Fungus culture device for skin infection diagnosis

Abstract

The invention relates to the technical field of fungus diagnosis, in particular to a fungus culture device for skin infection diagnosis. A mother culture dish and a son culture dish are embedded in the culture box; and a medicine bottle and a liquid storage tube are fixedly mounted in the incubator. A fixed sleeve is fixedly mounted in the mounting frame, and a dropping pipette is fixedly mounted in the fixed sleeve; a plurality of groups of guide pipes are slidably mounted in the culture box, and collecting bags are fixedly mounted on the guide pipes; a collecting structure, a transferring structure and a liquid supplementing structure are arranged on the incubator; the transfer structure can drive the guide pipe to move in the vertical direction and the radial direction of the fixing table. When the catheter moves, the collection structure extrudes the collection bag to collect a fungus sample; the liquid supplementing structure can increase the flow speed of liquid medicine in the medicine bottle to the mother culture dish so as to supplement sample loss; the transfer structure, the collection structure and the liquid injection structure are matched with one another, so that fungus samples can be automatically transferred to the sub-culture dishes containing different inhibition drugs under the condition that the internal environment of the culture box is not changed.

Description

Technical Field

[0001] The present invention relates to the technical field of fungal diagnosis, and specifically to a fungal culture device for skin infection diagnosis. Background Art

[0002] Common types of skin infections include bacteria, fungi, viruses, and parasites. The common diseases corresponding to each type, for example, bacterial infections may include impetigo and cellulitis; fungal infections include athlete's foot and tinea cruris; viral infections include herpes and warts; parasitic infections include scabies and lice; among which fungal infections are particularly common.

[0003] Making a correct judgment on the fungal species before treating skin fungal infections can contribute to the correct and effective conduct of treatment. Commonly used methods include Wood's lamp examination (such as for tinea capitis) and skin scraping microscopy; when the skin scraping microscopy shows a negative result, but fungal infection is highly suspected, or it is necessary to identify the fungal species (such as stubborn infections and onychomycosis), fungal culture can be used to judge the fungal species.

[0004] Skin fungal culture is an important laboratory method for diagnosing fungal infections. By isolating and culturing pathogenic fungi, the infecting fungal species can be identified and treatment can be guided. Commonly, an incubator is used for culturing, that is, the skin fungal sample is placed in a pre-prepared culture dish, and the culture dish is placed in the incubator for culturing; the incubator can provide suitable humidity, temperature and other conditions for the reproduction of fungi; after a period of time, the originally hardly noticeable fungi will form visible colonies in the culture dish, thus facilitating the diagnosis of the fungal species. However, due to the use of antibiotics, fungi have developed certain drug resistance, which means that after diagnosing the fungal species of skin infections, drug resistance diagnosis is still required to facilitate medical staff to make the best treatment plan.

[0005] Common drug resistance diagnosis is to collect the fungi in the colony into multiple culture dishes containing different inhibitory drugs for drug resistance culture, and judge the drug resistance status of the fungi by observing the survival performance of the fungi in the culture dishes. A collection stick is commonly used for collection.

[0006] When using a collection stick to collect and culture the fungi in different culture dishes, the culture dish needs to be taken out of the incubator, and the collection stick is used to scrape the colony to collect the fungi, and then the collection stick is put into the culture dish containing the inhibitory drug. To achieve rapid diagnosis, drug resistance diagnosis of the fungi with multiple drugs is often required. At this time, when using a collection stick for collection, the collection conditions of each sample will be different (collection environment, collection time point, and the extrusion force of the collection stick on the colony), and these differences may cause accidental situations during the drug resistance diagnosis of the fungi, affecting the correct diagnosis of the drug resistance of the fungi. Summary of the Invention

[0007] The purpose of the present invention is to provide a fungal culture device for skin infection diagnosis, so as to solve the problems raised in the above-mentioned background technology.

[0008] To achieve the above purpose, the present invention provides the following technical solutions: A fungal culture device for skin infection diagnosis, including an incubator; a fixed table and an installation frame are fixedly installed inside the incubator, and a rotating table is rotatably installed on the fixed table; a mother culture dish and multiple groups of sub-culture dishes are fitted on the fixed table and the rotating table; A medicine bottle and a liquid storage pipe are fixedly installed inside the incubator; a liquid inlet hole is opened on the liquid storage pipe; and the medicine bottle and the liquid storage pipe are communicated through the liquid inlet hole; a fixed sleeve is fixedly installed inside the installation frame, and a drip pipe communicated with the liquid storage pipe is fixedly installed inside the fixed sleeve; the end of the drip pipe is located directly above the mother culture dish; Multiple groups of conduits are slidably installed inside the incubator, a collection tube is clamped at one end of the conduit, and a collection sac is fixedly installed at the other end; A collection structure, a transfer structure and a liquid replenishment structure that cooperate with each other are arranged on the incubator; The transfer structure can drive the conduit to move vertically and radially along the fixed table above the mother culture dish or the sub-culture dish; when the conduit moves vertically, the collection structure will first squeeze the collection sac, and then the two will separate from each other to collect the fungal sample in the mother culture dish or release the fungal sample into the sub-culture dish; and the liquid replenishment structure will increase the flow rate of the liquid medicine in the medicine bottle into the mother culture dish to supplement the sample loss.

