Device and method for increasing working distance of objective lens of microscope
By designing a microscope device including a movable connection of objective lens fixing bracket and lens holder, the working distance of the objective lens is extended by using convex lenses or concave lenses, the problem of insufficient working distance of existing microscopes at medium and high magnitudes is solved, and normal observation and focus of conventional culture plates and glass slides is achieved, which reduces experimental costs and ensures working accuracy.
Patent Information
- Application Number
- CN202510364743.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-26
- Publication Date
- 2025-06-27
AI Technical Summary
Under medium and high-power objective lenses, the working distance of the objective lens is smaller than the thickness of conventional culture plates and slides, resulting in the inability to focus observation, and may even damage the sample, affecting the experimental results.
A device including a movably connected objective lens fixing bracket and lens holder is designed to extend the working distance of the microscope objective to accommodate culture plates and slides of different thicknesses by selecting convex or concave lenses and adjusting their position.
It realizes normal observation and focus of conventional culture plates and slides under medium and high-power objectives, reducing experimental costs and ensuring working accuracy.
Smart Images
Figure CN120215103A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of microscope equipment, and relates to a device for increasing the working distance of a microscope objective lens. The present invention also relates to a method for increasing the working distance of a microscope objective lens. Background Art
[0002] An optical microscope is an important tool for people to understand the microscopic world, and is also an essential scientific instrument for implementing optical precision measurement, analyzing microscopic tissues, observing fine structures, and carrying out micro processes. A traditional optical microscope includes an imaging system for magnifying a sample and an illumination system for irradiating the sample. With the progress of science and technology, optical microscopes have also experienced a growth process from simple to complex, such as fluorescence microscopes, confocal microscopes, super-resolution microscopes, etc., bringing more possibilities for studying the microscopic world.
[0003] In biological research, biological samples are usually carried on culture dishes (plates), conventional glass slides, etc. However, for currently sold microscope equipment, such as microscopes of Olympus, Zeiss, etc., when the objective lens is greater than 20 times, the distance from the top of the objective lens to the sample surface (working distance) is often less than 0.17 mm, and the working distance is less than the thickness of a conventional culture dish (plate) and a glass slide (0.5 - 1 mm), resulting in inability to focus and observe, contact between the eyepiece and the culture plate, and even damage to the sample beyond the limit, which has an obvious adverse impact on the experimental results. Although the application of special culture well plates such as confocal dishes can solve these problems within a limited range, their cost is high, which is more than ten times that of ordinary culture plates, restricting their application. Therefore, to solve such problems, it is urgent to design a new structure to increase the working distance of the objective lens of the microscope, improve the working efficiency of the microscope under medium and high magnification lenses, and save experimental costs. Summary of the Invention
[0004] The object of the present invention is to provide a device for increasing the working distance of a microscope objective lens, which solves the problem in the prior art that when the working distance of a medium and high magnification objective lens of a microscope is less than the thickness of a conventional culture plate and a glass slide, it is easy to touch the culture plate or the glass slide.
[0005] Another object of the present invention is to provide a method for increasing the working distance of a microscope objective lens, which solves the problem in the prior art that when the working distance of a medium and high magnification objective lens of a microscope is less than the thickness of a conventional culture plate and a glass slide, it is easy to touch the culture plate or the glass slide, resulting in a large error in the experimental results.
[0006] The technical solution adopted by the present invention is that a device for increasing the working distance of a microscope objective lens includes an objective lens fixing bracket and a lens holder that are movably connected. The lens holder is in the shape of a disc with an axial hole, and a lens is placed in the lens holder; the objective lens fixing bracket is in the shape of a ring, and a plurality of support legs are arranged on the outer circumference of the objective lens fixing bracket.
[0007] Another technical solution adopted by the present invention is a method for increasing the working distance of a microscope objective lens. The device for increasing the working distance of the microscope objective lens is used and implemented according to the following steps: Step 1: Select a convex lens or a concave lens according to the situation; Step 2: Place the lens selected in Step 1 into the card slot of the lens holder; Step 3: Mate the external thread of the lens holder with the internal thread of the objective lens fixing bracket so that the connection between the lens holder and the objective lens fixing bracket is reliable; Step 4: Fix the assembly on the upper part of the objective lens through the support legs and clamping blocks on the objective lens fixing bracket; finally, rotate the lens holder to change the relative height between the lens and the objective lens fixing bracket, and find the appropriate focal plane, then it is completed.
