Device and method for detecting vigor of crop seeds

Abstract

The invention relates to the technical field of seed cultivation detection, in particular to a crop seed vigor detection device and a method thereof.The crop seed vigor detection device comprises a main body, the main body comprises a funnel, a valve, a pressure release valve, a sealing plug and a container, the sealing plug covers the open end of the container, and a closed space is formed to provide an environment for a test; a funnel and a pressure release valve are mounted on the sealing plug, the funnel is used for containing a liquid reactant, and a valve is arranged on the funnel and used for controlling the reaction process; the seed containing part is used for containing seeds and deforms to extrude the seeds under the change of air pressure; the reactant containing part is located in the container and used for containing solid reactants; according to the design, composite stress simulation of seed vigor is realized on the basis of a simple structure, including damage to seed coats by high pressure, high temperature, high humidity and physical means, so that more comprehensive and efficient seed vigor detection within a short time is realized.

Description

Technical Field

[0001] The present invention relates to the technical field of seed cultivation and detection, and particularly relates to a device and method for detecting the vigor of crop seeds. Background Art

[0002] The design and cultivation of new super-high-yield, stress-tolerant and widely adaptable maize varieties are carried out through advanced genetic breeding techniques and molecular biological means, combined with environmental adaptability evaluation, to cultivate new maize varieties with super-high yield, strong stress tolerance and wide adaptability. The project will deeply study the genetic basis of maize, excavate key genes controlling important traits such as yield and stress resistance, and use these genes for molecular marker-assisted selection and gene editing to achieve the rapid and accurate cultivation of new maize varieties. At the same time, the project will also carry out extensive field trials to evaluate the performance of new varieties in different ecological environments to ensure their stability and reliability in actual production. The implementation of this project will provide strong technical support for the development of China's maize industry.

[0003] After retrieval, CN219248534U discloses a device for detecting the stress of crop seed vigor, which relates to the field of seed detection equipment, including a detection chamber, a temperature regulation system, a controller, a culture rack and a temperature feedback system. The temperature regulation system is fixedly arranged in the detection chamber and is used to regulate the temperature in the detection chamber. The controller is communicatively connected with the temperature regulation system, and the controller can control the temperature regulation system to regulate the temperature in the detection chamber. The culture rack is fixedly arranged in the detection chamber, and the culture rack is used to place seeds. The temperature feedback system is fixedly arranged in the detection chamber, and the temperature feedback system is communicatively connected with the controller. The temperature feedback system is used to detect the temperature information in the detection chamber and transmit the temperature information to the controller. The controller can control the temperature regulation system to regulate the temperature in the detection chamber according to the temperature information, support the simulation of single or compound stresses such as low temperature and high temperature, and is applicable to the detection of the storage tolerance and stress resistance of seeds such as maize and soybean.

[0004] Seed vigor detection is an important means to evaluate the germination ability, stress resistance and production potential of seeds. The commonly used methods can be divided into the following categories: Physical detection methods, including germination tests, X-ray detection and seed weight screening, evaluate seed vigor through germination rate, internal structure imaging and seed morphology respectively.

[0005] Biochemical detection methods, such as tetrazolium staining and conductivity measurement, use cell activity and membrane integrity to quickly judge seed vigor and are applicable to most crops.

[0006] Stress simulation methods, through accelerated aging, cold soaking or salt stress tests, evaluate the stress resistance of seeds under high temperature, low temperature or drought conditions.

[0007] Physiological index detection, such as measuring respiratory intensity and free radical scavenging ability, reflects the seed metabolic level and anti-aging ability, and helps with vitality analysis.

[0008] Modern technologies, such as near-infrared spectroscopy and AI image analysis, provide efficient and non-destructive means for vitality assessment. Combining multiple methods can comprehensively guide agricultural production.

[0009] Among them, the adversity simulation method is the most direct and effective technical means to evaluate the survival and germination ability of seeds under extreme environments. By simulating adverse environmental conditions, such as high temperature, low temperature, drought, or salt stress, it can accurately predict the challenges that seeds may encounter during actual planting and screen out seeds with excellent stress resistance.

[0010] Common means include: 1. Accelerated aging test Simulate long-term storage through a high-temperature and high-humidity environment to evaluate the storage tolerance and anti-aging ability of seeds. The decline in germination rate reflects the seed vitality.

[0011] 2. Cold soaking treatment test Simulate low-temperature and humid conditions to detect the cold tolerance of seeds. The germination rate and rotting ratio are used to judge their cold resistance performance.

[0012] 3. Salt / drought stress test Use NaCl and PEG solutions respectively to simulate saline-alkali and drought environments, and evaluate the salt tolerance and drought resistance potential of seeds. The key indicators are the germination rate and radicle elongation.

