Sample analyzer

Abstract

The invention is applicable to the field of in-vitro diagnostic equipment, and discloses a sample analyzer. The sample analyzer comprises a sample distribution device, a nucleic acid extraction device, a pipetting device, an amplification device and a detection device, the nucleic acid extraction device comprises a first functional component, a second functional component and a transfer component, and the first functional component is arranged above or below the second functional component; the transfer assembly is used for transferring the nucleic acid extraction container between the first functional assembly and the second functional assembly, the first functional assembly is used for at least carrying out magnetic adsorption and liquid absorption actions on liquid in the nucleic acid extraction container, and the second functional assembly is at least used for incubating the liquid in the nucleic acid extraction container. The first functional component and the second functional component which are respectively used for bearing the nucleic acid extraction container to execute functional actions in the nucleic acid extraction device are arranged to be distributed up and down, so that the horizontal space occupied by the nucleic acid extraction device is reduced, and the miniaturization design of a sample analyzer is facilitated.

Description

Technical Field

[0001] The present invention relates to the field of in vitro diagnostic devices, and particularly to a sample analyzer. Background Art

[0002] Molecular diagnostic technology refers to a technology that uses DNA and RNA as diagnostic materials and uses molecular biology techniques to detect the presence, defects, or abnormal expression of genes, thereby diagnosing the human body's state and diseases. Its basic principle is to detect whether the structure of DNA or RNA changes, the amount, and whether the expression function is abnormal, so as to determine whether there are abnormal changes at the gene level in the tested person, which is of great significance for the prevention, prediction, diagnosis, treatment, and prognosis of diseases. A molecular diagnostic all-in-one machine is a detection device for realizing molecular diagnostic detection. Because molecular diagnosis requires multiple operation steps such as sample preparation, nucleic acid extraction, and reaction system construction, there are many functional modules in the corresponding molecular diagnostic all-in-one machine. Moreover, in order to achieve complete sample input and result output, there are also many auxiliary functional modules such as reagent management, waste management, and anti-pollution management. The all-in-one machine composed of so many modules integrated together often has a very large external dimension. And for the molecular laboratory where the molecular diagnostic all-in-one machine is placed, due to the requirement of preventing pollution, it is necessary to achieve partition isolation and ensure a negative-pressure experimental environment. Therefore, the overall area of the current molecular laboratory is generally not large, so it can be said that every inch of land is precious. Placing a large-volume all-in-one machine in the molecular laboratory is a huge problem that users must face. For the manufacturers of molecular all-in-one machines, it is also imperative to reduce the volume of the machine.

[0003] In a molecular diagnostic all-in-one machine provided by the related technology, multiple workstations of the nucleic acid extraction device are arranged in a pipeline manner along the horizontal direction in sequence. In this way, the volume and the occupied horizontal space of the nucleic acid extraction device are very large, seriously affecting the miniaturization design of the molecular diagnostic all-in-one machine. The nucleic acid extraction device is one of the functional modules that occupy the largest space in the molecular diagnostic all-in-one machine. Optimizing its design and structural layout is of great significance in the compact design of the entire molecular diagnostic all-in-one machine. Summary of the Invention

[0004] The first object of the present invention is to provide a sample analyzer, which aims to solve the technical problem that the nucleic acid extraction device in the related technology occupies a large horizontal space.

[0005] To achieve the above object, the solution provided by the present invention is: A sample analyzer, comprising:

[0006] A sample dispensing device, which is used to aspirate a sample from a sample container and dispense at least part of the aspirated sample into a nucleic acid extraction container;

[0007] A nucleic acid extraction device, which is used to extract nucleic acids from a liquid in a nucleic acid extraction container that at least contains a sample and a reagent to obtain a nucleic acid extract, and the reagent contains magnetic beads;

[0008] A pipetting device, which is used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0009] An amplification device, which is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0010] A detection device, which is used to detect the test solution;

[0011] Wherein, the nucleic acid extraction device includes a first functional component, a second functional component and a transfer component. The first functional component is arranged above or below the second functional component. The transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component to perform the nucleic acid extraction to obtain the nucleic acid extract. The first functional component is used to at least perform magnetic adsorption and liquid suction actions on the liquid in the nucleic acid extraction container, and the second functional component is at least used to incubate the liquid in the nucleic acid extraction container.

[0012] As an implementation manner, the first functional component includes a first carrier member, a first pipetting member and a first magnetic attraction member. The first carrier member is used to carry the nucleic acid extraction container. The first magnetic attraction member is used to perform a first magnetic adsorption on the liquid in the nucleic acid extraction container located on the first carrier member that at least contains a sample and a reagent to adsorb the magnetic beads in the nucleic acid extraction container. The first pipetting member is used to perform a first liquid suction action on the liquid in the nucleic acid extraction container located on the first carrier member and subjected to the first magnetic adsorption by the first magnetic attraction member. The transfer component is used to transfer the nucleic acid extraction container between the first carrier member and the second functional component. The magnetic adsorption includes the first magnetic adsorption, and the liquid suction action includes the first liquid suction action.

[0013] As an implementation manner, the first functional component further includes a liquid injection member, and the liquid injection member is used to perform a first liquid injection action of injecting a cleaning solution into the nucleic acid extraction container located on the first carrier member.

[0014] As an implementation manner, the first functional component further includes a second carrier member, a second liquid transfer member, and a second magnetic attraction member. The second carrier member is used to carry the nucleic acid extraction container. The second magnetic attraction member is used to perform a second magnetic adsorption on the liquid containing at least a cleaning solution in the nucleic acid extraction container located on the second carrier member. The second liquid transfer member is used to perform a second liquid suction action on the liquid in the nucleic acid extraction container located on the second carrier member and subjected to the second magnetic adsorption by the second magnetic attraction member. The transfer component is further used to transfer the nucleic acid extraction container between the second carrier member and the second functional component. The magnetic adsorption further includes the second magnetic adsorption, and the liquid suction action further includes the second liquid suction action.

[0015] As an implementation manner, the first functional component further includes a third carrier member and a third liquid transfer member. The third carrier member is used to carry the nucleic acid extraction container. The third liquid transfer member is used to perform a second liquid injection action of injecting an elution reagent into the nucleic acid extraction container located on the third carrier member. The transfer component is further used to transfer the nucleic acid extraction container between the third carrier member and the second functional component.

[0016] As an implementation manner, the sample analyzer further includes a first lifting member. The first lifting member is connected to the second magnetic attraction member, and the first lifting member is used to drive the second magnetic attraction member to move up and down relative to the nucleic acid extraction container.

[0017] As an implementation manner, the nucleic acid extraction container is formed with a reaction hole, a first pipette tip hole, and a second pipette tip hole. The reaction hole is used to carry a sample and a reagent for nucleic acid extraction. The first pipette tip hole is used to carry a first liquid transfer pipette tip. The second pipette tip hole is used to carry a second liquid transfer pipette tip. The volume of the first liquid transfer pipette tip is larger than the volume of the second liquid transfer pipette tip.

[0018] The first liquid transfer member includes a first pipette tip connection component and a first liquid suction and discharge power component. The first pipette tip connection component is used to be detachably connected to the first liquid transfer pipette tip to suck liquid through the first liquid transfer pipette tip under the action of the power provided by the first liquid suction and discharge power component.

[0019] The third liquid transfer member includes a second pipette tip connection component and a second liquid suction and discharge power component. The second pipette tip connection component is used to be detachably connected to the second liquid transfer pipette tip to suck liquid through the second liquid transfer pipette tip under the action of the power provided by the second liquid suction and discharge power component.

[0020] As an implementation manner, the nucleic acid extraction container is further formed with a waste liquid hole. The first liquid transfer member and the second liquid transfer member are further used to discharge the liquid sucked from the reaction hole to the waste liquid hole.

[0021] As an implementation manner, the number of the first bearing members is at least two, and at least one liquid injection member and at least one first liquid transfer member are correspondingly arranged for each of the first bearing members.

[0022] As an implementation manner, the first bearing member, the second bearing member, and the third bearing member are arranged side by side in the horizontal direction.

[0023] As an implementation manner, the second functional component includes a fourth bearing member and a heating member. The fourth bearing member is used for bearing the nucleic acid extraction container to perform an incubation action, and the heating member is used for heating at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth bearing member; the fourth bearing member includes a lysis area, a washing area, a drying area, and an elution area. Accommodation cavities for placing the nucleic acid extraction container are respectively formed in the lysis area, the washing area, the drying area, and the elution area. The transfer assembly is used for transferring the nucleic acid extraction container between the lysis area, the washing area and the first bearing member, between the washing area, the drying area and the second bearing member, and between the drying area, the elution area and the third bearing member.

[0024] As an implementation manner, the fourth bearing member includes an incubation tray. The heating member is used for heating the nucleic acid extraction container on the incubation tray; the incubation tray forms the lysis area, the washing area, the drying area, and the elution area, and a first transfer station is formed on the incubation tray. The incubation tray is used for scheduling the nucleic acid extraction containers located in the lysis area, the washing area, the drying area, and the elution area to the first transfer station respectively, and the transfer assembly is used for scheduling the nucleic acid extraction container located at the first transfer station to the first functional component.

[0025] As an implementation manner, the sample analyzer further includes a controller, and the controller is configured to:

[0026] Control the transfer assembly to schedule the nucleic acid extraction container after performing the incubation action in the lysis area to the first bearing member;

[0027] Control the first liquid transfer member to perform the first liquid suction action on the liquid in the nucleic acid extraction container on the first bearing member and control the liquid injection member to perform the first liquid injection action on the nucleic acid extraction container on the first bearing member;

[0028] Control the transfer assembly to transfer the nucleic acid extraction container after performing the first liquid injection action to the washing area to perform the incubation action;

[0029] Control the transfer component to transfer the nucleic acid extraction container after performing the incubation action in the washing area to the second carrier member;

[0030] Control the second pipetting member to perform the second liquid suction action on the liquid in the nucleic acid extraction container in the second carrier member;

[0031] Control the transfer component to transfer the nucleic acid extraction container after performing the second liquid suction action to the drying area to perform a drying action on the nucleic acid extraction container;

[0032] Control the transfer component to transfer the nucleic acid extraction container after performing the drying action in the drying area to the third carrier member;

[0033] Control the third pipetting member to perform the second liquid injection action on the nucleic acid extraction container in the third carrier member;

[0034] Control the transfer component to transfer the nucleic acid extraction container after performing the second liquid injection action to the elution area to perform an elution action.

[0035] As an implementation manner, the position of the bottom of the accommodation cavity in the elution area is higher than the position of the bottom of the accommodation cavity in the lysis area, higher than the position of the bottom of the accommodation cavity in the washing area, and higher than the position of the bottom of the accommodation cavity in the drying area; and / or,

[0036] The position of the bottom of the accommodation cavity in the lysis area is at the same height as the position of the bottom of the accommodation cavity in the washing area and the position of the bottom of the accommodation cavity in the drying area.

[0037] As an implementation manner, the second functional component includes a fourth carrier member, a heating member, and a mixing member. The fourth carrier member is used to carry the nucleic acid extraction container to perform an incubation action. The heating member is used to heat the liquid containing at least the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member; the mixing member is used to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member.

[0038] As an implementation manner, the second functional component further includes a first driving member, and the first driving member is used to drive the fourth carrier member to move so that the fourth carrier member drives the nucleic acid extraction container to move;

[0039] The mixing member includes a plurality of magnets, and the plurality of magnets are alternately distributed on opposite sides in the horizontal direction of the movement track of the nucleic acid extraction container.

[0040] As an implementation manner, the mixing member at least includes a first magnet array and a second magnet array that extend horizontally. Each magnet array includes a plurality of magnets that are spaced apart and mounted on a magnet bracket.

[0041] The fourth bearing member includes a container receiving portion for placing the nucleic acid extraction container.

[0042] The fourth bearing member and the mixing member are arranged relative to each other such that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array.

[0043] The fourth bearing member and the mixing member can move horizontally relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion.

[0044] The fourth bearing member and the mixing member are further arranged relative to each other such that when the fourth bearing member and the mixing member move horizontally relative to each other, at least two of the magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

[0045] As an implementation manner, at least two of the magnets in the mixing member have different heights relative to the fourth bearing member; or,

[0046] The installation heights of at least two of the magnets in the mixing member on the magnet bracket are different, so that at least two of the magnets in the mixing member have different heights relative to the fourth bearing member.

[0047] As an implementation manner, the second functional component further includes a third driving member. The mixing member includes a movable magnet bracket and a plurality of magnets fixed on the magnet bracket. Among them, the third driving member is used to drive the magnet bracket to move, so as to drive the plurality of magnets fixed on the magnet bracket to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement in the liquid of the nucleic acid extraction container; or,

[0048] The second functional component further includes a third driving member. The mixing member is used to generate a magnetic field, and the third driving member is used to drive the fourth carrying member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth carrying member, so that the magnetic beads in the nucleic acid extraction container can perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or,

[0049] The second functional component further includes a third driving member. The fourth carrying member at least includes a lysis area. The nucleic acid extraction container undergoes a lysis and capture step in the lysis area. In this lysis and capture step, the cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by the magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field that at least covers the lysis area, and the third driving member is used to drive the fourth carrying member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis area move, so that the magnetic beads can capture the released nucleic acids.

[0050] As an implementation manner, the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is installed on the frame assembly;

[0051] The second functional component includes a fourth carrying member and a first driving member. The fourth carrying member is movably installed on the frame assembly to carry the nucleic acid extraction container to perform an incubation action, and the fourth carrying member is formed with at least one first transfer station and a plurality of accommodating cavities. Each accommodating cavity is used to accommodate one nucleic acid extraction container. The first driving member is used to drive the fourth carrying member to move relative to the frame assembly so that the fourth carrying member drives a plurality of nucleic acid extraction containers to move to the first transfer station respectively; the first carrying member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer station and the first carrying member.

[0052] As an implementation manner, the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is installed on the frame assembly;

[0053] The second functional component includes a fourth carrying member which is fixed to the frame assembly for carrying the nucleic acid extraction container to perform an incubation action, and at least one first transfer station and a plurality of accommodating cavities are formed on the fourth carrying member, and each accommodating cavity is used for accommodating one nucleic acid extraction container; the first carrying member is fixed to the frame assembly; the transfer component is at least used for transferring the nucleic acid extraction container between the first transfer station and the first carrying member, and the transfer component is configured to be capable of at least three-dimensional movement.

[0054] As an implementation manner, the sample analyzer further includes:

[0055] A container providing device for providing the nucleic acid extraction container and the amplification reaction container;

[0056] A reagent storage bin for storing a first type of reagent and a second type of reagent, where the first type of reagent is used to react with a sample to obtain a nucleic acid extraction solution, and the second type of reagent is used to react with the nucleic acid extraction solution for amplification;

[0057] A reagent dispensing device for dispensing the first type of reagent stored in the reagent storage bin into the nucleic acid extraction container, and for dispensing the second type of reagent stored in the reagent storage bin into the amplification reaction container;

[0058] A scheduling device for at least scheduling the nucleic acid extraction container provided by the container providing device to the nucleic acid extraction device and scheduling the amplification reaction container provided by the container providing device to the amplification device.

[0059] The second object of the present invention is to provide a sample analyzer, which includes:

[0060] A sample dispensing device for sucking a sample from a sample container and dispensing at least a part of the sucked sample into a nucleic acid extraction container;

[0061] A nucleic acid extraction device for performing nucleic acid extraction on the liquid in the nucleic acid extraction container containing at least a sample and a reagent to obtain a nucleic acid extraction solution;

[0062] A pipetting device for transferring the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container;

[0063] An amplification device for amplifying the nucleic acid extraction solution in the amplification reaction container to obtain a test solution;

[0064] Detection device, which is used to detect the liquid to be tested;

[0065] Among them, the nucleic acid extraction device includes a first functional component, a second functional component and a transfer component. The first functional component is arranged above or below the second functional component. The transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component. The first functional component is at least used to carry the nucleic acid extraction container to perform a first functional action, and the second functional component is at least used to carry the nucleic acid extraction container to perform a second functional action for nucleic acid extraction;

[0066] The first functional action and the second functional action are different functional actions or the same functional action executed successively.

[0067] As an implementation manner, the first functional action includes at least one of a liquid suction action, a liquid injection action, and a magnetic adsorption action; and / or,

[0068] The second functional action includes at least one of an incubation action and a mixing action.

[0069] As an implementation manner, the second functional component includes a fourth carrying member and a mixing member. The fourth carrying member is used to carry the nucleic acid extraction container to perform an incubation action, and the mixing member is used to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth carrying member;

[0070] Among them, the mixing member at least includes a first magnet array and a second magnet array extending horizontally. Each magnet array respectively includes a plurality of magnets spaced and installed on a magnet bracket;

[0071] The fourth carrying member includes a container receiving portion for placing the nucleic acid extraction container;

[0072] The fourth carrying member and the mixing member are arranged relative to each other such that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array;

[0073] The fourth carrying member and the mixing member can move horizontally relative to each other, so that the mixing member can mix at least the liquid containing the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion;

[0074] The fourth bearing member and the mixing member are also arranged relative to each other such that when the fourth bearing member and the mixing member move horizontally relative to each other, at least two of the magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

[0075] As an implementation manner, the second functional component includes a fourth bearing member, a mixing member, and a third driving member. The fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action. The mixing member is used to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth bearing member. The mixing member includes a movable magnet holder and a plurality of magnets fixed on the magnet holder. The third driving member is used to drive the magnet holder to move, so as to drive the plurality of magnets fixed on the magnet holder to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement under the action of the moving magnets; or,

[0076] The second functional component includes a fourth bearing member, a mixing member, and a third driving member. The fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action. The mixing member is used to generate a magnetic field to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth bearing member. The third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container can perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or,

[0077] The second functional component includes a fourth bearing member, a mixing member, and a third driving member. The fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action. The fourth bearing member at least includes a lysis area. The nucleic acid extraction container undergoes a lysis capture step in the lysis area. In this lysis capture step, the cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field at least covering the lysis area to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located in the lysis area. The third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis area move, so that the magnetic beads can capture the released nucleic acids.

