Anti-hair loss composition as well as preparation method and application thereof

Abstract

The invention relates to the field of anti-hair loss products, in particular to an anti-hair loss composition as well as a preparation method and application thereof. The anti-hair loss composition comprises a first composition, a second composition, a third composition and auxiliary materials, the first composition is prepared from HEPES, adenosine tartaric acid and 1, 2-pentanediol; the second composition comprises AHK-Cu and a supramolecular solvent; the third composition is prepared from one or more of acetyl octapeptide-2, decarboxylated carnosine hydrochloride, biotin tripeptide-1 and myristoyl hexapeptide-16; the auxiliary material is a sodium hydroxide aqueous solution. Through the synergistic effect of the components, blocking is carried out from multiple dimensions, firstly, bacteriostasis and inflammation diminishing are carried out, and oil outflow is reduced; secondly, self-repairing of hair follicle cells is activated through antioxidation and generation of vascular endothelial growth factors; then, by promoting generation of collagen and keratin, hair growth is promoted; finally, the supramolecular penetration enhancer is used for improving the utilization rate of active ingredients and enhancing the effect of the composition. Therefore, through the multi-dimensional effect, alopecia is prevented, hair follicles are strengthened, and hair growth is promoted.

Description

Technical Field

[0001] The present invention relates to the field of anti - hair - loss products, and particularly to an anti - hair - loss composition, a preparation method thereof and an application thereof. Background Art

[0002] Common hair - loss manifestations include androgenetic alopecia (male - pattern alopecia or seborrheic alopecia), alopecia areata, telogen effluvium (postpartum alopecia, alopecia caused by drugs, infections, diseases, etc.). Nowadays, hair - loss is getting younger. Is it related to the above - mentioned hair - loss diseases? Hair - loss may not be caused by the above - mentioned reasons and may be related to factors such as smoking, ultraviolet rays, external pollution, mental stress, etc. For example, both mental stress and staying up late under stress may cause corticotropin - releasing hormone to stimulate hair follicles, cortisol synthesis, and the neuroendocrine feedback loop to affect the hair cycle.

[0003] These hair - loss diseases and common daily hair - loss are accompanied by oxidative stress. Oxidation - reduction reactions are constantly occurring in the human body and maintaining a certain balance. When the oxidation and antioxidant processes are unbalanced (such as diseases, microorganisms, ultraviolet rays, insufficient antioxidant supplementation, external environmental factors, long - term fatigue and exhaustion of the human body, mental factors, etc.), more oxidation will occur.

[0004] When oxygen free radicals damage the scalp cutin layer, it increases the brittleness of the cutin layer, increases the surface roughness of the hair, and the anti - tensile ability of the hair decreases, making it more prone to breakage. In addition, when oxidative stress occurs outside hair follicle melanocytes (for example, ultraviolet - induced, mental emotions, smoking, chemical substances or inflammatory stress may increase this oxidative stress), it will weaken the antioxidant ability of hair follicle melanocytes, accelerate the accumulation of damage, which means that the hair follicles begin to atrophy, the hair begins to fall out, and enters the telogen phase in advance.

[0005] The "Polypeptide Composition for Hair Growth" disclosed in Patent CN201510543484.9 is a composition composed of polypeptides with the function of promoting the generation of hair keratin and polypeptides with the function of stimulating the generation of collagen, which can be a combination of myristoyl pentapeptide-17, myristoyl hexapeptide-16, biotin tripeptide-1, acetyl tetrapeptide-3, copper peptide, etc. This patent combines polypeptides with the function of promoting the generation of hair keratin and polypeptides with the function of stimulating the generation of collagen to promote hair growth, and also enhances the generation of collagen in the skin around the hair follicles to strengthen the hair roots. Although the composition can stimulate hair follicle growth, promote the growth of new hair, supply nutrients and strengthen the roots and fix the hair, it only uses two types of polypeptide components, and the mechanism of action is relatively single. It does not consider the problems of seborrheic alopecia and pathogen infections such as Malassezia furfur and Corynebacterium acnes, which can also induce or exacerbate hair loss, nor does it consider the problem of hair loss caused by oxidative stress. This patent only considers promoting hair growth at the back end and fails to consider hair loss problems from multiple dimensions, thus failing to achieve a comprehensive anti-hair loss effect. Second, the polypeptide components are directly added to the formula. However, due to the large molecular weight of polypeptides and the existence of the skin barrier, they cannot penetrate the skin barrier well and be absorbed, reducing the utilization rate of polypeptides and making it difficult to exert the efficacy of the composition.

[0006] The "Anti-Hair Loss Composition, Preparation Method and Application" disclosed in Patent CN116617356A

[0007] It is composed of matrine salicylate, L-carnitine tartrate, polypeptides that promote collagen production, polypeptides that promote keratin production, glucosylglycerol, betaine, and 1,3-propanediol. This patent mainly uses matrine salicylate to solve the problem of scalp inflammation caused by excessive oil secretion in the scalp due to hair follicle blockage, achieving the effects of oil control, antibacterial, and anti-inflammatory, and balancing the scalp microbial environment. Then, combined with the moisturizing effect of glucosylglycerol, it synergistically improves the water-oil balance of the scalp. Then, using the repair ability of glucosylglycerol, it corrects the sub-healthy state of the scalp of hair loss patients. Finally, using L-carnitine tartrate and polypeptides that promote keratin production, it improves the activity of hair follicle cells and promotes hair growth. Although this patent can target hair loss from multiple dimensions, it mainly targets seborrheic alopecia manifested as excessive oil secretion in the scalp, extremely greasy hair, resulting in hair follicle blockage, atrophy and degeneration, and pathogen infections such as Malassezia furfur and Corynebacterium acnes, which can also induce or exacerbate hair loss. It does not propose solutions for hair loss caused by factors such as ultraviolet rays, mental stress, and oxidative stress, which overwhelm the antioxidant capacity of hair follicle melanocytes, accelerate damage accumulation, resulting in hair loss, and insufficient vascularization, reducing the supply of nutrients required by hair follicles, resulting in accelerated hair follicle atrophy.

[0008] So, with oxidative stress being so severe, how should one prevent hair loss under the influence of factors such as stress, mental factors, staying up late, and ultraviolet rays?

[0009] Therefore, developing a product that can prevent hair loss in multiple dimensions, such as for seborrheic alopecia, fungal infections like Malassezia furfur and Corynebacterium acnes, and coping with oxidative stress, and is easy to transdermally absorb, has promising prospects. Summary of the Invention

[0010] In view of the deficiencies of the above-mentioned prior art, the purpose of the present invention is to provide an anti-hair loss composition, its preparation method and application. The anti-hair loss composition can act on multiple pathways to achieve multi-dimensional hair loss prevention, aiming to solve the problem that the existing anti-hair loss products have a single mechanism of action and unsatisfactory effects.

