Real-time dynamic monitoring and analyzing system for wound healing process

Abstract

The invention discloses a real-time dynamic monitoring and analyzing system for a wound healing process, and relates to the technical field of monitoring of wound healing in orthopaedic surgery. The current wound healing monitoring stage is monitored according to the wound area change, monitoring time, cell survival rate change, culture solution adjustment and temperature in a culture box in the wound healing process, the wound healing rate YH, the culture solution adjustment amount PY and the temperature adjustment amount HW are sequentially calculated and output through a monitoring adjustment module, and the central control module is used for controlling the wound healing rate YH, the culture solution adjustment amount PY and the temperature adjustment amount HW. The monitoring transmission module is used for transmitting a culture solution adjusting instruction to the culture solution adjusting module, and the central control module is used for transmitting a temperature adjusting instruction in the culture box to the temperature adjusting module through the monitoring transmission module, so that real-time monitoring and analysis considering nonlinear characteristics are realized, and a strategy is dynamically adjusted; and a closed-loop feedback mechanism is formed, so that the flexibility and the accuracy of dynamic monitoring of the system are improved.

Description

Technical Field

[0001] The present invention relates to the technical field of monitoring plastic surgery wound healing, and specifically to a real-time dynamic monitoring and analysis system for the wound healing process. Background Technique

[0002] Wound healing refers to the process of recovery after the body is subjected to external forces and tissues such as the skin are severed or damaged, including the regeneration of various tissues and the complex combination of granulation tissue hyperplasia and scar tissue formation, showing the synergistic effect of various processes. The basic process of wound healing: acute inflammation phase → cell proliferation phase → scar formation phase → epidermal and other tissue regeneration. And according to the degree of injury and the presence or absence of infection, wound healing can be divided into three types: primary healing, secondary healing, and tertiary healing. In the field of plastic surgery, wound healing is a complex and crucial process, and it is affected by various factors, including wound size, cell survival rate, nutrient supply, and environmental factors.

[0003] Currently, in the existing dynamic monitoring and analysis system for wound healing, during the process of cell movement at the wound, generally, the internal environment of the culture dish cannot be adjusted, which leads to the death of some cells, thus resulting in poor experimental effects. Moreover, there are no objective and quantitative monitoring indicators. In addition, the existing adjustment strategies are often based on historical data and empirical formulas, and it is difficult to respond to the changes in the wound healing process in a timely and accurate manner, nor can the culture medium and incubator conditions be dynamically adjusted according to the real-time monitoring results. Summary of the Invention

[0004] The purpose of the present invention is to provide a real-time dynamic monitoring and analysis system for the wound healing process, which solves the problems raised in the above background technique.

[0005] To achieve the above purpose, the present invention provides the following technical solutions, including a central control module, a monitoring and transmission module, a wound regular collection module, a monitoring and adjustment module, a culture medium adjustment module, and a temperature adjustment module;

[0006] The specific implementation steps are as follows:

[0007] Step 1: Use the wound regular collection module to monitor the wound area change, monitoring time, cell survival rate change, culture medium adjustment, and temperature in the incubator during the current wound healing monitoring stage of the wound healing process, and transmit them to the monitoring and adjustment module and the central control module respectively through the monitoring and transmission module;

[0008] Step 2: Use the monitoring and adjustment module to calculate and output the wound healing rate YH, the culture medium adjustment amount PY, and the temperature adjustment amount HW in sequence, and transmit them to the central control module through the monitoring and transmission module;

[0009] Step 3: Use the central control module and transmit the adjustment instruction of the culture medium to the culture medium adjustment module through the monitoring and transmission module for real-time dynamic adjustment;

[0010] Step 4: Use the central control module and transmit the instruction for adjusting the temperature in the incubator to the temperature adjustment module through the monitoring and transmission module for real-time dynamic adjustment;

[0011] Among them, the monitoring and adjustment module includes a unit for understanding the progress of wound healing, a unit for guiding the adjustment of the culture medium, and a unit for guiding the adjustment of temperature.

[0012] Optionally, the central control module is sequentially connected to the monitoring and transmission module, the wound regular collection module, the monitoring and adjustment module, the culture medium adjustment module, and the temperature adjustment module, and the wound regular collection module, the monitoring and adjustment module, the culture medium adjustment module, and the temperature adjustment module are all connected to the monitoring and transmission module and transmit signals to the central control module through the monitoring and transmission module.

