Multi-stage extraction apparatus for biological sample

Abstract

A multi-stage extraction device for biological specimens comprises a device body, a first magnetic rod telescopic module and a second magnetic rod telescopic module. The equipment body comprises a base, a plurality of first stirring sleeve group joints, a plurality of second stirring sleeve group joints and a driving motor; the first stirring sleeve assembly joint and the second stirring sleeve assembly joint are rotatably arranged on the base and are used for assembling a plurality of stirring sleeves; and the driving motor is used for driving the first stirring sleeve group joint and the second stirring sleeve group joint to rotate. The first magnetic rod telescopic module is used for controlling the plurality of first magnetic rods to correspondingly penetrate through the first stirring sleeve group joints respectively; and the second magnetic rod telescopic module is used for controlling a plurality of second magnetic rods to correspondingly penetrate through the second stirring sleeve group joints respectively. Wherein the first magnetic rod telescopic module and the second magnetic rod telescopic module operate independently from each other.

Description

Technical Field

[0001] The present invention relates to an extraction device, and particularly to a multi-stage biological specimen extraction device. Background Art

[0002] Generally, in a molecular biology laboratory, it is often seen that nucleic acid extraction technology is used to separate or purify nucleic acids, and then the nucleic acids are further analyzed and tested. Since nucleic acid extraction is a basic stage of many biological experiments, a large number of samples often need to be subjected to nucleic acid extraction.

[0003] In addition, in order to accelerate the efficiency of nucleic acid extraction, some researchers have developed a technology of using magnetic beads to adsorb nucleic acids. First, the cells or tissues in the specimen are dissolved to expose the nucleic acids, and then magnetic beads are added and mixed to promote the bonding of the nucleic acids with the silicon on the surface of the magnetic beads through mixing. After that, salts and impurities are washed away, and then a buffer solution is used to separate the magnetic beads from the nucleic acids. Finally, the nucleic acids can be purified and taken out.

[0004] Please refer to Figure 1 , Figure 1 which shows a three-dimensional schematic diagram of the prior art nucleic acid multi-stage extraction device used in conjunction with a kit. As Figure 1 shown, a nucleic acid multi-stage extraction device PA100 includes a bottom plate PA1, a plurality of brackets PA2 (only one is marked in the figure), a top plate PA3, eight first stirring sleeve group joints PA41 (only one is marked in the figure), eight second stirring sleeve group joints PA42 (only one is marked in the figure), a driving motor PA5, a synchronous driving frame PA6, eight first magnetic rods PA71 (only one is marked in the figure), eight second magnetic rods PA72 (only one is marked in the figure), and a telescopic driving device PA8.

[0005] As described above, the bottom plate PA1 is spaced from the top plate PA3 through a plurality of brackets PA2 to define an activity space for the synchronous driving frame PA6 to move up and down. The eight first stirring sleeve group joints PA41 and the eight second stirring sleeve group joints PA42 are arranged in two rows and rotatably disposed on the bottom plate PA1 for connecting the stirring sleeve PA200, and the driving motor 5 is further disposed on the bottom plate PA1 to drive the eight first stirring sleeve group joints PA41 and the eight second stirring sleeve group joints PA42 to rotate. The eight first magnetic rods PA71 and the eight second magnetic rods PA72 are respectively fixed at both ends of the synchronous driving frame PA6, so that when the telescopic driving device PA8 drives the synchronous driving frame PA6 to descend, the first magnetic rods PA71 fixed on the synchronous driving frame PA6 and the plurality of second magnetic rods PA72 respectively pass through the first stirring sleeve group joints PA41 and the second stirring sleeve group joints PA42 correspondingly.

[0006] In practical applications, since the number of samples that require nucleic acid extraction is often extremely large, and nucleic acid extraction may be subdivided into more steps in addition to the four basic steps of dissolution, binding, washing, and elution, in order to improve the efficiency of nucleic acid extraction, the above-mentioned multi-stage nucleic acid extraction device PA100 is usually used in conjunction with a kit PA300. The kit PA300 is provided with reagent slots PA301 in an 8×12 array (only one is marked in the figure), and thus can be divided into two groups of reagent slots PA301 in an 8×6 array for eight first stirring sleeve group connectors PA41 and eight second stirring sleeve group connectors PA42 each connected with a stirring sleeve PA200 to perform multi-stage extraction procedures PAS1 and PAS2 including six extraction steps (such as disinfection, lysis, mixing, washing, elution, and purification, etc.).