[0011] The technical solution of the present invention is as follows:

[0012] In the first aspect, the present invention provides an anti-hair loss composition, which includes: a first composition, a second composition, a third composition, and excipients;

[0013] The first composition includes: HEPES, adenosine tartrate, 1,2-pentanediol;

[0014] The second composition includes: AHK-Cu, supramolecular solvent;

[0015] The third composition includes one or more of acetyl octapeptide-2, decarboxylated carnosine hydrochloride, biotin tripeptide-1, and myristoyl hexapeptide-16;

[0016] The excipient is an aqueous sodium hydroxide solution.

[0017] Optionally, the supramolecular solvent in the second composition is one or more of betaine propylene glycol, betaine malic acid, betaine lactic acid, betaine citric acid, L-carnitine citric acid, and L-carnitine malic acid.

[0018] Optionally, by weight, the first composition includes: 5-20 parts of HEPES, 0.1-5 parts of adenosine tartrate, and 3-20 parts of 1,2-pentanediol;

[0019] The second composition includes: 0.4-15 parts of AHK-Cu and 10-40 parts of supramolecular solvent;

[0020] The third composition includes one or more of 0.1-10 parts of acetyl octapeptide-2, 0.5-6 parts of decarboxylated carnosine hydrochloride, 0.1-10 parts of biotin tripeptide-1, and 0.1-10 parts of myristoyl hexapeptide-16.

[0021] Further optionally, by weight parts, the third composition comprises: 0.1-10 parts of acetyl octapeptide-2, 0.5-6 parts of decarboxylated carnosine hydrochloride, 0.1-10 parts of biotin tripeptide-1, and 0.1-10 parts of myristoyl hexapeptide-16.

[0022] In a second aspect, the present invention provides a method for preparing the anti-hair loss composition of the present invention, which includes the steps of: mixing the first composition, the second composition, the third composition and excipients, and stirring evenly to obtain the anti-hair loss composition.

[0023] Optionally, the method for preparing the anti-hair loss composition specifically includes the steps of:

[0024] Mixing the first composition, deionized water and excipients to obtain Solution 1;

[0025] After mixing the second composition and deionized water, adding them to Solution 1 to obtain Solution 2;

[0026] Adding the third composition to Solution 2 to obtain the anti-hair loss composition.

[0027] Further optionally, the method for preparing the anti-hair loss composition specifically includes the steps of:

[0028] Mixing the first composition with deionized water, heating, stirring evenly, then cooling to room temperature, adding excipients, and stirring evenly to obtain Solution 1;

[0029] Mixing the second composition with deionized water, stirring evenly and adding them to Solution 1, and stirring evenly to obtain Solution 2;

[0030] Adding the third composition to Solution 2, and stirring evenly to obtain the anti-hair loss composition.

[0031] Optionally, the rotation speed of stirring is 200-600 revolutions per minute;

[0032] The temperature of heating is 50-80 °C.

[0033] In a third aspect, the present invention provides an application of the anti-hair loss composition of the present invention in the preparation of anti-hair loss products.

[0034] Optionally, the anti-hair loss product is one or more of anti-hair loss shampoo, anti-hair loss hair conditioner, and anti-hair loss essence.

[0035] Beneficial effects: The present invention provides an anti - hair - loss composition, which includes: a first composition, a second composition, a third composition and excipients. The first composition includes: HEPES, adenosine tartrate, 1,2 - pentanediol; the second composition includes: AHK - Cu, supramolecular solvent; the third composition includes one or more of acetyl octapeptide - 2, decarboxylated carnosine hydrochloride, biotin tripeptide - 1, myristoyl hexapeptide - 16; the excipient is an aqueous sodium hydroxide solution. The anti - hair - loss composition of the present invention, through the compounding of each component, can target hair loss under conditions such as stress, mental factors, staying up late, etc., and intercept from multiple dimensions. First, adenosine tartrate and supramolecular solvent are used to achieve bacteriostatic and anti - inflammatory effects, reduce oil secretion, and make the hair plump and fluffy; secondly, decarboxylated carnosine hydrochloride, AHK - Cu and acetyl octapeptide - 2 are used to achieve antioxidant effects, regulate the production of vascular endothelial growth factor, and activate the self - repair of hair follicle cells, thereby strongly strengthening hair; then, myristoyl hexapeptide - 16 promotes the generation of collagen and keratin, and promotes hair growth; at the same time, supramolecular solvent and HEPES are used to improve the utilization rate of active ingredients and maintain a stable environment for the system. Therefore, through multi - dimensional effects, it can deal with seborrheic alopecia, bacterial infection, oxidative stress, prevent hair loss, strengthen hair follicles, and promote hair growth. In addition, this process solves the problem that adenosine tartrate, AHK - Cu and decarboxylated carnosine hydrochloride are unstable in the system and prone to precipitation, and maintains the stability of the system. Description of the Drawings

[0036] Figure 1 It is a diagram of Sample 1 obtained under the conditions of Comparative Example 1.

[0037] Figure 2 It is a diagram of Sample 3 obtained under the conditions of Comparative Example 3.

[0038] Figure 3 It is a diagram of the stability of the sample obtained under the conditions of Comparative Example 4.

[0039] Figure 4 It is a diagram of the cumulative permeation amount trend of biotin tripeptide - 1.

[0040] Figure 5 It is a diagram of the cumulative permeation amount trend of AHK - Cu.

[0041] Figure 6 It is a diagram of the cumulative permeation amount trend of decarboxylated carnosine hydrochloride.

[0042] Figure 7 It is a schematic diagram of the test result of the skin oil content of adenosine tartrate in Test Example 1 of the present invention.

[0043] Figure 8 It is a schematic diagram of the detection result of the relative expression amount of TGF - β2 of adenosine tartrate in Test Example 1 of the present invention.

[0044] Figure 9 Figure for the cytotoxicity detection results of anti - hair loss composition 1 based on keratinocytes.

[0045] Figure 10 Figure for the cytotoxicity detection results of anti - hair loss composition 1 based on human fibroblasts.

[0046] Figure 11 Figure for the cytotoxicity detection results of anti - hair loss composition 1 based on dermal papilla cells.

[0047] Figure 12 Schematic diagram of the IL - 6 test results of anti - hair loss composition 1 in Test Example 2 of the present invention.

[0048] Figure 13 Schematic diagram of the IL - 1β test results of anti - hair loss composition 1 in Test Example 2 of the present invention.

[0049] Figure 14 Schematic diagram of the VEGF test results of anti - hair loss composition 1 in Test Example 2 of the present invention.

