Ginseng, cape jasmine and sunflower disc composition, ginseng, cape jasmine and sunflower disc slices, and preparation method and application of ginseng, cape jasmine and sunflower disc composition

Abstract

The invention discloses a ginseng, cape jasmine and sunflower disc composition, a ginseng, cape jasmine and sunflower disc slice and a preparation method and application of the ginseng, cape jasmine and sunflower disc composition, and belongs to the technical field of functional food. In order to solve the problems of large side effects, complex components and the like of the existing gout treatment medicines, the invention optimizes the preparation methods of a sunflower disc extract, a gardenia extract, a ginseng extract, a corn stigma extract, a dandelion extract and a cichorium intybus extract through the methods of extraction, enzymolysis, molecular weight interception and the like. The obtained extract is rich in active sugar compounds with small molecular weights, flavones, alkaloids and other effective components. The invention also provides a health-care product prepared from 80-120 parts by weight of sunflower disc extract, 5-15 parts by weight of gardenia extract, 15-30 parts by weight of ginseng extract, 6-10 parts by weight of corn stigma extract, 6-10 parts by weight of dandelion extract and 5-15 parts by weight of cichorium intybus extract. The invention discloses a composition composed of 80-100 parts by weight of microcrystalline cellulose, 1-2 parts by weight of magnesium stearate and 1-2 parts by weight of sorbitol, and a preparation method thereof. The composition can be used for development of health care products and medicines for improving gout and / or hyperuricemia.

Description

Technical Field

[0001] The present invention belongs to the technical field of functional foods, and particularly relates to a ginseng, gardenia and sunflower disc composition, ginseng, gardenia and sunflower disc tablets, a preparation method and application of the ginseng, gardenia and sunflower disc composition. Background Art

[0002] Gout is a crystal-related arthropathy caused by the deposition of monosodium urate (MSU), which is directly related to hyperuricemia caused by purine metabolism disorder and / or reduced uric acid excretion. It mainly includes acute episodic arthritis, tophus formation, tophaceous chronic arthritis, urate nephropathy and uric acid urinary calculi. In severe cases, joint disability and renal insufficiency may occur. Gout is often accompanied by abdominal obesity, hyperlipidemia, hypertension, type 2 diabetes and cardiovascular diseases.

[0003] In the acute attack period, gout and hyperuricemia can be treated or alleviated by drugs. However, if no comprehensive prevention and treatment measures are taken after the symptoms in the acute period are controlled, it is very difficult to return to normal automatically. At present, chemical drugs are mostly used to treat acute gout or hyperuricemia. Although they have certain effects, they have large side effects and cause very serious irritation to the gastrointestinal tract. Patients often experience diarrhea, nausea, acid reflux and other phenomena after taking the drugs, which seriously affect the quality of life of patients. Some health care drugs claim to have the effects of alleviating gout and reducing uric acid levels, but their compositions are too complex. Some even have more than 10 ingredients in the formula, and it is difficult to control the quality standards during production, and the active ingredients are not clear enough. And many of them have not been confirmed by animal experiments or human trials. Summary of the Invention

[0004] In order to solve the problems of large side effects and complex components of existing gout treatment drugs, the present invention provides a ginseng, gardenia and sunflower disc composition, which is composed of the following raw materials in parts by weight: 80-120 parts by weight of sunflower disc extract, 5-15 parts by weight of gardenia extract, 15-30 parts by weight of ginseng extract, 6-10 parts by weight of corn silk extract, 6-10 parts by weight of dandelion extract, 5-15 parts by weight of chicory extract, 80-100 parts by weight of microcrystalline cellulose, 1-2 parts by weight of magnesium stearate, and 1-2 parts by weight of sorbitol.

[0005] Preferably, the composition is composed of the following raw materials in parts by weight: 100 parts of sunflower disc extract, 10 parts of gardenia extract, 20 parts of ginseng extract, 8 parts of corn silk extract, 8 parts of dandelion extract, 10 parts of chicory extract, 100 parts of microcrystalline cellulose, 2 parts of magnesium stearate, and 2 parts of sorbitol.

