Uniform streptavidin magnetic bead coating device and coating process

Abstract

The invention discloses a uniform streptavidin magnetic bead coating device and a coating process, and relates to the technical field of magnetic bead coating, the uniform streptavidin magnetic bead coating device comprises a shell, an ultrasonic transducer is arranged in the shell, a control panel is fixedly connected to the outer wall of one side of the shell, a coating box is fixedly connected to the interior of the shell, and the coating box is fixedly connected to the outer wall of the other side of the shell. A liquid inlet pipe and a liquid outlet pipe are arranged on the two sides of the shell respectively, an adsorption assembly used for improving coating uniformity, a first mixing assembly and a second mixing assembly are arranged in the shell, and the second mixing assembly is closer to the ultrasonic transducer than the first mixing assembly. According to the magnetic bead coating device, magnetic beads gathered on the side walls and the corners of the coating box can be adsorbed through the magnetic connecting belt and conveyed to a stirring area of the second mixing assembly, the magnetic beads are effectively prevented from being deposited on the edges and the corners of the coating box in the process, and the problem that the magnetic beads cannot be fully coated due to gathering is solved; and the magnetic bead coating uniformity of the whole device is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of magnetic bead coating, and more specifically, to a streptavidin magnetic bead uniform coating device and a coating process. Background Art

[0002] Streptavidin magnetic beads have a wide range of applications in the fields of biomedicine, molecular biology, etc., such as in experiments of nucleic acid extraction, protein purification, immunoassay, etc. Before using streptavidin magnetic beads, it is necessary to perform a coating treatment on them, that is, to uniformly fix specific biomolecules on the surface of the magnetic beads.

[0003] Currently, there are some deficiencies in the existing coating methods. For example, when manually operating, the coating is uneven, resulting in unstable performance of the magnetic beads and affecting the accuracy and repeatability of experimental results. Although some automated coating devices improve the coating efficiency, due to the easy attraction and aggregation between magnetic beads, during the coating process, the aggregated magnetic beads cannot be fully separated, which easily leads to uneven coating of some magnetic beads. At the same time, during the coating process, magnetic beads are prone to aggregation and precipitation at the corners or edges of the coating device, resulting in the magnetic beads in this area not being able to fully mix with the coating solution for coating work, thereby further affecting the coating uniformity. Therefore, there is an urgent need for a streptavidin magnetic bead uniform coating device and a coating process to solve the above problems. Summary of the Invention

[0004] In view of the problems in the related art, the present invention proposes a streptavidin magnetic bead uniform coating device and a coating process to overcome the above technical problems existing in the related art.

[0005] The technical solution of the present invention is realized as follows:

[0006] A streptavidin magnetic bead uniform coating device includes a housing. An ultrasonic transducer is arranged inside the housing. A control panel is fixedly connected to an outer wall of one side of the housing. A coating box is fixedly connected inside the housing. A liquid inlet pipe and a liquid discharge pipe are respectively arranged on both sides of the housing;

[0007] An adsorption component, a first mixing component, and a second mixing component for improving the coating uniformity are arranged inside the housing. The second mixing component is closer to the ultrasonic transducer than the first mixing component, and the first mixing component is arranged below the second mixing component;

[0008] The adsorption component includes a first gear arranged inside the coating box. A toothed belt is engaged with a circumferential outer wall of the first gear. A magnetic connection belt for adsorbing magnetic beads is fixedly connected to an outer wall of one side of the toothed belt;

[0009] A driving component for powering the adsorption component, the first mixing component, and the second mixing component is provided at the top of the coating box.

[0010] Further, the driving component includes a mounting plate fixedly connected to the outer wall of the top of the housing. A motor is fixedly connected to the outer wall of the top of the mounting plate. The output end of the motor is fixedly connected to a first rotating column. A third transmission wheel is fixedly connected to the circumferential outer wall of the first rotating column. The number of the third transmission wheels is two groups, and the two groups of third transmission wheels are distributed up and down. The circumferential outer wall of the third transmission wheel is connected to a second transmission belt in a transmission manner. The third transmission wheel is connected to a fourth transmission wheel through the second transmission belt. A fifth rotating column is fixedly connected to the circumferential outer wall of the fourth transmission wheel. The fifth rotating column is fixedly connected to the first gear. Both of the two second transmission belts are distributed in an isosceles triangle shape inside the coating box.