[0013] Optionally, the calculation formula of the unit for understanding the progress of wound healing is as follows:

[0014]

[0015] Among them:

[0016] YH is the wound healing rate;

[0017] M b is the wound area at the second moment, and M b reflects the size of the wound area at the end moment of the current wound healing monitoring stage;

[0018] M a is the wound area at the first moment, and M a reflects the size of the wound area at the middle moment of the current wound healing monitoring stage;

[0019] t b is the second moment point, and t b reflects the monitoring moment at the end of the current wound healing monitoring stage;

[0020] t a is the first moment point, reflecting the monitoring moment at the middle of the current wound healing monitoring stage;

[0021] XB is the current cell survival rate;

[0022] XB opt is the expected cell survival rate, and XB optReflect the average degree of the current cell survival rate XB when the wound healing rate YH shows an upward trend during consecutive wound healing monitoring phases;

[0023] Is the square root of the proportion of the wound area reduction per unit time and reflects the non-linear characteristics of the healing process.

[0024] Optionally, the expected cell survival rate XB opt According to the three stages of primary intention healing, secondary intention healing, and tertiary intention healing of the set wound healing, regularly and separately collect the current cell survival rate XB showing an upward trend in the three stages, and perform separate averaging calculations on the current cell survival rate XB collected in the three stages. The calculation formula for the averaging calculation of the current cell survival rate XB is as follows:

[0025]

[0026] Where:

[0027] N is the collection amount;

[0028] XB i Is the i-th cell survival rate;

[0029] Collect the current cell survival rate XB in the three stages and calculate to obtain the expected cell survival rate XB opt The calculation formulas are the same.

[0030] Optionally, the calculation formula of the guiding culture solution adjustment unit is as follows:

[0031]

[0032] Where:

[0033] PY is the culture solution adjustment amount;

[0034] If PY is a positive value, it means that the volume of the culture solution needs to be increased;

[0035] If PY is a negative value, it means that the volume of the culture solution needs to be decreased;

[0036] If PY is 0, it means that the volume of the culture solution does not need to be adjusted;

[0037] KT is the culture solution adjustment coefficient, and KT determines the adjustment ratio according to the wound healing rate YH:

[0038] If the wound healing rate YH compared with the previous wound healing rate YH old Increases, it means that the wound heals quickly and the amount of the culture solution needs to be reduced to avoid waste. The calculation formula for the culture solution adjustment coefficient K is:

[0039] If the wound healing rate YH is lower than the previous wound healing rate YH old it indicates that the wound healing is slow and more nutritional support is needed. The calculation formula for the culture medium adjustment coefficient K is as follows:

[0040] YH old is the previous wound healing rate, reflecting the result of the wound healing rate YH calculated and output during the previous wound healing monitoring stage.

[0041] Optionally, the calculation formula of the guiding temperature adjustment unit is as follows:

[0042]

[0043] Where:

[0044] HW is the temperature adjustment amount, which reflects the amount by which the temperature in the incubator needs to be adjusted;

[0045] If HW is a positive value, it means that the temperature in the incubator needs to be increased;

[0046] If HW is a negative value, it means that the temperature in the incubator needs to be decreased;

[0047] If HW is 0, it means that the temperature in the incubator does not need to be adjusted;

[0048] PY old is the previous culture medium adjustment amount, reflecting the result of the culture medium adjustment amount PY calculated and output during the previous wound healing monitoring stage;

[0049] W opt is the expected culture temperature;

[0050] W is the current temperature;

[0051] W max is the maximum temperature adjustment value;

[0052] W min is the minimum temperature adjustment value;

[0053] [(PY - PY old ) × (W opt - W)] calculates the adjustment amount of the change in culture medium demand and the temperature difference, and this adjustment amount reflects the demand for incubator temperature adjustment caused by the change in culture medium demand.

[0054] Optionally, the adjustment instruction of the culture medium transmitted by the central control module to the culture medium adjustment module through the monitoring and transmission module is the result of the culture medium adjustment amount PY;

[0055] The instruction for temperature adjustment in the incubator transmitted by the central control module to the temperature adjustment module through the monitoring and transmission module is the result of the temperature adjustment amount HW.

[0056] Optionally, the expected culture temperature W opt is the same as the setting and calculation of the expected cell survival rate XB opt and is calculated in the same way. According to the three stages of primary healing, secondary healing, and tertiary healing of wound healing set, the current temperature W showing an upward trend in the three stages is regularly and separately collected, and the current temperature W collected in the three stages is separately averaged. The calculation formula for the averaging of the current temperature W is as follows:

[0057]

[0058] Where:

[0059] N is the collection amount;

[0060] W i is the i-th temperature;

[0061] And the current temperature W is collected in the three stages and the calculated expected culture temperature W opt has the same calculation formula.

