An anti-wrinkle composition, its preparation method, application, and cosmetics.

Abstract

This application belongs to the field of cosmetic manufacturing technology and discloses an anti-wrinkle composition and its preparation method, application, and cosmetics, including Magnolia officinalis flower extract and Phyllanthus emblica fruit extract, wherein the mass ratio of Magnolia officinalis flower extract to Phyllanthus emblica fruit extract is 0.1-8:0.5-10; through the synergistic effect between Magnolia officinalis flower extract and Phyllanthus emblica fruit extract, the composition has both good elastase inhibition ability and excellent antioxidant ability, so that the composition has good elastase inhibition ability and good free radical scavenging ability, thereby achieving good anti-wrinkle performance.

Description

Technical Field

[0001] This application relates to the field of cosmetic manufacturing technology, and in particular to an anti-wrinkle composition, its preparation method, application, and cosmetics. Background Technology

[0002] Elastase is a protease released by inflammatory cells such as neutrophils and macrophages. Its main function is to break down elastin in the extracellular matrix. Elastin is an important structural protein in the dermis, giving the skin elasticity, firmness, and resilience. With age, the skin's self-repair ability declines, elastin synthesis decreases, and its breakdown increases, leading to skin laxity and wrinkle formation. By inhibiting elastase activity, its degradation of elastin is reduced, maintaining skin elasticity and firmness can be achieved. Furthermore, preserving elastin can reduce fine lines and deep wrinkles caused by structural collapse.

[0003] Free radicals (such as reactive oxygen species (ROS) and reactive nitrogen species (RNS)) are highly oxidizing molecules produced by metabolic products or external stimuli. They trigger oxidative stress by attacking lipids, proteins, and DNA within cells. Free radicals can directly damage collagen and elastin fibers, causing the skin to lose its support. Simultaneously, mitochondrial DNA damage and cell membrane lipid peroxidation accelerate cell apoptosis or aging. Neutralizing free radicals can protect collagen, elastin, and cell membrane structure, delaying skin sagging and wrinkles.

[0004] Prior art 1: Chinese patent application 202010850281.5 discloses a plant-derived anti-aging composition and its application. The composition contains NMN and plant extracts, wherein the plant extracts are any one or more of rosemary extract, dewwort extract, *Anoectochilus roxburghii* extract, and *Phyllanthus emblica* extract.

[0005] Further examination of the instructions reveals that the formula states that Phyllanthus emblica extract can significantly increase the activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in serum and tissues, and significantly reduce the levels of malondialdehyde (MAD) and lipoprotein (LPF). Phyllanthus emblica extract contains a large amount of polyphenols, exhibiting significant antioxidant, anti-inflammatory, antibacterial, antiviral, and anti-aging effects.

[0006] Prior art 2: Chinese patent application 202410362678.8 discloses a method for extracting magnolia flower, the extract and its products. Further observation of the specification of this solution reveals that the magnolia flower extract has a certain soothing ability through testing, and also has a certain firming ability. Furthermore, it is verified that the magnolia flower extract has a certain anti-wrinkle effect.

[0007] In summary, existing technologies have disclosed that both Phyllanthus emblica and Magnolia officinalis possess certain anti-aging abilities, with Phyllanthus emblica's anti-aging mechanism primarily stemming from its excellent antioxidant mechanism.

[0008] While Magnolia denudata exhibits certain firming and anti-wrinkle properties, current technology does not disclose its antioxidant properties.

[0009] More importantly, existing technologies have not explored whether mixing Magnolia officinalis flowers with Phyllanthus emblica fruit still provides good anti-wrinkle properties.

[0010] The problem this solution aims to solve is: how to provide an anti-wrinkle composition with good firming ability. Summary of the Invention

[0011] The purpose of this application is to provide an anti-wrinkle composition that, through the synergistic effect of Magnolia officinalis flowers and Phyllanthus emblica fruits, exhibits both excellent elastase inhibitory ability and superior antioxidant capacity. This results in a composition with both good elastase inhibitory ability and good free radical scavenging ability. Since free radicals damage collagen and elastin fibers, leading to skin laxity and accelerated wrinkle formation, antioxidant components can neutralize free radicals and reduce their damage to skin structure. Therefore, the anti-wrinkle composition provided in this application aims to achieve anti-wrinkle effects by inhibiting elastase and scavenging free radicals.