[0011] Further, a second vertical plate is fixedly connected to the inner wall of the bottom of the coating box. A second fixing frame is fixedly connected to the outer wall of the top of the second vertical plate. The second fixing frame is rotationally connected to the fifth rotating column.

[0012] Further, a first transmission wheel is fixedly connected to the circumferential outer wall of the first rotating column. The circumferential outer wall of the first transmission wheel is connected to a first transmission belt in a transmission manner. The first transmission wheel is connected to a second transmission wheel through the first transmission belt. A second rotating column is fixedly connected to the inner wall of the circumference of the second transmission wheel. A first fixing frame is fixedly connected to the outer wall of the top of the second fixing frame. The second rotating column is rotationally connected to the first fixing frame.

[0013] Further, a second gear is fixedly connected to the circumferential outer wall of the first rotating column. A reversing gear is meshed with the circumferential outer wall of the second gear. The reversing gear is meshed with another second gear. A fourth rotating column is fixedly connected to the inner wall of the circumference of the other second gear. A cross plate is fixedly connected to the outer wall of one side of the second vertical plate. The fourth rotating column is rotationally connected to the cross plate.

[0014] Further, the first mixing component includes a bearing seat fixedly connected to the circumferential outer wall of the fourth rotating column or the first rotating column. A bent column is fixedly connected to the circumferential outer wall of the bearing seat. A circular shell is fixedly connected to the end of the bent column away from the bearing seat. Equally spaced circularly distributed dispersion grooves are formed on the circumferential outer wall of the circular shell. A gear ring is fixedly connected to the circumferential inner wall of the dispersion groove. A third gear is meshed with the circumferential outer wall of the gear ring. The third gear is fixedly connected to the circumferential outer wall of the fourth rotating column or the first rotating column. A hub is fixedly connected to the circumferential outer wall of the first rotating column or the fourth rotating column. An impeller is arranged on the circumferential outer wall of the hub. The impeller is located inside the circular shell.

[0015] Further, the second mixing component includes a first vertical plate fixedly connected to the inner wall of the bottom of the coating box. A movable column is inserted into one side of the first vertical plate, and a spiral blade is fixedly connected to the circumferential outer wall of the movable column.

[0016] Further, limiting disks are fixedly connected to both ends of the movable column, and the diameter of the limiting disk is greater than the inner diameter of the movable column.

[0017] Further, a first cam is fixedly connected to the circumferential outer wall of the second rotating column. One end of the first cam is rotatably connected to a connecting rod, the other end of the connecting rod is rotatably connected to a second cam, a gear disk is fixedly connected to one side of the second cam, a toothed rod is fixedly connected to the circumferential outer wall of the movable column, the toothed rod meshes with the gear disk, a reinforcing plate is fixedly connected to the top outer wall of the first vertical plate, and a third rotating column is fixedly connected to the circumferential inner wall of the gear disk. The third rotating column is rotatably connected to the reinforcing plate.

[0018] A streptavidin magnetic bead uniform coating process, applied to the streptavidin magnetic bead uniform coating device described in the above embodiment, is characterized by including the following steps:

[0019] S1: Add the coating solution with a rated concentration into the coating box through the liquid inlet pipe. At the same time, add an appropriate amount of streptavidin magnetic beads into the coating box. Start the motor and the ultrasonic transducer through the control panel. The ultrasonic transducer generates ultrasonic waves to disperse the magnetic beads added into the coating box.

[0020] S2: Start the motor. The motor drives the first rotating column to rotate, and then drives the two third transmission wheels to rotate together. The third transmission wheel drives the fourth transmission wheel to rotate through the second transmission belt. When the fourth transmission wheel rotates, it drives the fifth rotating column and the first gear to rotate together. The first gear meshes with the toothed belt, driving the magnetic connection belt on the outer wall of the toothed belt to move in a circular rectangular path. The magnetic beads gathered on the side wall and corners of the coating box are adsorbed on its surface, and the magnetic beads are transported to the stirring area of the second mixing component to promote the preliminary mixing of the magnetic beads and the coating solution.

[0021] S3: Thoroughly mix the magnetic beads and the coating solution in the coating box through the first mixing component and the second mixing component for 40 minutes.

[0022] S4: After thorough mixing and coating, stop the operation of the motor and the ultrasonic transducer, open the drain pipe, and discharge the coated magnetic bead mixture.

