Fermentation nutrition preparation device and preparation method thereof

Abstract

The invention discloses a fermentation nutrition preparation device and a preparation method thereof, the fermentation nutrition preparation device comprises foot pads, a fermentation box main body, a nitrogen replacement unit, a box cover, a transparent plate, an air pipe and a bacterium preparation mechanism, and the core of the device is the bacterium preparation mechanism; through cooperation of the independent normal saline bin, the activator bin and the three inoculation structures, time-sharing accurate activation and directional feeding of lactic acid bacteria, saccharomycetes and bacillus are achieved. The bacterium preparation mechanism integrates spiral lifting, bacterium bin overturning and anti-caking design, it is ensured that bacterium powder and normal saline are redissolved according to a set proportion and then are accurately fed, and timing adding of a cysteine activator is synchronously completed. The preparation method is based on the device, the flora symbiotic metabolism path is optimized through gradient regulation and control of temperature, dissolved oxygen and stirring rate parameters, and ultrasonic-assisted enzymolysis and low-temperature membrane separation technologies are used in parallel. According to the method, the problems of strain competitive inhibition, artificial pollution, low product purity and the like are solved, and an efficient and controllable gradient fermentation system is formed.

Description

Technical Field

[0001] The present invention relates to the technical field of fermentation preparation, and specifically relates to a fermentation nutrition preparation device and a preparation method thereof. Background Art

[0002] As an important raw material in the fields of functional foods and medicine, the production of fermentation nutritional preparations depends on multi-strain co-fermentation technology; in traditional processes, symbiotic systems of lactic acid bacteria, yeasts, and bacillus subtilis can synthesize active ingredients such as antibacterial peptides and vitamins.

[0003] Currently, Chinese Patent Application No.: CN201921851888.4 discloses a fermentation device for bacillus subtilis microbial preparations. The fermentation device includes a main fermentation chamber, a material cracking chamber, a material saccharification chamber, a strain culture chamber, a bacterial liquid separation chamber, a nutrient solution preparation chamber, an oxygen supply device, a spray drying chamber, and an intelligent digital control device; a terahertz generator, a first braking baffle, and a first humidification device are arranged in the material cracking chamber. The material cracking chamber is communicated with the material saccharification chamber. A color sensor, a third ultraviolet sterilization device, and a second braking baffle are arranged in the strain culture chamber. The strain culture chamber is communicated with the bacterial liquid separation chamber. The main fermentation chamber is communicated with the material saccharification, nutrient solution preparation chamber, bacterial liquid separation chamber, and the oxygen supply device. The main fermentation chamber is connected to the spray drying chamber through a pipeline.

[0004] However, in the existing fermentation nutritional preparation production technology, it is usually single-strain fermentation. For multi-strain fermentation, centralized strain warehouses are used to synchronously activate and put in strains, resulting in conflicts in the metabolic timing sequence of strains and competitive inhibition, and significant loss of active ingredients in the products; in addition, it is not easy to achieve dynamic coordination of activation, temperature control, and stirring, resulting in restrictions on the symbiotic efficiency of the flora and affecting the product yield and quality. Summary of the Invention

[0005] The purpose of the present invention is to provide a fermentation nutrition preparation device and a preparation method thereof to solve the problems raised in the above background art.

[0006] To achieve the above object, the present invention adopts the following technical solutions: A fermentation nutrient preparation device, including a footrest, a fermentation tank main body, a nitrogen replacement unit, a tank cover, a transparent plate, a trachea, and a bacteria distribution mechanism. The fermentation tank main body is fixedly connected to the top side of the footrest. The nitrogen replacement unit is disposed through the upper part of the right side wall of the fermentation tank main body, and the tank cover is installed in the middle of the top of the fermentation tank main body. The transparent plate is embedded in the middle of the tank cover. The trachea is disposed through the left rear side of the top of the fermentation tank main body. A bacteria distribution mechanism for performing three-gradient inoculation is disposed in the upper rear part of the fermentation tank main body. The bacteria distribution mechanism includes a physiological saline tank fastened to the upper rear part of the fermentation tank main body, a first inoculation structure, a second inoculation structure, and a third inoculation structure fastened to the bottom side of the physiological saline tank from left to right in sequence, an activator tank fixedly connected to the right side of the physiological saline tank, three first liquid outlet pipes installed at the bottom front part of the physiological saline tank, and a second liquid outlet pipe fastened to the bottom front part of the activator tank. The first inoculation structure, the second inoculation structure, and the third inoculation structure are respectively used for loading lactic acid bacteria, yeast, and bacillus. Three flow pumps are disposed in the lower front part of the physiological saline tank, and the front liquid outlet ends of the three flow pumps are respectively connected to the three first liquid outlet pipes. The three first liquid outlet pipes are respectively disposed above the first inoculation structure, the second inoculation structure, and the third inoculation structure. One flow pump is disposed in the activator tank, and the front liquid outlet end of the flow pump is connected to the second liquid outlet pipe.

