Microorganism separation and extraction device

Abstract

The invention relates to the technical field of microorganism culture, in particular to a microorganism separation and extraction device which comprises a supporting seat and a separation cylinder, a main shaft penetrates through the top of the separation cylinder, a net plate is arranged in the separation cylinder, a plurality of stirring blades are fixed outside the main shaft, and the separation cylinder is connected with the supporting seat through a translation mechanism. Wherein the separation cylinder is connected with a release mechanism, a plurality of placement grooves distributed at equal intervals are formed in the bottom of the supporting seat, culture media are placed in the placement grooves, the release mechanism is used for discharging samples in the separation cylinder into the culture media, a supporting plate is fixed to the side wall of the main shaft, a sliding rod penetrates through the supporting plate, and the sliding rod is in sliding connection with the supporting plate. The sliding rod is connected with an adjusting mechanism; the adjusting mechanism is used for adjusting the height of the sliding rod; according to the device, the pretreated microorganism samples do not need to be manually transferred into different culture media, and the separation culture efficiency of the microorganism samples is effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microorganism culture, and specifically to a microorganism separation and extraction device. Background Art

[0002] Microorganisms include a large group of organisms such as bacteria, viruses, fungi, and some small protists, microscopic algae, etc. They are tiny in size and closely related to humans. They cover a wide variety of beneficial and harmful types and are widely involved in many fields such as food, medicine, industry and agriculture, environmental protection, sports, etc. Some microorganisms can be seen with the naked eye, such as mushrooms, ganoderma lucidum, shiitake mushrooms belonging to fungi, and there are also microorganisms that are acellular organisms composed of only a few components such as nucleic acids and proteins.

[0003] When separating and extracting microorganisms, it is necessary to pretreat the microorganisms. The purpose is to remove large particle impurities, retain the microorganisms, and dilute the microorganisms, so as to reduce the concentration of microorganisms in the sample and facilitate the separation of single colonies and counting.

[0004] Currently, after pretreating the microorganisms, it is also necessary to transfer the pretreated microorganism samples into different culture media, and multiple steps are carried out separately, which wastes manpower and reduces the efficiency of microorganism separation and extraction. Summary of the Invention

[0005] The purpose of the present invention is to provide a microorganism separation and extraction device to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] A microorganism separation and extraction device includes a support base and a separation cylinder. The top of the separation cylinder is penetrated by a main shaft, and the main shaft is rotatably connected to the top of the separation cylinder. A mesh plate is arranged inside the separation cylinder, and the side wall of the mesh plate is slidably connected to the inner wall of the separation cylinder. The mesh plate is connected with a vibration mechanism for driving the mesh plate to vibrate up and down. A guide cylinder penetrates through the mesh plate, a fixed rod is fixed on the outer wall of the guide cylinder, and the other end of the fixed rod is fixed to the inner wall of the separation cylinder. The outer wall of the guide cylinder is slidably connected to the inner wall of the mesh plate. The main shaft penetrates through the guide cylinder and is rotatably connected to the guide cylinder. A plurality of stirring blades are fixed on the outside of the main shaft. The separation cylinder is connected to the support base through a translation mechanism for driving the separation cylinder to move horizontally. Among them, the separation cylinder is connected with a release mechanism. A plurality of equally spaced placement grooves are arranged at the bottom of the support base, and culture media are placed inside the placement grooves. The release mechanism is used to discharge the samples inside the separation cylinder into the culture media. A support plate is fixed on the side wall of the main shaft, a sliding rod penetrates through the support plate, and the sliding rod is slidably connected to the support plate. The sliding rod is connected with an adjustment mechanism for adjusting the height of the sliding rod, so that the sliding rod controls the operation of the vibration mechanism and the release mechanism.

[0008] Preferably, the rotating mechanism includes a gear fixed to the top of the main shaft, and a toothed plate is fixed between the inner walls of the support base, and the toothed plate meshes with the gear.

[0009] Preferably, the translation mechanism includes a moving block fixed to the bottom of the separation cylinder, a threaded rod penetrates through the moving block, and the threaded rod is threadedly connected to the moving block. The two ends of the threaded rod are respectively rotatably connected to the two inner walls of the support base. A motor is fixedly installed on one side wall of the support base, and the output end of the motor is fixedly connected to one end of the threaded rod.

[0010] Preferably, a guide block is fixed to the bottom of the separation cylinder, a guide rod penetrates through the guide block, and the guide rod is slidably connected to the guide block. The two ends of the guide rod are respectively fixedly connected to the two inner walls of the support base.