[0023] The beneficial effects of the present invention:

[0024] A streptavidin magnetic bead uniform coating device and coating process provided by the present invention, through the provided first mixing component and second mixing component, when the magnetic beads are subjected to coating treatment, when the first rotating column and the fourth rotating column rotate, they drive the impeller to generate negative pressure, sucking the magnetic beads at the bottom of the coating box. At the same time, the third gear drives the gear ring to make the circular shell move in a circular motion, throwing the magnetic beads out of the dispersion tank, promoting the uniform distribution of the magnetic beads in the coating box, laying a good foundation for subsequent coating. During the process of the magnetic beads being fully mixed with the coating liquid through the first mixing component, the second rotating column drives the first cam, and through the connecting rod, the second cam rotates reciprocally, thereby driving the toothed rod, the movable column and the spiral blade to operate, generating a propelling force in the coating box, promoting the separation of the magnetic beads on the magnetic connection belt and participating in the mixing coating, significantly improving the coating quality.

[0025] A streptavidin magnetic bead uniform coating device and coating process provided by the present invention, through the provided adsorption component, the adsorption component drives the magnetic connection belt to perform a cyclic rectangular motion through the meshing of the first gear and the toothed belt. The magnetic connection belt can adsorb the magnetic beads aggregated on the side wall and corners of the coating box and transport them to the stirring area of the second mixing component. This process effectively avoids the deposition of magnetic beads at the edges and corners of the coating box, solves the problem that the magnetic beads cannot be fully coated due to aggregation, and improves the uniformity of the magnetic bead coating of the entire device.

[0026] A streptavidin magnetic bead uniform coating device and coating process provided by the present invention, the motor in the driving component drives the first rotating column, and then the two third driving wheels rotate. The fourth driving wheel is driven through the second transmission belt, driving the fifth rotating column and the first gear to operate. At the same time, the two second transmission belts are distributed in an isosceles triangle in the coating box. When stirring the coating liquid, the motion states of the belts on each side are different, forming a unique composite flow field. This flow field breaks the magnetic bead aggregates at the apex of the triangle, driving the coating liquid to form a complex circulation and cross flow in the chamber, efficiently covering the reaction chamber space, reducing local energy concentration, reducing the risk of magnetic bead damage, and comprehensively improving the magnetic bead coating effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the following described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0028] Figure 1 It is a schematic front view of the whole of the present invention.

[0029] Figure 2 For the present invention Figure 1 The enlarged schematic structure diagram at A in.

[0030] Figure 3 This is the overall top view structural schematic diagram of the present invention.

[0031] Figure 4 For the present invention Figure 3 The enlarged structural schematic diagram at position B in it.

[0032] Figure 5 This is the overall half-sectional view structural schematic diagram of the present invention.

[0033] Figure 6 For the present invention Figure 5 The enlarged structural schematic diagram at position C in it.

[0034] Figure 7 This is the front view after the internal structure of the coating box of the present invention is disassembled.

[0035] Figure 8 This is the rear view after the internal structure of the coating box of the present invention is disassembled.

[0036] Figure 9 For the present invention Figure 8 The enlarged structural schematic diagram at position D in it.

[0037] In the figure:

[0038] 1. Housing; 2. Coating box; 3. Liquid inlet pipe; 4. Mounting plate; 5. Motor; 6. Magnetic connection belt; 7. Control panel; 8. Drain pipe; 9. First rotating column; 10. First transmission wheel; 11. First transmission belt; 12. Tooth belt; 13. First fixed frame; 14. First cam; 15. Second transmission wheel; 16. Second rotating column; 17. Second fixed frame; 18. Second cam; 19. Link; 20. First gear; 21. Reversing gear; 22. Second gear; 23. Movable column; 24. First vertical plate; 25. Spiral blade; 26. Cross plate; 27. Tooth bar; 28. Gear disc; 29. Reinforcing plate; 30. Second transmission belt; 31. Third rotating column; 32. Fourth rotating column; 33. Ultrasonic transducer; 34. Circular shell; 35. Dispersion tank; 36. Third transmission wheel; 37. Bent column; 38. Bearing seat; 39. Gear ring; 40. Third gear; 41. Impeller; 42. Hub; 43. Second vertical plate; 44. Fifth rotating column; 45. Fourth transmission wheel. Specific embodiments

[0039] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art belong to the scope of protection of the present invention.