[0050] Figures 12 - 14 In the figure, when performing statistical analysis using the 1 - test method, compared with the BC (blank control) group, significance is indicated by #, P - value < 0.05 is indicated by # (significant difference), and P - value < 0.01 is indicated by ## (extremely significant difference); compared with the NC (negative control) group, significance is indicated by *, P - value < 0.05 is indicated by * (significant difference), and P - value < 0.01 is indicated by ** (extremely significant difference). Detailed implementation method

[0051] The present invention provides an anti - hair loss composition, its preparation method and application. To make the purpose, technical solution and effect of the present invention clearer and more definite, the present invention is further described in detail below. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0052] An embodiment of the present invention provides an anti - hair loss composition, which includes: a first composition, a second composition, a third composition and excipients;

[0053] The first composition includes: HEPES, adenosine tartrate, 1,2 - pentanediol;

[0054] The second composition includes: AHK - Cu, supramolecular solvent;

[0055] The third composition includes one or more of acetyl octapeptide - 2, decarboxy carnosine hydrochloride, biotin tripeptide - 1, myristoyl hexapeptide - 16;

[0056] The adjuvant is an aqueous sodium hydroxide solution.

[0057] The anti - hair - loss composition described in the embodiments of the present invention, through the compounding of various components, can target hair loss under conditions such as stress, mental factors, staying up late, etc., and carry out multi - dimensional attacks. First, adenosine tartrate and supramolecular solvent are used to achieve bacteriostatic and anti - inflammatory effects, reduce oil production, and make the hair plump and fluffy; secondly, decarboxylated carnosine hydrochloride, AHK - Cu and acetyl octapeptide - 2 are used to achieve antioxidant effects, regulate the production of vascular endothelial growth factor, and activate the self - repair of hair follicle cells, thereby strongly strengthening hair; then, myristoyl hexapeptide - 16 promotes the production of collagen and keratin, promoting hair growth; at the same time, the supramolecular solvent and HEPES are used to improve the utilization rate of active ingredients and maintain a stable environment for the system. Therefore, through multi - dimensional effects, it can deal with seborrheic alopecia, bacterial infection, oxidative stress, prevent hair loss, strengthen hair follicles, and promote hair growth.

[0058] In one embodiment, by weight, the anti - hair - loss composition includes: a first composition, a second composition, a third composition, and an adjuvant;

[0059] The first composition includes: 5 - 20 parts of HEPES, 0.1 - 5 parts of adenosine tartrate, and 3 - 20 parts of 1,2 - pentanediol;

[0060] The second composition includes: 0.4 - 15 parts of AHK - Cu and 10 - 40 parts of supramolecular solvent;

[0061] The third composition includes one or more of 0.1 - 10 parts of acetyl octapeptide - 2, 0.5 - 6 parts of decarboxylated carnosine hydrochloride, 0.1 - 10 parts of biotin tripeptide - 1, and 0.1 - 10 parts of myristoyl hexapeptide - 16;

[0062] The adjuvant is 0 - 1.0 part of an 8 - 12 wt% (preferably 10 wt%) aqueous sodium hydroxide solution.

[0063] The mechanism of action of the components of the above - mentioned anti - hair - loss composition will be described below.

[0064] Adenosine tartrate (supramolecular co - crystal): Adenosine can supplement energy for skin cells to accelerate skin cell metabolism and delay aging. In hair care products for the head, adenosine can stimulate the generation of hair growth; tartaric acid can soothe the skin and regulate sebum excretion. Through the supramolecular co - crystal strengthening technology, the problems of poor solubility of adenosine in water and strong irritation of tartaric acid are solved, and at the same time, there is a synergistic effect. After testing, as Figure 1 , Table 1, Table 2, Figure 2 , adenosine tartrate has both the activities of adenosine and tartaric acid, with a synergistic effect, bacteriostatic and anti - hair - loss, and oil - control and plumping effects.

[0065] HEPES (4 - (2 - hydroxyethyl)piperazine - 1 - ethanesulfonic acid): It can loosen the stratum corneum and play a role in promoting penetration. It is also a very cell - friendly substance. Its addition can reduce skin irritation, maintain the stability of the AHK - Cu system, and act as a pH buffer.

[0066] AHK - Cu (alanine / histidine / lysine polypeptide copper HCl, where " / " represents a peptide bond) can regulate the production of vascular endothelial growth factor and promote the growth of hair follicles.

[0067] Acetyl octapeptide - 2: It can promote cell growth and migration and promote hair growth by activating hair follicle stem cells.

[0068] Decarboxy carnosine hydrochloride: It has the function of capturing hydroxyl radicals, singlet oxygen, and hydrogen peroxide radicals. It can almost completely protect proteins from cross - linking by lipid hydroperoxide and peroxidized PC - containing liposomes, and can play an inhibitory role in lipid oxidation caused by including non - metal ions.

[0069] Biotin tripeptide - 1: It can promote the synthesis of collagen IV and laminin 5, delay hair follicle aging, promote hair growth, improve the hair follicle structure, and enhance the anchoring effect of hair follicles and root sheaths on hair.

[0070] Myristoyl hexapeptide - 16: It stimulates the expression of keratin genes and promotes the production of keratin, thereby achieving the purpose of promoting hair growth.

[0071] Supramolecular solvents: Betaine propylene glycol, betaine lactate, betaine malate, betaine citrate, L - carnitine citrate, L - carnitine malate, etc. The above - mentioned several supramolecular solvents have stable structures. In addition to having a synergistic effect on promoting penetration, this series of supramolecular solvents also have the effects of soothing the scalp, balancing sebum secretion, reducing scalp inflammation. When combined with active substances, after several months of observation, the stability is relatively good. However, several supramolecular solvents such as L - hydroxyproline - rhamnose - glycerol, proline - galactose - glycerol, betaine - proline - butanediol, proline - galactose, and glycerol - xylitol not only cannot synergistically promote penetration but also make the system unstable when combined with active substances. That is to say, not all supramolecular solvents can synergistically promote penetration and maintain the stability of the system. The construction of the system of supramolecular solvents and active substances has a high degree of selectivity.

[0072] The embodiment of the present invention provides a preparation method of the above - mentioned anti - hair - loss composition, which includes the steps of: mixing the first composition, the second composition, the third composition, and auxiliary materials, and stirring evenly to obtain the anti - hair - loss composition.

[0073] In one embodiment, the preparation method of the anti - hair - loss composition specifically includes the steps of:

[0074] Mix the first composition, deionized water and auxiliary materials to obtain Solution 1;

[0075] Mix the second composition and deionized water, and then add the mixture to Solution 1 to obtain Solution 2;

[0076] Add the third composition to Solution 2 to obtain the anti-hair loss composition;

[0077] By weight, the first composition includes: 5-20 parts of HEPES, 0.1-5 parts of adenosine tartrate, and 3-20 parts of 1,2-pentanediol;

[0078] The second composition includes: 0.4-15 parts of AHK-Cu and 10-40 parts of supramolecular solvent;

[0079] The third composition includes: 0.1-10 parts of acetyl octapeptide-2, 0.5-6 parts of decarboxylated carnosine hydrochloride, 0.1-10 parts of biotin tripeptide-1, and 0.1-10 parts of myristoyl hexapeptide-16;

[0080] The auxiliary material is 0-1.0 part of 8-12wt% sodium hydroxide aqueous solution.

