Preparation process of nanoscale water-soluble squalene

CN120210296BActive Publication Date: 2026-05-26ANHUI AIWEI BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ANHUI AIWEI BIOTECH CO LTD
Filing Date
2025-03-27
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

In the current technology, squalene is scarce in resources, has high cost and low purity, and is poor in water solubility and easily oxidized, which affects its application in cosmetics, nutritional supplements, functional foods and pharmaceutical products.

Method used

Using corn flour as raw material, fermentation conditions were optimized by combining ionic liquid and compound enzymatic hydrolysis with ultrasonic treatment. Squalene was extracted by supercritical CO2 extraction and molecular distillation technology, and its stability and water solubility were improved by nano-processing. A dynamic covalent cross-linked layer was formed by encapsulation with modified β-cyclodextrin and Schiff base reaction.

Benefits of technology

This enables low-cost, sustainable squalene production, increases yield and purity, enhances its solubility and bioavailability in water, imparts targeting properties, and extends shelf life.

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Abstract

This invention belongs to the field of bioengineering technology, specifically relating to a preparation process for nano-sized water-soluble squalene. The process includes the following steps: S1, corn flour is treated and then mixed with pure water, followed by enzymatic hydrolysis using a compound enzyme to obtain a corn saccharification liquid for later use; S2, *Saccharomyces cerevisiae* GS-A3 is inoculated into a culture medium containing the corn saccharification liquid for fermentation; S3, after centrifugation of the fermentation broth, crude squalene is obtained by supercritical CO2 extraction; S4, after molecular distillation, the product is nano-sized. This invention uses corn as raw material, utilizing abundant starch resources, converting it into glucose through enzymatic hydrolysis, and producing squalene in *Saccharomyces cerevisiae* via the MVA pathway. This invention prepares high-yield, high-purity squalene, and through a special nano-saccharification process, improves the stability of squalene, extends its shelf life during application, and significantly improves its bioavailability. Furthermore, the technology used in this invention meets the requirements of green environmental protection.
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