Lipid-lowering weight-reducing tea as well as preparation process and application thereof

Abstract

The invention relates to the technical field of weight-reducing tea, and particularly discloses lipid-lowering weight-reducing tea as well as a preparation process and application thereof. The lipid-lowering and weight-reducing tea is prepared from rhizoma atractylodis macrocephalae, xanthoceras sorbifolia bunge, radix bupleuri, spina date seeds, echinacea purpurea, radix paeoniae alba, purpleflower holly fruits, pogostemon cablin, poria cocos, astragalus membranaceus and green tea, and a preparation process of the lipid-lowering and weight-reducing tea comprises the following steps: by taking a low cosolvent prepared from choline chloride, citric acid and water as an extraction solvent, extracting an active ingredient total flavone by a microwave method, and finally concentrating, drying, crushing and bagging to obtain the lipid-lowering and weight-reducing tea. The product has the effect of reducing hyperlipidemia; the traditional Chinese medicine composition is free of any additive and adverse reaction, is economical and practical, can relieve the economic burden of patients, and is beneficial to improving the public health level.

Description

Technical Field

[0001] The present invention relates to the field of pharmaceutical technology, and particularly relates to a lipid-lowering and weight-reducing tea and its preparation process and application. Background Art

[0002] With the change of people's dietary structure and the gradual reduction of physical activities, obesity has become a global health problem. It is closely related to the occurrence of fatty liver, hyperlipidemia and cardiovascular diseases. Dyslipidemia is characterized by an increase in low-density lipoprotein (LDL) or triglyceride (TC), and has become an important risk factor for cardiovascular diseases such as atherosclerosis and myocardial infarction; while reducing the levels of LDL and TC can significantly reduce the incidence and mortality risks of atherosclerosis. Therefore, effectively controlling dyslipidemia is of great significance for reducing and controlling the incidence of atherosclerosis among residents in China.

[0003] Currently, the measures for weight loss mainly include drug treatment, exercise and dieting. There are not many types of drugs for treating obesity, and they are expensive. They need to be taken for a long time and have relatively large side effects. Their side effects include increased blood pressure, increased heart rate, insomnia, and even teratogenicity. Incorrect dieting and exercise for weight loss can also cause other system damages or dysfunctions, and there will be a rebound after stopping. Therefore, in clinical applications, these methods are greatly limited.

[0004] Now, traditional Chinese medicine research shows that hyperlipidemia belongs to the categories of "blood stasis" and "phlegm turbidity". It is mainly because patients have improper diet and generate damp-heat, resulting in disorders of the functions of the spleen, liver and kidneys of the patients, and then leading to the failure of transportation and transformation, the inability to distinguish and secrete turbidity, and the accumulation of phlegm turbidity and fatty substances. Therefore, most clinical patients show symptoms of liver depression and spleen deficiency.

[0005] Therefore, in view of the deficiencies of the prior art, the present invention combines Chinese herbal medicines with the functions of tonifying the liver and removing dampness with tea, and provides a lipid-lowering and weight-reducing tea, which is convenient to use and has high safety, and is easily accepted by patients. Summary of the Invention

[0006] The purpose of the present invention is to provide a lipid-lowering and weight-reducing tea.

[0007] Another purpose of the present invention is to provide a preparation process of a lipid-lowering and weight-reducing tea.

[0008] Another purpose of the present invention is to provide an application of a lipid-lowering and weight-reducing tea in the preparation of lipid-lowering and weight-reducing drugs or health products.

[0009] The present invention is achieved by adopting the following technical solutions:

[0010] The lipid-lowering and weight-loss tea of ​​the present invention comprises, by weight, 20-30 parts of atractylodes macrocephala, 20-30 parts of Xanthoceras sorbifolia, 20-30 parts of bupleurum, 20-30 parts of spiny jujube seeds, 10-25 parts of echinacea, 10-25 parts of white peony root, 5-10 parts of ilex fruit, 5-20 parts of patchouli, 10-20 parts of tuckahoe, 5-20 parts of astragalus and 15-25 parts of green tea.

