Cultivation method of long fruit ginger

Abstract

The invention relates to the technical field of plant cultivation. The invention provides a method for cultivating long fruit ginger. The method comprises the following steps: pre-treating long fruit ginger seeds; placing the pretreated long fruit ginger seeds in a culture medium containing specific hormones for culturing to obtain germinated long fruit ginger seeds; the specific hormones comprise GA, IAA and 6-BA; transferring the germinated long fruit ginger seeds into a culture medium for seedling strengthening and rooting; when the seedlings of the long fruit ginger reach a certain growth stage, seedling hardening treatment is carried out, and then the seedlings are transplanted to soil or other suitable growth media. According to the method for cultivating the zingiber longfruit, the germination rate and the germination potential of the zingiber longfruit seeds can be increased, the germination time lag of the zingiber longfruit can be reduced, and the survival rate of seedlings is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cultivation, and particularly relates to a cultivation method for Globba lanceolata. Background Art

[0002] As a perennial herb of the genus Globba in the family Zingiberaceae, Globba lanceolata is mainly distributed in multiple counties (cities) of Yunnan Province. Its habitat is special and the distribution range is narrow. Due to the destruction of the habitat and the scarcity of the population, the existing population of Globba lanceolata is extremely threatened with survival. It has been listed as a national Class II key protected wild plant, as well as an endangered species in the China Biodiversity Red List and the List of Threatened Species of Higher Plants in China. In recent years, Globba lanceolata has been included in the list of wild plants with extremely small populations in Yunnan Province, and the protection of its germplasm resources is extremely urgent.

[0003] However, there are many difficulties in the natural reproduction of Globba lanceolata. It grows under dense forests with a long fruiting period. The fruits are easily eaten by animals, resulting in a low seed setting rate. At the same time, the germination rate of the seeds of Globba lanceolata is also relatively low, there are few seedlings in the population, and natural regeneration is difficult. These factors seriously restrict the restoration and sustainable development of the Globba lanceolata population.

[0004] At present, no specific methods and techniques for the germination of Globba lanceolata seeds have been reported. Summary of the Invention

[0005] The present invention provides a cultivation method for Globba lanceolata to improve the germination rate, germination potential of Globba lanceolata seeds, reduce the germination lag period of Globba lanceolata, and improve the survival rate of seedlings.

[0006] A cultivation method for Globba lanceolata includes: Performing pretreatment on Globba lanceolata seeds; Placing the pretreated Globba lanceolata seeds in a culture medium containing specific hormones for cultivation to obtain germinated Globba lanceolata seeds; the specific hormones include: gibberellin GA, indoleacetic acid IAA, 6-benzylaminopurine 6-BA; Transferring the germinated Globba lanceolata seeds to a culture medium for strong seedling rooting; When the Globba lanceolata seedlings reach a certain growth stage, performing acclimatization treatment, and then transplanting the seedlings into a growth medium.

[0007] Further, in the cultivation method for Globba lanceolata as described above, the pretreatment includes: Selecting Globba lanceolata seeds that are uniform in size, plump, without damage, and without disease spots, soaking them in warm water at 50°C for 30 min, then disinfecting them with 0.1% mercuric chloride for 15 min, and rinsing them 5-6 times with sterile distilled water.

[0008] Further, for the cultivation method of Siliquamomum tonkinense described above, the cultivation of the pre-treated Siliquamomum tonkinense seeds in a culture medium containing specific hormones includes: Soaking the pre-treated Siliquamomum tonkinense seeds in GA, IAA, and 6-BA first; After the soaking, rinsing the seeds with pure water 3 - 5 times; Germinating the rinsed seeds under the conditions of a light duration of 12 h / d, a light intensity of 20%, a temperature of 25 °C, and a relative humidity (RH) of 80% until the radicle breaks through the seed coat by 2 mm and the Siliquamomum tonkinense seedlings grow to 4 - 7 cm.

[0009] Further, for the cultivation method of Siliquamomum tonkinense described above, the strong seedling rooting includes: Transferring the Siliquamomum tonkinense seedlings that have grown to 4 - 7 cm to a culture medium for cultivation; The culture medium is: MS + 30 g / L of sucrose + 6 g / L of agar + 0.5 mg / L of 6-BA + 1.5 mg / L of NAA, with a pH of 5.8; The temperature in the tissue culture room is 25 °C, and the light duration is 10 h.