[0007] Preferably, a stirring main body, a heating module, and a monitoring module are disposed inside the fermentation tank main body. The stirring main body is perpendicular to the middle side inside the fermentation tank main body. The heating module is installed on the inner wall of the fermentation tank main body. The monitoring module includes a temperature sensor, a pH sensor, and a dissolved oxygen sensor.

[0008] Preferably, the first inoculation structure, the second inoculation structure, and the third inoculation structure have the same structure and size and are located at the same horizontal height position. A triangular support block is disposed at the front bottom part of the physiological saline tank, and the rear side of the support block is fixed to the first inoculation structure, the second inoculation structure, and the third inoculation structure.

[0009] Preferably, the first inoculation structure includes a rectangular seat fastened to the top side of the physiological saline tank, a chute opened in the middle of the front part of the rectangular seat, a partition fixed above the chute, a first motor disposed above the partition, a screw rod connected to the bottom output end of the first motor, an internally threaded moving block threadedly connected to the outer surface of the screw rod, and an inversion component fastened to the front side of the internally threaded moving block. The rear side of the first motor is fastened to the rectangular seat. The top side of the screw rod penetrates and rotates through the middle of the partition, and the bottom end of the screw rod is rotatably connected to the rectangular seat. The rear side of the internally threaded moving block is longitudinally slidably connected to the rectangular seat, and the front part of the internally threaded moving block penetrates and is disposed inside the chute. The left and right sides of the rear side of the inversion component are in sliding contact with the rectangular seat.

[0010] Preferably, the inversion component includes a frame with its rear middle side fastened to the internally threaded moving block. A second motor is fastened to the middle side inside the frame. The front output end of the second motor is connected to a rotating frame, which includes a rotating shaft column and a rectangular support piece integrally formed on the top side of the front part of the rotating shaft column. The rotating shaft column penetrates and rotates through the middle part of the front side of the frame, and the rear end of the rotating shaft column is connected to the front output end of the second motor. A third motor is fastened to the top side of the rectangular support piece. The bottom output end of the third motor is connected to a driving gear, and the front side of the driving gear is connected to the bacteria placing component, and the bottom of the rear side of the bacteria placing component is fixed to the rotating shaft column.

[0011] Preferably, the bacteria placing component includes a bracket fixed to the rotating shaft column at the rear side, a gear ring block penetrating and rotating through the inner side of the front part of the bracket, a silo fixedly connected to the inner side of the gear ring block, a carrier plate rotatably connected to the bottom side of the silo, and a fixed stirring rod penetrating and rotating through the middle and lower side inside the silo. The middle side of the bottom of the driving gear is rotatably connected to the bracket, and the front side of the driving gear meshes with the gear ring block. The rear side of the carrier plate is fixed to the bracket, and the bottom of the fixed stirring rod is fixed to the middle side of the carrier plate.

[0012] Preferably, the front side of the bracket is arc-shaped, and arc-shaped grooves are provided on both the upper and lower sides inside the arc-shaped part. Protrusions are provided in the middle of both the upper and lower sides of the gear ring block, and the protrusions on both the upper and lower sides are respectively slidably connected inside the two arc-shaped grooves.