[0009] As a further scheme of the present invention: the transfer structure includes a turntable rotatably installed on the fixed sleeve; a first gear is fixedly installed on the turntable; an inclined groove is opened on the turntable; a translation straight groove matched with the inclined groove is opened on the installation frame; a sliding column that is slidably fitted with both the inclined groove and the translation straight groove is arranged on the installation frame; a chute is opened on the sliding column, and the chute is slidably fitted with the conduit; a baffle is fixedly installed on the conduit, and the baffle abuts against the sliding column.

[0010] As a further scheme of the present invention: the transfer structure further includes a fixed plate fixedly installed on the installation frame, a set of matching grooves is opened on the fixed plate, and a second protruding column that is slidably fitted with the set of matching grooves is fixedly installed on the sliding column; a telescopic sleeve is fixedly installed on the baffle; a guide rail is fixedly installed on the fixed plate; a slider is slidably fitted on the guide rail, and a telescopic column that is slidably fitted with the telescopic sleeve is fixedly installed on the slider; a spring is arranged inside the telescopic sleeve, and both ends of the spring abut against the slider and the baffle respectively.

[0011] As a further solution of the present invention: the mating groove group includes a moving straight groove and two groups of symmetrically arranged collecting vertical grooves and reset inclined grooves; one ends of the two groups of collecting vertical grooves are respectively communicated with two ends of the moving straight groove, one end of the reset inclined groove is communicated with the other end of the collecting vertical groove, and the other end thereof is communicated with the moving straight groove; a plurality of groups of limiting rods are rotatably installed on the fixing plate; a plurality of groups of limiting columns that are in contact and cooperation with the limiting rods are fixedly installed on the fixing plate.

[0012] As a further solution of the present invention: the collecting structure includes two groups of pressing structures fixedly installed on the fixing plate; the pressing structure includes two groups of symmetrically arranged wedge blocks; and a notch is provided on one of the wedge blocks; the wedge block is provided with an inclined slope and a vertical plane.

[0013] As a further solution of the present invention: the liquid supplementing structure includes a piston slidably fitted in the liquid storage tube; when the piston moves, it can block or conduct the liquid inlet hole.

[0014] As a further solution of the present invention: a motor is fixedly installed on the installation frame; a screw rod column is fixedly installed on the output end of the motor, and a threaded sleeve is threadedly connected to the screw rod column; a rotating shaft is rotatably installed on the installation frame; two groups of rotating groove groups connected end to end are provided on the rotating shaft; a moving sleeve fixedly connected to the threaded sleeve is sleeved on the rotating shaft, and a first protruding column slidably fitted with the rotating groove group is fixedly installed on the moving sleeve; the piston is fixedly connected to the threaded sleeve; a second gear meshing with the first gear is fixedly installed on the rotating shaft.

[0015] As a further solution of the present invention: the rotating groove group includes a first straight groove, a first inclined groove, a second straight groove and a second inclined groove; one end of the first straight groove is communicated with one end of the first inclined groove, the other end of the first inclined groove is communicated with one end of the second straight groove, the other end of the second straight groove is communicated with one end of the second inclined groove, the other end of the second inclined groove is communicated with one end of the first straight groove in the other group of rotating groove groups; the other end of the first straight groove is communicated with one end of the second inclined groove in the other group of rotating groove groups.

[0016] As a further solution of the present invention: the collecting tube is in a hourglass shape, and the inner diameter at the middle position is smaller than the inner diameters at both ends.

[0017] As a further solution of the present invention: the collecting bladder is made of rubber material and has good deformation and recovery capabilities.

[0018] Compared with the prior art, the beneficial effects of the present invention are as follows: Through the mutual cooperation of the collection structure and the transfer structure, the same conditions can be provided for the sub-dish process (the same collection method, the same collection environment, the same collection time point, etc.). Therefore, the survival status of fungi in each sub-culture dish is only related to the composition of the inhibitory drug, which can reduce the influence of external interference on the diagnosis process of fungal drug resistance, thereby improving the accuracy of diagnosis and facilitating the subsequent provision of the best treatment plan for patients; The fungal sample in the mother culture dish is collected by the collection structure squeezing the collection sac. The collection process is relatively gentle, which can reduce the damage to the fungi during the collection process, thereby improving the accurate diagnosis of fungal drug resistance; By driving the collection tube to move through the transfer structure, the movement trajectory can be accurately controlled, avoiding the fungi in the collection tube dripping on the fixed table or the rotating table during the movement process, and effectively ensuring the orderly progress of the fungal drug resistance diagnosis; And it can avoid increasing the cleaning difficulty of the subsequent device; By intermittently supplementing the consumption of nutrients in the mother culture dish through the dropper during the fungal mother culture process, the normal reproduction of the fungal mother is ensured, thereby improving the accuracy of the diagnosis of fungal strains. And during the collection process, the dripping speed of the dropper is increased through the liquid supplement structure to supplement the collection consumption in the mother culture dish, so as to facilitate the normal reproduction of the fungal mother in the mother culture dish after the collection, and provide a comparison sample for the subsequent treatment; Through the mutual cooperation of the transfer structure, the collection structure and the liquid injection structure, the transfer of the fungal sample to the sub-culture dishes containing different inhibitory drugs can be automatically completed without changing the internal environment of the incubator. Then, the drug resistance of the fungi is diagnosed by observing the survival status of the fungi. The diagnosis process is relatively rigorous, and the diagnosis result has higher research value. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 FIG. is a schematic structural diagram of an embodiment of a fungal culture device for skin infection diagnosis.