[0013] 4. High-temperature stress test Test the heat tolerance of seeds through a high-temperature germination chamber. The morphological changes of seedlings reveal their adaptability to extreme temperatures.

[0014] 5. Submergence stress test Simulate a hypoxic environment to detect the waterlogging tolerance of seeds. The normal germination rate and the proportion of deformed seedlings reflect the anti-hypoxic ability.

[0015] However, with the continuous progress of biotechnology, the overall cycle of conducting experiments on seeds through traditional methods is relatively long. Using a composite adversity simulation device as shown in CN219248534U, although its structural modules are complex, the operation is cumbersome and the cost is high. Therefore, we propose a detection device and method for the vitality of crop seeds. Summary of the Invention

[0016] The purpose of the present invention is to provide a detection device and method for the vitality of crop seeds.

[0017] To achieve the above purpose, the present invention adopts the following technical solutions: A detection device and method for the vitality of crop seeds, including: The main body, which includes a funnel, a valve, a pressure relief valve, a sealing plug, and a container. The sealing plug covers the open end of the container to form a sealed space for providing an environment for the test. The funnel and the pressure relief valve are installed on the sealing plug. The funnel is used to hold liquid reactants, and the funnel is provided with a valve for controlling the reaction process. The seed holding part is used to hold seeds and deforms under the change of air pressure to squeeze the seeds. The reactant holding part is located inside the container and is used to hold solid reactants. The holding part includes a tray. The liquid reactant contacts the solid reactant to react, generating carbon dioxide, water, and heat, causing the air pressure and temperature to rise.

[0018] Preferably, the seed holding part includes a C-shaped airbag, a shrinking top wall, a supporting bottom wall, and grooves. The C-shaped airbag is integrally formed by the shrinking top wall and the supporting bottom wall, with a hollow structure in the middle. The supporting bottom wall has a structure that is thick in the middle and thin at both ends, facilitating the C-shaped bending and shrinking at both ends after the pressure increases. The thickness of the shrinking top wall is less than that of the supporting bottom wall. The supporting bottom wall is distributed with several grooves to facilitate increasing the friction force to squeeze the seed coat.

[0019] Preferably, the bottom of the C-shaped airbag has an installation structure. The seed holding part is installed in the container through the installation structure. The installation structure includes a bracket and a slot. The bracket is adhered to the supporting bottom wall, and the bracket is inserted into the slot, and the slot is provided on the inner wall of the container.

[0020] Preferably, the outside of the container is provided with heat insulation cotton.

[0021] Preferably, the tray is located at the bottom inside the container and is below the C-shaped airbag.

[0022] Preferably, the tray is placed on the convex ring in the middle inside the container and is above the C-shaped airbag. The bottom of the tray has through holes to facilitate the generated water droplets to fall onto the seeds to create a high-humidity environment.

[0023] Preferably, convex ribs are integrally provided on the shrinking top wall of the C-shaped airbag. The convex ribs separate the seeds, and protrusions are integrally provided on the convex ribs to increase the friction force.

[0024] Preferably, the liquid reactant is dilute hydrochloric acid.

[0025] Preferably, the solid reactant is marble.

[0026] The present invention has at least the following beneficial effects: On the basis of a simple structure, this design realizes the composite stress simulation of seed vigor, including high pressure, high temperature, high humidity, and the use of physical means to damage the seed coat, so as to achieve a more comprehensive and efficient detection of seed vigor in a short time. Among them, the high-pressure environment simulates the impact of soil compaction on seed germination, the high-temperature environment helps to reveal the tolerance of seeds under high-temperature conditions, and the high-humidity environment simulates the humidity conditions under waterlogging conditions, further enhancing the authenticity of the simulation. At the same time, by moderately damaging the seed coat through physical means, it can not only promote seed water absorption but also simulate to a certain extent the mechanical damage that seeds may suffer in the natural environment, thus facilitating a more comprehensive evaluation of seed vigor and stress resistance in the later stage.

[0027] The carbon dioxide released during the process can inhibit the further improvement of seed respiration. During the detection of seed vigor, through a specific device design, the carbon dioxide released during the process can be cleverly used to inhibit the respiration of seeds. This mechanism not only helps to slow down the metabolic rate of seeds but also simulates certain stress conditions in the natural environment to a certain extent, such as a low-oxygen environment. By inhibiting seed respiration, it is convenient to observe the survival ability and adaptability of seeds in adversity in the later stage, thereby further evaluating their vigor level. This design not only improves the accuracy and comprehensiveness of seed vigor detection but also provides strong support for subsequent seed screening and breeding work. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0029] Figure 1 It is a schematic diagram of the present invention; Figure 2 It is a sectional view of the present invention; Figure 3 It is an enlarged schematic view of part A of the present invention; Figure 4 It is a schematic diagram of the second embodiment of the present invention; Figure 5 It is a schematic diagram of the third embodiment of the present invention.

