Stirring rod for extracting biological sample

Abstract

The invention relates to a stirring rod for extracting a biological sample. The stirring rod sequentially comprises an assembling part, a body part and a tail end part along an extending direction, the assembling part is used for being connected to a rotating assembling head of a biological sample extraction mechanism in a sleeved mode. The body part is formed by integrally extending from the assembling part along the extending direction, and is provided with a peripheral surface and at least one first convex rib structure convexly extending from the peripheral surface; and the tail end part is integrally connected to the body part, is provided with a conical surface which is adjacent to the body part and gradually contracts along the extension direction, is provided with an end surface far away from the body part, and comprises at least one tail end stirring structure which convexly extends from the end surface along the extension direction.

Description

Technical Field

[0001] The present invention relates to a stirring rod, and more particularly to a stirring rod for extracting biological specimens. Background Art

[0002] In a general chemical laboratory, various experiments are often carried out by means of mixing or separation. Among them, a common mixing method is to use the rotation of a stirring rod to achieve rapid stirring and mixing. Especially in a molecular biology laboratory, in order to effectively extract nucleic acids, the existing technology mainly uses the rotation of a stirring rod to stir magnetic beads and specimens, so as to promote the bonding of nucleic acids with silicon on the surface of the magnetic beads. Then, salts and impurities are washed away, and then a buffer solution is used to separate the magnetic beads and nucleic acids. Finally, the nucleic acids can be purified and taken out.

[0003] Please refer to Figure 1 and Figure 2 , Figure 1 which shows a three-dimensional schematic diagram of a stirring rod of the prior art used in conjunction with a biological specimen extraction mechanism and a reagent kit; Figure 2 which shows a three-dimensional schematic diagram of a stirring rod of the prior art. As Figure 1 and Figure 2 shown, a stirring rod PA100 includes a sleeve portion PA1, a tube body PA2, and an end face PA3.

[0004] The sleeve portion PA1 is used for sleeving and fixing to a connector (not shown in the figure) of a biological specimen extraction mechanism PA200. The tube body PA2 extends from the sleeve portion PA1 to the end face PA3, and three rib structures PA21 (only one is marked in the figure) protrude from the peripheral surface of the tube body PA2 evenly distributed.

[0005] Please continue to refer to Figure 3 , Figure 3 which shows a plan view of the stirring rod of the prior art extending into a reagent tube with a high liquid volume. As Figures 1 to 3 shown, when the sleeve portion PA1 is sleeved and fixed to the connector of the biological specimen extraction mechanism PA200, it can be used in conjunction with a reagent kit PA300. The reagent kit PA300 includes a reagent kit body PA301 and a plurality of test tubes PA302 (only one is shown in the figure), and the test tubes PA302 are placed in a receiving groove (not marked in the figure) of the reagent kit body PA301. Thus, after the tube body PA2 of the stirring rod PA100 is driven by the biological specimen extraction mechanism PA200 to extend into the test tube PA302, the stirring rod PA100 can be driven to rotate, so that the rib structure PA21 drives the liquid PA400 filled in the test tube PA302 to flow, and drives the magnetic beads PA401 mixed in the liquid PA400 to stir.

[0006] Please continue to refer to Figure 4 ,Figure 4 A schematic plan view showing a stirring rod of the prior art extending into a reagent tube with a low liquid volume. As Figure 3 shown in Figure 4 , when the liquid PA400a filled in the test tube PA302 is of low liquid volume, although the stirring rod PA100 can also extend into the test tube PA302 and rotate, since the length of the rib structure PA21 often does not extend to the end face PA3, for the liquid PA400a with too low liquid volume, the rib structure PA21 cannot fully play its due stirring and mixing role, nor can it drive the magnetic beads PA401a to stir. Only the part of the end face PA3 and the sleeve body PA2 extending into the liquid PA400a can drive the liquid PA400a to rotate and flow, and thus there is a problem of poor end disturbance effect.

