A male oil control composition containing a norway spruce leaf extract and application thereof

Abstract

The application discloses a male oil control composition containing Norway spruce leaf extract and application, and belongs to the technical field of cosmetics. The oil control composition provided by the application comprises Norway spruce leaf extract, soapberry fruit extract, Schisandra chinensis extract, Albizia kalkora seed extract, Elaeagnus angustifolia bark extract and Phellodendron bark extract. The application selects suitable types of components to cooperate with each other, and the components are synergistic with each other, so that the oil secretion can be controlled, the scalp microorganisms can be inhibited, the 5alpha-reductase can be inhibited, and the scalp temperature can be appropriately reduced, thereby excellent improvement of male scalp oiliness, reduction of dandruff and hair loss can be realized. Moreover, the composition can effectively maintain the moisture content of the scalp keratin layer, and the dryness and itching phenomenon can be avoided.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to a male oil control composition containing Norway spruce leaf extract and application. BACKGROUND

[0002] Shampoo is an essential washing and caring product in people's life. At present, there are many types of shampoo on the market, but the shampoo developed for men is relatively rare. Compared with women, men have faster metabolism and are more likely to secrete oil, so oil control is very important in male shampoo.

[0003] The existing shampoo generally adds chemical cleaning ingredients, but if the content of the chemical cleaning ingredients in the washing and caring product is too high, it will lead to excessive cleaning of oil, which may promote the secretion of oil in the long term, and reduce the moisture content of the scalp stratum corneum, thereby causing dryness and itching of the scalp. SUMMARY

[0004] The purpose of the present application is to overcome the shortcomings of the prior art and provide a male oil control composition and application which can effectively and long-acting improve the male scalp oil secretion, reduce dandruff and hair loss from the aspects of controlling oil secretion, inhibiting scalp microorganisms and inhibiting 5alpha-reductase, and can maintain the moisture content of the scalp stratum corneum at the same time, avoiding dryness and itching.

[0005] To achieve the above purpose, in the first aspect of the present application, the present application provides a male oil control composition containing Norway spruce leaf extract, which comprises Norway spruce leaf extract, soapberry fruit extract, Schisandra extract, greyhairsesed extract, paliurus hexander bark extract and phellodendron bark extract.

[0006] The present application can control oil secretion, inhibit scalp microorganisms, inhibit 5alpha-reductase and appropriately reduce the temperature of the scalp by selecting suitable types of components and mutual cooperation between the components, thereby achieving excellent improvement of male scalp oil secretion, reduction of dandruff and hair loss; and the composition can also effectively maintain the moisture content of the scalp stratum corneum, avoiding dryness and itching.

[0007] Specifically, the inventors speculate that the Norway spruce leaf extract contains abundant polysaccharides, flavonoids, polyphenols and other components, which can inhibit the secretion of lipids by sebaceous gland cells, triterpenoid components can improve the water and oil balance of the skin, reduce dryness and itching of the skin; in addition, inhibiting the secretion of oil may reduce the production of inflammation to some extent, and the extract also has anti-inflammatory components such as flavonoids, so it also has a certain skin anti-inflammatory effect. The saponin components in the soapberry fruit extract are a natural surfactant, which has excellent cleaning ability and natural anti-inflammatory and antibacterial properties, and in addition, studies have tested that soapberry saponin can inhibit excessive accumulation of sebum through in vitro experiments, that is, the soapberry fruit extract has a certain oil control effect. The lignan components in the Schisandra extract can promote the proliferation of dermal papilla cells, thereby regulating the cycle of hair follicles and inducing hair follicle regeneration, which can reduce the alopecia caused by damaged hair follicles; and schizandrin B has strong anti-inflammatory efficacy. The grey hair seed extract can activate the expression of stress regulatory factors to control skin stress. The jujube bark extract contains a large amount of natural saponins, which can effectively inhibit the release of histamine, reduce inflammation and itching, balance the bacterial flora of the scalp, and reduce the severity of dandruff; in addition, it can also regulate sebum secretion. The berberine in the cortex phellodendri extract has a strong local astringent cooling effect, which can be used to reduce the heat generated by the scalp. In addition to the above speculations, the components should also have corresponding interactions among them, so as to jointly act in the directions of inhibiting androgen metabolism, reducing scalp temperature, moderate cleaning, inhibiting scalp microorganisms, etc., thereby effectively reducing the oil of the male scalp, improving the problem of hair loss and dandruff, and achieving the effect of maintaining the scalp stratum corneum barrier, avoiding the phenomenon of itching caused by the decrease of the water content of the scalp stratum corneum.

[0008] As a preferred embodiment of the oil control composition of the present application, the oil control composition comprises the following components by mass fraction: Norway spruce leaf extract 0.5-5 parts, soapberry fruit extract 2-8 parts, Schisandra extract 0.2-0.8 parts, grey hair seed extract 0.05-0.5 parts, jujube bark extract 0.05-0.4 parts, cortex phellodendri extract 0.6-5 parts.