[0081] In one embodiment, the preparation method of the anti-hair loss composition specifically includes the steps of:

[0082] Mix the first composition and deionized water, heat up, stir evenly, then cool down to room temperature, add the auxiliary material, and stir evenly to obtain Solution 1;

[0083] Mix the second composition and deionized water, dissolve and add the mixture to Solution 1, and stir evenly to obtain Solution 2;

[0084] Add the third composition to Solution 2 and stir evenly to obtain the anti-hair loss composition.

[0085] In the examples of the present invention, the above process is adopted to solve the problems that adenosine tartrate, AHK-Cu and decarboxylated carnosine hydrochloride are unstable in the system and prone to precipitation, and maintain the stability of the system. Specifically, the added auxiliary material (i.e., sodium hydroxide aqueous solution) can adjust the pH to increase the solubility of adenosine tartrate and decarboxylated carnosine hydrochloride. Then pre-dissolve AHK-Cu, HEPES and betaine propanediol to keep AHK-Cu stable, and then mix and stir evenly with other components. This process not only solves the problem that the solution becomes turbid after adding AHK-Cu, but also solves the problem of precipitation of decarboxylated carnosine hydrochloride.

[0086] Among them, the rotation speed of the stirring is 200-600 revolutions per minute, such as 400 revolutions per minute.

[0087] Among them, the temperature for heating is 50 - 80°C, such as 60°C.

[0088] An embodiment of the present invention provides an application of the anti - hair - loss composition as described above in the preparation of anti - hair - loss products. Among them, the anti - hair - loss products can be anti - hair - loss shampoos, anti - hair - loss hair conditioners, anti - hair - loss essences, etc., but are not limited thereto.

[0089] The present invention will be further described below through several specific embodiments.

[0090] Comparative Example 1

[0091] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 55.6 parts of deionized water, heat to 60°C, stir evenly, and then cool to room temperature to obtain Solution 1; add 1 part of AHK - Cu, 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, and 0.1 part of myristoyl hexapeptide - 16 into Solution 1 respectively, stir evenly to obtain Solution 2; add 5 parts of 1,2 - pentanediol and 25 parts of betaine propanediol into Solution 2, stir evenly to obtain Comparative Composition 1 (denoted as Sample 1).

[0092] Comparative Example 2

[0093] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 45.6 parts of deionized water, heat to 60°C, stir evenly, and then cool to room temperature to obtain Solution 1; add 1 part of AHK - Cu, 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, and 0.1 part of myristoyl hexapeptide - 16 into Solution 1 respectively, stir evenly to obtain Solution 2; add 15 parts of 1,2 - pentanediol and 25 parts of betaine propanediol into Solution 2, stir evenly to obtain Comparative Composition 2 (denoted as Sample 2).

[0094] Comparative Example 3

[0095] Mix 3 parts of adenosine tartrate with 45.6 parts of deionized water and 15 parts of 1,2 - pentanediol, heat to 60°C, stir evenly, and then cool to room temperature to obtain Solution 1; add 0.5 part of AHK - Cu into Solution 1, stir evenly, add the remaining 0.5 part of AHK - Cu, stir evenly, then add 5 parts of HEPES, stir evenly to obtain Solution 2; then add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1 and 0.1 part of myristoyl hexapeptide - 16 into Solution 2, stir evenly, and then add 25 parts of betaine propanediol, stir evenly to obtain Comparative Composition 3 (denoted as Sample 3).

[0096] Comparative Example 4

[0097] Mix 3 parts of adenosine tartrate with 34.9 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10 wt% sodium hydroxide aqueous solution, and after stirring evenly, obtain Solution 1; Mix 1 part of AHK-Cu, 5 parts of HEPES, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly to obtain Solution 2; Add Solution 2 to Solution 1 and stir evenly, then add 0.1 part of acetyl octapeptide-2, 5 parts

[0098] of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1 and 0.1 part of myristoyl hexapeptide-16, and after stirring evenly, obtain Comparative Composition 4 (denoted as Sample 4).

[0099] Comparative Example 5

[0100] Mix 10 parts of HEPES, 3 parts of adenosine tartrate with 75.6 parts of deionized water, heat up to 60 °C, after stirring evenly, cool down to room temperature to obtain Solution 1; Add 1 part of AHK-Cu, 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, 0.1 part of myristoyl hexapeptide-16 to Solution 1, and after stirring evenly, obtain Solution 2; Add 5 parts of 1,2-pentanediol to Solution 2, and after stirring evenly, obtain Comparative Composition 5 (denoted as Sample 5).

[0101] Comparative Example 6

[0102] Mix 10 parts of HEPES, 3 parts of adenosine tartrate with 50.6 parts of deionized water, heat up to 60 °C, after stirring evenly, cool down to room temperature to obtain Solution 1; Add 1 part of AHK-Cu, 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, 0.1 part of myristoyl hexapeptide-16 to Solution 1, and after stirring evenly, obtain Solution 2; Add 8.5 parts of betaine, 16.5 parts of propanediol, 5 parts of 1,2-pentanediol to Solution 2, and after stirring evenly, obtain Comparative Composition 6. (Denoted as Sample 6)

[0103] Comparative Example 7

[0104] Mix 3 parts of adenosine tartrate with 60.6 parts of deionized water, heat up to 60 °C, after stirring evenly, cool down to room temperature to obtain Solution 1; Add 1 part of AHK-Cu, 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, 0.1 part of myristoyl hexapeptide-16 to Solution 1 respectively, and after stirring evenly, obtain Solution 2; Add 25 parts of betaine propanediol, 5 parts of 1,2-pentanediol to Solution 2, and after stirring evenly, obtain Comparative Composition 7. (Denoted as Sample 7)

[0105] Example 1

[0106] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 34.9 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK - Cu, 25 parts of betaine propanediol and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain anti - hair loss composition 1 after stirring evenly.

[0107] Example 2

[0108] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 35.6 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature to obtain Solution 1; mix 1 part of AHK - Cu, 25 parts of betaine propanediol and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain anti - hair loss composition 2 after stirring evenly.

[0109] Example 3

[0110] Mix 5 parts of HEPES, 2 parts of adenosine tartrate with 35.9 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK - Cu, 25 parts of betaine propanediol and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain anti - hair loss composition 3 after stirring evenly.

[0111] Example 4

[0112] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK - Cu, 25 parts of betaine propylene glycol and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, and 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain the anti - hair - loss composition 4 after stirring evenly.

[0113] Example 5

[0114] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK - Cu, 25 parts of betaine malate, and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, and 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain the anti - hair - loss composition 5 after stirring evenly.