[0007] Please continue to refer to Figure 5 , Figure 5 A schematic plan view showing the phenomenon of residual liquid droplets occurring when the stirring rod of the prior art extends out of the reagent tube. As Figures 3 to 5 shown, whether the stirring rod PA100 extends into a test tube PA302 with a high liquid volume or a low liquid volume, when the stirring rod PA100 leaves the test tube PA302, there will be some liquid (not marked in the figure) remaining on the end face PA3, and thus it will drip everywhere during the process of moving the stirring rod PA100, causing contamination. SUMMARY OF THE INVENTION

[0008] In view of the fact that in the prior art, although the existing stirring sleeve is provided with a rib structure to drive the liquid to flow, since the bottom of the test tube is usually an arc surface with a gradually decreasing diameter, and the presence of the rib structure will increase the maximum radius of the stirring rod, in order to avoid the rib structure from colliding with the gradually shrinking bottom of the test tube, the axial length of the rib structure is usually limited. Therefore, when the liquid volume in the test tube is too low, the rib structure is very likely unable to extend into the liquid to disturb the liquid, resulting in a problem of poor end disturbance; thus, the main object of the present invention is to provide a stirring rod for extracting biological specimens, which can solve the problem of poor end disturbance effect.

[0009] In order to solve the problems of the prior art, the necessary technical means adopted by the present invention is to provide a stirring rod for extracting biological specimens, which sequentially includes a set of connecting parts, a body part, and a terminal part along an extending direction.

[0010] The set of connecting parts is used to connect to a rotary joint of a biological specimen extraction mechanism. The body part is integrally formed and extended from the set of connecting parts along the extending direction, and has an outer peripheral surface and at least one first rib structure protruding from the outer peripheral surface. The terminal part is integrally connected to the body part, has a conical surface adjacent to the body part and gradually shrinking along the extending direction, and has an end face away from the body part, and includes at least one terminal stirring structure protruding from the end face along the extending direction.

[0011] In a secondary technical means derived from the above necessary technical means, the end portion further includes at least one second rib structure, and the at least one second rib structure protrudes integrally from the conical surface. Preferably, the at least one second rib structure is arranged alternately with the at least one first rib structure and the at least one end agitating structure.

[0012] In a secondary technical means derived from the above necessary technical means, the end portion further includes a tapered section and an end extension section. The tapered section extends integrally from the body portion along the extension direction and tapers toward the extension direction to form the conical surface. The end extension section extends integrally from the other end of the tapered section relative to the body portion along the extension direction and has the end face.

[0013] Preferably, the end portion further includes at least one second rib structure, and the at least one second rib structure protrudes integrally from the conical surface and extends to the end extension section. In addition, the at least one second rib structure is arranged alternately with the at least one first rib structure and the at least one end agitating structure.

[0014] In a secondary technical means derived from the above necessary technical means, the area of the end face is smaller than the cross-sectional area of the body portion.

[0015] As described above, the present invention mainly has an end agitating structure provided on the end face of the end portion, so that the stirring rod can effectively agitate the liquid with a low liquid volume, thereby achieving the purpose of stirring and mixing.

[0016] Specific embodiments adopted by the present invention will be further described by the following embodiments and drawings. Description of the Drawings

[0017] Figure 1 A three-dimensional schematic diagram showing a stirring rod of the prior art used in cooperation with a biological specimen extraction mechanism and a reagent kit;

[0018] Figure 2 A three-dimensional schematic diagram showing a stirring rod of the prior art;

[0019] Figure 3 A plan view showing a stirring rod of the prior art extending into a reagent tube with a high liquid volume;

[0020] Figure 4 A plan view showing a stirring rod of the prior art extending into a reagent tube with a low liquid volume;

[0021] Figure 5 A plan view showing a stirring rod of the prior art extending out of the reagent tube with a residual liquid drop phenomenon;

[0022] Figure 6Stereoscopic schematic diagram showing the stirring rod for extracting biological specimens of the present invention;

[0023] Figure 7 Planar schematic diagram showing the stirring rod for extracting biological specimens of the present invention extending into a reagent tube with a high liquid volume;

[0024] Figure 8 Planar schematic diagram showing the stirring rod for extracting biological specimens of the present invention extending into a reagent tube with a low liquid volume; and

[0025] Figure 9 Planar schematic diagram showing the stirring rod for extracting biological specimens of the present invention extending out of the reagent tube.