[0011] Preferably, the adjusting mechanism includes a guide post fixedly connected to the support plate. A sliding rod passes through the inside of the guide post and is slidably connected to the guide post. A pressing block is fixed to the top of the sliding rod, a first elastic member is fixed to the bottom of the pressing block, and the lower end of the first elastic member is fixedly connected to the top of the support plate. A pin rod penetrates through the side wall of the guide post. One end of the pin rod is fixed with a stop block, a second elastic member is fixed to the side wall of the stop block, and the other end of the second elastic member is fixedly connected to the side wall of the guide post. The end of the pin rod away from the stop block is in contact with the side wall of the sliding rod. A first pin slot and a second pin slot adapted to the pin rod are provided on the side wall of the sliding rod.

[0012] Preferably, the releasing mechanism includes a connecting plate fixedly connected to the side wall of the separation cylinder. One end of the connecting plate away from the separation cylinder is fixed with an extraction cylinder. A piston is slidably connected inside the extraction cylinder. A liquid inlet pipe is connected to the side wall of the extraction cylinder below the piston, and the other end of the liquid inlet pipe communicates with the separation cylinder. A liquid outlet pipe is connected to the bottom of the extraction cylinder. One-way valves are provided on both the liquid inlet pipe and the liquid outlet pipe. The piston is connected with an extrusion assembly for extruding the piston.

[0013] Preferably, the extrusion assembly includes a support rod fixedly connected to the top of the piston. The support rod penetrates through the top of the extraction cylinder and is slidably connected to the top of the extraction cylinder. A connecting block is fixed to the top of the support rod, a first magnet is fixed to the top of the connecting block, a second magnet is fixed to the bottom of the sliding rod, and the second magnet has the same magnetic polarity as the first magnet. A third elastic member is fixed to the bottom of the connecting block, and the lower end of the third elastic member is fixedly connected to the top of the extraction cylinder.

[0014] Preferably, the vibration mechanism includes movable rods fixedly connected to the bottom of the mesh plate and symmetrically distributed about the main shaft. Limiting plates symmetrically distributed about the main shaft are fixed on the inner wall of the separation cylinder. The movable rods penetrate through the limiting plates and are slidably connected to the limiting plates. Fourth elastic members are arranged outside the movable rods, and two ends of the fourth elastic members are respectively fixedly connected to the top of the limiting plates and the bottom of the mesh plate. A rotating rod penetrates through the side wall of the separation cylinder, and the rotating rod is rotatably connected to the side wall of the separation cylinder. A turntable is fixed at one end of the rotating rod outside the separation cylinder. A push rod arranged eccentrically is fixed on the side wall of the turntable. A pushing assembly is connected to the sliding rod, and the pushing assembly is used to push the push rod, so that the push rod drives the rotating rod to rotate through the turntable. Winding rollers symmetrically distributed about the main shaft are fixed on the outside of the rotating rod. The winding rollers are fixedly connected with traction ropes, and the other ends of the traction ropes are fixedly connected to the bottoms of the movable rods.

[0015] Preferably, the pushing assembly includes a fixing plate fixedly connected to the side wall of the sliding rod. A bracket capable of pushing the push rod is fixed to the bottom of the fixing plate. The bracket has a bent portion.

[0016] Preferably, a fixing strip is fixed between the inner walls of the support base. A plurality of drainage grooves evenly distributed are penetrated inside the fixing strip, and the drainage grooves are fixedly connected to the fixing strip. The plurality of drainage grooves are respectively located directly above the plurality of culture media.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: When the device separates and extracts microorganisms, the main shaft drives the stirring blades to rotate, so as to stir the microorganism sample and the diluent, and further make the microorganism sample evenly disperse inside the diluent, reducing the concentration of microorganisms in the sample and facilitating the separation of single colonies and counting.

[0018] The device filters the microorganism sample through the mesh plate to remove large particle impurities in the microorganism sample and retain the microorganisms. While the main shaft rotates, the sliding rod is driven to move in a circular motion through the support plate. While the sliding rod moves in a circular motion, the push rod is pushed by the bracket, so that the push rod drives the rotating rod to rotate through the turntable. The rotating rod pulls the traction rope through the winding roller, and the traction rope drives the mesh plate to move downward through the movable rod. As the sliding rod moves in a circular motion, the push rod will move below the bracket. At this time, the bracket no longer presses the push rod, and the mesh plate automatically moves upward. This cycle is repeated, so that the mesh plate can continuously vibrate up and down, thereby reducing blockage and improving the filtering efficiency of the microorganism sample.

