Liquid changing assembly and liquid changing method

Abstract

The invention discloses a liquid changing assembly and a liquid changing method.The liquid changing assembly is used for distributing liquid to a reaction face of a sample carrier, the liquid changing assembly comprises a coating tool bit, the coating tool bit comprises a first block and a second block, the first block comprises a first side wall, the first side wall is provided with a first groove, and the second block is provided with a second groove; at least one of the first block body and the second block body is provided with a liquid inlet channel communicated with the first groove. The first block body comprises a first side wall, the second block body comprises a second side wall embedded with the first side wall, a liquid outlet channel is formed between the first groove and the second block body, a liquid outlet is formed between the first groove and the second block body, the first block body or the second block body is further provided with an emptying channel, and the emptying channel is communicated with the first groove; therefore, the previous liquid left in the coating tool bit can be discharged through the emptying channel, so that the risk of liquid pollution caused by liquid change can be reduced, and the accuracy of a biochemical reaction result can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biochemical equipment, and particularly relates to a liquid changing component and a liquid changing method. Background Art

[0002] In the related art, a sample carrier includes a reaction surface, and a corresponding biochemical reaction is completed by wetting the reaction surface with a reagent reaction solution. In order to reduce the amount of the reaction solution used, in an existing liquid distribution method, a coating cutter head is directly used to distribute the reaction solution onto the reaction surface. The coating cutter head includes two blocks. After the two blocks are joined together, the liquid outlet channel of the coating cutter head is located between the two blocks. However, in the above structure, after using the coating cutter head to distribute one reaction solution onto the reaction surface of the sample carrier, there is still some reaction solution remaining in the coating cutter head. When it is necessary to use the coating cutter head to distribute another reaction solution onto the reaction surface of the sample carrier, the previous reaction solution remaining in the coating cutter head will contaminate the current reaction solution, affecting the accuracy of the biochemical reaction result. Summary of the Invention

[0003] The present invention aims to at least solve one of the technical problems existing in the prior art. For this purpose, the present invention provides a liquid changing component and a liquid changing method, which can reduce the risk of liquid contamination caused by liquid changing.

[0004] The liquid changing component according to the first aspect embodiment of the present invention is used to distribute liquid onto the reaction surface of a sample carrier. The liquid changing component includes a coating cutter head, and the coating cutter head includes:

[0005] A first block including a first side wall, and a first groove is provided on the first side wall, and the first groove extends along a first direction to the side of the first side wall; and,

[0006] A second block including a second side wall that fits with the first side wall, an outlet channel is formed between the first groove and the second block, and an outlet is formed between the port of the first groove extending to the side and the second block;

[0007] At least one of the first block and the second block is provided with an inlet channel communicating with the first groove, and the inlet channel is in communication with the liquid;

[0008] Wherein, the first block or the second block is further provided with a drainage channel communicating with the first groove;

[0009] The liquid changing component further includes a first driver and a second driver. The first driver is used to drive the liquid to the inlet channel and make the liquid flow out of the outlet after passing through the outlet channel; the second driver is used to drive the liquid in the first groove to be discharged from the coating cutter head through the drainage channel.

[0010] The liquid changing assembly according to the embodiment of the first aspect of the present invention has at least the following beneficial effects:

[0011] The coating blade head is provided with an evacuation channel in the first block or the second block, and the evacuation channel communicates with the first groove. After using the coating blade head to arrange a liquid on the reaction surface of the sample carrier, when it is necessary to use the coating blade head to arrange another liquid on the reaction surface of the sample carrier, the residual previous liquid in the coating blade head can be discharged to the outside through the evacuation channel, thereby reducing the risk of liquid contamination caused by liquid change and being beneficial to improving the accuracy of the biochemical reaction result.

[0012] According to some embodiments of the present invention, the first side wall is further provided with a liquid guiding groove, the liquid guiding groove communicates with a side of the first groove away from the liquid outlet, the depth of the liquid guiding groove is greater than the depth of the first groove, the liquid inlet channel communicates with one end of the liquid guiding groove along the length direction, and the evacuation channel communicates with the other end of the liquid guiding groove along the length direction.