[0006] The present invention also provides a preparation method of the above-mentioned ginseng, gardenia and sunflower disc composition, which includes the following steps:

[0007] 1) Preparation of sunflower disc extract:

[0008] a. Crush the sunflower heads, add ethanol for ethanol extraction, filter after extraction, collect the ethanol extract, add water to the residue for water extraction, filter and collect the water extract, combine the ethanol extract and the water extract, and centrifuge the obtained extraction mixture after concentration to obtain the supernatant;

[0009] b. Enzymatically hydrolyze the supernatant with a compound enzyme preparation, and the enzymatic hydrolysate is successively filtered and dried to obtain the sunflower head extract;

[0010] 2) Preparation of gardenia extract: Take dry gardenia fruits, add ethanol for ethanol extraction, remove the ethanol after extraction, and spray dry to obtain the gardenia extract;

[0011] 3) Preparation of ginseng extract: Take dry ginseng rhizomes and cut them into pieces, add ethanol for ethanol extraction, repeat the extraction 2 times, combine the 3 filtrates and then remove the ethanol, enzymatically hydrolyze the extract with a compound enzyme preparation, and the enzymatic hydrolysate is successively filtered and dried to obtain the ginseng extract.

[0012] 4) Preparation of corn silk extract: Take dry corn silk, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, concentrate the obtained extraction mixture and then centrifuge, and spray dry the obtained supernatant to obtain the corn silk extract.

[0013] 5) Preparation of dandelion extract: Take the whole dandelion herb, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, cool the obtained extraction mixture to 37 °C, add amylase for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray dry the obtained supernatant to obtain the dandelion extract.

[0014] 6) Preparation of chicory extract: Take fresh chicory roots and cut them into pieces, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, add lactase to the obtained extraction mixture for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray dry the obtained supernatant to obtain the corn silk extract.

[0015] Mix the above-mentioned sunflower head extract, gardenia extract, ginseng extract, corn silk extract, dandelion extract, chicory extract with microcrystalline cellulose, magnesium stearate, and sorbitol according to parts by weight to obtain the ginseng-gardenia-sunflower head composition.

[0016] Further defined, the ethanol extraction in step 1) means adding an ethanol solution with a volume fraction of 50% to the sunflower head powder according to a material-liquid ratio of 1 kg: 5 L, and refluxing and extracting at 55 - 62 °C for 1.5 - 2.5 h; the water extraction means adding water to the residue according to a ratio of 1 kg of raw material plus 8 L of water, and refluxing and extracting at 55 - 62 °C for 1.5 - 2.5 h.

[0017] Further limitation: The enzymatic hydrolysis in step 1) means adding a complex enzyme preparation to the supernatant according to the ratio of 3 g of the complex enzyme preparation per 1 L of the concentrated solution, reacting at 37°C for 60 min; the complex enzyme preparation is composed of α-glucosidase, β-galactosidase, and α-arabinofuranosidase, and the mass ratio of each enzyme is: α-glucosidase:β-galactosidase:α-arabinofuranosidase = 2:1:1. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0018] Further limitation: The alcohol extraction in step 2)6. means adding an ethanol solution with a volume fraction of 60% to gardenia according to the material-liquid ratio of 1 kg:(3 - 7) L, refluxing and extracting at 50°C for 2 h, removing ethanol, and drying.

[0019] Further limitation: The alcohol extraction in step 3) means adding an ethanol solution with a volume fraction of 30% to the cut ginseng rhizome according to the material-liquid ratio of 1 kg:(6 - 10) L, refluxing and extracting at 60°C for 2 h, repeating the extraction 2 times, combining the 3 filtrates and removing ethanol. The enzymatic hydrolysis means adding a complex enzyme preparation to the extract and reacting at 37°C for 60 min; the complex enzyme preparation is composed of endo-inulinase and amylase, and the mass ratio of each enzyme is: endo-inulinase:amylase = 3:1. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0020] Further limitation: The water extraction in step 4) means adding drinking water to corn silk according to the material-liquid ratio of 1 kg:8 L, extracting at 100°C for 2 h, repeating the extraction 1 time, combining the 2 filtrates, concentrating at 60°C and then centrifuging (4500 rpm, 10 min), and drying.