[0019] After diluting the microbial sample, the device moves the sliding rod to the lowest point. When the sliding rod rotates above the extraction cylinder, the second magnet repels the first magnet, causing the first magnet to drive the piston downward through the support rod. The piston moves downward to squeeze the diluted microbial sample below it, and the microbial sample is discharged into the culture medium through the liquid outlet pipe. When the second magnet moves to the other side of the first magnet, the second magnet no longer repels the first magnet, and the connecting block drives the piston upward through the support rod. A negative pressure is formed below the piston, and the diluted microbial sample inside the separation cylinder enters the extraction cylinder through the liquid inlet pipe. And in cooperation with the horizontal movement of the separation cylinder, the diluted microbial sample inside the extraction cylinder can enter different culture media, eliminating the need for manual transfer of the pretreated microbial sample into different culture media, effectively improving the separation and culture efficiency of the microbial sample;

[0020] When the sliding rod is at the lowest point, the bent part of the bracket is on the same horizontal plane as the push rod. When the bracket rotates to the position of the push rod, the push rod will directly pass through the inside of the bent part, preventing the bracket from pushing the push rod and thus preventing the net plate from moving downward, reducing the friction frequency between the net plate and the inner wall of the separation cylinder and increasing the service life of the net plate. Brief Description of the Drawings

[0021] Figure 1 It is a schematic diagram of the overall structure of the extraction device in the embodiment of the present invention.

[0022] Figure 2 It is a schematic diagram of the connection structure between the moving block and the threaded rod in the embodiment of the present invention.

[0023] Figure 3 It is a schematic diagram of the external structure of the separation cylinder in the embodiment of the present invention.

[0024] Figure 4 It is a cross-sectional view of the internal structure of the separation cylinder in the embodiment of the present invention.

[0025] Figure 5 It is a schematic diagram of the bracket structure in the embodiment of the present invention.

[0026] Figure 6 It is a schematic diagram of the internal structure of the extraction cylinder in the embodiment of the present invention.

[0027] Figure 7 It is a schematic diagram of the internal structure of the guide post in the embodiment of the present invention.

[0028] In the figure: 1 - support base; 2 - rotating mechanism; 21 - gear; 22 - toothed plate; 3 - translation mechanism; 31 - motor; 32 - moving block; 33 - threaded rod; 34 - guide block; 35 - guide rod; 4 - adjusting mechanism; 41 - pressing block; 42 - first elastic member; 43 - guide post; 44 - first pin slot; 45 - second pin slot; 46 - pin rod; 47 - second elastic member; 48 - stop block; 5 - releasing mechanism; 51 - first magnet; 52 - second magnet; 53 - connecting block; 54 - support rod; 55 - third elastic member; 56 - extraction cylinder; 57 - piston; 58 - liquid inlet pipe; 59 - liquid outlet pipe; 6 - vibration mechanism; 61 - fourth elastic member; 62 - limiting plate; 63 - movable rod; 64 - towing rope; 65 - winding roller; 66 - rotating rod; 67 - turntable; 68 - push rod; 69 - support; 610 - bending part; 611 - fixing plate; 7 - separation cylinder; 8 - fixing strip; 9 - drainage groove; 10 - culture medium; 11 - main shaft; 12 - support plate; 13 - slide bar; 14 - fixing rod; 15 - guide cylinder; 16 - mesh plate; 17 - stirring blade. Detailed implementation manners

[0029] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] The specific implementation of the present invention will be described in detail below with reference to specific embodiments.

[0031] In one embodiment, please refer to Figures 1-4, a microorganism separation and extraction device, comprising a support base 1 and a separation cylinder 7. The top of the separation cylinder 7 is penetrated by a main shaft 11, and the main shaft 11 is rotatably connected to the top of the separation cylinder 7. A mesh plate 16 is arranged inside the separation cylinder 7, and the side wall of the mesh plate 16 is slidably connected to the inner wall of the separation cylinder 7. The mesh plate 16 is connected with a vibration mechanism 6, and the vibration mechanism 6 is used to drive the mesh plate 16 to vibrate up and down. A guide cylinder 15 penetrates through the mesh plate 16. A fixing rod 14 is fixed on the outer wall of the guide cylinder 15, and the other end of the fixing rod 14 is fixedly connected to the inner wall of the separation cylinder 7. The outer wall of the guide cylinder 15 is slidably connected to the inner wall of the mesh plate 16. The main shaft 11 penetrates through the guide cylinder 15 and is rotatably connected to the guide cylinder 15. A plurality of stirring blades 17 are fixed on the outer part of the main shaft 11. The separation cylinder 7 is connected to the support base 1 through a translation mechanism 3, and the translation mechanism 3 is used to drive the separation cylinder 7 to move horizontally. Among them, the separation cylinder 7 is connected with a release mechanism 5. A plurality of placement grooves are equidistantly distributed at the bottom of the support base 1, and a culture medium 10 is placed inside the placement grooves. The release mechanism 5 is used to discharge the sample inside the separation cylinder 7 into the culture medium 10. A support plate 12 is fixed on the side wall of the main shaft 11. A sliding rod 13 penetrates through the support plate 12, and the sliding rod 13 is slidably connected to the support plate 12. The sliding rod 13 is connected with an adjustment mechanism 4, and the adjustment mechanism 4 is used to adjust the height of the sliding rod 13, so that the sliding rod 13 controls the operation of the vibration mechanism 6 and the release mechanism 5.

