Method for preparing pleurotus citrinopileatus mushroom stick material from feed mulberry leaf and lacroton seed mixed micro-storage material and traditional Chinese medicine composite material for milk buffalo

Abstract

The invention discloses a method for preparing a pleurotus citrinopileatus mushroom stick material from a feed mulberry and lacroton seed mixed micro-storage material. The method comprises the following steps: (1) pretreating the feed mulberry and lacroton seed mixed micro-storage material; (2) preparing a pleurotus citrinopileatus culture medium based on a feed mulberry and lacroton bean mixed micro-storage material; (3) activating the strain; (4) biotransformation of pleurotus citrinopileatus; and (5) preparing the pleurotus citrinopileatus mushroom stick material. Mycelium protein nutrients, high-quality straw and pure traditional Chinese medicines are compounded and scientifically matched for the first time, under the synergistic effect, protein absorption and conversion of the milk buffalo can be improved, the lactation performance index of the milk buffalo can be effectively improved, and the feed has positive significance for improving feeding and milk quality of the milk buffalo in the southern region.

Description

Technical Field

[0001] The present invention belongs to the technical field of the use of forage mulberry and lablab bean, and particularly relates to a method for preparing elm oyster mushroom spawn material from a mixed micro-stored material of forage mulberry and lablab bean and a compound traditional Chinese medicine feed for milk buffaloes. Background Art

[0002] As a new type of feed variety resource, forage mulberry has high nutritional value and the function of both feeding and medicine, and has attracted more and more attention. After analysis, its crude protein accounts for 16.34 - 18.75% of the dry matter content, the crude fiber is 33.88%, and it has various vitamins, amino acids and rich flavonoid glycosides, phenols, and organic acids. Among them, the amino acids are complete in variety, reaching 18 kinds, accounting for more than 10% of the dry matter of mulberry leaves; it can be cut 4 times a year, and the fresh plant yield reaches 5 - 8 tons per mu, which is one of the rare multi-functional feed resources with great development potential. At present, there are still some problems in the application of forage mulberry in animal feed. The processing and use methods are single, and the effective components wrapped by lignocellulose in forage mulberry cannot be released, reducing the utilization rate of forage mulberry. Most of them are simply freshly crushed or ensiled and mixed into other feeds for feeding. They have a high crude fiber content, a high lignin content, a low digestibility, and certain anti-nutritional factors, and the feeding effect is not significant, and the nutritional value and medicinal value of forage mulberry are not fully exerted. Especially when forage mulberry is ensiled or micro-stored alone, the water content is too high, the protein is too high, and it is easy to turn black and deteriorate, affecting its palatability and feed intake. Especially, its nature and flavor are bitter, sweet, and cold. When used in large amounts, it must be rationally combined with other raw materials and optimized processing technology to exert its due effect.

[0003] Lablab bean belongs to the leguminous forage in tropical regions. It has a high crude protein content. The whole plant protein content is 17% - 21%, among which the leaf crude protein content is 21% - 36%, and the seed crude protein content is 20% - 28%. The young plants are good green feeds and are liked by animals. Previous studies have found that when lablab bean is micro-stored alone, it will produce peculiar smells, be prone to rot, and have poor micro-storage quality due to low sugar content, resulting in a decrease in feed intake and utilization rate of ruminants such as cattle and sheep. Therefore, it must be reasonably mixed and fermented with other raw materials to reduce moisture and reduce peculiar smells, improve its palatability and digestibility, and improve its use value.

[0004] Edible mushroom sticks contain a large amount of mycelial protein, fat, amino acids, minerals, and mycelial secondary metabolites, and have high nutritional value. In the preparation of traditional concentrate feeds for dairy buffaloes, most use soybean meal as the main protein source, and there are the following main disadvantages: The price of soybean meal is relatively high, and extensive use in the breeding of dairy buffaloes will increase the breeding cost and may bring certain economic pressure. Soybean meal contains some anti-nutritional factors, such as trypsin inhibitors, urease, etc. These anti-nutritional factors will affect the digestion and absorption of nutrients such as protein by dairy buffaloes, and the digestibility in dairy buffaloes is only about 70%. Excessive amounts will have an adverse impact on the growth and health of dairy buffaloes. Although soybean meal has a high protein content, the contents of other nutrients such as energy, minerals, and vitamins are relatively low, and the nutrition is unbalanced. If only soybean meal is used as the main protein source without paying attention to reasonable mixing with other feeds, it is easy to cause unbalanced nutrition in dairy cows, affecting their growth, development, and production performance. The fat content in soybean meal is relatively high. If the storage conditions are improper during storage, it is easy to undergo oxidative rancidity and mildew, producing harmful substances. Cows eating spoiled soybean meal may develop digestive system diseases, affecting health and production performance.

[0005] Therefore, it is feasible to use other plant proteins to replace traditional soybean meal as feed for dairy buffaloes, which can improve their nutritional balance and ensure the health of dairy buffaloes.

[0006] There are few reports on the mixed micro-storage feed of forage mulberry and lablab bean. Shen Shijie [1] (2022) et al. mixed forage mulberry with corn flour for silage; Chen Rongqiang [2] (2024) et al. studied the effects of different compound additives on the silage quality and microbial diversity of the mixture of forage mulberry and king grass; Wu Lijuan [3] (2024) et al. studied the effects of 4 additives on the silage quality and microbial diversity of the mixed storage of forage mulberry and navel orange pomace. There are few reports on feeding dairy buffaloes with the mixed micro-storage feed of forage mulberry. Most are related to beef cattle, dairy cows, and fattening sheep. Mei Ning'an [4] (2014) et al. fed the silage mixture of mulberry branches and leaves to fatten Tan-Han crossbred sheep; Fan Qiwen [5] (2023) et al. studied the effects of replacing the basal diet with different proportions of fermented forage mulberry on the growth performance, apparent nutrient digestibility, blood physiological and biochemical indexes, and serum antioxidant indexes of beef cattle; Li Siqi [6] (2024) et al. studied the effects of forage mulberry on the nutrient digestibility and growth performance of growing and finishing pigs; Guo Jianjun [7] (2011) et al. adding an appropriate amount of mulberry leaves to the feed can increase the milk yield and milk quality of dairy cows; Huang Junpeng [8](2023) and others studied the effects of adding mulberry to feed on the production performance, immune and antioxidant functions, and rumen microbial flora of periparturient dairy cows. Most of the research on Laba beans at home and abroad is related to intercropping, and there are few studies on the application of Laba beans as feed for dairy buffaloes. Wang Bin [9] (2021) et al. studied the effects of different sowing rates of Labrador beans and silage corn on grassland production performance and forage quality; Dong Zhixiao

[10] (2021) et al. studied the effects of intercropping of sweet sorghum with Labrador beans on the yield and quality of mixed forage in Chengdu Plain; Li Wenbin

[11] (2022) et al. studied the effects of different densities of intercropping of Labrador beans and dryland corn on the quality of mixed silage; Gao Yingping

[12] (2023) et al. studied the effects of different film covering forms on feed yield and silage quality of dryland corn-laba bean intercropping; Ke Qiang

[13] (2024) and others studied the effect of mixed sowing ratio on the fermentation quality and aerobic stability of mixed silage of laba beans / silage corn; In summary and review of relevant literature, it was found that there are very few studies on the mixed micro-silage feed of mulberry and laba beans, processing methods and applications, especially in the application of dairy buffaloes.