[0013] According to some embodiments of the present invention, the evacuation channel is inclined upward in a direction away from the liquid guiding groove.

[0014] According to some embodiments of the present invention, the first side wall is provided with a second groove, the second groove is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove, and a first liquid isolation cavity is formed between the second groove and the second block; alternatively, the second side wall is provided with a second groove, the second groove is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove, and a first liquid isolation cavity is formed between the second groove and the first block.

[0015] According to some embodiments of the present invention, the first side wall is provided with the second groove, the second groove is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove, and the first liquid isolation cavity is formed between the second groove and the second block;

[0016] The second side wall is provided with a third groove, the third groove is arranged on a side of the first groove away from the liquid outlet, and a second liquid isolation cavity is formed between the third groove and the first block;

[0017] The first liquid isolation cavity and the second liquid isolation cavity are arranged at intervals or at least partially overlap.

[0018] According to some embodiments of the present invention, a fourth groove is arranged at an end of the coating blade head along the length direction, and the fourth groove communicates with the second groove and / or the third groove.

[0019] According to some embodiments of the present invention, a recess is provided at the lower end of the coating blade head, and the recess is located between the liquid outlet and the fourth groove.

[0020] According to some embodiments of the present invention, the coating blade head has a hydrophobic coating, and the hydrophobic coating is provided on the outer side wall of the liquid outlet.

[0021] According to some embodiments of the present invention, it further includes a drying assembly movably arranged relative to the coating blade head, and the drying assembly includes a gas outlet for spraying gas toward the liquid outlet to dry the liquid outlet.

[0022] According to some embodiments of the present invention, the liquid changing assembly further includes a sensor for detecting bubbles, the sensor is arranged in the first groove, and the sensor is communicatively connected with the second driver.

[0023] A liquid changing method using the above liquid changing assembly according to the second aspect embodiments of the present invention includes:

[0024] The first liquid adding step: driving the first driver to introduce a first reagent into the coating blade head through the liquid inlet channel and distributing the first reagent to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet;

[0025] The liquid changing and cleaning step: driving the first driver to introduce a cleaning reagent into the coating blade head, and driving the second driver to discharge the first reagent and the cleaning reagent in the first groove out through the emptying channel; and

[0026] The second liquid adding step: closing the second driver, and introducing a second reagent into the coating blade head through the first driver through the liquid inlet channel and distributing the second reagent to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet.

[0027] The liquid changing method according to the second aspect embodiments of the present invention has at least the following beneficial effects:

[0028] When changing the second reagent for coating operation, this liquid changing method can first introduce the cleaning reagent into the coating blade head through the first driver to clean the residual first reagent, and then discharge the first reagent and the cleaning reagent in the coating blade head out of the coating blade head through the emptying channel by the second driver, so as to effectively reduce the residual amount of the first reagent in the coating blade head and reduce the liquid pollution caused by liquid changing.

[0029] According to some embodiments of the present invention, the second reagent is the same as the cleaning reagent.

[0030] According to some embodiments of the present invention, the liquid changing assembly further includes a drying assembly movably disposed relative to the coating blade head; before the second liquid adding step, the liquid changing method further includes:

[0031] Driving the drying assembly to move relative to the coating blade head and jetting gas toward the liquid outlet to dry the liquid outlet.

[0032] According to some embodiments of the present invention, the first liquid adding step and the second liquid adding step further include:

[0033] Real-time monitoring of the bubbles in the first groove, and driving the second driver to empty the first groove when bubbles are detected.

[0034] Additional aspects and advantages of the present invention will be given in part in the following description, become apparent in part from the following description, or be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention will be further described below in conjunction with the drawings and embodiments, where:

[0036] Figure 1 is a three-dimensional structural schematic diagram of a coating blade head according to some embodiments of the present invention;

[0037] Figure 2 is Figure 1 an enlarged structural schematic diagram of part A in

[0038] Figure 3 is Figure 1 an enlarged structural schematic diagram of part B in

[0039] Figure 4 is Figure 1 an exploded structural schematic diagram of the coating blade head of

[0040] Figure 5 is Figure 4 an enlarged structural schematic diagram of part C in