[0030] In the figure: 101, funnel; 102, valve; 103, pressure relief valve; 104, sealing plug; 105, container; 201, C-shaped airbag; 202, shrinking top wall; 203, supporting bottom wall; 204, groove; 205, convex rib; 206, protrusion; 301, bracket; 302, card slot; 401, tray; 402, through hole; 403, convex ring. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] In order to make the objectives, technical solutions and advantages of the present invention more clear and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention. Embodiment

[0032] Refer to Figures 1-3 , a detection device and method for the vigor of crop seeds, comprising: The main body is composed of a funnel 101, a valve 102, a pressure relief valve 103, a sealing plug 104 and a container 105. The sealing plug 104 is placed at the open end of the container 105 to form a closed space, providing a necessary environment for the experiment. The outside of the container 105 is covered with heat-insulating cotton to maintain a constant temperature condition. A funnel 101 and a pressure relief valve 103 are installed on the sealing plug 104. The funnel 101 is used to load liquid reactants, and the valve 102 on the funnel 101 is used to precisely control the reaction process.

[0033] A seed holding part, which is designed to hold seeds and squeeze the seeds through deformation when the air pressure changes. The seed holding part includes a C-shaped airbag 201, a shrinking top wall 202, a supporting bottom wall 203 and a groove 204. The C-shaped airbag 201 is integrally formed by the shrinking top wall 202 and the supporting bottom wall 203, and the middle part is a hollow structure. The supporting bottom wall 203 is designed to be thick in the middle and thin at both ends, so that both ends can be bent and shrunk in a C shape when the pressure increases. The thickness of the shrinking top wall 202 is less than that of the supporting bottom wall 203, and a number of grooves 204 are evenly distributed on the supporting bottom wall 203. These grooves help to increase the friction when contacting the seed coat, so as to squeeze the seeds more effectively.

[0034] A holding part for reactants, which is arranged inside the container 105 and is used to hold solid reactants. The holding part includes a tray 401, in which the liquid reactants contact the solid reactants and react to produce carbon dioxide, water and heat, resulting in an increase in air pressure and temperature.

[0035] The bottom of the C-shaped airbag 201 is equipped with an installation structure, and the seed holding part is fixed inside the container 105 through this installation structure. The installation structure is composed of a bracket 301 and a card slot 302. The bracket 301 is pasted on the supporting bottom wall 203, and the bracket 301 is inserted into the card slot 302, and the card slot 302 is arranged on the inner wall of the container 105.

[0036] The tray 401 is located at the bottom inside the container 105 and is below the C-shaped airbag 201.

[0037] The material of the C-shaped airbag 201 is rubber.

[0038] Specifically, the liquid reactant is dilute hydrochloric acid and the solid reactant is marble.

[0039] This solution has the following working process: By opening valve 102 to release dilute hydrochloric acid, the dilute hydrochloric acid comes into contact with marble particles for reaction. The hydrogen ions in the acid combine with carbonate ions to form unstable carbonic acid H2CO3, which then decomposes into carbon dioxide and water. At the same time, heat is released, causing the pressure and temperature inside the container to increase and the environment to become humid. Then, due to the increase in external air pressure, the C-shaped airbag 201 contracts. Due to its structure, it bends into a C shape to wrap and squeeze the seeds inside, damaging the seed coats.

[0040] According to the above working process, it can be seen that the design realizes rapid and comprehensive seed vigor detection by simulating high pressure, high temperature, high humidity, and physical damage to the seed coats. High pressure simulates soil compaction, high temperature tests heat resistance, high humidity simulates flooding humidity, and physical damage simulates natural damage. These methods help to evaluate seed vigor and stress resistance.

[0041] The carbon dioxide released during the detection inhibits seed respiration, slows down metabolism, and simulates a low-oxygen environment. This helps to observe the seed's ability to survive adversity and evaluate the vigor level. The design improves the accuracy and comprehensiveness of the detection, and supports seed screening and breeding. Embodiment

[0042] Refer to Figure 4 , the tray 401 is placed on the convex ring 403 in the middle inside the container 105 and is located above the C-shaped airbag 201. The bottom of the tray 401 has through holes 402 to facilitate the generated water droplets to fall onto the seeds to create a high-humidity environment.

[0043] The design of the tray 401 not only ensures its stable position inside the container but also realizes effective drainage of water droplets through the through holes 402 at its bottom. When the water generated by the reaction of dilute hydrochloric acid and marble particles drips onto the seeds through the through holes, it not only increases the humidity of the environment but also promotes the formation of the microclimate around the seeds, further simulating the seed growth environment under flooding conditions. This consideration in design details makes the flooding stress test closer to reality, thus more accurately evaluating the vigor and stress resistance of seeds under flooding conditions. In addition, the cooperation between the tray 401 and the convex ring 403 also facilitates cleaning and maintenance after the test, improving the practicality and convenience of the entire detection device. Embodiment

[0044] Refer to Figure 5 , on the contraction top wall 202 of the C-shaped airbag 201, convex ridges 205 are integrally provided. The convex ridges 205 separate the seeds, and on the convex ridges 205, protrusions 206 are integrally provided to increase friction.