[0009] As a preferred embodiment of the oil control composition of the present application, the oil control composition comprises the following components by mass fraction: Norway spruce leaf extract 1-3 parts, soapberry fruit extract 4-6 parts, Schisandra extract 0.4-0.6 parts, grey hair seed extract 0.1-0.3 parts, jujube bark extract 0.1-0.3 parts, cortex phellodendri extract 1-3 parts.

[0010] The application researches and finds that when the mass fraction of the components is further controlled within the above range, the product obtained can achieve good repair of the scalp barrier on the basis of oil control, dandruff reduction and hair loss, and can further achieve long-term oil control, dandruff reduction and hair loss effects; that is, the comprehensive effect is more excellent.

[0011] As a preferred embodiment of the oil control composition of the application, the mass percentage of the sum of the mass of the Picea abies leaf extract and the extract of Albizia lebbeck seeds to the total mass of the oil control composition is 14-30%.

[0012] Illustratively, the mass percentage of the sum of the mass of the Picea abies leaf extract and the extract of Albizia lebbeck seeds to the total mass of the oil control composition can be any point value or any two-point range value between 14-30%, such as 14%, 16%, 18%, 20%, 22%, 24%, 26%, 28%, 30%, etc.

[0013] The application researches and finds that when the mass percentage of the sum of the mass of the Picea abies leaf extract and the extract of Albizia lebbeck seeds is further controlled within the above range, the comprehensive effect of the product obtained is more excellent.

[0014] As a preferred embodiment of the oil control composition of the application, the preparation method of the Sapindus fruit extract comprises the following steps:

[0015] (1) After the Sapindus fruit is crushed, deionized water is added, and cellulase and pectinase are added for first enzymolysis. After the first enzymolysis is completed, the enzymes are inactivated, then centrifuged, and the first enzymolysis liquid is collected;

[0016] (2) Starch amylase is added to the first enzymolysis liquid for second enzymolysis. After the second enzymolysis, the enzymes are inactivated, then centrifuged, and the second enzymolysis liquid is collected and concentrated and dried to obtain the Sapindus fruit extract.

[0017] The application researches and finds that by using the above-mentioned step-by-step enzymolysis method for the Sapindus fruit extract, the active ingredients and other components can be better extracted to synergize with each other, thereby better improving the comprehensive effect of the oil control composition.

[0018] As a preferred embodiment of the oil control composition of the application, the mass ratio of the cellulase and the pectinase is (2-4):1. For example, it can be any point value or any two-point range value between 2:1, 3:1 and 4:1.

[0019] As a preferred embodiment of the oil control composition of the application, in the first enzymolysis, the mass ratio of the sum of the mass of the cellulase and the pectinase to the mass of the Sapindus fruit is 1:(8-12). For example, it can be any point value or any two-point range value between 1:8, 1:9, 1:10, 1:11 and 1:12.

[0020] As a preferred embodiment of the oil control composition of the present application, the temperature of the first enzymolysis is 45-55℃, and the time of the first enzymolysis is 1-3h.

[0021] As a preferred embodiment of the oil control composition of the present application, in the second enzymolysis, the mass ratio of amylase to soapberry fruit is 1:(18-22). For example, it can be 1:18, 1:19, 1:20, 1:21, 1:22, etc.

[0022] As a preferred embodiment of the oil control composition of the present application, the temperature of the second enzymolysis is 55-65℃, and the time of the second enzymolysis is 0.5-1.5h.

[0023] The present application research found that when further selecting the parameters of enzymolysis in the above range, the comprehensive effect of the product obtained is better.

[0024] As a preferred embodiment of the oil control composition of the present application, the mass percentage of berberine in the cortex phellodendri extract is ≥2%.

[0025] For example, the mass percentage of berberine in the cortex phellodendri extract can be ≥2% of any point value or any two-point range value, such as 2.6-3.4%, 2.6-4.2%, 3.4-4.2%, etc., or 2.6%, 2.8%, 3.0%, 3.2%, 3.4%, 3.6%, 3.8%, 4.0%, 4.2%, 4.4%, etc.

[0026] As a preferred embodiment of the oil control composition of the present application, the preparation method of the cortex phellodendri extract comprises the following steps:

[0027] (1) Add an emulsifier to liquid paraffin, then add hydrochloric acid and deionized water and mix and stir to obtain a liquid membrane;

[0028] (2) Crush the cortex phellodendri and soak it in deionized water, then filter, collect the filtrate, and obtain a mother liquor;

[0029] (3) Adjust the pH value of the mother liquor to 9-10, then add a liquid membrane with a volume of 20-40% of the mother liquor and stir, then stand and separate the layers, and collect the liquid membrane layer;

[0030] (4) Heat the liquid membrane layer, stand and separate the layers, collect the aqueous phase, and concentrate and dry to obtain the cortex phellodendri extract.