[0062] Optionally, the devices used by the central control module include a control system;

[0063] The devices used by the monitoring and transmission module include data transmission devices;

[0064] The devices used by the wound regular collection module include high-precision measurement devices, image processing software, and cell survival rate detection devices;

[0065] The devices used by the monitoring and adjustment module include computer devices;

[0066] The devices used by the culture medium adjustment module include culture medium adjustment devices;

[0067] The devices used by the temperature adjustment module include incubator temperature adjustment devices.

[0068] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0069] First, by measuring the change in wound area over time and combining with the current cell survival rate XB, the wound healing rate YH is calculated by the wound healing progress understanding unit, thereby reflecting the non-linear characteristics of wound healing in the current wound healing monitoring stage. The culture medium adjustment guiding unit then adjusts the culture medium according to the wound healing rate YH and combines with the expected cell survival rate XB opt, the required amount of culture fluid adjustment PY is calculated to meet the needs of the wound for flexible adjustment of nutrition and support. Finally, the guiding temperature adjustment unit is based on the change of the culture fluid adjustment amount PY and the expected culture temperature W opt As well as the actual temperature in the current incubator, combined with the wound healing rate YH and the limit value of the temperature adjustment, the temperature adjustment amount HW is calculated to optimize the wound healing environment. At this point, the system forms a closed-loop feedback mechanism, and through real-time monitoring, analysis and adjustment, it continuously optimizes the wound healing environment, improves the wound healing rate YH and cell survival rate.

[0070] Second, the present invention monitors and analyzes the changing trend of the current temperature W and the current cell survival rate XB in real time, and combines the rising trend of the wound healing rate YH to enable the system to dynamically adjust the expected culture temperature W opt The expected cell survival rate XB opt values ​​to better guide the adjustment of culture medium and temperature, and optimize the wound healing environment. This innovative approach not only improves the flexibility and accuracy of the system, but also provides new ideas and methods for monitoring and analyzing the wound healing process in plastic surgery. BRIEF DESCRIPTION OF THE DRAWINGS

[0071] Figure 1 This is a method flow chart of the real-time dynamic monitoring and analysis system for the wound healing process;

[0072] Figure 2 This is a schematic diagram of the overall structure of the real-time dynamic monitoring and analysis system for the wound healing process;

[0073] Figure 3 It is a structural schematic diagram of the monitoring and adjustment module in the present invention. DETAILED DESCRIPTION

[0074] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0075] The present real-time dynamic monitoring and analysis system for the wound healing process is different from the existing real-time dynamic monitoring and analysis system for the wound healing process. The existing real-time dynamic monitoring and analysis system for the wound healing process has the problems of single monitoring means, lagging adjustment strategy, and lack of closed-loop feedback mechanism. The present algorithm unit realizes real-time monitoring and analysis considering nonlinear characteristics, and dynamically adjusts the strategy, thereby forming a closed-loop feedback mechanism, thereby improving the flexibility and accuracy of the system's dynamic monitoring.

[0076] Example 1, please refer to Figures 1 to 3 , this example provides a real-time dynamic monitoring and analysis system for the wound healing process, including a central control module, a monitoring and transmission module, a wound regular collection module, a monitoring and adjustment module, a culture medium adjustment module, and a temperature adjustment module;

[0077] The specific implementation steps are as follows:

[0078] Step 1: Use the wound regular collection module to monitor the wound area change, monitoring time, cell survival rate change, culture medium adjustment, and temperature in the incubator during the current wound healing monitoring stage, and transmit them to the monitoring and adjustment module and the central control module respectively through the monitoring and transmission module;

[0079] Step 2: Use the monitoring and adjustment module to calculate and output the wound healing rate YH, the culture medium adjustment amount PY, and the temperature adjustment amount HW in sequence, and transmit them to the central control module through the monitoring and transmission module;

[0080] Step 3: Use the central control module and transmit the culture medium adjustment instruction to the culture medium adjustment module through the monitoring and transmission module for real-time dynamic adjustment;

[0081] Step 4: Use the central control module and transmit the instruction for adjusting the temperature in the incubator to the temperature adjustment module through the monitoring and transmission module for real-time dynamic adjustment;

[0082] Among them, the monitoring and adjustment module includes a unit for understanding the wound healing progress, a unit for guiding the culture medium adjustment, and a unit for guiding the temperature adjustment;