[0045] This design not only ensures the uniform distribution of seeds in the C-shaped airbag 201 and avoids mutual squeezing between seeds, but also increases the friction between the seeds and the protrusions 206 on the ridge 205, thereby effectively preventing the seeds from moving or rolling during the test. This sophisticated design allows each seed to independently withstand the effects of flooding stress, improving the accuracy and reliability of the test. At the same time, the setting of the ridges and protrusions also enhances the structural strength of the C-shaped airbag, making it more stable when under pressure, further ensuring the smooth progress of the test. In addition, it can provide additional friction when contracting to fully destroy the seed coat.

[0046] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions only describe the principles of the present invention. The present invention may be subject to various changes and improvements without departing from the spirit and scope of the present invention. These changes and improvements fall within the scope of the present invention. The scope of protection claimed by the present invention is defined by the attached claims and their equivalents.

Claims

1. A device for detecting the vitality of crop seeds, characterized in that: include: The main body comprises a funnel (101), a valve (102), a pressure relief valve (103), a sealing plug (104) and a container (105), wherein the sealing plug (104) covers the open end of the container (105) to form a closed space to provide an environment for the test. A funnel (101) and a pressure relief valve (103) are installed on the sealing plug (104); the funnel (101) is used to contain liquid reactants, and the funnel (101) is provided with a valve (102) for controlling the reaction process; The seed holding part is used to hold seeds and deforms and squeezes the seeds due to changes in air pressure; A reactant holding portion, the holding portion is located inside the container (105) and is used to hold solid reactants. The holding portion includes a tray (401). The liquid reactants and the solid reactants come into contact and react to produce carbon dioxide and water as well as heat, thereby increasing the gas pressure and temperature.

2. A device for detecting the vitality of crop seeds according to claim 1, characterized in that: The seed holding portion comprises a C-shaped air bag (201), a shrinkable top wall (202), a supporting bottom wall (203), and a groove (204). The C-shaped air bag (201) is formed integrally by the shrinkable top wall (202) and the supporting bottom wall (203), and the middle part is a hollow structure. The supporting bottom wall (203) is a structure with a thick middle part and thin ends, so that the two ends can be bent and shrunk in a C shape after pressure is increased. The thickness of the shrinkable top wall (202) is smaller than that of the supporting bottom wall (203). The supporting bottom wall (203) is scattered with a plurality of grooves (204) so ​​as to increase friction and squeeze the outer coat of the seed.

3. A device for detecting the vitality of crop seeds according to claim 2, characterized in that: The bottom of the C-shaped airbag (201) has a mounting structure, and the seed holding part is mounted in the container (105) through the mounting structure. The mounting structure comprises a bracket (301) and a slot (302). The bracket (301) is bonded to the supporting bottom wall (203), and the bracket (301) is inserted in the slot (302). The slot (302) is arranged on the inner wall of the container (105).

4. A device for detecting the vitality of crop seeds according to claim 1, characterized in that: The outer side of the container (105) is provided with heat-insulating cotton.

5. The device for detecting the vitality of crop seeds according to claim 1, characterized in that: The tray (401) is located at the bottom of the inner side of the container (105) and below the C-shaped airbag (201).

6. A device for detecting the vitality of crop seeds according to claim 1, characterized in that: The tray (401) is placed on the convex ring (403) in the middle of the inner side of the container (105) and is located above the C-shaped airbag (201). The bottom of the tray (401) has a through hole (402) to facilitate the generated water to drip onto the seeds to create a high humidity environment.

7. The device for detecting the vitality of crop seeds according to claim 1, characterized in that: A convex ridge (205) is integrally provided on the contracted top wall (202) of the C-shaped airbag (201), the convex ridge (205) separates the seeds, and a protrusion (206) is integrally provided on the convex ridge (205) for increasing friction.

8. The device for detecting the vitality of crop seeds according to claim 1, characterized in that: The liquid reactant is dilute hydrochloric acid.

9. The device for detecting the vitality of crop seeds according to claim 1, characterized in that: The solid reactant is marble.

10. A method for detecting the vitality of crop seeds according to any one of claims 1 to 9, characterized in that: include: Opening the valve (102) releases dilute hydrochloric acid, which reacts with the marble to generate carbon dioxide and water, while generating heat, causing the pressure and temperature inside the container to increase, and the humidity to increase. The C-shaped air bag (201) contracts due to the increase in external air pressure, and its structure causes it to bend, wrap around and squeeze the seeds, destroying their outer coats.

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