[0115] Example 6

[0116] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK - Cu, 25 parts of betaine citrate, and 10 parts of deionized water, add the mixture to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide - 1, and 0.1 part of myristoyl hexapeptide - 16 to Solution 2, and obtain the anti - hair - loss composition 6 after stirring evenly.

[0117] Example 7

[0118] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK-Cu, 25 parts of betaine lactate and 10 parts of deionized water, add it to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 to Solution 2, and obtain anti-hair loss composition 7 after stirring evenly.

[0119] Example 8

[0120] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK-Cu, 25 parts of L-carnitine malate and 10 parts of deionized water, add it to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 to Solution 2, and obtain anti-hair loss composition 8 after stirring evenly.

[0121] Example 9

[0122] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and obtain Solution 1 after stirring evenly; mix 1 part of AHK-Cu, 25 parts of L-carnitine citrate and 10 parts of deionized water, add it to Solution 1 after stirring evenly, and obtain Solution 2 after stirring evenly; add 0.1 part of acetyl octapeptide-2, 5 parts of decarboxy carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 to Solution 2, and obtain anti-hair loss composition 9 after stirring evenly.

[0123] Example 10

[0124] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 20 parts of betaine propanediol, 5 parts of betaine citrate, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2, 5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 into Solution 2 respectively, stir evenly to obtain anti-hair loss composition 10.

[0125] Example 11

[0126] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 38.9 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2, 2.5 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 into Solution 2, stir evenly to obtain anti-hair loss composition 11.

[0127] Example 12

[0128] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 39.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2, 2 parts of decarboxylated carnosine hydrochloride, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 into Solution 2, stir evenly to obtain anti-hair loss composition 12.

[0129] Example 13

[0130] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 41.4 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, stir evenly, then cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2, 0.2 part of biotin tripeptide-1, and 0.1 part of myristoyl hexapeptide-16 to Solution 2, stir evenly to obtain anti-hair loss composition 13.

[0131] Example 14

[0132] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 41.5 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, stir evenly, then cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2 and 0.2 part of biotin tripeptide-1 to Solution 2, stir evenly to obtain anti-hair loss composition 14

[0133] Example 15

[0134] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 41.6 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, stir evenly, then cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2 and 0.1 part of myristoyl hexapeptide-16 to Solution 2, stir evenly to obtain anti-hair loss composition 15.

[0135] Example 16

[0136] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 41.7 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, stir evenly, then cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide-2 to Solution 2, stir evenly to obtain anti-hair loss composition 16.

[0137] Example 17

[0138] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.6 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK - Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide - 2, 5 parts of decarboxy carnosine hydrochloride, and 0.1 part of myristoyl hexapeptide - 16 to Solution 2, stir evenly to obtain anti - hair - loss composition 17.

[0139] Example 18

[0140] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 35.7 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK - Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.1 part of acetyl octapeptide - 2, 0.2 part of biotin tripeptide - 1, and 5 parts of decarboxy carnosine hydrochloride to Solution 2, stir evenly to obtain anti - hair - loss composition 18.

[0141] Example 19

[0142] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.5 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK - Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride, 0.1 part of myristoyl hexapeptide - 16, and 0.2 part of biotin tripeptide - 1 to Solution 2, stir evenly to obtain anti - hair - loss composition 19.

[0143] Example 20

[0144] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36 parts of deionized water and 15 parts of 1,2 - pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution to obtain Solution 1; mix 1 part of AHK - Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and then add it to Solution 1, stir evenly to obtain Solution 2; add 0.2 part of biotin tripeptide - 1 and 5 parts of decarboxy carnosine hydrochloride to Solution 2, stir evenly to obtain anti - hair - loss composition 20.

[0145] Example 21

[0146] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.7 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, after stirring evenly, obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride and 0.1 part of myristoyl hexapeptide-16 to Solution 2, stir evenly to obtain the anti-hair loss composition 21.

[0147] Example 22

[0148] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 41.5 parts of deionized water and 15 parts of 1,2-pentanediol, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, after stirring evenly, obtain Solution 2; add 0.2 part of biotin tripeptide-1 and 0.1 part of myristoyl hexapeptide-16 to Solution 2, stir evenly to obtain the anti-hair loss composition 22.

[0149] Example 23

[0150] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 35.697 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 part of xanthan gum and 0.2 part of EDTA2Na, heat up to 60 °C, after stirring evenly, cool down to room temperature, add 0.7 part of 10% sodium hydroxide aqueous solution, and stir evenly to obtain Solution 1; mix 0.4 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, after stirring evenly, obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride to Solution 2, stir evenly to obtain the anti-hair loss composition 23.

[0151] Example 24

[0152] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 35.5 parts of deionized water and 15 parts of 1,2-pentanediol. Heat the mixture to 60 °C, stir evenly, then cool down to room temperature. Add 0.7 part of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1; mix 0.4 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, then stir evenly to obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride, 0.1 part of myristoyl hexapeptide-16, 0.1 part of acetyl octapeptide-20 and 0.2 part of biotin tripeptide-1 to Solution 2, stir evenly to obtain the anti-hair loss composition 24.

[0153] Example 25

[0154] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 37.197 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 part of xanthan gum and 0.2 part of EDTA 2Na. Heat the mixture to 60 °C, stir evenly, then cool down to room temperature. Add 0.7 part of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1; mix 0.4 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, then stir evenly to obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride to Solution 2, stir evenly to obtain the anti-hair loss composition 27.

[0155] Example 26

[0156] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 37 parts of deionized water and 15 parts of 1,2-pentanediol. Heat the mixture to 60 °C, stir evenly, then cool down to room temperature. Add 0.7 part of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1; mix 0.4 part of AHK-Cu, 25 parts of betaine propanediol and 10 parts of deionized water, stir evenly and add it to Solution 1, then stir evenly to obtain Solution 2; add 5 parts of decarboxy carnosine hydrochloride, 0.1 part of myristoyl hexapeptide-16, 0.1 part of acetyl octapeptide- and 0.2 part of biotin tripeptide-1 to Solution 2, stir evenly to obtain the anti-hair loss composition 26.

[0157] Example 27

[0158] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.597 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 parts of xanthan gum, and 0.2 parts of EDTA 2Na. Heat the mixture to 60°C, stir evenly, then cool it down to room temperature. Add 0.7 parts of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1. Mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly, and then add the mixture to Solution 1. Stir evenly to obtain Solution 2. Add 5 parts of decarboxylated carnosine hydrochloride to Solution 2 and stir evenly to obtain the anti-hair loss composition 27.

[0159] Example 28

[0160] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.197 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 parts of xanthan gum, and 0.2 parts of EDTA 2Na. Heat the mixture to 60°C, stir evenly, then cool it down to room temperature. Add 0.7 parts of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1. Mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly, and then add the mixture to Solution 1. Stir evenly to obtain Solution 2. Add 5 parts of decarboxylated carnosine hydrochloride, 0.1 part of myristoyl hexapeptide-16, 0.1 part of acetyl octapeptide-2, and 0.2 part of biotin tripeptide-1 to Solution 2 and stir evenly to obtain the anti-hair loss composition 28.