[0031] The present application research found that when the cortex phellodendri extract is prepared by this method, the interaction between the cortex phellodendri extract and other components can be effectively enhanced, thereby achieving better oil control, reducing dandruff and hair loss, and achieving excellent repair of the stratum corneum barrier of the scalp, and further achieving more long-acting oil control, reducing dandruff and hair loss.

[0032] As a preferred embodiment of the oil control composition of the present application, the mass percentage of the emulsifier in the liquid film is 2-4%, the mass percentage of the liquid paraffin is 35-45%, and the mass percentage of the hydrochloric acid is 0.5-2%.

[0033] As a preferred embodiment of the oil control composition of the present application, the emulsifier comprises at least one of Span-80 and Tween 80.

[0034] As a preferred embodiment of the oil control composition of the present application, in the step (2), the temperature of the soaking is 40-60℃, and the time of the soaking is 48-72h.

[0035] As a preferred embodiment of the oil control composition of the present application, in the step (3), the stirring speed is 50-100rpm, and the stirring time is 5-15min.

[0036] As a preferred embodiment of the oil control composition of the present application, in the step (4), the heating temperature is 80-90℃, and the heating time is 20-30min.

[0037] The present application researches and finds that when the preparation parameters of the Cortex Phellodendri extract are within the above range, the comprehensive effect of the product obtained is better.

[0038] In the second aspect of the present application, the present application provides the use of the oil control composition in the preparation of a hair care product.

[0039] Exemplarily, the hair care product comprises any one of shampoo and hair conditioner.

[0040] In the third aspect of the present application, the present application provides a shampoo, which comprises the following mass percentage of components:

[0041] 2-8% of the oil control composition of the present application, 0.7-1.2% of a thickening agent, 15-20% of a surfactant, 0.7-1% of a preservative, 0.1-0.2% of a chelating agent, 0.1-0.5% of a pH regulator, and the balance is water.

[0042] As a preferred embodiment of the shampoo of the present application, the thickening agent comprises at least one of sodium chloride, potassium chloride, and hydroxypropyl methyl cellulose.

[0043] As a preferred embodiment of the shampoo of the present application, the surfactant comprises at least one of polyquaternium-10, cocamidopropyl betaine, potassium cocoyl hydrolyzed oat protein, sodium methyl cocoyl taurate, cocoyl glycoside, sodium C14-16 olefin sulfonate, polyquaternium-7, and capryloyl glycine.

[0044] As a preferred embodiment of the shampoo of the present application, the preservative comprises at least one of sodium benzoate, phenoxyethanol, and ethylhexylglycerin.

[0045] As a preferred embodiment of the shampoo of the present application, the chelating agent comprises disodium EDTA.

[0046] As a preferred embodiment of the shampoo of the present application, the pH adjusting agent comprises citric acid.

[0047] Compared with the prior art, the present application has the following advantages:

[0048] The present application can realize excellent improvement of male scalp oiliness, reduction of dandruff and hair loss by selecting suitable types of components to cooperate with each other and synergize between components to control oil secretion, inhibit scalp microorganisms, inhibit 5α-reductase, and appropriately reduce scalp temperature; and the composition can also effectively maintain the moisture content of the scalp keratin layer to avoid dryness and itching. DETAILED DESCRIPTION

[0049] For the purpose of better illustrating the present application, technical solutions and advantages, the present application will be further described below in combination with specific examples.

[0050] The reagents, methods and equipment used in the present application are all conventional reagents, methods and equipment in the art unless otherwise specified; and the raw materials used in parallel experiments are the same batch of raw materials unless otherwise specified.

[0051] Norway spruce leaf extract: Norway spruce leaf (PICEA EXCELSA) extract, purchased from Norture Company, Canada.

[0052] Schisandra extract: purchased from Draco Natural Products Company, USA.

[0053] Greyhairy bean seed extract: purchased from Givaudan Company, Switzerland.

[0054] Ziziphus joazeiro bark extract: Ziziphus joazeiro bark extract, purchased from Green Cole Company, France.