[0041] Figure 6 is Figure 4 an enlarged structural schematic diagram of part D in

[0042] Figure 7 is Figure 4 another perspective schematic diagram of

[0043] Figure 8 is a sectional view schematic diagram of a coating blade head according to some embodiments of the present invention;

[0044] Figure 9 is another sectional view schematic diagram of a coating blade head according to some embodiments of the present invention;

[0045] Figure 10 Another cross-sectional schematic diagram of the coating knife head according to some embodiments of the present invention;

[0046] Figure 11 Schematic diagram of the drying component of the present invention drying the liquid outlet of the coating knife head.

[0047] Reference numerals:

[0048] Coating knife head 1000; first block 100; first groove 110; liquid outlet 111; second groove 120; first liquid separation cavity 121; liquid guide groove 130; liquid inlet channel 140; liquid inlet connector 141; evacuation channel 150; evacuation connector 151; fourth groove 160; recess 170; control valve 180;

[0049] Second block 200; third groove 210;

[0050] Drying component 2000; gas outlet 2100. Detailed implementation manners

[0051] The embodiments of the present invention will be described in detail below. The examples of the embodiments are shown in the drawings, where the same or similar reference numerals represent the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present invention and should not be construed as a limitation of the present invention.

[0052] In the description of the present invention, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present invention.

[0053] In the description of the present invention, the meaning of several is one or more, the meaning of multiple is more than two, greater than, less than, exceeding, etc. are understood as not including the present number, and above, below, within, etc. are understood as including the present number. If the first and second are described only for the purpose of distinguishing technical features, they should not be construed as indicating or implying relative importance or implicitly indicating the quantity of the indicated technical features or the sequence of the indicated technical features.

[0054] In the description of the present invention, unless otherwise clearly defined, words such as setting, installing, connecting, etc. should be understood in a broad sense, and those skilled in the art can reasonably determine the specific meanings of the above words in the present invention in combination with the specific content of the technical solution.

[0055] In the related art, the sample carrier includes a reaction surface, and the corresponding biochemical reaction is completed by wetting the reaction surface with a reagent reaction solution. In order to reduce the amount of the reaction solution used, in an existing liquid distribution method, a coating cutter head is directly used to distribute the reaction solution onto the reaction surface. The coating cutter head includes two blocks. After the two blocks are joined together, the liquid outlet channel of the coating cutter head is located between the two blocks. However, in the above structure, after using the coating cutter head to distribute a reaction solution onto the reaction surface of the sample carrier, there is still some reaction solution remaining in the coating cutter head. When it is necessary to use the coating cutter head to distribute another reaction solution onto the reaction surface of the sample carrier, the previous reaction solution remaining in the coating cutter head will contaminate the current reaction solution, affecting the accuracy of the biochemical reaction result.

[0056] Therefore, the present invention provides a liquid changing assembly and a liquid changing method, which can reduce the risk of liquid contamination caused by liquid changing.

[0057] Referring to Figures 1 to 11 , in an embodiment of the first aspect of the present invention, a liquid changing assembly is provided. The liquid changing assembly includes a coating cutter head 1000, and the coating cutter head 1000 is used to distribute a liquid (the liquid may include a reagent for reacting with the reaction surface and a cleaning solution for cleaning the reagent, etc.) onto the reaction surface of the sample carrier. The coating cutter head 1000 includes a first block 100 and a second block 200. The first block 100 includes a first side wall, and the second block 200 includes a second side wall. The first side wall of the first block 100 is fitted and connected to the second side wall of the second block 200, that is, the first side wall and the second side wall are closely attached. In some embodiments, the first block 100 and the second block 200 can be connected by pins and bolts. The pins and bolts penetrate through the first block 100 and the second block 200, and cause the first block 100 and the second block 200 to be mutually extruded, that is, the first side wall and the second side wall are mutually extruded. The first side wall and the second side wall only need to meet the requirement of mutual fitting, and their specific shapes are not limited. In some embodiments, both the first side wall and the second side wall are planar. In other embodiments, both the first side wall and the second side wall can be wavy, and the shapes between the first side wall and the second side wall are complementary to each other, so as to achieve their close attachment.