[0161] Example 29

[0162] Mix 5 parts of HEPES, 1.5 parts of adenosine tartrate with 36.8 parts of deionized water and 15 parts of 1,2-pentanediol. Heat the mixture to 60°C, stir evenly, then cool it down to room temperature. Add 0.7 parts of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1. Mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly, and then add the mixture to Solution 1. Stir evenly to obtain Solution 2. Add 5 parts of decarboxylated carnosine hydrochloride to Solution 2 and stir evenly to obtain the anti-hair loss composition 29.

[0163] Example 30

[0164] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 35.097 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 parts of xanthan gum, and 0.2 parts of EDTA2Na. Heat the mixture to 60°C, stir evenly, then cool it down to room temperature. Add 0.7 parts of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1. Mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly, and then add the mixture to Solution 1. Stir evenly to obtain Solution 2. Add 5 parts of decarboxylated carnosine hydrochloride to Solution 2 and stir evenly to obtain the anti-hair loss composition 30.

[0165] Example 31

[0166] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 34.697 parts of deionized water, 15 parts of 1,2-pentanediol, 0.003 part of xanthan gum, and 0.2 part of EDTA 2Na. Heat to 60 °C, stir evenly, then cool to room temperature. Add 0.7 part of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, then stir evenly to obtain Solution 2; add 5 parts of decarboxylated carnosine hydrochloride, 0.1 part of myristoyl hexapeptide-16, 0.1 part of acetyl octapeptide-2, and 0.2 part of biotin tripeptide-1 to Solution 2, stir evenly to obtain the anti-hair loss composition 31.

[0167] Example 32

[0168] Mix 5 parts of HEPES, 3 parts of adenosine tartrate with 34.697 parts of deionized water, 15 parts of 1,2-pentanediol. Heat to 60 °C, stir evenly, then cool to room temperature. Add 0.7 part of 10% sodium hydroxide aqueous solution and stir evenly to obtain Solution 1; mix 1 part of AHK-Cu, 25 parts of betaine propanediol, and 10 parts of deionized water, stir evenly and add it to Solution 1, then stir evenly to obtain Solution 2; add 5 parts of decarboxylated carnosine hydrochloride to Solution 2, stir evenly to obtain the anti-hair loss composition 32.

[0169] Analysis of comparative examples:

[0170] 1. Analyze the processes of Sample 1, Sample 2, Sample 3, Sample 4, and the anti-hair loss composition 1, and the results are as follows:

[0171] Sample 1: After adding AHK-Cu to Solution 1, Solution 1 becomes a blue turbid liquid. After standing for 6 hours, the turbid liquid becomes clear. After adding the remaining components, it becomes a blue clear and transparent liquid. There is precipitation after observing the stability for a period of time (1 month). The results are as Figure 1 shown.

[0172] Sample 2: Based on the precipitation of Sample 1 after standing for a period of time, improve Comparative Example 1 by adding another 10 parts of 1,2-pentanediol. There is no precipitation after standing for a period of time. However, there is still turbidity after adding AHK-Cu.

[0173] Sample 3: Based on the phenomenon that the sample 2 was still turbid after adding AHK-Cu, the process of Comparative Example 2 was improved. Adenosine tartrate was mixed with deionized water and 1,2-pentanediol to obtain Solution 1; 0.5 part of AHK-Cu was added to Solution 1, and after stirring evenly, a green transparent solution was obtained. Then the remaining 0.5 part of AHK-Cu was added, stirred evenly, and then HEPES was added, and the color became darker (possibly because HEPES, as a buffer, has the effect of adjusting pH). However, precipitation occurred after adding carnosine hydrochloride (as shown in Figure 2 ), and the phenomenon remained unchanged after adding betaine propanediol and other components.

[0174] Sample 4: Based on the situation that precipitation occurred after adding carnosine hydrochloride in Sample 3 and adding betaine propanediol did not improve it, the process of Comparative Example 3 was improved: 10 wt% sodium hydroxide aqueous solution was used to adjust the pH to increase the solubility of adenosine tartrate and carnosine hydrochloride; then AHK-Cu, HEPES and betaine propanediol were pre-dissolved to maintain the stability of AHK-Cu, and then they were added to Solution 1 respectively and stirred evenly with other components. This process solved both the problem that the solution became turbid after adding AHK-Cu in Comparative Example 1 and Comparative Example 2 and the problem that precipitation occurred after adding carnosine hydrochloride in Comparative Example 3. The sample 4 of Comparative Example 4 was observed for stability at 4°C, -15°C, room temperature (25°C) and 45°C, and the results are as shown in 3. The sample 4 under the condition of 45°C turned greenish-black.

[0175] In view of the problem that the sample 4 changed color quickly under the condition of 45°C and the problem that it was difficult to pre-dissolve HEPES, AHK-Cu and betaine propanediol, the process of Example 1 thus appeared.

[0176] 2. Since HEPES has the effect of loosening the cutin layer and betaine propanediol has the effect of improving the utilization rate of active substances, Samples 5, 6, 7 and the anti-hair loss composition 1 were designed for transdermal absorption tests to judge the influence of betaine propanediol and HEPES on the penetration of active substances:

[0177] Using pig skin as a model, in vitro skin penetration tests were carried out through Franz diffusion cells to detect the cumulative transdermal amount of polypeptides at each time point, so as to evaluate the skin penetration performance of the anti-hair loss composition 1.

[0178] From Figure 4 、 5 、6, it can be seen that the cumulative polypeptide penetration amount at 24 h:

[0179] Combined with the cumulative penetration trend graph, the release times of biotin tripeptide-1, AHK-Cu, and decarboxy carnosine hydrochloride in the anti-hair loss composition 1 are all earlier than those of the samples in the comparative examples, and the overall release trend is relatively stable and continuous. Moreover, it can be seen from the penetration enhancement graph of AHK-Cu that HEPES helps to maintain the stability of AHK-Cu and the stability of the entire system, facilitating transdermal penetration. Thus, it can be seen that after the anti-hair loss composition 1 contains the supramolecular penetration enhancer (i.e., betaine propylene glycol) and HEPES, it can improve the transdermal absorption of the active substances, maximize the bioavailability of the active substances, and maintain the stability of the system, more effectively exerting its effects of promoting the generation of VEGF in the skin, strengthening follicle activity, increasing the antioxidant capacity of the scalp, promoting hair growth, and strengthening hair fixation.

[0180] Test Example 1

[0181] Perform antibacterial, oil control, and anti-hair loss tests on adenosine tartrate.