[0021] Further limitation: The water extraction in step 5) means adding drinking water to dandelion according to the material-liquid ratio of 1 kg:8 L, extracting at 100°C for 2 h, repeating the extraction 1 time, and combining the 2 filtrates. The enzymatic hydrolysis means adding amylase to the filtrate cooled to 37°C according to the ratio of 3 g of amylase per 1 L of the concentrated solution, reacting at 37°C for 25 min, then concentrating at 60°C and centrifuging (4500 rpm, 10 min), and drying the obtained supernatant. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0022] Further limitation: The water extraction in step 6) means adding drinking water to chicory according to the material-liquid ratio of 1 kg:6 L, extracting at 100°C for 2 h, repeating the extraction 1 time, and combining the 2 filtrates. The enzymatic hydrolysis means adding lactase to the extract according to the ratio of 3 g of lactase per 1 L of the concentrated solution, reacting at 42°C for 30 min, then concentrating at 60°C and centrifuging (4500 rpm, 10 min), and drying the obtained supernatant. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

[0023] Further defined, the filtration in step 1) means first filtering the enzymatic hydrolysate through a 100-mesh gauze and then through a microporous membrane with a pore size of 4 μm; the filtration in step 3) means filtering the enzymatic hydrolysate through nanofiltration; the filtration in steps 6) and 7) is a membrane filtration fractionation method, intercepting components with a molecular weight cut-off of 100 - 5000 Da; the drying in steps 1) - 6) is all spray drying.

[0024] The present invention also provides a ginseng, gardenia, and sunflower disk tablet, containing the above-mentioned ginseng, gardenia, and sunflower disk composition.

[0025] The ginseng, gardenia, and sunflower disk composition described in the present invention can be used to prepare health products or drugs for relieving or treating gout and hyperuricemia.

[0026] Beneficial effects

[0027] The present invention provides a ginseng, gardenia, and sunflower disk composition with a relatively simple composition, clear active ingredients, proven functions through animal experiments, and no side effects.

[0028] During the preparation process of the ginseng, gardenia, and sunflower disk composition described in the present invention, low temperatures of about 60 °C are used in both extraction and concentration, which can effectively prevent the destruction of the structures and textural properties of carbohydrates and polyphenols in the water extract by high temperatures.

[0029] Mouse experiments show that the ginseng, gardenia, and sunflower disk composition prepared by the present invention can effectively relieve gout symptoms, significantly reduce the blood uric acid level in hyperuricemic mice, and can be used for the development of drugs for relieving gout and reducing hyperuricemia.

[0030] The present invention has established a preparation method for extracts of ginseng, gardenia, sunflower disk, gardenia, ginseng, corn silk, dandelion, and chicory, and provides a tablet mainly composed of the above six raw materials for treating gout and hyperuricemia, and no identical products are seen on the market. Specific embodiments

[0031] Example 1. Preparation of the ginseng, gardenia, and sunflower disk composition.

[0032] The ginseng, gardenia, and sunflower disk composition described in this example is composed of the following raw materials in parts by weight: 100 parts of sunflower disk extract, 10 parts of gardenia extract, 20 parts of ginseng extract, 8 parts of corn silk extract, 8 parts of dandelion extract, 10 parts of chicory extract, 100 parts of microcrystalline cellulose, 2 parts of magnesium stearate, and 2 parts of sorbitol. The preparation method is as follows:

[0033] 1) Preparation of sunflower disk extract:

[0034] a. Crush the sunflower head, add ethanol for ethanol extraction, filter after extraction, collect the ethanol extract, add water to the residue for water extraction, filter and collect the water extract, combine the ethanol extract and the water extract, and centrifuge the obtained extraction mixture after concentration to obtain the supernatant;

[0035] b. Enzymatically hydrolyze the supernatant with a composite enzyme preparation, and sequentially filter and dry the enzymatic hydrolysate to obtain the sunflower head extract;

[0036] 2) Preparation of gardenia extract: Take dry gardenia fruits, add ethanol for ethanol extraction, remove ethanol after extraction, and obtain the gardenia extract after spray drying;

[0037] 3) Preparation of ginseng extract: Take dry ginseng rhizome and cut it into pieces, add ethanol for ethanol extraction, repeat the extraction 2 times, combine the filtrates of 3 extractions and then remove ethanol, enzymatically hydrolyze the extract with a composite enzyme preparation, and sequentially filter and dry the enzymatic hydrolysate to obtain the ginseng extract.