[0007] Please continue to refer to Figure 2 and Figure 3 , Figure 2 a cross-sectional schematic diagram showing the extraction steps of a prior art multi-stage nucleic acid extraction device using a set of stirring sleeves and magnetic rods; Figure 3 a cross-sectional schematic diagram showing that when a prior art multi-stage nucleic acid extraction device uses a set of stirring sleeves and magnetic rods to perform extraction steps, another set of magnetic rods is synchronously driven to extend into the kit without a stirring sleeve. As Figures 1 to 2As shown, when eight first stirring sleeve set joints PA41 and eight second stirring sleeve set joints PA42 are all assembled with stirring sleeves PA200, two sets of multi-stage extraction procedures PAS1 and PAS2 can be executed simultaneously in cooperation with the reagent kit PA300, which means that the extraction efficiency is doubled. However, when there is a multi-stage extraction procedure PAS3 that includes eight extraction steps, although the reagent kit PA300 originally preset for the simultaneous execution of two sets of multi-stage extraction procedures PAS1 and PAS2 can also be used to execute the multi-stage extraction procedure PAS3, when the stirring sleeve PA200 assembled with the first stirring sleeve set joint PA41 enters the reagent tank PA301 corresponding to the seventh extraction step according to the sequence of the multi-stage extraction procedure PAS3, the second stirring sleeve set joint PA42 will correspond to the reagent tank PA301 corresponding to the first extraction step of the multi-stage extraction procedure PAS3. And because the synchronous drive frame PA6 will drive the first magnetic rod PA71 and the second magnetic rod PA72 to pass through the first stirring sleeve set joint PA41 and the second stirring sleeve set joint PA42 respectively at the same time, when the synchronous drive frame PA6 drives the first magnetic rod PA71 to pass through the first stirring sleeve set joint PA41 and extend into the stirring sleeve PA200, the second magnetic rod PA72 will also be driven to pass through the second stirring sleeve set joint PA42. At this time, since the second stirring sleeve set joint PA42 is not assembled with the stirring sleeve PA200, the second magnetic rod PA72 directly extends into the reagent tank PA301 corresponding to the first extraction step of the multi-stage extraction procedure PAS3, resulting in mutual contamination between the second magnetic rod PA72 and the reagent tank PA301 it extends into. And when the multi-stage extraction procedure PAS3 continues, the second magnetic rod PA72 will also extend into the reagent tank PA301 of the next extraction step, further expanding the contamination. Therefore, to solve this problem, the existing method can only be designed such that when the nucleic acid multi-stage extraction device PA100 executes a program with more steps than the original default number of steps, it needs to turn and extend into the reagent tank PA301 at the other end from the other side, which is very inconvenient and will greatly reduce the extraction efficiency. Summary of the Invention

[0008] In view of the prior art, in order to improve the extraction efficiency, the existing nucleic acid multi-stage extraction equipment, in addition to arranging multiple stirring set joints in the same row, also arranges an additional row of stirring set joints to perform a large number of extraction operations at the same time. However, since the spacing between the two rows of stirring set joints is designed according to the expected extraction procedure, when the steps of the extraction procedure are increased and only one row of stirring set joints can be used to assemble the stirring sleeve to perform the extraction procedure, the synchronous drive frame will simultaneously drive the two sets of magnetic bars to extend out of the stirring rod joints, resulting in another row of magnetic bars extending into the reagent tank without the stirring sleeve to cause contamination. There is a disadvantage that the steps of the extraction procedure cannot be flexibly adjusted, and the number of extraction steps exceeding the preset cannot be performed. Therefore, the main purpose of the present invention is to provide a biological sample multi-stage extraction equipment, which can independently drive the corresponding magnetic bars through two sets of magnetic bar telescopic modules, thereby selecting to drive one set of magnetic bars to rise and fall, or selecting to drive two sets of magnetic bars to rise and fall at the same time, and thus can be flexibly changed according to the extraction procedures with different steps.

[0009] In order to solve the problems of the prior art, the present invention adopts the necessary technical means of providing a multi-stage extraction device for biological specimens, which is driven to move in multiple stages along a step direction between multiple extraction processing step positions according to an extraction step execution sequence. The multi-stage extraction device for biological specimens includes an equipment body, a first magnetic rod telescopic module and a second magnetic rod telescopic module.

[0010] The equipment body includes a base, a plurality of first stirring set joints, a plurality of second stirring set joints and at least one driving motor.

[0011] A plurality of first stirring set joints are rotatably disposed on the base and arranged in a parallel direction perpendicular to the direction in which the step is performed. A plurality of second stirring set joints are rotatably disposed on the base and arranged in the parallel direction. A driving motor is disposed on the base and is transmission-connected to the first stirring set joints and the second stirring set joints.

[0012] The first magnetic rod telescopic module includes a first magnetic rod assembly, a first telescopic driving member and a plurality of first magnetic rods. The first magnetic rod assembly is movably arranged on the device body along an insertion direction perpendicular to the step direction and the parallel direction. The first telescopic driving member is arranged on the device body to connect and drive the first magnetic rod assembly to reciprocate along the insertion direction. A plurality of first magnetic rods are arranged along the parallel direction and fixed to the first magnetic rod assembly, and when the first magnetic rod assembly is driven to move along the insertion direction, they pass through the first stirring set joints respectively and correspondingly.