[0032] In this embodiment, when the device separates and extracts microorganisms, a certain amount of diluent is poured into the inside of the separation cylinder 7. The diluent can be a sterile buffer solution or physiological saline. Then, the microorganism sample is poured into the inside of the separation cylinder 7. Subsequently, the translation mechanism 3 drives the separation cylinder 7 to move horizontally. While the separation cylinder 7 moves horizontally, the rotation mechanism 2 drives the main shaft 11 to rotate, and the main shaft 11 drives the stirring blade 17 to rotate. The mixing liquid of the diluent and the microorganism sample is mixed and stirred by the stirring blade 17, so that the microorganism sample can be evenly dispersed in the diluent, reducing the concentration of microorganisms in the sample and facilitating the separation and counting of single colonies. A mesh plate 16 is provided inside the separation cylinder 7, which can play a filtering role on the microorganism sample, thereby removing large particle impurities in the microorganism sample and retaining the microorganisms. At the same time, when filtering the microorganism sample, the vibration mechanism 6 can drive the mesh plate 16 to vibrate up and down, thereby reducing blockage and improving the filtering efficiency of the microorganism sample. The setting of the guide cylinder 15 can effectively improve the stability between the mesh plate 16 and the main shaft 11. After the microorganism sample is diluted, the height of the sliding rod 13 is adjusted by the adjustment mechanism 4, so that on the one hand, the sliding rod 13 can drive the release mechanism 5 to operate. When the release mechanism 5 moves above the culture medium 10, the release mechanism 5 automatically releases the microorganism sample inside the separation cylinder 7 into the culture medium 10. Different specific inhibitors or nutrients can be added to the culture medium 10, allowing only the target microorganisms to grow, thereby achieving the purpose of separating and culturing microorganisms. On the other hand, the vibration mechanism 6 no longer drives the mesh plate 16 to vibrate up and down, thereby reducing the friction frequency between the mesh plate 16 and the inner wall of the separation cylinder 7 and improving the service life of the mesh plate 16.

[0033] Please refer to Figure 2 and Figure 3 The rotation mechanism 2 includes a gear 21 fixed to the top of the main shaft 11, and a toothed plate 22 is fixed between the inner walls of the support seat 1. The toothed plate 22 meshes with the gear 21;

[0034] When separating and extracting microorganisms, the translation mechanism 3 drives the separation cylinder 7 to move horizontally. The separation cylinder 7 drives the gear 21 to move horizontally through the main shaft 11. While the gear 21 moves horizontally, it will mesh with the toothed plate 22, thereby causing the main shaft 11 to rotate. The main shaft 11 drives the stirring blade 17 to rotate to stir the microorganism sample and the diluent, so that the microorganism sample can be evenly dispersed in the diluent, reducing the concentration of microorganisms in the sample and facilitating the separation and counting of single colonies.

[0035] Please refer to Figure 1 and Figure 2, the translation mechanism 3 includes a moving block 32 fixed to the bottom of the separation cylinder 7. A threaded rod 33 passes through the interior of the moving block 32. The threaded rod 33 is threadedly connected to the moving block 32. The two ends of the threaded rod 33 are respectively rotatably connected to the two inner walls of the support base 1. A motor 31 is fixedly installed on one side wall of the support base 1. The output end of the motor 31 is fixedly connected to one end of the threaded rod 33;

[0036] When separating and extracting microorganisms, start the motor 31. The motor 31 drives the threaded rod 33 to rotate. Through the threaded connection between the threaded rod 33 and the moving block 32, the separation cylinder 7 is driven to move horizontally, so that the release mechanism 5 can pass above multiple culture media 10 in sequence. Furthermore, the microbial samples inside the culture media 10 can enter different culture media 10, effectively improving the efficiency of microbial separation and culture.

[0037] Please refer to Figure 2 , a guide block 34 is fixed to the bottom of the separation cylinder 7. A guide rod 35 passes through the interior of the guide block 34. The guide rod 35 is slidably connected to the guide block 34. The two ends of the guide rod 35 are respectively fixedly connected to the two inner walls of the support base 1;

[0038] When the separation cylinder 7 moves, it drives the guide block 34 to slide outside the guide rod 35. The guide rod 35 plays a limiting role on the separation cylinder 7 through the guide block 34, effectively improving the stability of the separation cylinder 7 during movement.

