Microbial complex microbial inoculant, method for promoting rapid maturity and decomposition of organic fertilizer and application of microbial complex microbial inoculant

Abstract

The invention relates to the technical field of fertilizers, in particular to a microbial complex microbial inoculant, a method for promoting quick maturity and decomposition of an organic fertilizer and application of the microbial complex microbial inoculant. The method comprises the steps that compost containing cow dung and corn straw is inoculated with a microbial complex microbial inoculant formed by brevundimonas AFBI, alcaligenes faecalis AFBII and achromobacter AFBIII, fermentation is carried out under the natural condition, the effective colony count ratio of the brevundimonas AFBI to the alcaligenes faecalis AFBII to the achromobacter AFBIII in the microbial complex microbial inoculant is 1: 1: 1, and the ratio of the effective colony count of the alcaligenes faecalis AFBII to the effective colony count of the achromobacter AFBIII in the microbial complex microbial inoculant is 1: 1: 1. The cow dung and the straw are mixed according to the dry weight ratio of 3: 1; the microbial composite inoculant developed by the invention can significantly improve the degradation efficiency of organic wastes, accelerate the composting process, shorten the composting period and improve the stability and fertility of compost products, and provides a new technical support for resource utilization of agricultural wastes.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers, and specifically relates to a microbial complex bacterium agent, a method for promoting the rapid ripening and decomposition of organic fertilizers, and applications thereof. Background Art

[0002] As a major agricultural production country, in recent years, with the rapid development of the planting and breeding industries in China, China has become the country with the largest output of agricultural waste in the world. According to statistics, the annual output of straw in China in 2022 was nearly 977 million tons, of which corn straw was 340 million tons. China has rich straw resources, but the comprehensive utilization amount is only about 662 million tons, and the comprehensive utilization rate is less than 70%. At the same time, the global livestock industry is developing rapidly, leading to a steady increase in the amount of livestock manure. It is estimated that the annual global output of livestock and poultry manure is about 6.252 billion tons, of which about 18.22% comes from China. However, due to insufficient comprehensive utilization, a large number of agricultural waste organic matter resources that have not been recycled and harmlessly treated are often randomly stacked, discarded, and burned, which not only causes soil pollution, water pollution, and resource waste, but may even lead to serious ecological and environmental problems. With the rapid development of China's agricultural industry, agricultural waste is increasing year by year. Compared with the production speed, the recovery rate of this resource-rich waste is negligible. As a huge treasure house of organic matter and nutrient resources, straw and livestock manure are rich in nutrient elements such as nitrogen, phosphorus, and potassium, and have a rich organic matter content. However, livestock manure also contains many pollutants, including toxic gases such as ammonia and hydrogen sulfide, and pathogenic microorganisms such as bacteria, which will cause serious pollution to the atmosphere and affect the health of breeding livestock and humans. Therefore, studying the current situation and development path of agricultural waste treatment is an important means to solve environmental problems and achieve sustainable development.

[0003] Composting is a common method for converting agricultural waste into organic fertilizers. It uses aerobic microorganisms (such as bacteria, actinomycetes, and fungi) under suitable conditions of ventilation, oxygen supply, temperature, moisture content, pH value, C / N, etc. to effectively promote the conversion of biodegradable organic matter into stable humus. However, traditional composting has problems such as a long fermentation cycle, slow degradation of organic matter, low degree of humification, and safety in using compost products, which have become the key factors restricting the industrialization of composting. In addition, agricultural waste usually contains a relatively high level of refractory organic carbon components such as lignin, hemicellulose, and cellulose. Due to its complex structure and physicochemical composition, it becomes the most stable organic compound in composting, thereby resulting in a low composting fermentation rate and hindering the formation of humus. Summary of the Invention

[0004] Aiming at the deficiencies of the above-mentioned existing technologies, the present invention aims to provide a microbial complex bacterium agent, a method for promoting the rapid ripening and decomposition of organic fertilizers, and applications thereof to improve the composting fermentation rate.