[0012] To achieve the above objectives, this application discloses an anti-wrinkle composition comprising Magnolia officinalis flower extract and Phyllanthus emblica fruit extract, wherein the mass ratio of Magnolia officinalis flower extract to Phyllanthus emblica fruit extract is 0.1–8:0.5–10.

[0013] When Magnolia officinalis flower extract and Phyllanthus emblica fruit extract are used together, on the one hand, their excellent free radical scavenging ability reduces oxidative stress at the source and inhibits the release of elastase; on the other hand, because both have good elastase inhibition ability, they block the damage to elastin at the end, forming a double defense and further enhancing the anti-wrinkle ability of the composition.

[0014] Preferably, it also includes clove flower extract, wherein the mass ratio of the magnolia flower extract, amla fruit extract, and clove flower extract is 0.1-8:0.5-10:0.5-14.

[0015] Clove flower extract is a mixture of various components, some of which can interact with elastase. Certain terpenoid compounds it contains can inhibit the hydrolytic action of elastase on elastic fibers.

[0016] In addition, this application also discloses a method for preparing the above-mentioned anti-wrinkle composition, wherein extracts of Magnolia officinalis flower and Phyllanthus emblica fruit are mixed and dispersed to obtain the anti-wrinkle composition.

[0017] Preferably, the extraction method of the Magnolia officinalis flower extract is as follows: the Magnolia officinalis flowers are dried, then placed in an alcohol solvent and heated and stirred, and centrifuged to obtain an intermediate, and then the intermediate is immersed in a fat-soluble solvent, stirred, extracted and centrifuged to obtain the Magnolia officinalis flower extract.

[0018] The extraction method of the amla fruit extract is as follows: the amla fruit is dried, then placed in an alcohol solvent and heated and stirred, centrifuged to obtain an intermediate, then the intermediate is immersed in a fat-soluble solvent, stirred, extracted and centrifuged to obtain the amla fruit extract.

[0019] The extraction method of clove flower extract is as follows: dry clove flowers, then place them in an alcohol solvent, heat and stir, centrifuge to obtain an intermediate, then immerse the intermediate in a fat-soluble solvent, stir, extract and centrifuge to obtain clove flower extract.

[0020] Preferably, the fat-soluble solvent is selected from at least one of glycerol tri(ethylhexanoate), caprylic / capric triglyceride, octyl dodecanol, cocoyl octanoate / capric ester, ethylhexyl triazine, cetyl ethylhexanoate, and meadowfoam seed oil.

[0021] In addition, this application also discloses the use of the anti-wrinkle composition as described above in the preparation of cosmetics.

[0022] In addition, this application also discloses a cosmetic containing 0.1 to 20 wt% of the anti-wrinkle composition as described above.

[0023] Preferably, the dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion, or cream.

[0024] Preferably, the cosmetic is a gel, essential oil, face mask, facial cleanser, lipstick, or blush.

[0025] The beneficial effects of this application are:

[0026] When Magnolia officinalis flower extract and Phyllanthus emblica fruit extract are used together, on the one hand, their excellent free radical scavenging ability reduces oxidative stress at the source and inhibits the release of elastase; on the other hand, because both have good elastase inhibition ability, they block the damage to elastin at the end, forming a double defense and further enhancing the anti-wrinkle ability of the composition. Attached Figure Description

[0027] Figure 1 This is a chicken embryo image of the sample corresponding to Example 5 before testing during the irritation test;

[0028] Figure 2 This is a chicken embryo image of the sample corresponding to Example 5 after testing during the irritation test. Detailed Implementation

[0029] The present application will be clearly and completely described below with reference to its embodiments. It should be noted that, unless specific conditions are specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0030] Before demonstrating the embodiments, the preparation and acquisition methods of the raw materials involved in the embodiments shall be explained as follows:

[0031] Magnolia officinalis: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0032] Phyllanthus emblica fruit: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0033] Clove: Bozhou Yuanshengtang Pharmaceutical Co., Ltd.;

[0034] Ethanol: Guangxi Jinyuan Biochemical Industry Co., Ltd., purity over 95%;

[0035] Triglyceride (TIO): Guangzhou Hemiao Biotechnology Co., Ltd.