[0039] Please refer to Figure 7 , the adjustment mechanism 4 includes a guide post 43 fixedly connected to the support plate 12. The slide rod 13 passes through the interior of the guide post 43 and is slidably connected to the guide post 43. The top of the slide rod 13 is fixedly provided with a pressing block 41. A first elastic member 42 is fixed to the bottom of the pressing block 41. The lower end of the first elastic member 42 is fixedly connected to the top of the support plate 12. A pin rod 46 passes through the side wall of the guide post 43. One end of the pin rod 46 is fixedly provided with a stop block 48. A second elastic member 47 is fixed to the side wall of the stop block 48. The other end of the second elastic member 47 is fixedly connected to the side wall of the guide post 43. The end of the pin rod 46 away from the stop block 48 is in contact with the side wall of the slide rod 13. A first pin slot 44 and a second pin slot 45 adapted to the pin rod 46 are provided on the side wall of the slide rod 13;

[0040] When diluting a microbial sample, the end of the pin rod 46 is located inside the second pin slot 45 under the action of the stop block 48 and the second elastic member 47. The slide rod 13 is fixed by the pin rod 46. At this time, the slide rod 13 is at the highest point, and the release mechanism 5 does not release the microbial sample. At the same time, the vibration mechanism 6 drives the mesh plate 16 to vibrate up and down. When the microbial dilution is completed, the pin rod 46 is pulled outwards, causing the pin rod 46 to move outside the second pin slot 45. Then, the pressing block 41 is pressed, and the pressing block 41 drives the slide rod 13 to move downwards. When the first pin slot 44 on the side wall of the slide rod 13 aligns with the pin rod 46, the end of the pin rod 46 automatically enters the first pin slot 44 under the action of the second elastic member 47. At this time, the slide rod 13 is at the lowest point, and the release mechanism 5 can discharge the microbial sample into different culture media 10. At the same time, the vibration mechanism 6 no longer drives the mesh plate 16 to vibrate. The first elastic member 42 can play a reset role for the slide rod 13 through the pressing block 41. Both the first elastic member 42 and the second elastic member 47 can be springs.

[0041] Please refer to Figure 6 As shown in the figure, the release mechanism 5 includes a connecting plate fixedly connected to the side wall of the separation cylinder 7. One end of the connecting plate away from the separation cylinder 7 is fixedly provided with a pumping cylinder 56. A piston 57 is slidably connected inside the pumping cylinder 56. Below the piston 57, a liquid inlet pipe 58 is connected to the side wall of the pumping cylinder 56. The other end of the liquid inlet pipe 58 communicates with the separation cylinder 7. The bottom of the pumping cylinder 56 is connected with a liquid outlet pipe 59. One-way valves are provided on both the liquid inlet pipe 58 and the liquid outlet pipe 59. The piston 57 is connected with an extrusion assembly for extruding the piston 57.

[0042] While the main shaft 11 rotates, it also drives the slide rod 13 to move in a circular motion through the support plate 12. After the microbial dilution is completed, the slide rod 13 is adjusted to the lowest point through the adjustment mechanism 4. At this time, when the slide rod 13 rotates above the pumping cylinder 56, the slide rod 13 extrudes the piston 57 through the extrusion assembly, causing the piston 57 to move downwards. The piston 57 moves downwards to extrude the diluted microbial sample below it. Under the action of the one-way valve, the microbial sample is discharged into the culture medium 10 through the liquid outlet pipe 59. When the slide rod 13 moves to the other side of the pumping cylinder 56, the extrusion no longer acts on the piston 57, and the piston 57 automatically resets. A negative pressure is formed below the piston 57. Under the action of the one-way valve, the diluted microbial sample inside the separation cylinder 7 enters the pumping cylinder 56 through the liquid inlet pipe 58. This cycle repeats. Whenever the slide rod 13 rotates above the pumping cylinder 56, the diluted microbial sample inside the pumping cylinder 56 will be discharged from the liquid outlet pipe 59. And in cooperation with the movement of the separation cylinder 7, the diluted microbial sample inside the pumping cylinder 56 can enter different culture media 10, effectively improving the separation and culture efficiency of the microbial sample.