[0010] Further, for the cultivation method of Siliquamomum tonkinense described above, the acclimatization treatment includes: After the plants take root, placing the tissue culture bottle seedlings with more than 3 leaves, more than 6 roots, and a root length of more than 2 cm in the greenhouse for acclimatization for 5 - 7 d and then transplanting them in the greenhouse.

[0011] Further, for the cultivation method of Siliquamomum tonkinense described above, the transplanting substrate used for the transplanting speed is peat mixed with perlite, and the volume ratio of peat to perlite is 2:1.

[0012] The cultivation method of Siliquamomum tonkinense provided by the present invention can not only improve the germination rate and germination potential of Siliquamomum tonkinense seeds, but also reduce the germination lag time of Siliquamomum tonkinense and improve the survival rate of seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a schematic diagram of the germination results of Siliquamomum tonkinense seeds under different hormone concentration conditions; Figure 2 It is a curve graph of the effects of the hormone GA on the germination rate, germination potential, and germination index of Siliquamomum tonkinense seeds; among them, GP is the germination rate, GE is the germination potential, and GI is the germination index; Figure 3 It is a curve graph of the effects of the hormone IAA on the germination rate, germination potential, and germination index of Siliquamomum tonkinense seeds; among them, GP is the germination rate, GE is the germination potential, and GI is the germination index; Figure 4The graph shows the effects of the hormone 6-BA on the germination rate, germination potential, and germination index of the seeds of *Zingiber oblongatum*; among them, GP represents the germination rate, GE represents the germination potential, and GI represents the germination index. Figure 5 The figure shows the schematic diagram of the germination process of the seeds of *Zingiber oblongatum*. Among them, a is the schematic diagram of the seeds of *Zingiber oblongatum* soaked with hormones; b is the schematic diagram of the germinated seedlings of *Zingiber oblongatum*, and c and d are the schematic diagrams of the seedlings of *Zingiber oblongatum* transferred to the culture medium. Detailed implementation methods

[0014] To make the objectives, technical solutions, and advantages of the present invention clearer, the technical solutions in the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the present invention. Obviously, the described embodiments are some, rather than all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0015] The objective of the present invention is to provide a cultivation method for *Zingiber oblongatum*. This method can improve the germination rate of seeds and the survival rate of seedlings through hormone treatment, providing technical support for the protection of germplasm resources and population restoration of *Zingiber oblongatum*. Embodiment

[0016] The cultivation method for *Zingiber oblongatum* provided by the embodiment of the present invention includes the following measures: Pretreatment of the seeds of *Zingiber oblongatum*, promoting the germination of the seeds of *Zingiber oblongatum* with hormones, strengthening seedlings and rooting in the culture medium, and acclimatizing and transplanting seedlings.

[0017] Among them, the pretreatment of the seeds of *Zingiber oblongatum* specifically includes the following measures: Select seeds that are uniform in size, plump, without damage, and without disease spots. Soak them in warm water at 50 °C for 30 minutes, then disinfect them with 0.1% mercuric chloride for 15 minutes, and rinse them 5-6 times with sterile distilled water.

[0018] Promoting the germination of the seeds of *Zingiber oblongatum* with hormones specifically includes the following measures: Germinate the pretreated seeds with gibberellin (GA), indoleacetic acid (IAA), and 6-benzylaminopurine (6-BA).

[0019] Soak the pretreated seeds in the above solution for 24 hours. The content of the soaking solution is 25 mL. After the soaking ends, rinse the seeds 3-5 times with pure water, and then transfer the seeds into a tissue culture bottle lined with sterile paper. In an artificial climate chamber (in a constant temperature and light tissue culture room), carry out germination culture under the conditions of a light time of 12 h / d, a light intensity of 20%, a temperature of 25 °C, and a relative humidity (RH) of 80% until the radicle breaks through the seed coat by 2 mm.

[0020] Strengthening seedlings and rooting in the culture medium specifically includes the following measures: When the seedlings grow to 4 - 7 cm, transfer the seedlings of Zingiber oblongatum to the medium of MS + 30 g / L sucrose + 6 g / L agar + 0.5 mg / L 6 - BA + 1.5 mg / L NAA, with a pH of 5.8. Carry out seedling strengthening and rooting under the conditions that the temperature in the tissue culture room is 25°C and the light time is 10 h.

