A miticide and its use

Abstract

The application belongs to the technical field of pesticide acaricidal technology, and discloses an acaricide and application thereof. The acaricide comprises active ingredient A and active ingredient B. The active ingredient A is a compound of formula I. The active ingredient B is any one of ethomeburazole, lufenuron or flumiclorac. The mass ratio of the active ingredient A to the active ingredient B is 1:50-45:1. The acaricide has excellent control effect on common harmful mites on crops and garden plants, can effectively reduce the use amount of pesticides, and reduces the harm to the environment.

Description

[0001] This invention application is a divisional application of application number 202311368221.X, filed on October 23, 2023, entitled "An acaricide and its application". Technical Field

[0002] This invention belongs to the field of pesticide acaricide technology, and discloses an acaricide and its application. Background Technology

[0003] Etoxazole, CAS No.: 153233-91-1, chemical name: 4-(4-tert-butyl-2-ethoxyphenyl)-2-(2,6-difluorophenyl)-4,5-dihydro-1,3-oxazole, is an oxazole acaricide belonging to the diphenyloxazoline derivative class. It primarily controls mites by inhibiting their normal molting process and possesses ovicidal activity, thus effectively controlling mites throughout their entire larval stage and causing female adult mites to become sterile. It is mainly used to control red spider mites on apples and citrus fruits, and also shows excellent control efficacy against spider mites, including *Tetranychus simonii*, *Tetranychus pteropus*, *Tetranychus two-spotted*, and *Tetranychus carmine*, on cotton, flowers, and vegetables.

[0004] Lufenuron, CAS No.: 103055-07-8, chemical name: N-[[2,5-dichloro-4-(1,1,2,3,3,3-hexafluoropropoxy)phenyl]carbamoyl]-2,6-difluorobenzamide, is a chitin synthesis inhibitor. Its mechanism of action is to inhibit the enzyme that catalyzes chitin polymerization in mites / insects, thus interfering with the normal growth and development of insects.

[0005] Fluopyram, CAS No.: 918162-02-4, chemical name: (2E)-2-(2-{[2-chloro-4-(trifluoromethyl)phenoxy]methyl}phenyl)-3-methoxyacrylate methyl ester, is a highly effective fungicide and acaricide of the methoxyacrylate class. It can be used to control diseases caused by deuteromycetes, ascomycetes, basidiomycetes, and synmycetes, such as wheat rust, powdery mildew, cucumber powdery mildew, anthracnose, black spot, corn leaf blight, and rice sheath blight. It also has high activity against spider mites such as red spider mites on apples and citrus fruits.

[0006] Mites are a type of herbivorous arthropod, belonging to the phylum Arthropoda, class Arachnida, and order Acari. They are sparsely populated, have a wide range of activity, reproduce rapidly in hot and dry conditions, have short generation cycles, and exhibit strong resistance to pesticides. Agricultural mites are recognized worldwide as one of the most difficult biological groups to control. In my country, there are over 40 species of agricultural mites, which have a strong destructive ability towards fruit trees, vegetables, flowers, and other economic crops, severely affecting the normal physiological functions of crop leaves and even reducing yields. Currently, mite control mainly relies on chemical methods, with acaricides being the primary means of mite control in modern agriculture. However, in agricultural production, the efficacy of various acaricides against mites is decreasing year by year. The applicant's research has found that combining sulfiflumin with etoxazole, lufenuron, or flufenoxuron in appropriate mass ratios has a significant synergistic effect on mites, effectively improving the control effect of mites in the field, while also being safe for plants, reducing pesticide usage, and minimizing environmental pollution. Summary of the Invention

[0007] Based on the above, the purpose of this invention is to provide an acaricide that significantly enhances the efficacy of pesticides against harmful mites on agricultural and garden plants, thereby effectively reducing the amount of pesticides used and minimizing environmental harm.

[0008] To achieve the above objectives, the present invention adopts the following technical solution: an acaricide, wherein the acaricide comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: The active ingredient B is any one of etoxazole, lufenuron, or flufenoxuron;

[0009] Furthermore, the mass ratio of active ingredient A to active ingredient B in the acaricide is 1:50 to 45:1.