[0058] Referring to Figures 4 to 6, the first side wall is provided with a first groove 110. The first groove 110 extends downward to the side of the first side wall. When the first side wall and the second side wall are fitted together, a liquid outlet channel is formed between the first groove 110 and the second block 200, and a liquid outlet 111 is formed between the port of the first groove 110 extending to the side and the second block 200. At least one of the first block 100 and the second block 200 is provided with a liquid inlet channel 140 communicating with the first groove 110. The liquid inlet channel 140 can be connected with a liquid inlet connector 141. External liquid can enter the coating head 1000 through the liquid inlet channel 140 and fill the first groove 110, so that the liquid can be guided from the liquid inlet channel 140 to the liquid outlet channel and finally be discharged from the liquid outlet 111 of the coating head 1000. The liquid sprayed onto the reaction surface of the sample carrier through the liquid outlet 111 is uniform, and the film thickness of the liquid can reach 5 - 10 μm. In some embodiments, the first groove 110 can directly form the liquid outlet channel with the first side wall. In other embodiments, a groove can also be provided at the position of the second side wall opposite to the first groove 110. This groove communicates with the first groove 110 and jointly forms the liquid outlet channel with the first groove 110. The first block 100 or the second block 200 is also provided with a drainage channel 150. The drainage channel 150 communicates with the first groove 110. The liquid or air bubbles in the first groove 110 can be discharged to the outside of the coating head 1000 through the drainage channel 150. Of course, a control valve 180 such as a solenoid valve can be installed in the drainage channel 150. The inlet of the control valve 180 communicates with the drainage channel 150, and the outlet of the control valve 180 can be connected with a drainage connector 151. In the normal state, the drainage channel 150 is in a non-conductive state. When it is necessary to drain the residual liquid in the coating head 1000, the drainage channel 150 can be made conductive, so that the liquid or air bubbles in the first groove 110 can be discharged to the outside of the coating head 1000 through the drainage channel 150.

[0059] Of course, the liquid changing assembly can also include a first driver and a second driver. The first driver is used to drive the external liquid to the liquid inlet channel 140 and make the liquid be discharged from the liquid outlet 111 after passing through the liquid outlet channel. The second driver is used to drive the liquid in the first groove 110 to be discharged from the coating head 1000 through the drainage channel 150. The first driver and the second driver can be liquid pumps or other types of drivers.

[0060] The coating blade head 1000 is provided with a drainage channel 150 in the first block 100 or the second block 200. The drainage channel 150 communicates with the first groove 110. After using the coating blade head 1000 to dispose a liquid on the reaction surface of the sample carrier, when it is necessary to use the coating blade head 1000 to dispose another liquid on the reaction surface of the sample carrier, the residual previous liquid in the coating blade head 1000 can be discharged to the outside through the drainage channel 150, thereby reducing the risk of liquid contamination caused by liquid replacement and being beneficial to improving the accuracy of the biochemical reaction result. Moreover, the structure of the coating blade head 1000 is simple, easy to be mass-produced by machining, and has a low cost.

[0061] Referring to Figure 5 and Figure 6 , it can be understood that in order to make the liquid entering the coating blade head 1000 through the liquid inlet channel 140 be evenly distributed in the first groove 110. In some embodiments of the present invention, the first side wall is further provided with a liquid guiding groove 130. The liquid guiding groove 130 communicates with a side of the first groove 110 away from the liquid outlet 111. The depth of the liquid guiding groove 130 is greater than the depth of the first groove 110. The liquid inlet channel 140 communicates with one end of the liquid guiding groove 130 along the length direction, and the drainage channel 150 communicates with the other end of the liquid guiding groove 130 along the length direction. When the liquid enters the liquid guiding groove 130 through the liquid inlet channel 140, since the depth of the liquid guiding groove 130 is greater than the depth of the first groove 110, the liquid can first fill the liquid guiding groove 130 along the length direction of the liquid guiding groove 130 and then be introduced into the first groove 110. At this time, the liquid can enter the first groove 110 simultaneously in the length direction of the liquid guiding groove 130, so that the distribution of the liquid in the first groove 110 is more uniform, which is beneficial to improving the liquid discharging uniformity of the coating blade head 1000. Moreover, since the liquid inlet channel 140 communicates with one end of the liquid guiding groove 130 along the length direction and the drainage channel 150 communicates with the other end of the liquid guiding groove 130 along the length direction, when the liquid enters the coating blade head 1000 from the liquid inlet channel 140, it can flow from one end of the liquid guiding groove 130 to the other end, so as to quickly fill the entire liquid guiding groove 130. When it is necessary to drain the residual liquid in the coating blade head 1000, the drainage channel 150 is conducted to generate negative pressure, and the residual liquid in the liquid guiding groove 130 and a part of the residual liquid in the first groove 110 can flow to the end of the liquid guiding groove 130 and enter the drainage channel 150, and finally be discharged to the outside of the coating blade head 1000, and the draining efficiency is higher.