[0021] Hardening off and transplanting specifically include the following measures: After the plants take root, place the tissue culture bottle seedlings with 3 or more leaves, more than 6 roots, and root length more than 2 cm in the greenhouse for hardening off for 5 - 7 d and then transplant them in the greenhouse. The transplanting substrate is peat mixed with perlite, and the volume ratio of peat to perlite is 2:1.

[0022] Experimental examples: Seed pretreatment: Select seeds with uniform size, plumpness, no damage, and no disease spots. Soak them in warm water at 50°C for 30 min, then disinfect them with 0.1% mercuric chloride for 15 min, and rinse them 5 - 6 times with sterile distilled water. Germination experiment:

[0023] Set 5 concentration gradients of gibberellin (GA), indole - 3 - acetic acid (IAA), and 6 - benzylaminopurine (6 - BA) for the treated seeds, which are 0 (CK sterile water), 10 mg / L, 20 mg / L, 40 mg / L, and 80 mg / L respectively. Soak the pretreated seeds in the above solutions for 24 h, with the content of the soaking solution being 25 mL. After the soaking, rinse the seeds 3 - 5 times with pure water, and then transfer the seeds into tissue culture bottles lined with sterile paper, with 3 seeds in each bottle, and set 3 treatment groups for each variable. Conduct the germination experiment in an artificial climate chamber (constant temperature and light tissue culture room) under the conditions of a light time of 12 h / d, light intensity of 20%, temperature of 25°C, and relative humidity (RH) of 80%. During the experiment, supplement sterile water according to the water loss in the culture dish to keep the sterile paper moist. Seedling strengthening and rooting in the medium:

[0024] When the seedlings grow to 4 - 7 cm, transfer the seedlings of Zingiber oblongatum to the medium of MS + 30 g / L sucrose + 6 g / L agar + 0.5 mg / L 6 - BA + 1.5 mg / L NAA, with a pH of 5.8. Carry out seedling strengthening and rooting under the conditions that the temperature in the tissue culture room is 25°C and the light time is 10 h. The results are as Figures 1-4 shown: 1. Effects of three exogenous hormones on the germination rate, germination potential, and germination index of Zingiber oblongatum GA has a certain promoting effect on the germination of Siliquamomum tonkinense seeds. In the early stage, as the concentration increases, the germination rate of Siliquamomum tonkinense increases, rising from 43% in the sterile water state to 55.55%. At a concentration of 40 mg / L, the germination rate of Siliquamomum tonkinense increases significantly. Its germination potential first gradually increases and then decreases as the GA concentration increases, reaching the maximum value at 40 ml / L. The germination index first increases and then decreases as the GA concentration increases, reaching the maximum value at 40 mg / L. Moreover, under the intervention of GA, the germination rate, germination potential, and germination index of Siliquamomum tonkinense seeds are all greater than those of Siliquamomum tonkinense seeds germinated under sterile water conditions.

[0025] IAA has a significant promoting effect on the germination of Siliquamomum tonkinense seeds. After a concentration of 10 mg / L, the germination rate of Siliquamomum tonkinense increases, rising from 43% in the sterile water state to 88% at 40 mg / L. However, as the IAA concentration continues to increase, the germination rate, germination potential, and germination index of Siliquamomum tonkinense all begin to decline, even falling below the natural germination rate of the control group. At a concentration of 40 mg / L, the germination rate, germination potential, and germination index of Siliquamomum tonkinense reach the maximum values. Generally speaking, the promoting effect of IAA is still obvious as long as the concentration gradient is well controlled.

[0026] 6-BA has no significant promoting effect on the germination rate and germination potential of Siliquamomum tonkinense, but at 20 mg / L, the germination index, germination rate, and germination potential reach the highest values, and all gradually decrease as the concentration increases, and the difference in each index compared with the sterile water condition is not obvious.

[0027] The experimental results show that the three hormones all have certain effects on the germination of Siliquamomum tonkinense seeds, but to different degrees. GA has a certain promoting effect on the germination of Siliquamomum tonkinense seeds. In the early stage, as the concentration increases, the germination rate, germination potential, and germination index of Siliquamomum tonkinense all increase. IAA has a significant promoting effect on the germination of Siliquamomum tonkinense seeds. At a concentration of 40 mg / L, the germination rate of Siliquamomum tonkinense can be increased from 43% under natural conditions to 88%. 6-BA has no obvious promoting effect on Siliquamomum tonkinense seeds, and even inhibits the germination of Siliquamomum tonkinense seeds, and the difference in each germination parameter compared with the control condition is not obvious.

