Method for preparing nanogold compound for treating bladder cancer based on curcumin

Abstract

The invention relates to a method for treating bladder cancer by preparing a nanogold compound based on curcumin, and aims to prepare and modify nanogold particles for treating bladder cancer. Firstly, gold nanoparticles are synthesized in an aqueous solution through a reduction reaction, and the particle size and the surface property are controlled; then, through introduction of a surfactant, surface modification is carried out on the nano-gold particles, and the biocompatibility and the stability of the nano-gold particles are enhanced. Next, curcumin is dissolved by adopting an organic solvent, such as dimethyl sulfoxide (DMSO), and the curcumin is introduced into the nanogold solution, so that further modification on the nanogold is realized; various characterization technologies including a transmission electron microscope, an ultraviolet-visible absorption spectrum, a Fourier transform infrared spectrum and the like are used in the experiment process, and the morphology and the structure of the gold nanoparticles are analyzed in detail. Finally, the obtained modified gold nanoparticles have good biocompatibility and stability, and a new scheme is provided for further application in bladder cancer treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of nanomaterials, and particularly to a method for preparing a nano-gold complex based on curcumin for the treatment of bladder cancer. Background Art

[0002] Bladder cancer has a high tendency of recurrence and metastasis. Even after initial treatment, the cancer may reappear or metastasize to other parts. This leads to the need for long-term follow-up and monitoring of patients. Traditional treatments for bladder cancer include surgical removal of the bladder, which has a significant impact on the quality of life of patients. After surgery, patients may need to undergo postoperative management of urinary bags, which poses challenges to both their physical and mental health. Chemotherapy and radiotherapy are common treatment methods for bladder cancer, but they may cause a series of side effects, such as nausea, fatigue, immune system suppression, etc. These side effects may have a negative impact on the quality of life and treatment compliance of patients. Some bladder cancers may develop drug resistance to chemotherapy drugs, weakening the treatment effect. This makes the search for new treatment methods particularly urgent.

[0003] Ordinary nano-gold particles may cause problems of biocompatibility and toxicity. In treatment, it is necessary to ensure that nano-gold has less toxicity to normal cells while having sufficient selectivity for cancer cells. The distribution of nano-gold in the body may be affected by various factors, including blood circulation, tissue structure, etc. Ensuring that nano-gold can accurately target bladder cancer cells without affecting healthy tissues is a challenging problem. For the treatment effect of ordinary nano-gold, especially for complex tumors such as bladder cancer, there may be uncertainties. The effectiveness of the treatment needs to be verified through in-depth research and clinical experiments. The new treatment method of curcumin-modified nano-gold can provide a new solution to overcome the pain points in the field of bladder cancer treatment. Summary of the Invention

[0004] The purpose of the present invention is: to solve the above technical problems, the present invention provides a method for preparing a nano-gold complex based on curcumin for the treatment of bladder cancer.

[0005] The above technical object of the present invention is achieved through the following technical solutions: Specifically, it includes the following steps:

[0006] S1: Ensure that all glassware and tools used are strictly cleaned to avoid the introduction of impurities. Dissolve chloroauric acid salt (HAuCl4) in high-purity water to prepare a solution of gold ions. Usually, the concentration is in the range of 1 mM to 10 mM.

[0007] S2: Add reducing agents, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly drip the sodium borohydride (NaBH4) solution into the gold ion solution at room temperature of 20°C - 25°C, adjust the dripping rate to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing.

[0008] S3: Control the size of the gold nanoparticles between 15 nm and 25 nm by adjusting the molar ratio of chloroaurate HAuCl4 and sodium borohydride NaBH4.

[0009] S4: During or after the synthesis of gold nanoparticles, introduce the surfactant polyvinylpyrrolidone (PVP) for modification, continuously stir the mixed solution to ensure that the surfactant fully wraps the gold nanoparticles. The reaction time may range from 5 hours to 24 hours, depending on the required particle properties.

[0010] S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, wash the particles with an appropriate solvent to remove unreacted reagents and impurities.

[0011] S6: Use techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to ensure that the shape, size, and surface properties of the gold nanoparticles meet the requirements of 15 nm - 25 nm.

[0012] S7: Dissolve curcumin in dimethyl sulfoxide (DMSO) to ensure the purity of curcumin, add the curcumin solution to the gold nanoparticle solution, and slowly stir for 10 minutes at room temperature of 20°C - 25°C to allow curcumin to interact with the gold nanoparticles.

