Performance detection device for ultralow-temperature cell cryopreservation membrane

Abstract

The invention relates to the technical field of cell cryopreservation membrane detection, in particular to an ultralow-temperature cell cryopreservation membrane performance detection device which comprises a fixing seat, the fixing seat is located at the bottom end of the whole device, a top block is installed on the fixing seat, a lifting assembly is installed between the top block and the fixing seat, and a detection table is fixedly installed at the top end of the top block. According to the detection device provided by the device, a simulation freezer is powered off and the environment is heated in the heating process of a light source heating head, after several minutes, the detection device is rotated to the next station, a cell cryopreservation membrane is observed through a microscope lens, a cell membrane slide is placed on a detection disc, and the detection disc is rotated through a rotating assembly at the bottom end; according to the cell membrane slide glass detection device, the cell membrane slide glass can be conveniently and rotationally detected, the detection efficiency is improved, the arranged rotating assembly is located on the detection table, the height of the detection table can be adjusted through the lifting assembly at the bottom end, a user can conveniently adjust the height according to the height of the user, and the detection convenience is facilitated.

Description

Technical Field

[0001] The present invention relates to the technical field of cell cryopreservation membrane detection. Specifically, it relates to a performance detection device for cryogenic cell cryopreservation membranes. Background Art

[0002] Ultra-low temperature cell freezing membranes are generally used to hold and preserve cell fluid, achieving the effect of preserving cell fluid. Cell cryopreservation is a technique that places cells in a low-temperature environment through a cryoprotective mechanism, reduces cell metabolism, and maintains cell viability for long-term storage.

[0003] Currently, after the ultra-low temperature cell freezing membrane is stored, it is necessary to detect and observe its cell fluid. Most of the current detection devices detect one by one through a microscope, and during the detection process, only the front side of the ultra-low temperature cell freezing membrane can be detected. The ultra-low temperature cell freezing membrane cannot be adjusted in angle, and it is also impossible to conveniently observe the dynamic situation of the internal cell fluid. Moreover, when the cell fluid is being preserved, it is also very important to maintain the ultra-low temperature environment temperature. When the low-temperature equipment fails, the environmental temperature will rise rapidly. This requires the freezing membrane to have a certain heat preservation effect, so that the temperature rise rate inside the freezing membrane (bag and box) can be minimized during the equipment repair period to ensure cell viability. Conventional detection devices can only simply observe the cell state and do not have the function of locally simulating environmental temperature rise. Therefore, improvements are needed to meet more comprehensive detection functions. Summary of the Invention

[0004] The purpose of the present invention is to provide a performance detection device for ultra-low temperature cell cryopreservation membranes to solve the problems raised in the above background art.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A performance detection device for cryogenic cell cryopreservation membranes, including a fixed base. The fixed base is located at the bottom of the entire device. A top block is installed on the fixed base. A lifting component is installed between the top block and the fixed base. A detection table is fixedly installed at the top of the top block. A rotating component is installed inside the detection table. A connecting column is installed on the rotating component, and a detection disk is fixedly installed at the top of the connecting column. The detection disk is located at the opening groove in the middle of the detection table. A number of cell membrane carriers for loading cell fluid by the cryopreservation membrane are installed on the detection disk. The top of the cell membrane carrier is detected by a light source heating head and a microscope lens. The light source heating head and the microscope lens are on a fixed frame, and the fixed frame is fixedly installed on the detection table. A plurality of storage grooves are opened on the detection disk. Side grooves are opened in the storage grooves, and a jacking component is installed in the side grooves. A first movable frame and a second movable frame are respectively installed on the lifting assembly. The first movable frame and the second movable frame are rotationally connected by a rotating shaft. A cylinder is fixedly installed on the fixed seat. A pneumatic rod is installed at the output end of the cylinder, and the protruding end of the top of the pneumatic rod is sleeved through a kit on the first movable frame and is rotationally connected through the rotating shaft. A first motor is installed on the rotating assembly. A main shaft is installed at the output end of the first motor, and a first bevel gear is fixedly installed on the main shaft. A second bevel gear is engaged at the bottom end of the first bevel gear, and the second bevel gear is located on the chassis. The jacking assembly is horizontally arranged in the side groove. The jacking assembly includes a second motor. A threaded rod is installed at the output end of the second motor. Two threaded blocks are respectively threadedly connected to the threaded rod, and a first jacking block and a second jacking block are respectively connected to the two threaded blocks. The first jacking block and the second jacking block respectively support and contact the left and right ends of the bottom of the cell membrane slide.

[0006] Further, a slide rail is provided on the chassis of the present invention. A movable member and a sliding block are respectively slidably connected to the slide rail, and a first motor is fixedly installed on the sliding block.

