A method for improving cassava's resistance to bacterial wilt by treating it with sucrose

Abstract

The present invention discloses a method for improving cassava's resistance to bacterial wilt by treating it with sucrose. This method, which belongs to the field of vegetable cultivation, comprises raising 45-day-old cassava seedlings, soaking the cassava seedling bags in a 1-3% (w / v) sucrose aqueous solution for 3-5 days, wherein the sucrose solution covers the cassava seedling bags by 1-2.5 cm. The sucrose used in this invention is a common food additive that is non-polluting and edible. Treating cassava seedlings with it does not cause pollution or chemical residues, has no negative impact on the normal growth of the seedlings, and improves cassava's disease resistance. By optimizing the concentration of the sucrose aqueous solution, a 3% concentration of sucrose solution exhibits the best disease resistance. This method is simple, easy to implement, fast-acting, and highly efficient, and is of great significance to the healthy development of the cassava industry.

Description

Technical Field

[0001] The invention belongs to the technical field of vegetable cultivation, and particularly relates to a method for improving the bacterial wilt resistance of cassava through sucrose treatment. Background Art

[0002] Cassava can be used as both a food crop and an energy crop, so there is a huge demand for cassava in the domestic and international markets. However, the production of cassava is frequently restricted by diseases. Among them, cassava bacterial wilt is a bacterial disease that seriously endangers the world's cassava production. Its pathogenic bacteria is Xanthomonas fasciatus ( Xanthomonas saxonopodis pv. Manihotis, Xam ), Xam It can colonize the xylem vessels of cassava stems and spread long distances along the stems. High temperatures and high humidity are optimal conditions for bacterial wilt outbreaks, especially during typhoon and rainy seasons. The disease can spread rapidly through fields via air, rain, and insects, causing severe damage. Furthermore, the incidence and severity of bacterial wilt are variable and difficult to predict across time and space, making disease prevention and control a significant challenge.

[0003] Sugar is a key substance that reflects a plant's energy levels. Consistently sensing sugar levels and controlling energy supply are crucial for plant survival. Studies have shown that sucrose can regulate the expression of defense-related genes in plants, thereby influencing their disease resistance. However, whether sucrose can modulate disease resistance in cassava has not been reported. Summary of the Invention

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and in the abstract and title of the present invention to avoid obscuring the purpose of this section, the abstract and the title of the invention, and such simplifications or omissions should not be used to limit the scope of the present invention.

[0005] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0006] Therefore, the object of the present invention is to overcome the deficiencies in the prior art and provide a method for improving the resistance of cassava to bacterial wilt by treating with sucrose.

[0007] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0008] Cassava grown to 45 days old is obtained by seedling cultivation, and a cassava seedling bag is immersed in a sucrose aqueous solution with a concentration of 1-3% (w / v), wherein the sucrose aqueous solution covers the cassava seedling bag by 1-2.5 cm.

[0009] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the soaking time is 3 to 5 days.

[0010] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the concentration of the sucrose aqueous solution is 3% (w / v).

[0011] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the seedling cultivation comprises:

[0012] Fill the seedling bag with a matrix obtained by mixing nutrient soil and vermiculite, cut the lignified cassava stems into cassava segments, insert them into the matrix of the seedling bag with the buds facing upward, water the matrix thoroughly, place it in a greenhouse at 25-30°C for growth, expose it to light for 12-14 hours, avoid light for 10-12 hours, and water and fertilize it to allow the cassava to grow to 45 days old.

[0013] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the lignified cassava stems are cut into cassava segments, wherein the cassava segments are 8 to 12 cm in length.

[0014] As a preferred embodiment of the method for improving cassava resistance to bacterial wilt by sucrose treatment according to the present invention, the watering is performed at a rate of 100 to 200 mL of water every three days.

[0015] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the fertilization comprises watering with 50-100 mL of Hua Wu Que aqueous solution every 10 days.

[0016] As a preferred embodiment of the method for improving cassava's resistance to bacterial wilt by sucrose treatment according to the present invention, the concentration of the aqueous solution of huawuque is 1-2 g / L.