[0055] Sapindus fruit extract 1: self-made, the preparation method comprising the following steps:

[0056] (1) commercially available Sapindus fruit was dried and crushed to obtain Sapindus fruit powder;

[0057] (2) To 20 g of Sapindus fruit powder, 200 g of deionized water was added, followed by the addition of 1.5 g of cellulase (30 U / mg, purchased from Hefei Bo Mei Biological Technology Co., Ltd.) and 0.5 g of pectinase (30 U / mg, purchased from Hefei Bo Mei Biological Technology Co., Ltd.) for the first enzymolysis, the temperature of the first enzymolysis was 50℃, the time of the first enzymolysis was 2 h, after the first enzymolysis, heating to 80℃ for 10 min to inactivate the enzyme, then centrifuged at 8000 rpm for 10 min, the supernatant was collected as the first enzymolysis liquid;

[0058] (3) To the first enzymolysis liquid, 1 g of amylase (alpha-amylase, ≥5 U / mg, purchased from Shanghai Yuan Ye Biological Technology Co., Ltd.) was added for the second enzymolysis, the temperature of the second enzymolysis was 60℃, the time of the second enzymolysis was 1 h, after the second enzymolysis, heating to 80℃ for 10 min to inactivate the enzyme, then centrifuged at 8000 rpm for 10 min, the supernatant was collected and concentrated at 45℃, then vacuum dried at 50℃ for 48 h, to obtain Sapindus fruit extract 1.

[0059] Sapindus fruit extract 2: self-made, the difference between the preparation method and that of Sapindus fruit extract 1 is that no pectinase is added, and 2 g of cellulase is added.

[0060] Sapindus fruit extract 3: self-made, the difference between the preparation method and that of Sapindus fruit extract 1 is that no amylase enzymolysis is performed, and the first enzymolysis liquid is concentrated at 45℃, then vacuum dried at 50℃ for 48 h, to obtain Sapindus fruit extract 3.

[0061] Sapindus fruit extract 4: self-made, the preparation method is to add amylase, cellulase and pectinase for enzymolysis at the same time, specifically:

[0062] (1) The commercially available Sapindus fruit was dried and crushed to obtain Sapindus fruit powder;

[0063] (2) To 20 g of Sapindus fruit powder, 200 g of deionized water was added, followed by the addition of 1.5 g of cellulase, 0.5 g of pectinase and 1 g of amylase for enzymolysis, the temperature of the enzymolysis was 55℃, the time of the enzymolysis was 2 h, after the enzymolysis, heating to 80℃ for 10 min to inactivate the enzyme, then centrifuged at 8000 rpm for 10 min, the supernatant was collected and concentrated at 45℃, then vacuum dried at 50℃ for 48 h, to obtain Sapindus fruit extract 4.

[0064] Sapindus fruit extract 5: self-made, the preparation method is to only perform amylase enzymolysis, specifically:

[0065] (1) The commercially available Sapindus fruit was dried and crushed to obtain Sapindus fruit powder;

[0066] (2) To 20 g of Sapindus fruit powder, 200 g of deionized water was added, followed by 3 g of amylase for enzymolysis, the temperature of enzymolysis was 60°C, the time of enzymolysis was 3 h, after the end of enzymolysis, heating to 80°C for 10 min to inactivate the enzyme, then centrifugation at 8000 rpm for 10 min, collecting the supernatant and rotating concentration at 45°C, then vacuum drying at 50°C for 48 h, to obtain Sapindus fruit extract 5.

[0067] Sapindus fruit extract 6: self-made, the difference between the preparation method and that of Sapindus fruit extract 1 is that protease (neutral protease, 50 U / mg, purchased from Shanghai Yuan Ye Biological Technology Co., Ltd.) is used instead of amylase.

[0068] Cortex Phellodendri extract 1: the mass percentage of berberine is 2.6%, self-made, the preparation method comprises the following steps:

[0069] (1) emulsifier Span-80 was added to liquid paraffin, followed by mixing and stirring with hydrochloric acid and deionized water to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span-80 is 2.5%, the mass percentage of liquid paraffin is 40%, and the mass percentage of hydrochloric acid is 1%;

[0070] (2) commercially available Cortex Phellodendri was dried to constant weight, crushed, and passed through a 60-mesh sieve, 10 g of undersize was collected and added to 50 g of deionized water, and soaked at 50°C for 48 h, after soaking, filtration was performed, and the filtrate was collected to obtain a mother liquor;

[0071] (3) after adjusting the pH value of the mother liquor to 9-10 using NaOH, 30% of the volume of the mother liquor was added to the liquid membrane, and stirring was performed at a speed of 80 rpm for 10 min, followed by standing and layering, and the liquid membrane layer was collected;

[0072] (4) the liquid membrane layer was heated to 85°C and maintained for 20 min to break the membrane, followed by standing and layering, the aqueous phase was collected, and rotating concentration was performed at 45°C, followed by vacuum drying at 45°C for 48 h to obtain Cortex Phellodendri extract 1.