[0013] In addition, the present invention also provides a preparation method of a fermentation nutrient preparation, which adopts the above-mentioned fermentation nutrient preparation device, and includes the following steps: S1. Raw material pretreatment: Mix soybean meal and corn starch according to a mass ratio of 2.5:1 to 3.5:1, and pass through a 80-mesh sieve after two-stage treatment of coarse crushing and ultrafine crushing; S2. Enzymatic hydrolysis treatment: Add 0.5%-1.2% compound protease to the raw materials, and perform ultrasonic-assisted treatment for 1.5-2.5 hours under the conditions of 40°C-50°C and pH 6.0-7.0, and the ultrasonic frequency is 18-22 kHz; S3. Gradient fermentation: Use the fermentation nutrient preparation device to inoculate lactic acid bacteria, yeast and bacillus three times, and redissolve with 0.85% normal saline 30 minutes before inoculation. The adjacent inoculation intervals of the three inoculations are 7-9 hours, and the stirring rate is dynamically adjusted throughout the process to 200-800 rpm and the temperature to 32°C-40°C; S4. Product extraction: After the fermentation broth is filtered through a 0.1-0.2 μm ceramic membrane, an ultrafiltration membrane with a cut-off molecular weight of 8-12 kDa is used to separate the active ingredients; S5. Drying treatment: After ultrafiltration membrane separation, vacuum freeze-drying treatment is adopted, and the freeze-drying conditions are pre-freezing temperature of -45°C to -35°C, sublimation stage pressure of 10-30 Pa, and analysis stage temperature of 25°C-30°C.

[0014] Preferably, the gradient fermentation specifically includes: a) When inoculating lactic acid bacteria for the first time, control the temperature at 32°C ± 1°C, the stirring rate at 180 - 220 rpm, and the dissolved oxygen content at 5 - 6 mg / L; b) Before inoculating yeast for the second time, add 0.08% - 0.12% cysteine, raise the temperature to 38°C - 40°C, increase the stirring rate to 450 - 550 rpm, and the dissolved oxygen content to 8 - 10 mg / L; c) When inoculating bacillus for the third time, lower the temperature to 34°C - 36°C, adjust the stirring rate to 280 - 320 rpm, and the dissolved oxygen content to 3 - 4 mg / L.

[0015] Preferably, the ratio of the inoculum amounts of the lactic acid bacteria, yeast, and bacillus for the three inoculations is (1 - 1.5):(2 - 2.5):(0.8 - 1.2), and the pH value fluctuation is detected not to exceed ±1 before each inoculation.

[0016] Compared with the prior art, the beneficial effects of the present invention are: The fermentation nutrition preparation device of the present invention optimizes the use of a bacteria - matching mechanism. Through independently controlled normal saline tanks and activator tanks, it realizes the time - sharing and precise activation and dosing of lactic acid bacteria, yeast, and bacillus. The modular design of the three inoculation structures, combined with the flow pump control system, ensures that the normal saline re - dissolution ratios of different strains are consistent, and the cysteine activator can be added directionally and regularly, significantly reducing the competitive inhibition of strains. The synergistic effect of the spiral lifting and tilting components solves the problem of uneven dispersion caused by bacterial powder caking and simultaneously avoids the pollution risk introduced by manual operation. Through the integration of stage - by - stage activation, directional dosing of strains, and automated processes, an efficient and controllable gradient inoculation system is formed.

[0017] The fermentation nutrition preparation method of the present invention organically combines the activation of strains, timing regulation, and post - treatment processes. By adjusting gradient parameters, it optimizes the symbiotic efficiency of the microbial community, and combines ultrasonic - assisted enzymatic hydrolysis and low - temperature membrane separation technologies to improve the product purity while completely retaining the active ingredients, significantly enhancing the product yield and quality. Brief Description of the Drawings

[0018] Figure 1 It is a schematic structural diagram of the fermentation nutrition preparation device of the present invention; Figure 2 It is a schematic structural diagram of the bacteria - matching mechanism of the present invention; Figure 3 It is a schematic structural diagram of the first inoculation structure of the present invention; Figure 4 It is a schematic structural diagram of the connection between the internal - thread moving block and the tilting component of the present invention; Figure 5 It is a schematic structural diagram of the tilting component of the present invention; Figure 6 For the present invention Figure 5 is a schematic diagram of the upward view structure; Figure 7 is a flowchart of the preparation method of the fermentation nutritional preparation of the present invention.

[0019] In the figure: foot pad - 1, fermentation tank main body - 2, nitrogen replacement unit - 3, tank cover - 4, transparent plate - 5, air pipe - 6, bacteria - matching mechanism - 7, physiological saline tank - 71, first inoculation structure - 72, second inoculation structure - 73, third inoculation structure - 74, activator tank - 75, first liquid outlet pipe - 76, second liquid outlet pipe - 77, rectangular seat - 721, sliding groove - 722, partition - 723, first motor - 724, screw rod - 725, internally threaded moving block - 726, inversion assembly - 727, frame - 7271, second motor - 7272, rotating frame - 7273, third motor - 7274, moving gear - 7275, bacteria - placing component - 7276, support - 72761, gear ring block - 72762, silo - 72763, carrier plate - 72764, fixed stirring rod - 72765. Specific embodiments

[0020] In order to further explain the technical solution of the present invention, the following will be elaborated in detail through specific embodiments.