[0007] Liu Duocai

[14] (2011) Research on the application of fungus bran feed in dairy cattle breeding; Zhang Di

[15] (2017) Effects of seafood mushroom residue silage on cattle metabolism and fecal microbiota; Zheng Dan

[16] (2017) Effects of fresh Juncao and Ganoderma lucidum mycelium fermentation on immune function and antioxidant capacity of dairy cows; Wei Tao

[17] (2018) Nutritional value of Pleurotus eryngii bran and its application in beef cattle fattening; Wang Yu

[18] (2020) Evaluation of the nutritional value of Flammulina velutipes bran and its effect on the production performance of beef cattle; Liu Qiyan

[19] (2020) Effects of fermented Flammulina velutipes bran on the production performance and blood physiological indicators of lactating dairy cows; It is reported that adding an appropriate amount of bran to the feed can increase the dry matter intake, digestibility and milk production of dairy cows, and has a good effect on the milk quality of dairy cows. In summary, there is no research report on the utilization of mixed micro-storage of mulberry and laba beans through biotransformation of Pleurotus citrinopileatus mycelium and combined with Chinese herbal medicine as an unconventional high-quality feed resource for dairy buffaloes. The technology of the present invention has significant progress and is original and advanced.

[0008] References

[0009] [1] Shen Shijie, Zheng Lin, Xu Zeping, et al. Experimental report on mixed silage of mulberry branches and leaves and corn flour[J].

[0010] [2] Chen Rongqiang, Lei Xiaowen, Wu Lijuan, et al. Effects of different compound additives on the quality and microbial diversity of mixed silage of mulberry and king grass forage[J].

[0011] [3] Wu Lijuan, Ou Xiang, Lian Hai, et al. Effects of Four Additives on the Quality and Microbial Diversity of Mixed Silage of Forage Mulberry and Navel Orange Residue [J].

[0012] [4] Mei Ning'an, Liu Zixin, Zhao Hui. Experiment on the Fattening Effect of Hybrid Lambs Fed with Mixed Silage of Mulberry Leaves and Branches [J].

[0013] [5] Fan Qiwen, Guo Wanzheng, Zhao Na, et al. Effects of Replacing Basal Diet with Fermented Forage Mulberry at Different Proportions on Growth Performance, Apparent Digestibility of Nutrients, Blood Physiological and Biochemical Indexes, and Serum Antioxidant Indexes of Beef Cattle [J].

[0014] [6] Li Siqi, Wu Chunyan, Cheng Jiaqi, et al. Effects of Forage Mulberry on Nutrient Digestibility and Growth Performance of Growing-Finishing Pigs [J].

[0015] [7] Guo Jianjun, Li Xiaobin, Qi Xuemei, et al. Effects of Adding Mulberry Leaves to Feed on Milk Yield and Milk Quality of Dairy Cows [J]. [8] Huang Junpeng, Zhao Weiguo, Yang Zhaojun, et al. Effects of Adding Forage Mulberry on Production Performance, Immunity and Antioxidant Function, and Rumen Microbial Flora of Periparturient Dairy Cows [J].

[0016] [9] Wang Bin, Dong Xiu, Li Manyou, et al. Effects of Intercropping Lablab purpureus with Different Seeding Rates and Silage Maize on Grassland Production Performance and Forage Quality [J]. Acta Agrestia Sinica, 2021, 29(04): 828-834.

[0017]

[10] Dong Zhixiao, He Runhao, Kuang Jianyang, et al. Effects of Intercropping Sweet Sorghum and Lablab purpureus in the Chengdu Plain on the Yield and Quality of Mixed Forage [J]. Acta Agrestia Sinica, 2021, 29(07): 1578-1583.

[0018]

[11] Li Wenbin, Chai Mingjie, Zhang Bin, et al. Effects of Intercropping Lablab purpureus with Different Densities and Dryland Maize on the Quality of Mixed Silage [J]. Chinese Feed

[0019] Feed, 2022, (14): 13-18. DOI: 10.15906 / j.cnki.cn11-2975 / s.20221404.

[0020]

[12] Gao Yingping, Zheng Binghui, Liang Tianzhu, et al. Effects of Different Film-Mulching Forms on the Forage Yield and Silage Quality of Intercropping Dryland Maize and Lablab purpureus [J]. Chinese Feed

[0021] Feed, 2023, (04): 78-83. DOI: 10.15906 / j.cnki.cn11-2975 / s.20230419.

[0022]

[13] Ke Qiang, Wang Bin, Wang Tengfei, et al. Effect of mixed sowing ratio on fermentation quality and aerobic stability of mixed silage of Labrador bean / silage corn[J]. Grassland and Grass

[0023] Ping, 2024, 44(01): 131-137. DOI: 10.13817 / j.cnki.cyycp.2024.01.015.

[0024]

[14] Liu Duocai. Research on the application of fungus bran feed in dairy cattle breeding[J].

[0025]

[15] Zhang Di. Effects of seafood mushroom residue silage on cattle metabolism and fecal flora[D].

[0026]

[16] Zheng Dan, Shi Jing, Lin Zhanhui. Effects of fresh Juncao and Ganoderma lucidum mycelium fermentation on immune function and antioxidant capacity of dairy cows[J].

[0027]

[17] Wei Tao. Nutritional value of Pleurotus eryngii bran and its application in beef cattle fattening[D].

[0028]

[18] Wang Yu, Li Han, Gao Jing, et al. Evaluation of nutritional value of Flammulina velutipes bran and its effect on production performance of beef cattle [J].

[19] Liu Qiyan, Wang Yu, Zhang Yifeng, et al. Effects of fermented Flammulina velutipes bran on production performance and blood physiological indicators of lactating dairy cows [J]. Summary of the invention

[0029] The purpose of the present invention is to overcome the problems existing in the prior art and provide a method for preparing citrus pleurotus eryngii mushroom sticks by mixing mulberry and laba beans as feed. The present invention also provides a dairy buffalo composite material containing citrus pleurotus eryngii mushroom sticks and Chinese herbal medicines, thereby improving the utilization rate of mulberry and laba beans as feed.