[0083] The central control module is sequentially connected to the monitoring and transmission module, the wound regular collection module, the monitoring and adjustment module, the culture medium adjustment module, and the temperature adjustment module, and the wound regular collection module, the monitoring and adjustment module, the culture medium adjustment module, and the temperature adjustment module are all connected to the monitoring and transmission module, and transmit signals to the central control module through the monitoring and transmission module;

[0084] The equipment used by the central control module includes a control system;

[0085] The equipment used by the monitoring and transmission module includes data transmission equipment;

[0086] The equipment used by the wound regular collection module includes high-precision measurement equipment, image processing software, and cell survival rate detection equipment;

[0087] The equipment used by the monitoring and adjustment module includes computer equipment;

[0088] The equipment used by the culture medium adjustment module includes culture medium adjustment equipment;

[0089] The devices used in the temperature adjustment module include the incubator temperature adjustment device.

[0090] In this embodiment, the system forms a closed-loop system through the mutual cooperation of three algorithm units, combined with the three calculation results of the wound healing rate YH, the culture medium adjustment amount PY, and the temperature adjustment amount HW. Moreover, the calculation results of the culture medium adjustment amount PY and the temperature adjustment amount HW can also affect the calculation of the feedback to the wound healing rate YH. Thus, by real-time monitoring and analyzing the wound healing process, the culture medium and the incubator environment are dynamically adjusted to optimize the wound healing effect.

[0091] Please refer to Figures 1 to 3 , and the calculation formula of the wound healing progress unit is as follows:

[0092]

[0093] Where:

[0094] YH is the wound healing rate;

[0095] M b is the wound area at the second moment, and M b reflects the size of the wound area at the end moment of the current wound healing monitoring stage;

[0096] M a is the wound area at the first moment, and M a reflects the size of the wound area at the middle moment of the current wound healing monitoring stage;

[0097] t b is the second moment point, and t b reflects the monitoring moment at the end of the current wound healing monitoring stage;

[0098] t a is the first moment point, reflecting the monitoring moment in the middle of the current wound healing monitoring stage;

[0099] XB is the current cell survival rate;

[0100] XB opt is the expected cell survival rate, and XB opt reflects the average degree of the current cell survival rate XB when the wound healing rate YH shows an upward trend in consecutive wound healing monitoring stages;

[0101] is the square root of the proportion of the wound area reduction per unit time, and reflects the non-linear characteristics of the healing process.

[0102] In this embodiment: First, in this algorithm unit The calculation part calculates the relative change rate of the wound area between two measurements, that is, the amount by which the wound area decreases per unit time. Here, a simple division operation is used because the decrease in the wound area is proportional to the time interval, which reflects the basic trend of wound healing. This change rate serves as the basis for calculating the wound healing rate YH and can intuitively reflect the speed of wound healing. Since the wound healing process often has non-linear characteristics, that is, the healing speed is faster in the initial stage and then gradually slows down, a square root operation is used to adjust the wound area change rate to better reflect this non-linear change and avoid extreme results caused by too large a wound area change rate, thereby improving the accuracy and reliability of the calculation;

[0103] The calculation part is one of the important factors affecting wound healing. When the cell survival rate calculated by this calculation part is high, the wound healing speed is usually fast. Therefore, this part calculates the ratio between the current cell survival rate XB and the expected cell survival rate XB opt to reflect the impact of the cell survival rate on the wound healing rate. Using this ratio as an adjustment factor for calculating the wound healing rate YH can make the wound healing rate YH more accurately reflect the actual state of wound healing;

[0104] As the core parameter of the formula, the change rate of the wound area in this algorithm unit directly reflects the wound healing speed. By continuously monitoring the wound area and calculating its change rate, the progress of wound healing can be intuitively understood. The introduction of the square root operation in it enables the formula to more realistically reflect the non-linear characteristics of wound healing;

[0105] The wound healing rate YH value calculated by the unit for understanding the progress of wound healing is not only a quantitative index but also an important basis for subsequent culture medium adjustment. When the wound healing rate YH value is low, it means that wound healing is blocked. At this time, it is necessary to increase nutritional support and adjust the type, concentration, and volume of the culture medium. When the wound healing rate YH value is high, the nutritional supply is appropriately reduced to avoid waste;

[0106] It should be noted that selecting the wound area M at the first moment in the middle section a can capture a key node in the wound healing process. The wound area data at this node can reflect the wound healing speed, trend, and existing problems under specific treatment and care conditions. Selecting the wound area M at the second moment at the end b, it can reflect the final healing effect of this stage. This data point not only helps to understand the recovery of the wound after a complete treatment cycle but also serves as an important basis for evaluating the treatment effect. Selecting the measurement time points as the midpoint and the end point can effectively avoid collisions with the end point of the previous stage or the start point of the next stage. The advantage of doing this is that it ensures the continuity of observation and analysis, enabling the system to monitor the wound healing process within a complete and interference-free time period. This continuity is crucial for accurately evaluating the treatment effect and promptly discovering and solving problems.