[0021] Please refer to Figure 1 and Figure 2 , the present invention provides a fermentation nutritional preparation device, including a foot pad 1, a fermentation tank main body 2, a nitrogen replacement unit 3, a tank cover 4, a transparent plate 5, an air pipe 6 and a bacteria - matching mechanism 7. The top side of the foot pad 1 is fixedly connected to the fermentation tank main body 2. The nitrogen replacement unit 3 is penetrated through the upper part of the right side wall of the fermentation tank main body 2, and the tank cover 4 is installed in the middle of the top of the fermentation tank main body 2. An anaerobic environment can be established before fermentation through the nitrogen replacement unit 3 to reduce contamination by miscellaneous bacteria. The transparent plate 5 is embedded in the middle of the inside of the tank cover 4 to achieve non - contact observation and avoid temperature control fluctuations caused by frequent opening of the tank cover. The air pipe 6 is penetrated through the left rear side of the top of the fermentation tank main body 2. The bacteria - matching mechanism 7 for performing three - stage gradient inoculation is arranged at the upper rear part inside the fermentation tank main body 2, which can achieve three - stage gradient inoculation of lactic acid bacteria, yeast and bacillus in stages, improve the survival rate of the strains, reduce the competitive inhibition generated during the symbiosis of multiple strains, and improve the product yield. Moreover, a stirring main body, a heating module and a monitoring module are arranged inside the fermentation tank main body 2. The stirring main body is perpendicular to the middle side inside the fermentation tank main body 2. The heating module is installed on the inner wall of the fermentation tank main body 2. The monitoring module includes a temperature sensor, a pH sensor and a dissolved oxygen sensor, which can dynamically adjust the heating temperature control and can adjust the stirring rate through the feedback of the dissolved oxygen.

[0022] Among them, the bacteria inoculation mechanism 7 includes a physiological saline tank 71 fastened to the upper rear inside of the fermentation tank main body 2, a first inoculation structure 72, a second inoculation structure 73, and a third inoculation structure 74 fastened to the bottom side of the physiological saline tank 71 in sequence from left to right, an activator tank 75 fixedly connected to the right side of the physiological saline tank 71, three first liquid outlet pipes 76 installed at the front bottom side of the physiological saline tank 71, and a second liquid outlet pipe 77 fastened to the front bottom side of the activator tank 75. The physiological saline tank 71 is internally filled with 0.85% physiological saline. The first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74 are respectively used to load lactic acid bacteria, yeast, and bacillus. The activator tank 75 is used to load cysteine as a metabolic activator. Three flow pumps are arranged at the front lower side inside the physiological saline tank 71, and the front liquid outlet ends of the three flow pumps are respectively connected to the three first liquid outlet pipes 76. The three first liquid outlet pipes 76 are respectively arranged above the first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74. By adding 0.85% physiological saline into the first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74 respectively, the lactic acid bacteria, yeast, and bacillus are activated, and different strains can be put in at different times. One flow pump is arranged inside the activator tank 75, and the front liquid outlet end of this flow pump is connected to the second liquid outlet pipe 77 to discharge cysteine into the fermentation broth to activate the metabolic activity of yeast. The structures and sizes of the first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74 are the same and are located at the same horizontal height position. A triangular support block is arranged at the front bottom of the physiological saline tank 71, and the rear side of the support block is fixed to the first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74 to enhance the structural stability.