[0010] Furthermore, the mass ratio of active ingredient A to active ingredient B in the acaricide is 1:45 to 35:1;

[0011] Furthermore, the active ingredient B is etoxazole, and the mass ratio of active ingredient A to active ingredient B is 1:45 to 35:1;

[0012] The active ingredient B is lufenuron, and the mass ratio of active ingredient A to active ingredient B is 1:35 to 35:1;

[0013] The active ingredient B is fluopyram, and the mass ratio of active ingredient A to active ingredient B is 1:30 to 15:1.

[0014] Furthermore, based on the total weight of the acaricide as 100%, the total weight of active ingredient A and active ingredient B accounts for 0.1% to 80% of the total weight of the bactericidal composition;

[0015] Furthermore, in addition to the active ingredient, the acaricide also contains pesticide-permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

[0016] Furthermore, the acaricide can be prepared into a pesticide-acceptable formulation, which can be a solid or liquid formulation.

[0017] Further, the solid dosage form is granules, strips, wettable powder, oil-dispersible powder, emulsion powder, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powder, soluble tablets, or soluble granules; the liquid dosage form is soluble concentrate, soluble gel, oil, film-spreading oil, emulsion, latex, dispersible liquid, ointment, water emulsion, oil emulsion, microemulsion, lipid, suspension, microcapsule suspension, oil suspension, dispersible oil suspension, or suspension emulsion.

[0018] Furthermore, the solid dosage form is a wettable powder or a water-dispersible granule, and the liquid dosage form is an emulsifiable concentrate, a water suspension, or a water-dispersible granule;

[0019] This invention also discloses the application of the acaricide described above for the prevention and control of mites in crops and garden plants.

[0020] Furthermore, the plant mites mentioned are *Tetranychus carmineus*, *Tetranychus two-spotted*, and *Tetranychus citrus*.

[0021] The present invention has the following advantages:

[0022] The acaricide of this invention has a significant synergistic effect on common mites on agricultural and garden plants, especially effective against Tetranychus carmineus, Tetranychus two-spotted mites and citrus red spider mites. It can slow down the development of pesticide resistance in mites, reduce the amount of pesticides used, and is safe for plants and environmentally friendly. Detailed Implementation

[0023] To make the technical solution of the present invention clearer, the present invention will explain and illustrate the technical solution of the present invention in conjunction with specific preparation examples and embodiments.

[0024] Formulation preparation example:

[0025] Preparation Example 1: 27% sulfiflumin·etoxazole suspension (1:8)

[0026] Formula composition: 3% sulfiflumin, 24% etoxazole, 1% fatty alcohol polyoxyethylene ether, 5% styrene phenol polyoxyethylene ether phosphate, 1% sodium lignosulfonate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0027] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in the reaction vessel in sequence, water is added and mixed evenly, and then subjected to high-speed shearing, wet sand milling and finally homogenization filtration to obtain the suspension product.

[0028] Preparation Example 2: 42% sulfiflumin·etoxazole water-dispersible granules (5:1)

[0029] Formula composition: 35% sulfiflumin, 7% etoxazole, 8% sodium lignosulfonate, 2% BX (a type of styrene-based powder), 8% sodium polycarboxylate, 4% ammonium sulfate, and kaolin to make up the balance;

[0030] Preparation method: According to the formulation ratio in the example, add the active ingredient to the carrier, and add surfactants and other functional additives therein, mix, and after air jet pulverization, add 10-25% water, and then knead, granulate, dry and sieve to obtain water-dispersible granules; or spray water, granulate and dry the pulverized powder in a fluidized bed granulator, and then sieve to obtain the product.

[0031] Preparation Example 3: 16% sulfiflumin·etoxazole emulsifiable concentrate (1:3)

[0032] Formulation composition: 4% sulfiflumin, 12% etoxazole, 15% EO / PO block copolymer, 12% acetophenone, 10% propylene glycol methyl ether, 1% calcium dodecylbenzenesulfonate, xylene to make up the balance;

[0033] Preparation method: According to the formulation ratio in the example, the measured active ingredients, solvents and co-solvents are added to the mixing tank and stirred to dissolve them. Then, the emulsifier is added, and the remaining solvent is used to make up the balance. The mixture is stirred evenly in the mixing tank and filtered to obtain the emulsifiable oil required by the present invention.