[0013] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are as follows:

[0014] 1. Through curcumin modification, the targeting and anti-cancer effects of the complex on bladder cancer are improved.

[0015] 2. Using photothermal therapy, highly selective treatment of cancer cells is achieved, reducing damage to surrounding normal tissues. Specific Embodiments

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0017] Example 1:

[0018] A method for preparing a nano-gold complex based on curcumin for treating bladder cancer, the method comprising the following steps:

[0019] S1: Ensure that all glassware and tools used are thoroughly cleaned to avoid the introduction of impurities. Dissolve chloroaurate (HAuCl4) in high-purity water to prepare a solution of gold ions. Usually, a concentration in the range of 1 mM to 10 mM is used.

[0020] S2: Add reducing agents, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly drip the sodium borohydride (NaBH4) solution into the gold ion solution at room temperature of 20°C - 25°C, adjust the dripping rate to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing.

[0021] S3: Control the size of the gold nanoparticles to be between 15 nm and 25 nm by adjusting the molar ratio of chloroaurate HAuCl4 and sodium borohydride NaBH4.

[0022] S4: During or after the synthesis of gold nanoparticles, introduce the surfactant polyvinylpyrrolidone (PVP) for modification, continuously stir the mixed solution to ensure that the surfactant fully wraps the gold nanoparticles. The reaction time may be in the range of 5 hours to 24 hours, depending on the required particle properties.

[0023] S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, wash the particles with an appropriate solvent to remove unreacted reagents and impurities.

[0024] S6: Use techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to ensure that the shape, size, and surface properties of the gold nanoparticles meet the requirements of 15 nm - 25 nm.

[0025] S7: Dissolve curcumin in dimethyl sulfoxide (DMSO) to ensure the purity of curcumin. Add the curcumin solution to the nano-gold solution and slowly stir for 10 minutes at room temperature of 20°C - 25°C to allow curcumin to interact with the nano-gold.

[0026] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but rather to the broadest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for preparing a nano-gold complex based on curcumin for treating bladder cancer, characterized in that, The method for treating bladder cancer using curcumin-modified gold nanocomposites comprises the following steps: S1: Ensure that all glassware and tools used are rigorously cleaned to avoid the introduction of impurities. Dissolve chloroaurate (HAuCl4) in high-purity water to prepare a solution of gold ions, usually at a concentration in the range of 1 mM to 10 mM; S2: Add reducing agents, usually sodium citrate and sodium borohydride (NaBH4), to reduce gold ions to gold nanoparticles. The specific steps are to slowly add the sodium borohydride (NaBH4) solution to the gold ion solution at room temperature of 20 °C - 25 °C, adjust the dropping rate to maintain a moderate reaction rate, and stir the solution to ensure uniform mixing; S3: Control the size of the gold nanoparticles to be between 15 nm and 25 nm by adjusting the molar ratio of chloroaurate HAuCl4 and sodium borohydride NaBH4; S4: During or after the synthesis of gold nanoparticles, introduce the surfactant polyvinylpyrrolidone (PVP) for modification. Continuously stir the mixed solution to ensure that the surfactant fully coats the gold nanoparticles. The reaction time is in the range of 5 hours to 24 hours, depending on the required particle properties; S5: Use a centrifuge to separate the gold nanoparticles from the reaction mixture, and wash the particles with an appropriate solvent to remove unreacted reagents and impurities; S6: Use techniques such as transmission electron microscopy (TEM) and scanning electron microscopy (SEM) to ensure that the shape, size, and surface properties of the gold nanoparticles meet the requirements of 15 nm - 25 nm; S7: Dissolve curcumin using dimethyl sulfoxide (DMSO) to ensure the purity of curcumin. Add the curcumin solution to the gold nanoparticle solution and slowly stir at room temperature of 20 °C - 25 °C for 10 minutes to allow curcumin to interact with the gold nanoparticles.

2. The method for preparing a nano-gold complex based on curcumin for treating bladder cancer according to claim 1, wherein The reduction agent of the gold nanoparticles is used at a temperature of 22 °C.

3. A method for preparing a nanogold complex based on curcumin for treating bladder cancer according to claim 1, characterized in that, The size of the gold nanoparticles is 17 nm - 20 nm.

4. A method for preparing a nano-gold complex based on curcumin for treating bladder cancer according to claim 1, wherein, For the introduction of the surfactant polyvinylpyrrolidone (PVP) for modification, continuously stir the mixed solution, and the reaction time is controlled at 12 hours.