Microbial fermentation strain extraction and detection device for fermented beverage

Abstract

The invention relates to the technical field of strain extraction, in particular to a microbial fermentation strain extraction and detection device for a fermented beverage. Comprising a tank body, and a tank cover is fixedly mounted at the upper end of the tank body; a feeding hole communicated with the tank body is formed in the upper end of the tank cover; by arranging the partition plate, the interior of the tank body is divided into two independent cavities by the partition plate, so that a sampler firstly enters the cavity above the partition plate, and infectious microbes in external air are sterilized when entering the cavity above the partition plate through the sampling port; then the sterilized sampler samples the strains cultured in the chamber below the partition plate through the U-shaped pipe, so that infectious microbes suspended in the air above are prevented from directly falling into the chamber below the partition plate, and the risk that the strains in the chamber below are contaminated is reduced; the safety of strain extraction is guaranteed, the extracted strains are prevented from being polluted by infectious microbes in the air, the fluctuation range of the detection result is reduced, and the detection data are more accurate.

Description

Technical Field

[0001] The invention relates to the technical field of strain extraction, in particular to a device for extracting and detecting microbial fermentation strains of fermented beverages. Background Art

[0002] Bacteria are the foundation of the fermentation industry. Without bacteria, microbial fermentation cannot be carried out. Commonly used microbial fermentation bacteria are yeast, lactic acid bacteria and acetic acid bacteria. In fermented beverages, yeast produces ethanol and carbon dioxide by fermenting sugars, and is widely used in the production of foods such as wine and yogurt. Lactic acid bacteria and acetic acid bacteria are used in the fermentation of dairy beverages, and during the fermentation process of dairy beverages, they increase the sourness of the fermented beverages, giving the beverages a unique sour taste and flavor. During the cultivation process, yeast, lactic acid bacteria, acetic acid bacteria and other strains can be divided into preserved strains, shake flask strains and seed tank strains according to the cultivation stage; the preserved strains are the backup of the production strains, usually stored in a low-temperature dry state; shake flask strains are used to activate the preserved strains; seed tank strains are used to inoculate fermentation tanks; before the preserved strains enter the production inoculation, they need to be activated before use to convert them from a dormant state to a normal metabolic state; the activated strains are then expanded, and at this time they need to be cultured in shake flasks (eggplant bottles, etc.), and the culture medium used at this time is more extensive and more economical than the culture medium used for preserved strains; when the shake flask seeds are further expanded, they need to be cultured in seed tanks; the culture medium in the seed tank is closer to the composition of the culture medium used in the fermentation tank, and the purpose is to allow the strains to further adapt to the environment of the fermentation medium; However, during the strain culture stage, it is often necessary to extract and test the cultured strains to prevent the strains from being contaminated during the culture period. If the cultured strains are contaminated by foreign bacteria, the foreign bacteria will compete with the target strains for nutrients, living space and other resources during the fermentation process, affecting the normal growth and metabolism of the target strains, thereby reducing the yield of the fermentation product. Therefore, once the seeds are found to be contaminated, they should be sterilized and discarded immediately, and the seed tank and its pipelines should also be thoroughly cleaned. When extracting bacteria in the culture stage, a certain amount of culture fluid is usually sucked from the sampling port of the culture container (such as a seed tank) using a sterile pipette or pipette as a sample; although the sampling operation is usually carried out in a relatively clean environment, the sampling port and its connected pipe parts are strictly steam sterilized before the sampling operation; however, it is unavoidable that various bacteria spores may still be suspended in the surrounding air; when the sampling port is opened for sampling, the bacteria suspended in the air will fall into the sampling port, thereby causing bacterial contamination hazards to the bacteria in the seed tank; In view of this, in order to overcome the above technical problems, the present invention proposes a device for extracting and detecting microbial fermentation strains of fermented beverages, which solves the above technical problems. Summary of the invention

[0003] In order to make up for the deficiencies of the prior art, the present invention proposes a device for extracting and detecting microbial fermentation strains of a fermented beverage. By setting a partition plate, the interior of the tank body is divided into two independent chambers by the partition plate, so that the sampler first enters the chamber above the partition plate. When miscellaneous bacteria in the outside air enter the chamber above the partition plate through the sampling port, the miscellaneous bacteria will be sterilized. Then, the sterilized sampler samples the strains cultured in the chamber below the partition plate through a U-shaped tube, thereby preventing the miscellaneous bacteria suspended in the air above from directly falling into the chamber below the partition plate, reducing the risk of the strains in the lower chamber being contaminated with bacteria; ensuring the safety of strain extraction, and at the same time preventing the extracted strains from being contaminated by miscellaneous bacteria in the air, reducing the fluctuation range of the detection results and making the detection data more accurate.