[0023] Please refer to Figures 2 - 6, the present invention provides a fermentation nutrient preparation device. The first inoculation structure 72 includes a rectangular seat 721 whose top side is fastened to the physiological saline tank 71, a chute 722 opened in the middle of the front part of the rectangular seat 721, a partition 723 fixed above the chute 722, a first motor 724 arranged above the partition 723, a screw rod 725 connected to the bottom output end of the first motor 724, an internally threaded moving block 726 threadedly connected to the outer surface of the screw rod 725, and an inoculation component 727 fastened to the front side of the internally threaded moving block 726. The rear side of the first motor 724 is fastened to the rectangular seat 721. Using the first motor 724 as a power source to drive the screw rod 725 to rotate, under the rotation of the screw rod 725, the internally threaded moving block 726 drives the inoculation component 727 to adjust the position in the longitudinal direction, so that the inoculation component 727 can be moved close to the upper part of the fermentation broth, reducing the problem of liquid splashing when pouring in the strains. The top side of the screw rod 725 penetrates and rotates through the middle of the partition 723, and the bottom end of the screw rod 725 is rotatably connected to the rectangular seat 721 to ensure the stability of the rotation of the screw rod 725. The rear side of the internally threaded moving block 726 is longitudinally slidably connected to the rectangular seat 721, and the front part of the internally threaded moving block 726 penetrates and is arranged inside the chute 722. The left and right sides of the rear side of the inoculation component 727 are in sliding contact with the rectangular seat 721 to ensure the stable effect of the internally threaded moving block 726 driving the inoculation component 727 to perform a displacement action.

[0024] Among them, the inoculation component 727 includes a frame 7271 whose middle part of the rear side is fastened to the internally threaded moving block 726. A second motor 7272 is fastened in the middle of the inside of the frame 7271. The front output end of the second motor 7272 is connected to a rotating frame 7273. The rotating frame 7273 includes a rotating shaft column and a rectangular support piece integrally formed on the top side of the front part of the rotating shaft column. The rotating shaft column penetrates and rotates through the middle of the front side of the frame 7271, and the rear end of the rotating shaft column is connected to the front output end of the second motor 7272. A third motor 7274 is fastened to the top side of the rectangular support piece. The bottom output end of the third motor 7274 is connected to a moving gear 7275. The front side of the moving gear 7275 is connected to an inoculation part 7276, and the bottom of the rear side of the inoculation part 7276 is fixed to the rotating shaft column. By driving the rotating frame 7273 to perform a rotating and flipping action through the second motor 7272, the third motor 7274, the moving gear 7275 and the inoculation part 7276 can perform a flipping action synchronously, facilitating pouring the strains into the fermentation broth. And the third motor 7274 drives the inoculation part 7276 to act through the moving gear 7275 to break up the agglomerated strain powder inside the inoculation part 7276, improving the dispersion uniformity.

[0025] Among them, the bacteria placing component 7276 includes a bracket 72761 fixedly connected to the rear side of the rotating shaft column, a gear ring block 72762 rotatably penetrating through the inner front part of the bracket 72761, a silo 72763 fixedly connected to the inner side of the gear ring block 72762, a carrier plate 72764 rotatably connected to the bottom side of the silo 72763, and a fixed stirring rod 72765 rotatably penetrating through the middle and lower part inside the silo 72763. The middle side of the bottom of the moving gear 7275 is rotatably connected to the bracket 72761, and the front side of the moving gear 7275 is meshed with the gear ring block 72762. Under the rotation of the moving gear 7275, the gear ring block 72762 is rotated under the support of the bracket 72761 to generate a position shift, so that the silo 72763 rotates synchronously with the gear ring block 72762. The rear side of the carrier plate 72764 is fixed to the bracket 72761, and the bottom of the fixed stirring rod 72765 is fixed to the middle side of the carrier plate 72764. When the silo 72763 rotates above the carrier plate 72764, the fixed stirring rod 72765 remains in place inside the silo 72763, thereby crushing the caked bacterial species powder inside. The front side of the bracket 72761 is arc-shaped, and arc-shaped grooves are provided on both the upper and lower sides inside the arc-shaped part. Protrusions are provided in the middle of both the upper and lower sides of the gear ring block 72762, and the protrusions on both the upper and lower sides are respectively slidably connected to the inside of the two arc-shaped grooves, playing a role of supporting and limiting, and ensuring a stable sliding action of the gear ring block 72762 inside the bracket 72761.