[0013] The second magnetic rod telescopic module includes a second magnetic rod connector, a second telescopic driving member, and a plurality of second magnetic rods. The second magnetic rod connector is movably disposed on the device body along the insertion direction. The second telescopic driving member is disposed on the device body for connecting and driving the second magnetic rod connector to reciprocate along the insertion direction. The plurality of second magnetic rods are arranged along the parallel direction and fixed to the second magnetic rod connector, and respectively pass through the second stirring sleeve connectors correspondingly when the second magnetic rod connector is driven to move along the insertion direction. Wherein, at least one of the first stirring sleeve connectors and the second stirring sleeve connectors is used for connecting a plurality of spin tips, and the first magnetic rod telescopic module and the second magnetic rod telescopic module operate independently of each other.

[0014] In a subsidiary technical means derived from the above necessary technical means, the radius of each of the first magnetic rods is different from the radius of each of the second magnetic rods.

[0015] In a subsidiary technical means derived from the above necessary technical means, the radius of each of the first magnetic rods is the same as the radius of each of the second magnetic rods.

[0016] In a subsidiary technical means derived from the above necessary technical means, the biological sample multi-stage extraction device further includes a third magnetic rod telescopic module, and the third magnetic rod telescopic module includes a third magnetic rod connector, a third telescopic driving member, and a plurality of third magnetic rods.

[0017] The third magnetic rod connector is movably disposed on the device body along the insertion direction. The third telescopic driving member is disposed on the device body for connecting and driving the third magnetic rod connector to reciprocate along the insertion direction. The plurality of third magnetic rods are arranged along the parallel direction and fixed to the third magnetic rod connector, and respectively pass through the third stirring sleeve connectors correspondingly when the third magnetic rod connector is driven to move along the insertion direction. Wherein, the first magnetic rod telescopic module, the second magnetic rod telescopic module, and the third magnetic rod telescopic module operate independently of each other.

[0018] Preferably, the radius of at least one of each of the second magnetic rods and each of the third magnetic rods is different from the radius of each of the first magnetic rods. Another preference is that the radius of at least one of each of the second magnetic rods and each of the third magnetic rods is the same as the radius of each of the first magnetic rods.

[0019] In a subsidiary technical means derived from the above necessary technical means, the device body further includes a plurality of support frames and a top cover. The plurality of support frames respectively extend along the insertion direction and are respectively fixed to the base. The top cover is fixed to the support frames and is spaced apart from the base, and the first magnetic rod connector and the second magnetic rod connector are respectively movably arranged between the top cover and the base along the insertion direction, and the first telescopic driving member and the second telescopic driving member are respectively fixed to the top cover.

[0020] As described above, the present invention mainly selectively controls the first magnetic rod telescopic module to extend into the stirring sleeve when the stirring sleeve is connected to the first stirring sleeve connector group by the first magnetic rod telescopic module and the second magnetic rod telescopic module that operate independently of each other, and when both the first stirring sleeve connector and the second stirring sleeve connector are connected with the stirring sleeve, simultaneously controls the first magnetic rod telescopic module and the second magnetic rod telescopic module to respectively extend into the corresponding stirring sleeves, thereby effectively cooperating with the original reagent kit for more flexible operation.

[0021] Specific embodiments adopted by the present invention will be further described by the following embodiments and drawings. Description of the Drawings

[0022] Figure 1 A three-dimensional schematic diagram showing the nucleic acid multi-stage extraction device of the prior art used in combination with a reagent kit;

[0023] Figure 2 A cross-sectional schematic diagram showing the nucleic acid multi-stage extraction device of the prior art performing an extraction step with a set of stirring sleeves and magnetic rods;

[0024] Figure 3 A cross-sectional schematic diagram showing that when the nucleic acid multi-stage extraction device of the prior art performs an extraction step with a set of stirring sleeves and magnetic rods, another set of magnetic rods is synchronously driven to extend into the reagent kit without a stirring sleeve;

[0025] Figure 4 A three-dimensional schematic diagram showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention;

[0026] Figure 5 A three-dimensional schematic diagram showing three sets of reagent kits used in combination with the preferred embodiment of the present invention;

[0027] Figure 6 A three-dimensional schematic diagram showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention used in combination with the first set of reagent kits;

[0028] Figure 7 A cross-sectional schematic diagram showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention used in combination with the first set of reagent kits;

[0029] Figure 8 Schematic cross-sectional view showing that the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention controls the first magnetic rod to extend into the first stirring sleeve only through the first magnetic rod telescopic module;

[0030] Figure 9 Schematic cross-sectional view showing the cross-section of the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention at the base;

[0031] Figure 10 Schematic three-dimensional view showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the second set of reagent kits;

[0032] Figure 11 Schematic cross-sectional view showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the second set of reagent kits;

[0033] Figure 12 Schematic cross-sectional view showing that the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention controls the first magnetic rod and the second magnetic rod to extend into the stirring sleeve respectively through the first magnetic rod telescopic module and the second magnetic rod telescopic module;

[0034] Figure 13 Schematic three-dimensional view showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the third set of reagent kits; and

[0035] Figure 14 Schematic cross-sectional view showing the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the third set of reagent kits.