[0005] To solve the above problems, the present invention adopts the following technical solutions:

[0006] A microbial composite bacterium agent, comprising Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ and Achromobacter sp. AFBⅢ; wherein, Brevundimonas sp. AFBⅠ was deposited in the China General Microbiological Culture Collection Center on January 14, 2025, with the deposit number of CGMCC No. 33411; Alcaligenes faecalis AFBⅡ was deposited in the China General Microbiological Culture Collection Center on January 14, 2025, with the deposit number of CGMCC No. 33412; Achromobacter sp. AFBⅢ was deposited in the China General Microbiological Culture Collection Center on January 14, 2025, with the deposit number of CGMCC No. 33413.

[0007] An accelerator for rapid ripening of organic fertilizer, comprising the above-mentioned microbial composite bacterium agent.

[0008] An organic fertilizer, comprising the above-mentioned accelerator.

[0009] A method for promoting rapid ripening of organic fertilizer, comprising inoculating the above-mentioned microbial composite bacterium agent into a compost containing cow dung and corn straw, and fermenting under natural conditions.

[0010] As an implementable mode, it further comprises turning the compost inoculated with the microbial composite bacterium agent every three days during the 30 days before inoculation, and turning the compost every five days after 30 days of inoculation.

[0011] As an implementable mode, the preparation method of the microbial composite bacterium agent comprises inoculating Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ and Achromobacter sp. AFBⅢ into conical flasks containing LB medium respectively, and culturing them on a shaker at room temperature for 48 hours, with the shaker speed of 200 rpm / min; the LB liquid medium is 10 g of peptone, 5 g of yeast extract, 10 g of NaCl, and the volume is made up to 1000 mL with distilled water; sterilizing at 121 °C under high-temperature and high-humidity conditions for 20 min;

[0012] After culturing on the shaker, the colony count is measured to be 1.0×10 8 CFU·mL -1 , preparing a fermentation tank medium with peptone as the substrate, after high-pressure sterilization, mixing the bacterial solutions in a ratio of 1:1:1 and then inoculating them into the fermentation tank for fermentation culture for 48 hours, and measuring that the colony count meets the standard to obtain a liquid microbial composite bacterium agent.

[0013] As an implementable mode, the effective colony number ratio of Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ and Achromobacter sp. AFBⅢ in the microbial composite bacterium agent is 1:1:1.

[0014] As an implementable mode, the method for preparing the compost includes cutting corn straw into small segments of 0.5 - 1 cm, naturally air - drying cow dung and corn straw, mixing cow dung and straw at a dry weight ratio of 3:1 (w / w), and adjusting the initial moisture content of the compost to 60 - 65% with distilled water.

[0015] As an implementable mode, the addition amount of the microbial complex bacterium agent is 5%.

[0016] As an implementable mode, the fermentation time under natural conditions is not less than 30 days.

[0017] Application of a microbial complex bacterium agent in promoting rapid ripening and decomposition of organic fertilizer.

[0018] As an implementable mode, the microbial complex bacterium agent promotes the increase of the compost fermentation temperature and extends the thermophilic stage of the compost fermentation.

[0019] As an implementable mode, the microbial complex bacterium agent reduces the nitrogen loss of the compost, increases the humic acid content and cellulose degradation rate of the compost.

[0020] The beneficial effects of the present invention are as follows: The microbial complex bacterium agent provided by the present invention can significantly improve the degradation efficiency of organic waste, accelerate the composting process, shorten the composting cycle, and enhance the stability and fertility of the compost product. It provides new technical support for the resource utilization of agricultural waste. By optimizing the composting process and the application of the bacterium agent, it can improve the composting efficiency, accelerate the humification process, and promote the efficient production of organic fertilizers, providing effective technical support for realizing the resource utilization of agricultural waste. Description of the Drawings

[0021] Figure 1 It is a curve graph of temperature change in aerobic composting of cow dung and corn straw.

[0022] Figure 2 It is a curve graph of moisture content change in aerobic composting of cow dung and corn straw.

[0023] Figure 3 It is a curve graph of pH change in aerobic composting of cow dung and corn straw.

[0024] Figure 4 It is a curve graph of total carbon content change in aerobic composting of cow dung and corn straw.

[0025] Figure 5 It is a curve graph of total nitrogen content change in aerobic composting of cow dung and corn straw.