[0026] The working principle of the fermentation nutrient preparation device is as follows: First, place the fermentation broth inside the fermentation tank main body 2, then open the tank cover 4, pour 0.85% normal saline into the normal saline tank 71, pour cysteine into the activator tank 75, and then place the lactic acid bacteria powder, yeast powder, and bacillus powder into the silos 72763 of the first inoculation structure 72, the second inoculation structure 73, and the third inoculation structure 74 respectively; Second, during the fermentation process of the fermentation broth, when inoculating lactic acid bacteria for the first time, control the temperature at 32°C ± 1°C, the stirring rate at 180 - 220 rpm, and the dissolved oxygen content at 5 - 6 mg / L. And 30 minutes in advance, control the first liquid discharge pipe 76 above the first inoculation structure 72 to discharge 0.85% normal saline into the silo 72763 of the first inoculation structure 72, so that the lactic acid bacteria powder is mixed with 0.85% normal saline. Then control the third motor 7274 to start, and the third motor 7274 drives the moving gear 7275 to rotate. Under the rotation of the moving gear 7275, the gear ring block 72762 rotates under the support of the bracket 72761 to generate a transposition, so that the silo 72763 rotates synchronously with the gear ring block 72762. When the silo 72763 rotates above the carrier plate 72764, the fixed stirring rod 72765 remains in place inside the silo 72763, thereby crushing the agglomerated strain powder inside. Then, 30 minutes later, control the first motor 724 to start to rotate the screw 725. Under the rotation of the screw 725, the internal thread moving block 726 drives the pouring component 727 to move downward to approach above the fermentation broth. Then control the second motor 7272 to drive the rotating frame 7273 to perform a rotating and flipping action, so that the third motor 7274, the moving gear 7275, and the bacteria placing component 7276 perform flipping actions synchronously, and pour the normal saline with lactic acid bacteria in the silo 72763 into the fermentation broth for fermentation treatment, reducing the problem of liquid splashing when pouring the strain; Third, during the fermentation process of the fermentation broth, when inoculating yeast for the second time, add 0.08% - 0.12% cysteine into the fermentation broth through the second liquid discharge pipe 77 in the activator bin 75 before inoculating yeast, and raise the temperature to 38°C - 40°C, increase the stirring rate to 450 - 550 rpm, and the dissolved oxygen content to 8 - 10 mg / L. And 30 minutes in advance, control the first liquid discharge pipe 76 above the second inoculation structure 73 to discharge 0.85% normal saline into the yeast powder, so that the yeast powder is mixed with 0.85% normal saline and the agglomerated strain powder inside is crushed. After 30 minutes, pour the normal saline with yeast into the fermentation broth for fermentation treatment; Fourth, during the fermentation process of the fermentation broth, when inoculating bacillus for the third time, cool the fermentation broth to 34°C - 36°C, adjust the stirring rate to 280 - 320 rpm, and the dissolved oxygen content to 3 - 4 mg / L. And 30 minutes in advance, control the first liquid discharge pipe 76 above the third inoculation structure 74 to discharge 0.85% normal saline into the bacillus powder, so that the bacillus powder is mixed with 0.85% normal saline and the agglomerated strain powder inside is crushed. After 30 minutes, pour the normal saline with yeast into the fermentation broth for fermentation treatment to complete the preparation of the fermentation broth.

[0027] Please refer to Figures 1 - 7 , the present invention provides a preparation method of a fermentation nutrient preparation, which adopts the above-mentioned fermentation nutrient preparation device, including the following steps: S1. Raw material pretreatment: Mix soybean meal and corn starch in a mass ratio of 3:1. After coarse grinding with a particle size ≤ 2 mm, perform ultrafine grinding with a particle size ≤ 80 μm, and pass through an 80-mesh sieve. S2. Enzymatic hydrolysis treatment: Add 0.8% compound protease (papain: flavor protease = 1:1) to the raw materials. Under the conditions of 45 °C and pH 6.5, apply ultrasonic treatment at 20 kHz for 2 hours. S3. Gradient fermentation: Use a fermentation nutrient preparation device: First inoculation: Inject 30 L of enzymatic hydrolysate into the fermenter, and start nitrogen replacement (oxygen content < 0.5%). Parameter setting: Temperature 32 °C, stirring rate 200 rpm, dissolved oxygen content 5.5 mg / L. Put 1.2 g of lactic acid bacteria powder, dissolve it in 200 mL of normal saline, activate it for 30 minutes, and then automatically feed it. Second inoculation (with an 8-hour interval): Add 0.1% cysteine, raise the temperature to 39 °C, increase the stirring rate to 500 rpm, and the dissolved oxygen content to 9 mg / L. Put 2.2 g of yeast powder, dissolve it in 200 mL of normal saline. Third inoculation (with another 8-hour interval): Lower the temperature to 35 °C, the stirring rate is 300 rpm, and the dissolved oxygen content is 3.5 mg / L. Put 1.0 g of bacillus powder, dissolve it in 200 mL of normal saline. S4. Product extraction: After the fermentation broth is filtered through a 0.15-μm ceramic membrane, it is further separated by a 10-kDa ultrafiltration membrane, and the membrane flux is maintained at 55 L / (m 2 ·h) for 6 hours. S5. Drying treatment: The filtrate is pre-frozen at -40 °C and then freeze-dried under a pressure of 20 Pa. The final analysis temperature is 28 °C to obtain a light yellow powder.