[0026] Among them, reference numerals:

[0027] PA100: Stirring rod

[0028] PA1: Sleeve part

[0029] PA2: Casing body

[0030] PA21: Rib structure

[0031] PA3: End face

[0032] PA200: Biological specimen extraction mechanism

[0033] PA300: Kit

[0034] PA301: Kit body

[0035] PA302: Test tube

[0036] PA400, PA400a: Liquid

[0037] PA401, PA401a: Magnetic beads

[0038] 100: Stirring rod for extracting biological specimens

[0039] 1: Connecting part

[0040] 2: Body part

[0041] 21: Outer peripheral surface

[0042] 22, 23: First rib structure

[0043] 3: End part

[0044] 31: Tapered section

[0045] 311: Conical surface

[0046] 32: End extension section

[0047] 321: End face

[0048] 33, 34: Second rib structure

[0049] 35, 36: End agitation structure

[0050] 200: Test tube

[0051] 201: Liquid

[0052] 202: Magnetic bead

[0053] D1: Extension direction Detailed implementation manner

[0054] Please refer to Figure 6 , Figure 6 which is a three-dimensional schematic diagram showing the stirring rod for extracting biological specimens of the present invention. As Figure 6 shown, a stirring rod 100 for extracting biological specimens sequentially includes a set of connecting parts 1, a body part 2, and an end part 3 along an extension direction D1.

[0055] The set of connecting parts 1 is used to connect to a rotating joint of a biological specimen extraction mechanism (not shown in the figure, equivalent to the biological specimen extraction mechanism PA200 of the prior art).

[0056] The body part 2 is integrally formed and extended from the set of connecting parts 1 along the extension direction D1, and has an outer peripheral surface 21 and two first rib structures 22 and 23 protruding from the outer peripheral surface 21, and the two first rib structures 22 and 23 also extend along the extension direction D1 respectively.

[0057] The end part 3 includes a tapered section 31, an end extension section 32, two second rib structures 33 and 34, and two end agitation structures 35 and 36.

[0058] The tapered section 31 is integrally formed and extended from the body part 2 along the extension direction D1, and tapers towards the extension direction D1 to form a conical surface 311.

[0059] The end extension section 32 is integrally formed and extended from the other end of the tapered section 31 relative to the body part 2 along the extension direction D1, and has an end face 321, and the area of the end face 321 is smaller than the cross-sectional area of the body part 2.

[0060] The two second rib structures 33 and 34 respectively protrude integrally from opposite sides of the conical surface 311 and extend to the end extension section 32. Among them, the two second rib structures 33 and 34 of the present embodiment respectively protrude radially. And since in the present embodiment, the extension direction D1 is an axial direction, the two second rib structures 33 and 34 are respectively perpendicular to the extension direction D1. In addition, the two second rib structures 33 and 34 are respectively arranged in an alternating pattern with the two first rib structures 22 and 23.

[0061] The two end agitating structures 35 and 36 respectively protrude from the end face 321 along the extension direction D1 and are located on opposite sides of the end face 321. And in the present embodiment, the two end agitating structures 35 and 36 are further arranged in an alternating pattern with the two second rib structures 33 and 34.

[0062] In practical applications, the stirring rod 100 for extracting biological specimens of the present embodiment is a hollow sleeve structure. After being fixedly sleeved on the rotating joint of the biological specimen extraction mechanism through the connecting portion 1, the biological specimen extraction mechanism can insert a magnetic rod into the stirring rod 100 for extracting biological specimens. As described above, although in the present embodiment, the main body portion 2 includes the two first rib structures 22 and 23, and the end portion 3 includes the two second rib structures 33 and 34 and the two end agitating structures 35 and 36, in other embodiments, each may also include one; in addition, although the first rib structures 22 and 23, the second rib structures 33 and 34, and the end agitating structures 35 and 36 all extend along the extension direction D1 in the present embodiment, in other embodiments, they may also be helically extended or obliquely extended, and all can achieve the effect of disturbing the flow.

[0063] Please continue to refer to Figure 7 , Figure 7 a schematic plan view showing the stirring rod for extracting biological specimens of the present invention extending into a reagent tube with a high liquid volume. As Figure 6 and Figure 7 shown, when the stirring rod 100 for extracting biological specimens of the present invention is inserted into a test tube 200 and the two first rib structures 22 and 23 respectively extend into the liquid 201 filled in the test tube 200, driven by the biological specimen extraction mechanism connected to the connecting portion 1, the two first rib structures 22 and 23 will agitate the liquid 201 in the test tube 200 to form a vortex, and make the magnetic beads 202 filled in the liquid 201 move along with the vortex, thereby enabling the liquid 201 and the magnetic beads 202 to be fully mixed.

[0064] Among them, since the second convex rib structures 33 and 34 are further provided on the tapered section 31 and the end extension section 32 of the end portion 3, and the two end stirring structures 35 and 36 are provided on the end face 321, the liquid 201 at the bottom of the test tube 200 will also generate corresponding disturbances and vortices. In addition, since the two second convex rib structures 33 and 34 are arranged alternately with the two first convex rib structures 22 and 23 and the two end stirring structures 35 and 36 respectively, the efficiency of disturbing the liquid 201 can be improved by the staggered arrangement.