[0043] Please refer to Figure 6, the extrusion assembly includes a support rod 54 fixedly connected to the top of the piston 57. The support rod 54 penetrates through the top of the extraction cylinder 56 and is slidably connected to the top of the extraction cylinder 56. A connecting block 53 is fixedly installed at the top of the support rod 54, and a first magnet 51 is fixedly installed at the top of the connecting block 53. A second magnet 52 is fixedly installed at the bottom of the sliding rod 13. The second magnet 52 has the same magnetic property as the first magnet 51. A third elastic member 55 is fixedly installed at the bottom of the connecting block 53, and the lower end of the third elastic member 55 is fixedly connected to the top of the extraction cylinder 56;

[0044] When diluting the microorganisms, the sliding rod 13 is at the highest point. At this time, even if the sliding rod 13 moves above the extraction cylinder 56, the second magnet 52 at the bottom of the sliding rod 13 will not repel the first magnet 51, so that the piston 57 will not move downward and squeeze the diluted microorganism sample below it. After the microorganism dilution is completed, the sliding rod 13 is adjusted to the lowest point by the adjusting mechanism 4. At this time, when the sliding rod 13 rotates above the extraction cylinder 56, the second magnet 52 repels the first magnet 51, so that the first magnet 51 drives the piston 57 to move downward through the support rod 54. When the second magnet 52 moves to the other side of the first magnet 51, the second magnet 52 no longer repels the first magnet 51, and the connecting block 53 drives the piston 57 to move upward through the support rod 54 under the action of the third elastic member 55. The third elastic member 55 can be a spring.

[0045] Please refer to Figure 4 and Figure 5 , the vibration mechanism 6 includes movable rods 63 fixedly connected to the bottom of the mesh plate 16 and symmetrically distributed about the main shaft 11. Limit plates 62 symmetrically distributed about the main shaft 11 are fixedly installed on the inner wall of the separation cylinder 7. The movable rods 63 penetrate through the limit plates 62 and are slidably connected to the limit plates 62. Fourth elastic members 61 are arranged outside the movable rods 63, and both ends of the fourth elastic members 61 are fixedly connected to the top of the limit plates 62 and the bottom of the mesh plate 16 respectively. A rotating rod 66 penetrates through the side wall of the separation cylinder 7, and the rotating rod 66 is rotatably connected to the side wall of the separation cylinder 7. A turntable 67 is fixedly installed at one end of the rotating rod 66 outside the separation cylinder 7, and an eccentric push rod 68 is fixedly installed on the side wall of the turntable 67. A pushing assembly is connected to the sliding rod 13, and the pushing assembly is used to push the push rod 68, so that the push rod 68 drives the rotating rod 66 to rotate through the turntable 67. Winding rollers 65 symmetrically distributed about the main shaft 11 are fixedly installed on the outside of the rotating rod 66, and traction ropes 64 are fixedly connected to the winding rollers 65. The other ends of the traction ropes 64 are fixedly connected to the bottoms of the movable rods 63;

[0046] When diluting microorganisms, the slide bar 13 is at the highest point. At this time, while the slide bar 13 makes a circular motion, it pushes the push rod 68 through the pushing component. As a result, the push rod 68 drives the rotating rod 66 to rotate through the turntable 67. The rotating rod 66 pulls the traction rope 64 through the winding roller 65, and the traction rope 64 drives the net plate 16 to move downward through the movable rod 63. As the slide bar 13 makes a circular motion, when the pushing component no longer pushes the push rod 68, the net plate 16 automatically moves upward under the action of the fourth elastic member 61. This cycle is repeated, enabling the net plate 16 to continuously vibrate up and down, thereby reducing blockage and improving the filtration efficiency of the microorganism sample. The fourth elastic member 61 can be a spring.

[0047] Please refer to Figure 5 As shown in the figure, the pushing component includes a fixing plate 611 fixedly connected to the side wall of the slide bar 13. A bracket 69 capable of pushing the push rod 68 is fixedly installed at the bottom of the fixing plate 611, and the bracket 69 has a bent portion 610.

[0048] When diluting microorganisms, the slide bar 13 is at the highest point. At this time, while the slide bar 13 makes a circular motion, it pushes the push rod 68 through the bracket 69, and then the push rod 68 drives the rotating rod 66 to rotate through the turntable 67. As the slide bar 13 makes a circular motion, the push rod 68 will move below the bracket 69. At this time, the bracket 69 no longer presses the push rod 68, and the net plate 16 automatically moves upward under the action of the fourth elastic member 61. When the dilution of microorganisms is completed, the slide bar 13 is adjusted to the lowest point through the adjusting mechanism 4. At this time, the bent portion 610 of the bracket 69 and the push rod 68 are on the same horizontal plane. When the bracket 69 rotates to the position of the push rod 68, the push rod 68 will directly pass through the inside of the bent portion 610, so that the bracket 69 cannot push the push rod 68, and then the net plate 16 will not move downward, reducing the friction frequency between the net plate 16 and the inner wall of the separation cylinder 7 and improving the service life of the net plate 16.

[0049] Please refer to Figure 1 As shown in the figure, a fixing bar 8 is fixedly installed between the inner walls of the support base 1. A plurality of drainage grooves 9 are equidistantly distributed through the fixing bar 8, and the drainage grooves 9 are fixedly connected to the fixing bar 8. Among them, the plurality of drainage grooves 9 are respectively located directly above the plurality of culture media 10.