[0030] In order to achieve the above technical objectives, the method for preparing citrus citrus fungus stick material by mixing mulberry and labrador bean feed micro-storage of the present invention comprises the following steps:

[0031] (1) Pretreatment of mulberry and Labrador bean mixed micro-stock:

[0032] The mulberry and Laba beans are crushed separately by a crusher, and the crushed particles have a diameter of ≤2.5 cm; the mulberry and Laba beans are uniformly mixed according to a certain weight ratio, corn husks are sprayed as a moisture regulator and fermentation substrate, the moisture content of the mixed micro-storage is adjusted to 50%-60%, glucose, saccharifying enzyme, yeast and fermentation bacteria are added to the mixed micro-storage in a certain weight ratio, and the mixed micro-storage is uniformly stirred and mixed, the silage is bagged and sealed, and fermented at room temperature of 25°C-30°C to obtain a pretreated material;

[0033] (2) Preparation of Pleurotus citrinopileatus culture medium based on mixed micro-stock of mulberry and Labrador beans:

[0034] Weigh the pre-treated materials, dried jasmine residues, sugarcane bagasse, barley husks, corn flour, calcium carbonate, magnesium sulfate, sugar, and gypsum powder in a certain proportion; weigh according to the formula ratio. The sugar water and magnesium sulfate need to be dissolved in water first, and then the materials are evenly mixed. The moisture content of the culture medium is 60%-65%; the prepared culture medium needs to be piled up and sealed for 2-4 hours to allow the culture medium to absorb enough water evenly to prevent uneven dryness and wetness of the materials; when filling the bags, the materials are filled while gently compacting to make the surrounding walls of the bags smooth and without gaps to prevent mushrooms from growing from the periphery of the bags; when the materials are filled to a certain height, the surface of the materials is flattened and sealed by the folding method; the culture medium is sterilized immediately after filling; for atmospheric pressure sterilization, when the material bags are loaded into the sterilization furnace, the material surface faces up, and the bags are stacked with bag pressure, leaving a slight gap between the bags; after the material temperature reaches a certain temperature, keep it warm for 10-12 hours and then stop firing;

[0035] (3) Activate the strain: Inoculate Pleurotus citrinopileatus on the slant in a sterile environment and place it in a liquid culture medium for shake flask culture;

[0036] (4) Biological conversion of Pleurotus citrinopileatus: Put the liquid strain in the liquid culture medium into the Pleurotus citrinopileatus culture medium, fill the bags and culture. After inoculation, place it in an incubator at a certain temperature for spawn running. After the mycelium fills the bags, obtain the Pleurotus citrinopileatus spawn-running rods. Specifically, the liquid strain and the Pleurotus citrinopileatus culture medium are inoculated according to a certain mass and cultured at a suitable temperature. After the mycelium grows and fills the bags for several days, obtain the Pleurotus citrinopileatus rods;

[0037] (5) Preparation of Pleurotus citrinopileatus rod material: Apply drying technology to the cultivated Pleurotus citrinopileatus rods for drying treatment. First, conduct hot air drying, and then conduct hot blast stove treatment to keep the original nutrients unchanged, that is, obtain the Pleurotus citrinopileatus rod material.

[0038] Preferably, the weight ratio of the forage mulberry and Lablab purpureus in step (1) is 2:1.

[0039] Preferably, the weight ratio of the glucose, glucoamylase, yeast, and fermentation bacteria in step (1) is 4:1:0.5:0.5.

[0040] Preferably, in step (1), the silage is filled into bags, sealed, and placed at room temperature of 25°C - 30°C for fermentation for 20 days to obtain the pre-treated materials.

[0041] Preferably, in step (2), weigh 70% of the pre-treated materials in step (1), 5% of the dried jasmine residues, 9% of the sugarcane bagasse, 10% of the barley husks, 4% of the corn flour, 0.2% of the calcium carbonate, 0.3% of the magnesium sulfate, 1% of the sugar, and 0.5% of the gypsum powder.

[0042] Preferably, the conditions for hot air drying in step (5) are: the temperature is 100°C, spread out in a single layer, and process for 20 minutes.

[0043] Preferably, the conditions for hot blast stove treatment in step (5) are: dry at 200°C - 250°C for 30 minutes.

[0044] The present invention also provides an application of the Pleurotus citrinopileatus mushroom stick material prepared by the above method in the preparation of a traditional Chinese medicine composite feed for dairy buffaloes. The traditional Chinese medicine composite feed for dairy buffaloes comprises the following raw materials in parts by weight: 850 - 950 parts of Pleurotus citrinopileatus mushroom stick material, 150 - 250 parts of dried papaya, 200 - 300 parts of dried straw of water spinach, 100 - 200 parts of yam residue, 80 - 90 parts of Astragalus membranaceus, 80 - 90 parts of Glycyrrhiza uralensis, 40 - 60 parts of Tetrapanax papyriferus, 20 - 40 parts of Taraxacum mongolicum, 60 - 80 parts of Lonicera japonica, and 40 - 60 parts of Forsythia suspensa.

[0045] Preferably, the traditional Chinese medicine composite feed for dairy buffaloes comprises the following raw materials in parts by weight: 950 parts of Pleurotus citrinopileatus mushroom stick material, 250 parts of dried papaya, 200 parts of yam residue, 300 parts of dried straw of water spinach, 90 parts of Astragalus membranaceus, 90 parts of Glycyrrhiza uralensis, 60 parts of Tetrapanax papyriferus, 40 parts of Taraxacum mongolicum, 80 parts of Lonicera japonica, and 60 parts of Forsythia suspensa.

[0046] Preferably, the traditional Chinese medicines in the traditional Chinese medicine composite feed for dairy buffaloes are all in dry form, and after being pulverized to 80 meshes, they are uniformly stirred and mixed with the Pleurotus citrinopileatus mushroom stick material.

[0047] The above application of the traditional Chinese medicine composite feed for dairy buffaloes has relatively high protein and amino acids, can enhance the digestion and absorption of dairy buffaloes, enhance the lactation performance of dairy buffaloes, has a remarkable effect of promoting lactation, reduces the incidence of mastitis, improves the immunity of the animal body, is applied to the feeding of dairy buffaloes to improve the milk production performance of dairy buffaloes and improve the milk quality.