[0040] Please refer toFigures 1-9 , a streptavidin magnetic bead uniform coating device, comprising a housing 1. An ultrasonic transducer 33 is arranged inside the housing 1. A control panel 7 is fixedly connected to an outer wall of one side of the housing 1. A coating box 2 is fixedly connected inside the housing 1. A liquid inlet pipe 3 and a liquid discharge pipe 8 are respectively arranged on two sides of the housing 1;

[0041] An adsorption component, a first mixing component and a second mixing component for improving coating uniformity are arranged inside the housing 1. The second mixing component is closer to the ultrasonic transducer 33 than the first mixing component. The first mixing component is arranged below the second mixing component;

[0042] The adsorption component includes a first gear 20 arranged inside the coating box 2. A toothed belt 12 is meshed with the circumferential outer wall of the first gear 20. A magnetic connection belt 6 for adsorbing magnetic beads is fixedly connected to an outer wall of one side of the toothed belt 12;

[0043] A driving component for providing power to the adsorption component, the first mixing component and the second mixing component is arranged at the top of the coating box 2. When streptavidin magnetic beads are coated, the coating liquid is added into the coating box 2 inside the housing 1 through the liquid inlet pipe 3, and magnetic beads are put in at the same time. The ultrasonic transducer 33 is started to preliminarily disperse the magnetic beads to prevent them from aggregating. At the same time, the first gear 20 in the adsorption component is meshed with the toothed belt 12. The magnetic connection belt 6 on the toothed belt 12 makes a circular rectangular path movement driven by a motor 5, can adsorb the magnetic beads aggregated on the side wall and corners of the coating box 2, and convey them to the vicinity of the second mixing component. Through the collaborative work of the first mixing component and the second mixing component, the magnetic beads and the coating liquid are fully mixed to improve the coating uniformity. The driving component provides power to the adsorption component, the first mixing component and the second mixing component to ensure the normal operation of each component.

[0044] Preferably, the driving assembly includes a mounting plate 4 fixedly connected to the outer wall of the top of the housing 1. A motor 5 is fixedly connected to the outer wall of the top of the mounting plate 4. The output end of the motor 5 is fixedly connected to a first rotating column 9. A third transmission wheel 36 is fixedly connected to the circumferential outer wall of the first rotating column 9. The number of the third transmission wheels 36 is two groups, and the two groups of third transmission wheels 36 are distributed up and down. The circumferential outer wall of the third transmission wheel 36 is drivingly connected to a second transmission belt 30. The third transmission wheel 36 is drivingly connected to a fourth transmission wheel 45 through the second transmission belt 30. A fifth rotating column 44 is fixedly connected to the circumferential outer wall of the fourth transmission wheel 45. The fifth rotating column 44 is fixedly connected to a first gear 20. The two second transmission belts 30 are both distributed in an isosceles triangle in the coating box 2. When stirring the coating liquid, the motion states of the belts on each side are different, forming a unique composite flow field. This flow field breaks the magnetic bead aggregates at the apex of the triangle, drives the coating liquid to form a complex circulation and cross flow in the chamber, efficiently covers the space of the reaction chamber, reduces local energy concentration, reduces the risk of magnetic bead damage, and comprehensively improves the magnetic bead coating effect.

[0045] Preferably, a second vertical plate 43 is fixedly connected to the inner wall of the bottom of the coating box 2. A second fixing frame 17 is fixedly connected to the outer wall of the top of the second vertical plate 43. The second fixing frame 17 is rotationally connected to the fifth rotating column 44. The second fixing frame 17 at the top of the second vertical plate 43 is rotationally connected to the fifth rotating column 44, providing support for the fifth rotating column 44 to ensure its stable rotation, and thus ensuring the normal operation of the adsorption assembly.

[0046] Preferably, a first transmission wheel 10 is fixedly connected to the circumferential outer wall of the first rotating column 9. The circumferential outer wall of the first transmission wheel 10 is drivingly connected to a first transmission belt 11. The first transmission wheel 10 is drivingly connected to a second transmission wheel 15 through the first transmission belt 11. A second rotating column 16 is fixedly connected to the inner wall of the circumferential of the second transmission wheel 15. A first fixing frame 13 is fixedly connected to the outer wall of the top of the second fixing frame 17. The second rotating column 16 is rotationally connected to the first fixing frame 13, enabling the power of the first rotating column 9 to be transmitted to the second rotating column 16, providing a power source for the operation of subsequent related components.