[0034] Preparation Example 4: 12% sulfiflumin·lufenuron emulsifiable concentrate (1:2)

[0035] Formulation composition: 4% sulfiflumin, 8% lufenuron, 15% N-methylpyrrolidone, 14% styrene-phenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 10% DMF, methyl oleate to make up the balance;

[0036] Preparation method: Same as in preparation example 3.

[0037] Preparation Example 5: 21% sulfiflumin·lufenuron suspension (6:1)

[0038] Formula composition: 18% sulfiflumin, 3% lufenuron, 2% guerbert alcohol polyoxyethylene ether, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 2% tristyrene phenol ethoxylate phosphate, 1% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.2% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0039] Preparation method: Same as in preparation example 1.

[0040] Preparation Example 6: 30% sulfiflumin·lufenuron water-dispersible granules (3:2)

[0041] Formula composition: 18% sulfiflumin, 12% lufenuron, 10% dispersant NNO, 2% sodium dodecyl sulfate, 6% sodium lignosulfonate, 30% starch, and light calcium carbonate to make up the balance;

[0042] Preparation method: Same as in preparation example 2.

[0043] Preparation Example 7: 27% sulfiflumin·flufenicol suspension (4:5)

[0044] Formula composition: 12% sulfiflumin, 15% fluopyram, 2% isotridecyl alcohol polyoxyethylene ether, 4% tristyrylphenol ethoxylated phosphate, 1% lignosulfonate, 3% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 0.25% xanthan gum, 5% glycerol, 0.1% sodium benzoate, 0.5% silicone oil, deionized water to make up the balance;

[0045] Preparation method: Same as in preparation example 1.

[0046] Preparation Example 8: 39% sulfiflumin·flufenicol water-dispersible granules (1:12)

[0047] Formula composition: 3% sulfiflumin, 36% fluopyram, 8% sodium lignosulfonate, 2% bleaching powder BX, 8% naphthalene sulfonate formaldehyde condensate D425, 5% white sugar, kaolin to make up the balance;

[0048] Preparation method: Same as in preparation example 2.

[0049] Preparation Example 9: 16% sulfiflumin·flufenicol emulsifiable concentrate (7:1)

[0050] Formulation composition: 14% sulfiflumin, 2% fluopyram, 15% EO / PO block copolymer, 15% acetophenone, 10% N-octylpyrrolidone, 1% calcium dodecylbenzenesulfonate, and trimethylbenzene to make up the balance;

[0051] Preparation method: Same as in preparation example 3.

[0052] All the preparations prepared in the above examples have been tested and found to meet the quality and technical indicators required for the corresponding preparations. The preparations obtained are qualified preparations recognized in this field.

[0053] Example 1: Indoor Bioactivity Assay

[0054] Test basis: The test was conducted in accordance with NY / T 1154.12-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides - Part 12: Tetranychus Slide Immersion Method".

[0055] Experimental targets: Tetranychus urticae Koch and Tetranychus cinnabarinus, with nymphs in the same physiological state selected.

[0056] Test reagents: sulfiflumin technical grade, etoxazole technical grade, lufenuron technical grade, and flufenoxuron technical grade, provided by the group's R&D center.

[0057] Experimental preparation: Cut double-sided tape into 2cm lengths and attach them to one end of a glass slide. Select healthy mites and attach their backs to the double-sided tape (avoiding the legs, antennae, and mouthparts). Place 30 mites per slide and put them in a container lined with a damp sponge. Cover the container and incubate at 25±1℃. After 2 hours, examine under a microscope, remove any dead or injured individuals, and replenish the slide to 30 mites.

[0058] Reagent preparation: Dissolve the test reagent in a suitable solvent, and according to the purpose of mixing and the activity of the reagent, prepare 5 series of mass concentration gradients for each single agent and each group of mixed reagents.

[0059] Chemical treatment: Immerse the slide in the chemical solution and gently shake for 5 seconds. Remove the slide, absorb excess solution with filter paper, and place it in a white porcelain dish lined with a damp sponge. Cover with a transparent plastic film. Each treatment is repeated 4 times, and a blank control is set up with no chemical solution (containing all organic solvents and emulsifiers).

[0060] Feeding and observation: The containers containing the test insects were placed at a temperature of (25±1)℃ and a photoperiod of L:D=(16:8)h for feeding and observation.

[0061] Experimental investigation: Microscopic examination was performed 48 hours after treatment to check the mortality of the test insects, and the total number of insects and the number of dead insects were recorded.