[0038] 4) Preparation of corn silk extract: Take dry corn silk, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the filtrates of 2 extractions, centrifuge the obtained extraction mixture after concentration, and spray dry the obtained supernatant to obtain the corn silk extract.

[0039] 5) Preparation of dandelion extract: Take the whole dandelion herb, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the filtrates of 2 extractions, cool the obtained extraction mixture to 37 °C, add amylase for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray dry the obtained supernatant to obtain the dandelion extract.

[0040] 6) Preparation of chicory extract: Take fresh chicory roots and cut them into pieces, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the filtrates of 2 extractions, add lactase to the obtained extraction mixture for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray dry the obtained supernatant to obtain the corn silk extract.

[0041] Mix the above sunflower head extract, gardenia extract, ginseng extract, corn silk extract, dandelion extract, chicory extract with microcrystalline cellulose, magnesium stearate, and sorbitol according to parts by weight to prepare the ginseng-gardenia-sunflower head composition.

[0042] Example 2. Preparation of the ginseng-gardenia-sunflower head composition.

[0043] The ginseng-gardenia-sunflower head composition described in this example is composed of the following raw materials in parts by weight: 80 parts of sunflower head extract, 5 parts of gardenia extract, 15 parts of ginseng extract, 6 parts of corn silk extract, 6 parts of dandelion extract, 5 parts of chicory extract, 80 parts of microcrystalline cellulose, 1 part of magnesium stearate, and 1 part of sorbitol. The preparation method is the same as that described in Example 1.

[0044] Example 3. Preparation of ginseng, gardenia and sunflower disk composition.

[0045] The ginseng, gardenia and sunflower disk composition described in this example is composed of the following raw materials in parts by weight: 120 parts of sunflower disk extract, 15 parts of gardenia extract, 30 parts of ginseng extract, 10 parts of corn silk extract, 10 parts of dandelion extract, 15 parts of chicory extract, 100 parts of microcrystalline cellulose, 2 parts of magnesium stearate, and 2 parts of sorbitol. The preparation method is the same as that described in Example 1.

[0046] Example 4. Preparation of ginseng, gardenia and sunflower disk composition.

[0047] The ginseng, gardenia and sunflower disk composition described in this example is composed of the following raw materials in parts by weight: 100 parts of sunflower disk extract, 10 parts of gardenia extract, 20 parts of ginseng extract, 8 parts of corn silk extract, 8 parts of dandelion extract, 10 parts of chicory extract, 100 parts of microcrystalline cellulose, 2 parts of magnesium stearate, and 2 parts of sorbitol. The preparation method is as follows:

[0048] 1) Preparation of sunflower disk extract:

[0049] a. Crush the sunflower disk, add ethanol for ethanol extraction, filter after extraction, collect the ethanol extract, add water to the residue for water extraction, filter and collect the water extract, combine the ethanol extract and the water extract, and centrifuge the obtained extraction mixture after concentration to obtain the supernatant;

[0050] b. Enzymatically hydrolyze the supernatant with a composite enzyme preparation, and the enzymatic hydrolysate is sequentially filtered and dried to obtain the sunflower disk extract;

[0051] 2) Preparation of gardenia extract: Take the dried gardenia fruits, add ethanol for ethanol extraction, remove the ethanol after extraction, and obtain the gardenia extract after spray drying;

[0052] 3) Preparation of ginseng extract: Take the dried ginseng rhizome and cut it into pieces, add ethanol for ethanol extraction, repeat the extraction 2 times, combine the filtrates of the 3 extractions and then remove the ethanol, enzymatically hydrolyze the extract with a composite enzyme preparation, and the enzymatic hydrolysate is sequentially filtered and dried to obtain the ginseng extract.

[0053] 4) Preparation of corn silk extract: Take the dried corn silk, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the filtrates of the 2 extractions, centrifuge the obtained extraction mixture after concentration, and spray dry the obtained supernatant to obtain the corn silk extract.

[0054] 5) Preparation of dandelion extract: Take the whole dandelion herb, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the filtrates of the 2 extractions, cool the obtained extraction mixture to 37 °C, add amylase for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray dry the obtained supernatant to obtain the dandelion extract.

[0055] 6) Preparation of chicory extract: Cut fresh chicory roots into pieces, perform water extraction with water, filter to collect the water extract, repeat the extraction once, combine the two filtrates, add lactase to the obtained extraction mixture for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray-dry the obtained supernatant to obtain chicory extract.