[0026] Figure 6 It is a curve graph of carbon - nitrogen ratio (C / N ratio) change in aerobic composting of cow dung and corn straw.

[0027] Figure 7 It is a bar chart showing the change in humic acid content during the aerobic composting of cow dung and corn straw.

[0028] Figure 8 It is a bar chart showing the change in cellulose content during the aerobic composting of cow dung and corn straw. Specific implementation manners

[0029] The present invention will be further described in detail below in conjunction with specific embodiments.

[0030] It should be noted that these embodiments are only used to illustrate the present invention, rather than limiting the present invention. Any simple improvement of the method under the premise of the concept of the present invention falls within the scope of protection required by the present invention.

[0031] Embodiment 1

[0032] The microbial composite bacterium agent of the present invention includes Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ, and Achromobacter sp. AFBⅢ.

[0033] Among them, Brevundimonas sp. AFBⅠ was deposited on January 14, 2025, at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit address being No. 3, Courtyard 1, Beichen Road, Chaoyang District, Beijing, and the deposit number being CGMCC No. 33411.

[0034] Alcaligenes faecalis AFBⅡ was deposited on January 14, 2025, at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit address being No. 3, Courtyard 1, Beichen Road, Chaoyang District, Beijing, and the deposit number being CGMCC No. 33412.

[0035] Achromobacter sp. AFBⅢ was deposited on January 14, 2025, at the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the deposit address being No. 3, Courtyard 1, Beichen Road, Chaoyang District, Beijing, and the deposit number being CGMCC No. 33413.

[0036] Preparation of the composite bacterium agent

[0037] The degrading bacteria are three cellulose-degrading bacteria (Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ, Achromobacter sp. AFBⅢ) screened and preserved from cow dung compost, corn straw pile and farm humus soil. A strain combination H3 (the effective colony number ratio of Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ, Achromobacter sp. AFBⅢ is 1:1:1) with high efficiency in degrading lignocellulose was screened out, and there is no antagonism among them.

[0038] Brevundimonas sp. AFBⅠ, Alcaligenes faecalis AFBⅡ, and Achromobacter sp. AFBⅢ were respectively inoculated into conical flasks containing 500 mL of LB medium and cultured on a shaker at room temperature for 48 hours, and the shaker speed was 200 rpm / min. LB liquid medium: 10 g of peptone, 5 g of yeast extract, 10 g of NaCl, made up to 1000 mL with distilled water; sterilized by high-temperature moist heat at 121 °C for 20 min.

[0039] After shaking culture, the colony count was measured to be 1.0×10 8 CFU·mL -1 . Using peptone as the substrate to prepare the fermenter medium, after autoclaving, the bacterial solutions were mixed in a ratio of 1:1:1 and then inoculated into the fermenter for fermentation culture for 48 hours, and the colony count was measured to check if it met the standard, obtaining a liquid microbial compound bactericide.

[0040] Example 2

[0041] Aerobic composting experiment of cow dung and corn straw

[0042] The composting raw materials used were fresh cow dung and corn straw. The corn straw was cut into small sections of 0.5 - 1 cm, and the fresh cow dung and corn straw were naturally air-dried in the experimental greenhouse to remove the excess surface moisture. The main physical and chemical properties of the composting materials are shown in Table 1. Cow dung and straw were mixed at a ratio of 3:1 (w / w, dry weight) to reach a carbon-nitrogen ratio of about 25, and the initial composting moisture was adjusted to 60 - 65% with distilled water.

[0043] Table 1 Basic physical and chemical properties of composting materials

[0044]

[0045] Two treatments were set (CK - control group, bactericide H3), and each treatment had 3 replicates. The initial weight of the compost pile was 200 kg, and the volume of each pile was approximately 1 m × 0.8 m × 1 m (length × width × height). The concentration of the functional microbial inoculum was 1.0×10 8 CFU·mL -1 , and inoculated at an addition amount of 5%. The CK group was inoculated with an equal amount of deionized water.