[0036] Wherein, reference numerals:

[0037] PA100: Nucleic acid multi-stage extraction device

[0038] PA1: Bottom plate

[0039] PA2: Bracket

[0040] PA3: Top plate

[0041] PA41: First stirring sleeve group joint

[0042] PA42: Second stirring sleeve group joint

[0043] PA5: Driving motor

[0044] PA6: Synchronous driving frame

[0045] PA71: First magnetic rod

[0046] PA72: Second magnetic rod

[0047] PA8: Telescopic drive device

[0048] PA200: Stirring sleeve

[0049] PA300: Kit

[0050] PA301: Reagent tank

[0051] PAS1, PAS2, PAS3: Multi-stage extraction procedure

[0052] 100: Biological sample multi-stage extraction equipment

[0053] 1: Equipment body

[0054] 11: Base

[0055] 111, 112: Driving gear

[0056] 113: First driven gear

[0057] 114: Second driven gear

[0058] 115: Third driven gear

[0059] 12: Support frame

[0060] 13: Top cover

[0061] 131: Connection part

[0062] 14: First stirring sleeve group joint

[0063] 15: Second stirring sleeve group joint

[0064] 16: Third stirring sleeve group joint

[0065] 17, 18: Driving motor

[0066] 171, 181: Driving shaft

[0067] 2: First magnetic rod telescopic module

[0068] 21: First magnetic rod group connector

[0069] 22: First telescopic drive part

[0070] 23: First magnetic rod

[0071] 3: Second magnetic rod telescopic module

[0072] 31: Second magnetic rod group connector

[0073] 32: Second telescopic drive part

[0074] 33: Second magnetic rod

[0075] 4: Third magnetic rod telescopic module

[0076] 41: Third magnetic rod assembly

[0077] 42: Third telescopic driving member

[0078] 43: Third magnetic rod

[0079] 200: Stirring sleeve

[0080] D1: Parallel direction

[0081] D2: Insertion direction

[0082] D3: Step execution direction

[0083] C1, C2, C3: Reagent kits

[0084] C11, C12, C13: Reagent troughs

[0085] S1, S2a, S2b, S3: Extraction step execution areas Detailed implementation manners

[0086] Please refer to Figure 4 , Figure 4 which shows a three - dimensional schematic diagram of the biological specimen multi - stage extraction device provided by the preferred embodiment of the present invention. As Figure 4 shown, a biological specimen multi - stage extraction device 100 includes a device body 1, a first magnetic rod telescopic module 2, a second magnetic rod telescopic module 3, and a third magnetic rod telescopic module 4.

[0087] The device body 1 includes a base 11, two support frames 12 (only one is marked in the figure), a top cover 13, a plurality of first stirring sleeve connectors 14 (only one is marked in the figure), a plurality of second stirring sleeve connectors 15 (only one is marked in the figure), a plurality of third stirring sleeve connectors 16 (only one is marked in the figure), and two driving motors 17 and 18.

[0088] The base 11 extends along a parallel direction D1, the two support frames 12 are respectively fixed on both sides of the base 11, and the top cover 13 is fixedly connected to the other ends of the two support frames 12 relative to the base 11, so that the base 11 and the top cover 13 are spaced apart from each other. In addition, the top cover 13 further has a connecting portion 131, and the connecting portion 131 is used to connect a robotic arm (not shown in the figure), so as to drive the entire biological specimen multi - stage extraction device 100 to move through the operation of the robotic arm.

[0089] A plurality of first stirring sleeve set joints 14 are respectively rotatably arranged on the base 11 and arranged along a juxtaposed direction D1. A plurality of second stirring sleeve set joints 15 are rotatably arranged on the base 11 and arranged along the juxtaposed direction D1. A plurality of third stirring sleeve set joints 16 are rotatably arranged on the base 11 and arranged along the juxtaposed direction D1. In this embodiment, the first stirring sleeve set joint 14 and the third stirring sleeve set joint 16 have the same radius and other dimensions.

[0090] The driving motors 17 and 18 are respectively fixedly arranged at both ends of the base 11 and are respectively drivingly connected to the first stirring sleeve set joint 14, the second stirring sleeve set joint 15 and the third stirring sleeve set joint 16.

[0091] The first magnetic rod telescopic module 2 includes a first magnetic rod connector 21, a first telescopic driving member 22 and a plurality of first magnetic rods 23 (only one is marked in the figure).