[0107] Please refer to Figures 1 to 3 , and the calculation formula for guiding the culture medium adjustment unit is as follows:

[0108]

[0109] Where:

[0110] PY is the amount of culture medium adjustment;

[0111] If PY is a positive value, it means that the volume of the culture medium needs to be increased;

[0112] If PY is a negative value, it means that the volume of the culture medium needs to be decreased;

[0113] If PY is 0, it means that the volume of the culture medium does not need to be adjusted;

[0114] KT is the culture medium adjustment coefficient, and KT determines the adjustment ratio according to the wound healing rate YH:

[0115] If the wound healing rate YH is larger than the previous wound healing rate YH old , it indicates that the wound heals quickly and the amount of culture medium needs to be reduced to avoid waste. The calculation formula for the culture medium adjustment coefficient K is:

[0116] If the wound healing rate YH is smaller than the previous wound healing rate YH old , it indicates that the wound heals slowly and more nutritional support is needed. The calculation formula for the culture medium adjustment coefficient K is:

[0117] YH old is the previous wound healing rate, which reflects the result of the wound healing rate YH calculated and output in the previous wound healing monitoring stage.

[0118] In this embodiment, first in the YH×KT calculation part, the wound healing rate YH reflects the healing state of the wound, and the culture medium adjustment coefficient KT is an adjustment coefficient determined according to the wound healing rate YH. When the wound healing rate YH is high, it indicates that the wound heals quickly, and at this time, the culture medium needs to be reduced to avoid excessive wetness. On the contrary, when the wound healing rate YH is low, the culture medium needs to be increased to promote wound healing. Therefore, this part calculates the adjustment amount based on the wound healing rate by multiplying the wound healing rate YH by the culture medium adjustment coefficient KT;

[0119] The calculation part is an important factor affecting the demand for the culture medium. When the wound is large and the cell survival rate is low, more culture medium is needed to provide sufficient nutrition and support. On the contrary, the culture medium needs to be reduced. Therefore, this part reflects the influence of these two factors on the demand for the culture medium by calculating the product of the wound size and the ratio of the current cell survival rate XB to the expected cell survival rate XB opt This adjustment amount, as another important component in the calculation of the culture medium adjustment amount PY, can make the adjustment of the culture medium more accurate and personalized;

[0120] This algorithm jointly determines the adjustment amount of the culture medium by guiding the parameters in the culture medium adjustment unit. Since the wound healing states and cell survival rates of each patient are different, these parameters can reflect individual differences and provide a personalized culture medium adjustment strategy for each patient;

[0121] By accurately calculating the adjustment amount of the culture medium, the culture medium adjustment unit guided can ensure that the wound obtains sufficient nutritional support while avoiding unnecessary waste. This optimized nutritional supply strategy helps to promote cell proliferation and differentiation, accelerate wound healing, and the personalized culture medium adjustment strategy and optimized nutritional supply together improve the efficiency of wound healing. By reducing nutritional waste and accelerating wound healing, the culture medium adjustment unit guided helps to shorten the recovery time of patients and relieve pain.

[0122] Please refer to Figures 1 to 3 , the calculation formula of the temperature adjustment unit guided is as follows:

[0123]

[0124] Where:

[0125] HW is the temperature adjustment amount, and HW reflects the amount by which the temperature in the incubator needs to be adjusted;

[0126] If HW is a positive value, it means that the temperature in the incubator needs to be increased;

[0127] If HW is a negative value, it means that the temperature in the incubator needs to be decreased;

[0128] If HW is 0, it means that there is no need to adjust the temperature in the incubator;

[0129] PY old Is the adjustment amount for the previous culture medium, reflecting the result of the adjustment amount PY of the culture medium calculated and output in the previous wound healing monitoring stage;

[0130] W opt Is the expected culture temperature;

[0131] W is the current temperature;

[0132] W max Is the maximum temperature adjustment value;

[0133] W min Is the minimum temperature adjustment value;

[0134] [(PY - PY old ) × (W opt - W)] calculates the adjustment amount of the change in culture medium demand and temperature difference, and this adjustment amount reflects the temperature adjustment demand of the incubator caused by the change in culture medium demand.