[0073] Cortex Phellodendri extract 2: the mass percentage of berberine is 3.4%, self-made, the preparation method comprises the following steps:

[0074] (1) emulsifier Span-80 was added to liquid paraffin, followed by mixing and stirring with hydrochloric acid and deionized water to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span-80 is 3.0%, the mass percentage of liquid paraffin is 45%, and the mass percentage of hydrochloric acid is 1%;

[0075] (2) commercially available Cortex Phellodendri was dried to constant weight, crushed, and passed through a 60-mesh sieve, 10 g of undersize was collected and added to 50 g of deionized water, and soaked at 60°C for 72 h, after soaking, filtration was performed, and the filtrate was collected to obtain a mother liquor;

[0076] (3) After adjusting the pH value of the mother liquor to 9-10 using NaOH, add the liquid membrane with a volume of 40% of the mother liquor and stir at a speed of 50 rpm for 10 min, then stand to separate the layers, and collect the liquid membrane layer;

[0077] (4) Heat the liquid membrane layer to 85°C and keep for 20 min to break the membrane, then stand to separate the layers, collect the water phase, and then rotate to concentrate at 45°C, then place under vacuum drying at 45°C for 48 h to obtain Cortex Phellodendri extract 2.

[0078] Cortex Phellodendri extract 3: the mass percentage of berberine is 4.2%, self-made, and the preparation method comprises the following steps:

[0079] (1) Add the emulsifier Span-80 into liquid paraffin, then add hydrochloric acid and deionized water and mix to stir to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span-80 is 4%, the mass percentage of liquid paraffin is 42%, and the mass percentage of hydrochloric acid is 2%;

[0080] (2) Dry the commercially available Cortex Phellodendri to a constant weight, crush, pass through a 60-mesh sieve, collect 10 g of undersize, add 50 g of deionized water, and soak at 60°C for 48 h, then filter after the soaking is completed, and collect the filtrate to obtain a mother liquor;

[0081] (3) After adjusting the pH value of the mother liquor to 9-10 using NaOH, add the liquid membrane with a volume of 35% of the mother liquor and stir at a speed of 50 rpm for 10 min, then stand to separate the layers, and collect the liquid membrane layer;

[0082] (4) Heat the liquid membrane layer to 85°C and keep for 20 min to break the membrane, then stand to separate the layers, collect the water phase, and then rotate to concentrate at 45°C, then place under vacuum drying at 45°C for 48 h to obtain Cortex Phellodendri extract 3.

[0083] Cortex Phellodendri extract 4: the mass percentage of berberine is 1.6%, self-made, and the preparation method comprises the following steps:

[0084] (1) Add the emulsifier Span-80 into liquid paraffin, then add hydrochloric acid and deionized water and mix to stir to obtain a liquid membrane; in the liquid membrane, the mass percentage of Span-80 is 2%, the mass percentage of liquid paraffin is 35%, and the mass percentage of hydrochloric acid is 0.5%;

[0085] (2) Dry the commercially available Cortex Phellodendri to a constant weight, crush, pass through a 60-mesh sieve, collect 10 g of undersize, add 50 g of deionized water, and soak at 40°C for 48 h, then filter after the soaking is completed, and collect the filtrate to obtain a mother liquor;

[0086] (3) After adjusting the pH value of the mother liquor to 9-10 using NaOH, adding 20% of the volume of the liquid membrane, and stirring at a speed of 50 rpm for 10 min, then standing to separate the layers, and collecting the liquid membrane layer;

[0087] (4) Heating the liquid membrane layer to 85°C and keeping for 20 min to break the membrane, then standing to separate the layers, collecting the water phase, and then rotating to concentrate at 45°C, and then vacuum drying at 45°C for 48 h to obtain the Cortex Phellodendri extract 4.

[0088] Cortex Phellodendri extract 5: the mass percentage of berberine is 2.8%, which is self-made, and the preparation method comprises the following steps:

[0089] (1) Grinding the commercially available Cortex Phellodendri dry to a constant weight, passing through a 60-mesh sieve, and collecting 10 g of undersize for supercritical carbon dioxide extraction, wherein the entrainer used in the supercritical carbon dioxide extraction is a methanol solution containing citric acid (the mass percentage of the entrainer is 8% based on the mass of the undersize, and the mass ratio of citric acid to methanol in the entrainer is 0.2:1), the temperature of the supercritical carbon dioxide extraction is 35°C, the pressure is 25 MPa, and the time is 2 h;

[0090] (3) After the supercritical extraction, filtering, collecting the filter residue, and soaking in 1 mol / L hydrochloric acid for 10 h, filtering after the soaking is completed, rotating to concentrate the filtrate at 45°C, and then vacuum drying at 45°C for 48 h to obtain the Cortex Phellodendri extract 5.

[0091] Examples 1-5 and Comparative Examples 1-8

[0092] The examples and comparative examples of the present application provide an oil control composition, and the components (mass parts) of the composition are shown in Table 1;

[0093] Among them, W represents the mass percentage of the sum of the mass of the Picea abies leaf extract and the Astragalus melilotoides seed extract to the total mass of the oil control composition;

[0094] Table 1

[0095]

[0096] The preparation method of the oil control composition provided in Example 1 is: mixing the components to obtain the oil control composition.