[0028] The above are only the preferred examples of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A fermentation nutrient preparation device, comprising a footrest (1), a fermentation tank main body (2) is fixedly connected to the top side of the footrest (1), a nitrogen replacement unit (3) is arranged through the upper part of the right side wall of the fermentation tank main body (2), and a tank cover (4) is installed in the middle of the top of the fermentation tank main body (2). A transparent plate (5) is embedded in the middle of the inner part of the tank cover (4). A trachea (6) is arranged through the left rear side of the top of the fermentation tank main body (2). It is characterized in that: It further includes a bacteria preparation mechanism (7) arranged at the upper rear inside the fermentation tank main body (2) for three-stage gradient inoculation. The bacteria preparation mechanism (7) includes a physiological saline tank (71) fastened to the upper rear inside the fermentation tank main body (2), a first inoculation structure (72), a second inoculation structure (73), and a third inoculation structure (74) fastened to the bottom side of the physiological saline tank (71) in sequence from left to right, an activator tank (75) fixedly connected to the right side of the physiological saline tank (71), three first liquid outlet pipes (76) installed at the front bottom side of the physiological saline tank (71), and a second liquid outlet pipe (77) fastened to the front bottom side of the activator tank (75). The first inoculation structure (72), the second inoculation structure (73), and the third inoculation structure (74) are respectively used for loading lactic acid bacteria, yeast, and bacillus. Three flow pumps are arranged at the front lower side inside the physiological saline tank (71), and the front liquid outlet ends of the three flow pumps are respectively connected to the three first liquid outlet pipes (76). The three first liquid outlet pipes (76) are respectively arranged above the first inoculation structure (72), the second inoculation structure (73), and the third inoculation structure (74). One flow pump is arranged inside the activator tank (75), and the front liquid outlet end of this flow pump is connected to the second liquid outlet pipe (77).

2. The fermented nutritional preparation device according to claim 1, characterized in that: A stirring main body, a heating module, and a monitoring module are arranged inside the fermentation tank main body (2). The stirring main body is vertical in the middle side inside the fermentation tank main body (2), the heating module is installed on the inner wall of the fermentation tank main body (2), and the monitoring module includes a temperature sensor, a pH sensor, and a dissolved oxygen sensor.

3. The fermented nutritional preparation device according to claim 1, characterized in that: The first inoculation structure (72), the second inoculation structure (73), and the third inoculation structure (74) have the same structure and size and are located at the same horizontal height position. A triangular support block is arranged at the front bottom of the physiological saline tank (71), and the rear side of the support block is fixed to the first inoculation structure (72), the second inoculation structure (73), and the third inoculation structure (74).

4. The fermentation nutrient preparation device according to claim 1, wherein: The first inoculation structure (72) includes a rectangular seat (721) with its top side fastened to the physiological saline tank (71), a chute (722) opened at the middle front of the rectangular seat (721), a partition plate (723) fixed above the chute (722), a first motor (724) arranged above the partition plate (723), a screw rod (725) connected to the bottom output end of the first motor (724), an internally threaded moving block (726) threadedly connected to the outer surface of the screw rod (725), and an inversion assembly (727) fastened to the front side of the internally threaded moving block (726). The rear side of the first motor (724) is fastened to the rectangular seat (721). The top side of the screw rod (725) penetrates and rotates through the middle of the partition plate (723), and the bottom end of the screw rod (725) is rotatably connected to the rectangular seat (721). The rear side of the internally threaded moving block (726) is longitudinally slidably connected to the rectangular seat (721), and the front part of the internally threaded moving block (726) penetrates and is arranged inside the chute (722). The left and right sides of the rear side of the inversion assembly (727) are in sliding contact with the rectangular seat (721).