[0056] Mix the above-mentioned sunflower disk extract, gardenia extract, ginseng extract, corn silk extract, dandelion extract, chicory extract with microcrystalline cellulose, magnesium stearate, and sorbitol according to parts by weight to prepare the ginseng-gardenia-sunflower disk composition.

[0057] Example 5. Preparation of the ginseng-gardenia-sunflower disk composition.

[0058] The ginseng-gardenia-sunflower disk composition described in this example is composed of the following raw materials in parts by weight: 100 parts of sunflower disk extract, 10 parts of gardenia extract, 20 parts of ginseng extract, 8 parts of corn silk extract, 8 parts of dandelion extract, 10 parts of chicory extract, 100 parts of microcrystalline cellulose, 2 parts of magnesium stearate, and 2 parts of sorbitol. The preparation method is as follows:

[0059] 1) Preparation of sunflower disk extract:

[0060] a. Crush the sunflower disk, add ethanol for ethanol extraction, filter after extraction, collect the ethanol extract, add water to the residue for water extraction, filter to collect the water extract, combine the ethanol extract and the water extract, and concentrate and centrifuge the obtained extraction mixture to obtain the supernatant;

[0061] b. Hydrolyze the supernatant with a complex enzyme preparation, and the enzymatic hydrolysate is successively filtered and dried to obtain the sunflower disk extract;

[0062] 2) Preparation of gardenia extract: Take dried gardenia fruits, add ethanol for ethanol extraction, remove the ethanol after extraction, and spray-dry to obtain the gardenia extract;

[0063] 3) Preparation of ginseng extract: Cut dried ginseng rhizomes into pieces, add ethanol for ethanol extraction, repeat the extraction twice, combine the three filtrates and then remove the ethanol, hydrolyze the extract with a complex enzyme preparation, and the enzymatic hydrolysate is successively filtered and dried to obtain the ginseng extract.

[0064] 4) Preparation of corn silk extract: Take dried corn silk, add water for water extraction, filter to collect the water extract, repeat the extraction once, combine the two filtrates, concentrate and centrifuge the obtained extraction mixture, and spray-dry the obtained supernatant to obtain the corn silk extract.

[0065] 5) Preparation of dandelion extract: Take the whole dandelion plant, perform water extraction by adding water, filter to collect the water extract, repeat the extraction once, combine the two filtrates, cool the obtained extraction mixture to 37 °C, add amylase for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray-dry the obtained supernatant to obtain dandelion extract.

[0066] 6) Preparation of chicory extract: Cut fresh chicory roots into pieces, perform water extraction by adding water, filter to collect the water extract, repeat the extraction once, combine the two filtrates, add lactase to the obtained extraction mixture for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and then centrifuge, and spray-dry the obtained supernatant to obtain corn silk extract.

[0067] Mix the above-mentioned sunflower disk extract, gardenia extract, ginseng extract, corn silk extract, dandelion extract, chicory extract with microcrystalline cellulose, magnesium stearate, and sorbitol according to weight parts to prepare the ginseng-gardenia-sunflower disk composition.

[0068] Example 6. Preparation of ginseng-gardenia-sunflower disk tablets.

[0069] The ginseng-gardenia-sunflower disk composition described in the present invention can be used to prepare tablets. For example, granulate the ginseng-gardenia-sunflower disk composition with a granulator (ZL150G granulator), press the tablets with a tablet press, and package them into tablet products of 0.8 g / tablet. Perform sensory index detection on the prepared tablet products. The detection method is as follows: Take 0.8 g of the tablet product, place it in a clean white porcelain plate, observe its color and appearance under natural light with the naked eye; immediately smell its fragrance, distinguish its taste, and check for foreign matter at the bottom of the beaker after dissolving and diluting it in a transparent glass beaker according to the usage method described on the label.

[0070] Table 1 Detection results of sensory indexes of tablet products

[0071]

[0072] Example 7. Investigate the alleviating effect of the ginseng-gardenia-sunflower disk composition on acute gout.

[0073] In this example, mice were used as the research object to investigate the alleviating effect of the ginseng-gardenia-sunflower disk composition (taking Example 1 as an example) on acute gout in mice. For convenient administration, the ginseng-gardenia-sunflower disk composition was made into tablets and given to the mice.