[0097] The preparation methods of the oil control compositions provided in Examples 2-5 and Comparative Examples 1-8 are consistent with those of Example 1; if there is no related component, it is not added.

[0098] Example 6

[0099] The examples of the present application provide an oil control composition, and the only difference between the oil control composition and Example 1 is that the Sapindus mukorossi fruit extract 2 is used instead of the Sapindus mukorossi fruit extract 1.

[0100] Example 7

[0101] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Sapindus fruit extract 3 is used in place of Sapindus fruit extract 1.

[0102] Example 8

[0103] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Sapindus fruit extract 4 is used in place of Sapindus fruit extract 1.

[0104] Example 9

[0105] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Sapindus fruit extract 5 is used in place of Sapindus fruit extract 1.

[0106] Example 10

[0107] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Sapindus fruit extract 6 is used in place of Sapindus fruit extract 1.

[0108] Example 11

[0109] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Cortex Phellodendri extract 2 is used in place of Cortex Phellodendri extract 1.

[0110] Example 12

[0111] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Cortex Phellodendri extract 3 is used in place of Cortex Phellodendri extract 1.

[0112] Example 13

[0113] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Cortex Phellodendri extract 4 is used in place of Cortex Phellodendri extract 1.

[0114] Example 14

[0115] The present example provides an oil control composition, the only difference between the oil control composition and Example 1 is that Cortex Phellodendri extract 5 is used in place of Cortex Phellodendri extract 1.

[0116] Comparative Example 9

[0117] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0118] Comparative example 10

[0119] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0120] Comparative example 11

[0121] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0122] Comparative example 12

[0123] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0124] Comparative example 13

[0125] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0126] Comparative example 14

[0127] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0128] Application examples 1-14, comparative application examples 1-14

[0129] The present application comparative example provides an oil control composition, the only difference between the oil control composition and example 1 is that the Norway spruce leaf extract is replaced by tremella extract (purchased from Hubei Xinyu Hong Biological Medicine Technology Co., Ltd.).

[0130] Table 2

[0131]

[0132]

[0133] The preparation method of the shampoo provided in application example 1 comprises the following steps:

[0134] (1) The polyquaternium-10 is added into the deionized water and stirred to disperse, and after uniform dispersion, other materials of the phase A are added, and after heating to 85℃, the stirring is uniform, and then the temperature is kept for standby, and the pre-prepared phase A is obtained;

[0135] (2) The components of the phase B are added at 80-85℃, and the stirring is uniform until transparent, and the temperature is kept for defoaming;

[0136] (3) The temperature is reduced to below 45℃, and the raw materials of the phase C are added one by one, and the stirring is uniform;

[0137] (4) The raw materials of the phase D (the oil control composition is dissolved with an appropriate amount of water first) are continuously added, and the mixing is uniform, and the shampoo is obtained.

[0138] The preparation methods of the shampoos provided in application examples 2-14 and comparative application examples 1-14 are consistent with those of application example 1, and no related components are added.

[0139] Application example 15

[0140] The shampoo provided in the application example is different from that of application example 1 in that the mass percentage of the oil control composition is 7%.

[0141] Application example 16

[0142] The shampoo provided in the application example is different from that of application example 1 in that the mass percentage of the oil control composition is 2%.

[0143] Blank application example

[0144] The shampoo provided in the blank application example is different from that of application example 1 in that no oil control composition is added.

[0145] Effect example 1

[0146] The effect of the oil control composition prepared in examples 1-14 and comparative examples 1-14 is explored in the effect example, which includes the following aspects:

[0147] 1. Anti-inflammatory ability

[0148] The test of the anti-inflammatory ability comprises the following steps:

[0149] S1, plating: the RAW264.7 cells in the logarithmic growth phase are inoculated in a 24-well plate, and the cell density is 2×10 50.5 mL per well, 0.5 mL of sample was added after the cells were plated, and the plate was labeled on the upper cover of the cell plate, and then placed in a carbon dioxide incubator for incubation for 24 h;

[0150] S2, sample treatment: after the cells were incubated for 24 h, the supernatant was removed, and 0.5 mL of the following sample was added: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone (cell culture medium containing 0.001 wt% dexamethasone) + LPS (1 μg / mL); ④ experimental group: composition (cell culture medium containing 0.5 wt% of the oil control composition of the examples or comparative examples) + LPS (1 μg / mL);

[0151] S3, detection of inflammatory factors in cell supernatant: after the sample treatment cells were incubated for 24 h, the supernatant was sucked into a centrifuge tube with a pipette, centrifuged at 1000 r / min for 5 min, the precipitate was discarded, and the supernatant was taken, and the change in the amount of inflammatory factors in the supernatant was detected with a Xibosheng TNF-α inflammatory factor detection kit;

[0152] The inhibition rate of TNF-α was calculated, and the TNF-α inhibition rate % = (model group TNF-α amount - experimental group or positive control group TNF-α amount) / model group TNF-α amount x 100%; wherein, the higher the TNF-α inhibition rate %, the stronger the anti-inflammatory ability;

[0153] The results obtained are shown in Table 3.