[0092] The first magnetic rod connector 21 extends along the juxtaposed direction D1 and is movably arranged between the base 11 and the top cover 13 along an inserting direction D2 perpendicular to the juxtaposed direction D1. The first telescopic driving member 22 is arranged on the top cover 13 for connecting and driving the first magnetic rod connector 21 to reciprocate along the inserting direction D2; wherein, the first telescopic driving member 22 is a telescopic cylinder with a push rod, and the first magnetic rod connector 21 is connected to the push rod, so that when the telescopic cylinder drives the push rod to expand and contract, the first magnetic rod connector 21 is driven to reciprocate in the inserting direction D2. Specifically, the above-mentioned telescopic cylinder can be a pneumatic cylinder or an electric cylinder. A plurality of first magnetic rods 23 are arranged along the juxtaposed direction D1 and are respectively fixed to the first magnetic rod connector 21, and when the first magnetic rod connector 21 is driven to move along the inserting direction D2, they respectively pass through the first stirring sleeve set joint 14.

[0093] The second magnetic rod telescopic module 3 includes a second magnetic rod connector 31, a second telescopic driving member 32 and a plurality of second magnetic rods 33 (only one is marked in the figure).

[0094] The second magnetic rod assembly 31 extends along the juxtaposed direction D1 and is movably disposed between the base 11 and the top cover 13 along the insertion direction D2. The second telescopic driving member 32 is disposed on the top cover 13 for connecting and driving the second magnetic rod assembly 31 to reciprocate along the insertion direction D2. Among them, the second telescopic driving member 32 is a telescopic cylinder with a push rod, and the second magnetic rod assembly 31 is connected to the push rod, so that when the push rod is driven to expand and contract by the telescopic cylinder, the second magnetic rod assembly 31 is driven to reciprocate in the insertion direction D2. Specifically, the above-mentioned telescopic cylinder can be a pneumatic cylinder or an electric cylinder. A plurality of second magnetic rods 33 are arranged along the juxtaposed direction D1 and fixed to the second magnetic rod assembly 31, and when the second magnetic rod assembly 31 is driven to move along the insertion direction D2, they respectively pass through the second stirring sleeve joint 15 correspondingly.

[0095] The third magnetic rod telescopic module 4 includes a third magnetic rod assembly 41, a third telescopic driving member 42, and a plurality of third magnetic rods 43 (only one is marked in the figure).

[0096] The third magnetic rod assembly 41 extends along the juxtaposed direction D1 and is movably disposed between the base 11 and the top cover 13 along the insertion direction D2. The third telescopic driving member 42 is disposed on the top cover 13 for connecting and driving the third magnetic rod assembly 41 to reciprocate along the insertion direction D2. Among them, the third telescopic driving member 42 is a telescopic cylinder with a push rod, and the second magnetic rod assembly 41 is connected to the push rod, so that when the push rod is driven to expand and contract by the telescopic cylinder, the second magnetic rod assembly 31 is driven to reciprocate in the insertion direction D2. Specifically, the above-mentioned telescopic cylinder can be a pneumatic cylinder or an electric cylinder. A plurality of third magnetic rods 43 are arranged along the juxtaposed direction D1 and fixed to the third magnetic rod assembly 41, and when the third magnetic rod assembly 41 is driven to move along the insertion direction D2, they respectively pass through the third stirring sleeve joint 16 correspondingly.

[0097] As described above, the first magnetic rod telescopic module 2, the second magnetic rod telescopic module 3, and the third magnetic rod telescopic module 4 operate independently of each other. In this embodiment, the radius of the first magnetic rod 23 is smaller than the radius of the second magnetic rod 33, and the radius of the third magnetic rod 43 is the same as the radius of the first magnetic rod 23, but this is not limited in other embodiments.

[0098] In other embodiments, the biological specimen multi-stage extraction device 100 may also only have the combination of the first magnetic rod telescopic module 2 cooperating with the first stirring sleeve group joint 14 and the second magnetic rod telescopic module 3 cooperating with the second stirring sleeve group joint 15, without the combination of the third magnetic rod telescopic module 4 cooperating with the third stirring sleeve group joint 16. In addition, the biological specimen multi-stage extraction device 100 may also only have the combination of the first magnetic rod telescopic module 2 cooperating with the first stirring sleeve group joint 14 and the third magnetic rod telescopic module 4 cooperating with the third stirring sleeve group joint 16, without the combination of the second magnetic rod telescopic module 3 cooperating with the second stirring sleeve group joint 15. At this time, the original third magnetic rod telescopic module 4 and the third stirring sleeve group joint 16 can be regarded as another second magnetic rod telescopic module and another second stirring sleeve group joint.

[0099] Please continue to refer to Figure 5 , Figure 5 the three-dimensional schematic diagram showing the three sets of reagent kits used in the preferred embodiment of the present invention. As Figure 4 and Figure 5 shown, the biological specimen multi-stage extraction device 100 of this embodiment can be used in cooperation with three reagent kits C1 (only one is marked in the figure), or with three reagent kits C2 (only one is marked in the figure), or with three reagent kits C3 (only one is marked in the figure).