[0004] The technical solution adopted by the present invention to solve its technical problems is as follows: A device for extracting and detecting microbial fermentation strains of a fermented beverage according to the present invention includes: A tank body, with a tank cover fixedly installed at the upper end of the tank body; a feed port communicating with the tank body is opened at the upper end of the tank cover; a discharge port is opened at the lower end of the tank body; a driving motor is fixedly installed at the upper end of the tank cover; a stirring rod is rotatably connected inside the tank body; the driving motor is used to drive the stirring rod to rotate; a temperature sensor is embedded in the inner wall of the tank body; A partition plate, which is fixedly connected to the inner wall of the tank body; the partition plate divides the interior of the tank body into two independent chambers; a through groove is opened at the upper end of the partition plate; the stirring rod is rotatably connected to the partition plate; the through groove is communicated with the feed port through a connecting pipe; a sampling port is opened on the surface of the tank cover; a U-shaped tube is arranged below the partition plate; both ends of the U-shaped tube penetrate through the partition plate; an air inlet and an air outlet are opened on the surface of the tank body; a spiral groove is opened inside the tank body; a heating pipe communicated with the spiral groove is opened on the outer surface of the tank body; A sampling module, which is installed in the U-shaped tube; the sampling module is used to sample the strains in the tank body.

[0005] Preferably, the sampling module includes: An intubation tube, which is embedded in the outer wall of the U-shaped tube; a straight tube is slidably and sealingly connected to one end of the intubation tube close to the sampler; mounting holes are opened on the side walls of the intubation tube and the straight tube; one-way valves are fixedly installed in the mounting holes; A sampler, with a mounting groove opened on the surface of the sampler; a sampling tube is slidably and sealingly connected in the mounting groove; the sampling tube and the sampler are connected by a connecting spring; an electromagnetic column is embedded inside the sampler; the number of electromagnetic columns is set to four; A conveying unit, which is installed in the U-shaped tube; the conveying unit is used to convey the sampler to move in the U-shaped tube.

[0006] Preferably, a baffle is arranged inside the pipe orifice of the sampling pipe; the baffle is rotatably connected to the inner wall of the sampling pipe through a torsion spring.

[0007] Preferably, there are three groups of the intubation tubes; the three groups of intubation tubes are distributed up and down.

[0008] Preferably, the conveying unit includes a conveyor belt; tooth grooves are formed on the inner ring wall of the conveyor belt; clamping grooves are formed on the outer ring wall of the conveyor belt; a clamping block is fixedly connected to one side of the sampler close to the clamping groove; the clamping block is inserted into the clamping groove; a magnet is embedded at the bottom of the clamping groove; a spur gear shaft is rotatably connected to the inner wall of the tank body; the spur gear shaft is in meshing transmission with the tooth grooves of the conveyor belt; the spur gear shaft is connected to the output shaft of the driving motor through a connecting unit.

[0009] Preferably, the connecting unit includes a mounting frame, and the mounting frame is fixedly connected to the inner wall of the tank body; the spur gear shaft is rotatably connected to the mounting frame; a bevel gear shaft is arranged above the spur gear shaft; the bevel gear shaft and the spur gear shaft are connected by a belt for belt transmission; a bevel gear ring is slidably connected to the surface of the output shaft of the driving motor; the bevel gear ring is connected to the output shaft of the driving motor through a first hydraulic push rod; the bevel gear ring is meshed with the bevel gear shaft.

[0010] Preferably, the connecting unit further includes a connecting rod, a rectangular groove is formed at the lower end of the output shaft of the driving motor; the connecting rod is slidably and sealingly connected in the rectangular groove; a second hydraulic push rod is arranged in the rectangular groove; one end of the second hydraulic push rod is fixedly connected to the connecting rod, and the other end is fixedly connected to the bottom of the rectangular groove; a groove is formed at one end of the stirring rod close to the driving motor; the end of the connecting rod away from the rectangular groove is slidably and sealingly connected in the groove.