[0007] Further, a connecting frame is provided between the main shaft at the output end of the first motor of the present invention and the movable member, and a connecting column for connecting the detection disk is fixedly installed at the top end of the connecting frame.

[0008] Further, side sliding grooves are respectively opened at the inner sides of the fixed seat and the top block of the present invention. The outer sides of the first movable frame and the second movable frame are fixedly installed with protruding blocks and are slidably connected to the side sliding grooves.

[0009] Further, a panel is fixedly installed on the front of the detection table of the present invention. The first motor and the cylinder are both controlled by the panel.

[0010] Further, the threaded rod of the present invention is located on the bottom block, and a sliding groove is opened on the bottom block. The sliding groove is slidably connected to the protruding block at the bottom end of the threaded block.

[0011] Compared with the prior art, the present invention has the following beneficial effects: (1) The present invention is a performance detection device for cryopreservation membranes of ultra-low temperature cells. The detection device provided by this device cuts off the power supply of the simulation freezer during the temperature rise process of the light source heating head, and the environment warms up. After a few minutes, it turns to the next station and observes the cell cryopreservation membrane through a microscope lens. The cell membrane slide is placed on the detection disk, and the detection disk rotates through the rotating assembly at the bottom end, which is convenient for rotary detection of the cell membrane slide and improves the detection efficiency. (2) The present invention is a performance detection device for cryopreservation membranes of ultra-low temperature cells. The rotating assembly provided in this device is located on the detection table, and the detection table can be adjusted in height through the lifting assembly at the bottom, which is convenient for the user to adjust the height according to their own height to facilitate the convenience of detection.

[0012] (3) The present invention is a performance detection device for cryopreservation membranes of ultra-low temperature cells. The jacking assembly provided in this device can jack up small structures at one angle or multiple angles, which is convenient for observing the internal cell flow state to detect whether the internal cell dynamic performance will be immediately affected after the external light causes temperature rise, so as to detect the performance of this cryopreservation membrane bag itself in turn. Brief Description of the Drawings

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0014] Figure 1 It is a schematic structural diagram of a performance detection device for cryopreservation membranes of ultra-low temperature cells according to an embodiment of the present invention; Figure 2 It is a schematic structural diagram of the rotating assembly of a performance detection device for cryopreservation membranes of ultra-low temperature cells according to an embodiment of the present invention; Figure 3 It is according to the present invention Figure 2 The enlarged structural diagram at A in; Figure 4 It is a schematic structural diagram of the detection disk in a performance detection device for cryopreservation membranes of ultra-low temperature cells of the present invention; Figure 5 It is a schematic structural diagram of the jacking assembly in the present invention.

[0015] Reference Signs: 1, fixed seat; 2, first movable frame; 3, second movable frame; 4, rotating shaft; 5, cylinder; 6, air rod; 7, raised block; 8, side chute; 9, top block; 10, detection table; 11, panel; 12, detection disk; 13, cell membrane slide; 14, fixing frame; 15, light source heating head; 16, chassis; 17, slide rail; 18, movable part; 19, sliding block; 20, first motor; 21, main shaft; 22, first bevel gear; 23, second bevel gear; 24, connecting frame; 25, connecting column; 26, placement groove; 27, microscope lens; 28, side end groove; 29, bottom block; 30, bottom raised block; 31, second motor; 32, threaded rod; 33, threaded block; 34, first jacking block; 35, second jacking block; 36, chute. Detailed Embodiments

[0016] The following is a detailed description of the embodiments of the present invention. The embodiments are implemented on the premise of the technical solution of the present invention, and detailed implementation manners and specific operation processes are given. However, the protection scope of the present invention is not limited to the following embodiments.