[0011] Furthermore, the lipid-lowering and weight-loss tea of ​​the present invention comprises, by weight, 22-28 parts of Atractylodes macrocephala, 22-28 parts of Xanthoceras sorbifolia, 22-28 parts of Bupleurum chinense, 22-28 parts of Ziziphus jujuba seeds, 14-22 parts of Echinacea purpurea, 14-22 parts of White Peony Root, 6-9 parts of Ilex versicolor, 8-16 parts of Patchouli, 12-18 parts of Poria, 8-16 parts of Astragalus, and 16-24 parts of green tea.

[0012] Furthermore, the lipid-lowering and weight-loss tea of ​​the present invention comprises, by weight, 24-26 parts of Atractylodes macrocephala, 24-26 parts of Xanthoceras sorbifolia, 24-26 parts of Bupleurum chinense, 24-26 parts of Ziziphus jujuba seeds, 16-20 parts of Echinacea purpurea, 16-20 parts of White Peony Root, 7-8 parts of Ilex versicolor, 10-14 parts of Patchouli, 14-16 parts of Poria, 10-14 parts of Astragalus, and 18-22 parts of green tea.

[0013] Furthermore, the lipid-lowering and weight-loss tea of ​​the present invention comprises, by weight, 25 parts of Atractylodes macrocephala, 25 parts of Xanthoceras sorbifolia, 25 parts of Bupleurum chinense, 25 parts of Semen Ziziphi Spinosae, 18 parts of Echinacea purpurea, 18 parts of White Peony Root, 8 parts of Ilex Verniciflua, 12 parts of Patchouli, 15 parts of Poria, 12 parts of Astragalus, and 20 parts of green tea.

[0014] The preparation process of the lipid-lowering and weight-loss tea of ​​the present invention comprises the following steps:

[0015] 1) Weigh Atractylodes macrocephala, Xanthoceras sorbifolia, Bupleurum chinense, Semen Ziziphi Spinosae, Echinacea purpurea, White Peony Root, Fructus Ilex, Poria cocos, Astragalus membranaceus, and Green Tea according to the formula ratio, grind them separately, sieve through 20-60 mesh, and mix;

[0016] 2) adding a low co-solvent to the mixed powder obtained in step 1) at a solid-liquid ratio of 1:10-20, performing microwave extraction at a power of 200-400 W for 10-20 min at an extraction temperature of 60° C., and filtering;

[0017] The low co-solvent is prepared from choline chloride, citric acid and water in a mass ratio of 1:1-5:5-10;

[0018] 3) The filtrate obtained by filtering in step 2) is concentrated into an extract, dried under reduced pressure, crushed and bagged.

[0019] Furthermore, in the preparation process of the lipid-lowering and weight-loss tea of ​​the present invention, the sieving mesh number in step 1) is 60 meshes.

[0020] Further, in the preparation process of the lipid-lowering and weight-reducing tea of the present invention, the microwave extraction in step 2) is as follows: Add the deep eutectic solvent according to a solid-liquid ratio of 1:10-15, and perform microwave extraction at a power of 300-400 W for 15-20 min.

[0021] Further, in the preparation process of the lipid-lowering and weight-reducing tea of the present invention, the microwave extraction in step 2) is as follows: Add the deep eutectic solvent according to a solid-liquid ratio of 1:15, and perform microwave extraction at a power of 300 W for 15 min.

[0022] Further, in the preparation process of the lipid-lowering and weight-reducing tea of the present invention, the deep eutectic solvent in step 2) is prepared from choline chloride, citric acid, and water according to a mass ratio of 1:3:5.

[0023] Application of the lipid-lowering and weight-reducing tea of the present invention in the preparation of lipid-lowering and weight-reducing drugs or health products.

[0024] Compared with the prior art, the present invention has the following advantages:

[0025] 1. The lipid-lowering and weight-reducing tea of the present invention is a new product and has a certain effect on reducing high blood lipid.