[0011] Preferably, the pushing unit includes an electric push rod; one end of the electric push rod is fixedly connected to the partition plate, and the other end is fixedly connected to a rubber rod.

[0012] The beneficial effects of the present invention are as follows: By arranging the partition plate, the present invention divides the interior of the tank body into two independent chambers, so that the sampler first enters the chamber above the partition plate. When miscellaneous bacteria in the outside air enter the chamber above the partition plate through the sampling port, the miscellaneous bacteria will be sterilized. Then, the sterilized sampler samples the strains cultured in the chamber below the partition plate through the U-shaped pipe, thereby preventing the miscellaneous bacteria suspended in the air above from directly falling into the chamber below the partition plate, reducing the risk of the strains in the lower chamber being contaminated with bacteria; ensuring the safety of strain extraction, and at the same time preventing the extracted strains from being contaminated by miscellaneous bacteria in the air, reducing the fluctuation range of the detection results and making the detection data more accurate.

[0013] The present invention sets three groups of intubation tubes to collect the colony culture solution at different heights in the tank body. It can not only analyze the colony growth conditions at different heights. For example, if it is found that the colonies in the upper region of the tank grow too fast but the quality of the metabolites is poor, while the colonies in the lower region grow slowly, then according to this information, the fermentation process parameters such as the stirring speed and the heating temperature at different heights in the tank body can be adjusted accordingly, so that the colony growth and metabolism in the whole tank are more balanced, and the efficiency of fermentation production and the product quality are improved. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] The present invention will be further described below in conjunction with the drawings and embodiments.

[0015] Figure 1 is a perspective view of the present invention; Figure 2 is a partial cross-sectional view of the tank body used in the present invention; Figure 3 is Figure 2 an enlarged view of part A in Figure 4 is Figure 2 an enlarged view of part B in Figure 5 is a structural schematic diagram of the present invention; Figure 6 is Figure 5 an enlarged view of part C in In the figure: 1. Tank body; 11. Discharge port; 111. Air inlet; 112. Air outlet; 113. Spiral groove; 114. Heating pipe; 12. Stirring rod; 13. Partition board; 131. Through groove; 132. Connecting pipe; 14. U-shaped pipe; 141. Intubation tube; 142. Straight pipe; 143. Mounting hole; 15. Sampler; 151. Mounting groove; 152. Sampling pipe; 153. Connecting spring; 154. Electromagnetic column; 155. Flap; 156. Block; 16. Conveyor belt; 161. Tooth groove; 162. Card slot; 163. Magnet; 17. Straight gear shaft; 171. Mounting frame; 172. Bevel gear shaft; 173. Transmission belt; 174. Bevel gear ring; 175. First hydraulic push rod; 2. Tank cover; 21. Feed port; 22. Driving motor; 221. Connecting rod; 222. Rectangular groove; 223. Second hydraulic push rod; 224. Groove; 23. Sampling port; 24. Electric push rod; 25. Rubber rod. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0016] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0017] Such as Figures 1 to 6As shown in the figure, a device for extracting and detecting microbial fermentation strains of a fermented beverage according to the present invention includes: A tank body 1, with a tank cover 2 fixedly installed at the upper end of the tank body 1; a feed port 21 communicating with the tank body 1 is opened at the upper end of the tank cover 2; a discharge port 11 is opened at the lower end of the tank body 1; a driving motor 22 is fixedly installed at the upper end of the tank cover 2; a stirring rod 12 is rotatably connected inside the tank body 1; the driving motor 22 is used to drive the stirring rod 12 to rotate; a temperature sensor is embedded in the inner wall of the tank body 1; A partition plate 13, which is fixedly connected to the inner wall of the tank body 1; the partition plate 13 divides the interior of the tank body 1 into two independent chambers; a through groove 131 is opened at the upper end of the partition plate 13; the stirring rod 12 is rotatably connected to the partition plate 13; the through groove 131 is communicated with the feed port 21 through a connecting pipe 132; a sampling port 23 is opened on the surface of the tank cover 2; a U-shaped pipe 14 is arranged below the partition plate 13; both ends of the U-shaped pipe 14 penetrate through the partition plate 13; an air inlet 111 and an air outlet 112 are opened on the surface of the tank body 1; a spiral groove 113 is opened inside the tank body 1; a heating pipe 114 communicated with the spiral groove 113 is opened on the outer surface of the tank body 1; A sampling module, which is installed in the U-shaped pipe 14; the sampling module is used to sample the strains in the tank body 1.