[0100] Among them, the reagent kits C1 and C2 have the same specifications. The reagent kit C1 contains 96 reagent slots C11 arranged in an 8×12 array, and the reagent kit C2 also contains 96 reagent slots C21 arranged in an 8×12 array. Moreover, the length, width, height and other dimensions of the reagent slot C11 and the reagent slot C12 are the same, which means that the reagent kits C1 and C2 can be regarded as the same structure. However, the main difference between the two is that the reagent kit C1 is used to cooperate with the above-mentioned biological specimen multi-stage extraction device 100 to perform an extraction procedure within an extraction step execution area S1, while the reagent kit C2 is used to cooperate with the above-mentioned biological specimen multi-stage extraction device 100 to perform an extraction procedure within two extraction step execution areas S2a and S2b simultaneously.

[0101] More specifically, when the biological sample multi-stage extraction device 100 executes the extraction step in the extraction step execution area S1, it is driven according to an extraction step execution sequence to move multi-stagedly between a plurality of extraction treatment step positions (corresponding to eight reagent tanks C11 arranged in sequence along the step progress direction D3 in the extraction step execution area S1) along a step progress direction D3. When the biological sample multi-stage extraction device 100 performs the extraction procedure in the extraction step execution areas S2a and S2b, it is driven according to another extraction step execution sequence to move multi-stagedly between a plurality of extraction treatment step positions (corresponding to six reagent tanks C21 arranged in sequence along the step progress direction D3 in the extraction step execution area S2a and six reagent tanks C21 arranged in sequence along the step progress direction D3 in the extraction step execution area S2b) along the step progress direction D3.

[0102] In addition, the reagent kit C3 includes 24 reagent tanks C31 arranged in a 4×6 array, and the length, width, and height of the reagent tank C31 are all twice that of the reagent tank C11 or the reagent tank C12. Among them, the reagent kit C3 is used to cooperate with the above-mentioned biological sample multi-stage extraction device 100 to perform the extraction procedure in an extraction step execution area S3. When the biological sample multi-stage extraction device 100 executes the extraction step execution area S3, it is driven according to another extraction step execution sequence to move multi-stagedly between a plurality of extraction treatment step positions (corresponding to six reagent tanks C31 arranged in sequence along the step progress direction D3 in the extraction step execution area S3) along the step progress direction D3.

[0103] In practical applications, the above-mentioned reagent kits C1, C2, and C3 are, for example, filled with reagents, biological samples (DNA or RNA), buffer solutions, or cleaning solutions in the reagent tanks C11, C21, and C31 respectively corresponding to the extraction step execution areas S1, S2a, S2b, and S3.

[0104] Please continue to refer to Figures 6 to 8 , Figure 6 a three-dimensional schematic diagram showing the biological sample multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the first set of reagent kits; Figure 7 a cross-sectional schematic diagram showing the biological sample multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the first set of reagent kits; Figure 8 a cross-sectional schematic diagram showing that the biological sample multi-stage extraction device provided by the preferred embodiment of the present invention only controls the first magnetic rod to extend into the first stirring sleeve through the first magnetic rod telescopic module.

[0105] As Figures 4 to 8As shown, in this application example, since the extraction step execution area S1 preset in the kit C1 contains eight steps, compared with the 96 reagent slots C11 arranged in an 8×12 array in the kit C1, the array of reagent slots C11 required for each of the eight steps corresponding to the extraction step execution area S1 of each kit C1 is 8×8. Therefore, each kit C1 can only be used by the biological sample multi-stage extraction device 100 to perform the extraction procedure within one extraction step execution area S1. Thus, in this example, the user only needs to respectively sleeve a plurality of stirring sleeves 200 (only one is marked in the figure) on the first stirring sleeve group joint 14 (or it can also be implemented by only sleeving on the second stirring sleeve group joint 16), and then drive the stirring sleeve 200 to alternately extend into the reagent slot C11 corresponding to each extraction treatment step position along the step progress direction D3 according to the corresponding extraction step execution sequence by controlling the biological sample multi-stage extraction device 100 within the extraction step execution area S1. And when the stirring sleeve 200 enters the reagent slot C11, the first telescopic driving member 22 will drive the first magnetic rod group joint 21 to drive the first magnetic rod 23 to extend into the stirring sleeve 200 connected to the first stirring sleeve group joint 14 along the insertion direction D2, so that the first magnetic rod 23 adsorbs and fixes the magnetic beads in the reagent slot C11 on the outer surface of the stirring sleeve 200. At this time, by driving the first stirring sleeve group joint 14 to rotate through the driving motors 17 and 18, the mixing of the magnetic beads with the reagent or biological sample in the reagent slot C11 can be effectively promoted.