[0020] Figure 2 FIG. is a schematic structural diagram of a fixed table in an embodiment of a fungal culture device for skin infection diagnosis.

[0021] Figure 3 FIG. is a schematic structural diagram of a mounting frame in an embodiment of a fungal culture device for skin infection diagnosis.

[0022] Figure 4 FIG. is a schematic structural diagram of a rotating table in an embodiment of a fungal culture device for skin infection diagnosis.

[0023] Figure 5 FIG. is a schematic structural diagram of a medicine bottle in an embodiment of a fungal culture device for skin infection diagnosis.

[0024] Figure 6 FIG. is a schematic structural diagram of a first gear and a second gear in an embodiment of a fungal culture device for skin infection diagnosis.

[0025] Figure 7 is Figure 6 the structural schematic diagram of part A in

[0026] Figure 8 the structural schematic diagram of the dropper in an embodiment of a fungal culture device for skin infection diagnosis.

[0027] Figure 9 the structural schematic diagram of the wedge block in an embodiment of a fungal culture device for skin infection diagnosis.

[0028] Figure 10 is Figure 9 the structural schematic diagram of part B in

[0029] Figure 11 the structural schematic diagram of the sliding column in an embodiment of a fungal culture device for skin infection diagnosis.

[0030] In the figure: 1. Incubator; 2. Fixed platform; 3. Rotating platform; 4. Installation frame; 401. Translation straight groove; 402. Fixed sleeve; 5. Turntable; 501. Tilt groove; 502. First gear; 6. Medicine bottle; 7. Liquid storage tube; 701. Liquid inlet hole; 8. Dropper; 9. Motor; 10. Lead screw column; 11. Threaded sleeve; 12. Moving sleeve; 1201. First protruding column; 13. Rotating shaft; 1301. First straight groove; 1302. First inclined groove; 1303. Second straight groove; 1304. Second inclined groove; 1305. Second gear; 14. Sliding column; 1401. Second protruding column; 1402. Sliding groove; 15. Conduit; 1501. Baffle; 16. Collection sac; 17. Collection tube; 18. Fixed plate; 1801. Moving straight groove; 1802. Collection vertical groove; 1803. Reset inclined groove; 19. Slide block; 1901. Telescopic column; 20. Telescopic sleeve; 21. Spring; 22. Guide rail; 23. Limit rod; 24. Limit column; 25. Wedge block; 2501. Notch; 26. Mother culture dish; 27. Sub - culture dish 28. Piston. Detailed implementation manners

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] In addition, an element in the present invention is referred to as "fixed to" or "disposed on" another element, and it can be directly on the other element or there may also be an intermediate element. When an element is considered to be "connected" to another element, it can be directly connected to the other element or there may be an intermediate element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are only for illustrative purposes and do not represent the only implementation manners.

[0033] Please refer to Figures 1 to 11 , in the embodiment of the present invention, a fungal culture device for skin infection diagnosis includes an incubator 1; a fixed table 2 and an installation frame 4 are fixedly installed in the incubator 1, and a rotating table 3 is rotatably installed on the fixed table 2; a mother culture dish 26 and multiple groups of sub - culture dishes 27 are fitted on the fixed table 2 and the rotating table 3; A medicine bottle 6 and a liquid storage tube 7 are fixedly installed in the incubator 1; a liquid inlet hole 701 is opened on the liquid storage tube 7; and the medicine bottle 6 and the liquid storage tube 7 are communicated through the liquid inlet hole 701; a fixed sleeve 402 is fixedly installed in the installation frame 4, and a drip tube 8 communicated with the liquid storage tube 7 is fixedly installed in the fixed sleeve 402; the end of the drip tube 8 is located directly above the mother culture dish 26; Multiple groups of conduits 15 are slidably installed in the incubator 1, a collection tube 17 is clamped at one end of the conduit 15, and a collection sac 16 is fixedly installed at the other end; The incubator 1 is provided with a collection structure, a transfer structure and a liquid supplement structure that cooperate with each other; The transfer structure can drive the catheter 15 to move vertically and radially along the fixed platform 2 above the mother culture dish 26 or the sub-culture dish 27; when the catheter 15 moves vertically, the collection structure will first squeeze the collection sac 16, and then the two will separate from each other to collect the fungal sample in the mother culture dish 26 or release the fungal sample into the sub-culture dish 27; and the liquid replenishment structure will increase the flow rate of the liquid in the medicine bottle 6 into the mother culture dish 26 to supplement the sample loss.

[0034] Taking the embodiment combined with all the features described in this application as an example, when in use, the fungal sample collected from the patient's skin is placed in the mother culture dish 26, and the mother culture dish 26 is placed in the incubator 1 for fungal mother culture; the incubator 1 can provide suitable environmental requirements such as temperature, humidity, oxygen content, etc. for fungal culture.

[0035] The medicine bottle 6 is loaded with the necessary nutrients during the fungal culture process. The liquid in the medicine bottle 6 will first flow into the liquid storage tube 7 through the liquid inlet hole 701, and after storing a certain water head height in the liquid storage tube 7, it will slowly drip into the mother culture dish 26 through the dropper tube 8 for the use of fungal colony reproduction (to supplement the nutrients consumed in the mother culture dish 26 during the fungal reproduction process).