[0036] Preparation method of anti-wrinkle composition (unless otherwise specified, the compositions in the following examples and comparative examples are all prepared by this method):

[0037] Extraction method of Magnolia officinalis flower extract:

[0038] The flowers of Magnolia officinalis were dried and crushed. Then, the flowers were immersed in a 95% ethanol solution and stirred at 40°C for 3 hours. After centrifugation, the ethanol solution was heated at 65°C to accelerate its evaporation, and an intermediate was obtained. It should be noted that the amount of ethanol solution added should be sufficient to completely submerge the crushed flowers. In the actual operation of this case, the mass ratio between the crushed flowers and the ethanol solution was 1:25.

[0039] The intermediate was then immersed in TIO solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding the Magnolia officinalis flower extract. It should also be noted that the amount of TIO added should be sufficient to completely submerge the intermediate. In the actual operation of this case, the mass ratio of the intermediate to TIO was 1:35.

[0040] Extraction method of Phyllanthus emblica fruit extract:

[0041] The amla fruit was dried and crushed, then immersed in a 95% ethanol solution at 40°C for 3 hours and stirred. After centrifugation, the ethanol solution was heated at 55°C to evaporate, yielding an intermediate. It should be noted that the amount of ethanol solution added should be sufficient to completely submerge the crushed amla fruit. In the actual operation of this case, the mass ratio between the crushed amla fruit and the ethanol solution was 1:30.

[0042] The intermediate was then immersed in TIO solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding Phyllanthus emblica fruit extract. It should also be noted that the amount of TIO added should be sufficient to completely submerge the intermediate. In the actual operation of this case, the mass ratio of intermediate to TIO was 1:30.

[0043] Extraction method of clove flower extract:

[0044] The clove flowers were dried and crushed, and then immersed in a 95% ethanol solution at 40°C for 3 hours. After that, the mixture was centrifuged and heated at 60°C to evaporate the ethanol solution, thus obtaining an intermediate. It should be noted that the amount of ethanol solution added should be sufficient to completely submerge the crushed clove flowers. In the actual operation of this case, the mass ratio between the crushed clove flowers and the ethanol solution was 1:20.

[0045] The intermediate was then immersed in TIO solvent, stirred and extracted for 1 hour, and then centrifuged to remove insoluble substances to obtain clove extract. It should also be noted that the amount of TIO added should be sufficient to completely submerge the intermediate. In the actual operation of this case, the mass ratio of intermediate to TIO was 1:25.

[0046] The anti-wrinkle composition is prepared by mixing and dispersing the extracts of Magnolia officinalis flower and Phyllanthus emblica fruit to obtain the anti-wrinkle composition.

[0047] Examples 1-6

[0048] An anti-wrinkle composition, the raw materials and their mass ratios are shown in Table 1:

[0049] Table 1: Formulation table of compositions in Examples 1-6

[0050]

[0051] Comparative Examples 1-5

[0052] A composition, the raw materials and their mass ratios are shown in Table 2:

[0053] Table 2: Formulation table of compositions for Comparative Examples 1-5

[0054]

[0055]

[0056] Performance testing:

[0057] 1. Stimulation test

[0058] 1.1 Instruments and Equipment

[0059] Fully automatic incubator, stereo microscope, SPF chicken embryos;

[0060] 1.2 Reagents

[0061] Sodium chloride, sodium dodecyl sulfate (SDS);

[0062] 1.3 Incubation conditions

[0063] Room temperature 20℃~25℃, relative humidity 45%~70%, incubation temperature 37.5℃±0.5℃, relative humidity 55%~70%, turntable 3 to 6 times / h, no rotation is required when incubating 9-day-old chicken embryos.

[0064] 1.4 Test Methods

[0065] (1) Experimental operation procedures

[0066] In this test, six embryos were selected for each group. The condition of the chorioallantoic membrane was recorded using a photographic device. A polytetrafluoroethylene (PTFE) resin ring was placed on the chorioallantoic membrane of the chicken embryo, and the image was taken and recorded. The anti-wrinkle composition prepared in Example 5 was diluted with peanut oil to a 2% (w / w) oil solution and used as the test sample. The test sample was then added to the PTFE resin ring, and the time of sample addition was recorded. The air cell was covered with a moistened plastic wrap, and the chicken embryos were transferred to a constant temperature and humidity incubator for cultivation. The degree of change in each toxicity effect was observed.