[0048] Furthermore, the dosage of the traditional Chinese medicine composite feed for dairy buffaloes is 30% of the addition amount in each ton of dairy buffalo concentrate supplement.

[0049] Based on the theory of yin-yang balance in traditional Chinese veterinary medicine in China, the present invention emphasizes the importance of protecting and strengthening the balance of the animal body. Referring to the theory in the Compendium of Traditional Chinese Veterinary Medicine, with the main purposes of nutritional balance, replenishing blood and invigorating qi, improving immunity, antibacterial and anti-inflammatory, dispersing carbuncles and promoting lactation, and promoting the postpartum repair of dairy buffaloes and the rapid recovery of milk, natural, digestible and absorbable, and nutritionally diversified mycelium is selected as the main carrier, and pure traditional Chinese medicines are formulated according to the theory of traditional Chinese veterinary medicine. After strict screening and separate processing, they are combined with modern advanced technological manufacturing methods.

[0050] The Pleurotus citrinopileatus mushroom stick material has relatively high nutritional value, can provide balanced nutrition; has low acid detergent fiber and neutral detergent fiber, which is beneficial to high digestion and absorption rate; has a relatively high calcium ion ratio, which helps to maintain the integrity and stability of cell membranes. As a second messenger, calcium ions activate a series of protein kinases by binding to calmodulin and the like, and then regulate physiological processes such as cell metabolism, secretion, proliferation and differentiation, and promote the milk secretion of dairy buffaloes, etc.; has relatively high crude protein, which can improve the lactation performance of dairy buffaloes.

[0051] Water spinach straw contains certain nutritional components such as crude protein, crude fat, dietary fiber, and a small amount of minerals, which can promote rumen fermentation and acidity regulation in milk buffaloes.

[0052] In traditional Chinese medicine pharmacology, components such as oleanolic acid and ursolic acid in papaya have obvious anti-inflammatory effects. There are various nutritional components and bioactive substances, which help to enhance the immune function of milk buffaloes and improve the body's ability to resist diseases. Papaya has a certain inhibitory effect on a variety of bacteria and viruses. For example, it has antibacterial activity against Escherichia coli, Staphylococcus aureus, etc., and also has a certain inhibitory effect on milk buffalo enteroviruses, etc., which helps to prevent and reduce related infectious diseases. Papain, lipase, etc. contained in papaya can promote the decomposition of proteins and fats, which is helpful for the digestion and absorption of milk buffaloes, and can improve indigestion, loss of appetite, etc.

[0053] Substances such as amylase and polyphenol oxidase in Chinese yam are helpful for the digestive and absorption functions of the spleen and stomach. It contains rich nutritional components, which have a certain tonic effect on kidney yin deficiency, kidney yang deficiency, etc. It contains saponins and mucilage, which have lubricating and moistening effects, so it can benefit the lung qi and nourish the lung yin. Chinese yam contains a large amount of mucin, vitamins, and trace elements, which can promote the lactation performance of milk buffaloes.

[0054] In the Complete Works of Modern Traditional Chinese Veterinary Medicine, astragalus can improve the body's immune function and increase the content of immunoglobulin A; licorice has the effects of diuresis, anti-inflammatory, antipyretic and detoxifying; caulis akebiae mainly contains components such as akebia glycoside, fat, and protein, and components such as akebia glycoside play an important role in exerting the effects of clearing heat and promoting diuresis, promoting lactation, etc., and can effectively promote milk secretion in milk buffaloes; dandelion is cold in nature and enters the liver and stomach meridians, and has the effects of dissipating swelling and nodules, dredging stagnation and promoting lactation. It is an important traditional Chinese medicine for treating breast abscess, and can be used to treat breast abscess, breast carbuncle, red and swollen hard lumps, etc., and also has an improvement effect on the situation of milk blockage and breast distending pain after parturition in milk buffaloes; honeysuckle has an inhibitory effect on a variety of pathogenic bacteria such as Staphylococcus aureus, Streptococcus hemolyticus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae, and Salmonella typhi, can promote the release of adrenal cortical hormones, and has an inhibitory effect on inflammation, which can protect the body's disease resistance, anti-inflammatory ability, postpartum repair and health of milk buffaloes; forsythia can clear heat and reduce swelling, disperse qi stagnation and blood stasis, and is commonly used to treat breast abscess, thyroid tumors and other diseases, and can help disperse lumps and relieve pain. It has an inhibitory effect on a variety of pathogenic microorganisms such as Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, influenza virus, and respiratory syncytial virus, and is also an important guarantee for ensuring the postpartum health and repair of milk buffaloes.

[0055] The present invention combines balanced nutrition with traditional Chinese herbs for tonifying qi and blood, antibacterial and anti-inflammatory, and detumescence and promoting lactation, which is beneficial to the postpartum nutritional supply and the efficacy of traditional Chinese medicine health care in milk buffaloes, and effectively promotes the postpartum recovery of milk buffaloes and improves milk production performance.

[0056] Compared with the prior art, the present invention has the following advantages:

[0057] (1) For the first time, the present invention uses mycelial protein nutrients, high-quality straw and pure traditional Chinese medicine for compound scientific compatibility. Under the synergistic effect, it has the dual effects of nutrition and traditional Chinese medicine, and is both edible and medicinal. It can not only improve the protein absorption and conversion of dairy buffaloes, but also effectively improve the lactation performance indexes of dairy buffaloes, which has positive significance for improving the feeding of dairy buffaloes and milk quality in the southern region.

[0058] (2) The formula of the traditional Chinese medicine compound feed for dairy buffaloes of the present invention is reasonable, and the nutritional components are balanced and comprehensive. They are all plant proteins that are easy to digest and absorb, and can achieve energy-nitrogen balance. The mushroom sticks are rich in protein, amino acids, vitamins and minerals, which can provide sufficient nutrition for dairy buffaloes, improve milk yield and milk quality. It contains a variety of traditional Chinese medicine ingredients and bioactive substances, which have the effects of antibacterial, anti-inflammatory and promoting lactation, improve resistance and reduce the occurrence of diseases. Using the formula feed combined with mushroom sticks and traditional Chinese medicine can reduce the dependence on traditional soybean meal, reduce the feeding cost of feed, efficiently utilize the unconventional high-protein feeds mulberry and lablab bean, and improve their utilization rate. Research shows that the mycelium of Pleurotus citrinopileatus is rich in oligosaccharides and flavonoid glycosides, and does not contain any antibiotics, which can promote the growth of beneficial bacteria in the intestine, thereby improving the utilization rate of nitrogen in the feed, increasing important milk quality indexes such as immunoglobulin and lysozyme in milk, and decreasing the somatic cell count in milk, and ultimately improving the lactation performance of dairy buffaloes. The feed of the present invention can effectively improve the feed utilization rate of dairy buffaloes. The milk yield of dairy buffaloes fed with this feed increases by 8.4%-12.6%, the milk fat rate increases by 2.2%-2.5%, and the milk protein rate increases by 10.3%-14.5%. BRIEF DESCRIPTION OF THE DRAWINGS