[0036] After a period of time, when the fungi in the mother culture dish 26 have reproduced to form fungal colonies sufficient for diagnosis (at this time, fungal species research can be carried out through experiments), the fungi in the mother culture dish 26 are collected and released into the sub-culture dish 27 through the transfer structure and the collection structure; the sub-culture dish 27 contains the necessary nutrients for the survival of fungi, and each sub-culture dish 27 contains different components of drugs (drug components that inhibit the survival of fungi). By observing the different survival performances of the fungi in each sub-culture dish 27, the drug resistance of the fungi can be further judged.

[0037] When the transfer structure operates, it will first drive the catheter 15 to move radially along the mounting frame 4 close to the directly above of the mother culture dish 26, thereby driving the collection tube 17 to move synchronously; and when the collection tube 17 moves to the end of the stroke, the catheter 15 will drive the collection tube 17 to move vertically close to the mother culture dish 26. During this process, the collection tube 17 will gradually insert into the mother culture dish 26, thereby dividing the fungal colony.

[0038] During the vertical movement of the collection tube 17, the collection structure will first compress the collection sac 16 to discharge the air inside (during this process, the collection tube 17 does not contact the fungal colony), and then the collection structure will separate from the collection sac 16, and the collection sac 16 will reset, so that the collection tube 17 will absorb the divided fungal colony into the collection tube 17; Subsequently, driven by the transfer structure, the conduit 15 moves vertically to drive the collection tube 17 away from the mother culture dish 26; then the conduit 15 moves towards the daughter culture dish 27 to drive the collection tube 17 to move synchronously; when the conduit 15 moves to the end of its stroke, the conduit 15 moves vertically to drive the collection tube 17 closer to the daughter culture dish 27. During the approaching process, the collection structure squeezes the collection sac 16, thereby injecting the fungal colonies collected in the collection tube 17 into the daughter culture dish 27 (the height of the daughter culture dish 27 in the vertical direction is lower than that of the mother culture dish 26).

[0039] Meanwhile, the liquid supplement structure increases the flow rate of the liquid medicine in the liquid storage tube 7 to accelerate the liquid supplement speed of the dropper 8 into the mother culture dish 26, thereby replenishing the components consumed in the mother culture dish 26 during the subculture process, so as not to affect the living environment of the mother in the mother culture dish 26.

[0040] Through the mutual cooperation of the collection structure and the transfer structure, the same conditions can be provided for the subculture process (the same collection method, the same collection environment, the same collection time point, etc.). Therefore, the survival status of the fungi in each daughter culture dish 27 is only related to the composition of the inhibitory drug, which can reduce the influence of external interference on the fungal drug resistance diagnosis process, thereby improving the accuracy of the diagnosis and facilitating the subsequent provision of the best treatment plan for the patient; the fungal sample in the mother culture dish 26 is collected by the collection structure squeezing the collection sac 16, and the collection process is relatively gentle, which can reduce the damage to the fungi during the collection process, thereby improving the accurate diagnosis of fungal drug resistance; the transfer structure drives the collection tube 17 to move, which can accurately control the movement trajectory, avoid the fungi in the collection tube 17 falling on the fixed table 2 or the rotating table 3 during the movement process, and can effectively ensure the orderly progress of the fungal drug resistance diagnosis; and it can avoid increasing the cleaning difficulty of the subsequent device; and by intermittently supplementing the consumption of nutrients in the mother culture dish 26 during the fungal mother culture process by the dropper 8, the normal reproduction of the fungal mother is ensured, thereby improving the accuracy of the fungal strain diagnosis. Moreover, during the collection process, the liquid supplement structure increases the dripping speed of the dropper 8 to supplement the collection consumption in the mother culture dish 26, so as to facilitate the normal reproduction of the fungal mother in the mother culture dish 26 after the collection, and provide a comparison sample for the subsequent treatment.

[0041] In another embodiment of the present invention, the transfer structure includes a turntable 5 rotatably mounted on the fixed sleeve 402; a first gear 502 is fixedly mounted on the turntable 5; an inclined groove 501 is formed on the turntable 5; a translation straight groove 401 is formed on the mounting frame 4 and is matched with the inclined groove 501; a sliding column 14 is arranged on the mounting frame 4 and is slidably fitted with both the inclined groove 501 and the translation straight groove 401; a chute 1402 is formed on the sliding column 14, and the chute 1402 is slidably fitted with the conduit 15; a baffle 1501 is fixedly mounted on the conduit 15, and the baffle 1501 abuts against the sliding column 14.

[0042] Taking the embodiment combined with all the features described in this application as an example, when in use, when the first gear 502 rotates, it will drive the turntable 5 to rotate, thereby driving the inclined groove 501 to rotate synchronously; the groove wall of the rotating inclined groove 501 will squeeze the sliding column 14, so that the sliding column 14 can slide in the translation straight groove 401, enabling the sliding column 14 to reciprocally translate between the mother culture dish 26 and the sub-culture dish 27.

[0043] During the translation of the sliding column 14, the conduit 15 will slide in the chute 1402, and when the groove wall at one end of the chute 1402 abuts against the conduit 15, the sliding column 14 will drive the conduit 15 to move synchronously, thereby driving the collection tube 17 to move.