[0017] Beneficial effects of the present invention:

[0018] (1) The sucrose in the present invention is a commonly used food additive, is pollution-free and edible, and using it to treat cassava seedlings will not cause pollution and chemical residues, has no negative impact on the normal growth of cassava seedlings, and can improve the disease resistance of cassava.

[0019] (2) The method provided by the present invention for improving the disease resistance of cassava by treating with sucrose is simple, easy to implement, quick in effect and highly efficient. It can quickly improve the resistance of cassava to bacterial wilt and is of great significance to the healthy development of the cassava industry. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0021] Figure 1 The number of bacteria resistant to bacterial wilt in cassava after being treated with sucrose aqueous solutions of different concentrations for 0 day, 2 days, and 5 days in Example 1 of the present invention and Comparative Examples 1 and 2 is shown. DETAILED DESCRIPTION

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0023] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0024] Secondly, the term "one embodiment" or "embodiment" herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The phrase "in one embodiment" appearing in various places throughout this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive of other embodiments.

[0025] The main components of the Hua Wuque aqueous solution used in the present invention are: calculated by mass fraction, 5.97% nitrate nitrogen, 3.92% ammonium nitrogen, 10.11% urea nitrogen, 20% water-soluble phosphorus, 20% water-soluble potassium, 0.07% magnesium, 0.05% EDTA-copper, 0.1% EDTA-iron, 0.1% EDTA-manganese, 0.1% EDTA-zinc, 0.15% boron, 0.002% molybdenum, and 0.0005% EDTA-cobalt.

[0026] The LB solid culture medium used in the present invention comprises the following components: 5 g / L yeast extract, 10 g / L tryptone, 10 g / L sodium chloride, and 20 g / L agar powder, which is sterilized at 121° C. for 20 min.

[0027] The preparation method of the bacterial suspension used in the present invention is as follows: take the carpet grass Xanthomonas bacteria strains preserved in the laboratory, inoculate them on LB solid medium, culture them at 28 ° C for 18 hours, use 1mM MgCl2 solution to wash the bacteria in the medium, and dilute them to OD 600 =0.6 to obtain bacterial suspension.

[0028] Example 1. This example provides a method for improving cassava's resistance to bacterial wilt by treating it with sucrose, specifically:

[0029] Seedling cultivation: Fill a 10cm-diameter seedling bag with a matrix made by mixing nutrient soil and vermiculite in a 1:1 mass ratio. Cut the lignified cassava stems into 10cm-long segments and insert them into the matrix of the seedling bag with the buds facing upwards. After watering the matrix thoroughly, place it at 30°C for 12 hours of light and 12 hours of dark protection. Apply 100mL of water every 3 days and 50mL of a 1g / L solution of Hua Wuque every 10 days.

[0030] Sucrose treatment: Cassava seedlings grown to 45 days old were obtained, and the cassava seedling bags were soaked in sucrose aqueous solutions with concentrations of 1%, 2%, and 3% (w / v) for 5 days, respectively, wherein the sucrose aqueous solution covered the seedling bags by about 1 cm, to obtain bacterial wilt-resistant cassava of this example.

[0031] Comparative Example 1: This comparative example differs from Example 1 in that the concentration of the sucrose aqueous solution is adjusted to 0% (w / v), and the rest of the treatment methods are the same as those in Example 1.

[0032] There were two inoculation points on each cassava lobe treated in Example 1 and Comparative Example 1. 0.1 mL of bacterial suspension was injected into each inoculation point. After inoculation, the leaves were incubated in the dark at 30° C. for 12 h and then incubated in the light. The area of ​​lesions on the cassava leaves was counted 8 days after inoculation. The results are shown in Table 1.

[0033] Table 1 Effects of sucrose aqueous solution concentration on cassava resistance to bacterial wilt

[0034] ,

[0035] It can be clearly seen from Table 1 that 8 days after the cassava treated with deionized water alone was inoculated with bacteria, the area of ​​lesions on the leaves was significantly larger than that of the cassava leaves treated with sucrose aqueous solution, and the area of ​​lesions on the leaves treated with 3% sucrose aqueous solution was reduced by 75% compared with that with 0% concentration. This shows that the treatment with 3% sucrose aqueous solution can significantly improve the resistance of cassava to bacterial wilt.