[0065] Please continue to refer to Figure 8 , Figure 8 Fig. shows a schematic plan view of the stirring rod for extracting biological specimens of the present invention extending into a reagent tube with a low liquid volume. As Figure 6 and Figure 8 shown, when the liquid 201 filled in the test tube 200 into which the stirring rod 100 for extracting biological specimens of the present invention is inserted is of low liquid volume, although the two first convex rib structures 22 and 23 cannot extend into the liquid 201, the two second convex rib structures 33 and 34 and the two end stirring structures 35 and 36 of the end portion 3 will extend into the low-liquid-volume liquid 201. Thereby, driven by the biological specimen extraction mechanism, the two second convex rib structures 33 and 34 and the two end stirring structures 35 and 36 will also stir the liquid 201 in the test tube 200 to form a vortex, and the magnetic beads 202 filled in the liquid 201 will move along with the vortex, thereby achieving the effect of fully mixing the liquid 201 and the magnetic beads 202.

[0066] Please continue to refer to Figure 9 , Figure 9 Fig. shows a schematic plan view of the stirring rod for extracting biological specimens of the present invention extending out of the reagent tube. As Figure 9 shown, since the tapered section 31 of the stirring rod 100 for extracting biological specimens of the present invention tapers from the body portion 2 along the extension direction D1 to the end extension section 32, and the end extension section 32 continues to extend along the extension direction D1 to form the end face 321, the area of the end face 321 can be effectively reduced. Thereby, when the stirring rod 100 for extracting biological specimens of the present invention extends out of the test tube 200, the liquid (not shown) in the test tube 200 is not likely to remain on the end face 321, thereby effectively avoiding the problem of liquid drop residue.

[0067] In summary, compared with the prior art stirring sleeve having the problem of poor end disturbance, the stirring rod for extracting biological specimens of the present invention is provided with an end stirring structure on the end face of the end portion, so as to disturb the liquid in the low-liquid-volume use environment through the end stirring structure, and can effectively achieve the effect of stirring and mixing. In addition, since the area of the end face of the end portion of the stirring rod for extracting biological specimens of the present invention is reduced, it can more effectively prevent the liquid from remaining on the end face and generating liquid drops.

[0068] Through the detailed description of the above preferred specific embodiments, it is hoped that the features and spirit of the present invention can be more clearly described, rather than limiting the scope of the present invention by the above-disclosed preferred specific embodiments. On the contrary, the purpose is to hope to cover various changes and equivalent arrangements within the scope of the patent application of the present invention.

Claims

1. A stirring rod for extracting biological specimens, sequentially including along an extending direction: A connecting portion for connecting to a rotating connector of a biological specimen extraction mechanism; A body portion integrally formed and extending from the connecting portion along the extending direction, having an outer peripheral surface and at least one first rib structure protruding from the outer peripheral surface; and A terminal portion integrally connected to the body portion, having a conical surface adjacent to the body portion and tapering along the extending direction, and having an end surface away from the body portion, and including at least one terminal stirring structure protruding from the end surface along the extending direction.

2. The stirring rod for extracting a biological specimen according to claim 1, wherein, The terminal portion further includes at least one second rib structure integrally protruding from the conical surface.

3. The stirring rod for extracting a biological specimen according to claim 2, wherein, The at least one second rib structure is arranged in an alternating pattern with the at least one first rib structure and the at least one terminal stirring structure respectively.

4. The stirring rod for extracting a biological specimen according to claim 1, wherein, The terminal portion further includes: A tapered section integrally extending from the body portion along the extending direction and tapering along the extending direction to form the conical surface; and A terminal extension section integrally extending from the other end of the tapered section relative to the body portion along the extending direction and having the end surface.

5. The stirring rod for extracting a biological specimen as claimed in claim 4, wherein, The terminal portion further includes at least one second rib structure integrally protruding from the conical surface and extending to the terminal extension section.

6. The stirring rod for extracting a biological specimen according to claim 5, wherein, The at least one second rib structure is arranged in an alternating pattern with the at least one first rib structure and the at least one terminal stirring structure respectively.

7. The stirring rod for extracting a biological specimen as described in claim 1, wherein, The area of the end surface is smaller than the cross-sectional area of the body portion.

Images