[0044] After the sliding column 14 moves to the end point of the translation straight groove 401, the sliding column 14 will move in the vertical direction, thereby driving the conduit 15 to move synchronously through the baffle 1501, so as to approach or move away from the mother culture dish 26 and the sub-culture dish 27, thereby realizing the collection and injection of the fungal mother.

[0045] And when the conduit 15 slides in the chute 1402, the sliding column 14 will drive the conduit 15 away from the mother culture dish 26 or the sub-culture dish 27, so as to avoid the collection tube 17 from being collided.

[0046] Through the mutual cooperation of the inclined groove 501 and the translation straight groove 401, the moving direction of the sliding column 14 can be controlled by controlling the rotation direction of the turntable 5, so as to control the collection tube 17 to move from the mother culture dish 26 to the sub-culture dish 27 or from the sub-culture dish 27 to the mother culture dish 26, thereby realizing the collection and transfer of the fungal sample.

[0047] In another embodiment of the present invention, the transfer structure also includes a fixed plate 18 fixedly mounted on the mounting frame 4, a mating groove group being provided on the fixed plate 18, a second protruding column 1401 being fixedly mounted on the sliding column 14 and slidably engaged with the mating groove group; a telescopic sleeve 20 is fixedly mounted on the baffle 1501; a guide rail 22 is fixedly mounted on the fixed plate 18; a slider 19 is slidably engaged on the guide rail 22, a telescopic column 1901 being slidably engaged with the telescopic sleeve 20 is fixedly mounted on the slider 19; a spring 21 is arranged inside the telescopic sleeve 20, and both ends of the spring 21 respectively contact the slider 19 and the baffle 1501.

[0048] In another embodiment of the present invention, the mating slot group includes a movable straight slot 1801 and two symmetrically arranged groups of collecting vertical slots 1802 and a reset inclined slot 1803; wherein one end of the two groups of collecting vertical slots 1802 are respectively connected to the two ends of the movable straight slot 1801, one end of the reset inclined slot 1803 is connected to the other end of the collecting vertical slot 1802, and the other end thereof is connected to the movable straight slot 1801; a plurality of groups of limiting rods 23 are rotatably installed on the fixed plate 18; a plurality of groups of limiting columns 24 that are in contact with and cooperate with the limiting rods 23 are fixedly installed on the fixed plate 18.

[0049] Taking the embodiment combining all the features described in the present application as an example, when in use, in the initial position, the second protruding column 1401 is located in the middle part of the movable straight groove 1801; at this time, the collection tube 17 is located between the mother culture dish 26 and the daughter culture dish 27. This position can avoid the difficulty of placing the mother culture dish 26 on the fixed table 2 and avoid the device from being damaged by impact; and at this time, the spring 21 is in a compressed state.

[0050] During the collection process, the turntable 5 is rotated forward to drive the sliding column 14 to move toward the mother culture dish 26 through the cooperation between the inclined groove 501 and the translation straight groove 401; thereby driving the slider 19 to slide in the guide rail 22 through the guide tube 15.

[0051] During this process, the second protruding column 1401 will slide in the moving straight groove 1801 toward the end close to the mother culture dish 26; when the second protruding column 1401 slides to the connection between the moving straight groove 1801 and the collection vertical groove 1802 (when the second protruding column 1401 moves to the connection between the moving straight groove 1801 and the reset inclined groove 1803, under the mutual interference and cooperation of the limiting rod 23 and the limiting column 24, the second protruding column 1401 can smoothly move to the collection vertical groove 1802 Sliding), under the elastic force of the spring 21, the second protruding column 1401 will slide in the collection vertical groove 1802, and at the same time, the telescopic column 1901 will slide outward in the telescopic sleeve 20; thereby driving the catheter 15 to move synchronously, so that the collection tube 17 approaches the fungal mother plant; and in the approaching process, the collection structure will first compress the collection capsule 16, and after the collection tube 17 contacts the fungal mother plant, it will break away from the contact with the collection capsule 16, so that the fungus is collected by resetting the collection capsule 16.

[0052] Then, the turntable 5 is rotated in the reverse direction to drive the sliding column 14 to move to the initial position. At this time, the second protruding column 1401 will slide in the reset inclined groove 1803 (when the second protruding column 1401 moves to the connection end of the reset inclined groove 1803 and the movable straight groove 1801, the second protruding column 1401 will squeeze the limiting rod 23 to make the limiting rod 23 rotate, so that the second protruding column 1401 can smoothly enter the movable straight groove 1801, and the limiting rod 23 will be separated from the second protruding column 1401. After contact, it is reset under the action of the torsion spring), thereby driving the sliding column 14 to move obliquely upward; during this process, the catheter 15 will slide in the slide groove 1402, and the inclined upward sliding column 14 will drive the catheter 15 to move upward, thereby driving the collection tube 17 to separate from the fungal mother plant; at this time, the catheter 15 does not move in the horizontal direction until the groove wall of the slide groove 1402 conflicts with the catheter 15, and the sliding column 14 will drive the catheter 15 to move synchronously, thereby driving the collection tube 17 loaded with the fungal sample to move synchronously.

[0053] During the release process, the turntable 5 is rotated in the opposite direction to drive the sliding column 14 to move toward the sub-culture dish 27 through the cooperation between the inclined groove 501 and the translation straight groove 401; thereby driving the slider 19 to slide in the guide rail 22 through the guide tube 15.