[0074] Experimental method: Randomly divide SD rats into a normal group, a model group, a low-dose administration group, a medium-dose administration group, a high-dose administration group, and a colchicine positive control group (0.3 mg / kg), with 6 rats in each group. The doses of the low, medium, and high administration groups of the ginseng-gardenia-sunflower disk tablets were set at 50 mg / kg, 100 mg / kg, and 200 mg / kg respectively.

[0075] The administration group and the control group were given corresponding doses of drugs by gavage, while the normal group and the model group were given the same volume of normal saline by gavage. The gavage was performed once a day at 9:00 am for 5 consecutive days. On the 6th day, an acute gouty arthritis model was established in the model group, the administration group, and the positive control group. The specific method was as follows: The injection needle containing 0.2 mL of sodium urate was held at an angle of about 45° with the back of the right ankle joint of the rats to be modeled, inserted into the medial side of the tibial tendon, and injected into the ankle joint cavity; in the normal group, 0.2 mL of PBS buffer was injected at the same position in the same way. At 0, 1, 2, 4, 6, 8, and 24 h after modeling, a total of 7 time points, the right ankle joint diameter (mm) of the experimental rats was measured, and the measurement was continuously performed 3 times to calculate the average value.

[0076] Degree of joint swelling (mm) = Ankle joint diameter (mm) after modeling - Ankle joint diameter (mm) before modeling. The gait of each group of rats was observed: Grade 0 - Normal walking (0 points); Grade 1 - Slight limping, with the tested lower limb slightly bent (1 point); Grade 2 - Moderate limping, with the tested lower limb just touching the ground (2 points); Grade 3 - Severe limping, with the tested lower limb leaving the ground and walking on three legs (3 points).

[0077] Experimental results: After modeling, as time prolonged, the swelling degree of the rats' ankle joints gradually increased, and the swelling degree was the most severe at 8 h after modeling; there was no obvious swelling in the ankle joints of the rats in the normal group, and the difference was statistically significant compared with the model group (P < 0.01), indicating that the model was successfully established. Compared with the model group, the swelling degrees of the ankle joints in the three dose groups of ginseng, gardenia, and sunflower disk tablets and the colchicine group all decreased; there was no statistically significant difference between the three dose groups of ginseng, gardenia, and sunflower disk tablets and the colchicine group. The specific results are shown in Table 2.

[0078] Table 2 Effects of ginseng, gardenia, and sunflower disk tablets on the swelling degree of ankle joints in rats with acute gouty arthritis

[0079]

[0080]

[0081] Note: Compared with the model group b p < 0.05; c p < 0.01

[0082] After 24 h of modeling, the activity of the rats in the model group decreased, the walking posture was abnormal, the tested lower limb lightly touched or left the ground, showing a gait of walking on three legs, and the joint was locally red and swollen. Compared with the model group, the gaits of the three dose groups of ginseng, gardenia, and sunflower disk tablets and the colchicine group were all significantly improved, and the difference was statistically significant. The specific results are shown in Table 3.

[0083] Table 3 Effects of Ginseng, Gardenia and Sunflower Disc Tablets on the Gait of Rats with Acute Gouty Arthritis

[0084]

[0085] Note: Compared with the model group c p < 0.01

[0086] Example 8. Investigate the alleviating effect of the ginseng, gardenia and sunflower disc composition on hyperuricemia.

[0087] In this example, mice were used as the research object to investigate the alleviating effect of the ginseng, gardenia and sunflower disc composition (taking Example 1 as an example) on hyperuricemia in mice. For convenient administration, the ginseng, gardenia and sunflower disc composition was made into tablet form and given to mice.