[0059] Figure 1 It is a mycelium transformation diagram;

[0060] Figure 2 It is a comparison diagram of inoculated Pleurotus citrinopileatus mycelium and non-inoculated control;

[0061] Figure 3 It is a lablab bean diagram;

[0062] Figure 4 It is a mulberry diagram;

[0063] Figure 5 It is a diagram of the re-compounded culture medium after fermentation;

[0064] Figure 6 It is a diagram of the nutritional component detection report of the mushroom stick material without inoculating Pleurotus citrinopileatus strains (control group) and the mushroom stick material inoculated with Pleurotus citrinopileatus strains (experimental group). DETAILED DESCRIPTION OF THE INVENTION

[0065] Example 1

[0066] SeeFigures 1 - 5 Taking the medium without inoculated Pleurotus citrinopileatus as the control group and the inoculated Pleurotus citrinopileatus mushroom stick material as the experimental group, weigh 2000 g of each group of samples and dry them in a drying oven at 65 °C for 48 hours until constant weight to determine the dry matter (DM) content; crush the dried samples through a sieve (40-mesh sieve) and store them in a self-sealing bag for the determination of nutrient components. The crude protein content is determined by the Kjeldahl method (performed according to the standard of GB / T 6432-2018); the acid detergent fiber and neutral detergent fiber in the samples are determined by NY / T 1459-2007 "Determination of Acid Detergent Fiber in Feed" and GB / T 20806-2006 "Determination of Neutral Detergent Fiber (NDF) in Feed"; the crude fat is determined according to GB / T 6433-2006 "Determination of Crude Fat in Feed"; the crude fiber is determined according to GB / T 6434-2006 "Determination of Crude Fiber Content in Feed - Filtration Method"; the calcium in the samples is determined according to GB / T 6436-2018 "Determination of Calcium in Feed"; each index is repeated 3 times, and the results are expressed as the average value.

[0067] The Pleurotus citrinopileatus mushroom stick material is obtained by the following method:

[0068] (1) Pretreatment of the mixed micro-stored material of Morus alba L. var. multicaulis and Lablab purpureus:

[0069] Use a forage secondary continuous crusher (ZL202121819425.7) to separately crush and process Morus alba L. var. multicaulis and Lablab purpureus, and the crushed particle diameter ≤ 2.5 cm; accurately weigh and evenly mix according to the weight ratio of Morus alba L. var. multicaulis and Lablab purpureus of 2:1, and use spouted corn hulls as the moisture regulator and fermentation substrate to adjust the moisture content of the mixed micro-stored material to 50%. Add 4% glucose, 1% glucoamylase, 0.5% yeast, and 0.5% fermentation bacteria (fermented probiotics of Guangxi Xinmu Biotechnology Co., Ltd.) according to the weight of the mixed micro-stored material, and stir and mix evenly. Pack the silage in bags and seal them, and ferment at room temperature of about 25 °C - 30 °C for 20 days. When opening the bag, the pretreated material should not be moldy or deteriorated and have a good wine fragrance to be ready for use.

[0070] (2) Preparation of the Pleurotus citrinopileatus culture medium based on the mixed micro-stored material of Morus alba L. var. multicaulis and Lablab purpureus:

[0071] Accurately weigh (1) 70% of the material, 5% of dried jasmine residues, 9% of bagasse, 10% of barley husks, 4% of corn flour, 0.2% of calcium carbonate, 0.3% of magnesium sulfate, 1% of sugar, and 0.5% of gypsum powder; accurately weigh according to the formula ratio. Sugar water and magnesium sulfate need to be dissolved in water first, and the materials are evenly mixed. The water content of the culture medium is preferably 60%, that is, when the culture medium is tightly held by hand, water droplets drip from between the fingers. The prepared culture medium needs to be piled up and sealed for 4 hours to allow the culture medium to evenly absorb enough water to prevent uneven dryness and wetness of the materials. When filling the bags, fill the materials while gently compacting to make the surrounding walls of the bags smooth and without gaps to prevent mushrooms from growing from the periphery of the bags. Fill the bags to a height of 17 cm, flatten the surface of the materials, and seal the bags using the folding method. Immediately sterilize after the culture medium is filled. Use atmospheric sterilization. When loading the material bags into the sterilization furnace, the surface of the materials faces upward, stack the bags with bag pressure, and leave a slight gap between the bags. After the material temperature reaches 100 °C, keep warm for 10 hours and then stop the fire.

[0072] (3) Activate the strains: Inoculate Pleurotus citrinopileatus on the slant in a sterile environment and place it in a liquid medium for shake flask culture.

[0073] (4) Biological conversion of Pleurotus citrinopileatus: Put the liquid strains in the liquid medium into the Pleurotus citrinopileatus culture medium, fill the bags for culture, and place them in an incubator at about 25 °C for spawn running after inoculation. Wait until the bags are full to obtain the Pleurotus citrinopileatus spawn-running rods. Specifically, the mass ratio of the liquid strains to the Pleurotus citrinopileatus culture medium is 2:8, and the Pleurotus citrinopileatus spawn-running rods are obtained 30 days after the bags are full.

[0074] (5) The main components of the Pleurotus citrinopileatus culture medium mainly composed of forage mulberry and Lablab purpureus are crude protein, cellulose, and rich trace elements, as well as flavonoids, polyphenols, and polysaccharides. Through the mixing inoculation with a mass ratio of 2:8 of Pleurotus citrinopileatus liquid strains to the culture medium, the mycelium can cover the culture medium in 5 days. The mycelium initially directly uses polysaccharides for growth. A high inoculation amount can inhibit the growth of miscellaneous bacteria. After the mycelium uses up the polysaccharides, it can secrete cellulase to degrade cellulose into the nutrients it needs. Start measuring the crude protein content in the cultivation from the 10th day when the mycelium starts to multiply in large numbers until 30 days before fruiting.

[0075] (6) Preparation of Pleurotus citrinopileatus spawn-running rod materials: Apply the high-efficiency heat source drying technology to dry the cultivated Pleurotus citrinopileatus spawn-running rods. First, perform hot air drying (100 °C, single-layer paving, treatment for 20 minutes). The time is short, which can effectively control the pollution of miscellaneous bacteria. Then, perform high-efficiency and rapid drying (30 minutes) at 200 °C - 250 °C in a hot blast stove to keep the original nutrients unchanged, and thus obtain the Pleurotus citrinopileatus spawn-running rod materials.