[0026] 2. The total flavonoid yield obtained by the extraction process of the present invention is high. The extraction of the lipid-lowering and weight-reducing tea of the present invention adopts a combination of deep eutectic solvent and microwave extraction. By comparing four extraction processes of water decoction, reflux extraction, ultrasonic extraction, and microwave method, it is found that the total flavonoid yield obtained by microwave extraction is relatively good; then, the single factors of the microwave extraction method are investigated, and combined with the orthogonal test, it is found that the composition and composition ratio of the deep eutectic solvent, water content, etc. will all affect the total flavonoid yield; when the deep eutectic solvent used in the present invention contains choline chloride and citric acid, and choline chloride and citric acid are hydrogen bond acceptors and hydrogen bond donors respectively, the total flavonoid yield is higher than that of other types of deep eutectic solvents; when the composition ratio of choline chloride and citric acid is relatively low, the hydrogen bond action and polarity of the solution system are weak, affecting the dissolution of total flavonoids; when the composition ratio of choline chloride and citric acid is too high, the intermolecular forces and other interactions between components increase, and the viscosity of the system increases, which is not conducive to the dissolution of total flavonoids; finally, the present invention selects "mass ratio 1:3:5 = choline chloride: citric acid: water" as the deep eutectic solvent, and with a solid-liquid ratio of 1:10 g / ml, an extraction time of 15 min, and an extraction power of 300 W, the total flavonoid yield is the highest under these conditions.

[0027] 3. The product of the present invention contains no any additives and has no adverse reactions. Specific embodiments

[0028] The present invention will be described in detail below with reference to specific embodiments.

[0029] Example 1 Lipid-lowering and weight-reducing tea

[0030] Formula: Atractylodes macrocephala 25g, Xanthoceras sorbifolium 25g, Bupleurum chinense 25g, Ziziphus jujuba var. spinosa 25g, Echinacea purpurea 18g, Paeonia lactiflora 18g, Ilex purpurea 8g, Pogostemon cablin 12g, Poria cocos 15g, Astragalus membranaceus 12g, Green tea 20g.

[0031] Example 2 Lipid-lowering and weight-loss tea

[0032] Formula: Atractylodes macrocephala 20g, Xanthoceras sorbifolium 20g, Bupleurum chinense 20g, Ziziphus jujuba var. spinosa 20g, Echinacea purpurea 10g, Paeonia lactiflora 10g, Ilex purpurea 5g, Pogostemon cablin 5g, Poria cocos 10g, Astragalus membranaceus 5g, Green tea 15g.

[0033] Example 3 Lipid-lowering and weight-loss tea

[0034] Formula: Atractylodes macrocephala 30g, Xanthoceras sorbifolium 30g, Bupleurum chinense 30g, Ziziphus jujuba var. spinosa 30g, Echinacea purpurea 25g, Paeonia lactiflora 25g, Ilex purpurea 10g, Pogostemon cablin 20g, Poria cocos 20g, Astragalus membranaceus 20g, Green tea 25g.

[0035] Example 4 Lipid-lowering and weight-loss tea

[0036] Formula: Atractylodes macrocephala 22g, Xanthoceras sorbifolium 22g, Bupleurum chinense 22g, Ziziphus jujuba var. spinosa 22g, Echinacea purpurea 14g, Paeonia lactiflora 14g, Ilex purpurea 6g, Pogostemon cablin 8g, Poria cocos 12g, Astragalus membranaceus 8g, Green tea 16g.

[0037] Example 5 Lipid-lowering and weight-loss tea

[0038] Formula: Atractylodes macrocephala 28g, Xanthoceras sorbifolium 28g, Bupleurum chinense 28g, Ziziphus jujuba var. spinosa 28g, Echinacea purpurea 22g, Paeonia lactiflora 22g, Ilex purpurea 9g, Pogostemon cablin 16g, Poria cocos 18g, Astragalus membranaceus 16g, Green tea 24g.