[0046] At the beginning of composting, cover the compost pile with a layer of plastic film to ensure its temperature rise process. Then, carry out composting fermentation under natural conditions. In the first 30 days of composting, turn the pile manually every 3 days to ensure sufficient ventilation; after the 30th day, turn the pile every 5 days. Measure the temperatures of the upper, middle, and lower layers of the compost pile at 8:00 and 16:00 every day, and use the average temperature measured every day for further analysis. At the same time, monitor the ambient temperature. Sampling is carried out on the 1st, 3rd, 5th, 10th, 15th, 20th, 25th, 30th, 35th, 40th, 45th, 50th, 55th, and 60th days of composting. The sampling depths are 0.25 m, 0.5 m, and 0.75 m from the top of the compost pile respectively. Three sub-samples are taken from each layer using the five-point sampling method. After all the sub-samples are evenly mixed, they are the composite samples for this period. Store the composite samples in a 4°C refrigerator for the determination of physicochemical indexes.

[0047] Example 3

[0048] Determination and analysis of physicochemical indexes

[0049] As can be seen from Figure 1 , in the initial stage of composting (the first 5 days), the temperatures of all treatment groups rose rapidly. Especially in the H3 complex inoculant group, the temperature rise rate was faster, significantly higher than that of the control group. This indicates that inoculating the inoculant can accelerate the metabolism of the compost materials and quickly enter the thermophilic stage (temperature exceeding 50°C). The addition of the complex inoculant significantly increased the compost temperature (5 - 10°C) and extended the thermophilic stage (5 - 7 days). At the same time, the H3 treatment group remained at a high temperature close to 50°C around the 20th day, which helped to more effectively kill the pathogenic bacteria, eggs, and weed seeds in the compost and promote the degradation of difficult-to-decompose organic matters (such as lignin and cellulose).

[0050] See Figure 2 , as the composting progresses, the moisture content of all treatment groups shows a downward trend. The decrease in moisture is usually related to the rapid decomposition of organic matter by microorganisms. Due to the active metabolism of microorganisms, the moisture in the compost is quickly utilized, promoting the decomposition of organic matter. At the same time, due to the increase in compost temperature, the water evaporation rate also accelerates, resulting in a gradual decrease in the moisture content. In the H3 complex inoculant group, due to the strong metabolism of microorganisms, it promoted the degradation of organic matter and accelerated the utilization efficiency of moisture, resulting in a 20% decrease in the moisture content.

[0051] See Figure 3, the pH value of each treatment group increased rapidly and approached around 8.2 - 8.5 at the initial stage of composting. This might be because ammonia gas was produced during the decomposition of organic matter at the initial stage of composting, and the release of ammonia would increase the pH value of the composting environment. Among them, the H3 group was significantly higher than the CK group. Then, the pH value showed an obvious downward trend and approached 7.6 - 7.8. Among them, the decline amplitude of the H3 group was significantly higher than that of the control group. This was because during the microbial metabolism process, some organic matter decomposed to produce organic acids (such as acetic acid and lactic acid), and these acidic substances caused the pH of the compost to decrease. In the later stage of composting, the pH value increased again and reached 8.0 - 8.3. It might be due to the gradual decomposition of organic acids, and the remaining organic matter was degraded to produce alkaline substances such as ammonia or carbonates. Since the organic acids were consumed, the alkaline substances in the composting environment increased, resulting in the recovery of pH.

[0052] See Figure 4 , at the initial stage, the total carbon content was relatively high, and each group was close to 350 g / kg. With the progress of composting, the total carbon content of all treatment groups gradually decreased. Especially in the first 20 days, the decline was more obvious. The total carbon content of the H3 group decreased to about 270 g / kg, which was significantly lower than that of the control group. After that, the carbon content tended to be stable with less fluctuation. It might be that during the composting process, microorganisms decomposed organic matter, and carbon was consumed as an energy source. The composting temperature was relatively high in the early stage, microorganisms were active, and organic matter was rapidly degraded, releasing carbon dioxide, resulting in a rapid decrease in the total carbon content. With the progress of composting and entering the humus stage, the refractory components in organic matter began to dominate the degradation process, so the decline rate of total carbon gradually slowed down.