[0076] The nutritional components are shown in Figure 6The detection report shows that the nutrient composition data is presented in Table 1. As can be seen from Table 1, after inoculating Pleurotus citrinopileatus Singer strains and culturing them into mycelia, the crude protein increased by 39.47%, and the crude fiber decreased by 27.05%, significantly improving the nutritional quality, increasing the crude protein, and reducing the crude fiber content. This fully demonstrates that through mycelial transformation, the nutritional value of the raw materials can be significantly improved, especially in terms of crude protein.

[0077] Table 1 Comparison Table of Nutrient Compositions

[0078]

[0079]

[0080] Example 2

[0081] The traditional Chinese medicine composite is composed of the following raw materials by weight: 850 parts of Pleurotus citrinopileatus Singer mushroom stick material, 200 parts of dried water spinach straw, 100 parts of Chinese yam residue, 80 parts of Astragalus membranaceus, 80 parts of Glycyrrhiza uralensis Fisch., and 20 parts of Taraxacum mongolicum Hand.-Mazz.

[0082] The conventional concentrate formula for dairy buffalo is: 53% corn, 25% soybean meal, 13% wheat bran, 5% premix, 2.0% calcium hydrogen phosphate, 1.0% sodium bicarbonate, and 1.0% salt.

[0083] The concentrate formula for dairy buffalo diet used in this example is: 30% traditional Chinese medicine composite and 70% conventional concentrate formula for dairy buffalo.

[0084] The Pleurotus citrinopileatus Singer mushroom stick material is obtained by the same method as in Example 1.

[0085] Example 3

[0086] It is basically the same as Example 2, with the differences: 900 parts of Pleurotus citrinopileatus Singer mushroom stick material, 200 parts of dried papaya, and 50 parts of Tetrapanax papyriferus (Hook.) K. Koch.

[0087] Example 4

[0088] It is basically the same as Example 2, with the differences: 900 parts of Pleurotus citrinopileatus Singer mushroom stick material, 200 parts of dried papaya, 200 parts of Chinese yam residue, 50 parts of Tetrapanax papyriferus (Hook.) K. Koch, and 60 parts of Lonicera japonica Thunb.

[0089] Example 5

[0090] It is basically the same as Example 2, with the differences: 950 parts of Pleurotus citrinopileatus Singer mushroom stick material, 250 parts of dried papaya, 200 parts of Chinese yam residue, 300 parts of dried water spinach straw, 90 parts of Astragalus membranaceus, 90 parts of Glycyrrhiza uralensis Fisch., 60 parts of Tetrapanax papyriferus (Hook.) K. Koch, 40 parts of Taraxacum mongolicum Hand.-Mazz., 80 parts of Lonicera japonica Thunb., and 60 parts of Forsythia suspensa (Thunb.) Vahl.

[0091] Control Example 1

[0092] Conventional concentrate formula for dairy buffalo: 53% corn, 25% soybean meal, 13% wheat bran, 5% premix, 2.0% calcium hydrogen phosphate, 1.0% baking soda, 1.0% salt.

[0093] The concentrate formula for dairy buffalo diet used in this example is: 5% Chinese herbal medicine compound and 95% conventional concentrate formula for dairy buffalo.

[0094] Comparative Example 2

[0095] It is basically the same as Example 2, except that: the concentrate formula for dairy buffalo diet used in this example is: 10% Chinese herbal medicine compound and 90% conventional concentrate formula for dairy buffalo.

[0096] Comparative Example 3

[0097] It is basically the same as Example 3, except that: the concentrate formula for dairy buffalo diet used in this example is: 10% Chinese herbal medicine compound and 90% conventional concentrate formula for dairy buffalo.

[0098] Comparative Example 4

[0099] It is basically the same as Example 4, except that: the concentrate formula for dairy buffalo diet used in this example is: 10% Chinese herbal medicine compound and 90% conventional concentrate formula for dairy buffalo.

[0100] Comparative Example 5

[0101] It is basically the same as Example 5, except that: the concentrate formula for dairy buffalo diet used in this example is: 10% Chinese herbal medicine compound and 90% conventional concentrate formula for dairy buffalo.

[0102] As can be seen from Table 2:

[0103] (1) The crude protein in Examples 2 - 5 is significantly higher than that in Comparative Examples 1 and 2, indicating that the feed quality of the present invention is far superior to conventional feeds. Adding Chinese herbal medicine to the feed can increase the content of bioactive natural products in the mycelium culture medium, thereby improving the pharmacological activity of the mycelium. The optimal combination is 950 parts of Pleurotus citrinopileatus mushroom stick material, 250 parts of dried papaya, 200 parts of yam residue, 300 parts of dried hollow vegetable straw, 90 parts of Astragalus membranaceus, 90 parts of Glycyrrhiza uralensis, 60 parts of Tetrapanax papyriferus, 40 parts of Taraxacum mongolicum, 80 parts of Lonicera japonica, and 60 parts of Forsythia suspensa.

[0104] (2) The crude protein in Examples 2 - 5 is also significantly higher than that in Comparative Examples 2 - 5, indicating that an addition amount of 30% is superior to an addition amount of 10%.

[0105] In the above examples and comparative examples, the nutritional components of the concentrate for the diet obtained are shown in Table 2.

[0106] Table 2 Comparison table of nutritional components of concentrate for diet %

[0107]

[0108] Experimental Example:

[0109] Feeding of lactating buffaloes: According to the principle of similar age, parity, lactation days, and milk yield, 270 lactating buffaloes with an average body weight of 480 ± 10 kg were randomly divided into 9 groups, with 30 buffaloes in each group. Feeding started 10 days before parturition, and they were fed in individual stalls, managed uniformly, fed twice a day to ensure there was feed in the trough for free intake, and provided with drinking water. The collection time of all samples started 15 days after parturition, and the lactation period lasted for 150 days. Milking was performed twice a day at 7:00 am and 5:00 pm, and the milk yield was recorded each time after milking. The concentrate supplements in the diet of each group corresponded to Examples 2 - 5 and Comparative Examples 1 - 5, with the same supply amount of 5 kg per head per day. The roughage was corn stover silage and dry straw, with the same supply amount for each head, including 25 kg of corn stover silage and 3 kg of dry straw.