[0047] Preferably, a second gear 22 is fixedly connected to the circumferential outer wall of the first rotating column 9. A reversing gear 21 is meshed with the circumferential outer wall of the second gear 22. The reversing gear 21 is meshed with another second gear 22. A fourth rotating column 32 is fixedly connected to the inner wall of the circumferential of the other second gear 22. A cross plate 26 is fixedly connected to the outer wall of one side of the second vertical plate 43. The fourth rotating column 32 is rotationally connected to the cross plate 26, which can make the fourth rotating column 32 rotate in the same direction as the first rotating column 9, providing power for the operation of the first mixing assembly.

[0048] Preferably, the first mixing component includes a bearing seat 38 fixedly connected to the circumferential outer wall of the fourth rotating column 32 or the first rotating column 9. A bent column 37 is fixedly connected to the circumferential outer wall of the bearing seat 38. One end of the bent column 37 away from the bearing seat 38 is fixedly connected to a circular shell 34. The circumferential outer wall of the circular shell 34 is provided with evenly spaced and circularly distributed dispersion grooves 35. The circumferential inner wall of the dispersion grooves 35 is fixedly connected to a gear ring 39. A third gear 40 meshes with the circumferential outer wall of the gear ring 39. The third gear 40 is fixedly connected to the circumferential outer wall of the fourth rotating column 32 or the first rotating column 9. A hub 42 is fixedly connected to the circumferential outer wall of the first rotating column 9 or the fourth rotating column 32. An impeller 41 is arranged on the circumferential outer wall of the hub 42. The impeller 41 is located inside the circular shell 34. When the first rotating column 9 or the fourth rotating column 32 rotates, the impeller 41 generates negative pressure to suck the magnetic beads at the bottom of the coating box 2, and at the same time, the third gear 40 drives the gear ring 39 to make the circular shell 34 move circumferentially, throwing the magnetic beads out of the dispersion grooves 35, promoting the uniform distribution of the magnetic beads in the coating box 2 and laying a good foundation for subsequent coating.

[0049] Preferably, the second mixing component includes a first vertical plate 24 fixedly connected to the inner wall of the bottom of the coating box 2. A movable column 23 is inserted on one side of the first vertical plate 24. A spiral blade 25 is fixedly connected to the circumferential outer wall of the movable column 23. When the movable column 23 rotates, the spiral blade 25 can stir and mix the magnetic beads and the coating liquid in the coating box 2, prompting the separation of the magnetic beads on the magnetic connection belt 6 and participating in the mixed coating.

[0050] Preferably, limit disks are fixedly connected to both ends of the movable column 23. The diameter of the limit disks is larger than the inner diameter of the movable column 23, which can prevent the movable column 23 from falling off the first vertical plate 24 during the movement process and ensure the stability of the operation of the second mixing component.

[0051] Preferably, a first cam 14 is fixedly connected to the circumferential outer wall of the second rotating column 16. One end of the first cam 14 is rotatably connected to a connecting rod 19. The other end of the connecting rod 19 is rotatably connected to a second cam 18. A gear disk 28 is fixedly connected to one side of the second cam 18. A toothed rod 27 is fixedly connected to the circumferential outer wall of the movable column 23. The toothed rod 27 meshes with the gear disk 28. A reinforcing plate 29 is fixedly connected to the top outer wall of the first vertical plate 24. A third rotating column 31 is fixedly connected to the circumferential inner wall of the gear disk 28. The third rotating column 31 is rotatably connected to the reinforcing plate 29. When the second rotating column 16 rotates, it drives the first cam 14, and through the connecting rod 19, the second cam 18 reciprocally rotates, thereby driving the toothed rod 27, the movable column 23 and the spiral blade 25 to operate, generating a propelling force in the coating box 2, prompting the separation of the magnetic beads on the magnetic connection belt 6 and participating in the mixed coating, and significantly improving the coating quality.

[0052] A streptavidin magnetic bead uniform coating process, which is applied to a streptavidin magnetic bead uniform coating device in the above embodiment, is characterized by including the following steps:

[0053] Step 1: Add the coating solution with a rated concentration into the coating box 2 through the liquid inlet pipe 3. At the same time, add an appropriate amount of streptavidin magnetic beads into the coating box 2. Start the motor 5 and the ultrasonic transducer 33 through the control panel 7. The ultrasonic transducer 33 generates ultrasonic waves to disperse the magnetic beads added into the coating box 2.