[0017] As Figures 1 to 5 shown in the performance detection device of the low-temperature cell cryopreservation membrane, which includes a fixed seat 1. The fixed seat 1 is located at the bottom end of the entire device. A top block 9 is installed on the fixed seat 1. A lifting component is installed between the top block 9 and the fixed seat 1. A detection table 10 is fixedly installed at the top end of the top block 9. A rotating component is installed in the detection table 10. A connecting column 25 is installed on the rotating component, and a detection disk 12 is fixedly installed at the top end of the connecting column 25. The detection disk 12 is located at the slot in the middle of the detection table 10. A number of cell membrane carriers 13 loaded with cell liquid by the cryopreservation membrane are installed on the detection disk 12; The top end of the cell membrane carrier 13 is detected by a light source heating head 15 and a microscope lens 27. The light source heating head 15 and the microscope lens 27 are located on a fixed frame 14, and the fixed frame 14 is fixedly installed on the detection table 10. A plurality of placement slots 26 are opened on the detection disk 12. A side end slot 28 is opened in the placement slot 26, and a jacking component is installed in the side end slot 28; A first movable frame 2 and a second movable frame 3 are respectively installed on the lifting component. The first movable frame 2 and the second movable frame 3 are rotatably connected by a rotating shaft 4. A cylinder 5 is fixedly installed on the fixed seat 1. A pneumatic rod 6 is installed at the output end of the cylinder 5, and the protruding end at the top of the pneumatic rod 6 is sleeved through a kit on the first movable frame 2 and rotatably connected through the rotating shaft 4; A first motor 20 is installed on the rotating component. A main shaft 21 is installed at the output end of the first motor 20, and a first bevel gear 22 is fixedly installed on the main shaft 21. A second bevel gear 23 is engaged at the bottom end of the first bevel gear 22. The second bevel gear 23 is located on a chassis 16; The jacking component is horizontally arranged in the side end slot 28. The jacking component includes a second motor 31. A threaded rod 32 is installed at the output end of the second motor 31. Two threaded blocks 33 are respectively threadedly connected to the threaded rod 32. A first jacking block 34 and a second jacking block 35 are respectively connected to the two threaded blocks 33. The first jacking block 34 and the second jacking block 35 respectively support and contact the left and right ends of the bottom of the cell membrane carrier 13.

[0018] A slide rail 17 is provided on the chassis 16. A movable member 18 and a sliding block 19 are respectively slidably connected to the slide rail 17. The first motor 20 is fixedly installed on the sliding block 19.

[0019] A connecting frame 24 is provided between the output end main shaft 21 of the first motor 20 and the movable member 18, and a connecting column 25 for connecting and detecting the disk 12 is fixedly installed at the top end of the connecting frame 24.

[0020] Side sliding grooves 8 are formed in the inner sides of the fixed seat 1 and the top block 9, and protruding blocks 7 are fixedly installed at the outer sides of the first movable frame 2 and the second movable frame 3 and are slidably connected to the side sliding grooves 8.

[0021] A panel 11 is fixedly installed on the front surface of the detection table 10, and the first motor 20 and the air cylinder 5 are both controlled by the panel 11.

[0022] The threaded rod 32 is located on the bottom block 29, a sliding groove 36 is formed in the bottom block 29, and the sliding groove 36 is slidably connected to the protruding block 30 at the bottom end of the threaded block 33.

[0023] Working principle: 1. The detection device provided in this device observes the cell cryopreservation membrane through the light source heating head 15 and the microscope lens 27. Among them, the light source heating head 15 locally heats the cell membrane slide loaded with cell liquid on the lower station through strong light illumination, simulating the failure of the refrigeration equipment, and reaching the preset heating time. 2. The detection disk 12 rotates through the rotating assembly at the bottom end. The rotating assembly drives the rotation of the first bevel gear 22 on the main shaft 21 through the drive of the first motor 20. Since the second bevel gear 23 is engaged with the bottom end of the first bevel gear 22, and the first motor 20 is slidably connected to the slide rail 17 on the chassis 16 through the sliding block 19. When the first motor 20 drives the main shaft 21 to rotate, it will drive itself to rotate, thereby realizing the rotation of the detection disk 12 on the connecting column 25 at the top end of the connecting frame 24, facilitating the rotary detection of the cell membrane slide 13. At this time, the cell membrane slide 13 that has reached the preset heating time is rotated to the next station, and is just observed through the microscope lens 27 above this station to check the changes of the cells in the cell membrane slide after the simulation of the refrigeration equipment failure and local heating, so as to detect and analyze whether the performance of the cryopreservation membrane loaded with cell liquid meets the standards.

[0024] 3. To observe from multiple angles and the flow state of cell sap, the jacking assembly is horizontally arranged in the side slot 28. The rotation of the threaded rod 32 is driven by the second motor 31. Since the bottom ends of the two threaded blocks 33 threadedly connected to the threaded rod 32 are both slidably connected to the chute 36 through the bottom raised blocks 30, the first jacking block 34 and the second jacking block 35 on the two threaded blocks 33 are simultaneously moved forward in the same direction. When the first jacking block 34 jacks up the cell membrane slide 13 on which the cell cryopreservation membrane is placed, the cell membrane slide 13 can be tilted in one direction. Conversely, the other end of the cell membrane slide 13 can be tilted. The small structure can be jacked up at one angle or multiple angles, which is convenient for observing the internal cell flow state. To detect whether the internal cell dynamic performance will be immediately affected after the external light is heated up, so as to detect the performance of this cryopreservation membrane bag itself; Among them, the first jacking block 34 and the second jacking block 35 can also adjust the preset spacing according to the actual size of the cell membrane slide 13, with good matching degree, and can be applied to the detection of cell membrane slides 13 of various different sizes; The set rotating assembly is on the detection table 10. The detection table 10 can be adjusted in height through the lifting assembly at the bottom. The adjustment of the first movable frame 2 on the air rod 6 is driven by the cylinder 5 to drive the air rod 6. Since the first movable frame 2 and the second movable frame 3 are rotatably connected through the rotating shaft 4, and both sides of the first movable frame 2 and the second movable frame 3 are slidably connected to the side chutes 8 on both sides of the fixed seat 1 and the top block 9 through the raised blocks 7, the height adjustment of the detection table 10 is realized, which is convenient for the user to adjust the height according to his own height for the convenience of detection.