[0110] Collection of buffalo milk samples: Before collecting the milk samples of each cow, wipe the udder and teats clean with a warm wet towel, manually squeeze out the first 60 ml of milk from each teat and discard it. Check whether the milk in each udder quarter is normal, and do not collect samples from udder quarters with abnormal appearance. Then collect 200 ml of milk samples from each udder quarter using a 200 ml saline bottle and store them at 4°C for the determination of other components. Buffalo milk samples were taken once every 2 weeks.

[0111] 1. Detection of milk yield

[0112] Method used: During the feeding period, milking was performed twice a day at 7:00 am and 5:00 pm, and the individual daily milk yield was recorded using an electronic flow meter (accuracy ±0.1 L) each time after milking.

[0113] Samples: Record the daily milk yield of each buffalo in Examples 2 - 5 and Comparative Examples 1 - 5. Each example and comparative example in each group was measured three times, and the average value was taken. Then calculate the average daily milk yield of each buffalo during the entire lactation period, which is the required milk yield. The specific data is shown in Table 3.

[0114] 2. Detection of milk fat percentage

[0115] Method used: Take 200 ml of freshly collected buffalo milk and measure the milk fat percentage using a FOSS MilkoScan FT1 multi-functional dairy product analyzer.

[0116] Samples: Take the refrigerated buffalo milk in Examples 2 - 5 and Comparative Examples 1 - 5 for measurement once every 2 weeks. Each example and comparative example in each group was measured three times, and the average value was taken. Then calculate the milk fat percentage of the buffalo milk produced by the buffaloes during the entire lactation period, which is the required milk fat percentage. The specific data is shown in Table 3.

[0117] 3. Detection of milk protein percentage

[0118] Method adopted: Take 200 ml of freshly collected buffalo milk, and measure the milk protein rate with a FOSS MilkoScan FT1 multi-functional dairy product analyzer;

[0119] Samples: Take the refrigerated buffalo milk in Examples 2-5 and Comparative Examples 1-5 every two weeks for measurement. Each group of examples and comparative examples is measured three times, and the average value is taken. Then calculate the milk protein rate of the buffalo milk produced by the lactating buffaloes throughout the lactation period, which is the required milk protein rate; specific data are shown in Table 3.

[0120] 4. Lactose detection

[0121] Method adopted: Take 200 ml of freshly collected buffalo milk, and measure the lactose with a FOSS MilkoScan FT1 multi-functional dairy product analyzer;

[0122] Samples: Take the refrigerated buffalo milk in Examples 2-5 and Comparative Examples 1-5 every two weeks for measurement. Each group of examples and comparative examples is measured three times, and the average value is taken. Then calculate the lactose of the buffalo milk produced by the lactating buffaloes throughout the lactation period, which is the required lactose; specific data are shown in Table 3.

[0123] Samples: Lactating buffaloes in Examples 1-4 and Comparative Examples 1-6; specific data are shown in Table 1.

[0124] Table 3 is an analysis table of each performance index in each example and each comparative example.

[0125] Table 3 Effects of the Chinese medicine composite in Pleurotus citrinopileatus mushroom sticks on milk yield and milk composition

[0126] Item Milk yield (kg / d) Milk fat (%) Milk protein (%) Lactose (mg / L) Example 2 8.16 8.05 4.42 4.78 Example 3 8.53 8.14 4.65 4.85 Example 4 9.26 8.21 4.94 5.05 Example 5 9.55 8.56 5.03 5.21 Comparative Example 1 7.02 6.24 3.78 3.78 Comparative Example 2 7.12 6.45 4.01 4.16 Comparative Example 3 7.16 7.21 4.35 4.35 Comparative Example 4 7.33 7.42 4.63 4.41 Comparative Example 5 8.03 7.61 4.33 4.53

[0127] It can be seen from Table 3 that compared with Comparative Example 1, the milk yield, milk fat rate, milk protein rate, and lactose in Example 2 have all increased significantly. This is because after reasonably matching the mycelium Chinese medicine compound, it meets the nutritional requirements and production characteristics of the lactating period of buffaloes. The crude protein in the feed has increased by 9.82%. The buffaloes have absorbed sufficient nutrition, making their nutrition more balanced. The combined Chinese medicine enables them to recover quickly after childbirth, has antibacterial and anti-inflammatory effects and promotes lactation, and the immunity has been improved, resulting in increased milk yield, milk fat rate, and milk protein rate;

[0128] Compared with Comparative Example 2, the milk yield, milk fat rate, and milk protein rate in Example 2 have also increased significantly. This is because in the present invention, the mycelium Chinese medicine compound is increased by 20% in the concentrate supplement, and the nutritional components such as crude protein have increased. When the mycelium protein is digested and fermented in the rumen of buffaloes, acetic acid is generated. After being absorbed, the acetic acid is transported to the mammary gland through the blood and used to synthesize some short-chain fatty acids in milk fat, ultimately increasing the milk fat rate of buffalo milk;

[0129] Compared with Examples 3 - 4, the milk yield, milk fat percentage, milk protein percentage, and lactose in Example 5 are also significantly increased. This is because in the present invention, the content of dried papaya, tetrapanax papyrifer, honeysuckle, and forsythia in the mycelial protein is increased. While increasing the protein, the synergistic effect of various traditional Chinese medicine components is achieved. In particular, papaya has a certain inhibitory effect on various bacteria and viruses, such as antibacterial activity against Escherichia coli, Staphylococcus aureus, etc., and also has a certain inhibitory effect on the enterovirus of milk buffalo, which helps to prevent and reduce related infectious diseases. Papain, lipase, etc. contained in papaya can promote the decomposition of protein and fat, which is helpful for the digestion and absorption of milk buffalo, and can improve indigestion, loss of appetite, etc. Components such as akebioside in tetrapanax papyrifer play an important role in exerting the effects of clearing heat and promoting diuresis, promoting lactation, etc., and can effectively promote the milk secretion of milk buffalo. Honeysuckle has an inhibitory effect on various pathogenic bacteria such as Staphylococcus aureus, hemolytic streptococcus, Escherichia coli, Shigella dysenteriae, Vibrio cholerae, and Salmonella typhi, can promote the release of adrenal cortical hormones, has an inhibitory effect on inflammation, and can protect the body of milk buffalo from disease, anti - inflammation, disease resistance, postpartum repair, and health. Long - term feeding of traditional Chinese medicine in the concentrate supplement can ensure the health and stability of milk buffalo.

[0130] Compared with Comparative Examples 3 - 5, the milk yield, milk fat percentage, and milk protein percentage in Example 5 are also increased. This is because in the present invention, the mycelial protein is compounded with a variety of effective traditional Chinese medicines, which has antioxidant properties and is a prerequisite for the synthesis of various hormones, vitamin D, and steroids. It can significantly enhance protein synthesis and precipitation and improve the immune ability of milk buffalo;

[0131] In summary, feeding lactating milk buffalo with the feed prepared by the feed formula of the present invention has comprehensive nutrition, improves the immunity of milk buffalo, has a low incidence rate, increases the milk yield during the lactation period, and improves the milk fat percentage, milk protein percentage, and selenium content of the produced buffalo milk.