[0054] During this process, the second protruding column 1401 will slide in the movable straight groove 1801 toward one end close to the sub-culture dish 27; when the second protruding column 1401 slides to the connection between the movable straight groove 1801 and the collection vertical groove 1802 (when the second protruding column 1401 moves to the connection between the movable straight groove 1801 and the reset inclined groove 1803, under the mutual interference and cooperation between the limiting rod 23 and the limiting column 24, the second protruding column 1401 can smoothly slide toward the collection vertical groove 1802), under the elastic force of the spring 21, the second protruding column 1401 will slide in the collection vertical groove 1802, and at the same time, the telescopic column 1901 will slide outward in the telescopic sleeve 20; thereby driving the catheter 15 to move synchronously, so that the collection tube 17 approaches the sub-culture dish 27; and in the approaching process, the collection structure will first compress the collection capsule 16, thereby injecting the fungal sample into the sub-culture dish 27.

[0055] Then, the turntable 5 is rotated forward to drive the sliding column 14 to move toward the initial position. At this time, the second protruding column 1401 will slide in the reset inclined groove 1803, thereby driving the sliding column 14 to move obliquely upward; during this process, the catheter 15 will slide in the slide groove 1402, and the inclined upward sliding column 14 will drive the catheter 15 to move upward, thereby driving the collection tube 17 away from the sub-culture dish 27; at this time, the catheter 15 does not move in the horizontal direction until the groove wall of the slide groove 1402 conflicts with the catheter 15, and the sliding column 14 will drive the catheter 15 to move synchronously, thereby driving the collection tube 17 to reset.

[0056] Through the cooperation between the collection structure and the transfer structure, the same conditions (same collection method, same collection environment, same collection time point, etc.) can be provided for the sub-dish process. Therefore, the survival status of the fungi in each sub-dish 27 is only related to the components of the inhibitory drug, which can reduce the impact of external interference on the fungal resistance diagnosis process, thereby improving the accuracy of the diagnosis and facilitating the subsequent provision of the best treatment plan for the patient; the transfer structure drives the collection tube 17 to move, and the movement trajectory can be accurately controlled to prevent the fungi in the collection tube 17 from dripping onto the fixed table 2 or the rotating table 3 during the movement, which can effectively ensure the orderly progress of the fungal resistance diagnosis; and can avoid increasing the difficulty of subsequent device cleaning.

[0057] In another embodiment of the present invention, the collection structure includes two groups of extrusion structures fixedly mounted on the fixed plate 18; the extrusion structure includes two groups of symmetrically arranged wedge blocks 25; and one of the wedge blocks 25 is provided with a notch 2501; and the wedge block 25 is provided with an inclined slope and a vertical plane.

[0058] Taking the embodiment combining all the features described in this application as an example, when in use, during the process of the second protruding column 1401 moving in the moving straight groove 1801, it will drive the catheter 15 to move horizontally. And when the second protruding column 1401 moves to the end point of the moving straight groove 1801, the catheter 15 will enter between the two wedge blocks 25 from the notch 2501.

[0059] The distance between the planes of the two wedge blocks 25 is smaller than the diameter of the collection sac 16.

[0060] When the second protruding column 1401 slides in the collection vertical groove 1802, it will drive the collection sac 16 to move towards the fixed platform 2. The collection sac 16 will first contact the slope surface of the wedge block 25, and under the action of the slope surface, the collection sac 16 has a tendency to be compressed.

[0061] After that, the collection sac 16 will enter between the two wedge blocks 25, and the collection sac 16 will be squeezed by the vertical planes of the wedge blocks 25, thereby compressing the collection sac 16 to discharge the air in the collection sac 16 and push out the fungal sample in the collection tube 17.

[0062] After that, the collection sac 16 will pass through the wedge blocks 25, that is, the wedge blocks 25 will be separated from the collection sac 16. At this time, the collection sac 16 will recover, thereby sucking the fungus into the collection tube 17. By squeezing the collection sac 16 through the collection structure to collect the fungal sample in the mother culture dish 26, the collection process is relatively gentle, which can reduce the damage to the fungus during the collection process, thereby improving the accurate diagnosis of the fungal drug resistance.

[0063] In another embodiment of the present invention, the liquid supplement structure includes a piston 28 slidably fitted in the liquid storage tube 7; when the piston 28 moves, it can close or conduct the liquid inlet hole 701.

[0064] Taking the embodiment combining all the features described in this application as an example, when in use, during the process of the transfer structure and the collection structure cooperating with each other to transfer the fungal sample in the mother culture dish 26 to the sub-culture dish 27 in a divided manner, the piston 28 will move in the liquid storage tube 7 towards the dropper 8.

[0065] In the initial position, the piston 28 is separated from the liquid inlet hole 701, so that the liquid medicine in the medicine bottle 6 can flow into the liquid storage tube 7 and slowly drip through the dropper 8.

[0066] During the movement of the piston 28, the piston 28 will gradually contact the liquid inlet hole 701, thereby blocking the liquid inlet hole 701 to prevent the liquid medicine from flowing back; and under the extrusion of the piston 28, the liquid medicine stored in the liquid storage tube 7 will be pushed into the dropper 8, thereby accelerating the flow of the liquid medicine in the dropper 8 to supplement the collection loss in the mother culture dish 26; to facilitate the normal reproduction of the fungal parent in the mother culture dish 26 after the collection and provide a comparison sample for subsequent treatment.