[0050] The arrangement of the drainage grooves 9 can play a guiding role for the diluted microorganism sample, so that the diluted microorganism sample can accurately flow into different culture media 10.

[0051] Working principle: When the device separates and extracts microorganisms, first, a certain amount of diluent is poured into the inside of the separation cylinder 7, and then the microorganism sample is poured into the inside of the separation cylinder 7. Subsequently, the motor 31 is started. The motor 31 drives the threaded rod 33 to rotate. Through the threaded connection between the threaded rod 33 and the moving block 32, the separation cylinder 7 is driven to move horizontally. While the separation cylinder 7 moves horizontally, the main shaft 11 drives the gear 21 to move horizontally through the main shaft 11. While the gear 21 moves horizontally, it meshes with the toothed plate 22, thereby causing the main shaft 11 to rotate. The main shaft 11 drives the stirring blade 17 to rotate, thus stirring the microorganism sample and the diluent, so that the microorganism sample can be evenly dispersed in the diluent, reducing the concentration of microorganisms in the sample, facilitating the separation of single colonies and counting. A mesh plate 16 is provided inside the separation cylinder 7, and the mesh plate 16 can play a filtering role on the microorganism sample, thereby removing large particle impurities in the microorganism sample and retaining the microorganisms. In addition, while the main shaft 11 rotates, the support plate 12 drives the slide rod 13 to move in a circular motion. While the slide rod 13 moves in a circular motion, the bracket 69 is pushed to drive the push rod 68. Then, the push rod 68 drives the rotating rod 66 to rotate through the turntable 67. The rotating rod 66 pulls the traction rope 64 through the winding roller 65. The traction rope 64 drives the mesh plate 16 to move downward through the movable rod 63. As the slide rod 13 moves in a circular motion, the push rod 68 will move below the bracket 69. At this time, the bracket 69 no longer presses the push rod 68, and the mesh plate 16 automatically moves upward under the action of the fourth elastic member 61. This cycle is repeated, so that the mesh plate 16 can continuously vibrate up and down, thereby reducing blockage and improving the filtering efficiency of the microorganism sample. After the microorganism sample is diluted, the pin rod 46 is pulled outwards, so that the pin rod 46 moves outside the second pin slot 45. Then, the pressing block 41 is pressed. The pressing block 41 drives the slide rod 13 to move downward. When the first pin slot 44 on the side wall of the slide rod 13 is aligned with the pin rod 46, the end of the pin rod 46 automatically enters the first pin slot 44 under the action of the second elastic member 47. At this time, the slide rod 13 is at the lowest point. When the slide rod 13 rotates above the extraction cylinder 56, the second magnet 52 repels the first magnet 51, so that the first magnet 51 drives the piston 57 to move downward through the support rod 54. The piston 57 moves downward to squeeze the diluted microorganism sample below it. Under the action of the one-way valve, the microorganism sample is discharged into the culture medium 10 through the liquid outlet pipe 59. When the second magnet 52 moves to the other side of the first magnet 51, the second magnet 52 and the first magnet 51 no longer repel each other. The connecting block 53 drives the piston 57 to move upward through the support rod 54 under the action of the third elastic member 55. A negative pressure is formed below the piston 57. Under the action of the one-way valve, the diluted microorganism sample inside the separation cylinder 7 enters the extraction cylinder 56 through the liquid inlet pipe 58. This cycle is repeated, so that the diluted microorganism sample inside the extraction cylinder 56 can enter different culture media 10, effectively improving the separation and culture efficiency of the microorganism sample. In addition, when the slide rod 13 is at the lowest point,The bent portion 610 of the bracket 69 is on the same horizontal plane as the push rod 68. When the bracket 69 rotates to the position of the push rod 68, the push rod 68 will directly pass through the inside of the bent portion 610, so that the bracket 69 cannot push the push rod 68, and further the net plate 16 will not move downward, reducing the friction frequency between the net plate 16 and the inner wall of the separation cylinder 7 and improving the service life of the net plate 16.