[0182] 1. Antibacterial test:

[0183] Malassezia is a kind of fungus that takes sebum as its "food" and originally lives on the oily skin obediently, coexisting peacefully with us. When the sebaceous glands secrete vigorously, Malassezia will overgrow, and its metabolite lipase can turn triglycerides in the hair follicles into free fatty acids, stimulating the hair follicle orifice to produce a large amount of desquamation, resulting in the blockage and rupture of the hair follicle duct, leading to an inflammatory reaction. Inhibiting Malassezia will reduce the stimulation of multiple hair follicles.

[0184] Add 3.0 - 5.0 mL of normal saline to the slant of the cultured bacterial strain, and scrape the bacterial plaque with a sterile cotton swab. Use a pipette gun to aspirate and transfer it to a sterile test tube, dilute the bacterial solution with normal saline, mix it by oscillation, then measure the absorbance value and select the bacterial suspension with an appropriate absorbance value. Place the prepared bacterial solution in a 4°C refrigerator for later use. Take 0.1 mL of the diluted bacterial solution and add it to a test tube containing 5 mL of the sample, mix it quickly, and immediately start timing for 5 minutes; after acting for the specified time, take 0.5 mL of the mixed solution of the test bacteria and the sample, add it to a test tube containing 4.5 mL of sterilized PBS, and mix it well; after standing for 10 minutes, aspirate 1 mL of the sample solution (or after appropriate dilution, take the diluted solutions of 2 - 3 dilution factors) into a sterilized petri dish, pour 15 mL of Malassezia agar medium cooled to 40°C - 45°C, rotate the petri dish to make it fully uniform, and make 2 parallels for each dilution factor. After the agar solidifies, turn the petri dish over and culture it in a 37°C incubator for 48 hours, then perform viable bacterial colony counting; use PBS instead of the test sample and operate according to the above steps simultaneously as the control sample; repeat the experiment 3 times, calculate the average value, and calculate the antibacterial rate.

[0185] The results are shown in Table 1:

[0186] Table 1. Inhibition of bacterial strains by adenosine tartrate solution

[0187]

[0188] As can be seen from Table 1:

[0189] After 5 minutes of action at a test concentration of 1.95%, the antibacterial rate of adenosine tartrate against Malassezia furfur was 78.33%, indicating that adenosine tartrate has antibacterial activity against Malassezia furfur.

[0190] 3. Oil control test:

[0191] Thirty volunteers used 2% adenosine tartrate shampoo for 14 days and 28 days, and then used a Sebumcter (skin oil tester) to measure the skin oil content in the test area.

[0192] From Figure 7 and Table 2, it can be seen that:

[0193] Table 2. Human effect diagram of using 2% adenosine tartrate shampoo

[0194]

[0195] After 14 days and 28 days of using 2% adenosine tartrate by thirty volunteers, the skin oil content in the test area was significantly reduced compared with the initial value, indicating that the test sample (adenosine tartrate) has an oil control effect.

[0196] 3. Anti - hair loss test:

[0197] The sample was used to conduct a TGF - β2 (transforming growth factor β) gene expression experiment on human keratinocytes, with minoxidil as a control. The detection results of the relative expression levels of TGF - β2 in different groups are shown in detail in Figure 8 .

[0198] From Figure 8 it can be seen that:

[0199] Compared with the negative control group (NC), the TGF - β2 gene expression level in the positive control group (PC) decreased significantly (P < 0.05), indicating that this experiment was effective.

[0200] Compared with the negative control group (NC), at test concentrations of 0.001% and 0.0005%, the TGF - β2 gene expression levels of adenosine tartrate decreased significantly (P < 0.05), and the inhibition rates were 53% and 50% respectively, indicating that adenosine tartrate can inhibit the secretion of TGF - β2 at concentrations of 0.001% and 0.0005% to achieve the anti - hair loss effect.

[0201] Test Example 2

[0202] Stability tests were carried out on the anti - hair loss compositions 1 - 32.

[0203] Table 3. Stability of the anti - hair - loss compositions 1 - 32 in appearance after 3 months under different conditions

[0204]

[0205]

[0206]

[0207]

[0208] According to the stability results of the appearance of the anti - hair - loss compositions after 3 months under different conditions in Table 3, the anti - hair - loss composition 1 was preferably selected for the stability test under different pH (arginine, citric acid) conditions, and the results are shown in Table 4 below:

[0209] Table 4. Stability of the anti - hair - loss composition 1 in appearance under different pH conditions

[0210]

[0211] Test Example 3

[0212] Safety, anti - inflammatory, and anti - hair - loss tests were carried out on the anti - hair - loss composition 1 based on the stability situation.

[0213] 1. Safety test:

[0214] Explore the effects of the anti - hair - loss composition 1 on the survival rates of keratinocytes, human fibroblasts, and dermal papilla cells. The specific method is as follows:

[0215] (1) Based on the keratinocyte cytotoxicity detection test, the results are as Figure 9 shown. It can be seen from Figure 9 that for the anti - hair - loss composition 1 based on keratinocytes, the IC 50 = 2.2715% (95% CI: 1.8309% - 2.7120%).

[0216] (2) Based on the human fibroblast cytotoxicity detection test, the results are as Figure 10 shown. It can be seen from Figure 10 that for the anti - hair - loss composition 1 based on human fibroblasts, the IC 50 = 1.2997% (95% CI: 0.8546% - 1.7449%).

[0217] (3) Based on the dermal papilla cell cytotoxicity detection test, the results are as Figure 11 shown. It can be seen from Figure 11 that the anti - hair - loss composition 1 did not show obvious cytotoxicity within the concentration range of 0.3125%.

[0218] 2. Anti-inflammatory and anti-hair loss efficacy test:

[0219] In this test, hair papilla cells were used as the test objects, and DHT (human dihydrotestosterone) was used to stimulate the hair papilla cells. A BC group (cells + culture medium), an NC group (DHT + cells + culture medium), a PC group (DHT + cells + culture medium + minoxidil), and a sample group (DHT + cells + culture medium + anti-hair loss composition 1) were set up. The anti-hair loss efficacy of the anti-hair loss composition 1 was evaluated by detecting the changes in IL-6, IL-1β, and VEGF levels after the anti-hair loss composition 1 acted on the hair papilla cells.

[0220] (1) IL-6 test:

[0221] The results are as follows Figure 12 As shown, from Figure 12 It can be seen that:

[0222] Compared with the blank control group (BC), the IL-6 secretion level of the negative control group (NC) was significantly increased, indicating that the stimulation conditions of this test were effective;

[0223] Compared with the negative control group (NC), the IL-6 secretion level of the positive control group (PC) was significantly reduced, indicating that the positive conditions of this test were effective;

[0224] Compared with the negative control group (NC), the anti-hair loss composition 1 can significantly reduce the IL-6 content, indicating that the anti-hair loss composition 1 has a certain effect in correcting the abnormal expression of signal molecules that cause androgenic alopecia, thereby achieving a certain degree of hair growth effect.

