage steps of the present invention: When this centrifuge device for stem cell exosome processing is in use, the electric push rod 25 inside the first device box body 1 is started through the control panel 26, allowing the electric push rod 25 to drive the second device box body 2 to move downward, such that the second device box body 2 is received into the first device box body 1. During this process, the second centrifuge cylinder 4 will also be received into the first centrifuge cylinder 3, and the second rotating shaft 6 will also be received into the first rotating shaft 5. Moreover, when the second rotating shaft 6 is received into the first rotating shaft 5, the second rotating shaft 6 will drive the slide rod 16 to move downward. The slide rod 16 presses against the inner wall of the chute 17 through the slider 18. Since the chute 17 is a spiral structure, at this time, the sleeve 13 will rotate. When the sleeve 13 rotates, it will drive the worm 14 to rotate. The worm 14 drives the first stirring shaft 7 on the first rotating shaft 5 to rotate through the worm gear 15. As the first device box body 1 continues to move downward, the through hole 11 on the first stirring shaft 7 rotates from the horizontal position to the vertical position. At this time, the slider 18 on the slide rod 16 enters from the spiral structure part of the chute 17 into the vertical strip structure part. At this time, the sleeve 13 no longer continues to rotate. Finally, the second stirring rod 10 on the second stirring shaft 9 will pass through the through hole 11 until the second device box body 2 is completely received into the first device box body 1, greatly reducing the overall height and volume of the device and making the overall movement more convenient.

[0037] The basic principles, main features and advantages of the present invention have been shown and described above. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the descriptions in the specification are only preferred examples of the present invention and are not used to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A centrifugal device for processing stem cell exosomes, comprising a first device housing (1), characterized in that: The first device housing (1) is slidably connected to the second device housing (2), the first device housing (1) is rotatably connected to the first centrifugal cylinder (3), the first centrifugal cylinder (3) is slidably connected to the second centrifugal cylinder (4), the second centrifugal cylinder (4) is rotatably connected to the second device housing (2), the first centrifugal cylinder (3) is rotatably connected to the first rotating shaft (5), the first rotating shaft (5) is slidably connected to the second rotating shaft (6), the second rotating shaft (6) is rotatably connected to the second centrifugal cylinder (4), the first rotating shaft (5) is rotatably connected to the first stirring shaft (7), the first stirring shaft (7) is fixedly connected to the outside of the first stirring shaft (8), the second rotating shaft (6) is fixedly connected to the inside of the second stirring shaft (9), the second stirring shaft (9) is fixedly connected to the outside of the second stirring shaft (9), the first stirring shaft (7) is provided with a through hole (11), the through hole (11) is used in conjunction with the second stirring rod (10), and the first rotating shaft (5) is provided with a transmission mechanism (12).

2. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The transmission mechanism (12) comprises a sleeve (13), the sleeve (13) is rotatably connected to the first rotating shaft (5), a worm (14) is sleeved on the outside of the sleeve (13), the worm (14) is fixedly connected to the sleeve (13), a worm wheel (15) is sleeved on the outside of the first stirring shaft (7), the worm wheel (15) is fixedly connected to the first stirring shaft (7), and the worm (14) is meshingly connected to the worm wheel (15).

3. A centrifugal device for processing stem cell exosomes according to claim 2, characterized in that: The sleeve (13) is slidably connected to a slide rod (16) inside, and the slide rod (16) is fixedly connected to the second rotating shaft (6). The sleeve (13) is provided with a slide groove (17) inside, and the slide groove (17) is slidably connected to a slider (18) inside, and the slider (18) is fixedly connected to the slide rod (16). The slide groove (17) is a spiral structure.

4. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: A limiting groove (19) is provided on a side of the interior of the second centrifugal cylinder (4) close to the first centrifugal cylinder (3); a limiting plate (20) is slidably connected to the interior of the limiting groove (19); the limiting plate (20) is fixedly connected to the first centrifugal cylinder (3); there are four limiting grooves (19) in total, and the four limiting grooves (19) are symmetrically distributed.

5. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The cross sections of the first rotating shaft (5) and the second rotating shaft (6) are both square structures.

6. A centrifugal device for processing stem cell exosomes according to claim 1, characterized in that: The first centrifugal cylinder (3) is provided with an outer gear ring (21) on the outside, and the outer gear ring (21) is fixedly connected to the first centrifugal cylinder (3). The bottom of the first device housing (1) is fixedly connected to a first motor (22), and the output shaft of the first motor (22) vertically penetrates the first device housing (1) and extends to the inside of the first device housing (1). The output shaft of the first motor (22) is rotatably connected to the first device housing (1), and the output end of the first motor (22) is fixedly connected to a gear (23), and the gear (23) is meshingly connected to the outer gear ring (21).

7. A centrifugal device for processing stem cell exosomes according to claim 6, characterized in that: A second motor (24) is fixedly connected to the top of the second device housing (2); an output shaft of the second motor (24) vertically penetrates the second device housing (2) and extends to the interior of the second centrifugal cylinder (4); the output shaft of the second motor (24) is rotationally connected to the second device housing (2); and the second rotating shaft (6) is fixedly connected to the output end of the second motor (24).

8. A centrifugal device for processing stem cell exosomes according to claim 7, characterized in that: An electric push rod (25) is fixedly connected to the interior of the first device housing (1), the second device housing (2) is fixedly connected to the output end of the electric push rod (25), the exterior of the first device housing (1) is fixedly connected to a control panel (26), and the first motor (22), the second motor (24) and the electric push rod (25) are all electrically connected to the control panel (26).

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