[0154] 2, inhibition ability of 5α-reductase

[0155] The test of the inhibition ability of 5α-reductase includes the following steps:

[0156] S1, sample tube: 1 mL of sample liquid (2 wt% of the oil control composition of the examples or comparative examples), 1 mL of enzyme liquid, 1 mL of NADPH solution and 1 mL of testosterone solution were added to the test tube, and shaken gently, 200 μL was taken from the 96-well enzyme-labeled plate with a pipette, three parallels were made for each sample, and placed in an enzyme-labeled instrument for detection, and the absorbance value was measured at 340 nm as the first measurement value A 样品0 ; after being placed in a 37°C incubator for 20 minutes, the absorbance value was measured at 340 nm as the second measurement value A 样品20 ; the positive control used 2.5 mmol / L finasteride to replace the sample liquid;

[0157] S2, enzyme tube: 1 mL of PBS solution, 1 mL of enzyme liquid, 1 mL of NADPH solution and 1 mL of testosterone solution were added to the test tube, and shaken gently, 200 μL was taken from the 96-well enzyme-labeled plate with a pipette, three parallels were made for each sample, and placed in an enzyme-labeled instrument for detection, and the absorbance value was measured at 340 nm as the first measurement value A酶0 After the sample is placed in an incubator at 37℃ for 20 minutes, it is placed in an enzyme-labeled instrument for detection, and the absorbance value is measured at 340 nm as the second determination value A 酶20 The inhibition rate of the enzyme is calculated according to the following formula:

[0158]

[0159] The higher the 5α-reductase inhibition rate is, the stronger the oil control ability is;

[0160] The results obtained are shown in Table 3.

[0161] Table 3

[0162]

[0163] As can be seen from Table 3, when the technical solution provided by the present application is used, the obtained oil control composition has good TNF-α inhibition effect and 5α-reductase inhibition effect; specifically, the obtained oil control composition has a TNF-α inhibition rate of 30.6% or more and a 5α-reductase inhibition rate of 40.1% or more.

[0164] As can be seen from Example 1 and Comparative Examples 9-14, when other similar components are used to replace the components in the present application, the corresponding effects cannot be achieved; as can be seen from Example 1 and Comparative Examples 1-8, when any one or more of the components is not added, the obtained product cannot achieve the effect of the present application.

[0165] Effect Example 2

[0166] The application effect of the shampoo prepared in the effect example of the present application, application examples 1-16, comparative application examples 1-14 and the blank application example on the human body includes the following steps:

[0167] A certain number of male volunteers aged 18-45 years old with a certain degree of dandruff and oily scalp condition (dandruff amount ≥ 60,000 pieces, oil content ≥ 120 μg / cm 2 The dandruff amount is obtained by using a standard non-static comb to comb 100 times and using Image Pro Plus 7.0 to analyze, and the oil content is obtained by using a sebum tester Meibometer MB560). The subjects sit in a constant temperature and humidity room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes. The initial oil content is measured using a sebum tester Meibometer MB560, and the test site is the top of the head. The water probe is used to measure the water content of the scalp, and the test site is the same as the oil content test site. The initial moisture content is tested by CM 825 (Courage + Khazaka), and the test site is selected at the hairline; a professional uses a standard non-static comb to comb 60 times, collects the fallen hair and dandruff, counts the number of fallen hair, continues to comb 40 times, collects the dandruff, places the collected dandruff on a black plate, takes a photo, and analyzes the number of dandruff using Image Pro Plus 7.0. The subjects are randomly divided into 31 groups, each group of 5 people, and randomly use the shampoo provided by the application, and test the oil content, the number of hair loss, and the number of dandruff at 4 weeks, 8 weeks, and 12 weeks, analyze the improvement of the oil content, the number of hair loss, and the number of dandruff of each volunteer at 4, 8, and 12 weeks, and the improvement is indicated by the change rate of each index. The calculation method is as follows:

[0168] The change rate % relative to the initial value = | (X after use - X before use) / X before use x 100 % |; wherein the greater the change rate % relative to the initial value, the stronger the improvement effect;

[0169] Table 4

[0170]

[0171]

[0172] As can be seen from Table 4, when the technical solution provided by the application is adopted, the shampoo obtained has good comprehensive effect; specifically, it can effectively reduce oil, hair loss and dandruff, and at the same time, it can increase the water content of the primary scalp stratum corneum; at 4 weeks, the oil reduction rate, the hair loss reduction rate, and the dandruff reduction rate are more than 16.7%, 32.2%, and 22.7% respectively, and the stratum corneum water content increase rate is more than 16.3%; at 12 weeks, the oil reduction rate, the hair loss reduction rate, and the dandruff reduction rate are more than 21.3%, 43.0%, and 27.7% respectively, and the stratum corneum water content increase rate is more than 19.9%.