[0018] As an embodiment of the present invention, the sampling module includes: An insertion tube 141, which is embedded in the outer wall of the U-shaped pipe 14; a straight tube 142 is slidably and sealingly connected to one end of the insertion tube 141 close to the sampler 15; mounting holes 143 are opened on the side walls of both the insertion tube 141 and the straight tube 142; one-way valves are fixedly installed in the mounting holes 143; A sampler 15, with a mounting groove 151 opened on the surface of the sampler 15; a sampling tube 152 is slidably and sealingly connected in the mounting groove 151; the sampling tube 152 is connected to the sampler 15 through a connecting spring 153; an electromagnetic column 154 is embedded inside the sampler 15; the number of the electromagnetic columns 154 is set to four; A conveying unit, which is installed in the U-shaped pipe 14; the conveying unit is used to convey the sampler 15 to move in the U-shaped pipe 14.

[0019] As an embodiment of the present invention, a baffle 155 is arranged inside the tube orifice of the sampling tube 152; the baffle 155 is rotatably connected to the inner wall of the sampling tube 152 through a torsion spring.

[0020] As an embodiment of the present invention, three groups of insertion tubes 141 are provided; the three groups of insertion tubes 141 are distributed up and down; During operation, when extracting the strains in the existing culturing stage, a sterile pipette or pipette tube is mostly used to suck a certain amount of culture solution as a sample from the sampling port 23 of a culture container (such as a seed tank); although the sampling operation is usually carried out in a relatively clean environment, and before the sampling operation, the sampling port 23 and its connected pipeline part are also subjected to strict steam sterilization treatment; however, it is inevitable that various miscellaneous bacteria spores may still be suspended in the surrounding air; when the sampling port 23 is opened for sampling, the miscellaneous bacteria suspended in the air will fall into the sampling port 23, thereby causing a risk of bacterial contamination to the strains in the seed tank; In response, the present invention sets a partition plate 13, so that the partition plate 13 divides the interior of the tank body 1 into two independent chambers, enabling the sampler 15 to first enter the chamber above the partition plate 13. Thus, when the miscellaneous bacteria in the outside air enter the chamber above the partition plate 13 through the sampling port 23, the miscellaneous bacteria will be sterilized. Then, the sterilized sampler 15 samples the strains cultured in the chamber below the partition plate 13 through the U-shaped tube 14, thereby preventing the miscellaneous bacteria suspended in the upper air from directly falling into the chamber below the partition plate 13, reducing the risk of bacterial contamination of the strains in the lower chamber; ensuring the safety of strain extraction, and at the same time preventing the extracted strains from being contaminated by the miscellaneous bacteria in the air, reducing the fluctuation range of the detection results and making the detection data more accurate; In the initial state, the two heating pipes 114 on the outer wall of the tank body 1 are connected to the external hot water pipeline, the air inlet 111 and the air outlet 112 on the outer wall of the tank body 1 are connected to the external steam pipeline, a solenoid valve is installed in the discharge port 11 and the solenoid valve is in a closed state, and sealing flanges are installed at one ends of the feed port 21 and the sampling port 23 communicating with the outside; before the user conveys the colony culture solution to the tank body 1, the sealing flange at the feed port 21 needs to be removed, so that the colony culture solution containing colonies enters the chamber below the partition plate 13 through the feed port 21 and the connecting pipe 132; subsequently, control the external heating pipe 114 to convey hot water into the spiral groove 113, so that the hot water can heat the colony culture solution in the tank body 1 through the tank body 1 wall; at the same time, control the driving motor 22 to operate, so that the driving motor 22 can drive the stirring rod 12 to rotate, so that the stirring rod 12 can stir the colony culture solution in the tank body 1, making the temperature and colony concentration of the stirred colony culture solution both in a uniform state; at this time, the temperature data transmitted from the temperature sensor in the tank body 1 to the outside can be used to monitor the colony culture temperature in the tank body 1 in real time to ensure that the colonies in the tank body 1 are always cultured in a suitable temperature environment; Before extracting and detecting the colonies in the tank body 1, the user needs to open the sampling port 23. At this time, the user puts the sampler 15 into the chamber above the partition 13 through the sampling port 23, so that the sampler 15 is placed on the conveying unit. Then, the user controls the external steam pipeline to transport high-temperature steam into the chamber above the partition 13 through the air inlet 111, so that the high-temperature steam can sterilize the sampler 15 on the conveying unit. When the miscellaneous bacteria in the external air enter the chamber above the partition 13 along with the sampler 15, the high-temperature steam transported into the chamber above the partition 13 can sterilize the sampler 15, preventing the miscellaneous bacteria suspended in the air from directly falling into the chamber below the partition 13 during the process of colony extraction, and preventing the bacteria in the chamber below the partition 13 from being contaminated; in addition, an aerogel coating is applied to the upper end of the partition 13, and the aerogel has a nano-porous