[0078] The third object of the present invention is to provide a sample analyzer, which comprises:

[0079] A sample dispensing device for sucking a sample from a sample container and dispensing at least a part of the sucked sample into a nucleic acid extraction container;

[0080] A nucleic acid extraction device for performing nucleic acid extraction on a liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract;

[0081] A pipetting device for transferring the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0082] An amplification device for amplifying the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0083] A detection device for detecting the test solution;

[0084] Wherein, the nucleic acid extraction device has at least two workstations, each of which is respectively used to carry the nucleic acid extraction container to perform a processing action for nucleic acid extraction, and at least two of the workstations are distributed vertically up and down, and the processing action includes at least one of an incubation action, a mixing action, a magnetic adsorption action, a liquid suction action, and a liquid injection action.

[0085] As an implementation manner, the nucleic acid extraction device performing nucleic acid extraction on a liquid containing at least a sample and a reagent includes: a process of lysing the liquid containing at least a sample and a reagent and the subsequent processing after lysis.

[0086] As an implementation manner, the nucleic acid extraction device has a first-layer space and a second-layer space, and the first-layer space is arranged above or below the second-layer space;

[0087] An aspiration and injection liquid workstation, a single liquid suction workstation, and a single liquid injection workstation are arranged in the first-layer space;

[0088] A lysis incubation workstation, a washing incubation workstation, a drying incubation workstation, and an elution incubation workstation are arranged in the second-layer space;

[0089] Wherein, the lysis incubation workstation is used to carry the nucleic acid extraction container to perform a lysis incubation action;

[0090] The washing incubation workstation is used to carry the nucleic acid extraction container to perform a washing incubation action;

[0091] The drying incubation workstation is used to carry the nucleic acid extraction container to perform a drying incubation action;

[0092] The elution incubation station is used to hold the nucleic acid extraction container and perform elution incubation operations;

[0093] The liquid suction and injection station is used to hold the nucleic acid extraction container and perform a first liquid suction operation and a first liquid injection operation of injecting a cleaning solution;

[0094] The single liquid suction station is used to hold the nucleic acid extraction container and perform a second liquid suction operation;

[0095] The single liquid injection station is used to hold the nucleic acid extraction container and perform a second liquid injection operation of injecting an elution reagent.

[0096] The fourth object of the present invention is to provide a sample analyzer, which includes:

[0097] A sample dispensing device, which is used to aspirate a sample from a sample container and dispense at least part of the aspirated sample into a nucleic acid extraction container;

[0098] A nucleic acid extraction device, which is used to perform nucleic acid extraction operations on the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract;

[0099] A pipetting device, which is used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0100] An amplification device, which is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0101] A detection device, which is used to detect the test solution;

[0102] Wherein, the nucleic acid extraction device includes a lysis module, a cleaning module, an elution module and a transfer component. At least two of the lysis module, the cleaning module and the elution module are distributed vertically. The lysis module is used to hold the nucleic acid extraction container and perform lysis operations; the cleaning module is used to hold the nucleic acid extraction container and perform cleaning operations; the elution module is used to hold the nucleic acid extraction container and perform elution operations; the transfer component is used to sequentially transfer the nucleic acid extraction container to the lysis module, the cleaning module and the elution module.

[0103] As an implementation manner, the nucleic acid extraction by the nucleic acid extraction device on the liquid containing at least a sample and a reagent includes: a process of lysing the liquid containing at least a sample and a reagent and the subsequent treatment after lysis.

[0104] As an implementation manner, the nucleic acid extraction device further includes a drying module, which is arranged below or above at least one of the lysis module, the washing module, and the elution module. The drying module is used to carry the nucleic acid extraction container for drying operation, and the transfer component is used to sequentially transfer the nucleic acid extraction container to the lysis module, the washing module, the drying module, and the elution module.

[0105] For the sample analyzer provided by the first object of the present invention, by arranging the first functional component for at least magnetically adsorbing and sucking the liquid in the nucleic acid extraction container in the nucleic acid extraction device above or below the second functional component for at least incubating the liquid in the nucleic acid extraction container, and transferring the nucleic acid extraction container between the first functional component and the second functional component through the transfer component, that is: arranging the first functional component and the second functional component for performing different functional actions on the liquid in the nucleic acid extraction container or for sequentially performing the same functional action up and down in the vertical direction. When the liquid in the nucleic acid extraction container needs to perform an incubation action, the transfer component transfers the nucleic acid extraction container to the second functional component; when the liquid in the nucleic acid extraction container needs to perform magnetic adsorption and sucking actions, the transfer component transfers the nucleic acid extraction container to the first functional component. Adopting this setting scheme can make full use of the vertical space to arrange different functional components of the nucleic acid extraction device, thereby facilitating reducing the horizontal occupied space of the nucleic acid extraction device, further facilitating the miniaturized design of the sample analyzer, and facilitating improving the space utilization of the laboratory where the sample analyzer is located.

[0106] For the sample analyzer provided by the second object of the present invention, by arranging one of the first functional component and the second functional component for performing different functional actions or for sequentially performing the same functional action in the nucleic acid extraction device above or below the other, and transferring the nucleic acid extraction container between the first functional component and the second functional component through the transfer component, that is: arranging the first functional component and the second functional component for performing different functional actions on the liquid in the nucleic acid extraction container or for sequentially performing the same functional action up and down in the vertical direction, and transferring the nucleic acid extraction container to the first functional component and the second functional component respectively through the transfer component. Adopting this setting scheme can make full use of the vertical space to arrange different functional components of the nucleic acid extraction device, thereby facilitating reducing the horizontal occupied space of the nucleic acid extraction device, further facilitating the miniaturized design of the sample analyzer, and facilitating improving the space utilization of the laboratory where the sample analyzer is located.

[0107] The sample analyzer provided by the third object of the present invention, by arranging at least two stations in the nucleic acid extraction device in an up-and-down distribution along the vertical direction, and setting each station to be used for carrying a nucleic acid extraction container to perform at least one of the processing actions of incubation action, mixing action, magnetic adsorption action, liquid suction action, and liquid injection action, that is, arranging at least two stations for carrying a nucleic acid extraction container to perform processing actions in an up-and-down arrangement along the vertical direction. Adopting this setting scheme can make full use of the vertical space to arrange multiple stations of the nucleic acid extraction device, thereby facilitating the reduction of the horizontal occupied space of the nucleic acid extraction device, and further facilitating the miniaturization design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located.

[0108] The sample analyzer provided by the fourth object of the present invention, by arranging at least two of the lysis module, cleaning module, and elution module in the nucleic acid extraction device in an up-and-down arrangement along the vertical direction, that is, arranging the functional modules for carrying a nucleic acid extraction container to perform different functional actions in sequence in an up-and-down arrangement along the vertical direction. Adopting this setting scheme can make full use of the vertical space to arrange multiple functional modules of the nucleic acid extraction device, thereby facilitating the reduction of the horizontal occupied space of the nucleic acid extraction device, and further facilitating the miniaturization design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located. Brief Description of the Drawings

[0109] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on the structures shown in these drawings.

[0110] Figure 1 It is a schematic diagram of the composition of the sample analyzer provided by Embodiment 1 of the present invention;

[0111] Figure 2 It is a three-dimensional structure diagram of a nucleic acid extraction device from one perspective provided by Embodiment 1 of the present invention;

[0112] Figure 3 It is a three-dimensional structure diagram of a nucleic acid extraction device from another perspective provided by Embodiment 1 of the present invention;

[0113] Figure 4 It is a flow diagram of a nucleic acid extraction container moving to different stations in the nucleic acid extraction device provided by Embodiment 1 of the present invention. The dotted arrows in the figure are used to indicate the moving direction of the nucleic acid extraction container between multiple stations;

[0114] Figure 5It is a schematic distribution diagram of the first transfer station provided in the first embodiment of the present invention.

[0115] Figure 6 It is a top view schematic diagram of the second functional component provided in the first embodiment of the present invention;

[0116] Figure 7 It is a three-dimensional structure schematic diagram of one perspective of the first functional component provided in the first embodiment of the present invention;

[0117] Figure 8 It is a three-dimensional structure schematic diagram of another perspective of the first functional component provided in the first embodiment of the present invention;

[0118] Figure 9 It is a structure schematic diagram of the first module provided in the first embodiment of the present invention;

[0119] Figure 10 It is a structure schematic diagram of the third module provided in the first embodiment of the present invention;

[0120] Figure 11 It is Figure 10 a partial enlarged schematic diagram;

[0121] Figure 12 It is a structure schematic diagram of the fourth module provided in the first embodiment of the present invention;

[0122] Figure 13 It is a structure schematic diagram of the transfer component and the nucleic acid extraction container provided in the first embodiment of the present invention;

[0123] Figure 14 It is a structure schematic diagram of the first pipette tip and the second pipette tip provided in the first embodiment of the present invention;

[0124] Figure 15 It is a three-dimensional structure schematic diagram of the mixing member provided in the first embodiment of the present invention;

[0125] Figure 16 It is a top view distribution schematic diagram of the first magnet array and the second magnet array provided in the first embodiment of the present invention;

[0126] Figure 17 It is an unfolded front view schematic diagram of the first magnet array and the second magnet array provided in the first embodiment of the present invention;

[0127] Figure 18 It is an unfolded top view schematic diagram of the first magnet array and the second magnet array provided in the first embodiment of the present invention;

[0128] Figure 19 It is a schematic distribution diagram of the first transfer station provided in the second embodiment of the present invention.

[0129] Description of the attached reference numerals: 1. Sample analyzer; 100. Nucleic acid extraction device; 110. First functional component; 111. First carrier member; 112. Second carrier member; 113. Third carrier member; 114. Second pipetting member; 115. Second magnetic attraction member; 116. First lifting member; 117. Third pipetting member; 118. First pipetting member; 119. First magnetic attraction member; 1001. First connecting plate; 1002. Second connecting plate; 1003. Third connecting plate; 1004. Fourth connecting plate; 101. First module; 102. Second module; 103. Third module; 104. Fourth module; 120. Second functional component; 121. Fourth carrier member; 1211. Lysis area; 1212. Washing area; 1213. Drying area; 1214. Elution area; 1215. First transfer station; 122. First driving member; 130. Transfer component; 140. Mixing member; 141. First magnet array; 1411. First magnet; 142. Second magnet array; 1421. Second magnet; 143. Magnet support; 200. Sample dispensing device; 300. Amplification device; 400. Detection device; 500. Container providing device; 600. Controller; 700. Pipetting device; 800. Sample storage device; 900. Housing assembly; 901. Reagent storage bin; 902. Reagent dispensing device; 903. Scheduling device; 10. Nucleic acid extraction container; 20. First pipetting tip; 30. Second pipetting tip; R. Extension direction; H. Installation height. Detailed implementation manners

[0130] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts shall fall within the protection scope of the present invention.

[0131] In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the fact that those of ordinary skill in the art can implement them. When the combination of the technical solutions results in contradictions or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the protection scope required by the present invention.

[0132] This embodiment mainly optimizes the layout of the nucleic acid extraction device in the sample analyzer, makes full use of the vertical space for up-and-down layout, solves the problem that the nucleic acid extraction device occupies a large planar area in the sample analyzer, and further solves the problem of large overall floor area in the sample analyzer.

[0133] Embodiment 1:

[0134] AsFigures 1 to 18 As shown in Figures 1 to 18 , a sample analyzer 1 provided in Embodiment 1 of the present invention includes a sample dispensing device 200, a nucleic acid extraction device 100, an amplification device 300, and a detection device 400. The sample dispensing device 200 is used to dispense samples; the nucleic acid extraction device 100 is used to extract nucleic acids from a liquid containing at least samples to obtain a nucleic acid extract; the amplification device 300 is used to amplify a liquid containing at least the nucleic acid extract to obtain a test solution; the detection device 400 is used to detect the test solution. The nucleic acid extraction device 100 is mainly used to extract nucleic acids from samples to obtain purified nucleic acids. The amplification device 300 is configured to amplify the nucleic acids extracted by the nucleic acid extraction device to greatly increase the amount of nucleic acids in a short time. The sample analyzer 1 provided in this embodiment is mainly used to obtain a test solution by performing nucleic acid extraction and amplification on samples, and then detect the test solution to obtain the detection result of the samples.

[0135] As an implementation manner, the nucleic acid extraction device 100 extracts nucleic acids from biological samples stored in the nucleic acid extraction container 10 based on the magnetic bead method. The amplification device 300 is used to amplify the nucleic acids extracted by the nucleic acid extraction device 100 so as to greatly increase the amount of nucleic acids in a short time. The detection device 400 is used to detect the amplified nucleic acids.

[0136] As an implementation manner, the nucleic acid extraction device 100 includes a first functional component 110, a second functional component 120, and a transfer component 130. The first functional component 110 is disposed above or below the second functional component 120. The transfer component 130 is configured to transfer the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120 to perform nucleic acid extraction to obtain a nucleic acid extraction solution. The first functional component 110 is configured to at least perform magnetic adsorption and liquid suction actions on the liquid in the nucleic acid extraction container 10, and the second functional component 120 is at least configured to incubate the liquid in the nucleic acid extraction container 10. The first functional component 110 and the second functional component 120 are two different functional components of the nucleic acid extraction device 100. Among them, the first functional component 110 is mainly configured to perform magnetic adsorption and liquid suction actions on the liquid in the nucleic acid extraction container 10, and the second functional component 120 is mainly configured to incubate the liquid in the nucleic acid extraction container 10. In this implementation scheme, by arranging the first functional component 110 and the second functional component 120, which perform different functional actions on the liquid in the nucleic acid extraction container 10, in an up-and-down arrangement along the vertical direction, and transferring the nucleic acid extraction container 10 up and down between the first functional component 110 and the second functional component 120 by the transfer component 130. When the liquid in the nucleic acid extraction container 10 needs to perform an incubation action, the transfer component 130 transfers the nucleic acid extraction container 10 to the second functional component 120. When the liquid in the nucleic acid extraction container 10 needs to perform magnetic adsorption and liquid suction actions, the transfer component 130 transfers the nucleic acid extraction container 10 to the first functional component 110. Adopting this setting scheme can make full use of the vertical space to arrange different functional components of the nucleic acid extraction device 100, thereby facilitating the reduction of the horizontal occupied space of the nucleic acid extraction device 100, further facilitating the miniaturized design of the sample analyzer 1, and facilitating the improvement of the space utilization of the laboratory where the sample analyzer 1 is located.

[0137] As an implementation manner, the sample analyzer 1 further includes a pipetting device 700. The sample dispensing device 200 is configured to aspirate at least a part of the sample from the sample container and dispense it into the nucleic acid extraction container 10 provided by the second consumable supply device; the nucleic acid extraction device 100 is configured to perform nucleic acid extraction on the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 to obtain a nucleic acid extraction solution, and the reagent contains magnetic beads; the pipetting device 700 is configured to transfer the nucleic acid extraction solution in the nucleic acid extraction container 10 to the amplification reaction container; the amplification device 300 is configured to amplify the liquid containing at least the nucleic acid extraction solution in the amplification reaction container to obtain a test solution. The sample container, the nucleic acid extraction container 10, and the amplification reaction container are three different containers, and the pipetting device 700 is mainly used to transfer the nucleic acid extraction solution between the nucleic acid extraction container 10 and the amplification reaction container. Among them, the sample container is mainly used to load the sample collected from the patient, that is, the sample container is mainly used to provide a placement place for the sample. The nucleic acid extraction container 10 is mainly used to carry the sample for nucleic acid extraction, that is, the nucleic acid extraction container 10 is mainly used to provide a nucleic acid extraction place for the sample. The amplification reaction container is mainly used to carry the nucleic acid extraction solution for amplification reaction, that is, the amplification reaction container is mainly used to provide an amplification reaction place for the nucleic acid extraction solution. The nucleic acid extraction container 10 and the amplification reaction container are two different consumables of the sample analyzer 1. In this way, the nucleic acid extraction container 10 can be designed into a shape convenient for nucleic acid extraction according to requirements, and the amplification reaction container can be designed into a shape convenient for amplification reaction according to requirements.

[0138] As an implementation manner, the first functional component 110 includes a first carrying member 111, a first liquid transfer member 118, and a first magnetic attraction member 119. The first carrying member 111 is used to carry the nucleic acid extraction container 10. The first magnetic attraction member 119 is used to perform a first magnetic adsorption on the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the first carrying member 111 to adsorb the magnetic beads in the nucleic acid extraction container 10. The first liquid transfer member 118 is used to perform a first liquid suction action on the liquid in the nucleic acid extraction container 10 located on the first carrying member 111 and subjected to the first magnetic adsorption by the first magnetic attraction member 119. The transfer component 130 is used to transfer the nucleic acid extraction container 10 between the first carrying member 111 and the second functional component 120. The magnetic adsorption includes the first magnetic adsorption, and the liquid suction action includes the first liquid suction action. The first magnetic attraction member 119 includes at least one magnet. During the incubation process, the nucleic acid in the biological sample will combine with the nucleic acid to form a whole. The first magnetic attraction member 119 is mainly used to perform a first magnetic adsorption on the liquid in the nucleic acid extraction container 10 placed on the first carrying member 111. The first magnetic adsorption here will adsorb the combination of the magnetic beads and the nucleic acid. The first liquid transfer member 118 is mainly used to suck away and drain the waste liquid in the nucleic acid extraction container 10, that is: the first liquid transfer member 118 is mainly used to suck away the liquid in the nucleic acid extraction container 10 except for the magnetic beads and the combination of the magnetic beads and the nucleic acid and drain it as waste liquid, which is conducive to realizing the purification of nucleic acid.

[0139] As an implementation manner, the first functional component 110 further includes a liquid injection member, and the liquid injection member is used to perform a first liquid injection action of injecting a cleaning solution into the nucleic acid extraction container 10 located on the first carrying member 111. The liquid injection member is mainly used to add a cleaning solution into the nucleic acid extraction container 10 to facilitate the subsequent cleaning function.

[0140] Specifically, the liquid injection member is mainly used to perform a first liquid injection action of adding a cleaning solution to the nucleic acid extraction container 10 after the first liquid suction action is completed. The added cleaning solution is used to clean the liquid in the nucleic acid extraction container 10. The second functional component 120 is used to carry the nucleic acid extraction container 10 to perform an incubation action, including: the second functional component 120 is used to carry the nucleic acid extraction container 10 to perform a lysis incubation action and a washing incubation action. The transfer component 130 is used to transfer the nucleic acid extraction container 10 that has completed the lysis incubation action from the second functional component 120 to the first carrying member 111, and is used to transfer the nucleic acid extraction container 10 that has sequentially completed the first liquid suction action and the first liquid injection action from the first carrying member 111 to the second functional component 120 to perform a washing incubation action.