[0067] (2) Results observation

[0068] Observe and record bleeding, coagulation, and vascularization manifestations, and score them according to their severity.

[0069] The scoring criteria for bleeding, coagulation, and vascularization are shown in Table 3:

[0070] Table 3: Scoring Criteria for Bleeding, Coagulation, and Vascular Dissolution

[0071]

[0072] Scoring criteria: ES≤12: no / mild irritation; 12<ES<16: moderate irritation; ES≥16: strong irritation.

[0073] refer to Figure 1-2Upon testing, the ES value of the sample corresponding to Example 5 was 2.00, which is less than 12, indicating no irritation.

[0074] 2. Elastase inhibition rate test

[0075] 2.1 Instruments and Equipment

[0076] BSA224S analytical balance, RT-6100 enzyme-linked immunosorbent assay (ELISA) analyzer;

[0077] 2.2 Reagents

[0078] Elastase (porcine pancreas), BR, N-succinyl-L-alanyl-L-alanyl-L-alanine, 98%;

[0079] 2.3 Test Methods

[0080] (1) Treatment of control materials and test samples

[0081] Sample group: Dilute the samples (compositions prepared in the examples and comparative examples) with ethanol to a mass fraction of 5%;

[0082] Negative control: ethanol.

[0083] (2) Experimental Operation Procedures

[0084] Set up a sample group, a sample background group, a solvent group, and a solvent background group. Each group should be set up in 3 replicates. Add different reagent solutions to 96-well plates, shake gently, incubate at 25°C for 15 min, and then place them in an ELISA reader to measure the absorbance at 410 nm.

[0085] (3) The calculation formula is shown in Equation 1:

[0086]

[0087] In the formula: A—is the absorbance of the reaction solution without the sample and the reaction solution containing the enzyme;

[0088] B—The absorbance of the reaction solution without the sample and enzyme;

[0089] C—is the absorbance of the reaction solution containing the sample and enzyme;

[0090] D—The absorbance of the reaction solution containing the sample and the reaction solution without the enzyme.

[0091] The test results are shown in Table 4:

[0092] Table 4: Results of Elastase Inhibition Rate Test

[0093]

[0094]

[0095] Results analysis:

[0096] 1. As can be seen from Examples 1-3, when the mass ratio between the Magnolia officinalis flower extract and the Phyllanthus emblica fruit extract is slightly adjusted, the elastase inhibition rate of Examples 1-3 shows a certain degree of fluctuation. Furthermore, when the mass ratio between the Magnolia officinalis flower extract and the Phyllanthus emblica fruit extract in Example 2 is 4:4, the composition undoubtedly has a more superior elastase inhibition ability.

[0097] Further observation of Examples 4-6 revealed that, based on Example 2, clove flower extract was further introduced in Examples 4-6. When the mass ratio of Magnolia officinalis flower extract, Phyllanthus emblica fruit extract, and clove flower extract was 4:4:4, the elastase inhibitory ability of the composition was most significantly improved.

[0098] 2. As can be seen from Example 2 and Comparative Examples 1-2, when the compositions of Comparative Examples 1-2 contain only Magnolia officinalis flower extract or Phyllanthus emblica fruit extract, the elastase inhibition rates of Comparative Examples 1-2 are only 23.57% and 27.97%, respectively.

[0099] When the composition of Example 2 consisted of a mixture of Magnolia officinalis flower extract and Phyllanthus emblica fruit extract in a mass ratio of 4:4, the elastase inhibition rate of Example 2 reached 35.14%, which was much higher than that of Comparative Examples 1 and 2.

[0100] It is evident that there is a synergistic effect between the extract of Magnolia officinalis flower and the extract of Phyllanthus emblica fruit, which significantly enhances the elastase inhibitory ability of the composition prepared in Example 2.