[0173] As can be seen from application example 1 and comparative application example, when the technical solution provided by the application is not adopted, the corresponding effect of the application cannot be achieved.

[0174] Effect example 3

[0175] The effect example of the application explores the effects of the shampoo prepared in application example 1 on male consumers, including the following steps:

[0176] 30 male subjects are selected to participate in the test, and the product is distributed to them, and each subject applies an appropriate amount of product daily for a period of 28 days of application detection, and the consumers complete the questionnaire survey of the improvement degree of the oil control effect, the dandruff effect, the itching effect, and the hair loss prevention effect. The results are shown in Table 5.

[0177] Table 5

[0178]

[0179] As can be seen from Table 5, the shampoo provided by the present application has good use experience.

[0180] It should be noted that the above examples are used to illustrate the technical solutions of the present application, but not to limit the protection scope of the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application can be modified or replaced equivalently without departing from the essence and scope of the present application.

Claims

1. A man's oil control composition comprising a Norway spruce leaf extract, characterized in that, The oil control composition comprises the following components by mass fraction: 1-3 parts of Norway spruce leaf extract, 4-6 parts of soapberry fruit extract, 0.4-0.6 parts of Schisandra chinensis extract, 0.1-0.3 parts of greyhairy milk vetch seed extract, 0.1-0.3 parts of Fraxinus muprurea bark extract, and 1-3 parts of Phellodendri cortex extract; The preparation method of the soapberry fruit extract comprises the following steps: (1) crushing soapberry fruit and adding it into deionized water, and adding cellulase and pectinase for first enzymolysis, then killing the enzyme after the first enzymolysis, and then centrifuging to collect the first enzymolysis solution; (2) adding amylase into the first enzymolysis solution for second enzymolysis, then killing the enzyme after the second enzymolysis, and then centrifuging to collect the second enzymolysis solution and concentrate and dry it to obtain the soapberry fruit extract; The mass percentage of berberine in the Phellodendri cortex extract is 2.6-4.2%.

2. The oil control composition of claim 1, wherein The mass percentage of the sum of the mass of the Norway spruce leaf extract and the mass of the greyhairy milk vetch seed extract in the total mass of the oil control composition is 14-30%.

3. The oil control composition of claim 1, wherein The mass ratio of the cellulase and the pectinase is (2-4):1; And / or, in the first enzymolysis, the mass ratio of the sum of the mass of the cellulase and the mass of the pectinase to the mass of the soapberry fruit is 1:(8-12); And / or, the temperature of the first enzymolysis is 45-55℃, and the time of the first enzymolysis is 1-3h; And / or, in the second enzymolysis, the mass ratio of the amylase to the soapberry fruit is 1:(18-22); And / or, the temperature of the second enzymolysis is 55-65℃, and the time of the second enzymolysis is 0.5-1.5h.

4. The oil control composition of claim 1, wherein The preparation method of the Phellodendri cortex extract comprises the following steps: (1) adding an emulsifier into liquid paraffin, and then adding hydrochloric acid and deionized water into the mixture and stirring to obtain a liquid membrane; (2) crushing Phellodendri cortex and adding it into deionized water for soaking, and then filtering to collect the filtrate to obtain a mother liquor; (3) adjusting the pH value of the mother liquor to 9-10, adding a liquid membrane with a volume of 20-40% of the mother liquor, and stirring, and then standing to separate layers, and collecting the liquid membrane layer; (4) heating the liquid membrane layer, standing to separate layers, collecting the aqueous phase, and concentrating and drying it to obtain the Phellodendri cortex extract.

5. The oil control composition of claim 4, wherein, In the liquid membrane, the mass percentage of the emulsifier is 2-4%, the mass percentage of the liquid paraffin is 35-45%, and the mass percentage of the hydrochloric acid is 0.5-2%; And / or, the emulsifier comprises at least one of Span-80 and Tween 80; And / or, in the step (2), the soaking temperature is 40-60℃, and the soaking time is 48-72h; And / or, in the step (3), the stirring speed is 50-100rpm, and the stirring time is 5-15min; And / or, in the step (4), the heating temperature is 80-90℃, and the heating time is 20-30min.

6. Use of the oil control composition according to any one of claims 1-5 in the preparation of a hair care product.

7. A shampoo, characterized by, The shampoo comprises the following components by mass percentage: 2-8% of the oil control composition according to any one of claims 1-5, 0.7-1.5% of a thickening agent, 15-20% of a surfactant, 0.7-1% of a preservative, 0.1-0.2% of a chelating agent, 0.1-0.5% of a pH regulator, and the balance being water.

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