[0141] As an implementation manner, the number of the first bearing members 111 is at least two, and at least one liquid injection member and at least one first liquid transfer member 118 are correspondingly arranged for each first bearing member 111. The at least two first bearing members 111 form at least two workstations arranged in sequence. After a first magnetic adsorption, a first liquid suction action, and a first liquid injection action are completed on one first bearing member 111, the nucleic acid extraction container 10 will be first scheduled to the second functional component 120 for cleaning and incubation, and then scheduled to the next first bearing member 111 to perform the next first magnetic adsorption, first liquid suction action, and first liquid injection action. In this implementation scheme, the nucleic acid extraction device 100 performs at least two magnetic adsorptions, waste liquid suction, and cleaning liquid injection on the liquid in the nucleic acid extraction container 10, so that the liquid in the nucleic acid extraction container 10 is correspondingly subjected to at least two cleanings and magnetic separations. In this way, it is beneficial to wash out and discharge the non-nucleic acid components in the sample, thereby facilitating the purification degree of the nucleic acid. Of course, in specific applications, as an alternative implementation scheme, the number of the first bearing members 111 can also be only one.

[0142] As an implementation manner, the number of the liquid injection members and the number of the first liquid transfer members 118 are both the same as the number of the first bearing members 111, that is, one liquid injection member and one first liquid transfer member 118 are correspondingly arranged for each first bearing member 111. In this way, it is beneficial to enable multiple nucleic acid extraction containers 10 to work in parallel on the first functional component 110, and it is beneficial to reduce the movement span of the liquid injection members and the first liquid transfer members 118. Of course, in specific applications, as an alternative implementation scheme, two first bearing members 111 can also share the same liquid injection member and / or share the same first liquid transfer member 118.

[0143] As an implementation manner, the number of the first bearing members 111, the number of the liquid injection members, and the number of the first liquid transfer members 118 are all two. Of course, in specific applications, the number of the first bearing members 111, the number of the liquid injection members, and the number of the first liquid transfer members 118 can also be three or more.

[0144] As an implementation manner, the first functional component 110 further includes a second carrier member 112, a second pipetting member 114, and a second magnetic attraction member 115. The second carrier member 112 is used to carry the nucleic acid extraction container 10. The second magnetic attraction member 115 is used to perform a second magnetic adsorption on the liquid containing at least a cleaning solution in the nucleic acid extraction container 10 located on the second carrier member 112. The second pipetting member 114 is used to perform a second liquid suction action on the liquid in the nucleic acid extraction container 10 located on the second carrier member 112 and subjected to the second magnetic adsorption by the second magnetic attraction member 115. The transfer component 130 is further used to transfer the nucleic acid extraction container 10 between the second carrier member 112 and the second functional component 120. The magnetic adsorption further includes the second magnetic adsorption, and the liquid suction action further includes the second liquid suction action. The first carrier member 111 and the second carrier member 112 form two workstations arranged in sequence. Only after the nucleic acid extraction container 10 has completed the first magnetic adsorption, the first liquid suction action, and the first liquid injection action on all the first carrier members 111 in sequence will it be scheduled to the second carrier member 112 to perform the second magnetic adsorption and the second liquid suction action. The second pipetting member 114 is used to perform the second liquid suction action on the nucleic acid extraction container 10 located on the second carrier member 112 and having completed all the first liquid suction actions and the first liquid injection action in sequence.

[0145] Different from the workstation formed by the first carrier member 111: The workstation formed by the second carrier member 112 is only used to perform magnetic adsorption and suction and discharge of waste liquid, without performing liquid injection; while for the workstation formed by the first carrier member 111, after performing magnetic adsorption and suction and discharge of waste liquid, it is also necessary to perform the action of injecting a cleaning solution.

[0146] As an implementation manner, the sample analyzer 1 further includes a first lifting member 116; the first lifting member 116 is connected to the second magnetic attraction member 115, and the first lifting member 116 is used to drive the second magnetic attraction member 115 to move up and down relative to the nucleic acid extraction container 10. In this implementation scheme, when the second magnetic attraction member 115 performs the action of moving from top to bottom, the magnetic conjugate (the conjugate of magnetic beads and nucleic acid) in the nucleic acid extraction container 10 can be dragged downward, so that the magnetic conjugate aggregates at the bottom of the nucleic acid extraction container 10, which is beneficial to avoiding the adverse phenomenon that the magnetic conjugate is too dispersed and the elution reagent cannot completely submerge the magnetic conjugate, beneficial to ensuring the reliability of the subsequent elution operation, and beneficial to preventing the second pipetting member 114 from contacting the magnetic conjugate or taking away the magnetic conjugate when sucking liquid.

[0147] Specifically, the first lifting member 116 is configured to: drive the second magnetic attraction member 115 to move downward after the transfer assembly 130 places the nucleic acid extraction container 10 on the second bearing member 112 and before the second pipetting member 114 performs the second liquid suction action; the second functional assembly 120 is further configured to carry the nucleic acid extraction container 10 to perform a drying incubation action; the transfer assembly 130 is further configured to transfer the nucleic acid extraction container 10 that has completed the washing and incubation actions from the second functional assembly 120 to the second bearing member 112, and to transfer the nucleic acid extraction container 10 that has completed the second liquid suction action from the second bearing member 112 to the second functional assembly 120 to perform a drying incubation action.

[0148] As an implementation manner, the first functional assembly 110 further includes a third bearing member 113 and a third pipetting member 117. The third bearing member 113 is configured to carry the nucleic acid extraction container 10, and the third pipetting member 117 is configured to perform a second liquid injection action of injecting an elution reagent into the nucleic acid extraction container 10 located on the third bearing member 113; the transfer assembly 130 is further configured to transfer the nucleic acid extraction container 10 between the third bearing member 113 and the second functional assembly 120. The first bearing member 111, the second bearing member 112, and the third bearing member 113 form three workstations arranged in sequence. The nucleic acid extraction container 10 will be scheduled to the third bearing member 113 to perform the second liquid injection action only after the first magnetic adsorption, the first liquid suction action, and the first liquid injection action are completed on all the first bearing members 111, and after the second magnetic adsorption and the second liquid suction action are completed on the second bearing member 112. The workstation formed by the third bearing member 113 is only used to perform the liquid injection action of injecting an elution reagent, and does not need to perform magnetic adsorption and liquid waste suction and discharge actions.

[0149] Specifically, the third pipetting member 117 is configured to perform a second liquid injection action of injecting an elution reagent into the nucleic acid extraction container 10 located on the third bearing member 113 and that has completed the second liquid suction action; the second functional assembly 120 is further configured to carry the nucleic acid extraction container 10 to perform an elution incubation action; the transfer assembly 130 is further configured to transfer the nucleic acid extraction container 10 that has completed the drying incubation action from the second functional assembly 120 to the third bearing member 113, and to transfer the nucleic acid extraction container 10 that has completed the second liquid injection action from the third bearing member 113 to the second functional assembly 120 to perform an elution incubation action.

[0150] As an implementation manner, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged side by side in the horizontal direction, that is, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged in a row in the horizontal direction. In this way, it is beneficial to simplify the movement trajectory of the transfer assembly 130.

[0151] As an implementation manner, the second bearing member 112 is disposed between the first bearing member 111 and the third bearing member 113 along the horizontal direction.

[0152] As an implementation manner, two first bearing members 111 , a second bearing member 112 , and a third bearing member 113 are sequentially arranged side by side in the horizontal direction.

[0153] As an embodiment, the nucleic acid extraction container 10 is formed with a reaction well, and the reaction well is used to carry samples and reagents for nucleic acid extraction. The sample dispensing device 200 is used to draw samples from the sample container and dispense at least part of the drawn samples into the reaction well. The nucleic acid extraction device 100 is used to extract nucleic acids from the liquid containing at least samples and reagents in the reaction well. The liquid transfer device 700 is used to transfer the nucleic acid extraction liquid in the reaction well to the amplification reaction container.

[0154] As an embodiment, the nucleic acid extraction container 10 is also formed with at least one pipette tip hole, and each pipette tip hole is used to carry a pipette tip. The nucleic acid extraction device 100 is also used to perform pipetting through at least one pipette tip on the nucleic acid extraction container 10. After a pipette tip completes the corresponding pipetting action in a detection item, it is released to the recovery position together with the nucleic acid extraction container 10 for recovery, so as to save the cleaning process and improve the detection efficiency. In addition, the pipette tip is directly placed in the nucleic acid extraction container 10. On the one hand, it is not necessary to separately set up a storage bin and a scheduling mechanism for the pipette tip in the sample analyzer 1; on the other hand, it is beneficial to shorten the scheduling stroke of the pipette tip when using the pipette tip for pipetting, thereby improving the pipetting efficiency.

[0155] As an embodiment, the nucleic acid extraction container 10 is further formed with a first suction head hole, the first suction head hole is used to carry the first liquid transfer head 20, and the first liquid transfer member 118 is used to perform a first liquid transfer action on the liquid in the reaction hole through the first liquid transfer head 20. In this embodiment, the first liquid transfer member 118 uses the liquid transfer head to transfer waste liquid in the nucleic acid extraction process, which can omit the needle washing step of the first liquid transfer member 118, which is beneficial to improving the efficiency of nucleic acid extraction and avoiding the adverse phenomenon of cross contamination caused by incomplete cleaning.

[0156] As an embodiment, the first liquid transfer member 118 includes a first suction head connection component and a first suction and discharge power component; the first suction head connection component is used to be detachably connected to the first liquid transfer head 20, so as to suck liquid through the first liquid transfer head 20 under the action of the power provided by the first suction and discharge power component. The first suction head connection component is mainly used to be plugged and matched with the first liquid transfer head 20 to achieve clamping of the first liquid transfer head 20. The first suction and discharge power component is mainly used to provide driving force for the first liquid transfer head 20 to suck and discharge liquid.

[0157] As an implementation manner, the first pipetting member 118 further includes a first moving power component, which is used to drive the first pipette tip connecting component to move so that the first pipette tip connecting component moves to different work positions respectively, such as a standby position, a pipette tip clamping position, a liquid suction position, a liquid discharge position, a pipette tip releasing position, etc.

[0158] As an implementation manner, the first moving power component is used to drive the first pipette tip connecting component to move so that the first pipette tip connecting component moves above the first pipette tip hole, connects the first pipette tip connecting component with the first pipetting tip 20, and drives the first pipetting tip 20 to move and insert into the reaction hole by the first pipette tip connecting component; the first suction and discharge power component is used to provide a driving force for the first pipetting tip 20 to perform a liquid suction action after the first pipetting tip 20 is inserted into the reaction hole.

[0159] As an implementation manner, the nucleic acid extraction container 10 is further formed with a second pipette tip hole for carrying the second pipetting tip 30, and the third pipetting member 117 is used to perform a second liquid injection action into the reaction hole through the second pipetting tip 30. In this implementation scheme, the third pipetting member 117 sucks the waste liquid in the nucleic acid extraction process through the pipette tip, which can omit the needle washing step of the third pipetting member 117 and is beneficial to improving the efficiency of nucleic acid extraction.

[0160] As an implementation manner, the volume of the first pipetting tip 20 is larger than that of the second pipetting tip 30. The first pipetting tip 20 is used to perform suction and discharge of waste liquid, and the amount of liquid sucked is relatively large; the second pipetting tip 30 is used to suck and discharge the elution reagent, and the amount of liquid sucked is relatively small. Here, setting the volume of the first liquid suction pipette tip to be larger than that of the second pipetting tip 30 is beneficial to enabling the first liquid suction pipette tip to suck away the waste liquid in the reaction hole at one time, reducing the number of suction and discharge liquid actions of the first liquid suction pipette tip, thereby being beneficial to ensuring the efficiency of suction and discharge of waste liquid, and on the other hand, being beneficial to ensuring the accuracy of suction and injection of the elution reagent.

[0161] As an implementation manner, the third pipetting member 117 includes a second pipette tip connecting component and a second suction and discharge power component. The second pipette tip connecting component is used to be detachably connected to the second pipetting tip 30 to suck liquid through the second pipetting tip 30 under the action of the power provided by the second suction and discharge power component.

[0162] As an implementation manner, the third pipetting member 117 further includes a second moving power component, which is used to drive the second pipette tip connecting component to move so that the second pipette tip connecting component moves to different work positions respectively, such as a standby position, a pipette tip clamping position, a liquid suction position, a liquid discharge position, a pipette tip releasing position, etc.

[0163] Specifically, the second moving power component is used to drive the second suction head connecting component to move, so that the second suction head connecting component moves above the second suction head hole, connects the second suction head connecting component with the second pipetting tip 30, drives the second pipetting tip 30 by the second suction head connecting component to move to the elution reagent suction level, and drives the second pipetting tip 30 by the second suction head connecting component to move and insert into the reaction well; the second suction and discharge power component is used to provide driving force for the second pipetting tip 30 to perform a liquid suction action after the second pipetting tip 30 moves to the elution reagent suction level, and provide driving force for the second pipetting tip 30 to perform a second liquid injection action after the second pipetting tip 30 inserts into the reaction well.

[0164] As an implementation manner, the nucleic acid extraction container 10 is further formed with a waste liquid hole, and the first pipetting member 118 and the second pipetting member 114 are further used to discharge the liquid sucked from the reaction well to the waste liquid hole. In this implementation, directly forming the waste liquid hole on the nucleic acid extraction container 10 can facilitate shortening the path of waste liquid discharge during the nucleic acid extraction process.

[0165] As an implementation manner, the second pipetting member 114 includes a third suction head connecting component, a third suction and discharge power component, and a third moving power component. The third suction head connecting component is used to connect with the first pipetting tip 20. The third moving power component is used to drive the third suction head connecting component to move, so that the third suction head connecting component moves above the first suction head hole, connects the third suction head connecting component with the first pipetting tip 20, and drives the first pipetting tip 20 by the third suction head connecting component to move and insert into the reaction well; the third suction and discharge power component is used to provide driving force for the first pipetting tip 20 to perform a liquid suction action after the first pipetting tip 20 inserts into the reaction well.

[0166] As an implementation manner, the liquid injection member includes a liquid injection needle, a liquid injection pipeline, and a fourth suction and discharge power component. The liquid injection pipeline is used to connect the liquid injection needle with the cleaning liquid container. The fourth suction and discharge power component is used to drive the liquid injection pipeline to suck the cleaning liquid from the cleaning liquid container, transport it to the liquid injection needle, and inject it into the reaction well of the nucleic acid extraction container 10 located on the first bearing member 111 through the liquid injection needle.

[0167] As an implementation manner, the sample analyzer 1 further includes a frame assembly; the first functional assembly 110 includes a first connecting plate 1001, a second connecting plate 1002, a third connecting plate 1003, a fourth connecting plate 1004, two first carrying members 111, two first liquid transfer members 118, two liquid injection members, two first magnetic attraction members 119, a second carrying member 112, a second liquid transfer member 114, a second magnetic attraction member 115, a third carrying member 113, and a third liquid transfer member 117; the first connecting plate 1001 is connected to a first carrying member 111, a first liquid transfer member 118, a liquid injection member, and a first magnetic attraction member 119 to form a first module 101, and the first module 101 is connected to the frame assembly through the first connecting plate 1001; the second connecting plate 1002 is connected to another first carrying member 111, another first liquid transfer member 118, another liquid injection member, and another first magnetic attraction member 119 to form a second module 102, and the second module 102 is connected to the frame assembly through the second connecting plate 1002; the third connecting plate 1003 is connected to the second carrying member 112, the second liquid transfer member 114, and the second magnetic attraction member 115 to form a third module 103, and the third module 103 is connected to the frame assembly through the third connecting plate 1003; the fourth connecting plate 1004 is connected to the third carrying member 113 and the third liquid transfer member 117 to form a fourth module 104, and the fourth module 104 is connected to the frame assembly through the fourth connecting plate 1004.

[0168] As an implementation manner, the first module 101, the second module 102, the third module 103, and the fourth module 104 are arranged side by side in sequence along the horizontal direction.

[0169] As an implementation manner, the first module 101, the second module 102, the third module 103, and the fourth module 104 are arranged side by side in sequence along a linear trajectory.

[0170] As an implementation manner, the second functional assembly 120 includes a fourth carrying member 121 and a heating member. The fourth carrying member 121 is used to carry the nucleic acid extraction container 10 to perform an incubation action, and the heating member is used to heat the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the fourth carrying member 121. The heating member is mainly used to provide a suitable incubation environment temperature for the liquid in the nucleic acid extraction container 10, so as to facilitate ensuring the incubation efficiency.

[0171] As an implementation manner, the fourth carrier member 121 includes a lysis zone 1211, a washing zone 1212, a drying zone 1213, and an elution zone 1214. The lysis zone 1211, the washing zone 1212, the drying zone 1213, and the elution zone 1214 are respectively formed with accommodation cavities for placing the nucleic acid extraction container 10. The transfer assembly 130 is configured to transfer the nucleic acid extraction container 10 between the lysis zone 1211, the washing zone 1212 and the first carrier member 111, between the washing zone 1212, the drying zone 1213 and the second carrier member 112, and between the drying zone 1213, the elution zone 1214 and the third carrier member 113. Specifically, the transfer assembly 130 is configured to: transfer the nucleic acid extraction container 10 located in the lysis zone 1211 to the first carrier member 111, transfer the nucleic acid extraction container 10 located in the first carrier member 111 to the washing zone 1212, transfer the nucleic acid extraction container 10 located in the washing zone 1212 to the second carrier member 112, transfer the nucleic acid extraction container 10 located in the second carrier member 112 to the drying zone 1213, transfer the nucleic acid extraction container 10 located in the drying zone 1213 to the third carrier member 113, and transfer the nucleic acid extraction container 10 located in the third carrier member 113 to the elution zone 1214. In this implementation scheme, the nucleic acid extraction performed by the nucleic acid extraction device 100 mainly includes links such as lysis, washing, drying, and elution. The lysis link is performed in the lysis zone 1211. In the lysis link, the cells in the sample are mainly ruptured by a lysis reagent to release nucleic acids, and the released nucleic acids are adsorbed on the magnetic beads in the nucleic acid extraction container 10. The washing link is performed in the washing zone 1212. In the washing link, the unnecessary components in the sample are mainly removed by a cleaning solution (or called a washing solution), such as washing various impurities such as proteins and lipids remaining on and between the magnetic beads. The drying link is performed in the drying zone 1213. In the drying link, the washed liquid is mainly dried by heating. The elution link is performed in the elution zone 1214. In the elution link, the nucleic acids are mainly separated from the magnetic beads by an elution reagent, so that purified nucleic acids can be obtained. Of course, in specific applications, the steps of nucleic acid extraction are not limited to this. For example, as an alternative implementation scheme, it is also possible not to provide the drying zone 1213 and the drying link.