[0062] Calculate the mortality rate for each treatment based on the survey data. Use the following formula:

[0063]

[0064] In the formula:

[0065] P – Mortality rate, expressed as a percentage (%);

[0066] K represents the number of dead insects, in heads;

[0067] N represents the total number of insects treated, in units of heads.

[0068]

[0069] In the formula:

[0070] P1 – Corrected mortality rate, in percentage (%);

[0071] P t —The mortality rate is expressed as a percentage (%).

[0072] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0073] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be made according to formula (2); if the control mortality rate is >20%, the experiment needs to be repeated.

[0074] Analyze using the DPS statistical analysis system to determine LC. 50 The value is used to evaluate the activity of the test reagent on the biological sample.

[0075] The co-toxicity coefficient (CTC value) of the mixture is calculated according to formulas (3), (4), and (5):

[0076]

[0077] In the formula:

[0078] ATI – Actual Measured Toxicity Index of Mixtures;

[0079] S – LC50 of standard acaricides 50 The unit is milligrams per liter (mg / L);

[0080] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0081] TTI = TI A ×P A +TI B ×P B

[0082] In the formula:

[0083] TTI – Theoretical Toxicity Index of Mixtures;

[0084] TI A—A. Toxicity index of drug A;

[0085] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0086] TI B —Toxicity index of drug B;

[0087] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0088]

[0089] In the formula:

[0090] CTC – Cotoxicity Coefficient;

[0091] ATI – Actual Measured Toxicity Index of Mixtures;

[0092] TTI – Theoretical Toxicity Index of Mixtures.

[0093] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0094] The results of the indoor toxicity tests are shown in the table below:

[0095] Table 1. Results of the indoor bioactivity assay of sulfiflumin and etoxazole mixture on Tetranychus bisporus.

[0096] Test reagents virulence regression equation <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Sulfiflumin(A) y = 5.4740 + 1.5962x 0.5047 / Ethoxydazole (B) y = 3.9338 + 1.5138x 5.0615 / A:B = 1:50 y = 4.0496 + 1.5161x 4.2352 101.535 A:B = 1:45 y = 4.2414 + 1.4151x 3.4358 123.146 A:B = 1:35 y = 4.2959 + 1.6452x 2.6789 151.055 A:B = 1:25 y = 4.4364 + 1.5463x 2.3144 162.327 A:B = 1:10 y = 4.6920 + 1.4960x 1.6065 173.037 A:B = 1:5 y = 4.9408 + 1.2350x 1.1167 180.955 A:B = 5:1 y = 5.8459 + 1.8599x 0.3509 169.221 A:B = 10:1 y = 5.8399 + 1.8213x 0.3458 158.961 A:B = 25:1 y = 5.6494 + 1.5785x 0.3878 134.812 A:B = 35:1 y = 5.5827 + 1.5207x 0.4138 125.096 A:B = 45:1 y = 5.5198 + 1.5875x 0.4705 109.410

[0097] Indoor biological experiments showed that a suitable mass ratio of sulfiflumin and etoxazole resulted in excellent control of the two-spotted spider mite. The mass ratio of sulfiflumin to etoxazole was 1:45–35:1, with a co-toxicity coefficient greater than 120, indicating a synergistic effect.

[0098] Table 2. Results of the indoor bioactivity assay of sulfiflumin and lufenuron-containing mixtures on Tetranychus bisaur.

[0099]

[0100]

[0101] Indoor testing results show that when sulfiflumin and lufenuron are combined in a mass ratio of 20:1 to 1:35, the co-toxicity coefficient against two-spotted spider mites is greater than 120, indicating a synergistic effect.

[0102] Table 3. Results of the indoor bioactivity assay of sulfiflumin and fluopyram mixtures on two-spotted spider mites.