Claims

1. Performance detection device for cryopreservation membrane of ultra-low temperature cells, characterized in that, It includes a fixed base (1), the fixed base (1) is located at the bottom end of the whole device, a top block (9) is installed on the fixed base (1), a lifting component is installed between the top block (9) and the fixed base (1), a detection table (10) is fixedly installed at the top of the top block (9), a rotating component is installed in the detection table (10), a connecting column (25) is installed on the rotating component, and a detection disk (12) is fixedly installed at the top of the connecting column (25). The detection disk (12) is located at the slot in the middle of the detection table (10), and a number of cell membrane slides (13) loaded with cell fluid by a cryopreservation film are installed on the detection disk (12). The top of the cell membrane slide (13) is detected by a light source heating head (15) and a microscope lens (27). The light source heating head (15) and the microscope lens (27) are located on a fixed frame (14), and the fixed frame (14) is fixedly installed on the detection table (10). A plurality of placement slots (26) are formed in the detection disk (12), a side-end slot (28) is formed in the placement slot (26), and a jacking component is installed in the side-end slot (28). A first movable frame (2) and a second movable frame (3) are respectively installed on the lifting component. The first movable frame (2) and the second movable frame (3) are rotatably connected by a rotating shaft (4). A cylinder (5) is fixedly installed on the fixed base (1). The output end of the cylinder (5) is provided with a pneumatic rod (6), and the protruding end at the top of the pneumatic rod (6) is sleeved through a kit on the first movable frame (2) and rotatably connected through the rotating shaft (4). A first motor (20) is installed on the rotating component. The output end of the first motor (20) is provided with a main shaft (21), and a first bevel gear (22) is fixedly installed on the main shaft (21). The bottom of the first bevel gear (22) is engaged with a second bevel gear (23), and the second bevel gear (23) is located on a chassis (16). The jacking component is horizontally arranged in the side-end slot (28). The jacking component includes a second motor (31). The output end of the second motor (31) is provided with a threaded rod (32). Two threaded blocks (33) are respectively threadedly connected to the threaded rod (32). A first jacking block (34) and a second jacking block (35) are respectively connected to the two threaded blocks (33). The first jacking block (34) and the second jacking block (35) respectively support and contact the left and right ends of the bottom of the cell membrane slide (13).

2. The performance detection device for the ultra-low temperature cell cryopreservation membrane according to claim 1, characterized in that, A slide rail (17) is provided on the chassis (16). An active part (18) and a sliding block (19) are respectively slidably connected to the slide rail (17). A first motor (20) is fixedly installed on the sliding block (19).

3. The performance detection device for the ultra-low temperature cell cryopreservation membrane according to claim 1, characterized in that, A connecting frame (24) is provided between the main shaft (21) at the output end of the first motor (20) and the active part (18). The top of the connecting frame (24) is fixedly installed with a connecting column (25) connecting the detection disk (12).

4. The performance detection device of the ultra-low temperature cell cryopreservation membrane according to claim 1, wherein, Both the inner ends of the fixed seat (1) and the top block (9) are provided with side sliding grooves (8), and the outer ends of the first movable frame (2) and the second movable frame (3) are fixedly installed with protruding blocks (7) which are slidably connected with the side sliding grooves (8).

5. The performance detection device for the ultra-low temperature cell cryopreservation membrane according to claim 1, wherein A panel (11) is fixedly installed on the front of the detection table (10), and both the first motor (20) and the cylinder (5) are controlled by the panel (11).

6. The performance detection device for the ultra-low temperature cell cryopreservation membrane according to claim 1, characterized in that, The threaded rod (32) is located on the bottom block (29), and a sliding groove (36) is formed on the bottom block (29), and the sliding groove (36) is slidably connected with a protruding block (30) at the bottom end of the threaded block (33).

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