[0008] The beneficial effect of the present invention is that when the working distance of medium and high magnification objective lenses is less than the thickness of conventional culture plates and glass slides, the working distance of the microscope objective lens is increased, enabling normal use of non-special culture dishes (plates) under the objective lens to observe and focus on samples, saving experimental costs and ensuring working accuracy. Brief Description of the Drawings
[0009] Figure 1 is the overall structure diagram of the device for increasing the working distance of the microscope objective lens of the present invention; Figure 2 is the exploded structure diagram of the device for increasing the working distance of the microscope objective lens of the present invention; Figure 3 is the application structure principle diagram of the present invention's device using a convex lens; Figure 4 is the distance increase analysis diagram of the present invention's device using a concave lens; Figure 5 is the object distance - image distance curve diagram of the present invention's device using a convex lens; Figure 6 is the object distance - image distance curve diagram of the present invention's device using a concave lens.
[0010] In the figures, 1. assembly; 2. lens; 3. lens holder; 4. groove; 5. card slot; 6. objective lens fixing bracket; 7. internal thread; 8. support leg; 9. clamping block; 10. objective lens; 11. external thread. Detailed Description of the Invention
[0011] The present invention will be described in detail below in conjunction with the drawings and specific embodiments.
[0012] Refer to Figure 1 、 Figure 2, the structure of the device of the present invention includes an objective lens fixing bracket 6 and a lens holder 3 which are movably connected. The lens holder 3 is in the shape of a disc with an axial hole. Along the upper edge of the axial hole of the lens holder 3, there is a low-step card slot 5 (equivalent to a groove), and a lens 2 is placed in the card slot 5; on the upper surface of the high step of the lens holder 3, there are stripe-shaped grooves 4 arranged along the circumferential direction; on the outer circular surface of the lens holder 3, there is an external thread 11; The objective lens fixing bracket 6 is in the shape of a ring. On the inner circular surface of the objective lens fixing bracket 6, there is an internal thread 7, and the internal thread 7 is correspondingly sleeved with the external thread 11 on the outer circular surface of the lens holder 3; on the outer circular surface of the objective lens fixing bracket 6, a plurality of support legs 8 (3 - 4 support legs 8 can be set according to needs) are evenly fixed along the circumferential direction, and on the lower inner surface of each support leg 8, there is a clamping block 9.
[0013] The lens 2 is selected as a convex lens or a concave lens as the case may be, and different refractive index lenses are replaced according to the magnification of the objective lens 10. The conditions for selecting a convex lens or a concave lens for the lens 2 (extended glass slide) are: when the distance from the orifice plate to the lens 2 of this device is more than 15 mm, a convex lens is used. According to Figure 5 the curve, when the object distance of the convex lens is more than 15 mm, it is the effective working range, and it is relatively easy to adjust the ratio of the object distance to the image distance; when the distance from the orifice plate to the lens 2 of this device is within 15 mm, a concave lens is used. According to Figure 6 the curve, the object distance of the concave lens is the effective range starting from 0, so a concave lens is used.
[0014] The lens holder 3 is used to support and load the lens 2. There is a card slot 5 for placing the lens 2 in the lens holder 3. During use, the lens 2 is directly placed on it, and the lens 2 directly contacts and rests in the card slot 5 by gravity; on the lens holder 3, there are stripe-shaped grooves 4, which are used to increase the friction force to facilitate the installation of the lens holder 3 on the inner circular surface of the objective lens fixing bracket 6.
[0015] On the inner circular surface of the objective lens fixing bracket 6, there is an internal thread 7, which is used to connect the lens holder 3. It is connected with the external thread 11 of the lens holder 3 by a threaded socket connection method, and can adjust the relative height of the lens holder 3 and the objective lens fixing bracket 6, so as to facilitate the adjustment of the focal length of the assembly 1; On the outer circumference of the objective lens fixing bracket 6, there are a plurality of support legs 8; the clamping block 9 on the inner side of the lower part of the support leg 8 is an inverted hook-shaped elastic component. All the support legs 8 directly clamp on the objective lens 10 through their respective clamping blocks 9 to realize the fixation and positioning of the assembly 1.
[0016] The working principle of the device of the present invention is divided into two methods according to whether the lens 2 is a convex lens or a concave lens, that is, the working principle of the convex lens and the working principle of the concave lens, which are described as follows: First, the working principle of the convex lens: Refer toFigure 3 , the lens 2 of the convex lens is adopted in the combination 1 of the present invention. Among them, a is the simulated optical path diagram, b is the focal plane after using the combination 1, c is the position where the lens 2 is placed, d is the focal plane without using the combination 1, e is the vertex plane position of the objective lens 10 of the microscope, and f is the focal position of the objective lens 10 after using the combination 1 of the present invention, which has increased from the original d to the position of a, that is, the position where the sample morphology can be clearly seen. It can be seen that when using the lens 2 of the convex lens, e is the vertex plane position of the objective lens 10 of the microscope. Normally, the working focal plane position of the objective lens 10 is d. When the lens 2 of the combination 1 is fixed to the position of c, the focal plane position of the objective lens 10 moves up from d to b, and the optical path diagram is as Figure 3 shown in a in the figure, thus achieving the effect of increasing the working distance of the objective lens 10 of the microscope.