[0135] In this embodiment, the calculation of (PY - PY old ) in this algorithm unit first reflects the change in culture medium demand, that is, the difference in the amount of culture medium adjustment between this time and the previous time, and this difference can reflect whether the demand for culture medium by the wound is increasing or decreasing. The calculation of (W opt - W) is the difference between the set expected culture temperature W opt and the current temperature W, which further reflects the gap between the current temperature of the incubator and the ideal temperature. Multiplying these two parts can obtain an adjustment amount based on the change in culture medium demand and temperature difference. This adjustment amount reflects the temperature adjustment demand of the incubator caused by the change in culture medium demand. And multiplying by the calculation part of (YH × W max ) can obtain a temperature adjustment amount based on the wound healing rate YH. This adjustment amount reflects the temperature adjustment demand of the incubator caused by the wound healing state. Then subtracting the minimum temperature adjustment value W min is to ensure that the temperature adjustment amount will not be lower than a minimum limit value. In other words, even if the temperature adjustment amount calculated based on the change in culture medium demand and wound healing rate is very small, it will not cause the temperature of the incubator to drop too much, because too low a temperature will have an adverse effect on wound healing. Therefore, subtracting the minimum temperature adjustment value W min has the calculation significance of protecting the wound, ensuring the rationality of the adjustment amount, and maintaining system stability;

[0136] In this algorithm unit, by adjusting the temperature of the incubator, the guided temperature adjustment unit can create an environment that is more conducive to wound healing. An appropriate culture temperature helps to promote cell metabolism and repair, accelerating the wound healing process. An appropriate culture temperature can not only increase the rate of wound healing, but also reduce the risk of infection and other complications. By optimizing the culture environment, the guided temperature adjustment unit helps to improve the overall treatment effect and relieve the pain of patients;

[0137] The guided temperature adjustment unit, the unit for understanding the progress of wound healing, and the guided culture solution adjustment unit together constitute a closed-loop system. By real-time monitoring of the wound healing rate TH, the culture solution adjustment amount PY, and the temperature adjustment amount HW parameters, and dynamically adjusting according to the changes of these parameters, the system can continuously optimize the wound healing process and improve the treatment effect.

[0138] Please refer to Figures 1 to 3 , the adjustment instruction of the culture solution transmitted by the central control module to the culture solution adjustment module through the monitoring transmission module is the result of the culture solution adjustment amount PY;

[0139] The instruction for adjusting the temperature in the incubator transmitted by the central control module to the temperature adjustment module through the monitoring transmission module is the result of the temperature adjustment amount HW.

[0140] In this embodiment, on the one hand, when the temperature of the incubator is optimized, the cell survival rate will increase, and the wound healing rate will also accelerate. This change will be captured by the unit for understanding the progress of wound healing and used as the basis for the next culture solution adjustment. This dynamic adjustment and feedback mechanism enables the system to make real-time adjustments according to the actual situation of wound healing and optimize the treatment effect;

[0141] On the other hand, through the cyclic influence of the guided temperature adjustment unit on the unit for understanding the progress of wound healing, the system can continuously optimize the wound healing process. At different stages of wound healing, the system will adjust the culture solution and incubator conditions according to the actual situation to ensure that the wound obtains the best nutritional support and environmental conditions. This continuous optimization strategy helps to improve the overall treatment effect and reduce the pain and recovery time of patients;

[0142] In summary, the unit for understanding the progress of wound healing, the guided culture solution adjustment unit, and the guided temperature adjustment unit each play an important role in the real-time dynamic monitoring and analysis system for orthopedic wound healing. They together constitute a closed-loop system. Through real-time monitoring and dynamic adjustment, they continuously optimize the wound healing process and improve the treatment effect. At the same time, the parameters in these formulas also provide profound insights and personalized adjustment strategies, which in turn help to achieve more precise and efficient wound healing treatment.

[0143] Embodiment 2, please refer to Figures 1 to 3, the expected cell survival rate XB opt According to the three stages of primary intention healing, secondary intention healing, and tertiary intention healing of wound healing set, regularly and separately collect the current cell survival rates XB showing an upward trend in the three stages, and perform separate averaging calculations on the current cell survival rates XB collected in the three stages. The calculation formula for the averaging calculation of the current cell survival rate XB is as follows:

[0144]

[0145] Where:

[0146] N is the collection amount;

[0147] XB i is the i-th cell survival rate;

[0148] Collect the current cell survival rate XB in the three stages and calculate to obtain the expected cell survival rate XB opt The calculation formulas are the same;

[0149] The expected culture temperature W opt is the same as the setting and calculation of the expected cell survival rate XB opt That is, according to the three stages of primary intention healing, secondary intention healing, and tertiary intention healing of wound healing set, regularly and separately collect the current temperature W showing an upward trend in the three stages, and perform separate averaging calculations on the current temperature W collected in the three stages. The calculation formula for the averaging calculation of the current temperature W is as follows:

[0150]

[0151] Where:

[0152] N is the collection amount;

[0153] W i is the i-th temperature;

[0154] And collect the current temperature W in the three stages and calculate to obtain the expected culture temperature W opt The calculation formulas are the same.