[0103] Test reagents virulence regression equation <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Sulfiflumin(A) y = 5.4740 + 1.5962x 0.5047 / Fluopyram (B) y = 3.8921 + 1.7595x 4.2626 / A:B = 1:45 y = 4.2510 + 1.4573x 3.2658 112.339 A:B = 1:25 y = 4.3735 + 1.5011x 2.6142 126.756 A:B = 1:15 y = 4.5345 + 1.5005x 2.0428 142.398 A:B = 1:7 y = 4.7835 + 1.4748x 1.4022 157.450 A:B = 1:3 y = 5.0596 + 1.5926x 0.9174 162.379 A:B = 1:1 y = 5.3465 + 1.4701x 0.5811 155.315 A:B = 3:1 y = 5.5544 + 1.6337x 0.4578 141.412 A:B = 7:1 y = 5.5391 + 1.4118x 0.4151 136.643 A:B = 15:1 y = 5.4453 + 1.1766x 0.4183 127.691 A:B = 25:1 y = 5.3968 + 1.2876x 0.4918 106.225 A:B = 45:1 y = 5.2517 + 0.9649x 0.5484 93.830

[0104] Indoor biological test results show that sulfiflumin and fluopyram, when combined in a mass ratio of 1:25 to 15:1, exhibit a synergistic effect on two-spotted spider mites.

[0105] Table 4. Results of the indoor bioactivity assay of a mixture of sulfiflumin and etoxazole on Tetranychus cinnabarinus.

[0106] Test reagents virulence regression equation <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient Sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / Ethoxydazole (B) y = 3.7663 + 1.4953x 6.6842 / A:B = 1:50 y = 4.0485 + 1.4018x 4.7727 122.775 A:B = 1:45 y = 4.2043 + 1.3369x 3.9372 146.859 A:B = 1:35 y = 4.2168 + 1.3826x 3.6854 151.224 A:B = 1:25 y = 4.3367 + 1.3116x 3.2045 163.468 A:B = 1:8 y = 4.6126 + 1.3888x 1.9008 195.647 A:B = 1:5 y = 4.6697 + 1.4312x 1.7014 178.895 A:B = 5:1 y = 5.3287 + 1.4421x 0.5916 161.862 A:B = 8:1 y = 5.2649 + 1.1412x 0.5860 154.580 A:B = 25:1 y = 5.1907 + 1.1607x 0.6851 123.495 A:B = 35:1 y = 5.1921 + 1.3239x 0.7160 117.029 A:B = 45:1 y = 5.0652 + 1.0736x 0.8695 95.848

[0107] Indoor biological experiments showed that sulfiflumin and etoxazole, when combined at a mass ratio of 1:45 to 25:1, exhibited a synergistic effect against Tetranychus cinnabarinus.

[0108] Table 5. Results of the indoor bioactivity assay of sulfiflumin and lufenuron-containing mixtures on Tetranychus cinnabarinus.

[0109] Test reagents virulence regression equation <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / Lufenuron (B) y = 3.1179 + 1.6172x 14.5820 / A:B = 35:1 y = 5.2414 + 1.4879x 0.6883 121.969 A:B = 20:1 y = 5.3282 + 1.4803x 0.6002 142.615 A:B = 10:1 y = 5.4303 + 1.7083x 0.5599 159.714 A:B = 8:1 y = 5.3718 + 1.4601x 0.5563 164.172 A:B = 4:1 y = 5.4257 + 1.7504x 0.5712 176.427 A:B = 1:1 y = 5.0283 + 1.3805x 0.9538 162.320 A:B = 1:4 y = 4.5690 + 1.3264x 2.1132 157.996 A:B = 1:8 y = 4.1602 + 1.5130x 3.5897 141.499 A:B = 1:10 y = 4.1724 + 1.3251x 4.2127 136.780 A:B = 1:20 y = 3.8738 + 1.3203x 7.1290 113.524 A:B = 1:35 y = 3.7466 + 1.2794x 9.5425 104.116

[0110] Indoor biological experiments showed that sulfiflumin and lufenuron, when combined in a mass ratio of 1:10 to 35:1, exhibited a synergistic effect against Tetranychus cinnabarinus.

[0111] Table 6. Results of the indoor bioactivity assay of sulfiflumin and flufenoxuron-methyl mixtures on Tetranychus cinnabarin.