structure, so that the partition 13 has an extremely low thermal conductivity, enabling the partition 13 to isolate the heat transfer of the high-temperature steam in the upper chamber to the lower chamber. Similarly, the inner wall of the U-shaped tube 14 is also coated with an aerogel coating, reducing the temperature of the high-temperature steam entering the U-shaped tube 14 transferred to the colony culture solution, so as to ensure that the temperature environment for colony culture in the lower chamber remains stable all the time; When it is necessary to extract and detect the colonies in the tank body 1, it is necessary to first control the telescopic unit to drive the sampler 15 into the U-shaped tube 14 until the sampler 15 moves to the intubation tube 141. At this time, control the electromagnetic column 154 to be slowly energized, so that the magnetic force of the electromagnetic column 154 continuously increases. Since the sampling tube 152 is pushed by the connecting spring 153, and when the electromagnetic column 154 starts to be energized, the magnetic force is small. At this time, the electromagnetic column 154 cannot adsorb the sampler 15 to squeeze the connecting spring 153, and at this time, the electromagnetic column 154 generates a magnetic adsorption force on the straight tube 142, so that the straight tube 142 moves in the direction close to the sampling tube 152 under the adsorption of the electromagnetic column 154, so that the distance between the bottom of the straight tube 142 and the intubation tube 141 continuously decreases. Since the straight tube 142 and the intubation tube 141 are in sliding and sealing contact; when the distance between the straight tube 142 and the intubation tube 141 increases, the colony culture solution in the tank body 1 will enter the intubation tube 141 through the mounting hole 143 on the surface of the intubation tube 141. At this time, the colony culture solution is located between the intubation tube 141 and the straight tube 142. At this time, the straight tube 142 extending out of the intubation tube 141 is inserted into the nozzle of the sampling tube 152, so that the straight tube 142 pushes the baffle 155 to insert into the sampling tube 152 against the torsion of the torsion spring. At this time, the straight tube 142 and the nozzle of the sampling tube 152 are in sliding and sealing contact; as the magnetic force of the electromagnetic column 154 continuously increases, the adsorption force of the sampling tube 152 by the magnetic force of the electromagnetic column 154 continuously increases, so that the sampling tube 152 is adsorbed by the electromagnetic column 154 and squeezes the connecting spring 153 to move in the direction close to the straight tube 142; since the sampling tube 152 is adsorbed by four electromagnetic columns 154, and the straight tube 142 is only adsorbed by two electromagnetic columns 154, the electromagnetic adsorption force received by the sampling tube 152 is greater than the electromagnetic adsorption force received by the straight tube 142. When the sampling tube 152 adsorbed by the electromagnetic column 154 squeezes the connecting spring 153, the adsorbed sampling tube 152 can push the straight tube 142 into the intubation tube 141, so that the colony culture solution in the intubation tube 141 is squeezed by the straight tube 142. Since the intubation tube 141 and the end of the straight tube 142 away from the sampling tube 152 are both provided with mounting holes 143, and a one-way valve is installed in the mounting hole 143, the colony culture solution in the intubation tube 141 is blocked by the one-way valve in the mounting hole 143 of the intubation tube 141 and cannot flow into the tank body 1, so that the colony culture solution in the intubation tube 141 can only enter the sampling tube 152 through the mounting hole 143 of the straight tube 142; when the straight tube 142 is completely pushed into the intubation tube 141, control the electromagnetic column 154 to be powered off. At this time, the sampling tube 152 no longer generates a driving force on the straight tube 142. At this time, the sampling tube 152 is pushed by the restoring force of the connecting spring 153 to move away from the straight tube 142, so that the straight tube 142 extends out of the nozzle of the sampling tube 152, and the baffle 155 in the nozzle of the sampling tube 152 is reset under the action of the restoring force of the torsion spring, so that the baffle 155 blocks and seals the nozzle of the sampling tube 152, thereby preventing the colony culture solution in the sampling tube 152 from flowing out of the nozzle;Until the conveying unit drives the sampler 15 through the U-shaped pipe 14 to the sampling port 23, at this time, the user directly takes out the sampler 15 with the extracted colonies and transports it to an external liquid chromatograph, so that the liquid chromatograph can determine whether there are contaminating bacteria by analyzing the components of the microbial metabolites in the extracted colony culture solution; the reason for setting three groups of insertion pipes 141 is to collect the colony culture solutions at different heights of the tank body 1, which can not only analyze the growth conditions of the colonies at different heights. For example, if it is found that the colonies in the upper region of the tank grow too fast but the quality of the metabolites is poor, while the colonies in the lower region grow slowly, then according to this information, fermentation process parameters such as the stirring speed and the heating temperature at different heights of the tank body 1 (heating the tank body 1 by transporting hot water upward from the lower heating pipe 114) can be adjusted accordingly, so that the growth and metabolism of the colonies in the whole tank are more balanced, and the efficiency of fermentation production and the product quality are improved.