[0017] The principle of the optical path diagram using the convex lens is as follows: The focal point d is the focal plane of the objective lens 10. The design idea mainly focuses on the position of d to extend the light rays that originally converge at the focal point d. When the sample is placed at the position f (plane b), the light rays pass through the lens 2 at the position c. After refraction by the lens 2, the light rays converge again at the plane d. The original focal point at d becomes the focal point at b. Thus, the effect of increasing the working distance of the objective lens 10 of the microscope is achieved.
[0018] The main formula for using the convex lens is the Gaussian imaging theorem (1 / f = 1 / u + 1 / v), where f is the focal length, u is the object distance, and v is the image distance. When a convex lens with a fixed focal length f = 10 mm is selected, 1 / 10 = 1 / u + 1 / v. When u is the x-axis and v is the y-axis, the image relationship between the object distance and the image distance is as shown in Figure 5.
[0019] Second, the working principle of the concave lens: Refer to Figure 4 , the lens 2 of the concave lens is adopted in the combination 1 of the present invention. Among them, a is the simulated optical path diagram, c is the focal plane after using the combination 1, d is the position of the lens 2 in the combination 1, e is the vertex plane position of the objective lens 10 of the microscope, and f is the focal position of the objective lens 10 after using the combination 1, which has increased from the original d to the position of c, that is, the position where the sample morphology can be clearly seen. It can be seen that when using the lens 2 of the concave lens, e is the vertex plane position of the objective lens 10 of the microscope. Normally, the focal plane position of the objective lens 10 is d, that is, at the top of the position where the lens 2 of the combination 1 is placed. When the lens 2 of the combination 1 is fixed to the position of d, at this time, the focal plane position of the objective lens 10 moves up to c, and the optical path diagram is as Figure 4 shown in a in the figure, thus achieving the effect of increasing the working distance of the objective lens 10 of the microscope.
[0020] The optical path diagram principle using a concave lens is as follows: The position of the focal plane of the objective lens 10 is at the top of the current device placement position (i.e., at the intersection of the reverse extension lines of the refracted light rays). The main design idea focuses on extending the converging light rays at the focal point. When the sample is placed at position f (plane c), the light rays pass through lens 2 at position d and are refracted by lens 2, changing the optical path of the light rays to the optical path that should originally be placed on the focal plane of the objective lens 10. The focal plane originally at the top of d is moved up to the position of focal plane c, thereby achieving the effect of increasing the working distance of the objective lens 10 of the microscope.
[0021] The main formula for using a concave lens is the Gaussian imaging theorem (1 / f = 1 / u + 1 / v), where f is the focal length, u is the object distance, and v is the image distance. When a convex lens with a fixed focal length f = -10 mm is selected, -1 / 10 = 1 / u + 1 / v. When u is on the x-axis and v is on the y-axis, the image relationship between the object distance and the image distance is as shown in Figure 4, where the positive value of the x-axis is taken as the positive object distance.
[0022] The method for increasing the working distance of the microscope objective in the present invention is implemented using the aforementioned device according to the following steps: Step 1: Select a convex lens or a concave lens according to the situation.
[0023] The principle for selecting a convex lens or a concave lens for lens 2 is as follows: When the distance from the orifice plate to lens 2 of the present device is more than 15 mm, a convex lens is used. (This 15 mm refers to the distance from the orifice plate to lens 2 of the present device.) According to Figure 5 the curve, when the object distance of the convex lens is more than 15 mm, it is the effective working range, and it is relatively easy to adjust the ratio of the object distance to the image distance. When the distance from the orifice plate to lens 2 of the present device is within 15 mm, a concave lens is used. According to Figure 6 the curve, the object distance of the concave lens is the effective range starting from 0, so a concave lens is used.