[0172] As an implementation manner, the fourth carrier member 121 is formed with two washing areas 1212, namely a first washing area 1212 and a second washing area 1212. The first functional component 110 includes two first carrier members 111. The transfer component 130 transfers the nucleic acid extraction container 10 located in the lysis area 1211 to the first carrier member 111, transfers the nucleic acid extraction container 10 located on the first carrier member 111 to the washing area 1212, and transfers the nucleic acid extraction container 10 located in the washing area 1212 to the second carrier member 112, including: transferring the nucleic acid extraction container 10 that has completed lysis incubation in the lysis area 1211 to one first carrier member 111, transferring the nucleic acid extraction container 10 that has completed the first liquid suction action and the first liquid injection action on this first carrier member 111 to the first washing area 1212, transferring the nucleic acid extraction container 10 that has completed washing incubation in the first washing area 1212 to the other first carrier member 111, transferring the nucleic acid extraction container 10 that has completed the first liquid suction action and the first liquid injection action on the other first carrier member 111 to the second washing area 1212, and transferring the nucleic acid extraction container 10 that has completed washing incubation in the second washing area 1212 to the second carrier member 112. In this implementation scheme, setting two washing areas 1212 is conducive to ensuring the cleaning degree of washing, and thus conducive to ensuring the purification degree of nucleic acid. Of course, in specific applications, the number of the washing areas 1212 is not limited to this. For example, it can also be one, three or even more.

[0173] As an implementation manner, the fourth carrier member 121 includes an incubation tray, and a heating member is used to heat the nucleic acid extraction container 10 on the incubation tray; the incubation tray is formed with a lysis area 1211, a washing area 1212, a drying area 1213 and an elution area 1214, and a first transfer station 1215 is formed on the incubation tray. The incubation tray is used to respectively schedule the nucleic acid extraction containers 10 located in the lysis area 1211, the washing area 1212, the drying area 1213 and the elution area 1214 to the first transfer station 1215, and the transfer component 130 is used to schedule the nucleic acid extraction container 10 located at the first transfer station 1215 to the first functional component 110. In this implementation scheme, the transfer component 130 only picks up and places the nucleic acid extraction container 10 on the fourth carrier member 121 at one transfer station (i.e., the first transfer station 1215), which is conducive to simplifying the movement track and structural complexity of the transfer component 130.

[0174] As an implementation manner, the fourth bearing member 121 is disc-shaped, and the fourth bearing member 121 is formed with a plurality of accommodating cavities arranged along the horizontal circumferential direction, and each accommodating cavity is used for accommodating a nucleic acid extraction container 10. The first functional component 110 includes a first bearing member 111, a second bearing member 112, a third bearing member 113, and at least three connecting plates. The at least three connecting plates are sequentially arranged at intervals. The first bearing member 111, the second bearing member 112, and the third bearing member 113 are respectively installed on one connecting plate. The first bearing member 111 is used for bearing the nucleic acid extraction container 10 to perform a first liquid suction action and a first liquid injection action of injecting a cleaning liquid. The second bearing member 112 is used for bearing the nucleic acid extraction container 10 to perform a second liquid suction action. The third bearing member 113 is used for bearing the nucleic acid extraction container 10 to perform a second liquid injection action of injecting an elution reagent.

[0175] As an implementation manner, the sample analyzer 1 further includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly; the fourth bearing member 121 is movably installed on the frame assembly to be used for bearing the nucleic acid extraction container 10 to perform an incubation action, and the fourth bearing member 121 is formed with at least one first transfer station 1215 and a plurality of accommodating cavities, and each accommodating cavity is used for accommodating a nucleic acid extraction container 10. The first driving member 122 is used for driving the fourth bearing member 121 to move relative to the frame assembly so that the fourth bearing member 121 drives a plurality of nucleic acid extraction containers 10 to move to the first transfer station 1215 respectively; the first bearing member 111 is fixed on the frame assembly; the transfer assembly 130 is at least used for transferring the nucleic acid extraction container 10 between the first transfer station 1215 and the first bearing member 111. The first pipetting member 118 is used for performing a first liquid suction action on the nucleic acid extraction container 10 located on the first bearing member 111. In this implementation scheme, the bearing members of the first functional component 110 are stationary relative to the frame assembly, and the bearing members of the second functional component 120 can move relative to the frame assembly.

[0176] As an implementation manner, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are installed on the frame assembly in a manner stationary relative to the frame assembly to carry the nucleic acid extraction container 10 and respectively perform the first liquid suction action, the second liquid suction action, and the second liquid injection action. That is, the second bearing member 112 and the third bearing member 113 are also installed on the frame assembly in a manner stationary relative to the frame assembly to carry the nucleic acid extraction container 10. The second pipetting member 114 is used to perform the second liquid suction action on the nucleic acid extraction container 10 located on the second bearing member 112 and having completed the first liquid suction action. The third pipetting member 117 is used to perform the second liquid injection action of injecting the elution reagent on the nucleic acid extraction container 10 located on the third bearing member 113 and having completed the second liquid suction action. The transfer assembly 130 is further used to: transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the second bearing member 112, and transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the third bearing member 113.

[0177] As an implementation manner, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged side by side in sequence along the horizontal straight line direction. The transfer assembly 130 includes a single first clamping member and a single second driving member. The first clamping member is used to clamp and release the nucleic acid extraction container 10. The second driving member is used to drive the first clamping member to perform three-dimensional linear motion so that the first clamping member moves to the first transfer station 1215, the first bearing member 111, the second bearing member 112, and the third bearing member 113 respectively to clamp and release the nucleic acid extraction container 10.

[0178] As an implementation manner, the sample analyzer 1 further includes a controller 600, and the controller 600 is configured to: control the transfer component 130 to schedule the nucleic acid extraction container 10 after the incubation action is performed in the lysis area 1211 to the first carrier member 111; control the first pipetting member 118 to perform a first liquid suction action on the liquid in the nucleic acid extraction container 10 on the first carrier member 111 and control the liquid injection member to perform a first liquid injection action on the nucleic acid extraction container 10 on the first carrier member 111; control the transfer component 130 to transfer the nucleic acid extraction container 10 after the first liquid injection action is performed to the washing area 1212 to perform an incubation action; control the transfer component 130 to transfer the nucleic acid extraction container 10 after the incubation action is performed in the washing area 1212 to the second carrier member 112; control the second pipetting member 114 to perform a second liquid suction action on the liquid in the nucleic acid extraction container 10 on the second carrier member 112; control the transfer component 130 to transfer the nucleic acid extraction container 10 after the second liquid suction action is performed to the drying area 1213 to perform a drying action on the nucleic acid extraction container 10; control the transfer component 130 to transfer the nucleic acid extraction container 10 after the drying action is performed in the drying area 1213 to the third carrier member 113; control the third pipetting member 117 to perform a second liquid injection action on the nucleic acid extraction container 10 on the third carrier member 113; control the transfer component 130 to transfer the nucleic acid extraction container 10 after the second liquid injection action is performed to the elution area 1214 to perform an elution action.

[0179] As an implementation manner, the nucleic acid extraction method includes the following steps: a lysis incubation step, a first aspiration and injection step, a first washing incubation step, a second aspiration and injection step, a second washing incubation step, a single aspiration step, a drying step, a dispensing elution reagent step, and an elution incubation step. Among them, the lysis incubation step mainly includes performing a lysis incubation step on the liquid in the nucleic acid extraction container 10 in the lysis area 1211. Specifically, the construction of the lysis system can be completed at other devices in the previous step. For example, a lysis reagent and a sample are added to the nucleic acid extraction container 10, and the nucleic acid extraction container 10 containing the lysis reagent and the sample is transferred to the accommodation cavity in the lysis area 1211, and the sample to be lysed in the lysis area 1211 is heated by a heating member. After the lysis incubation step is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform the first aspiration and injection step to assist the subsequent washing process. The first aspiration and injection step includes: magnetically adsorbing the magnetic conjugate in the nucleic acid extraction container 10. After the magnetic conjugate is adsorbed, the waste liquid in the nucleic acid extraction container 10 is aspirated and discharged, and a cleaning solution is injected after the waste liquid is aspirated. After the first aspiration and injection step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 to perform the following first washing incubation step: washing and incubating the liquid in the nucleic acid extraction container 10 in the first washing area 1212. After the first washing incubation step in the first washing area 1212 is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform the second aspiration and injection step. The second aspiration and injection step is similar to the first aspiration and injection step and will not be elaborated here. After the second aspiration and injection step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 to perform the second washing incubation step. The second washing incubation step is similar to the first washing incubation step and will not be elaborated here. After the second washing incubation step is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform the single aspiration step. The single aspiration step includes: magnetically adsorbing the magnetic conjugate in the nucleic acid extraction container 10. After the magnetic beads are adsorbed, the waste liquid in the nucleic acid extraction container 10 is aspirated and discharged. After the single aspiration step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 to perform the drying step. The drying step includes drying the liquid in the nucleic acid extraction container 10 in the drying area 1213. After the drying step is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform the dispensing elution reagent step. The dispensing elution reagent step includes: injecting an elution reagent into the reaction well of the nucleic acid extraction container 10. After the dispensing elution reagent step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 to perform the elution incubation step. The elution incubation step includes: performing an elution incubation step on the liquid in the nucleic acid extraction container 10 in the elution area 1214.

[0180]

[0180] As an implementation manner, the position of the bottom of the accommodating cavity in the elution area 1214 is higher than the position of the bottom of the accommodating cavity in the lysis area 1211, higher than the position of the bottom of the accommodating cavity in the washing area 1212, and higher than the position of the bottom of the accommodating cavity in the drying area 1213. Since the amount of liquid in the nucleic acid extraction container 10 during the lysis and washing steps is greater than the amount of liquid in the nucleic acid extraction container 10 during the elution step, in this implementation, the height position of the nucleic acid extraction container 10 in the elution area 1214 is set to be higher than the height positions in the lysis area 1211, the washing area 1212, and the drying area 1213, so that the magnetic conjugates in the nucleic acid extraction container 10 in the elution area 1214 can be concentrated at the bottom of the nucleic acid extraction container 10 as much as possible, avoiding nucleic acid loss.

[0181]

[0181] As an implementation manner, the position of the bottom of the accommodating cavity in the lysis area 1211 is at the same height as the position of the bottom of the accommodating cavity in the washing area 1212 and the position of the bottom of the accommodating cavity in the drying area 1213. The height position of the nucleic acid extraction container 10 in the lysis area 1211 is the same as the height positions in the washing area 1212 and the drying area 1213. Of course, in specific applications, the height positions of the nucleic acid extraction container 10 in at least two of the lysis area 1211, the washing area 1212, and the drying area 1213 can also be set to be different.

[0182]

[0182] As an implementation manner, the second functional component 120 further includes a first driving member 122, and the first driving member 122 is used to drive the fourth bearing member 121 to move so that the fourth bearing member 121 drives the nucleic acid extraction container 10 to move. The setting of the first driving member 122 can drive the nucleic acid extraction container 10 located in the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 to be respectively scheduled to the first transfer station 1215, so that the transfer component 130 transfers the nucleic acid extraction container 10 between the first transfer station 1215 and the first functional component 110.

[0183]

[0183] As an implementation manner, the second functional component 120 further includes a mixing member 140, and the mixing member 140 is used to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container 10 located on the fourth bearing member 121. In this way, it is beneficial to fully mix the sample and the reagent, thereby facilitating sufficient nucleic acid capture, washing, and elution.

[0184] As an implementation manner, the mixing member 140 includes a plurality of magnets, and the plurality of magnets are alternately distributed on opposite sides in the horizontal direction of the movement track of the nucleic acid extraction container 10. The magnets can generate a magnetic field, and the magnetic field can generate a magnetic force on the magnetic beads and magnetic conjugates in the nucleic acid extraction container 10. In this implementation, the magnets are used to mix the liquid in the nucleic acid extraction container 10, which can avoid direct contact between the mixing member 140 and the liquid in the nucleic acid extraction container 10, thereby avoiding contamination of the mixing member 140.

[0185] As an implementation manner, the mixing member 140 at least includes a first magnet array 141 and a second magnet array 142 that extend horizontally. Each magnet array includes a plurality of magnets spacedly mounted on a magnet bracket 143; the fourth bearing member 121 includes a container receiving portion for placing the nucleic acid extraction container 10; the container receiving portion is formed with a plurality of accommodation cavities for placing the nucleic acid extraction container 10. The first magnet array 141 includes a plurality of first magnets 1411 arranged in an array. The second magnet array 142 includes a plurality of second magnets 1421 distributed in an array.

[0186] As an implementation manner, the first magnet array 141 and the second magnet array 142 are parallel to each other in the horizontal extension direction R. The first magnet array 141 and the second magnet array 142 being parallel to each other in the horizontal extension direction R means that the horizontal extension directions R of the respective magnets in the first magnet array 141 and the second magnet array 142 are continuously distributed. In particular, the center connection lines of the respective magnets in the first magnet array 141 extend in the horizontal extension direction R and the center connection lines of the respective magnets in the second magnet array 142 extend in the horizontal extension direction R, while the magnets themselves can be arranged obliquely with respect to the horizontal extension direction R.

[0187] As an implementation manner, the fourth bearing member 121 and the mixing member 140 are arranged relative to each other such that when the nucleic acid extraction container 10 is received in the container receiving portion, at least the bottom of the nucleic acid extraction container 10 is located between the first magnet array 141 and the second magnet array 142, that is: the first magnet array 141 and the second magnet array 142 are distributed in the horizontal direction on opposite sides of the movement track of the nucleic acid extraction container 10. The fourth bearing member 121 and the mixing member 140 can move horizontally relative to each other, so that the mixing member 140 can mix the liquid containing at least the sample and magnetic beads in the nucleic acid extraction container 10 received in the container receiving portion.

[0188] As an implementation manner, the fourth bearing member 121 and the mixing member 140 are further arranged relative to each other such that when the fourth bearing member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0189] Since the fourth carrier member 121 and the mixing member 140 can move horizontally relative to each other, the fourth carrier member 121 can drive the nucleic acid extraction container 10 received in the container receiving portion and located between the first magnet array 141 and the second magnet array 142 to move horizontally relative to the first magnet array 141 and the second magnet array 142. Thus, a magnetic field varying in the horizontal direction is generated for the nucleic acid extraction container 10 received in the container receiving portion. In addition, when the fourth carrier member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 are at different heights from the bottom of the nucleic acid extraction container 10 received in the container receiving portion in the vertical direction. Therefore, a magnetic field varying in the vertical direction is also generated for the nucleic acid extraction container 10 received in the container receiving portion. By generating a magnetic field varying in the horizontal direction and in the vertical direction, the magnetic beads in the nucleic acid extraction container 10 placed in the varying magnetic field are spatially distributed as much as possible throughout the liquid in the nucleic acid extraction container 10 or the movement trajectory of the magnetic beads over time covers the entire liquid in the nucleic acid extraction container 10. In particular, the magnetic beads in the nucleic acid extraction container 10 placed in the varying magnetic field can perform three-dimensional movements (such as circumferential and axial movements), thereby achieving sufficient mixing of the magnetic beads.

[0190] When the nucleic acid extraction device 100 is in the working state, the relative horizontal movement of the fourth carrier member 121 and the mixing member 140 is uninterrupted and continuous.

[0191] It should be noted here that when it comes to the description of directions, the embodiments of the present invention are described based on the nucleic acid extraction device 100 being in the normal working state. In this normal working state, the nucleic acid extraction container 10 is vertically placed in the container receiving portion of the fourth carrier member 121, and the axial direction of the nucleic acid extraction container 10 is the vertical direction.

[0192] As an implementation manner, in order to achieve that at least two magnets have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion when the fourth carrier member 121 and the mixing member 140 move horizontally relative to each other, it can be stipulated that: when the fourth carrier member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 can move relative to the fourth carrier member 121 in the vertical direction. However, this implementation manner requires adding a mechanism for the relative vertical movement of the fourth carrier member 121 and the mixing member 140, which increases the complexity and cost of the sample analyzer 1.

[0193] As another simpler implementation, at least two magnets in the mixing member 140 have different heights relative to the fourth carrier member 121, that is, at least two magnets in the mixing member 140 are at different heights from the fourth carrier member 121. Preferably herein, there is no relative movement between the mixing member 140 and the fourth carrier member 121 in the vertical direction, especially there can be no relative movement in the vertical direction. Thus, it can be simply achieved that when the fourth carrier member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0194] As an implementation, the installation heights H of at least two magnets in the mixing member 140 on the magnet holder 143 are different, that is, at least two magnets in the mixing member 140 are fixed on the magnet holder 143 at different heights, so that at least two magnets in the mixing member 140 have different heights relative to the fourth carrier member 121.

[0195] It can be understood herein that the installation height H can be the vertical distance between the bottom surface of the magnet and the bottom surface of the magnet holder 143 when the magnet is installed on the magnet holder 143.

[0196] As an implementation, the installation heights H of at least two magnets in the first magnet array 141 on the magnet holder 143 are different. Alternatively or additionally, the installation heights H of at least two magnets in the second magnet array 142 on the magnet holder 143 are different.

[0197] As an implementation, the magnet holder 143 can be an integrally manufactured holder, and the magnets in the first magnet array 141 and the magnets in the second magnet array 142 are both installed on this integrally manufactured holder.

[0198] As an implementation, the magnet holder 143 can include a first holder and a second holder. The magnets in the first magnet array 141 are installed on the first holder, while the magnets in the second magnet array 142 are installed on the second holder.

[0199] As an implementation, the first magnet array 141 includes at least one first magnet 1411 unit and at least one second magnet 1421 unit. Each magnet unit includes one magnet or a plurality of continuously distributed magnets. Each magnet in the first magnet 1411 unit (also called the first lower layer magnet) is installed on the magnet holder 143 at a first height, and each magnet in the second magnet 1421 unit (also called the first upper layer magnet) is installed on the magnet holder 143 at a second height greater than the first height. That is to say, the first magnet array 141 includes at least two layers of magnets at different heights.