[0155] In this embodiment, on the one hand, when the wound healing rate YH rises linearly, this usually means that the current cell survival rate XB is within a range conducive to wound healing. At this stage, by observing and analyzing the relationship between the healing rate and the cell survival rate, an expected cell survival rate XB can be determined opt, this expected value reflects that under the current conditions, when the cell survival rate reaches a certain specific level, the wound healing effect is optimal, which can accelerate the wound healing process, reduce the healing time, and the appropriate cell survival rate helps to reduce the risk of infection and other complications, thereby improving the overall treatment effect. However, the optimal cell survival rate varies among different patients. By observing the healing rate and the change of cell survival rate of individual patients, a more personalized treatment plan can be formulated;

[0156] On the other hand, when the wound healing rate YH rises linearly, the current temperature W is also within a range conducive to wound healing. At this stage, the change of the healing rate can be observed by adjusting the incubator temperature, so as to determine a set expected incubation temperature W. opt , thus the adjusted appropriate incubation temperature can promote the normal metabolism and growth of cells, thereby increasing the wound healing rate. And by setting a stable expected incubation temperature, the adverse effects of temperature fluctuations on the cell survival rate and the wound healing rate YH can be reduced. The stable incubation temperature helps to improve the stability and predictability of the treatment, thereby reducing the risk of treatment failure;

[0157] In summary, these three formulas form a closed-loop system. By real-time monitoring and analyzing the wound healing process and dynamically adjusting the culture medium and the incubator environment, when the wound healing rate rises linearly, the expected cell survival rate XB is determined according to the average degree of the current cell survival rate XB and the current temperature W. opt and the expected incubation temperature W opt , the treatment effect can be further optimized. This dynamic adjustment and optimization strategy helps to improve the personalization, stability and efficiency of the treatment, thereby providing a better treatment effect and a faster recovery process.

[0158] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A real-time dynamic monitoring and analysis system for wound healing process, characterized in that: It includes a central control module, a monitoring and transmission module, a wound regular collection module, a monitoring and adjustment module, a culture fluid adjustment module and a temperature adjustment module; The specific implementation steps are as follows: Step 1: Using the wound periodic collection module, the wound area change, monitoring time, cell survival rate change, culture fluid adjustment and temperature in the incubator during the wound healing process are monitored at the current wound healing monitoring stage, and transmitted to the monitoring adjustment module and the central control module respectively through the monitoring transmission module; Step 2: using the monitoring and adjustment module, sequentially calculate and output the wound healing rate YH, the culture fluid adjustment amount PY, and the temperature adjustment amount HW, and transmit them to the central control module through the monitoring transmission module; Step 3: using the central control module and transmitting the adjustment instruction of the culture solution to the culture solution adjustment module through the monitoring transmission module, so as to perform real-time dynamic adjustment; Step 4: using the central control module and transmitting the temperature adjustment instruction in the incubator to the temperature adjustment module through the monitoring transmission module, so as to perform real-time dynamic adjustment; The monitoring and adjusting module includes a wound healing progress understanding unit, a culture fluid adjustment guiding unit and a temperature adjustment guiding unit.

2. A real-time dynamic monitoring and analysis system for wound healing process according to claim 1, characterized in that: The central control module is connected to the monitoring and transmission module, the wound periodic collection module, the monitoring and adjustment module, the culture fluid adjustment module and the temperature adjustment module in sequence, and the wound periodic collection module, the monitoring and adjustment module, the culture fluid adjustment module and the temperature adjustment module are all connected to the monitoring and transmission module, and transmit signals to the central control module through the monitoring and transmission module.

3. A real-time dynamic monitoring and analysis system for wound healing process according to claim 2, characterized in that: The calculation formula for understanding the wound healing progress unit is as follows: in: YH is the wound healing rate; M b is the wound area at the second moment, M b Reflects the wound area size at the end of the current wound healing monitoring stage; M a is the wound area at the first moment, M a Reflects the size of the wound area at the middle moment of the current wound healing monitoring stage; t b is the second time point, t b Reflects the monitoring moment at the end of the current wound healing monitoring stage; t a is the first moment, reflecting the monitoring moment in the middle of the current wound healing monitoring stage; XB is the current cell survival rate; XB opt is the expected rate of cell survival, XB opt It reflects the average degree of current cell survival rate XB when the wound healing rate YH shows an upward trend in the continuous wound healing monitoring stage; It is the square root of the reduction ratio of wound area per unit time and reflects the nonlinear characteristics of the healing process.