[0024] Step 2: Place the lens 2 selected in Step 1 into the card slot 5 of the lens holder 3, and the two are directly supported and connected by gravity; Step 3: Mate the external thread 11 of the lens holder 3 with the internal thread 7 of the objective fixing bracket 6, and by means of the frictional force generated by the striped groove 4 on the upper surface of the lens holder 3, screw the lens holder 3 onto the inner circular surface of the objective fixing bracket 6; Step 4: Fix the assembly 1 on the upper part of the objective lens 10 through the support legs 8 and their clamping blocks 9 on the objective lens fixing bracket 6. The fixing method mainly realizes the clamping and fixing of the objective lens fixing bracket 6 and the objective lens 10 through the rubber barb-like structure of the clamping block 9. Finally, rotate the lens holder 3 to change the lens 2 and the relative height between the objective lens fixing bracket 6, which is equivalent to changing the relative position between the assembly 1 and the objective lens 10, and find a suitable focal plane again to implement the next operation of the microscope.
[0025] Example 1 I. Experimental preparation: Refer to Figure 3 , in the assembly 1 of this Example 1, the lens 2 uses a convex lens. The experimental conditions are a conventional laboratory environment, and the experimental object is an ordinary culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 10x objective lens, and then clamp the assembly 1 of the present invention on the objective lens 10 of the microscope as required.
[0026] II. Method for selecting the device lens: In this Example 1, uniformly use lenses with a focal length of 10 mm for the time being, so there is no need to select lenses.
[0027] III. Final distance of the culture well plate: According to the determined f = 10 mm, v is greater than or equal to 2 times the object distance = 20 mm. According to the formula 1 / 10 = 1 / u + 1 / 20, it is obtained that u = 20 mm. Therefore, after using the assembly 1 of the present invention, the working distance of the microscope can be extended to 20 mm to achieve the purpose of use.
[0028] IV. Adopt the device and steps described above of the present invention to complete the relevant microscope focal length adjustment operations.
[0029] Example 2 I. Experimental preparation: Refer to Figure 3 , in the assembly 1 of this Example 1, the lens 2 uses a convex lens. The experimental conditions are a conventional laboratory environment, and the experimental object is an ordinary culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 20x objective lens, and then clamp the assembly 1 of the present invention on the objective lens 10 of the microscope as required.
[0030] II. Method for selecting the device lens: In this Example 2, uniformly use lenses with a focal length of 10 mm for the time being, so there is no need to select lenses.
[0031] III. Final distance of the culture well plate: According to the determined f = 10 mm, v is slightly greater than 2 times the object distance = 22.5 mm. According to the formula 1 / 10 = 1 / u + 1 / 22.5, it is obtained that u = 18 mm. Therefore, after using the assembly 1 of the present invention, the working distance of the microscope can be extended to 18 mm to achieve the purpose of use.
[0032] IV. Use the device and steps described above in the present invention to complete the relevant microscope focal length adjustment operations.
[0033] Example 3 I. Experiment preparation: Refer to Figure 3 , the lens 2 in the combination body 1 of the present invention uses a convex lens. The experimental conditions are a conventional laboratory environment, and the experimental object is a common culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 40x objective lens, and then clamp this device on the objective lens 10 of the microscope as required.
[0034] II. Method for selecting the device lens: In this Example 3, uniformly use lenses with a focal length of 10 mm for the time being, so there is no need to select lenses.
[0035] III. Final distance of the culture well plate: According to the determined f = 10 mm, v is greater than 2 times the object distance = 25 mm. According to the formula 1 / 10 = 1 / u + 1 / 25, u = 16.67 mm is obtained. Therefore, after using the combination body 1 of the present invention, the working distance of the microscope can be extended to 16.67 mm, achieving the usage purpose.
[0036] IV. Use the device and steps described above in the present invention to complete the relevant microscope focal length adjustment operations.
[0037] Example 4 I. Experiment preparation: Refer to Figure 3 , the lens 2 in the combination body 1 of the present invention uses a concave lens. The experimental conditions are a conventional laboratory environment, and the experimental object is a common culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 10x objective lens, and then clamp this device on the objective lens 10 of the microscope as required.
[0038] II. Method for selecting the device lens: In this Example 4, uniformly use lenses with a focal length of -10 mm for the time being, so there is no need to select lenses.
[0039] III. Final distance of the culture well plate: According to the determined f = -10 mm, the virtual image v is closer to 1 times the focal length = -6 mm. According to the formula 1 / -10 = 1 / u + 1 / -6, u = 15 mm is obtained. Therefore, after using the combination body 1 of the present invention, the working distance of the microscope can be extended to 15 mm, achieving the usage purpose.
[0040] IV. Use the device and steps described above in the present invention to complete the relevant microscope focal length adjustment operations.