[0200] As an implementation manner, the first magnet array 141 includes two first magnet 1411 units and one second magnet 1421 unit, and each magnet unit respectively includes two magnets. Of course, in specific applications, the setting manner of the first magnet 1411 is not limited to this.

[0201] As an implementation manner, the second magnet array 142 includes at least one third magnet unit and at least one fourth magnet unit, and each magnet unit respectively includes one magnet or a plurality of continuously distributed magnets. Each magnet in the third magnet unit (also referred to as the second lower-layer magnet) is mounted on the magnet bracket 143 at a third height, and each magnet in the fourth magnet unit (also referred to as the second upper-layer magnet) is mounted on the magnet bracket 143 at a fourth height greater than the third height. That is to say, the second magnet array 142 includes at least two layers of magnets at different heights.

[0202] As an implementation manner, the first magnet array 141 includes two first magnet 1411 units and one second magnet 1421 unit, and each magnet unit respectively includes two magnets. The second magnet array 142 includes two third magnet units and one fourth magnet unit, and each magnet unit respectively includes two magnets.

[0203] As an implementation manner, the first height is equal to the third height. Alternatively or additionally, the second height is equal to the fourth height.

[0204] As an implementation manner, the first height may not be equal to the third height either, and the second height may not be equal to the fourth height either.

[0205] As an implementation manner, the first magnet 1411 units and the second magnet 1421 units are alternately arranged, especially arranged alternately in sequence, along the horizontal extension direction R to form the first magnet array 141. In this implementation scheme, the first magnet array 141 includes two layers of magnets at different heights.

[0206] As an implementation manner, the third magnet units and the fourth magnet units are alternately arranged, especially arranged alternately in sequence, along the horizontal extension direction R to form the second magnet array 142. In this implementation scheme, the second magnet array 142 includes two layers of magnets at different heights.

[0207] As an implementation manner, the first magnet array 141 may further include at least one fifth magnet unit, and the fifth magnet unit includes one magnet or a plurality of continuously distributed magnets. Each magnet in the fifth magnet unit is mounted on the magnet bracket 143 at a fifth height greater than the second height. Optionally, the second magnet array 142 may further include at least one sixth magnet unit, and the sixth magnet unit includes one magnet or a plurality of continuously distributed magnets. Each magnet in the sixth magnet unit is mounted on the magnet bracket 143 at a sixth height greater than the fourth height.

[0208] As an implementation manner, the fifth height is equal to the sixth height. In other embodiments, the fifth height may also not be equal to the sixth height.

[0209] As an implementation manner, the first magnet 1411 unit, the second magnet 1421 unit, and the fifth magnet unit are alternately arranged, especially arranged alternately in sequence, along the horizontal extension direction R to form the first magnet array 141. In this implementation scheme, the first magnet array 141 includes three layers of magnets at different heights.

[0210] As an implementation manner, the third magnet unit, the fourth magnet unit, and the sixth magnet unit are alternately arranged, especially arranged alternately in sequence, along the horizontal extension direction R to form the second magnet array 142. In this implementation scheme, the second magnet array 142 includes three layers of magnets at different heights.

[0211] In the above solution, the magnet units at different heights are alternately arranged to form the first magnet array 141 and the second magnet array 142 respectively. When the fourth bearing member 121 and the mixing member 140 move horizontally relative to each other, the mixing member 140 can generate a magnetic field that changes uniformly alternately in the horizontal direction and in the vertical direction, so that the magnetic beads in the nucleic acid extraction container 10 placed in the uniformly alternately changing magnetic field are distributed as uniformly as possible in the entire liquid of the nucleic acid extraction container 10 in space or the movement trajectories of the magnetic beads are uniformly spread over the entire liquid of the nucleic acid extraction container 10 in time. In particular, the magnetic beads in the nucleic acid extraction container 10 placed in the changing magnetic field can move uniformly along the circumferential and axial directions, thereby realizing the uniform and sufficient mixing of the magnetic beads.

[0212] As an implementation manner, each magnet unit includes two magnets, but the present invention is not limited thereto. For example, each magnet unit may include only one magnet, or may also include three, four or more magnets, and no specific limitation is made thereto.

[0213] As an implementation manner, the magnet units at different heights may also have different numbers of magnets, and no specific limitation is made thereto.

[0214] As an implementation manner, the extension direction R of the first magnet array 141 may be the circumferential direction or the linear direction.

[0215] As an implementation manner, the number of magnets in the first magnet array 141 and the second magnet array 142 may be equal or unequal.

[0216] As an implementation manner, the magnets in the first magnet array 141 and the second magnet array 142 are all magnets with the same specifications. It should be noted that the magnets in the first magnet array 141 and the second magnet array 142 can also be configured as magnets with different specifications according to requirements, such as different magnetic field intensities, different numbers, different sizes, etc., so as to adapt to the mixing in different scenarios.

[0217] As an implementation manner, the adjacent magnets of the first magnet array 141 and the second magnet array 142 are staggered along the horizontal extension direction R. That is to say, the connection line of the centers of the adjacent magnets of the first magnet array 141 and the second magnet array 142 is not perpendicular to the extension direction R. By staggering the magnets of the first magnet array 141 and the second magnet array 142 in the extension direction R, the following effects are achieved: when the nucleic acid extraction container 10 approaches the magnet, the magnetic field intensity and gradient generated at this place reach the maximum, and at this time, the magnetic beads can be quickly pulled to move, disturbing the fluid and promoting the spatial material exchange of the fluid; when the nucleic acid extraction container 10 is in the middle of the adjacent magnets, the magnetic field gradient at this place drops sharply, the magnetic beads are weakly stressed, and the magnetic beads are quickly dispersed in the spatial flow field under the influence of the flow field movement, realizing the full contact between the magnetic beads and the nucleic acid in the fluid.

[0218] As an implementation manner, the magnets in the first magnet array 141 are spaced apart by the same distance in the horizontal extension direction R. That is to say, the adjacent magnets in the first magnet array 141 have the same distance in the horizontal extension direction R.

[0219] Alternatively or additionally, the magnets in the second magnet array 142 are spaced apart by the same distance in the horizontal extension direction R. That is to say, the adjacent magnets in the second magnet array 142 have the same distance in the horizontal extension direction R.

[0220] As an implementation manner, the magnets in the first magnet array 141 and the magnets in the second magnet array 142 are both distributed along the circumferential direction as the extension direction R, and the first magnet array 141 and the second magnet array 142 are concentrically arranged. Specifically, a plurality of first magnets 1411 are distributed in a circular ring array, a plurality of second magnets 1421 are distributed in a circular ring array, and a plurality of second magnets 1421 are distributed on the periphery of a plurality of first magnets 1411. In this embodiment, the extension direction R of the magnet is the circumferential direction. In other embodiments, the extension direction R of the magnet is the linear direction, and the magnets in the first magnet array 141 and the magnets in the second magnet array 142 are both distributed along the linear direction as the extension direction R.

[0221] As an implementation manner, the magnet support 143 and the fourth bearing member 121 can be configured as discs, and at this time, each magnet array is a circular array. In another example, the magnet support 143 and the fourth bearing member 121 can be configured as sector discs, and at this time, each magnet array is an arc array.

[0222] As an implementation manner, the magnets in the first magnet array 141 have a first magnetic pole facing the fourth bearing member 121, the magnets in the second magnet array 142 have a second magnetic pole facing the fourth bearing member 121, and the polarities of the first magnetic pole and the second magnetic pole are opposite.

[0223] In other embodiments, the same-side magnetic poles of adjacent magnets in the first magnet array 141 have opposite polarities, and / or the same-side magnetic poles of adjacent magnets in the second magnet array 142 have opposite polarities.

[0224] Next, some implementation manners of driving the mixing member 140 to move horizontally relative to the fourth bearing member 121 are described.

[0225] As an implementation manner, the second functional component 120 further includes a third driving member. The mixing member 140 includes a movable magnet support 143 and a plurality of magnets fixed on the magnet support 143. Among them, the third driving member is used to drive the magnet support 143 to move, so as to drive the plurality of magnets fixed on the magnet support 143 to move relative to the nucleic acid extraction container 10 on the fourth bearing member 121.

[0226] As an implementation manner, the third driving member is configured to drive the magnet support 143 to move in the extending direction R, so that the mixing member 140 moves relative to the fourth bearing member 121 in the extending direction R, thereby driving the magnetic beads in the nucleic acid extraction container 10 located between the first magnet array 141 and the second magnet array 142 to move.

[0227] Specifically, when the third driving member drives the magnet support 143 to move, the magnetic beads in the nucleic acid extraction container 10 can move in a circular and axial cycle along the circumferential direction of the nucleic acid extraction container 10.

[0228] For example, when the third driving member drives the magnet support 143 to move in the extending direction R, the nucleic acid extraction container 10 placed on the fourth bearing member 121 can pass through each magnet of the first magnet array 141 and the second magnet array 142 at least once, preferably multiple times, in the extending direction R, so that the nucleic acid extraction container 10 is placed in a changing, preferably alternating, magnetic field.

[0229] For another example, when the third driving member drives the magnet holder 143 to move in the extending direction R, the nucleic acid extraction container 10 placed on the fourth bearing member 121 passes back and forth through at least a part of the magnets of the first magnet array 141 and the second magnet array 142 in the extending direction R, so that the nucleic acid extraction container 10 is placed in a changing, preferably alternately changing magnetic field.

[0230] As an implementation manner, the fourth bearing member 121 is configured as a rotatable first disc, the magnet holder 143 is configured as a rotatable second disc, and the first disc and the second disc are concentrically arranged.

[0231] Further preferably, the second disc is configured to be able to rotate synchronously with the first disc and to be able to rotate independently of the first disc. Specifically, the first disc can drive the second disc to rotate together, so that the rotation of the first disc does not affect the relative horizontal movement between the first disc and the second disc caused by the independent rotation of the second disc. That is to say, the rotating shafts of the second disc and the first disc are connected. When the first disc rotates, it can drive the second disc to rotate synchronously, and the second disc can also rotate independently, so that when the position of the nucleic acid extraction container 10 changes due to the rotation of the first disc, the relative movement relationship between the nucleic acid extraction container 10 and the magnetic field can basically remain unchanged.

[0232] As an implementation manner, the mixing member 140 includes a movable magnet holder 143 and a plurality of magnets fixed on the magnet holder 143. Among them, the third driving member is used to drive the magnet holder 143 to move, so as to drive the plurality of magnets fixed on the magnet holder 143 to move relative to the nucleic acid extraction container 10 on the fourth bearing member 121, so that the magnetic beads in the nucleic acid extraction container 10 placed on the fourth bearing member 121 perform three-dimensional movement in the liquid of the nucleic acid extraction container 10 under the action of the moving magnets.

[0233] As an implementation manner, the mixing member 140 is used to generate a magnetic field, and the third driving member is used to drive the fourth bearing member 121 and the mixing member 140 to move relative to each other, so that the mixing member 140 generates an alternately changing magnetic field relative to the nucleic acid extraction container 10 on the fourth bearing member 121, so that the magnetic beads in the nucleic acid extraction container 10 can perform three-dimensional movement in the liquid of the nucleic acid extraction container 10 under the action of the alternately changing magnetic field.

[0234] As an implementation manner, the fourth carrier member 121 at least includes a lysis region 1211. The nucleic acid extraction container 10 undergoes a lysis and capture process in the lysis region 1211. In this lysis and capture process, the cells in the sample of the nucleic acid extraction container 10 are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container 10. The mixing member 140 is used to generate a magnetic field that at least covers the lysis region 1211, and the third driving member is used to drive the relative movement of the fourth carrier member 121 and the mixing member 140, so that the magnetic beads in the nucleic acid extraction container 10 in the lysis region 1211 move, so as to capture the released nucleic acids by the magnetic beads.

[0235] As an implementation manner, the nucleic acid extraction device 100 is divided into an upper part and a lower part. The upper part of the nucleic acid extraction device 100 is mainly used to implement functions such as injection of cleaning liquid, magnetic bead separation, waste liquid suction and discharge, and injection of elution reagent. The lower part of the nucleic acid extraction device 100 is divided into five regions: a lysis region 1211, a first washing region 1212, a second washing region 1212, a drying region 1213, and an elution region 1214, which respectively implement functions such as lysis, washing, drying, and elution; the nucleic acid extraction container 10 can be clamped and transferred up and down by the transfer assembly 130 and placed at different workstations respectively to perform different functional actions.

[0236] As an implementation manner, the transfer assembly 130 is a manipulator. The functional components in the upper part of the nucleic acid extraction device 100 are divided into different workstations to implement corresponding functions. The lower part of the nucleic acid extraction device 100 is divided into different regions, and each region completes different functions, and then cooperates with the manipulator to clamp and transfer the nucleic acid extraction container 10.

[0237] As an implementation manner, the upper part of the nucleic acid extraction device 100 is provided with four workstations. Among them, workstation one is used for: performing operations of adsorbing magnetic beads, sucking away waste liquid, and injecting cleaning liquid on the lysed sample in the nucleic acid extraction container 10. Workstation two is used for: performing operations of sucking away waste liquid and injecting cleaning liquid on the sample in the nucleic acid extraction container 10 that has been cleaned and incubated in the first washing region 1212. Workstation three is used for: performing an operation of sucking away waste liquid on the sample in the nucleic acid extraction container 10 that has been cleaned and incubated in the second washing region 1212. Workstation four is used for: performing an operation of injecting elution reagent on the sample in the nucleic acid extraction container 10 that has been dried and incubated in the drying region 1213.

[0238] As an implementation, the transfer component 130 is used to: pick up the nucleic acid extraction container 10 from the second functional component 120 (i.e., the mixing and incubation module) below and place it at the four workstations of the first functional component 110 above, and pick up and place it from the four workstations of the first functional component 110 above to work at the second functional component 120 below. In an alternative embodiment, the transfer component 130 can also be used to transfer the nucleic acid extraction container 10 between different workstations of the first functional component 110 above and / or between different regions of the second functional component 120 below.

[0239] As an implementation, the amplification device 300 is mainly used to amplify the nucleic acid obtained by the nucleic acid extraction device 100, so as to greatly increase the amount of nucleic acid in a short time. Among them, the methods that can be used for amplification treatment include Polymerase Chain Reaction (PCR) method, Loop mediated isothermal amplification (LAMP) method, Isothermal chimeric primer initiated amplification (ICAN) method, Nuclear acid sequence-based amplification (NASBA) method, Strand displacement amplification (SDA) method, Ligase chain reaction (LCR) method, Rolling Circle Amplification (RCA) method.

[0240] As an implementation, the detection device 400 is mainly used to detect the amplified nucleic acid. For example, the detection device 400 is used to perform fluorescence detection on the amplified nucleic acid. During the nucleic acid amplification and real-time fluorescence detection processes, the signals of each nucleic acid amplification cycle are read to obtain a fluorescence amplification curve graph. The detection device 400 obtains a qualitative result of negative or positive according to the fluorescence amplification curve graph, or obtains a quantitative analysis result and the concentration of the measured substance in the sample to be tested based on the calibration curve.

[0241] As an implementation manner, the sample analyzer 1 further includes a container providing device 500, which is used to provide a nucleic acid extraction container 10 and an amplification reaction container. The sample container, the nucleic acid extraction container 10, and the amplification reaction container are three different containers. Among them, the sample container is mainly used to load the sample collected from the patient, that is, the sample container is mainly used to provide a placement place for the sample. The nucleic acid extraction container 10 is mainly used to carry the sample for nucleic acid extraction, that is, the nucleic acid extraction container 10 is mainly used to provide a nucleic acid extraction place for the sample. The amplification reaction container is mainly used to carry the nucleic acid extraction solution for amplification reaction, that is, the amplification reaction container is mainly used to provide an amplification reaction place for the nucleic acid extraction solution. The pipetting device 700 is mainly used to transfer the nucleic acid extraction solution between the nucleic acid extraction container 10 and the amplification reaction container. The nucleic acid extraction container 10 and the amplification reaction container are two different consumables of the sample analyzer 1. In this way, the nucleic acid extraction container 10 can be designed into a shape convenient for nucleic acid extraction according to requirements, and the amplification reaction container can be designed into a shape convenient for amplification reaction according to requirements. The nucleic acid extraction container 10 and the amplification reaction container are used as consumables. Specifically, the nucleic acid extraction container 10 and the amplification reaction container are both disposable containers. After a nucleic acid extraction container 10 completes the nucleic acid extraction of a detection project, it is discarded and recycled. After an amplification reaction container completes the amplification reaction and detection of a detection project, it is discarded and recycled, and there is no need to clean and reuse it. In this way, the container cleaning step in the detection process can be omitted, which is beneficial to improving the detection efficiency and avoiding the problem of cross-contamination affecting the accuracy of the sample detection result due to unclean cleaning. Each sample is detected by the sample analyzer 1, and at least one nucleic acid extraction container 10 and at least one amplification reaction container are consumed. In this implementation scheme, the nucleic acid extraction container 10 is provided by the second consumable supply device, and the amplification reaction container is provided by the first consumable supply device. In this way, the continuous detection requirements of batch samples can be met, and the situation that the sample detection efficiency is affected due to the lack of amplification nucleic acid extraction containers 10 and reaction containers can be avoided.

[0242] As an implementation manner, the container providing device 500 includes a first consumable supply device and a second consumable supply device. The first consumable supply device is at least used to provide the amplification reaction container. The second consumable supply device is at least used to provide the nucleic acid extraction container 10. The sample dispensing device 200 is used to aspirate at least part of the sample from the sample container and dispense it into the nucleic acid extraction container 10 provided by the second consumable supply device; the pipetting device 700 is used to aspirate at least part of the nucleic acid extraction solution from the nucleic acid extraction container 10 and dispense it into the amplification reaction container provided by the first consumable supply device.

[0243] As an implementation manner, the first consumable supply device is used to store the amplification reaction containers, that is, the first consumable supply device can buffer a certain amount of amplification reaction containers to meet the supply requirements of batch amplification reaction containers.