[0053] See Figure 5 , different from the total carbon, the total nitrogen content was relatively low at the initial stage. The control group was about 15 g / kg, and the H3 group was slightly higher. During the composting process, the total nitrogen content gradually increased, reached a peak around the 30th - 40th day, then fluctuated slightly, and finally tended to be stable between 20 - 23 g / kg. During the composting process, the transformation of nitrogen was mainly caused by microbial activities. With the decomposition of organic matter, part of the nitrogen might be transformed into ammonium nitrogen and nitrate nitrogen through nitrification and denitrification in the nitrogen cycle. At the same time, there was nitrogen fixation in the composting system, resulting in a continuous increase in the total nitrogen content. Compared with the control group, the addition of the composite microbial agent could significantly reduce the nitrogen loss by about 4.54% and improve the nitrogen fixation ability.

[0054] See Figure 6, In the initial stage, the C / N ratio of each treatment group was relatively high. As the composting process progressed, the C / N ratio decreased significantly, especially in the first 30 days with a relatively fast decline rate, and then gradually leveled off, finally remaining between 12 and 14. The decrease in the carbon-nitrogen ratio during composting mainly reflected the rapid consumption of carbon and the accumulation of nitrogen. In the initial stage with vigorous microbial activity, carbon was preferentially consumed as an energy source, resulting in a rapid decrease in the C / N ratio. As carbon decreased, the relative concentration of nitrogen increased, which was also consistent with the upward trend of the total nitrogen content. When the composting reached the later stage, the carbon-nitrogen ratio tended to be stable, indicating that the degradation of organic matter entered a relatively stable stage and the degradation by microorganisms slowed down. It can be seen from the figure that compared with the control group, inoculating the compound microbial agent could significantly accelerate the degradation of organic matter and reduce nitrogen loss.

[0055] See Figure 7 , In the initial stage of composting, easily decomposable organic matters such as sugars and proteins were rapidly degraded by microorganisms, generating a large amount of fulvic acid. In the H3 treatment group, due to the addition of microbial degradation bacteria, the initial microbial activity was stronger, and the generation rate of fulvic acid was faster, resulting in a relatively lower humic acid content. By the thermophilic stage, the humic acid content began to increase significantly. The H3 group reached 8.68 g / kg, which was 1.34 higher than that of the control group. The increase in humic acid indicated an accelerated humification process. Complex organic matters such as cellulose and lignin were gradually decomposed, generating more humic acid, and the stability of the compost was further enhanced. By the maturity stage, the humic acid content continued to increase. The H3 group reached 10.59 g / kg, significantly higher than 8.15 g / kg of the control group. This trend indicated that the addition of the compound microbial agent accelerated the formation of humus. The humic acid content in the H3 group was 2.44 g / kg higher than that of the control group, an increase of approximately 29.94%, and the maturity and stability of the compost product were significantly improved.

[0056] See Figure 8, during the initial and mesophilic phases of composting, the cellulose content in the control group and the H3 complex inoculant group changed little, with a small difference, indicating that in the initial stage, the rate of cellulose degradation was slow, microbial activity had just started to become active, and complex organic matter had not been significantly decomposed. During the thermophilic phase, the rate of cellulose degradation in the control group began to slow down, and the cellulose content decreased slowly, while the cellulose degradation in the H3 group accelerated significantly. The addition of the H3 complex inoculant accelerated microbial metabolic activity and promoted the rapid degradation of cellulose. During the maturity phase, the cellulose content in both the control group and the H3 group decreased further, but the degradation effect in the H3 group was more significant, with the cellulose content dropping to 35.83 mg / g, far lower than 53.78 mg / g in the control group. Generally speaking, the cellulose content in the H3 treatment group decreased from the initial 104.97 g / kg to 35.83 g / kg, with a degradation rate of approximately 66.2%, showing a significant degradation effect. In contrast, the cellulose content in the control group decreased from 106.86 g / kg to 53.78 g / kg, with a degradation rate of approximately 49.7%, and the degradation rate was slower. The H3 complex inoculant increased the cellulose degradation efficiency by approximately 16.5%, accelerated the decomposition of organic matter, and promoted the maturity of the compost.