[0028] 2. Effects of Three Exogenous Hormones on the Average Germination Time and Germination Lag of Siliquamomum tonkinense All three hormones can shorten the germination time of Siliquamomum tonkinense seeds to a certain extent, and can improve seed vigor, reduce the germination lag, and shorten the germination time at specific concentrations. Figure 1It can be seen that when GA is at 20 mg / L, the average germination time of Siliquamomum tonkinense is shortened from 74.33 days in the control experiment to 60.75 days, and the germination lag is shortened from 89 days to 59 days. Subsequently, as the GA concentration increases, the average germination time of Siliquamomum tonkinense gradually becomes longer. The germination time of Siliquamomum tonkinense seeds shows a trend of first decreasing and then increasing as the IAA concentration increases. When IAA is at 40 mg / L, the average germination time of Siliquamomum tonkinense is shortened to 60.50 days. Under the influence of the 6-BA hormone, the average germination time of Siliquamomum tonkinense seeds first shows a downward trend as the hormone concentration increases. When the concentration reaches 800 mg / L, the germination time of Siliquamomum tonkinense seeds increases from 61.67 days at 40 mg / L to 78.25 days.

[0029] Among them, Figure 2 is a double-coordinate data statistical chart, GP is the germination rate, and when the abscissa is 0, it represents that the germination rate when the hormone concentration is 0 is 43.3%.

[0030] As Figure 5 shown, it can be seen from Figure 5 that by using the method of the present invention, the germination rate of seeds and the survival rate of seedlings can be improved.

[0031] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some of the technical features. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cultivation method of Zingiber oblongatum, characterized in that, Including: Pre-treating the seeds of Siliquamomum tonkinense; Culturing the pre-treated seeds of Siliquamomum tonkinense in a culture medium containing specific hormones to obtain germinated seeds of Siliquamomum tonkinense; The specific hormones include: GA, IAA, 6-BA; Transferring the germinated seeds of Siliquamomum tonkinense to a culture medium for strong seedling growth and rooting; After the seedlings of Siliquamomum tonkinense take root, performing acclimatization treatment, and then transplanting the seedlings to a growth medium.

2. The cultivation method of Siliquamomum tonkinense according to claim 1, characterized in that The pre-treatment includes: Selecting seeds of Siliquamomum tonkinense with uniform size, plumpness, no damage, and no disease spots, soaking them in warm water at 50 °C for 30 min, then disinfecting them with 0.1% mercuric chloride for 15 min, and rinsing them 5-6 times with sterile distilled water.

3. The cultivation method of Siliquamomum tonkinense according to claim 1, characterized in that, The culturing of the pre-treated seeds of Siliquamomum tonkinense in a culture medium containing specific hormones includes: Soaking the pre-treated seeds of Siliquamomum tonkinense in GA, IAA, and 6-BA first; After the soaking, rinsing the seeds 3-5 times with pure water; Germinating the rinsed seeds under the conditions of a light duration of 12 h / d, a light intensity of 20%, a temperature of 25 °C, and a relative humidity of 80% until the radicle breaks through the seed coat by 2 mm and the seedlings of Siliquamomum tonkinense grow to 4-7 cm.

4. The cultivation method of Siliquamomum tonkinense according to claim 1, characterized in that, The strong seedling growth and rooting include: Transferring the seedlings of Siliquamomum tonkinense that have grown to 4-7 cm to a culture medium for culturing; The culture medium is: MS + 30 g / L of sucrose + 6 g / L of agar + 0.5 mg / L of 6-BA + 1.5 mg / L of NAA, and the pH is 5.8; The temperature in the tissue culture room is 25 °C, and the light duration is 10 h.

5. The cultivation method of Siliquamomum tonkinense according to claim 1, characterized in that, The acclimatization treatment includes: After the plants take root, placing the tissue culture bottle seedlings with more than 3 leaves, more than 6 roots, and a root length of more than 2 cm in the greenhouse for acclimatization for 5-7 d and then transplanting them in the greenhouse.

6. The cultivation method of Siliquamomum tonkinense according to claim 5, characterized in that, The transplanting substrate used for the transplanting speed is peat mixed with perlite, and the volume ratio of peat to perlite is 2:1.

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