[0067] In another embodiment of the present invention, a motor 9 is fixedly installed on the installation frame 4; a lead screw column 10 is fixedly installed on the output end of the motor 9, and a threaded sleeve 11 is threadedly connected to the lead screw column 10; a rotating shaft 13 is rotatably installed on the installation frame 4; two sets of rotating groove groups connected end to end are formed on the rotating shaft 13; a moving sleeve 12 fixedly connected to the threaded sleeve 11 is sleeved on the rotating shaft 13, and a first protruding column 1201 slidably fitted with the rotating groove group is fixedly installed on the moving sleeve 12; the piston 28 is fixedly connected to the threaded sleeve 11; a second gear 1305 meshing with the first gear 502 is fixedly installed on the rotating shaft 13.

[0068] In another embodiment of the present invention, the rotating groove group includes a first straight groove 1301, a first inclined groove 1302, a second straight groove 1303, and a second inclined groove 1304; one end of the first straight groove 1301 is communicated with one end of the first inclined groove 1302, the other end of the first inclined groove 1302 is communicated with one end of the second straight groove 1303, the other end of the second straight groove 1303 is communicated with one end of the second inclined groove 1304, and the other end of the second inclined groove 1304 is communicated with one end of the first straight groove 1301 in another set of the rotating groove groups; the other end of the first straight groove 1301 is communicated with one end of the second inclined groove 1304 in another set of the rotating groove groups.

[0069] Taking the embodiment combined with all the features described in this application as an example, when the fungi in the mother culture dish 26 multiply to a suitable stage for sub-dish division, the drug resistance diagnosis of the fungi is carried out.

[0070] Start the motor 9 to drive the lead screw column 10 to rotate forward, and drive the threaded sleeve 11 to move along the length direction of the lead screw column 10 towards the fixed table 2 through thread cooperation. At this time, the piston 28 will move synchronously with the threaded sleeve 11 to gradually inject the liquid medicine stored in the liquid storage tube 7 into the mother culture dish 26.

[0071] At the same time, the moving sleeve 12 will also move synchronously with the threaded sleeve 11 to drive the first protruding column 1201 to slide in the rotating groove group.

[0072] When the first protruding column 1201 moves in the first straight groove 1301, the rotating shaft 13 does not rotate at this time.

[0073] When the first protruding column 1201 slides in the first inclined groove 1302, under the extrusion of the first protruding column 1201 and the groove wall of the first inclined groove 1302, the rotating shaft 13 will rotate, thereby driving the second gear 1305 to rotate, and driving the first gear 502 to rotate forward through meshing, so as to drive the second protruding column 1401 to slide to the connection with the collection vertical groove 1802 in the moving straight groove 1801.

[0074] When the first protruding column 1201 slides in the second straight groove 1303 , the rotating shaft 13 does not rotate, and during this process, the second protruding column 1401 slides in the collection vertical groove 1802 .

[0075] When the first protruding column 1201 slides in the second inclined groove 1304, the rotating shaft 13 will rotate under the squeezing effect of the first protruding column 1201 and the groove wall of the second inclined groove 1304, thereby driving the second gear 1305 to rotate, and driving the first gear 502 to rotate in the opposite direction through the meshing action, thereby driving the second protruding column 1401 to slide in the reset inclined groove 1803 and the movable straight groove 1801, so that the sliding column 14 is reset.

[0076] When the sliding column 14 completes the reset, the threaded sleeve 11 moves to the end of the stroke. At this time, the motor 9 will drive the screw column 10 to rotate in the opposite direction, so as to cooperate with the threaded sleeve 11 to move in the opposite direction, thereby driving the piston 28 to reset, so that the liquid inlet hole 701 is conductive, and driving the movable sleeve 12 to move in the opposite direction, thereby driving the first protruding column 1201 to slide in another set of rotating grooves, so as to inject the fungal sample in the collection tube 17 into the sub-culture dish 27 through the transfer structure and the collection structure.

[0077] Through the cooperation among the transfer structure, the collection structure and the injection structure, the fungal sample can be automatically transferred to the sub-culture dish 27 containing different inhibitory drugs without changing the internal environment of the incubator 1. Then, the drug resistance of the fungus can be diagnosed by observing the survival status of the fungus. The diagnosis process is relatively rigorous and the diagnosis results have higher research value.

[0078] In another embodiment of the present invention, the collection tube 17 is hourglass-shaped, and the inner diameter in the middle is smaller than the inner diameters at both ends.

[0079] Taking the embodiment combining all the features recorded in the present application as an example, when in use, during the recovery process of the collection capsule 16, part of the colonies in the mother culture dish 26 will be absorbed into the collection tube 17; the inner diameter of the middle part of the collection tube 17 is smaller, which can effectively prevent the sample from dripping during the transfer process.

[0080] In another embodiment of the present invention, the collection bag 16 is made of rubber material and has good deformation and recovery capabilities.

[0081] Taking the embodiment combining all the features described in the present application as an example, when in use, the recovery ability of the collection capsule 16 is relatively strong, and the suction force generated during the recovery process of the collection capsule 16 is relatively stable, thereby avoiding collection failure.

[0082] It is apparent to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential characteristics of the present invention. Therefore, in all respects, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Accordingly, all changes that fall within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present invention. Any reference signs in the claims should not be construed as limiting the claims involved.