[0101] 3. As can be seen from Example 5, Comparative Examples 1-2 and Comparative Example 5, when the compositions of Comparative Examples 1-2 contain only Magnolia officinalis flower extract or Phyllanthus emblica fruit extract, the elastase inhibition rates of Comparative Examples 1-2 are only 23.57% and 27.97%, respectively.

[0102] When the composition of Comparative Example 5 contained only clove flower extract, the elastase inhibition rate of Comparative Example 5 was 25.24%; while when Example 5 was composed of Magnolia officinalis flower extract, Phyllanthus emblica fruit extract, and clove flower extract, its elastase inhibition rate reached 41.29%. Based on the elastase inhibition ability of the compositions of Comparative Examples 1-2 and Comparative Example 5, the theoretical value of the elastase inhibition rate of Example 5 should be (23.57% + 27.97% + 25.24%) / 3 = 25.59%.

[0103] It can be seen that the elastase inhibition rate of Example 5 exceeded its theoretical value, that is, the elastase inhibition ability of the composition prepared in Example 5 exceeded the simple superposition of Comparative Examples 1-2 and Comparative Example 5.

[0104] This demonstrates a synergistic effect among the extracts of Magnolia officinalis flower, Phyllanthus emblica fruit, and Syringa vulgaris flower;

[0105] Further observation of Comparative Examples 3-4 revealed that when Magnolia officinalis flower extract or Phyllanthus emblica fruit extract was used in combination with Clove flower extract, the elastase inhibition rate of Comparative Example 3 was 24.20%, and its ability to inhibit elastase was between that of Comparative Examples 1 and 5. It can be seen that the Magnolia officinalis flower extract and Clove flower extract in Comparative Example 3 did not produce a significant synergistic effect.

[0106] The elastase inhibition rate of Comparative Example 4 reached 30.02%, which was significantly higher than that of Comparative Example 2 and Comparative Example 5. This indicates that there is a relatively strong synergistic effect between Phyllanthus emblica fruit extract and Clove flower extract in Comparative Example 4.

[0107] In summary, there is a synergistic effect between the extract of Magnolia officinalis flower and the extract of Phyllanthus emblica fruit;

[0108] 1. There is a synergistic effect between Phyllanthus emblica fruit extract and Clove flower extract, but the synergistic effect is relatively weaker than that between Phyllanthus emblica fruit extract and Magnolia officinalis flower extract;

[0109] 2. The synergistic effect of clove flower extract and magnolia flower extract is not strong when used together, but when amla fruit extract is added to the composition, the synergistic effect of the three can be more fully exerted.

[0110] 3. DPPH free radical scavenging rate test

[0111] 3.1 Instruments and Equipment

[0112] BSA224S analytical balance, L6s ultraviolet spectrophotometer

[0113] 3.2 Reagents

[0114] DPPH (1,1-diphenyl-2-picrylhydrazine), 98%

[0115] 3.3 Test Methods

[0116] (1) Processing of control materials and test samples

[0117] Sample group: Dilute the samples (compositions prepared in the examples and comparative examples) with ethanol to a mass fraction of 5%;

[0118] Negative control: ethanol.

[0119] (2) Experimental Operation Procedures

[0120] Set up a sample group and a negative control group, with three parallel tubes for each group. Add the corresponding reagent solution to each group, shake gently, and let stand at room temperature for 5 minutes. Transfer the reaction solution of each group into a 1 cm cuvette and measure the absorbance at 517 nm.

[0121] (3) The calculation formula is shown in Equation 2:

[0122]

[0123] Where: T—absorbance of the sample tube, i.e., absorbance of the solution after the sample reacts with DPPH;

[0124] T0—Sample background absorbance;

[0125] The average of three absorbance values ​​of the C-DPPH tube, i.e. the absorbance of the DPPH solution without the addition of sample;

[0126] C0 — Solvent background absorbance.

[0127] The test results are shown in Table 5:

[0128] Table 5: DPPH Free Radical Scavenging Rate Test Results

[0129]

[0130]

[0131] Results analysis:

[0132] 1. As can be seen from Examples 1-3, when the mass ratio between the Magnolia officinalis flower extract and the Phyllanthus emblica fruit extract is slightly adjusted, the free radical scavenging rate of Examples 1-3 shows a certain degree of fluctuation. Furthermore, when the mass ratio between the Magnolia officinalis flower extract and the Phyllanthus emblica fruit extract in Example 2 is 4:4, the composition undoubtedly has a more superior free radical scavenging ability.