[0062] It can be understood that, in order to prevent the liquid in the liquid guide groove 130 from flowing into the evacuation channel 150 and discharging outside the coating head 1000 during the coating operation, in some embodiments of the present invention, the end of the evacuation channel 150 away from the liquid guide groove 130 is inclined upward, so that after the liquid enters one end of the evacuation channel 150 from the liquid guide groove 130, it is difficult for the liquid to continue flowing upward along the evacuation channel 150, thereby preventing the liquid from flowing out of the evacuation channel 150.

[0063] Referring to Figure 4 , it should be noted that the gap between the first block 100 and the second block 200 is very small. Under the capillary action, the liquid may leak upward along the gap. For this reason, in some embodiments of the present invention, the first side wall is provided with a second groove 120. The second groove 120 is provided on the side of the first groove 110 away from the liquid outlet 111 and is spaced from the first groove 110. A first liquid isolation cavity 121 is formed between the second groove 120 and the second block 200. When the liquid penetrates upward, the liquid can enter the first liquid isolation cavity 121, thereby preventing the liquid from continuing to penetrate upward. Of course, it is also possible that the second side wall is provided with a second groove 120. The second groove 120 is provided on the side of the first groove 110 away from the liquid outlet 111 and is spaced from the first groove 110. A first liquid isolation cavity 121 is formed between the second groove 120 and the first block 100.

[0064] It can be understood that, in order to further improve the blocking effect on the upwardly penetrating liquid, in some embodiments of the present invention, the first side wall is provided with a second groove 120. The second groove 120 is provided on the side of the first groove 110 away from the liquid outlet 111 and is spaced from the first groove 110. A first liquid isolation cavity 121 is formed between the second groove 120 and the second block 200. And the second side wall is provided with a third groove 210. The third groove 210 is provided on the side of the first groove 110 away from the liquid outlet 111. A second liquid isolation cavity is formed between the third groove 210 and the first block 100. By providing the first liquid isolation cavity 121 and the second liquid isolation cavity, the coating head 1000 can increase the capacity of the upwardly penetrating liquid, thereby improving the blocking effect on the upwardly penetrating liquid. Moreover, since grooves are provided on both the first side wall and the second side wall, the liquid penetrating upward along the first side wall and the second side wall can be accommodated in the corresponding liquid isolation cavities, thereby greatly improving the blocking effect on the upwardly penetrating liquid.

[0065] It can be understood that, in some embodiments of the present invention, the first liquid isolation cavity 121 and the second liquid isolation cavity are spaced apart, so as to achieve double blocking of the upwardly penetrating liquid and greatly improve the blocking effect. Or the first liquid isolation cavity 121 and the second liquid isolation cavity at least partially overlap. At this time, the first liquid isolation cavity 121 is communicated with the second liquid storage, so as to increase the capacity of the liquid isolation cavity and improve the blocking effect on the liquid.