[0036] Comparative Example 2: This comparative example differs from Example 1 in that the concentrations of the sucrose aqueous solution are adjusted to 5%, 8%, and 10% (w / v), respectively. The remaining processing methods are the same as those in Example 1.

[0037] There are two inoculation points on each cassava crack after treatment in Example 1 and Comparative Examples 1 to 2. 0.1 mL of bacterial suspension is injected into each inoculation point. After the inoculation, the cassava is placed in the dark at 30° C. for 12 hours and then cultured under light. The bacterial counts on the cassava at 0 days, 2 days, and 5 days after inoculation are shown in FIG. Figure 1 shown.

[0038] contrast Figure 1 After treatment with different concentrations of sucrose aqueous solution, the cassava Xam Bacterial count. It can be seen that the bacterial count per unit area of ​​cassava leaves treated with 5% and 8% (w / v) sucrose aqueous solution had no significant difference from that treated with 0% (w / v) sucrose aqueous solution 2 days after inoculation, but was lower than that treated with 0% (w / v) sucrose aqueous solution 5 days after inoculation, but higher than that treated with 1%, 2%, and 3% (w / v) sucrose aqueous solution. The bacterial count per unit area of ​​cassava leaves treated with 10% (w / v) sucrose solution was significantly higher than that of the control group. This shows that treatment with too high a sucrose solution cannot improve the resistance of cassava to Xam 1~3% (w / v) sucrose aqueous solution is the best way to improve the resistance of cassava to Xam Optimal treatment range for resistance.

[0039] In summary, the present invention provides a method for improving cassava disease resistance by treating it with sucrose. Sucrose, a commonly used food additive that is non-polluting and edible, can be used to treat cassava seedlings without causing pollution or chemical residue, without negatively affecting the normal growth of the seedlings, and while improving cassava disease resistance. Furthermore, by optimizing the concentration of the sucrose aqueous solution, a 1-3% concentration of sucrose aqueous solution was found to rapidly improve cassava resistance to bacterial wilt.

[0040] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A method for improving cassava's resistance to bacterial wilt by treating it with sucrose, characterized in that: include, Cassava seedlings grown to 45 days old were obtained, and cassava seedling bags were immersed in a sucrose aqueous solution with a mass volume concentration of 3%, wherein the sucrose aqueous solution covered the cassava seedling bags by 1-2.5 cm; The soaking time is 5 days; The cassava bacterial wilt-resistant pathogen is Xanthomonas axonopodis pv. manihotis .

2. The method for improving cassava resistance to bacterial wilt by sucrose treatment according to claim 1, wherein: The seedling cultivation comprises: Fill the seedling bag with a matrix obtained by mixing nutrient soil and vermiculite, cut the lignified cassava stems into cassava segments, insert them into the matrix of the seedling bag with the buds facing upwards, water the matrix thoroughly, place it at 25-30°C for growth, expose it to light for 12-14 hours, avoid light for 10-12 hours, and water and fertilize it to allow the cassava to grow to 45 days old.

3. The method for improving cassava resistance to bacterial wilt by sucrose treatment as claimed in claim 2, characterized in that: The lignified cassava stems are cut into cassava segments, wherein the cassava segments are 8-12 cm in length.

4. The method for improving cassava resistance to bacterial wilt by treating with sucrose as claimed in claim 2, wherein: The watering was performed with 100-200 mL of water every 3 days.

5. The method for improving cassava resistance to bacterial wilt by treating with sucrose as claimed in claim 2, characterized in that: The fertilization is performed by pouring 50-100 mL of the Huawuque water solution every 10 days.

6. The method for improving cassava resistance to bacterial wilt by treating with sucrose as claimed in claim 5, characterized in that: The concentration of the Hua Wuque aqueous solution is 1-2 g / L.

Images