[0244] As an implementation manner, the first consumable supply device is also used for an operator or an operating robot to place an amplification reaction container to achieve the loading of the amplification reaction container, that is, the storage position and the loading position of the amplification reaction container in the sample analyzer 1 are at the same position. Of course, in specific applications, as an alternative implementation manner, the storage position and the loading position of the amplification reaction container in the sample analyzer 1 can also be at two different positions.

[0245] As an implementation manner, the second consumable supply device is used to store the nucleic acid extraction containers 10, that is, the second consumable supply device can buffer a certain amount of nucleic acid extraction containers 10 to meet the supply requirements of a batch of nucleic acid extraction containers 10.

[0246] As an implementation manner, the second consumable supply device is also used for an operator or an operating robot to place the nucleic acid extraction containers 10 to achieve the loading of the nucleic acid extraction containers 10, that is, the storage position and the loading position of the nucleic acid extraction containers 10 in the sample analyzer 1 are at the same position. Of course, in specific applications, as an alternative implementation manner, the storage position and the loading position of the nucleic acid extraction containers 10 in the sample analyzer 1 can also be at two different positions.

[0247] As an implementation manner, the second consumable supply device is arranged below or above the first consumable supply device, that is, the first consumable supply device and the second consumable supply device are arranged in the vertical direction, and the first consumable supply device and the second consumable supply device are distributed in two different upper and lower layers of space. This is conducive to arranging the consumable supply device with a large occupied space in one layer of space, so as to not only ensure that the sample analyzer 1 can cache a large number of consumables, but also reduce the size of the sample analyzer 1 in the horizontal direction, thereby effectively reducing the occupied space of the sample analyzer 1 and improving the space utilization rate of the laboratory where the sample analyzer 1 is located. Of course, in specific applications, as an alternative implementation manner, the first consumable supply device and the second consumable supply device can also be distributed in the horizontal direction.

[0248] As an implementation manner, the sample analyzer 1 further includes a scheduling device 903. The scheduling device 903 is at least used to schedule the nucleic acid extraction containers 10 provided by the container providing device 500 to the nucleic acid extraction device 100 and to schedule the amplification reaction containers provided by the container providing device 500 to the amplification device 300.

[0249] As an implementation manner, the scheduling device 903 includes a first scheduling mechanism and a second scheduling mechanism. The first scheduling mechanism is used to schedule the nucleic acid extraction containers 10 provided by the second consumable supply device to the nucleic acid extraction device 100, and the second scheduling mechanism is used to schedule the amplification reaction containers provided by the first consumable supply device to the amplification device 300.

[0250] As an implementation manner, the sample analyzer 1 further includes a sample storage device 800, and the sample storage device 800 is at least used for storing sample containers loaded with samples. The sample dispensing device 200 is used to aspirate at least part of the sample from the sample containers provided by the sample storage device 800 and dispense it into the nucleic acid extraction container 10 provided by the second consumable supply device. The sample storage device 800 can store a certain amount of sample containers to meet the continuous detection requirements of batch samples.

[0251] As an implementation manner, the sample storage device 800 is also used for an operator or an operating robot to place the sample container to achieve the loading of the sample container, that is, the storage position and the loading position of the sample container in the sample analyzer 1 are at the same position. Of course, in specific applications, as an alternative implementation, the storage position and the loading position of the sample container in the sample analyzer 1 can also be at two different positions.

[0252] As an implementation manner, the sample container is provided with an identification code, and the identification code is at least associated with the detection item information of the sample in the sample container. The sample analyzer 1 further includes an information acquisition component, and the information acquisition component is used to identify the identification code to at least acquire the detection item information of the sample.

[0253] As an implementation manner, the sample analyzer 1 further includes a reagent storage bin 901 and a reagent dispensing device 902. The reagent storage bin 901 is used to store reagents, and the reagent dispensing device 902 is used to dispense the reagents provided by the reagent storage device into the nucleic acid extraction container 10 and / or the amplification reaction container. In this implementation, the reagent dispensing device 902 is used to dispense the reagents into the nucleic acid extraction container 10 and / or the amplification reaction container; of course, in specific applications, as an alternative implementation, the reagents can also be pre-stored in the nucleic acid extraction container 10 and / or the amplification reaction container. In this way, the sample analyzer 1 may not be provided with the reagent storage bin 901 and the reagent dispensing device 902.

[0254] As an implementation manner, the reagent storage bin 901 is used to store a first type of reagent and a second type of reagent. The first type of reagent is used to react with the sample to obtain a nucleic acid extraction solution, and the second type of reagent is used to react with the nucleic acid extraction solution to perform amplification. The reagent dispensing device 902 is used to dispense the first type of reagent stored in the reagent storage bin 901 into the nucleic acid extraction container 10, and is used to dispense the second type of reagent stored in the reagent storage bin 901 into the amplification reaction container. The first type of reagent is mainly used for nucleic acid extraction, and the first type of reagent is also called an extraction reagent. The second type of reagent is mainly used for amplification reaction, and the second type of reagent is also called an amplification reagent.

[0255] As an implementation manner, the first type of reagent includes a lysis reagent, a magnetic bead reagent, and an elution reagent.

[0256] As an implementation manner, the reagent storage bin 901 is also used for an operator or an operating robot to place reagents for reagent loading, that is, the storage position and the loading position of the reagents in the sample analyzer 1 are at the same position. Of course, in specific applications, as an alternative implementation manner, the storage position and the loading position of the reagents in the sample analyzer 1 may also be at two different positions.

[0257] As an implementation manner, the reagent storage bin 901 is used to place a first type of reagent container and a second type of reagent container. The first type of reagent container is used to load a first type of reagent, and the second type of reagent container is used to load a second type of reagent. The reagent dispensing device 902 is used to aspirate the first type of reagent from the first type of reagent container and dispense it into the nucleic acid extraction container 10, and is used to aspirate the second type of reagent from the second type of reagent container and dispense it into the amplification reaction container.

[0258] As an implementation manner, the reagent storage bin 901 includes a first reagent storage component and a second reagent storage component. The first reagent storage component is used to store the first type of reagent. The reagent dispensing device 902 is used to aspirate the first type of reagent from the first reagent storage component and dispense it into the nucleic acid extraction container 10. The nucleic acid extraction device 100 is used to perform a nucleic acid extraction operation on the liquid in the nucleic acid extraction container 10 that at least contains a sample and the first type of reagent to obtain a nucleic acid extract. The second reagent storage component is used to store the second type of reagent. The reagent dispensing device 902 is used to aspirate the second type of reagent from the second reagent storage component and dispense it into the amplification reaction container. The amplification device 300 is used to amplify the liquid in the amplification reaction container that is at least made of the nucleic acid extract and the second type of reagent to obtain a test solution.

[0259] As an implementation manner, the above-mentioned sample analyzer 1 is an integrated molecular diagnostic instrument, which is used to detect RNA or DNA through nucleic acid extraction.

[0260] As an implementation manner, the nucleic acid extraction device 100 is divided into an upper part and a lower part. Among them, the upper part of the nucleic acid extraction device 100 is mainly used to realize functions such as injection of cleaning liquid, magnetic adsorption, suction and discharge of waste liquid, etc. The lower part of the nucleic acid extraction device 100 is mainly used to realize functions such as incubation, mixing, etc. In this implementation scheme, the key functional modules are designed with an upper and lower layout, and the components with similar functions are grouped into functional modules. For example, as an implementation manner, the lower part completes functions such as lysis, incubation, mixing, nucleic acid capture, nucleic acid washing, nucleic acid elution, etc. in nucleic acid extraction; while the upper part realizes functions such as liquid injection, magnetic separation, suction and discharge of waste liquid, etc. Then, by designing one or more manipulator functional modules, the nucleic acid extraction container 10 is grabbed and transferred between the upper and lower two functional components and each work station, so as to complete the complete nucleic acid extraction function. In this implementation scheme, all the work stations that need to be incubated are grouped into one functional component, and all the work stations that need to suck and inject liquid are grouped into another functional component, and then the two functional components are distributed up and down, and the nucleic acid extraction container 10 is transferred up and down. In this way, the parts with similar functions are grouped into one functional component, which can not only reduce the horizontal area, but also facilitate reducing the design and manufacturing difficulty of the nucleic acid extraction device 100.

[0261] Embodiment 2:

[0262] Referring to Figures 1 to 12 and Figure 19 As shown, the main difference between the sample analyzer 1 provided in this embodiment and that in Embodiment 1 lies in the setting manner of the fourth bearing member 121 of the second functional component 120, which is specifically reflected in: in Embodiment 1, the fourth bearing member 121 of the second functional component 120 can move relative to the frame assembly; while in this embodiment, the bearing member of the second functional component 120 is stationary relative to the frame assembly.

[0263] Specifically, the sample analyzer 1 provided in this embodiment includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly. The second functional component 120 includes a fourth carrier member 121, and the fourth carrier member 121 is fixed to the frame assembly for carrying the nucleic acid extraction container 10 to perform an incubation action, and at least one first transfer station 1215 and a plurality of accommodation cavities are formed on the fourth carrier member 121, and each accommodation cavity is used to accommodate one nucleic acid extraction container 10; the first carrier member 111 is fixed to the frame assembly; the transfer component 130 is at least used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the first carrier member 111, and the transfer component 130 is configured to be able to perform at least three-dimensional motion. The fourth carrier member 121 is installed on the frame assembly in a manner of being stationary relative to the frame assembly for carrying the nucleic acid extraction container 10 to perform an incubation action. The first functional component 110 includes a first carrier member 111 and a first pipetting member 118. The first carrier member 111 is installed on the frame assembly in a manner of being stationary relative to the frame assembly for carrying the nucleic acid extraction container 10 to perform a first magnetic adsorption and a first liquid suction action, and the first pipetting member 118 is used to perform a first liquid suction action on the nucleic acid extraction container 10 located on the first carrier member 111; the transfer component 130 is at least used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the first carrier member 111. In this implementation, the carrier members of the first functional component 110 and the second functional component 120 for carrying the nucleic acid extraction container 10 are both fixedly arranged relative to the frame assembly.

[0264] As an implementation manner, the second functional component 120 is formed with at least two first transfer stations 1215; the first functional component 110 further includes a second carrier member 112, a second pipetting member 114, a third carrier member 113, and a third pipetting member 117. Both the second carrier member 112 and the third carrier member 113 are mounted on the frame assembly in a stationary manner relative to the frame assembly for carrying the nucleic acid extraction container 10 to perform the first liquid suction action. The second pipetting member 114 is used to perform a second liquid suction action on the nucleic acid extraction container 10 located on the second carrier member 112 after the first liquid suction action is completed. The third pipetting member 117 is used to perform a second liquid injection action of injecting an elution reagent on the nucleic acid extraction container 10 located on the third carrier member 113 after the second liquid suction action is completed. The fourth carrier member 121 is formed with a lysis area 1211, a washing area 1212, a drying area 1213, and an elution area 1214. The number of the first transfer stations 1215 is the same as the sum of the numbers of the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214, and each of the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 is respectively provided with a first transfer station 1215. The transfer assembly 130 is configured to: transfer the nucleic acid extraction container 10 between the first transfer stations 1215 corresponding to the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 and the first carrier member 111, the second carrier member 112, and the third carrier member 113.

[0265] As an implementation manner, the first functional component 110 includes two first carrier members 111, one second carrier member 112, and one third carrier member 113. The fourth carrier member 121 is formed with one lysis area 1211, two washing areas 1212, one drying area 1213, and one elution area 1214. The number of the first transfer stations 1215 is five, and each of the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 is respectively provided with a first transfer station 1215.

[0266] As an implementation manner, the first carrier member 111, the second carrier member 112, and the third carrier member 113 are arranged side by side in sequence along the horizontal straight line direction, and the first transfer stations 1215 corresponding to the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 are distributed at intervals along the horizontal circumferential direction.

[0267] As an implementation manner, the transfer component 130 includes a manipulator, which can drive the nucleic acid extraction container 10 to move to the first bearing member 111, the second bearing member 112, the third bearing member 113 and the first transfer stations 1215 corresponding to the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 respectively.

[0268] In this implementation scheme, the bearing members of the first functional component 110 and the second functional component 120 for bearing the nucleic acid extraction container 10 are fixedly arranged relative to the frame component. The transfer of the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120 is realized by the movement of the transfer component 130.

[0269] Except for the above, other parts of the sample analyzer 1 provided in this embodiment may refer to Embodiment 1, which will not be elaborated here.

[0270] Embodiment 3:

[0271] The main difference between the sample analyzer 1 provided in this embodiment and that in Embodiment 2 lies in the setting manner of the bearing members of the first functional component 110, specifically: in Embodiment 2, the bearing members of the first functional component 110 are arranged side by side in a horizontal straight line direction; while in this embodiment, the bearing members of the first functional component 110 are arranged at intervals in a horizontal circumferential direction.

[0272] Specifically, in the sample analyzer 1 provided in this embodiment, at least two first transfer stations 1215 are arranged at intervals in a horizontal circumferential direction in sequence, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged at intervals in a horizontal circumferential direction in sequence, the transfer component 130 includes a single first clamping member, a single second driving member, and a single rotary driving member. The first clamping member is used to clamp and release the nucleic acid extraction container 10, the rotary driving member is used to drive the first clamping member and the second driving member to rotate horizontally so that the first clamping member rotates to the same orientation as the first transfer station 1215, the first bearing member 111, the second bearing member 112, and the third bearing member 113 respectively, and the second driving member is used to drive the first clamping member to perform two-dimensional linear motion so that the first clamping member moves to the first transfer station 1215, the first bearing member 111, the second bearing member 112, and the third bearing member 113 respectively to clamp and release the nucleic acid extraction container 10. In this implementation scheme, the upper first bearing member 111, the second bearing member 112, and the third bearing member 113 are distributed at intervals in a horizontal circumferential direction, and the first transfer stations 1215 corresponding to the lysis area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 in the lower layer are distributed at intervals in a horizontal circumferential direction. The transfer component 130 rotates to make the first clamping member face each station respectively.

[0273] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment may refer to Embodiment 1 and Embodiment 2, which will not be elaborated here.

[0274] Embodiment 4:

[0275] The sample analyzer 1 provided in this embodiment is mainly different from that in Embodiment 3 in the setting manner of the transfer assembly 130, specifically reflected in: in Embodiment 3, the transfer assembly 130 includes a single first clamping member, a single second driving member and a single rotation driving member, and the first clamping member is respectively aligned with each first transfer station 1215 in a rotating manner; while in this embodiment, a first clamping member is provided corresponding to each first transfer station 1215, and the first clamping member can be aligned with the first transfer station 1215 without rotation.

[0276] Specifically, in the sample analyzer 1 provided in this embodiment, at least two first transfer stations 1215 are sequentially arranged at intervals along the horizontal circumferential direction, the first carrying member 111, the second carrying member 112, and the third carrying member 113 are sequentially arranged at intervals along the horizontal circumferential direction, the transfer assembly 130 includes at least two first clamping members and at least two second driving members, the number of the first clamping members and the number of the second driving members are the same as the number of the first transfer stations 1215, each first clamping member is used for clamping and releasing the nucleic acid extraction container 10 between one of the first carrying member 111, the second carrying member 112, and the third carrying member 113 and a first transfer station 1215, and each second driving member is correspondingly used for driving a first clamping member to perform two-dimensional linear motion so that the first clamping member moves to one of the first carrying member 111, the second carrying member 112, and the third carrying member 113 and a first transfer station 1215 to clamp and release the nucleic acid extraction container 10. In this implementation scheme, a first clamping member and a second driving member are provided corresponding to each first transfer station 1215, and the first clamping member does not need to rotate.

[0277] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment may refer to Embodiments 1 to 3, which will not be elaborated here.

[0278] Embodiment 5:

[0279] The sample analyzer 1 provided in this embodiment is mainly different from that in Embodiment 1 in the setting manner of the carrying member of the first functional assembly 110, specifically reflected in: in Embodiment 1, the carrying member of the first functional assembly 110 is stationary relative to the frame assembly; while in this embodiment, the carrying member of the first functional assembly 110 can move relative to the frame assembly.

[0280] Specifically, in the sample analyzer 1 provided in this embodiment, it includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly; the second functional component 120 includes a fourth carrying member 121 and a first driving member 122. The fourth carrying member 121 is movably installed on the frame assembly to carry out an incubation action for the nucleic acid extraction container 10, and at least one first transfer station 1215 and a plurality of accommodating cavities are formed on the fourth carrying member 121, and each accommodating cavity is used to accommodate one nucleic acid extraction container 10. The first driving member 122 is used to drive the fourth carrying member 121 to move relative to the frame assembly so that the fourth carrying member 121 drives the nucleic acid extraction container 10 to move to the first transfer station 1215 respectively; the first functional component 110 includes a first carrying member 111, a first liquid transfer member 118 and a second driving member. The first carrying member 111 is movably installed on the frame assembly to carry out a first liquid suction action for the nucleic acid extraction container 10. The second driving member is used to drive the first carrying member 111 to move relative to the frame assembly so that the first carrying member 111 drives the nucleic acid extraction container 10 to move to the second transfer station. The first liquid transfer member 118 is used to carry out a first liquid suction action on the nucleic acid extraction container 10 located on the first carrying member 111. The transfer assembly 130 is used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the second transfer station.

[0281] As an implementation manner, the first functional component 110 further includes a second carrying member 112, a third carrying member 113, a fourth driving member and a fifth driving member. A lysis area 1211, a washing area 1212, a drying area 1213 and an elution area 1214 are formed on the fourth carrying member 121. The number of the first transfer stations 1215 is one, and the number of the second transfer stations is one. The second carrying member 112 and the third carrying member 113 are respectively movably installed on the frame assembly. The fourth driving member is used to drive the second carrying member 112 to move relative to the frame assembly so that the second carrying member 112 drives the nucleic acid extraction container 10 to move to the second transfer station. The fifth driving member is used to drive the third carrying member 113 to move relative to the frame assembly so that the third carrying member 113 drives the nucleic acid extraction container 10 to move to the second transfer station.

[0282] As an implementation manner, the first functional component 110 includes two first carrying members 111, one second carrying member 112 and one third carrying member 113. A lysis area 1211, two washing areas 1212, a drying area 1213 and an elution area 1214 are formed on the fourth carrying member 121.

[0283] As an implementation manner, the first carrier member 111, the second carrier member 112, and the third carrier member 113 are arranged side by side in sequence along the horizontal straight line direction, and the first transfer stations 1215 corresponding to the cracking area 1211, the washing area 1212, the drying area 1213, and the elution area 1214 are distributed at intervals along the horizontal circumferential direction.