[0112] Test reagents virulence regression equation <![CDATA[LC 50 (mg / L)]]> Cotoxicity coefficient sulfiflumin(A) y = 5.1145 + 1.3081x 0.8175 / Fluopyram (B) y = 3.5223 + 1.7130x 7.2884 / A:B = 1:40 y = 3.8874 + 1.5416x 5.2694 115.933 A:B = 1:30 y = 4.0599 + 1.5073x 4.2047 138.082 A:B = 1:15 y = 4.3127 + 1.3569x 3.2101 151.899 A:B = 1:7 y = 4.6180 + 1.4351x 1.8456 198.502 A:B = 1:3 y = 4.7781 + 1.4987x 1.4062 173.994 A:B = 1:1 y = 5.0109 + 1.4576x 0.9829 149.568 A:B = 3:1 y = 5.1906 + 1.4383x 0.7370 142.567 A:B = 7:1 y = 5.2521 + 1.4608x 0.6721 136.818 A:B = 15:1 y = 5.2297 + 1.5358x 0.7087 122.129 A:B = 30:1 y = 5.1192 + 1.5014x 0.8330 101.033 A:B = 40:1 y = 5.0074 + 1.2794x 0.9867 84.686

[0113] Indoor biological test results show that sulfiflumin and fluopyram, when combined in a mass ratio of 1:30 to 15:1, exhibit a synergistic effect on Tetranychus cinnabarinus.

[0114] Example 2: Field efficacy test of the acaricide composition against two-spotted spider mites

[0115] Experimental site: This experiment was conducted in Shaojiazhuang Village, Mancheng District, Baoding City, Hebei Province. The soil fertility of the experimental site is high, the cultivation and management conditions are good, and the water and fertilizer management of all experimental plots is uniform and consistent, which is in line with local scientific agricultural practices.

[0116] Experimental target: Strawberry two-spotted spider mite.

[0117] Experimental crop: strawberry.

[0118] Experimental Design: The experiment consisted of 8 treatments, with water as a blank control. Each treatment was replicated 4 times, and each plot was 30m². 2 The randomized block arrangement was used, with each block space having two rows of strawberries between it.

[0119] Experimental method: A 3WBD-20 backpack electric sprayer was used to evenly spray the pesticide solution onto both sides of the strawberry leaves. The application was conducted on March 22, 2023, and was performed once.

[0120] Methods: The initial mite population was assessed before pesticide application. Population density was assessed on days 3 and 10 after application. A handheld magnifying glass was used to observe and record the number of live mites on both sides of the leaves. Five sampling points were used in each plot, with one plant sampled at each point and five mite-infested leaves sampled from each plant. The mite population was then assessed.

[0121] Methods for calculating drug efficacy:

[0122]

[0123] Results and Analysis:

[0124] Table 7. Field efficacy test results of the acaricide composition against two-spotted spider mite.

[0125]

[0126] Table 7 shows the field efficacy trials, indicating that the control efficacy of 27% sulfiflumin·etoxazole suspension (1:8), 21% sulfiflumin·lufenuron suspension (6:1), and 16% sulfiflumin·flufenoxam emulsifiable concentrate (7:1) against two-spotted spider mites was 85.53%, 81.00%, and 80.15% respectively 3 days after application, and 93.27%, 92.52%, and 89.44% respectively 14 days after application.

[0127] Example 3: Field efficacy test of the acaricide composition against Tetranychus carmineus

[0128] Experimental location: The experiment was conducted in a vegetable greenhouse in Shouguang City, Weifang City, Shandong Province. The soil layer was deep and the soil fertility was moderate to high. All experimental plots conformed to local conventional agricultural practices.

[0129] Test target: Tetranychus cinnabarinus.

[0130] Experimental crop: eggplant.

[0131] Experimental design: Each experimental plot has an area of ​​20m². 2All experimental plots were arranged in a randomized block design, with each treatment replicated four times. Application of pesticides was carried out at the early stage of Tetranychus carmine infestation. Each pesticide treatment was diluted to a specific concentration, and the entire eggplant plant was sprayed using a Gongnong-16 sprayer, ensuring even coverage on both sides of each leaf.

[0132] Survey Method: Five eggplant plants were fixed in each plot, and three leaves from each plant were surveyed. The initial population of spider mites was surveyed before pesticide application. The number of surviving mites was surveyed at 3, 7, and 15 days after pesticide application, and the mite population reduction rate and control effect were calculated.

[0133] Methods for calculating drug efficacy:

[0134]

[0135] Results and analysis of field efficacy trials:

[0136] Table 8. Field efficacy test results of the acaricide composition against Tetranychus cinnabarinus.