[0088] Experimental method: Male Balb-c mice were randomly divided into a normal group, a model group, a positive control allopurinol group (20 mg / kg), and a ginseng, gardenia and sunflower disc tablet group (0.2 g / kg), with 10 mice in each group. The administration dose of the yeast solution was 20 g / kg. From the 1st day to the 8th day, every morning, the allopurinol group and each group of ginseng, gardenia and sunflower disc tablets were given the corresponding solution by gavage; every afternoon, except for the blank group, each group was given the yeast solution by gavage. The groups that did not need gavage during the experiment were given an equal amount of normal saline. From the 5th day to the 8th day, 1 h after the yeast gavage, except for the blank group, each group was intraperitoneally injected with a 3% potassium oxonate (OXO) solution (300 g / kg). The blank group was injected with an equal amount of normal saline. 1 h after the last administration, blood was collected from the orbital venous plexus of each group of mice. After the plasma was allowed to stand for 30 min, it was centrifuged at a speed of 3000 r / min for 5 min, and the supernatant was taken and stored at -80°C. The serum uric acid content of mice was measured according to the measurement method of the kit.

[0089] Experimental results: Compared with the normal group, the serum uric acid value of the model group increased significantly, indicating that the hyperuricemia mouse model was successfully established. Compared with the model group, both allopurinol and ginseng, gardenia and sunflower disc tablets could significantly reduce the serum uric acid value of mice and were lower than the serum uric acid level of normal group mice, showing a significant effect of reducing the serum uric acid level in hyperuricemic mice. The specific results are shown in Table 4.

[0090] Table 4 Effects of Ginseng, Gardenia and Sunflower Disc Tablets on Serum Uric Acid Value in Hyperuricemic Mice

[0091]

[0092] Note: Compared with the normal group # p < 0.05; compared with the model group, *p < 0.05

[0093] In summary, the ginseng, gardenia and sunflower disk composition prepared by the present invention has a good improvement effect on gout and hyperuricemia in mice, and can be used to prepare health products or drugs with the effects of relieving or treating hyperuricemia and dissolving gouty tophi.

Claims

1. A ginseng, gardenia and sunflower disk composition, characterized in that, The composition is composed of the following raw materials in parts by weight: 80 - 120 parts by weight of sunflower disc extract, 5 - 15 parts by weight of gardenia extract, 15 - 30 parts by weight of ginseng extract, 6 - 10 parts by weight of corn silk extract, 6 - 10 parts by weight of dandelion extract, 5 - 15 parts by weight of chicory extract, 80 - 100 parts by weight of microcrystalline cellulose, 1 - 2 parts by weight of magnesium stearate, and 1 - 2 parts by weight of sorbitol.

2. The ginseng, gardenia and sunflower disk composition according to claim 1, characterized in that, The composition is composed of the following raw materials in parts by weight: 100 parts by weight of sunflower disc extract, 10 parts by weight of gardenia extract, 20 parts by weight of ginseng extract, 8 parts by weight of corn silk extract, 8 parts by weight of dandelion extract, 10 parts by weight of chicory extract, 100 parts by weight of microcrystalline cellulose, 2 parts by weight of magnesium stearate, and 2 parts by weight of sorbitol.

3. The preparation method of the ginseng, gardenia and sunflower disk composition according to claim 1 or 2, characterized in that, It includes the following steps: 1) Preparation of sunflower disc extract: a. Crush the sunflower disc, add ethanol for ethanol extraction, filter after extraction, collect the ethanol extract, add water to the residue for water extraction, filter and collect the water extract, combine the ethanol extract and the water extract, and centrifuge the obtained extraction mixture after concentration to obtain the supernatant; b. Enzymatically hydrolyze the supernatant with a complex enzyme preparation, and the enzymatic hydrolysate is sequentially filtered and dried to obtain the sunflower disc extract; 2) Preparation of gardenia extract: Take dry gardenia fruits, add ethanol for ethanol extraction, remove ethanol after extraction, and obtain the gardenia extract after spray drying; 3) Preparation of ginseng extract: Take dry ginseng rhizomes and cut them into pieces, add ethanol for ethanol extraction, repeat the extraction 2 times, remove ethanol after combining the 3 filtrates, enzymatically hydrolyze the extract with a complex enzyme preparation, and the enzymatic hydrolysate is sequentially filtered and dried to obtain the ginseng extract. 4) Preparation of corn silk extract: Take dry corn silk, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, centrifuge the obtained extraction mixture after concentration, and spray dry the obtained supernatant to obtain the corn silk extract. 5) Preparation of dandelion extract: Take the whole dandelion herb, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, cool the obtained extraction mixture to 37 °C, add amylase for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and centrifuge, and spray dry the obtained supernatant to obtain the dandelion extract. 6) Preparation of chicory extract: Take fresh chicory roots and cut them into pieces, add water for water extraction, filter and collect the water extract, repeat the extraction 1 time, combine the 2 filtrates, add lactase to the obtained extraction mixture for enzymatic hydrolysis, concentrate the enzymatic hydrolysate and centrifuge, and spray dry the obtained supernatant to obtain the corn silk extract. Mix the above sunflower disc extract, gardenia extract, ginseng extract, corn silk extract, dandelion extract, chicory extract with microcrystalline cellulose, magnesium stearate, and sorbitol according to the parts by weight to obtain the ginseng - gardenia - sunflower disc composition.