[0106] As described above, in this application example, the biological sample multi-stage extraction device 100 only drives the first magnetic rod group joint 21 to drive the first magnetic rod 23 to move along the insertion direction D2 through the first telescopic driving member 22, and does not drive the second magnetic rod group joint 31 to drive the second magnetic rod 33 to move along the insertion direction D2 through the second telescopic driving member 32, nor does it drive the third magnetic rod group joint 41 to drive the third magnetic rod 43 to move along the insertion direction D2 through the third telescopic driving member 42. Therefore, the second magnetic rod 33 will not extend into the reagent slot C11 when there is no stirring sleeve 200 sleeved on the second stirring sleeve group joint 15. Similarly, the third magnetic rod 43 will not extend into the reagent slot C11 when there is no stirring sleeve 200 sleeved on the third stirring sleeve group joint 16, thereby effectively preventing the second magnetic rod 33 or the third magnetic rod 33 from being contaminated with the reagent or biological sample in the reagent slot C11.

[0107] Please continue to refer to Figure 9 , Figure 9 which shows a schematic cross-sectional view of the biological sample multi-stage extraction device provided by the preferred embodiment of the present invention taken at the base. As Figures 4 to 9As shown, in this embodiment, two driving gears 111 and 112, multiple first driven gears 113 (only one is labeled in the figure), multiple second driven gears 114 (only one is labeled in the figure), and multiple third driven gears 115 (only one is labeled in the figure) are further provided inside the base 11; among them, the driving gear 111 is sleeved and fixed on a driving shaft 171 of the driving motor 17, so as to be driven by the driving motor 17 to rotate, and the driving gear 112 is sleeved and fixed on a driving shaft 181 of the driving motor 18, so as to be driven by the driving motor 18 to rotate.

[0108] Multiple first driven gears 113 are respectively rotatably sleeved on multiple first magnetic rods 23, multiple second driven gears 114 are respectively rotatably sleeved on multiple second magnetic rods 33, and multiple third driven gears 115 are respectively rotatably sleeved on multiple third magnetic rods 43; among them, since the multi-stage extraction device 100 for biological specimens in this embodiment uses two driving motors 17 and 18 to synchronously drive the rotation of multiple first driven gears 113, multiple second driven gears 114, and multiple third driven gears 115, in order to evenly disperse the burden of the driving motors 17 and 18, mainly multiple first driven gears 113, multiple second driven gears 114, and multiple third driven gears 115 are divided into three groups, and the driving motor 17 is drivingly connected to one group of first driven gears 113 (eight in each group meshing with each other), two groups of second driven gears 114 (four in each group meshing with each other), and one group of third driven gears 115 (eight in each group meshing with each other), and the driving motor 18 is drivingly connected to the other two groups of first driven gears 113, the other one group of second driven gears 114, and the other two groups of third driven gears 115.

[0109] However, in other embodiments, when the configured numbers of the first driven gear 113, the second driven gear 114, and the third driven gear 115 are small (for example, eight, four, and eight respectively), only one driving motor 17 can be used to output power.

[0110] Please continue to refer to Figures 10 to 12 , Figure 10 a three-dimensional schematic diagram showing the multi-stage extraction device for biological specimens provided by the preferred embodiment of the present invention in cooperation with the second set of reagent kits; Figure 11 a cross-sectional schematic diagram showing the multi-stage extraction device for biological specimens provided by the preferred embodiment of the present invention in cooperation with the second set of reagent kits; Figure 12 a cross-sectional schematic diagram showing the multi-stage extraction device for biological specimens provided by the preferred embodiment of the present invention controlling the first magnetic rod and the second magnetic rod to respectively extend into the stirring sleeve through the first magnetic rod telescopic module and the second magnetic rod telescopic module.

[0111] As shown in Figures 10 to 12As shown, when the biological specimen multi-stage extraction device 100 is used to cooperate with the kit C2 to synchronously perform the second extraction step in the execution areas S2a and S2b, multiple stirring sleeves 200 can be respectively sleeved on the first stirring sleeve group joint 14 and the third stirring sleeve group joint 16. When the biological specimen multi-stage extraction device 100 simultaneously performs the extraction process in the extraction step execution areas S2a and S2b, the first telescopic driving member 22 and the third telescopic driving member 42 respectively drive the first magnetic rod group joint 21 and the third magnetic rod group joint to drive the first magnetic rod 23 and the third magnetic rod 43 to respectively extend into the stirring sleeves 200 assembled by the first stirring sleeve group joint 14 and the second stirring sleeve group joint 16 along the insertion direction D2.

[0112] Please continue to refer to Figure 13 and Figure 14 , Figure 13 showing a three-dimensional schematic diagram of the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the third group of kits; Figure 14 showing a cross-sectional schematic diagram of the biological specimen multi-stage extraction device provided by the preferred embodiment of the present invention in cooperation with the third group of kits.