[0284] As an implementation manner, the transfer assembly 130 includes a manipulator, and the manipulator can drive the nucleic acid extraction container 10 to move to the first transfer station 1215 and the second transfer station respectively.

[0285] In this implementation scheme, the carrier members of the first functional component 110 and the second functional component 120 for carrying the nucleic acid extraction container 10 are both movably arranged relative to the frame assembly.

[0286] Except for the above, other parts of the sample analyzer 1 provided in this embodiment can refer to Embodiments 1 to 4, which will not be elaborated here.

[0287] Embodiment 6:

[0288] The sample analyzer 1 provided in this embodiment is mainly different from that in Embodiment 1 in the setting manner of the carrier members of the first functional component 110 and the second functional component 120. Specifically, in Embodiment 1, the carrier member of the first functional component 110 is stationary relative to the frame assembly, and the carrier member of the second functional component 120 can move relative to the frame assembly; while in this embodiment, the carrier member of the first functional component 110 can move relative to the frame assembly, and the carrier member of the second functional component 120 is stationary relative to the frame assembly.

[0289] Specifically, the sample analyzer 1 provided in this embodiment includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly; the second functional component 120 includes a fourth carrier member 121, and the fourth carrier member 121 is installed on the frame assembly in a manner stationary relative to the frame assembly to carry the nucleic acid extraction container 10 to perform an incubation action; the first functional component 110 includes a first carrier member 111, a first liquid transfer member 118, and a second driving member. The first carrier member 111 is movably installed on the frame assembly to carry the nucleic acid extraction container 10 to perform a first liquid suction action. The second driving member is used to drive the first carrier member 111 to move relative to the frame assembly so that the first carrier member 111 drives the nucleic acid extraction container 10 to move to the second transfer station. The first liquid transfer member 118 is used to perform a first liquid suction action on the nucleic acid extraction container 10 located on the first carrier member 111. The transfer assembly 130 is used to transfer the nucleic acid extraction container 10 between the fourth carrier member 121 and the second transfer station. In this implementation scheme, the carrier member of the second functional component 120 located below does not move, and the carrier member of the first functional component 110 located above moves.

[0290] As an implementation manner, the first functional component 110 further includes a second carrier member 112, a third carrier member 113, a fourth driving member, and a fifth driving member. The fourth carrier member 121 is formed with a lysis zone 1211, a washing zone 1212, a drying zone 1213, and an elution zone 1214, and the number of the second transfer stations is one. The second carrier member 112 and the third carrier member 113 are respectively movably mounted on the frame assembly. The fourth driving member is used to drive the second carrier member 112 to move relative to the frame assembly so that the second carrier member 112 drives the nucleic acid extraction container 10 to move to the second transfer station. The fifth driving member is used to drive the third carrier member 113 to move relative to the frame assembly so that the third carrier member 113 drives the nucleic acid extraction container 10 to move to the second transfer station.

[0291] As an implementation manner, the first functional component 110 includes two first carrier members 111, one second carrier member 112, and one third carrier member 113. The fourth carrier member 121 is formed with one lysis zone 1211, two washing zones 1212, one drying zone 1213, and one elution zone 1214.

[0292] As an implementation manner, the transfer component 130 includes a manipulator, and the manipulator can respectively drive the nucleic acid extraction container 10 to move to the lysis zone 1211, the washing zone 1212, the drying zone 1213, the elution zone 1214, and the second transfer station.

[0293] Except for the above, other parts of the sample analyzer 1 provided in this embodiment may refer to Embodiments 1 to 5, which will not be elaborated here.

[0294] Embodiment 7:

[0295] The sample analyzer 1 provided in this embodiment is mainly different from that in Embodiment 1 in that the functional components of the upper and lower parts of the nucleic acid extraction device 100 are defined differently. Specifically, in Embodiment 1, it is defined that one of the functional components of the upper part and the lower part of the nucleic acid extraction device 100 is used to at least perform magnetic adsorption and liquid suction actions on the liquid in the nucleic acid extraction container 10, and the other is at least used to incubate the liquid in the nucleic acid extraction container 10; while in this embodiment, the specific functional actions of the functional components of the upper and lower parts of the nucleic acid extraction device 100 are not limited, as long as the upper and lower parts are used to perform different functional actions or to perform the same functional actions in sequence.

[0296] Specifically, the sample analyzer 1 provided in this embodiment includes a sample dispensing device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300, and a detection device 400. The sample dispensing device 200 is configured to aspirate a sample from a sample container and dispense at least a portion of the aspirated sample into a nucleic acid extraction container 10. The nucleic acid extraction device 100 is configured to perform nucleic acid extraction on a liquid in the nucleic acid extraction container 10 that contains at least the sample and a reagent to obtain a nucleic acid extract. The pipetting device 700 is configured to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container. The amplification device 300 is configured to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution. The detection device 400 is configured to detect the test solution. Among them, the nucleic acid extraction device 100 includes a first functional component 110, a second functional component 120, and a transfer component 130. The first functional component 110 is disposed above or below the second functional component 120. The transfer component 130 is configured to transfer the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120. The first functional component 110 is at least configured to carry the nucleic acid extraction container 10 to perform a first functional action, and the second functional component 120 is at least configured to carry the nucleic acid extraction container 10 to perform a second functional action to perform nucleic acid extraction. The first functional action and the second functional action are different functional actions or the same functional action performed successively. In this embodiment, the first functional component 110 and the second functional component 120 may be: one is configured to perform magnetic adsorption and liquid aspiration actions on the liquid in the nucleic acid extraction container 10, and the other is configured to incubate the liquid in the nucleic acid extraction container 10; or it may not necessarily be: one is configured to perform magnetic adsorption and liquid aspiration actions on the liquid in the nucleic acid extraction container 10, and the other is configured to incubate the liquid in the nucleic acid extraction container 10. For example, both the first functional component 110 and the second functional component 120 may be integrated with components having incubation, magnetic adsorption, and liquid aspiration functions.

[0297] As an implementation manner, the first functional action includes at least one of a liquid aspiration action, a liquid injection action, and a magnetic adsorption action. Grouping parts with similar functions into one functional component is conducive to reducing the design and manufacturing difficulty.

[0298] As an implementation manner, the second functional action includes at least one of an incubation action and a mixing action. Grouping parts with similar functions into one functional component is conducive to reducing the design and manufacturing difficulty.

[0299] As an implementation manner, the second functional component 120 includes a fourth bearing member 121 and a mixing member 140. The fourth bearing member 121 is used to bear the nucleic acid extraction container 10 to perform an incubation action. The mixing member 140 is used to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the fourth bearing member 121. Among them, the mixing member 140 at least includes a horizontally extending first magnet array 141 and a second magnet array 142. Each magnet array respectively includes a plurality of magnets spacedly installed on a magnet bracket 143. The fourth bearing member 121 includes a container receiving portion for placing the nucleic acid extraction container 10. The fourth bearing member 121 and the mixing member 140 are arranged relative to each other such that when the nucleic acid extraction container 10 is received in the container receiving portion, at least the bottom of the nucleic acid extraction container 10 is located between the first magnet array 141 and the second magnet array 142. The fourth bearing member 121 and the mixing member 140 can move horizontally relative to each other, so that the mixing member 140 can mix the liquid containing at least the sample and magnetic beads in the nucleic acid extraction container 10 received in the container receiving portion. The fourth bearing member 121 and the mixing member 140 are also arranged relative to each other such that when the fourth bearing member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0300] As an implementation manner, the mixing member 140 is used to generate a magnetic field to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the fourth bearing member 121.

[0301] As an implementation manner, the second functional component 120 further includes a third driving member. The mixing member 140 includes a movable magnet bracket 143 and a plurality of magnets fixed on the magnet bracket 143. The third driving member is used to drive the magnet bracket 143 to move, so as to drive the plurality of magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth bearing member 121.

[0302] As an implementation manner, the third driving member is used to drive the magnet bracket 143 to drive the plurality of magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth bearing member 121, mainly for enabling the magnetic beads in the nucleic acid extraction container 10 placed on the fourth bearing member 121 to perform three-dimensional movement in the liquid of the nucleic acid extraction container 10 under the action of the moving magnets.

[0303] As an implementation manner, the second functional component 120 includes a fourth bearing member 121, a mixing member 140, and a third driving member. The fourth bearing member 121 is used to carry the nucleic acid extraction container 10 to perform an incubation action. The mixing member 140 is used to generate a magnetic field to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container 10 located on the fourth bearing member 121. The third driving member is used to drive the relative movement of the fourth bearing member 121 and the mixing member 140, so that the mixing member 140 generates an alternating magnetic field relative to the nucleic acid extraction container 10 on the fourth bearing member 121, so that the magnetic beads in the nucleic acid extraction container 10 can perform three-dimensional movement in the liquid of the nucleic acid extraction container 10 under the action of the alternating magnetic field. Or,

[0304] As an implementation manner, the second functional component 120 includes a fourth bearing member 121, a mixing member 140, and a third driving member. The fourth bearing member 121 is used to carry the nucleic acid extraction container 10 to perform an incubation action. The fourth bearing member 121 at least includes a lysis area 1211. The nucleic acid extraction container 10 undergoes a lysis and capture process in the lysis area 1211. In this lysis and capture process, the cells in the sample of the nucleic acid extraction container 10 are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container 10. The mixing member 140 is used to generate a magnetic field that at least covers the lysis area 1211 to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container 10 located in the lysis area 1211. The third driving member is used to drive the relative movement of the fourth bearing member 121 and the mixing member 140, so that the magnetic beads in the nucleic acid extraction container 10 in the lysis area 1211 move, so that the magnetic beads can capture the released nucleic acids.

[0305] For the sample analyzer 1 provided in this embodiment, by arranging one of the first functional component 110 and the second functional component 120 in the nucleic acid extraction device 100 that is used to perform different functional actions or used to perform the same functional action successively above or below the other, and transferring the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120 through the transfer component 130, that is: the first functional component 110 and the second functional component 120 that are used to perform different functional actions on the liquid in the nucleic acid extraction container 10 or used to perform the same functional action successively are arranged vertically up and down. The nucleic acid extraction container 10 is transferred to the first functional component 110 and the second functional component 120 respectively through the transfer component 130. By adopting this setting scheme, the different functional components of the nucleic acid extraction device 100 can be fully utilized to arrange vertically, so as to facilitate reducing the horizontal occupied space of the nucleic acid extraction device 100, and further facilitate the miniaturization design of the sample analyzer 1 and improve the space utilization of the laboratory where the sample analyzer 1 is located.

[0306] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment may refer to Embodiments 1 to 6, which will not be elaborated here.

[0307] Embodiment 8:

[0308] The main difference between the sample analyzer 1 provided in this embodiment and that in Embodiment 1 lies in the different definitions of the upper and lower parts of the nucleic acid extraction device 100. Specifically, in Embodiment 1, it is defined that one of the functional components in the upper part and the lower part of the nucleic acid extraction device 100 is used to perform at least magnetic adsorption and liquid suction actions on the liquid in the nucleic acid extraction container 10, and the other is at least used to incubate the liquid in the nucleic acid extraction container 10; while in this embodiment, it is mainly defined that the nucleic acid extraction device 100 has two workstations arranged vertically up and down.

[0309] Specifically, the sample analyzer 1 provided in this embodiment includes a sample dispensing device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300, and a detection device 400. The sample dispensing device 200 is used to aspirate a sample from a sample container and dispense at least a part of the aspirated sample into the nucleic acid extraction container 10; the nucleic acid extraction device 100 is used to extract nucleic acids from the liquid in the nucleic acid extraction container 10 that at least contains the sample and the reagent to obtain a nucleic acid extract; the pipetting device 700 is used to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container; the amplification device 300 is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; the detection device 400 is used to detect the test solution. Among them, the nucleic acid extraction device 100 has at least two workstations, and each workstation is respectively used to carry the nucleic acid extraction container 10 to perform a processing action for nucleic acid extraction. At least two workstations are distributed vertically up and down, and the processing actions include at least one of an incubation action, a mixing action, a magnetic adsorption action, a liquid suction action, and a liquid injection action. In this implementation scheme, setting at least two workstations of the nucleic acid extraction device 100 vertically can also achieve the purpose of reducing the horizontal occupied area of the nucleic acid extraction device 100.

[0310] As an implementation manner, the nucleic acid extraction of the liquid that at least contains the sample and the reagent by the nucleic acid extraction device 100 includes: the process of lysing the liquid that at least contains the sample and the reagent and the subsequent processing after lysis.

[0311] As an implementation manner, the nucleic acid extraction device 100 has a first-layer space and a second-layer space, and the first-layer space is arranged above or below the second-layer space; an aspiration and injection liquid station, a single aspiration liquid station, and a single injection liquid station are provided in the first-layer space; a lysis incubation station, a washing incubation station, a drying incubation station, and an elution incubation station are provided in the second-layer space; wherein, the lysis incubation station is used for carrying the nucleic acid extraction container 10 to perform a lysis incubation action; the washing incubation station is used for carrying the nucleic acid extraction container 10 to perform a washing incubation action; the drying incubation station is used for carrying the nucleic acid extraction container 10 to perform a drying incubation action; the elution incubation station is used for carrying the nucleic acid extraction container 10 to perform an elution incubation action; the aspiration and injection liquid station is used for carrying the nucleic acid extraction container 10 to perform a first aspiration liquid action and a first injection liquid action of injecting a washing solution; the single aspiration liquid station is used for carrying the nucleic acid extraction container 10 to perform a second aspiration liquid action; the single injection liquid station is used for carrying the nucleic acid extraction container 10 to perform a second injection liquid action of injecting an elution reagent.

[0312] For the sample analyzer 1 provided in this embodiment, by arranging at least two stations in the nucleic acid extraction device 100 to be vertically distributed up and down, and setting each station to be used for carrying the nucleic acid extraction container 10 to perform at least one processing action among incubation action, mixing action, magnetic adsorption action, aspiration liquid action, and injection liquid action, that is, setting at least two stations for carrying the nucleic acid extraction container 10 to perform processing actions to be arranged up and down in the vertical direction. By adopting this setting scheme, the multiple stations of the nucleic acid extraction device 100 can be arranged by making full use of the vertical space, so as to facilitate reducing the horizontal occupied space of the nucleic acid extraction device 100, and further facilitate the miniaturization design of the sample analyzer 1 and improve the space utilization of the laboratory where the sample analyzer 1 is located.

[0313] Except for the above, other parts of the sample analyzer 1 provided in this embodiment can refer to Embodiments 1 to 7, which will not be elaborated here.

[0314] Embodiment Nine:

[0315] The main difference between the sample analyzer 1 provided in this embodiment and Embodiment 1 lies in the different ways of dividing the upper and lower part functional components of the nucleic acid extraction device 100, which is specifically reflected in: in Embodiment 1, the upper and lower parts of the nucleic acid extraction device 100 are divided according to similar functions, the upper part is the structural integration related to the aspiration and injection liquid function, and the lower part is the structural integration related to incubation and mixing; while in this embodiment, the upper and lower parts of the nucleic acid extraction device 100 are divided according to the sequence of actions. After the nucleic acid extraction container 10 completes the incubation and aspiration and injection liquid actions at the same time at a station in the lower part, it is then transferred to a station in the upper part to complete the incubation and aspiration and injection liquid actions at the same time.

[0316] Specifically, the sample analyzer 1 provided in this embodiment includes a sample dispensing device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300, and a detection device 400. The sample dispensing device 200 is configured to aspirate a sample from a sample container and dispense at least a portion of the aspirated sample into a nucleic acid extraction container 10. The nucleic acid extraction device 100 is configured to perform a nucleic acid extraction operation on the liquid in the nucleic acid extraction container 10 that contains at least the sample and a reagent to obtain a nucleic acid extract. The pipetting device 700 is configured to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container. The amplification device 300 is configured to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution. The detection device 400 is configured to detect the test solution. Among them, the nucleic acid extraction device 100 includes a lysis module, a washing module, an elution module, and a transfer assembly 130. At least two of the lysis module, the washing module, and the elution module are distributed in the vertical direction. The lysis module is configured to carry the nucleic acid extraction container 10 to perform a lysis operation. The washing module is configured to carry the nucleic acid extraction container 10 to perform a washing operation. The elution module is configured to carry the nucleic acid extraction container 10 to perform an elution operation. The transfer assembly 130 is configured to sequentially transfer the nucleic acid extraction container 10 to the lysis module, the washing module, and the elution module.

[0317] As an implementation manner, the nucleic acid extraction device 100 performing nucleic acid extraction on the liquid that contains at least the sample and a reagent includes: a process of lysing the liquid that contains at least the sample and a reagent and performing subsequent processing after lysis.

[0318] As an implementation manner, the nucleic acid extraction device 100 further includes a drying module. The drying module is disposed below or above at least one of the lysis module, the washing module, and the elution module. The drying module is configured to carry the nucleic acid extraction container 10 to perform a drying operation. The transfer assembly 130 is configured to sequentially transfer the nucleic acid extraction container 10 to the lysis module, the washing module, the drying module, and the elution module.

[0319] As an implementation manner, according to the chronological order in the nucleic acid extraction process, the nucleic acid extraction device 100 can be split into multiple functional modules. The nucleic acid extraction container 10 is sequentially transferred to different functional modules without performing a turning-back action. For example, the washing module includes both a liquid aspiration / dispensing component and an incubation component and a mixing component, and the elution module includes both a liquid aspiration / dispensing component and an incubation component and a mixing component.

[0320] As an implementation manner, the nucleic acid extraction device 100 further includes a drying module. The drying module is disposed below or above at least one of the lysis module, the washing module, and the elution module. The drying module is configured to carry the nucleic acid extraction container 10 to perform a drying operation. The transfer assembly 130 is configured to sequentially transfer the nucleic acid extraction container 10 to the lysis module, the washing module, the drying module, and the elution module.

[0321] As an implementation manner, the number of cleaning modules can be two, one, three, or more. When there are two cleaning modules, the transfer component 130 is used to sequentially transfer the nucleic acid extraction container 10 to the lysis module, one cleaning module, the other cleaning module, the drying module, and the elution module.

[0322] In the first embodiment, all the incubation structures and mixing structures are integrated into one functional component; while in this embodiment, all the incubation structures can be split into multiple sub-incubation blocks, and other corresponding functional components can be arranged corresponding to each sub-incubation block, such as liquid injection / aspiration components, temperature control components, magnetic attraction components, mixing components, etc.