5. The fermented nutritional preparation device according to claim 4, characterized in that: The placement and inversion assembly (727) includes a frame (7271) whose rear middle side is fastened to the internally threaded moving block (726). A second motor (7272) is fastened to the middle side inside the frame (7271). The front output end of the second motor (7272) is connected to a rotating frame (7273). The rotating frame (7273) includes a rotating shaft column and a rectangular support piece integrally formed on the top side of the front part of the rotating shaft column. The rotating shaft column penetrates and rotates through the middle part of the front side of the frame (7271), and the rear end of the rotating shaft column is connected to the front output end of the second motor (7272). A third motor (7274) is fastened to the top side of the rectangular support piece. The bottom output end of the third motor (7274) is connected to a moving gear (7275). The front side of the moving gear (7275) is connected to the bacteria placement component (7276), and the bottom of the rear side of the bacteria placement component (7276) is fixed to the rotating shaft column.

6. The fermented nutritional preparation device according to claim 5, wherein: The bacteria placement component (7276) includes a bracket (72761) whose rear side is fixed to the rotating shaft column, a gear ring block (72762) that penetrates and rotates through the inner side of the front part of the bracket (72761), a silo (72763) fixedly connected to the inner side of the gear ring block (72762), a carrier plate (72764) rotatably connected to the bottom side of the silo (72763), and a fixed stirring rod (72765) that penetrates and rotates through the middle and lower side inside the silo (72763). The middle side of the bottom of the moving gear (7275) is rotatably connected to the bracket (72761), and the front side of the moving gear (7275) meshes with the gear ring block (72762). The rear side of the carrier plate (72764) is fixed to the bracket (72761), and the bottom of the fixed stirring rod (72765) is fixed to the middle side of the carrier plate (72764).

7. The fermented nutritional preparation device according to claim 6, wherein: The front side of the bracket (72761) is arc-shaped, and arc-shaped grooves are provided on both the upper and lower sides inside the arc-shaped part. Protrusions are provided in the middle of both the upper and lower sides of the gear ring block (72762), and the protrusions on both the upper and lower sides are respectively slidably connected to the inside of the two arc-shaped grooves.

8. A preparation method of a fermented nutritional preparation, characterized in that, Using the fermentation nutrient preparation device according to any one of claims 1-7, the following steps are included: S1. Raw material pretreatment: Mix soybean meal and corn starch in a mass ratio of 2.5:1 to 3.5:1, and pass through an 80-mesh sieve after two-stage treatment of coarse crushing and ultrafine crushing. S2. Enzymolysis treatment: Add 0.5%-1.2% compound protease to the raw materials, and perform ultrasonic-assisted treatment for 1.5-2.5 hours under the conditions of 40°C-50°C and pH 6.0-7.

0. The ultrasonic frequency is 18-22 kHz. S3. Gradient fermentation: Use the fermentation nutrient preparation device to inoculate lactic acid bacteria, yeast, and bacillus in three batches, and redissolve them with 0.85% physiological saline 30 minutes before inoculation. The adjacent inoculation intervals for the three inoculations are 7-9 hours, and the stirring rate is dynamically adjusted throughout the process to 200-800 rpm and the temperature to 32°C-40°C. S4. Product extraction: After the fermentation broth is filtered through a 0.1-0.2 μm ceramic membrane, an ultrafiltration membrane with a molecular weight cut-off of 8-12 kDa is used to separate the active ingredients. S5. Drying treatment: After ultrafiltration membrane separation, vacuum freeze-drying treatment is adopted. The freeze-drying conditions are a pre-freezing temperature of -45°C to -35°C, a pressure of 10 - 30 Pa in the sublimation stage, and a temperature of 25°C - 30°C in the desorption stage.

9. The preparation method of a fermentation nutrient preparation according to claim 8, wherein, The gradient fermentation specifically includes: a). When inoculating lactic acid bacteria for the first time, control the temperature at 32°C ± 1°C, the stirring rate at 180 - 220 rpm, and the dissolved oxygen content at 5 - 6 mg / L; b). Before inoculating yeast for the second time, add 0.08% - 0.12% cysteine, raise the temperature to 38°C - 40°C, increase the stirring rate to 450 - 550 rpm, and the dissolved oxygen content to 8 - 10 mg / L; c). When inoculating bacillus for the third time, lower the temperature to 34°C - 36°C, adjust the stirring rate to 280 - 320 rpm, and the dissolved oxygen content to 3 - 4 mg / L.

10. The preparation method of a fermented nutritional preparation according to claim 8, characterized in that, The bacterial quantity ratio of the lactic acid bacteria, yeast, and bacillus inoculated three times is (1 - 1.5):(2 - 2.5):(0.8 - 1.2), and the pH value fluctuation is detected to be no more than ±1 before each inoculation.

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