[0066] It can be understood that, in order to improve the ability of the first liquid separation cavity 121 and / or the second liquid separation cavity to continuously accommodate the permeated liquid, in some embodiments of the present invention, a fourth groove 160 is provided at the end of the coating cutter head 1000 along the length direction, and the fourth groove 160 communicates with the second groove 120 and / or the third groove 210. For example, the fourth groove 160 communicates with the second groove 120, and the liquid in the second groove 120 can flow into the fourth groove 160, so that the second groove 120 can continuously accommodate the liquid permeating upward. Of course, it is also possible that the fourth groove 160 communicates with both the second groove 120 and the third groove 210 at the same time. At this time, the liquid in both the second groove 120 and the third groove 210 can flow into the fourth groove 160. Two fourth grooves 160 can be provided, and the two fourth grooves 160 are respectively located on the side walls at both ends of the coating cutter head 1000 along the length direction, so as to greatly improve the ability of the first liquid separation cavity 121 and / or the second liquid separation cavity to continuously accommodate the permeated liquid.

[0067] Refer to Figure 1 and Figure 2 , it should be noted that, in order to prevent the liquid in the fourth groove 160 from flowing back to the liquid outlet 111 and contaminating the liquid at the liquid outlet 111, in some embodiments of the present invention, a concave portion 170 is provided at the lower end of the coating cutter head 1000. The concave portion 170 is located between the liquid outlet 111 and the fourth groove 160, and the concave portion 170 is recessed upward. Therefore, under the action of gravity, the liquid in the fourth groove 160 cannot flow through the concave portion 170 to the liquid outlet 111, so as to prevent the liquid in the fourth groove 160 from flowing back to the liquid outlet 111 and contaminating the liquid at the liquid outlet 111. Of course, two concave portions 170 can also be provided, and the two concave portions 170 are respectively located outside both ends of the liquid outlet 111, so as to prevent the liquid in the fourth grooves 160 at both ends of the coating cutter head 1000 along the length direction from flowing back to the liquid outlet 111.

[0068] It can be understood that, in order to prevent the liquid from adhering to the outer side wall of the liquid outlet 111, in some embodiments of the present invention, the coating cutter head 1000 has a hydrophobic coating, and the hydrophobic coating is provided on the outer side wall of the liquid outlet 111, and the hydrophobic coating can prevent the liquid from adhering thereto. Of course, in order to improve the uniformity of the coated liquid, the inner wall of the liquid outlet channel can be polished, so that the inner wall of the liquid outlet channel is more hydrophilic, which is beneficial to the flow of the liquid thereon, thereby improving the coating uniformity.

[0069] Refer to Figure 11, It should be noted that, in order to quickly clean the liquid outlet 111 of the coating blade head 1000, in some embodiments of the present invention, the liquid changing assembly further includes a drying assembly 2000 movably arranged relative to the coating blade head 1000. The drying assembly 2000 includes a gas outlet 2100 for jetting gas towards the liquid outlet 111 to dry the liquid outlet 111. After the coating blade head 1000 finishes the coating operation, the drying assembly 2000 can be started, and then gas is jetted towards the liquid outlet through the gas outlet 2100 to blow away or dry the remaining liquid at the liquid outlet, so that the liquid outlet can be quickly dried, which is beneficial to further reducing the liquid pollution caused by liquid changing. Specifically, the drying assembly 2000 can move along the length direction of the liquid outlet, so as to more thoroughly blow dry or blow away the remaining liquid at various parts of the liquid outlet.

[0070] It should be noted that some refrigerated reagent liquids are prone to generate bubbles, or bubbles are generated during the process of injecting liquid into the coating blade head 1000 by the first driver, or for other reasons, resulting in the presence of bubbles in the first groove 110 and affecting the uniformity of liquid coating of the coating blade head 1000. For this reason, in some embodiments of the present invention, the liquid changing assembly further includes a sensor for detecting bubbles. The sensor is arranged in the first groove 110 and is communicatively connected with the second driver. When the sensor detects the presence of bubbles in the first groove 110, the second driver is started, so that the evacuation channel 150 is conducted to evacuate the bubbles in the first groove 110, thereby reducing the influence of bubbles on coating.