[0052] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A microorganism separation and extraction device, comprising a support seat and a separation cylinder; characterized in that: The top of the separation cylinder is penetrated by a main shaft, and the main shaft is rotatably connected to the top of the separation cylinder, a mesh plate is arranged inside the separation cylinder, and the side wall of the mesh plate is slidably connected to the inner wall of the separation cylinder, and the mesh plate is connected to a vibration mechanism, and the vibration mechanism is used to drive the mesh plate to vibrate up and down, and a guide cylinder is penetrated inside the mesh plate, and a fixing rod is fixed on the outer wall of the guide cylinder, and the other end of the fixing rod is fixedly connected to the inner wall of the separation cylinder and is slidably connected to the inner wall of the mesh plate, the main shaft penetrates the guide cylinder and is rotatably connected to the guide cylinder, and a plurality of stirring blades are fixed outside the main shaft, and the separation cylinder is connected to the support seat through a translation mechanism, and the translation mechanism is used to drive the separation cylinder to move horizontally, wherein the separation cylinder is connected to a release mechanism, and a plurality of placement grooves distributed equidistantly are provided at the bottom of the support seat, and a culture medium is placed in the placement groove, and the release mechanism is used to discharge the sample inside the separation cylinder into the culture medium, and a support plate is fixed on the side wall of the main shaft, and a sliding rod is penetrated inside the support plate, and the sliding rod is slidably connected to the support plate, and the sliding rod is connected to an adjustment mechanism, and the adjustment mechanism is used to adjust the height of the sliding rod, so that the sliding rod controls the operation of the vibration mechanism and the release mechanism.

2. A microorganism separation and extraction device according to claim 1, characterized in that: The rotating mechanism comprises a gear fixed on the top of the main shaft, and a toothed plate is fixed between the inner walls of the support seat, and the toothed plate is meshed with the gear.

3. A microorganism separation and extraction device according to claim 1, characterized in that: The translation mechanism includes a moving block fixed at the bottom of the separation cylinder, a threaded rod passes through the inside of the moving block, the threaded rod is threadedly connected to the moving block, both ends of the threaded rod are rotatably connected to the two inner walls of the support seat, a motor is fixedly installed on one of the side walls of the support seat, and the output end of the motor is fixedly connected to one end of the threaded rod.

4. A microorganism separation and extraction device according to claim 3, characterized in that: A guide block is fixed at the bottom of the separation cylinder, a guide rod penetrates the inside of the guide block, the guide rod is slidably connected to the guide block, and two ends of the guide rod are respectively fixedly connected to two inner walls of the support seat.

5. The microorganism separation and extraction device according to claim 1, characterized in that: The adjusting mechanism includes a guide column fixedly connected to the support plate, a sliding rod passing through the inside of the guide column and slidably connected to the guide column, wherein a pressure block is fixed on the top of the sliding rod, a first elastic member is fixed on the bottom of the pressure block, the lower end of the first elastic member is fixedly connected to the top of the support plate, a pin rod passes through the side wall of the guide column, a block is fixed at one end of the pin rod, a second elastic member is fixed on the side wall of the block, the other end of the second elastic member is fixedly connected to the side wall of the guide column, the end of the pin rod away from the block is in contact with the side wall of the sliding rod, wherein the side wall of the sliding rod is provided with a first pin groove and a second pin groove matched with the pin rod.

6. The microorganism separation and extraction device according to claim 1, characterized in that: The release mechanism includes a connecting plate fixedly connected to the side wall of the separation cylinder, an extraction cylinder is fixed to one end of the connecting plate away from the separation cylinder, a piston is slidably connected inside the extraction cylinder, a liquid inlet pipe is connected below the piston and on the side wall of the extraction cylinder, the other end of the liquid inlet pipe is connected to the separation cylinder, a liquid outlet pipe is connected to the bottom of the extraction cylinder, both the liquid inlet pipe and the liquid outlet pipe are provided with a one-way valve, and the piston is connected to an extrusion assembly, which is used to squeeze the piston.

7. A microorganism separation and extraction device according to claim 6, characterized in that: The extrusion assembly includes a support rod fixedly connected to the top of the piston, the support rod passes through the top of the extraction cylinder and is slidably connected to the top of the extraction cylinder, a connecting block is fixed to the top of the support rod, a first magnet is fixed to the top of the connecting block, a second magnet is fixed to the bottom of the sliding rod, the second magnet has the same magnetic property as the first magnet, a third elastic member is fixed to the bottom of the connecting block, and the lower end of the third elastic member is fixedly connected to the top of the extraction cylinder.

8. The microorganism separation and extraction device according to claim 1, characterized in that: The cam is provided with a plurality of movable members, each of which is provided with a plurality of movable members, and the movable members are provided with a plurality of movable members at the plurality of movable members.

9. A microorganism separation and extraction device according to claim 8, characterized in that: The pushing assembly comprises a fixing plate fixedly connected to the side wall of the sliding rod, a bracket capable of pushing the pushing rod is fixed at the bottom of the fixing plate, wherein the bracket has a bent portion.

10. The microorganism separation and extraction device according to claim 1, characterized in that: A fixing bar is fixed between the inner walls of the support seat, and a plurality of drainage grooves distributed at equal intervals are penetrated inside the fixing bar. The drainage grooves are fixedly connected to the fixing bar, wherein the plurality of drainage grooves are respectively located directly above the plurality of culture media.

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