[0225] (2) IL-1β test:

[0226] The results are as follows Figure 13 As shown, from Figure 13 It can be seen that:

[0227] Compared with the blank control group (BC), the IL-1β secretion level in the negative control group (NC) was significantly increased, indicating that the stimulation conditions of this test were effective.

[0228] Compared with the negative control group (NC), the IL-1β secretion level of the positive control group (PC) was significantly reduced, indicating that the positive conditions of this test were effective.

[0229] Compared with the negative control group (NC), the anti-hair loss composition 1 can significantly reduce the IL-1β content, indicating that the anti-hair loss composition 1 can reduce the occurrence of inflammatory response, help restore the healthy balance of the scalp, and reshape the scalp's own immunity, thereby achieving a certain degree of hair growth effect.

[0230] (3) VEGF test:

[0231] VEGF is a known important vascular endothelial growth factor, a highly specific vascular endothelial cell growth-promoting factor, which has the effects of promoting increased vascular permeability, extracellular matrix degeneration, vascular endothelial cell migration, proliferation, and angiogenesis. Therefore, the reduction of VEGF will directly lead to insufficient vascularization, which is an important factor in hair follicle atrophy.

[0232] The results are as Figure 14 shown, and it can be known from Figure 14 that:

[0233] Compared with the blank control group (BC), the VEGF secretion level of the negative control group (NC) was significantly decreased, indicating that the stimulation conditions of this test were effective;

[0234] Compared with the negative control group (NC), the VEGF secretion level of the positive control group (PC) was significantly increased, indicating that the positive conditions of this test were effective;

[0235] Compared with the negative control group (NC), the anti-hair loss composition 1 could significantly increase the VEGF content, indicating that the anti-hair loss composition 1 could improve the local blood circulation around the hair follicles, promote the supply of oxygen and nutrients required for hair growth, nourish the hair follicles, and thus provide support for hair growth at the vascular system level.

[0236] As can be seen from the above, based on dermal papilla cells, compared with the control group, at a concentration of 0.3125%, the VEGF content of the sample anti-hair loss composition 1 increased significantly, with an increase rate of 18.68%, and the contents of IL-1β and IL-6 decreased significantly, with inhibition rates of 72.32% and 39.39% respectively. It is considered that the anti-hair loss composition 1 achieves anti-inflammatory and anti-hair loss effects at this concentration by increasing the VEGF content and inhibiting the contents of IL-1β and IL-6.

[0237] In summary, the present invention provides an anti-hair loss composition, its preparation method and application. The anti-hair loss composition includes: a first composition, a second composition, a third composition, and excipients. The first composition includes: HEPES, adenosine tartrate, 1,2-pentanediol. The second composition includes: AHK-Cu, supramolecular solvent. The third composition includes: one or more of acetyl octapeptide-2, decarboxy carnosine hydrochloride, biotin tripeptide-1, myristoyl hexapeptide-16. The excipient is an aqueous sodium hydroxide solution. The anti-hair loss composition of the present invention can target hair loss and intercept it from multiple dimensions. First, it inhibits bacteria and reduces oiliness; secondly, it activates the self-repair of hair follicle cells by antioxidant and regulating the production of vascular endothelial growth factor; then it promotes the growth of hair by promoting the production of collagen and keratin; finally, it uses a supramolecular penetration enhancer to improve the utilization rate of active ingredients and enhance the efficacy of the composition. Therefore, through multi-dimensional effects, it prevents hair loss, strengthens hair follicles, and promotes hair growth.

[0238] It should be understood that the application of the present invention is not limited to the above examples. For those of ordinary skill in the art, improvements or modifications can be made according to the above description, and all such improvements and modifications shall fall within the protection scope of the appended claims of the present invention.

Claims

1. An anti - hair loss composition, characterized in that, Comprising: A first composition, a second composition, a third composition, and excipients; The first composition comprises: HEPES, adenosine tartrate, 1,2-pentanediol; The second composition comprises: AHK-Cu, supramolecular solvent; The third composition comprises one or more of acetyl octapeptide-2, decarboxy carnosine hydrochloride, biotin tripeptide-1, myristoyl hexapeptide-16; The excipient is an aqueous sodium hydroxide solution.

2. The anti - hair - loss composition according to claim 1, wherein The supramolecular solvent in the second composition is one or more of betaine propylene glycol, betaine malic acid, betaine lactic acid, betaine citric acid, L-carnitine citric acid, L-carnitine malic acid.

3. The anti - hair - loss composition according to claim 1, wherein, By weight parts, the first composition comprises: 5-20 parts of HEPES, 0.1-5 parts of adenosine tartrate, 3-20 parts of 1,2-pentanediol; The second composition comprises: 0.4-15 parts of AHK-Cu, 10-40 parts of supramolecular solvent; The third composition comprises one or more of 0.1-10 parts of acetyl octapeptide-2, 0.5-6 parts of decarboxy carnosine hydrochloride, 0.1-10 parts of biotin tripeptide-1, 0.1-10 parts of myristoyl hexapeptide-16; The excipient is 0-1.0 part of an 8-12 wt% aqueous sodium hydroxide solution.

4. The anti - hair - loss composition according to claim 3, wherein, By weight parts, the third composition comprises: 0.1-10 parts of acetyl octapeptide-2, 0.5-6 parts of decarboxy carnosine hydrochloride, 0.1-10 parts of biotin tripeptide-1, 0.1-10 parts of myristoyl hexapeptide-16.

5. A method for preparing the anti - hair - loss composition according to any one of claims 1 - 4, characterized in that, Including the steps of: mixing the first composition, the second composition, the third composition, and the excipients, and stirring evenly to obtain the anti-hair loss composition.

6. The preparation method of the anti - hair - loss composition according to claim 5, wherein, Specifically including the steps: Mixing the first composition, deionized water, and the excipient to obtain Solution 1; Mixing the second composition and deionized water, and then adding them to Solution 1 to obtain Solution 2; Adding the third composition to Solution 2 to obtain the anti-hair loss composition.

7. The preparation method of the anti - hair - loss composition according to claim 6, characterized in that, Specifically including the steps: Mixing the first composition with deionized water, heating, stirring evenly, then cooling to room temperature, adding the excipient, and stirring evenly to obtain Solution 1; Mixing the second composition with deionized water, stirring evenly, adding them to Solution 1, and stirring evenly to obtain Solution 2; Adding the third composition to Solution 2 and stirring evenly to obtain the anti-hair loss composition.

8. The preparation method of the anti - hair - loss composition according to claim 7, wherein, The rotation speed of stirring is 200-600 revolutions per minute; The temperature of heating is 50-80 °C.

9. Use of the anti-hair loss composition according to any one of claims 1-4 in the preparation of anti-hair loss products.

10. Use of the anti - hair - loss composition according to claim 9 in the preparation of an anti - hair - loss product, characterized in that, The anti-hair loss products are one or more of anti-hair loss shampoo, anti-hair loss hair conditioner, anti-hair loss essence.

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