[0054] Step 2: Start the motor 5. The motor 5 drives the first rotating column 9 to rotate, and then drives the two third transmission wheels 36 to rotate together. The third transmission wheel 36 drives the fourth transmission wheel 45 to rotate through the second transmission belt 30. When the fourth transmission wheel 45 rotates, it drives the fifth rotating column 44 and the first gear 20 to rotate together. The first gear 20 meshes with the toothed belt 12, driving the magnetic connection belt 6 on the outer wall of the toothed belt 12 to move in a circular rectangular path. The magnetic beads aggregated on the side wall and corners of the coating box 2 are adsorbed on its surface through the magnetic connection belt 6, and the magnetic beads are transported to the stirring area of the second mixing component to promote the preliminary mixing of the magnetic beads and the coating solution.

[0055] Step 3: The first mixing component and the second mixing component are used to fully mix the magnetic beads and the coating solution in the coating box 2 for 40 minutes.

[0056] Step 4: After sufficient mixing and coating, stop the operation of the motor 5 and the ultrasonic transducer 33, open the drain pipe 8, and discharge the coated magnetic bead mixture.

[0057] In summary, by means of the above technical solution of the present invention, during use, the staff adds the rated concentration into the coating box 2, and at the same time adds an appropriate amount of magnetic beads into the coating box 2 and starts the motor 5 and the ultrasonic transducer 33. At this time, ultrasonic waves are generated by the ultrasonic generator to disperse the magnetic beads added into the coating box 2, preventing the magnetic beads from aggregating before coating, ensuring that the magnetic beads can be evenly suspended in the solution, and providing a good basis for subsequent uniform coating. At the same time, the motor 5 can drive the first rotating column 9 to rotate. During the rotation of the first rotating column 9, the two groups of third transmission wheels 36 can be driven to rotate together. At this time, the third transmission wheel 36 can make the fourth transmission wheel 45 rotate through the third transmission belt. When the fourth transmission wheel 45 rotates, it can drive the fifth rotating column 44 and the first gear 20 to rotate together. Through the meshing between the first gear 20 and the toothed belt 12, multiple magnetic connection belts 6 on the outer wall of the toothed belt 12 can be driven to move in a circular rectangular path. During this process, the magnetic beads aggregated on the side wall and corners of the coating box 2 can be adsorbed on the surface of the magnetic connection belt 6 through the magnetic connection belt 6, and at the same time, the magnetic beads are transported to the stirring area of the second mixing assembly through the magnetic connection belt 6 for subsequent mixing and coating work, avoiding the deposition of more uncoated magnetic beads on the side wall and corners of the coating box 2, improving the uniformity of the magnetic bead coating of the entire device, and during the circular motion of the magnetic connection belt 6, the third transmission belt, etc., the inside of the coating box 2 can be circularly stirred. Moreover, the third transmission belt is distributed in an isosceles triangle in the coating box 2. When the transmission belt distributed in an isosceles triangle is used for coating liquid stirring, a unique composite flow field can be generated. Because the moving directions and speeds of the transmission belts on each side are different, the coating liquid forms a complex circulation and cross flow in the triangular area, and the magnetic bead aggregates can be broken at the top angle, promoting the mixing of the magnetic beads and the coating liquid. At the same time, this layout can more efficiently cover the reaction chamber space with a smaller transmission belt length, drive the flow of the coating liquid in the areas prone to flow dead corners such as the corners, avoid the aggregation of magnetic beads, and in addition, it can reduce the excessive concentration of local energy, reduce the risk of magnetic bead damage, ensure the stirring efficiency, and improve the coating effect of the magnetic beads;

[0058] At the same time, during the rotation of the first rotating column 9, the second gear 22 can be driven to rotate together. Through the transmission between the second gear 22 and the reversing gear 21, multiple groups of fourth rotating columns 32 can be driven to rotate in the same direction as the first rotating column 9. When the first rotating column 9 and the fourth rotating column 32 rotate, the impeller 41 can be driven to rotate to generate negative pressure suction inside the coating box 2, so as to suck the magnetic beads deposited at the bottom of the coating box 2 into the coating box 2. At the same time, the third gear 40 will drive the gear ring 39 to rotate together, so as to drive the entire circular shell 34 to make a circular motion. Through the cooperation of the circular shell 34 and the impeller 41, the sucked magnetic beads can be quickly thrown out from the dispersion tank 35, so that the magnetic beads are evenly dispersed again inside the coating box 2, which is beneficial to the subsequent circular and uniform coating work of the magnetic beads;