[0057] Compared with the control group, the H3 group had a faster temperature rise, the thermophilic stage was extended by 5 - 7 days, nitrogen loss was reduced by 4.54%, the humic acid content was increased by 29.94%, and the cellulose degradation rate was increased by 16.5%. In terms of the physical and chemical changes of the compost, the changes in the total carbon content and total nitrogen content in the H3 group indicated that microbial activity promoted the degradation of organic matter and nitrogen transformation. At the same time, indicators such as pH value and C / N ratio also showed a trend conducive to the improvement of soil fertility.

[0058] In summary, the complex inoculant developed in this invention can significantly improve the degradation efficiency of organic waste, accelerate the composting process, shorten the composting cycle, and enhance the stability and fertility of the compost product. It provides new technical support for the resource utilization of agricultural waste. By optimizing the composting process and the application of the inoculant, it can improve the composting efficiency, accelerate the humification process, and promote the efficient production of organic fertilizers, providing effective technical support for the realization of the resource utilization of agricultural waste.

[0059] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described by referring to the preferred embodiments of the present invention, those of ordinary skill in the art should understand that various changes can be made in form and details without departing from the spirit and scope of the present invention defined by the appended claims.

Claims

1. A microbial composite agent, characterized in that: Including Brevundimonas AFBⅠ, Alcaligenes faecalis AFBⅡ and Achromobacter AFBⅢ; among them, Brevundimonas AFBⅠ was deposited in the General Microbiology Center of China Culture Collection of Microorganisms on January 14, 2025, with the deposit number CGMCC No.33411; Alcaligenes faecalis AFBⅡ was deposited in the General Microbiology Center of China Culture Collection of Microorganisms on January 14, 2025, with the deposit number CGMCC No.33412; Achromobacter AFBⅢ was deposited in the General Microbiology Center of China Culture Collection of Microorganisms on January 14, 2025, with the deposit number CGMCC No.33413.

2. An organic fertilizer quick-maturing and ripening promoter, characterized in that: It includes the microbial composite agent as described in claim 1.

3. An organic fertilizer, characterized in that: The accelerator according to claim 2 is included.

4. A method for promoting rapid maturation and decomposition of organic fertilizer, characterized in that: The microbial composite agent according to claim 1 is inoculated into compost containing cow dung and corn stalks, and fermented under natural conditions.

5. The method for promoting rapid maturation and decomposition of organic fertilizer according to claim 4, characterized in that: The method also includes turning the compost inoculated with the microbial composite agent every three days in the 30 days before inoculation, and turning the compost every five days after 30 days of inoculation.

6. The method for promoting rapid maturation and decomposition of organic fertilizer according to claim 4, characterized in that: The preparation method of the microbial composite agent comprises the following steps: inoculating Brevundimonas AFBⅠ, Alcaligenes faecalis AFBⅡ, and Achromobacter AFBⅢ into a conical flask containing LB culture medium, and culturing them at room temperature for 48 hours on a shaking table, with a shaking speed of 200 rpm / min; the LB liquid culture medium is 10 g of peptone, 5 g of yeast extract, and 10 g of NaCl, and the volume is fixed to 1000 mL with distilled water; and sterilizing them at high temperature and wet heat at 121°C for 20 min; After shaking culture, the colony count was determined to be 1.0×10 8 CFU·mL -1 , prepare the fermentation tank culture medium with peptone as the substrate. After high-pressure sterilization, mix the bacterial liquid at a ratio of 1:1:1 and then inoculate it into the fermentation tank for fermentation culture for 48 hours. The colony count is determined to meet the standard to obtain a liquid microbial composite agent.

7. The method for promoting rapid maturation and decomposition of organic fertilizer according to claim 4, characterized in that: The compost preparation method comprises cutting corn stalks into small segments of 0.5-1 cm, naturally air-drying cow dung and corn stalks, mixing cow dung and stalks at a dry weight ratio of 3:1, and adjusting the initial compost moisture to 60-65% with distilled water.

8. Use of the microbial composite agent described in claim 1 in promoting rapid maturation and decomposition of organic fertilizer.

9. The use according to claim 8, characterized in that: The microbial composite agent promotes the increase of compost fermentation temperature and prolongs the thermophilic stage of compost fermentation.

10. The use according to claim 8, characterized in that: The microbial composite agent reduces nitrogen loss in compost and increases the humic acid content and cellulose degradation rate of the compost.

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