[0323] For the sample analyzer 1 provided in this embodiment, by arranging at least two of the lysis module, the cleaning module, and the elution module in the nucleic acid extraction device 100 vertically one above the other, that is, arranging the functional modules for carrying the nucleic acid extraction container 10 to perform different sequential functional actions vertically one above the other. Adopting this arrangement scheme can make full use of the vertical space to arrange multiple functional modules of the nucleic acid extraction device 100, thereby facilitating reducing the horizontal occupied space of the nucleic acid extraction device 100, and further facilitating the miniaturized design of the sample analyzer 1 and improving the space utilization of the laboratory where the sample analyzer 1 is located.

[0324] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to Embodiments 1 to 8, which will not be elaborated here.

[0325] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structural transformation made under the inventive concept of the present invention by using the content of the specification and drawings of the present invention, or directly / indirectly applied in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container; A nucleic acid extraction device, the nucleic acid extraction device is used to extract nucleic acid from the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extraction solution, wherein the reagent contains magnetic beads; A liquid transfer device, the liquid transfer device is used to transfer the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container; an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device, the detection device is used to detect the liquid to be tested; Among them, the nucleic acid extraction device includes a first functional component, a second functional component and a transfer component, the first functional component is arranged above or below the second functional component, and the transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component to perform the nucleic acid extraction to obtain the nucleic acid extract; the first functional component is used to at least perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container, and the second functional component is at least used to incubate the liquid in the nucleic acid extraction container.

2. The sample analyzer according to claim 1, wherein: The first functional component includes a first bearing member, a first pipetting member and a first magnetic attraction member, the first bearing member is used to bear the nucleic acid extraction container, the first magnetic attraction member is used to perform a first magnetic adsorption on the liquid in the nucleic acid extraction container located on the first bearing member and containing at least a sample and a reagent so as to adsorb the magnetic beads in the nucleic acid extraction container, the first pipetting member is used to perform a first liquid aspiration action on the liquid in the nucleic acid extraction container located on the first bearing member and subjected to the first magnetic adsorption by the first magnetic attraction member, the transfer component is used to transfer the nucleic acid extraction container between the first bearing member and the second functional component, the magnetic adsorption includes the first magnetic adsorption, and the liquid aspiration action includes the first liquid aspiration action.

3. The sample analyzer according to claim 2, wherein: The first functional component further includes a liquid injection component, which is used to perform a first liquid injection action of injecting a cleaning liquid into the nucleic acid extraction container located on the first supporting component.

4. The sample analyzer according to claim 3, wherein: The first functional component also includes a second bearing member, a second pipetting member and a second magnetic attraction member, the second bearing member is used to bear the nucleic acid extraction container, the second magnetic attraction member is used to perform a second magnetic attraction on the liquid in the nucleic acid extraction container located on the second bearing member and containing at least a cleaning liquid, the second pipetting member is used to perform a second liquid aspiration action on the liquid in the nucleic acid extraction container located on the second bearing member and subjected to the second magnetic attraction by the second magnetic attraction member; the transfer component is also used to transfer the nucleic acid extraction container between the second bearing member and the second functional component, the magnetic attraction also includes the second magnetic attraction, and the liquid aspiration action also includes the second liquid aspiration action.

5. The sample analyzer according to claim 4, characterized in that: The first functional component also includes a third bearing member and a third pipetting member, the third bearing member is used to bear the nucleic acid extraction container, the third pipetting member is used to perform a second liquid injection action of injecting elution reagent into the nucleic acid extraction container located on the third bearing member; the transfer component is also used to transfer the nucleic acid extraction container between the third bearing member and the second functional component.

6. The sample analyzer according to claim 5, characterized in that: The sample analyzer further includes a first lifting component; the first lifting component is connected to the second magnetic attraction component, and the first lifting component is used to drive the second magnetic attraction component to move up and down relative to the nucleic acid extraction container.

7. The sample analyzer according to claim 4, characterized in that: The nucleic acid extraction container is formed with a reaction hole, a first pipette tip hole and a second pipette tip hole, the reaction hole is used to carry samples and reagents for nucleic acid extraction, the first pipette tip hole is used to carry a first pipette tip, the second pipette tip hole is used to carry a second pipette tip, and the volume of the first pipette tip is greater than the volume of the second pipette tip; The first liquid transfer component includes a first suction head connecting component and a first suction and discharge power component; The first pipette tip connecting component is used to be detachably connected to the first pipette tip so as to aspirate liquid through the first pipette tip under the action of power provided by the first suction and discharge power component; The third pipetting component includes a second pipette tip connecting component and a second suction and discharge power component. The second pipette tip connecting component is used to be detachably connected to the second pipette tip so as to aspirate and inject liquid through the second pipette tip under the power provided by the second suction and discharge power component.

8. The sample analyzer according to claim 7, wherein: The nucleic acid extraction container is further formed with a waste liquid hole, and the first liquid transfer member and the second liquid transfer member are further used to discharge the liquid sucked from the reaction hole to the waste liquid hole.

9. The sample analyzer according to claim 3, characterized in that: The number of the first bearing members is at least two, and each of the first bearing members is correspondingly provided with at least one liquid injection member and at least one first liquid transfer member.

10. The sample analyzer according to claim 5, characterized in that: The first bearing member, the second bearing member and the third bearing member are arranged side by side in a horizontal direction.

11. The sample analyzer according to claim 5, characterized in that: The second functional component includes a fourth bearing member and a heating member, the fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action, and the heating member is used to heat the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth bearing member; the fourth bearing member includes a lysis zone, a washing zone, a drying zone and an elution zone, and the lysis zone, the washing zone, the drying zone and the elution zone are respectively formed with a accommodating cavity for placing the nucleic acid extraction container, and the transfer component is used to transfer the nucleic acid extraction container between the lysis zone, the washing zone and the first bearing member, between the washing zone, the drying zone and the second bearing member, and between the drying zone, the elution zone and the third bearing member.

12. The sample analyzer according to claim 11, characterized in that: The fourth supporting member includes an incubation tray, and the heating member is used to heat the nucleic acid extraction container on the incubation tray; the incubation tray is formed with the lysis zone, the washing zone, the drying zone and the elution zone, and a first transfer station is formed on the incubation tray, the incubation tray is used to dispatch the nucleic acid extraction containers located in the lysis zone, the washing zone, the drying zone and the elution zone to the first transfer station respectively, and the transfer component is used to dispatch the nucleic acid extraction container located in the first transfer station to the first functional component.

13. The sample analyzer according to claim 11, wherein: The sample analyzer further includes a controller, which is configured to: Controlling the transfer component to dispatch the nucleic acid extraction container after performing the incubation action in the lysis area to the first carrying member; Controlling the first liquid transfer member to perform the first liquid aspirating action on the liquid in the nucleic acid extraction container in the first carrying member and controlling the liquid injection member to perform the first liquid injection action on the nucleic acid extraction container in the first carrying member; Controlling the transfer component to transfer the nucleic acid extraction container after performing the first liquid injection action to the washing area to perform the incubation action; Controlling the transfer component to transfer the nucleic acid extraction container after performing the incubation action in the washing area to the second carrying member; Controlling the second liquid transfer member to perform the second liquid aspiration action on the liquid in the nucleic acid extraction container in the second carrying member; Controlling the transfer component to transfer the nucleic acid extraction container after performing the second liquid aspiration action to the drying area to perform a drying action on the nucleic acid extraction container; Controlling the transfer component to transfer the nucleic acid extraction container after the drying action is performed in the drying area to the third carrying member; Controlling the third liquid transfer member to perform the second liquid injection action on the nucleic acid extraction container in the third carrying member; The transfer component is controlled to transport the nucleic acid extraction container after performing the second liquid injection action to the elution area to perform the elution action.

14. The sample analyzer according to claim 11, wherein: The position of the bottom of the accommodating chamber in the elution zone is higher than the position of the bottom of the accommodating chamber in the cracking zone, higher than the position of the bottom of the accommodating chamber in the washing zone, and higher than the position of the bottom of the accommodating chamber in the drying zone; and / or, The position of the bottom of the accommodating chamber in the cracking zone is at the same height as the position of the bottom of the accommodating chamber in the washing zone and the position of the bottom of the accommodating chamber in the drying zone.

15. The sample analyzer according to any one of claims 1 to 14, characterized in that: The second functional component includes a fourth supporting member, a heating member and a mixing member. The fourth supporting member is used to support the nucleic acid extraction container to perform an incubation action. The heating member is used to heat the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth supporting member. The mixing member is used to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth supporting member.

16. The sample analyzer according to claim 15, characterized in that: The second functional component further includes a first driving member, and the first driving member is used to drive the fourth bearing member to move so that the fourth bearing member drives the nucleic acid extraction container to move; The mixing component includes a plurality of magnets, and the plurality of magnets are alternately distributed on opposite sides of the movement track of the nucleic acid extraction container along the horizontal direction.

17. The sample analyzer according to claim 15, wherein: The mixing component comprises at least a first magnet array and a second magnet array extending horizontally, and each of the magnet arrays comprises a plurality of magnets installed at intervals on a magnet bracket; The fourth bearing member comprises a container receiving portion for placing the nucleic acid extraction container; The fourth bearing member and the mixing member are arranged relative to each other so that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array; The fourth bearing member and the mixing member can move horizontally relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion; The fourth supporting member and the mixing member are further arranged relative to each other so that when the fourth supporting member and the mixing member move horizontally relative to each other, at least two magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

18. The sample analyzer according to claim 17, wherein: The at least two magnets in the mixing member have different heights relative to the fourth bearing member; or, The at least two magnets in the mixing member are installed at different heights on the magnet support, so that the at least two magnets in the mixing member have different heights relative to the fourth bearing member.

19. The sample analyzer according to claim 15, wherein: The second functional component further includes a third driving member, the mixing member includes a movable magnet bracket and a plurality of magnets fixed on the magnet bracket, wherein the third driving member is used to drive the magnet bracket to move, so as to drive the plurality of magnets fixed on the magnet bracket to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the moving magnet; or, The second functional component further includes a third driving member, the mixing member is used to generate a magnetic field, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container can perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or, The second functional component also includes a third driving member, the fourth bearing member includes at least a lysis zone, the nucleic acid extraction container undergoes a lysis capture link in the lysis zone, in which cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container, the mixing member is used to generate a magnetic field covering at least the lysis zone, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis zone move, so that the magnetic beads capture the released nucleic acids.

20. The sample analyzer according to any one of claims 2 to 14, characterized in that: The sample analyzer further comprises a frame assembly, and the nucleic acid extraction device is mounted on the frame assembly; The second functional component includes a fourth bearing member and a first driving member, the fourth bearing member can be movably mounted on the frame assembly to carry the nucleic acid extraction container to perform an incubation action, and the fourth bearing member is formed with at least one first transfer station and a plurality of accommodating cavities, each of the accommodating cavities is used to accommodate one nucleic acid extraction container, and the first driving member is used to drive the fourth bearing member to move relative to the frame assembly so that the fourth bearing member drives the plurality of nucleic acid extraction containers to move to the first transfer station respectively; The first bearing member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer station and the first bearing member.

21. The sample analyzer according to any one of claims 2 to 14, characterized in that: The sample analyzer further comprises a frame assembly, and the nucleic acid extraction device is mounted on the frame assembly; The second functional component includes a fourth bearing member, which is fixed to the frame component to carry the nucleic acid extraction container to perform an incubation action, and the fourth bearing member is formed with at least one first transfer station and a plurality of accommodating cavities, each of which is used to accommodate one nucleic acid extraction container; The first bearing member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer station and the first bearing member, and the transfer assembly is configured to perform at least three-dimensional movement.

22. The sample analyzer according to any one of claims 1 to 14, characterized in that: The sample analyzer also includes: A container providing device, the container providing device is used to provide the nucleic acid extraction container and the amplification reaction container; A reagent storage bin, the reagent storage bin is used to store a first type of reagent and a second type of reagent, the first type of reagent is used to react with a sample to obtain a nucleic acid extract, and the second type of reagent is used to react with the nucleic acid extract to perform amplification; a reagent dispensing device, the reagent dispensing device being used to dispense the first type of reagent stored in the reagent storage bin into the nucleic acid extraction container, and being used to dispense the second type of reagent stored in the reagent storage bin into the amplification reaction container; A scheduling device, the scheduling device is at least used to schedule the nucleic acid extraction container provided by the container providing device to the nucleic acid extraction device and to schedule the amplification reaction container provided by the container providing device to the amplification device.

23. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container; A nucleic acid extraction device, the nucleic acid extraction device is used to extract nucleic acid from the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extraction solution; A liquid transfer device, the liquid transfer device is used to transfer the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container; an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device, the detection device is used to detect the liquid to be tested; The nucleic acid extraction device comprises a first functional component, a second functional component and a transfer component, wherein the first functional component is arranged above or below the second functional component, the transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component, the first functional component is at least used to carry the nucleic acid extraction container to perform a first functional action and the second functional component is at least used to carry the nucleic acid extraction container to perform a second functional action to perform nucleic acid extraction; The first functional action and the second functional action are different functional actions or the same functional action executed successively.

24. The sample analyzer according to claim 23, wherein: The first functional action includes at least one of a liquid suction action, a liquid injection action, and a magnetic adsorption action; and / or, The second functional action includes at least one of an incubation action and a mixing action.

25. The sample analyzer according to claim 23 or 24, characterized in that: The second functional component includes a fourth bearing member and a mixing member, wherein the fourth bearing member is used to bear the nucleic acid extraction container to perform an incubation action, and the mixing member is used to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container on the fourth bearing member; Wherein, the mixing component comprises at least a first magnet array and a second magnet array extending horizontally, and each of the magnet arrays comprises a plurality of magnets installed at intervals on a magnet bracket; The fourth bearing member comprises a container receiving portion for placing the nucleic acid extraction container; The fourth bearing member and the mixing member are arranged relative to each other so that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array; The fourth bearing member and the mixing member can move horizontally relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion; The fourth supporting member and the mixing member are further arranged relative to each other so that when the fourth supporting member and the mixing member move horizontally relative to each other, at least two magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

26. The sample analyzer according to claim 23 or 24, characterized in that: The second functional component includes a fourth bearing member, a mixing member and a third driving member, the fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action, the mixing member is used to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth bearing member, the mixing member includes a movable magnet bracket and a plurality of magnets fixed on the magnet bracket, and the third driving member is used to drive the magnet bracket to move, so as to drive the plurality of magnets fixed on the magnet bracket to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the moving magnets; or, The second functional component includes a fourth bearing member, a mixing member and a third driving member, wherein the fourth bearing member is used to bear the nucleic acid extraction container to perform an incubation action, the mixing member is used to generate a magnetic field to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container on the fourth bearing member, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container can perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or, The second functional component includes a fourth bearing member, a mixing member and a third driving member. The fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action. The fourth bearing member includes at least a lysis zone. The nucleic acid extraction container undergoes a lysis and capture link in the lysis zone. In the lysis and capture link, cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field that at least covers the lysis zone to at least mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container located in the lysis zone. The third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container located in the lysis zone move so that the magnetic beads capture the released nucleic acids.

27. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container; A nucleic acid extraction device, the nucleic acid extraction device is used to extract nucleic acid from the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extraction solution; A liquid transfer device, the liquid transfer device is used to transfer the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container; an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device, the detection device is used to detect the liquid to be tested; Among them, the nucleic acid extraction device has at least two workstations, each of which is used to carry the nucleic acid extraction container to perform a processing action to extract nucleic acid, and at least two of the workstations are distributed up and down in the vertical direction, and the processing action includes at least one of incubation action, mixing action, magnetic adsorption action, liquid aspiration action, and liquid injection action.

28. The sample analyzer according to claim 27, wherein: The nucleic acid extraction device extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least a sample and a reagent and performing treatment after lysing.

29. The sample analyzer according to claim 27 or 28, characterized in that: The nucleic acid extraction device comprises a first space and a second space, wherein the first space is arranged above or below the second space; The first layer of space is provided with a liquid suction and injection station, a single liquid suction station and a single liquid injection station; The second layer of space is provided with a lysis incubation station, a cleaning incubation station, a drying incubation station and an elution incubation station; Wherein, the lysis and incubation station is used to carry the nucleic acid extraction container to perform lysis and incubation actions; The cleaning and incubation station is used to carry the nucleic acid extraction container to perform cleaning and incubation actions; The drying and incubation station is used to carry the nucleic acid extraction container to perform a drying and incubation action; The elution and incubation station is used to carry the nucleic acid extraction container to perform elution and incubation actions; The aspiration and injection station is used to carry the nucleic acid extraction container to perform a first aspiration action and a first injection action of injecting a cleaning solution; The single liquid aspiration station is used to carry the nucleic acid extraction container to perform a second liquid aspiration action; The single liquid injection station is used to carry the nucleic acid extraction container to perform a second liquid injection action of injecting an elution reagent.

30. A sample analyzer, characterized in that: include: A sample dispensing device, the sample dispensing device is used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container; A nucleic acid extraction device, the nucleic acid extraction device is used to perform a nucleic acid extraction operation on the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extraction solution; A liquid transfer device, the liquid transfer device is used to transfer the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container; an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device, the detection device is used to detect the liquid to be tested; In which, the nucleic acid extraction device includes a lysis module, a cleaning module, an elution module and a transfer component, at least two of the lysis module, the cleaning module and the elution module are distributed in the vertical direction, the lysis module is used to carry the nucleic acid extraction container for lysis operation; the cleaning module is used to carry the nucleic acid extraction container for cleaning operation; the elution module is used to carry the nucleic acid extraction container for elution operation; the transfer component is used to transfer the nucleic acid extraction container to the lysis module, the cleaning module and the elution module in sequence.

31. The sample analyzer of claim 30, wherein: The nucleic acid extraction device extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least a sample and a reagent and performing treatment after lysing.

32. The sample analyzer according to claim 30 or 31, characterized in that: The nucleic acid extraction device also includes a drying module, which is arranged below or above at least one of the lysis module, the cleaning module, and the elution module. The drying module is used to carry the nucleic acid extraction container for drying operations, and the transfer component is used to transfer the nucleic acid extraction container to the lysis module, the cleaning module, the drying module, and the elution module in sequence.

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