[0041] Example 5 I. Experiment preparation: Refer to Figure 4, the lens 2 in the assembly 1 of the present invention is a concave lens. The experimental conditions are a conventional laboratory environment, and the experimental object is an ordinary culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 20x objective lens, and then clamp the assembly 1 of the present invention on the objective lens 10 of the microscope as required.
[0042] II. Method for selecting the lens of the device: In this Embodiment 5, lenses with a unified focal length of -10 mm are temporarily used, so there is no need to select lenses.
[0043] III. Final distance of the culture well plate: According to the determined f = -10 mm, the virtual image v is closer to the middle between 1 focal length and 0 point = -5 mm. According to the formula 1 / -10 = 1 / u + 1 / -5, u = 10 mm is obtained. Therefore, after using the assembly 1 of the present invention, the working distance of the microscope can be extended to 10 mm, achieving the purpose of use.
[0044] IV. Using the device and steps described above in the present invention, complete the relevant microscope focal length adjustment operations.
[0045] Embodiment 6 I. Experimental preparation: Refer to Figure 3 , the lens 2 in the assembly 1 of the present invention is a concave lens. The experimental conditions are a conventional laboratory environment, and the experimental object is an ordinary culture well plate containing biological samples. First, adjust the objective lens 10 of the microscope to a 40x objective lens, and then clamp the assembly 1 of the present invention on the objective lens 10 of the microscope as required.
[0046] II. Method for selecting the lens of the device: In this Embodiment 6, lenses with a unified focal length of -10 mm are temporarily used, so there is no need to select lenses.
[0047] III. Final distance of the culture well plate: According to the determined f = -10 mm, the virtual image v is closer to the 0 point position = -4 mm. According to the formula 1 / -10 = 1 / u + 1 / -4, u = 6.67 mm is obtained. Therefore, after using the assembly 1 of the present invention, the working distance of the microscope can be extended to 6.67 mm, achieving the purpose of use.
[0048] IV. Using the device and steps described above in the present invention, complete the relevant microscope focal length adjustment operations.
Claims
1. A device for increasing the working distance of a microscope objective lens, characterized in that: It comprises an objective lens fixing bracket (6) and a lens holder (3) which are movably connected. The lens seat (3) is in the shape of a circular disc with an axial hole, and the lens (2) is placed in the lens seat (3); The objective lens fixing bracket (6) is in the shape of a circular ring, and a plurality of bracket legs (8) are arranged on the outer circumference of the objective lens fixing bracket (6).
2. The device for increasing the working distance of a microscope objective lens according to claim 1, characterized in that: The lens seat (3) is provided with a slot (5) for placing the lens (2).
3. The device for increasing the working distance of a microscope objective lens according to claim 1, characterized in that: The lens seat (3) is provided with stripe-shaped grooves (4).
4. The device for increasing the working distance of a microscope objective lens according to claim 1, characterized in that: The outer circumferential surface of the lens holder (3) is provided with an external thread (11), and correspondingly, the inner circumferential surface of the objective lens fixing bracket (6) is provided with an internal thread (7), and the internal thread (7) is sleeve-connected with the external thread (11) of the lens holder (3).
5. The device for increasing the working distance of a microscope objective lens according to claim 1, characterized in that: A clamping block (9) is installed on the inner side of the lower part of the support leg (8).
6. The device for increasing the working distance of a microscope objective lens according to claim 5, characterized in that: The clamping block (9) is a barbed-hook shaped elastic component.
7. A method for increasing the working distance of a microscope objective lens, using the device for increasing the working distance of a microscope objective lens according to any one of claims 1 to 6, characterized in that: Follow these steps to implement: Step 1: Choose a convex lens or a concave lens according to the situation; Step 2, placing the lens (2) selected in step 1 into the slot (5) of the lens holder (3); Step 3, matching and connecting the external thread (11) of the lens holder (3) with the internal thread (7) of the objective lens fixing bracket (6), so that the lens holder (3) and the objective lens fixing bracket (6) are reliably connected; Step 4: Fix the assembly (1) to the upper part of the objective lens (10) by means of the support legs (8) and the clamping blocks (9) on the objective lens fixing support (6); finally, rotate the lens holder (3) to change the relative height between the lens (2) and the objective lens fixing support (6) to find a suitable focal plane.
8. The method for increasing the working distance of a microscope objective lens according to claim 7, characterized in that: In step 1, the principle of selecting a convex lens or a concave lens for the lens (2) is: when the distance between the aperture plate and the lens (2) is greater than 15 mm, a convex lens is used; when the distance between the aperture plate and the lens (2) is less than 15 mm, a concave lens is used.