[0133] Further observation of Examples 4-6 revealed that, based on Example 2, clove flower extract was further introduced in Examples 4-6. The free radical scavenging ability of the composition was most significantly improved when the mass ratio of Magnolia officinalis flower extract, Phyllanthus emblica fruit extract, and clove flower extract was 4:4:4.

[0134] 2. As can be seen from Example 2 and Comparative Examples 1-2, when the compositions of Comparative Examples 1-2 contain only Magnolia officinalis flower extract or Phyllanthus emblica fruit extract, the free radical scavenging rates of Comparative Examples 1-2 are only 33.39% and 34.38%, respectively.

[0135] When the composition of Example 2 consisted of a mixture of Magnolia officinalis flower extract and Phyllanthus emblica fruit extract in a mass ratio of 4:4, the free radical scavenging rate of Example 2 reached 42.10%, which was much higher than that of Comparative Examples 1 and 2.

[0136] It is evident that there is a synergistic effect between the extract of Magnolia officinalis flower and the extract of Phyllanthus emblica fruit, which significantly enhances the free radical scavenging ability of the composition prepared in Example 2.

[0137] 3. As can be seen from Example 5, Comparative Examples 1-2 and Comparative Example 5, when the compositions of Comparative Examples 1-2 contain only Magnolia officinalis flower extract or Phyllanthus emblica fruit extract, the free radical scavenging rates of Comparative Examples 1-2 are only 33.39% and 34.38%, respectively.

[0138] When the composition of Comparative Example 5 contained only clove flower extract, the free radical scavenging rate of Comparative Example 5 was 38.62%; while when Example 5 was composed of Magnolia officinalis flower extract, Phyllanthus emblica fruit extract and clove flower extract, its free radical scavenging rate reached 50.61%. Based on the free radical scavenging ability of the compositions of Comparative Examples 1-2 and Comparative Example 5, the theoretical value of the free radical scavenging rate of Example 5 should be (33.39% + 34.38% + 38.62%) / 3 = 35.46%.

[0139] It is evident that the free radical scavenging rate of Example 5 exceeded its theoretical value, meaning that the free radical scavenging ability of the composition prepared in Example 5 exceeded the simple summation of Comparative Examples 1-2 and Comparative Example 5.

[0140] This demonstrates a synergistic effect among the extracts of Magnolia officinalis flower, Phyllanthus emblica fruit, and Syringa vulgaris flower;

[0141] Further observation of Comparative Examples 3-4 shows that when Magnolia officinalis flower extract or Phyllanthus emblica fruit extract was used in combination with Clove flower extract, the free radical scavenging rate of Comparative Example 3 was 37.16%, which was higher than that of Comparative Examples 1 and 5. It can be seen that the Magnolia officinalis flower extract and Clove flower extract produced a relatively obvious synergistic effect in Comparative Example 3.

[0142] The free radical scavenging rate of Comparative Example 4 reached 35.95%, which is between that of Comparative Example 2 and Comparative Example 5. It can be seen that the synergistic effect between Phyllanthus emblica fruit extract and Clove flower extract in Comparative Example 4 is very weak in the direction of free radical scavenging.

[0143] In summary, there is a synergistic effect between the extract of Magnolia officinalis flower and the extract of Phyllanthus emblica fruit;

[0144] 1. There is a synergistic effect between Magnolia officinalis flower extract and Syzygium aromaticum flower extract, but the synergistic effect is relatively weaker than that between Phyllanthus emblica fruit extract and Magnolia officinalis flower extract;

[0145] 2. The synergistic effect of clove flower extract and amla fruit extract is weak when used together, but the synergistic effect of the three can be more fully exerted when magnolia flower extract is added to the composition.

[0146] Application examples

[0147] An anti-wrinkle product, the formula of which is shown in Table 6:

[0148] Table 6: Formulation of Anti-wrinkle Products

[0149]

[0150]

[0151] An anti-wrinkle product, the formula is shown in Table 6, and the specific preparation method is as follows:

[0152] Step 1: Preheat phase A to 80-85℃ to dissolve, stir and mix evenly, and set aside.