[0137]

[0138] Field efficacy trials showed that combining sulfiflumin with etoxazole, lufenuron, or flufenoxuron resulted in good control of Tetranychus carmine. 42% sulfiflumin·etoxazole water-dispersible granules (5:1), 30% sulfiflumin·lufenuron water-dispersible granules (3:2), and 27% sulfiflumin·flufenoxuron suspension (4:5) exhibited good rapid-acting effects against Tetranychus carmine 3 days after application. At 15 days post-application, the control efficacies of 42% sulfiflumin·etoxazole water-dispersible granules (5:1), 30% sulfiflumin·lufenuron water-dispersible granules (3:2), and 27% sulfiflumin·flufenoxuron suspension (4:5) against Tetranychus carmine were 93.59%, 95.64%, and 92.85%, respectively, with a relatively long residual effect.

[0139] Example 4: Field efficacy test of the acaricide composition against citrus red spider mite

[0140] Experimental location: Citrus orchard in Qingcao Village, Qingjiang Town, Zixing City, Hunan Province. The water and fertilizer conditions and cultivation management in this area are relatively consistent. The experimental crop is Dongjiang mandarin orange, which is growing normally.

[0141] Experimental Design: This experiment included 7 pesticide treatments and 1 water control. The pesticide treatments were: 16% sulfiflumin·etoxazole EC (1:3), 16% sulfiflumin·flufenoxam EC (7:1), 12% sulfiflumin·lufenuron EC (1:2), 20% etoxazole SC, 22% lufenuron EC, 21% flufenoxam SC, and 20% sulfiflumin water dispersible granules.

[0142] Experimental plot setup: 3 trees per treatment, replicated 4 times, randomly arranged. Protective rows were set up around the experimental plots; these protective rows were not treated with pesticides or water during the experiment and were managed using standard methods.

[0143] Application time: May 13, 2023. The weather was cloudy with a light breeze. Apply the pesticide by evenly spraying the leaves. When spraying, make sure that both the front and back of the leaves are evenly coated with the pesticide solution, starting from the beginning of dripping.

[0144] Survey methods: The initial mite population was surveyed before application of the pesticide, and the number of live mites was surveyed at 3, 8, and 14 days after application. For each plot, the tender shoots of each tree were marked in five directions: east, south, west, north, and center. The number of active mites on 25 leaves of each tree was surveyed. The leaf surface was directly observed with a handheld magnifying glass, and the number of mites was counted.

[0145] Methods for calculating drug efficacy:

[0146]

[0147] Results and analysis of field efficacy trials:

[0148] Table 9. Results of field efficacy tests of the acaricide composition against citrus red spider mites.

[0149]

[0150] Table 9 shows that the acaricide composition of the present invention has a good control effect on citrus red spider mites, and its rapid effect and long-lasting effect are significantly better than those of the single-agent control.

[0151] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. An acaricide, characterized in that, The acaricide comprises active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula I: (Formula I), wherein the active ingredient B is lufenuron, and the mass ratio of the active ingredient A to the active ingredient B is 1:35~20:

1.

2. The acaricide according to claim 1, wherein the mass ratio of active ingredient A to active ingredient B is 1:10 to 20:

1.

3. The acaricide according to claim 1, wherein the mass ratio of active ingredient A to active ingredient B is 1:35, 1:20, 1:10, 1:8, 1:4, 1:1, 4:1, 8:1, 10:1, or 20:

1.

4. The acaricide according to claim 1, characterized in that, Based on the total weight of the acaricide being 100wt%, the total weight of active ingredient A and active ingredient B accounts for 0.1wt% to 80wt% of the total weight of the acaricide.

5. The acaricide according to claim 1, characterized in that, In addition to the active ingredient, the acaricide contains pesticide-permitted auxiliary ingredients, which are selected from one or more of the following: wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, binders, fillers, or carriers.

6. The acaricide according to claim 1, characterized in that, The acaricide is prepared into a pesticide-acceptable formulation, which is either a solid or liquid formulation.

7. The acaricide according to claim 6, characterized in that, The solid formulation is a wettable powder or a water-dispersible granule, and the liquid formulation is an emulsifiable concentrate, a water emulsion, a microemulsion, a suspension concentrate, or a dispersible oil suspension.

8. The application of the acaricide according to any one of claims 1-7 for the control of mites in crops and garden plants, characterized in that, The plant pests mentioned are Tetranychus carmineus and Tetranychus sanguinea.

Images