4. A real-time dynamic monitoring and analysis system for wound healing process according to claim 3, characterized in that: The expected cell survival rate XB opt According to the three stages of wound healing set as primary healing, secondary healing, and tertiary healing, the current cell survival rates XB showing an upward trend in the three stages are collected regularly and separately, and the current cell survival rates XB collected in the three stages are averaged and calculated respectively. The calculation formula for the average calculation of the current cell survival rate XB is as follows: in: N is the collection volume; XB i is the survival rate of the ith cell; The current cell survival rate XB is collected in three stages and the expected cell survival rate XB is calculated opt The calculation formula is the same.

5. A real-time dynamic monitoring and analysis system for wound healing process according to claim 4, characterized in that: The calculation formula of the guiding culture solution adjustment unit is as follows: in: PY is the adjustment amount of culture medium; If PY is a positive value, it means that the volume of culture medium needs to be increased; If PY is a negative value, it means that the volume of the culture medium needs to be reduced; If PY is 0, it means that there is no need to adjust the volume of the culture medium; KT is the culture medium adjustment coefficient. KT determines the adjustment ratio according to the wound healing rate YH: If the wound healing rate YH is higher than the previous wound healing rate YH old If it increases, it means that the wound heals quickly and the amount of culture medium needs to be reduced to avoid waste. The calculation formula of the culture medium adjustment coefficient K is: If the wound healing rate YH is higher than the previous wound healing rate YH old If it decreases, it means that the wound heals slowly and needs more nutritional support. The calculation formula of the culture medium adjustment coefficient K is: YH old It is the previous wound healing rate, reflecting the result of the wound healing rate YH calculated and output in the previous wound healing monitoring stage.

6. A real-time dynamic monitoring and analysis system for wound healing process according to claim 5, characterized in that: The calculation formula of the guiding temperature adjustment unit is as follows: in: HW is the temperature adjustment amount, and HW reflects the amount by which the temperature in the incubator needs to be adjusted; If HW is a positive value, it means that the temperature in the incubator needs to be increased; If HW is a negative value, it means that the temperature in the incubator needs to be reduced; If HW is 0, it means that there is no need to adjust the temperature in the incubator; PY old The previous culture medium adjustment amount reflects the result of the culture medium adjustment amount PY calculated and output in the previous wound healing monitoring stage; W opt To cultivate the expected temperature; W is the current temperature; W max Adjust the maximum value for the temperature; W min Adjust the minimum value for temperature; [(PY-PY old )×(W opt -W)] calculates the change in culture fluid demand and the adjustment amount of the temperature difference, and the adjustment amount reflects the incubator temperature adjustment demand caused by the change in culture fluid demand.

7. A real-time dynamic monitoring and analysis system for wound healing process according to claim 6, characterized in that: The adjustment instruction of the culture solution transmitted by the central control module to the culture solution adjustment module through the monitoring transmission module is the result of the culture solution adjustment amount PY; The instruction for adjusting the temperature in the incubator transmitted by the central control module to the temperature adjustment module through the monitoring transmission module is a result of the temperature adjustment amount HW.

8. A real-time dynamic monitoring and analysis system for wound healing process according to claim 6, characterized in that: The expected culture temperature W opt The expected cell survival rate XB opt The setting and calculation are similar. According to the three stages of wound healing, namely, primary healing, secondary healing, and tertiary healing, the current temperatures W with an upward trend in the three stages are collected regularly and respectively, and the current temperatures W collected in the three stages are averaged respectively. The calculation formula for the average calculation of the current temperature W is as follows: in: N is the collection volume; W i is the i-th temperature; The current temperature W is collected in three stages and the expected cultivation temperature W is calculated. opt The calculation formula is the same.

9. A real-time dynamic monitoring and analysis system for wound healing process according to claim 2, characterized in that: The equipment used by the central control module includes a control system; The equipment used by the monitoring transmission module includes data transmission equipment; The equipment used in the wound regular collection module includes high-precision measurement equipment, image processing software, and cell viability detection equipment; The equipment used by the monitoring and adjustment module includes computer equipment; The equipment used in the culture solution adjustment module includes culture solution adjustment equipment; The equipment used in the temperature adjustment module includes an incubator temperature adjustment device.

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