4. The preparation method according to claim 3, characterized in that, The ethanol extraction described in step 1) means adding an ethanol solution with a volume fraction of 35% to sunflower disc powder according to a material-liquid ratio of 1 kg: 3 L, and refluxing and extracting at 50 °C for 2 h; the water extraction means adding water to the residue according to a ratio of 1 kg of raw material to 10 L of water, and extracting at 60 °C for 2 h. The enzymatic hydrolysis means adding a compound enzyme preparation to the supernatant according to a ratio of 1 L of concentrated solution to 3 g of the compound enzyme preparation, and reacting at 37 °C for 60 min; the compound enzyme preparation is composed of α-glucosidase, β-galactosidase, and α-arabinofuranosidase, and the mass ratio of each enzyme is: α-glucosidase: β-galactosidase: α-arabinofuranosidase = 2:1:

1. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

5. The preparation method according to claim 3, characterized in that, The ethanol extraction described in step 2) means adding an ethanol solution with a volume fraction of 60% to gardenia according to a material-liquid ratio of 1 kg: (3 - 7) L, refluxing and extracting at 50 °C for 2 h, removing ethanol, and drying.

6. The preparation method according to claim 3, characterized in that, The ethanol extraction described in step 3) means adding an ethanol solution with a volume fraction of 30% to cut ginseng rhizome according to a material-liquid ratio of 1 kg: (6 - 10) L, refluxing and extracting at 60 °C for 2 h, repeating the extraction 2 times, and then combining the 3 filtrates and removing ethanol. The enzymatic hydrolysis means adding a compound enzyme preparation to the extract and reacting at 37 °C for 60 min; the compound enzyme preparation is composed of endoinulinase and amylase, and the mass ratio of each enzyme is: endoinulinase: amylase = 3:

1. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

7. The preparation method according to claim 3, characterized in that, The water extraction described in step 5) means adding drinking water to dandelion according to a material-liquid ratio of 1 kg: 8 L, extracting at 100 °C for 2 h, repeating the extraction 1 time, and combining the 2 filtrates. The enzymatic hydrolysis means adding amylase to the filtrate cooled to 37 °C according to a ratio of 1 L of concentrated solution to 3 g of amylase, reacting at 37 °C for 25 min, then concentrating at 60 °C and centrifuging (4500 rpm, 10 min), and drying the obtained supernatant. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

8. The preparation method according to claim 3, characterized in that, The water extraction described in step 6) means adding drinking water to chicory according to a material-liquid ratio of 1 kg: 6 L, extracting at 100 °C for 2 h, repeating the extraction 1 time, and combining the 2 filtrates. The enzymatic hydrolysis means adding lactase to the extract according to a ratio of 1 L of concentrated solution to 3 g of lactase, reacting at 42 °C for 30 min, then concentrating at 60 °C and centrifuging (4500 rpm, 10 min), and drying the obtained supernatant. All the enzymes are purchased from Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd.

9. The preparation method according to claim 3, characterized in that, The filtration described in step 1) means first filtering the enzymatic hydrolysate through a 100-mesh gauze, and then filtering through a microporous membrane with a pore size of 4 μm; the filtration described in step 3) means filtering the enzymatic hydrolysate through nanofiltration; the filtration described in steps 6) and 7) is a membrane filtration fractionation method, intercepting components with a molecular weight cut-off of 100 - 5000 Da; the drying described in steps 1) - 6) is all spray drying.

10. A ginseng, gardenia and sunflower disc tablet, characterized in that, Use of the ginseng-gardenia-sunflower disc composition according to claim 1 or 2 in the preparation of a health product or drug for relieving or treating gout and hyperuricemia.

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