[0059] During the rotation of the first rotating column 9, it can drive the first transmission wheel 10 to rotate together. Through the mutual cooperation between the first transmission wheel 10 and the first transmission belt 11, it can drive the second transmission wheel 15 and the second rotating column 16 to rotate together. When the second rotating column 16 rotates, it can drive the first cam 14 to rotate together. When the first cam 14 rotates, it can drive the second cam 18 to reciprocate through the connecting rod 19. During the reciprocating rotation of the second cam 18, it can drive the rack 27 to reciprocate and move horizontally at the same time. During the rotation of the rack 27, it can drive the movable column 23 and the spiral blade 25 to rotate together, so as to generate a forward or reverse pushing force inside the coating box 2. This pushing force acts on the magnetic beads adsorbed on the magnetic connection belt 6, so as to separate the magnetic beads from the magnetic connection belt 6 for subsequent mixing and coating work, improving the coating quality of the magnetic beads.

[0060] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A streptavidin magnetic bead uniform coating device, comprising a housing (1), characterized in that: An ultrasonic transducer (33) is arranged inside the shell (1), a control panel (7) is fixedly connected to an outer wall of one side of the shell (1), a coating box (2) is fixedly connected inside the shell (1), and a liquid inlet pipe (3) and a liquid outlet pipe (8) are respectively arranged on both sides of the shell (1); The housing (1) is provided with an adsorption component for improving coating uniformity, a first mixing component and a second mixing component, the second mixing component being closer to the ultrasonic transducer (33) than the first mixing component, and the first mixing component being provided below the second mixing component; The adsorption assembly comprises a first gear (20) arranged inside the coating box (2), a toothed belt (12) meshing on the circumferential outer wall of the first gear (20), and a magnetic connection belt (6) for adsorbing magnetic beads is fixedly connected to one side outer wall of the toothed belt (12); A driving assembly for providing power to the adsorption assembly, the first mixing assembly and the second mixing assembly is arranged on the top of the coating box (2).

2. The streptavidin magnetic bead uniform coating device according to claim 1, characterized in that: The driving assembly comprises a mounting plate (4) fixedly connected to the top outer wall of the shell (1); the top outer wall of the mounting plate (4) is fixedly connected to a motor (5); the output end of the motor (5) is fixedly connected to a first rotating column (9); the circumferential outer wall of the first rotating column (9) is fixedly connected to a third transmission wheel (36); the number of the third transmission wheels (36) is two groups, and the two groups of the third transmission wheels (36) are distributed up and down; the circumferential outer wall of the third transmission wheel (36) is transmission-connected to a second transmission belt (30); the third transmission wheel (36) is transmission-connected to a fourth transmission wheel (45) through the second transmission belt (30); the circumferential outer wall of the fourth transmission wheel (45) is fixedly connected to a fifth rotating column (44); the fifth rotating column (44) is fixedly connected to the first gear (20); and the two groups of the second transmission belts (30) are distributed in the form of an isosceles triangle in the bag box (2).

3. The streptavidin magnetic bead uniform coating device according to claim 2, characterized in that: The bottom inner wall of the packaging box (2) is fixedly connected to a second vertical plate (43), the top outer wall of the second vertical plate (43) is fixedly connected to a second fixed frame (17), and the second fixed frame (17) is rotatably connected to the fifth rotating column (44).

4. The streptavidin magnetic bead uniform coating device according to claim 3, characterized in that: The circumferential outer wall of the first rotating column (9) is fixedly connected to a first transmission wheel (10), the circumferential outer wall of the first transmission wheel (10) is transmission-connected to a first transmission belt (11), the first transmission wheel (10) is transmission-connected to a second transmission wheel (15) via the first transmission belt (11), the circumferential inner wall of the second transmission wheel (15) is fixedly connected to a second rotating column (16), the top outer wall of the second fixed frame (17) is fixedly connected to the first fixed frame (13), and the second rotating column (16) is rotationally connected to the first fixed frame (13).