[0021] As an implementation manner of the present invention, the conveying unit includes a conveyor belt 16; a tooth groove 161 is formed on the inner ring wall of the conveyor belt 16; a clamping groove 162 is formed on the outer ring wall of the conveyor belt 16; a clamping block 156 is fixedly connected to one side of the sampler 15 close to the clamping groove 162; the clamping block 156 is inserted into the clamping groove 162; a magnet 163 is embedded at the bottom of the clamping groove 162; a straight gear shaft 17 is rotatably connected to the inner wall of the tank body 1; the straight gear shaft 17 is in meshing transmission with the tooth groove 161 of the conveyor belt 16; the straight gear shaft 17 is connected to the output shaft of the driving motor 22 through a connecting unit.

[0022] As an implementation manner of the present invention, the connecting unit includes a mounting frame 171, and the mounting frame 171 is fixedly connected to the inner wall of the tank body 1; the straight gear shaft 17 is rotatably connected to the mounting frame 171; a bevel gear shaft 172 is arranged above the straight gear shaft 17; the bevel gear shaft 172 is connected to the straight gear shaft 17 through a transmission belt 173 for belt transmission connection; a bevel gear ring 174 is slidably connected to the surface of the output shaft of the driving motor 22; the bevel gear ring 174 is connected to the output shaft of the driving motor 22 through a first hydraulic push rod 175; the bevel gear ring 174 is meshed with the bevel gear shaft 172.

[0023] As an implementation manner of the present invention, the connecting unit further includes a connecting rod 221, a rectangular groove 222 is formed at the lower end of the output shaft of the driving motor 22; the connecting rod 221 is slidably and sealingly connected in the rectangular groove 222; a second hydraulic push rod 223 is arranged in the rectangular groove 222; one end of the second hydraulic push rod 223 is fixedly connected to the connecting rod 221, and the other end is fixedly connected to the bottom of the rectangular groove 222; a groove 224 is formed at one end of the stirring rod 12 close to the driving motor 22; the end of the connecting rod 221 away from the rectangular groove 222 is slidably and sealingly connected in the groove 224.

[0024] As an implementation manner of the present invention, the pushing unit includes an electric push rod 24; one end of the electric push rod 24 is fixedly connected to the partition plate 13, and the other end is fixedly connected with a rubber rod 25.