[0153] Step 2: Add the ingredients from phase B into the main pot in sequence and stir well.

[0154] Step 3: Add phase A and phase C, stir well, and it's ready.

[0155] The embodiments presented herein are merely selected implementations based on combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Some numerical ranges used in the claims include sub-ranges within them, and variations within these ranges should also be covered by the appended claims.

Claims

1. An anti-wrinkle composition, characterized in that, It is composed of extracts of Magnolia officinalis flower and Phyllanthus emblica fruit, wherein the mass ratio of Magnolia officinalis flower extract to Phyllanthus emblica fruit extract is 0.1-8:0.5-10; The extraction method of the Magnolia officinalis flower extract is as follows: the Magnolia officinalis flowers are dried and crushed, then the Magnolia officinalis flowers are immersed in a 95% ethanol solution and stirred at 40°C for 3 hours, then centrifuged, and heated at 65°C to accelerate the evaporation of the ethanol solution to obtain the intermediate. The intermediate was then immersed in a fat-soluble solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding the Magnolia officinalis flower extract. The extraction method of the amla fruit extract is as follows: the amla fruit is dried and crushed, then the amla fruit is soaked in a 95% ethanol solution and stirred at 40°C for 3 hours, then centrifuged, and heated at 55°C to evaporate the ethanol solution to obtain the intermediate. The intermediate was then immersed in a fat-soluble solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding Phyllanthus emblica fruit extract.

2. An anti-wrinkle composition, characterized in that, It is composed of extracts of Magnolia officinalis flower, Phyllanthus emblica fruit, and Clove flower, wherein the mass ratio of the extracts of Magnolia officinalis flower, Phyllanthus emblica fruit, and Clove flower is 0.1-8:0.5-10:0.5-14. The extraction method of the Magnolia officinalis flower extract is as follows: the Magnolia officinalis flowers are dried and crushed, then the Magnolia officinalis flowers are immersed in a 95% ethanol solution and stirred at 40°C for 3 hours, then centrifuged, and heated at 65°C to accelerate the evaporation of the ethanol solution to obtain the intermediate. The intermediate was then immersed in a fat-soluble solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding the Magnolia officinalis flower extract. The extraction method of the amla fruit extract is as follows: the amla fruit is dried and crushed, then the amla fruit is soaked in a 95% ethanol solution and stirred at 40°C for 3 hours, then centrifuged, and heated at 55°C to evaporate the ethanol solution to obtain the intermediate. The intermediate was then immersed in a fat-soluble solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding Phyllanthus emblica fruit extract. The extraction method of the clove flower extract is as follows: the clove flowers are dried and crushed, then the clove flowers are soaked in a 95% ethanol solution and stirred at 40°C for 3 hours, then centrifuged, and heated at 60°C to evaporate the ethanol solution to obtain the intermediate. The intermediate was then immersed in a fat-soluble solvent, stirred and extracted for 1 hour, followed by centrifugation to remove insoluble substances, yielding clove flower extract.

3. A method for preparing the anti-wrinkle composition according to claim 1, characterized in that, The extracts of Magnolia officinalis flower and Phyllanthus emblica fruit were mixed and dispersed to obtain an anti-wrinkle composition.

4. The method for preparing the anti-wrinkle composition according to claim 3, characterized in that, The fat-soluble solvent is selected from at least one of the following: triglyceride (ethylhexanoate), caprylic / capric triglyceride, octyl dodecanol, cocoyl octanoate / capric ester, ethylhexyl triazine, cetyl ethylhexanoate, and meadowfoam seed oil.

5. Use of the anti-wrinkle composition according to any one of claims 1-2 in the preparation of cosmetics.

6. A cosmetic product, characterized in that, Containing 0.1 to 20 wt% of the anti-wrinkle composition as described in any one of claims 1-2.

7. The cosmetic product according to claim 6, characterized in that, The dosage form of the cosmetic is a patch, ointment, liquid, spray, gel, emulsion or cream.

8. The cosmetic product according to claim 6, characterized in that, The cosmetics mentioned are gels, essential oils, face masks, facial cleansers, lipsticks, or blushes.

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