[0083] In addition, it should be understood that although this specification is described in terms of embodiments, not every embodiment contains only one independent technical solution. This narrative manner of the specification is merely for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in the various embodiments can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A fungal culture device for skin infection diagnosis, comprising an incubator; a fixed table and an installation frame are fixedly installed inside the incubator, and a rotating table is rotatably installed on the fixed table; a mother culture dish and multiple groups of sub-culture dishes are fitted on the fixed table and the rotating table; It is characterized in that A medicine bottle and a liquid storage tube are fixedly installed inside the incubator; a liquid inlet hole is opened on the liquid storage tube; and the medicine bottle and the liquid storage tube are communicated through the liquid inlet hole; a fixed sleeve is fixedly installed inside the installation frame, and a dropper tube communicated with the liquid storage tube is fixedly installed inside the fixed sleeve; the end of the dropper tube is located directly above the mother culture dish; Multiple groups of conduits are slidably installed inside the incubator, a collection tube is clamped on one end of the conduit, and a collection sac is fixedly installed on the other end; A collection structure, a transfer structure and a liquid replenishment structure which cooperate with each other are arranged on the incubator; The transfer structure can drive the conduit to move vertically and radially along the fixed table above the mother culture dish or the sub-culture dish; when the conduit moves vertically, the collection structure will first squeeze the collection sac, and then the two will be separated from contact to collect the fungal sample in the mother culture dish or release the fungal sample into the sub-culture dish; and the liquid replenishment structure will increase the flow rate of the liquid medicine in the medicine bottle into the mother culture dish to supplement the sample loss.

2. The fungal culture device for skin infection diagnosis according to claim 1, wherein The transfer structure includes a turntable rotatably installed on the fixed sleeve; a first gear is fixedly installed on the turntable; an inclined groove is opened on the turntable; a translation straight groove matched with the inclined groove is opened on the installation frame; a sliding column which is slidably fitted with both the inclined groove and the translation straight groove is arranged on the installation frame; a chute is opened on the sliding column, and the chute is slidably fitted with the conduit; a baffle is fixedly installed on the conduit, and the baffle abuts against the sliding column.

3. The fungal culture device for skin infection diagnosis according to claim 2, wherein The transfer structure further includes a fixing plate fixedly installed on the installation frame, a set of matching groove groups are opened on the fixing plate, and a second protruding column slidably fitted with the set of matching groove groups is fixedly installed on the sliding column; a telescopic sleeve is fixedly installed on the baffle; a guide rail is fixedly installed on the fixing plate; a slider is slidably fitted on the guide rail, and a telescopic column slidably fitted with the telescopic sleeve is fixedly installed on the slider; a spring is arranged inside the telescopic sleeve, and two ends of the spring abut against the slider and the baffle respectively.

4. The fungal culture device for skin infection diagnosis according to claim 3, wherein, The set of matching groove groups includes a moving straight groove and two sets of symmetrically arranged collection vertical grooves and reset inclined grooves; one ends of the two sets of collection vertical grooves are respectively communicated with two ends of the moving straight groove, one end of the reset inclined groove is communicated with the other end of the collection vertical groove, and the other end of the reset inclined groove is communicated with the moving straight groove; multiple groups of limiting rods are rotatably installed on the fixing plate; multiple groups of limiting columns which are in abutting cooperation with the limiting rods are fixedly installed on the fixing plate.

5. The fungal culture device for skin infection diagnosis according to claim 3, characterized in that, The collection structure includes two sets of extrusion structures fixedly installed on the fixing plate; each extrusion structure includes two sets of symmetrically arranged wedge blocks; and a notch is opened on one of the wedge blocks; an inclined slope and a vertical plane are arranged on the wedge block.

6. The fungal culture device for skin infection diagnosis according to claim 2, characterized in that, The liquid replenishing structure includes a piston slidably fitted in the liquid storage tube; when the piston moves, it can close or conduct the liquid inlet hole.

7. A fungal culture device for skin infection diagnosis according to claim 6, wherein, A motor is fixedly installed on the installation frame; a lead screw column is fixedly installed on the output end of the motor, and a threaded sleeve is threadedly connected to the lead screw column; a rotating shaft is rotatably installed on the installation frame; two sets of rotating groove groups connected end to end are formed on the rotating shaft; a moving sleeve fixedly connected to the threaded sleeve is sleeved on the rotating shaft, and a first protruding column slidably fitted with the rotating groove group is fixedly installed on the moving sleeve; the piston is fixedly connected to the threaded sleeve; a second gear meshing with the first gear is fixedly installed on the rotating shaft.

8. A fungal culture device for skin infection diagnosis according to claim 7, characterized in that, The rotating groove group includes a first straight groove, a first inclined groove, a second straight groove and a second inclined groove; one end of the first straight groove communicates with one end of the first inclined groove, the other end of the first inclined groove communicates with one end of the second straight groove, the other end of the second straight groove communicates with one end of the second inclined groove, the other end of the second inclined groove communicates with one end of the first straight groove in another set of the rotating groove groups; the other end of the first straight groove communicates with one end of the second inclined groove in another set of the rotating groove groups.

9. The fungal culture device for skin infection diagnosis according to claim 1, characterized in that, The collection tube is in an hourglass shape, and the inner diameter at the middle position is smaller than the inner diameters at both ends.

10. The fungal culture device for skin infection diagnosis according to claim 1, characterized in that, The collection bladder is made of rubber material and has good deformation and recovery capabilities.

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