Claims

1. A method for preparing citrus citrus fungus rod material by mixing mulberry and laba beans as feed, characterized in that: The following steps are involved: (1) Pretreatment of mulberry and Labrador bean mixed micro-stock: The mulberry and Laba beans are crushed separately by a crusher, and the crushed particles have a diameter of ≤2.5 cm; the mulberry and Laba beans are uniformly mixed according to a certain weight ratio, corn husks are sprayed as a moisture regulator and fermentation substrate, the moisture content of the mixed micro-storage is adjusted to 50%-60%, glucose, saccharifying enzyme, yeast and fermentation bacteria are added to the mixed micro-storage in a certain weight ratio, and the mixed micro-storage is uniformly stirred and mixed, the silage is bagged and sealed, and fermented at room temperature of 25°C-30°C to obtain a pretreated material; (2) Preparation of Pleurotus citrinopileatus culture medium based on mixed micro-stock of mulberry and Labrador beans: Weigh the pretreated material, jasmine flower residue, bagasse, barley husk, corn flour, calcium carbonate, magnesium sulfate, sugar and gypsum powder in step (1) according to a certain proportion; weigh according to the formula ratio, sugar water and magnesium sulfate should be dissolved in water first, mix the materials evenly, and the moisture content of the culture medium is 60%-65%; the prepared culture medium should be piled and stuffed for 2-4 hours to allow the culture medium to absorb enough water evenly to prevent the material from being unevenly dry and wet; when bagging, gently compact the material while filling it, so that the bag wall is smooth and has no gaps around it to prevent mushrooms from growing around the bag; when the material is filled to a certain height, flatten the surface of the material and seal it by folding; sterilize the culture medium immediately after filling it; use normal pressure sterilization, when the material bag is loaded into the sterilization furnace, the material surface is facing up, the bags are stacked on top of each other, and there is a slight gap between the bags; keep the temperature for 10-12 hours and stop heating after the material temperature reaches a certain temperature; (3) Activating the strain: Inoculate the slant of Pleurotus citrinopileatus under a sterile environment, and place it in a liquid culture medium for shake flask culture; (4) Biological transformation of Pleurotus citrinopileatus: the liquid strain in the liquid culture medium is placed in the Pleurotus citrinopileatus culture medium, and the mixture is bagged and cultured. After the inoculation, the mixture is placed in a culture room at a certain temperature for fermentation, and a Pleurotus citrinopileatus culture stick is obtained after the mycelium fills the bag. Specifically, the liquid strain and the Pleurotus citrinopileatus culture medium are inoculated according to a certain mass and cultured at a suitable temperature, and a Pleurotus citrinopileatus culture stick is obtained after the mycelium grows to fill the bag for several days; (5) Preparation of Pleurotus citrinopileatus spawn stick material: The cultivated Pleurotus citrinopileatus spawn sticks are dried using a drying technology, first by hot air drying and then by hot air furnace treatment, to preserve the original nutrients unchanged, thereby obtaining Pleurotus citrinopileatus spawn stick material.

2. The method for preparing citrus citrus fungus stick material by mixing mulberry and laba beans as feed according to claim 1, characterized in that: The weight ratio of the feed mulberry and Laba beans in step (1) is 2:

1.

3. The method for preparing citrus citrus fungus rod material by mixing mulberry and labrador bean feed micro-stock according to claim 1, characterized in that: The weight ratio of glucose, saccharifying enzyme, yeast and fermentation bacteria in step (1) is 4:1:0.5:0.

5.

4. The method for preparing citrus citrus fungus stick material by mixing mulberry and laba beans as feed according to claim 1, characterized in that: The silage in step (1) is bagged, sealed, and fermented at room temperature (25°C-30°C) for 20 days to obtain a pretreated material.

5. The method for preparing citrus citrus fungus rod material by mixing mulberry and laba beans as feed according to claim 1, characterized in that: In step (2), 70% of the pretreated material of step (1), 5% of jasmine dry residue, 9% of bagasse, 10% of barley husk, 4% of corn flour, 0.2% of calcium carbonate, 0.3% of magnesium sulfate, 1% of sugar and 0.5% of gypsum powder are weighed.

6. The method for preparing citrus citrus fungus rod material by mixing mulberry and labrador bean feed micro-stock according to claim 1, characterized in that: The conditions for hot air drying in step (5) are: temperature of 100° C., single layer laying, and treatment for 20 minutes.

7. The method for preparing citrus citrus fungus rod material by mixing mulberry and laba beans as feed according to claim 1, characterized in that: The conditions of the hot air furnace treatment in step (5) are: drying at 200° C.-250° C. for 30 minutes.

8. An application of the citrus pleurotus eryngii mushroom stick material prepared according to the method according to any one of claims 1 to 7 in preparing a traditional Chinese medicine composite material for dairy buffaloes, the traditional Chinese medicine composite material for dairy buffaloes comprising the following raw materials in parts by weight: 850-950 parts of citrus pleurotus eryngii mushroom stick material, 150-250 parts of dried papaya, 200-300 parts of dried water spinach stalks, 100-200 parts of yam residue, 80-90 parts of astragalus, 80-90 parts of liquorice, 40-60 parts of tongcao, 20-40 parts of dandelion, 60-80 parts of honeysuckle, and 40-60 parts of forsythia.

9. Use of the citrus pleurotus eryngii mushroom stick material prepared by the method according to claim 8 in preparing a traditional Chinese medicine composite material for dairy buffaloes, wherein the traditional Chinese medicine composite material for dairy buffaloes comprises the following raw materials in parts by weight: 950 parts of citrus pleurotus eryngii mushroom stick material, 250 parts of dried papaya, 200 parts of yam residue, 300 parts of dried water spinach stalks, 90 parts of astragalus, 90 parts of licorice, 60 parts of tongcao, 40 parts of dandelion, 80 parts of honeysuckle, and 60 parts of forsythia.

10. Use of the citrus pulverulosus mushroom stick material prepared by the method according to claim 8 or 9 in preparing a traditional Chinese medicine composite material for dairy buffaloes, wherein the traditional Chinese medicine in the traditional Chinese medicine composite material for dairy buffaloes is all dry, crushed to 80 meshes, and then evenly stirred and mixed with the citrus pulverulosus mushroom stick material.

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