[0113] As Figure 13 and Figure 14 shown, when the biological specimen multi-stage extraction device 100 is used to cooperate with the kit C3 to perform an extraction step in the execution area S3, multiple stirring sleeves 200 can be respectively sleeved on the second stirring sleeve group joint 15. When the biological specimen multi-stage extraction device 100 performs the extraction process in the extraction step execution area S3, the second telescopic driving member 32 drives the second magnetic rod group joint 31 to drive the second magnetic rod 33 to respectively extend into the stirring sleeves 200 assembled by the second stirring sleeve group joint 15 along the insertion direction D2.

[0114] In summary, the biological specimen multi-stage extraction device of the present invention can, through the first magnetic rod telescopic module and the second magnetic rod telescopic module that operate independently of each other, selectively control the first magnetic rod telescopic module to extend into the stirring sleeve when the first stirring sleeve group joint is assembled with a stirring sleeve, and when both the first stirring sleeve group joint and the second stirring sleeve group joint are assembled with stirring sleeves, simultaneously control the first magnetic rod telescopic module and the second magnetic rod telescopic module to respectively extend into the corresponding stirring sleeves, thereby effectively cooperating with the original kit to perform more flexible extraction operations.

[0115] Through the detailed description of the above preferred specific embodiments, it is hoped that the features and spirit of the present invention can be more clearly described, rather than limiting the scope of the present invention by the above-disclosed preferred specific embodiments. On the contrary, the purpose is to hope to cover various changes and equivalent arrangements within the scope of the patent application of the present invention.

Claims

1. A biological sample multi-stage extraction device is driven to move multi-stage between a plurality of extraction processing step positions along a step progress direction according to an execution sequence of an extraction step. The biological sample multi-stage extraction device includes: A device body, including: A base; A plurality of first stirring sleeve group joints rotatably arranged on the base and arranged in a side-by-side direction perpendicular to the step progress direction; A plurality of second stirring sleeve group joints rotatably arranged on the base and arranged in the side-by-side direction; and At least one driving motor arranged on the base and drivingly connected to the first stirring sleeve group joints and the second stirring sleeve group joints to drive the first stirring sleeve group joints and the second stirring sleeve group joints to rotate; A first magnetic rod telescopic module, including: A first magnetic rod connecting member movably arranged on the device body along an insertion direction perpendicular to the step progress direction and the side-by-side direction; A first telescopic driving member arranged on the device body for connecting and driving the first magnetic rod connecting member to reciprocate along the insertion direction; and A plurality of first magnetic rods arranged in the side-by-side direction and fixed to the first magnetic rod connecting member, and respectively passing through the first stirring sleeve group joints when the first magnetic rod connecting member is driven to move along the insertion direction; and A second magnetic rod telescopic module, including: A second magnetic rod connecting member movably arranged on the device body along the insertion direction; A second telescopic driving member arranged on the device body for connecting and driving the second magnetic rod connecting member to reciprocate along the insertion direction; and A plurality of second magnetic rods arranged in the side-by-side direction and fixed to the second magnetic rod connecting member, and respectively passing through the second stirring sleeve group joints when the second magnetic rod connecting member is driven to move along the insertion direction; Among them, At least one of the first stirring sleeve group joints and the second stirring sleeve group joints is used for connecting a plurality of spin tips, and the first magnetic rod telescopic module and the second magnetic rod telescopic module operate independently of each other.

2. The multi-stage extraction device for biological specimens according to claim 1, wherein, The radius of each of the first magnetic rods is different from the radius of each of the second magnetic rods.

3. The multi-stage extraction device for biological specimens according to claim 1, wherein, The radius of each of the first magnetic rods is the same as the radius of each of the second magnetic rods.

4. The biological sample multi-stage extraction device according to claim 1, further including: A third magnetic rod telescopic module, including: A third magnetic rod connecting member movably arranged on the device body along the insertion direction; A third telescopic driving member arranged on the device body for connecting and driving the third magnetic rod connecting member to reciprocate along the insertion direction; and A plurality of third magnetic rods arranged in the side-by-side direction and fixed to the third magnetic rod connecting member, and respectively passing through the third stirring sleeve group joints when the third magnetic rod connecting member is driven to move along the insertion direction; Among them, The first magnetic rod telescopic module, the second magnetic rod telescopic module and the third magnetic rod telescopic module operate independently of each other.

5. The biological specimen multi-stage extraction device according to claim 4, wherein, The radius of at least one of each of the second magnetic rods and each of the third magnetic rods is different from the radius of each of the first magnetic rods.

6. The biological specimen multi-stage extraction device according to claim 4, wherein, The radius of at least one of each of the second magnetic rods and each of the third magnetic rods is the same as the radius of each of the first magnetic rods.

7. The biological sample multi-stage extraction device according to claim 1, wherein, The device body further includes: a plurality of support frames respectively extending along the insertion direction and respectively fixed to the base; and a top cover fixed to the support frames and spaced apart from the base, and the first magnetic rod connector and the second magnetic rod connector are respectively movably disposed between the top cover and the base along the insertion direction, and the first telescopic driving member and the second telescopic driving member are respectively fixed to the top cover.

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