5. The streptavidin magnetic bead uniform coating device according to claim 4, characterized in that: The circumferential outer wall of the first rotating column (9) is fixedly connected to a second gear (22), the circumferential outer wall of the second gear (22) is meshed with a reversing gear (21), the reversing gear (21) is meshed with another second gear (22), the circumferential inner wall of the other second gear (22) is fixedly connected to a fourth rotating column (32), the outer wall of one side of the second vertical plate (43) is fixedly connected to a horizontal plate (26), and the fourth rotating column (32) is rotatably connected to the horizontal plate (26).

6. The streptavidin magnetic bead uniform coating device according to claim 5, characterized in that: The first mixing assembly comprises a bearing seat (38) fixedly connected to the circumferential outer wall of the fourth rotating column (32) or the first rotating column (9); the circumferential outer wall of the bearing seat (38) is fixedly connected to a bent column (37); one end of the bent column (37) away from the bearing seat (38) is fixedly connected to a round shell (34); the circumferential outer wall of the round shell (34) is provided with dispersion grooves (35) distributed in a circular shape at equal distances; the circumferential inner wall of the dispersion grooves (35) is fixedly connected to A gear ring (39) is provided, and a third gear (40) is meshed on the circumferential outer wall of the gear ring (39), and the third gear (40) is fixedly connected to the circumferential outer wall of the fourth rotating column (32) or the first rotating column (9), and a hub (42) is fixedly connected to the circumferential outer wall of the first rotating column (9) or the fourth rotating column (32), and an impeller (41) is provided on the circumferential outer wall of the hub (42), and the impeller (41) is located inside the circular shell (34).

7. The streptavidin magnetic bead uniform coating device according to claim 6, characterized in that: The second mixing assembly comprises a first vertical plate (24) fixedly connected to the inner wall of the bottom of the coating box (2), a movable column (23) is inserted into one side of the first vertical plate (24), and a spiral blade (25) is fixedly connected to the circumferential outer wall of the movable column (23).

8. The streptavidin magnetic bead uniform coating device according to claim 7, characterized in that: Both ends of the movable column (23) are fixedly connected to a limiting disk, and the diameter of the limiting disk is larger than the inner diameter of the movable column (23).

9. The streptavidin magnetic bead uniform coating device according to claim 8, characterized in that: The circumferential outer wall of the second rotating column (16) is fixedly connected to the first cam (14), one end of the first cam (14) is rotatably connected to a connecting rod (19), the other end of the connecting rod (19) is rotatably connected to the second cam (18), one side of the second cam (18) is fixedly connected to a gear plate (28), the circumferential outer wall of the movable column (23) is fixedly connected to a gear rod (27), the gear rod (27) is meshed with the gear plate (28), the top outer wall of the first vertical plate (24) is fixedly connected to a reinforcing plate (29), the circumferential inner wall of the gear plate (28) is fixedly connected to a third rotating column (31), and the third rotating column (31) is rotatably connected to the reinforcing plate (29).

10. A streptavidin magnetic bead uniform coating process, applied to a streptavidin magnetic bead uniform coating device as claimed in claim 9, characterized in that: The following steps are involved: S1: Add a coating solution of a rated concentration into the coating box (2) through a liquid inlet tube (3), and simultaneously add an appropriate amount of streptavidin magnetic beads into the coating box (2), and start the motor (5) and the ultrasonic transducer (33) through a control panel (7), so that the ultrasonic transducer (33) generates ultrasonic waves to disperse the magnetic beads added into the coating box (2); S2: starting the motor (5), the motor (5) drives the first rotating column (9) to rotate, and then drives the two sets of third transmission wheels (36) to rotate together, the third transmission wheel (36) drives the fourth transmission wheel (45) to rotate through the second transmission belt (30), when the fourth transmission wheel (45) rotates, it drives the fifth rotating column (44) and the first gear (20) to rotate together, the first gear (20) and the toothed belt (12) are meshed with each other, driving the magnetic connection belt (6) on the outer wall of the toothed belt (12) to move in a circular rectangular path, the magnetic beads gathered on the side wall and corner of the coating box (2) are adsorbed on its surface by the magnetic connection belt (6), and the magnetic beads are transported to the stirring area of ​​the second mixing component, so as to promote the initial mixing of the magnetic beads and the coating liquid; S3: fully mixing the magnetic beads and the coating solution in the coating box (2) by the first mixing component and the second mixing component for 40 minutes; S4: After the beads are fully mixed and coated, the motor (5) and the ultrasonic transducer (33) are stopped, and the drain pipe (8) is opened to discharge the coated magnetic bead mixture.

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