[0071] An embodiment of the second aspect of the present invention provides a liquid changing method. This liquid changing method uses the liquid changing assembly of the embodiment of the first aspect of the present invention for liquid changing. The liquid changing method includes the following steps:

[0072] The first liquid adding step: Driving the first driver. At this time, the first driver can introduce the first reagent from the outside into the coating blade head 1000 through the liquid inlet channel, and then the first reagent is distributed to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet;

[0073] The liquid changing and cleaning step: Driving the first driver. At this time, the first driver can introduce the cleaning reagent from the outside into the coating blade head 1000 through the liquid inlet channel, so as to rinse the remaining first reagent in the coating blade head 1000; and driving the second driver, the second driver can discharge the first reagent and the cleaning reagent in the first groove out of the coating blade head 1000 through the evacuation channel, thereby reducing the pollution caused by liquid changing;

[0074] The second liquid adding step: Closing the second driver, and introducing the second reagent into the coating blade head 1000 through the first driver through the liquid inlet channel and distributing the second reagent to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet.

[0075] This liquid changing method can first introduce a cleaning reagent into the coating blade head 1000 through a first driver to clean the residual first reagent when changing the second reagent for coating operation, and then discharge the first reagent and the cleaning reagent in the coating blade head 1000 from the coating blade head 1000 through an emptying channel through a second driver, thereby effectively reducing the residual amount of the first reagent in the coating blade head 1000 and reducing liquid contamination caused by liquid changing.

[0076] It should be noted that in the above embodiments, in order to reduce the impact of the cleaning reagent on the second reagent, in some embodiments of the present invention, the second reagent is the same as the cleaning reagent. Therefore, even if some cleaning reagent remains in the coating head 1000, since the cleaning reagent is the same as the second reagent, the impact on the second reagent in the next coating operation can be reduced, which is beneficial to improving the reliability of the reaction results.

[0077] It should be noted that, since the liquid outlet is far from the emptying channel, after the second driver empties the coating blade 1000, some liquid may remain at the liquid outlet and be difficult to discharge. For this reason, in some embodiments of the present invention, the liquid replacement component also includes a drying component 2000 that is movably arranged relative to the coating blade 1000. Before the second liquid adding step, the liquid replacement method also includes: driving the drying component 2000 to move relative to the coating blade 1000 and spraying gas toward the liquid outlet to dry the liquid outlet, so that the residual liquid at the liquid outlet can be blown dry or blown away, thereby further reducing the pollution caused by liquid replacement. Specifically, the drying component 2000 can move along the length direction of the liquid outlet, so that the residual liquid at various places of the liquid outlet can be blown dry or blown away more thoroughly.

[0078] It should be noted that some refrigerated reagent liquids are prone to bubbles, or bubbles are generated during the process of injecting liquid into the coating blade 1000 through the first driver, or bubbles are generated for other reasons, resulting in bubbles in the first groove 110 and affecting the uniformity of the coating liquid applied by the coating blade 1000. To this end, in some embodiments of the present invention, the first liquid addition step and the second liquid addition step also include: real-time monitoring of the bubbles in the first groove, and driving the second driver to empty the first groove when the bubbles are detected, thereby reducing the influence of the bubbles on the reagent reaction results. Specifically, the liquid replacement component is provided with a sensor for detecting bubbles, the sensor is arranged in the first groove 110, and the sensor is connected to the second driver for communication. When the sensor detects the presence of bubbles in the first groove 110, the second driver is started, so that the emptying channel 150 is turned on and the bubbles and liquid in the first groove 110 are emptied, thereby reducing the influence of bubbles on the coating.

[0079] The coating blade head 1000 has been described in detail in conjunction with the accompanying drawings for the embodiments of the present invention. However, the present invention is not limited to the above embodiments, and various changes can be made without departing from the gist of the present invention within the scope of knowledge possessed by those of ordinary skill in the relevant technical field.

Claims

1. A liquid changing assembly for dispensing liquid to a reaction surface of a sample carrier, characterized in that, The liquid changing assembly includes a coating blade head, and the coating blade head includes: A first block, including a first side wall, where the first side wall is provided with a first groove, and the first groove extends downward to the side of the first side wall; and, A second block, including a second side wall that fits with the first side wall. A liquid outlet channel is formed between the first groove and the second block, and a liquid outlet is formed between the port of the first groove extending to the side and the second block; At least one of the first block and the second block is provided with a liquid inlet channel communicating with the first groove, and the liquid inlet channel is in communication with the liquid; Wherein, the first block or the second block is further provided with a drainage channel communicating with the first groove; The liquid changing assembly further includes a first driver and a second driver. The first driver is used to guide the liquid to the liquid inlet channel and discharge the liquid from the liquid outlet after passing through the liquid outlet channel; the second driver is used to drive the liquid in the first groove to be discharged from the coating blade head through the drainage channel.