[0025] During operation, when it is necessary to extract and detect the colonies in the tank body 1, first control the electric push rod 24 to operate so that the electric push rod 24 can push the connecting rod 221 into the sampling port 23. Then, the user uses a sterile clamp to hold the sampler 15 into the sampling port 23, so that the sampler 15 is placed on the rubber rod 25. Since the rubber rod 25 is set to be T-shaped, the T-shaped rubber rod 25 can be located between the two clamping blocks 156 of the sampler 15, so that the upper end of the rubber rod 25 is deformed by the extrusion of the clamping blocks 156, and the rubber rod 25 is pressed against the clamping blocks 156 under the action of its own elastic restoring force, increasing the friction force between the rubber block and the clamping blocks 156; Therefore, when the electric push rod 24 contracts, the electric push rod 24 can drive the sampler 15 to descend through the rubber rod 25, preventing the sampler 15 from slipping off the upper end of the rubber rod 25 under the action of its own gravity until the electric push rod 24 drives the sampler 15 to fall onto the conveyor belt 16 through the rubber rod 25, so that the clamping blocks 156 of the sampler 15 are inserted into the card slots 162 of the conveyor belt 16; Since the clamping blocks 156 are made of iron metal material, the magnets 163 in the card slots 162 can generate a magnetic adsorption force on the clamping blocks 156, so that the clamping blocks 156 can be tightly clamped in the card slots 162 under the adsorption of the magnets 163, enabling the sampler 15 and the conveyor belt 16 to be tightly connected; Then, seal the sampling port 23 and control the external steam pipeline to transport steam into the chamber above the partition plate 13 for sterilization treatment of the sampler 15; Since the second hydraulic push rod 223 is in the extended state in the initial state, at this time, the connecting rod 221 is pushed by the second hydraulic push rod 223 to extend out of the rectangular groove 222 and insert into the groove 224 at the upper end of the stirring rod 12. At this time, the driving motor 22 drives the stirring rod 12 to rotate through the connecting rod 221, and the bevel gear ring 174 is pulled by the first hydraulic push rod 175 and moves away from the bevel gear shaft 172. At this time, the bevel gear shaft 172 is separated from the bevel gear ring 174; when it is necessary to extract and detect the colonies in the tank body 1, it is necessary to first control the second hydraulic push rod 223 to contract, so that the second hydraulic push rod 223 pulls the connecting rod 221 into the rectangular groove 222, so that the connecting rod 221 extends out of the groove 224 and separates from the stirring rod 12. At this time, the first hydraulic push rod 175 extends, so that the first hydraulic push rod 175 pushes the bevel gear ring 174 to descend, so that the bevel gear ring 174 meshes with the bevel gear shaft 172. Subsequently, the driving motor 22 is controlled to operate, so that the driving motor 22 drives the bevel gear shaft 172 to rotate through the bevel gear ring 174, so that the bevel gear shaft 172 drives the straight gear shaft 17 connected to it by belt drive to rotate, so that the straight gear shaft 17 can push the conveyor belt 16 to slide in the U-shaped pipe 14 through the tooth grooves 161 of the conveyor belt 16 meshed with it, so that the conveyor belt 16 can drive the sampler 15 clamped in the card slot 162 to move into the U-shaped pipe 14 synchronously, so that the conveyor belt 16 can drive the sampler 15 to the insertion pipe 141 in the U-shaped pipe 14, thereby realizing the colony extraction operation; until the colonies are completely extracted, the conveyor belt 16 drives the sampler 15 to extend out from the other end of the U-shaped pipe 14 and below the sampling port 23. At this time, the electric push rod 24 is controlled to extend, so that the electric push rod 24 pushes the sampler 15 into the sampling port 23 through the rubber rod 25, which is convenient for the operator to take out the sampler 15, effectively improving the practicability of the present invention.

[0026] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the descriptions in the specification only illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for extracting and detecting microbial fermentation strains of fermented beverages, comprising: A tank body (1), wherein a tank cover (2) is fixedly mounted on the upper end of the tank body (1); a feed port (21) communicating with the tank body (1) is provided on the upper end of the tank cover (2); a discharge port (11) is provided on the lower end of the tank body (1); a drive motor (22) is fixedly mounted on the upper end of the tank cover (2); a stirring rod (12) is rotatably connected inside the tank body (1); the drive motor (22) is used to drive the stirring rod (12) to rotate; a temperature sensor is embedded on the inner wall of the tank body (1); and the characteristics are: A partition (13), the partition (13) being fixedly connected to the inner wall of the tank body (1); the partition (13) divides the interior of the tank body (1) into two independent chambers; a through groove (131) is provided at the upper end of the partition (13); the stirring rod (12) is rotatably connected to the partition (13); the through groove (131) is connected to the feed port (21) through a connecting pipe (132); a sampling port (23) is provided on the surface of the tank cover (2); a U-shaped tube (14) is provided below the partition (13); both ends of the U-shaped tube (14) pass through the partition (13); an air inlet (111) and an air outlet (112) are provided on the surface of the tank body (1); a spiral groove (113) is provided inside the tank body (1); a heating tube (114) connected to the spiral groove (113) is provided on the outer surface of the tank body (1); A sampling module is installed in the U-shaped tube (14); the sampling module is used to sample the bacterial species in the tank (1).

2. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 1, characterized in that: The sampling module comprises: A cannula (141), the cannula (141) is embedded in the outer wall of the U-shaped tube (14); one end of the cannula (141) close to the sampler (15) is slidably and sealingly connected to a straight tube (142); side walls of the cannula (141) and the straight tube (142) are both provided with mounting holes (143); a one-way valve is fixedly installed in the mounting hole (143); A sampler (15), wherein a mounting groove (151) is provided on the surface of the sampler (15); a sampling tube (152) is slidably and sealedly connected in the mounting groove (151); the sampling tube (152) and the sampler (15) are connected via a connecting spring (153); an electromagnetic column (154) is embedded in the sampler (15); and the number of the electromagnetic columns (154) is four; A conveying unit is installed in the U-shaped tube (14); the conveying unit is used to convey the sampler (15) to move in the U-shaped tube (14).

3. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 2, characterized in that: A blocking piece (155) is arranged in the tube mouth of the sampling tube (152); the blocking piece (155) is rotatably connected to the inner wall of the sampling tube (152) via a torsion spring.

4. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 3, characterized in that: The insert tubes (141) are provided in three groups; the three groups of insert tubes (141) are distributed up and down.

5. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 2, characterized in that: The conveying unit comprises a conveyor belt (16); the inner ring wall of the conveyor belt (16) is provided with a tooth groove (161); the outer ring wall of the conveyor belt (16) is provided with a clamping groove (162); a clamping block (156) is fixedly connected to a side of the sampler (15) close to the clamping groove (162); the clamping block (156) is inserted into the clamping groove (162); a magnet (163) is embedded in the bottom of the clamping groove (162); a spur gear shaft (17) is rotatably connected to the inner wall of the tank body (1); the spur gear shaft (17) and the tooth groove (161) of the conveyor belt (16) are meshed for transmission; the spur gear shaft (17) is connected to the output shaft of the driving motor (22) via a connecting unit.

6. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 5, characterized in that: The connection unit comprises a mounting frame (171), wherein the mounting frame (171) is fixedly connected to the inner wall of the tank body (1); the spur gear shaft (17) is rotatably connected to the mounting frame (171); a bevel gear shaft (172) is arranged above the spur gear shaft (17); the bevel gear shaft (172) and the spur gear shaft (17) are connected by belt transmission via a transmission belt (173); a bevel gear ring (174) is slidably connected to the surface of the output shaft of the drive motor (22); the bevel gear ring (174) is connected to the output shaft of the drive motor (22) via a No. 1 hydraulic push rod (175); and the bevel gear ring (174) is meshed with the bevel gear shaft (172).

7. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 6, characterized in that: The connection unit also includes a connecting rod (221); a rectangular groove (222) is provided at the lower end of the output shaft of the driving motor (22); the connecting rod (221) is slidably sealed and connected in the rectangular groove (222); a second hydraulic push rod (223) is provided in the rectangular groove (222); one end of the second hydraulic push rod (223) is fixedly connected to the connecting rod (221), and the other end is fixedly connected to the bottom of the rectangular groove (222); a groove (224) is provided at one end of the stirring rod (12) close to the driving motor (22); and one end of the connecting rod (221) away from the rectangular groove (222) is slidably sealed and connected in the groove (224).

8. The device for extracting and detecting microbial fermentation strains of fermented beverages according to claim 7, characterized in that: The pushing unit comprises an electric push rod (24); one end of the electric push rod (24) is fixedly connected to the partition (13), and the other end is fixedly connected to a rubber rod (25).

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