2. The liquid changing assembly according to claim 1, wherein The first side wall is further provided with a liquid guiding groove, and the liquid guiding groove communicates with a side of the first groove away from the liquid outlet. The depth of the liquid guiding groove is greater than the depth of the first groove. The liquid inlet channel communicates with one end of the liquid guiding groove along the length direction, and the drainage channel communicates with the other end of the liquid guiding groove along the length direction.

3. The liquid exchange assembly according to claim 2, characterized in that, The drainage channel is inclined upward in a direction away from the liquid guiding groove.

4. The liquid changing assembly according to claim 1, wherein The first side wall is provided with a second groove, which is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove. A first liquid separation cavity is formed between the second groove and the second block; or, the second side wall is provided with a second groove, which is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove. A first liquid separation cavity is formed between the second groove and the first block.

5. The liquid changing assembly according to claim 4, characterized in that, The first side wall is provided with the second groove, which is arranged on a side of the first groove away from the liquid outlet and is spaced from the first groove. A first liquid separation cavity is formed between the second groove and the second block; The second side wall is provided with a third groove, which is arranged on a side of the first groove away from the liquid outlet. A second liquid separation cavity is formed between the third groove and the first block; The first liquid separation cavity and the second liquid separation cavity are arranged at intervals or at least partially overlap.

6. The liquid changing assembly according to claim 5, characterized in that, A fourth groove is provided at the end of the coating blade head along the length direction, and the fourth groove communicates with the second groove and / or the third groove.

7. The liquid changing assembly according to claim 6, characterized in that, A concave portion is provided at the lower end of the coating blade head, and the concave portion is located between the liquid outlet and the fourth groove.

8. The liquid exchange assembly according to claim 1, characterized in that, The coating blade head has a hydrophobic coating, and the hydrophobic coating is provided on the outer side wall of the liquid outlet.

9. The liquid changing assembly according to any one of claims 1 to 8, characterized in that, The liquid changing assembly further includes a drying assembly movably arranged relative to the coating blade head. The drying assembly includes a gas outlet for spraying gas toward the liquid outlet to dry the liquid outlet.

10. The liquid changing assembly according to claim 9, characterized in that, The liquid changing assembly further includes a sensor for detecting air bubbles. The sensor is disposed in the first groove and is communicatively connected to the second driver.

11. A liquid changing method using the liquid changing assembly according to any one of claims 1 to 10, characterized in that, Comprising: First liquid adding step: driving the first driver to introduce a first reagent into the coating cutter head through the liquid inlet channel, and distributing the first reagent to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet. Liquid changing and cleaning step: driving the first driver to introduce a cleaning reagent into the coating cutter head through the liquid inlet channel, and driving the second driver to discharge the first reagent and the cleaning reagent in the first groove through the evacuation channel. And Second liquid adding step: closing the second driver, and introducing a second reagent into the coating cutter head through the first driver through the liquid inlet channel, and distributing the second reagent to the reaction surface of the sample carrier through the liquid outlet channel and the liquid outlet.

12. The liquid exchange method according to claim 11, wherein The second reagent is the same as the cleaning reagent.

13. The liquid replacement method according to claim 11, wherein The liquid changing assembly further includes a drying assembly movably disposed relative to the coating cutter head. Before the second liquid adding step, the liquid changing method further includes: Driving the drying assembly to move relative to the coating cutter head and jetting gas toward the liquid outlet to dry the liquid outlet.

14. The liquid exchange method according to claim 11, characterized in that, The first liquid adding step and the second liquid adding step further include: Real-time monitoring of air bubbles in the first groove, and driving the second driver to evacuate the first groove when air bubbles are detected.

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