[0039] Example 6 Lipid-lowering and weight-loss tea

[0040] Formula: Atractylodes macrocephala 24g, Xanthoceras sorbifolium 24g, Bupleurum chinense 24g, Ziziphus jujuba var. spinosa 24g, Echinacea purpurea 16g, Paeonia lactiflora 6g, Ilex purpurea 7g, Pogostemon cablin 10g, Poria cocos 14g, Astragalus membranaceus 10g, Green tea 18g.

[0041] Example 7 Lipid-lowering and weight-loss tea

[0042] Formula: Atractylodes macrocephala 26g, Xanthoceras sorbifolium 26g, Bupleurum chinense 26g, Ziziphus jujuba var. spinosa 26g, Echinacea purpurea 20g, Paeonia lactiflora 20g, Ilex purpurea 8g, Pogostemon cablin 14g, Poria cocos 16g, Astragalus membranaceus 14g, Green tea 22g.

[0043] Example 8 Lipid-lowering and weight-loss tea

[0044] Formula: 25 g of Atractylodes macrocephala, 20 g of Xanthoceras sorbifolium, 25 g of Bupleurum chinense, 20 g of Ziziphus jujuba var. spinosa, 20 g of Echinacea purpurea, 20 g of Paeonia lactiflora, 5 g of Ilex purpurea, 15 g of Pogostemon cablin, 15 g of Poria cocos, 10 g of Astragalus membranaceus, 25 g of green tea.

[0045] Example 9 Lipid-lowering and weight-loss tea

[0046] Formula: 25 g of Atractylodes macrocephala, 25 g of Xanthoceras sorbifolium, 25 g of Bupleurum chinense, 25 g of Ziziphus jujuba var. spinosa, 18 g of Echinacea purpurea, 10 g of Paeonia lactiflora, 5 g of Ilex purpurea, 5 g of Pogostemon cablin, 20 g of Poria cocos, 10 g of Astragalus membranaceus, 20 g of green tea.

[0047] Example 10 Lipid-lowering and weight-loss tea

[0048] Formula: 30 g of Atractylodes macrocephala, 25 g of Xanthoceras sorbifolium, 30 g of Bupleurum chinense, 30 g of Ziziphus jujuba var. spinosa, 18 g of Echinacea purpurea, 15 g of Paeonia lactiflora, 10 g of Ilex purpurea, 10 g of Pogostemon cablin, 10 g of Poria cocos, 15 g of Astragalus membranaceus, 20 g of green tea.

[0049] Apply the formulas of the lipid-lowering and weight-loss teas in Examples 1-10 to the following preparation processes of the examples.

[0050] Preparation process of the lipid-lowering and weight-loss tea in Example 11

[0051] (1) Weigh Atractylodes macrocephala, Xanthoceras sorbifolium, Bupleurum chinense, Ziziphus jujuba var. spinosa, Echinacea purpurea, Paeonia lactiflora, Ilex purpurea, Pogostemon cablin, Poria cocos, Astragalus membranaceus, and green tea according to the formula amounts, crush them respectively, sieve through 60 mesh, and mix.

[0052] (2) Add a deep eutectic solvent to the mixed powder obtained in step (1) according to a solid-liquid ratio of 1:10, perform microwave extraction at a power of 300 W for 15 min, the extraction temperature is 60 °C, centrifuge at a rotation speed of 5000 r / min for 15 min, and filter; the deep eutectic solvent is prepared from choline chloride, citric acid, and water according to a mass ratio of 1:3:5.

[0053] (3) Concentrate the filtrate obtained by filtering in step (2) into a thick paste, perform vacuum drying at a temperature of 60 °C and a vacuum degree of -0.07 to -0.09 MPa, and crush and bag it.

[0054] Preparation process of the lipid-lowering and weight-loss tea in Example 12

[0055] (1) Weigh Atractylodes macrocephala, Xanthoceras sorbifolium, Bupleurum chinense, Ziziphus jujuba var. spinosa, Echinacea purpurea, Paeonia lactiflora, Ilex purpurea, Pogostemon cablin, Poria cocos, Astragalus membranaceus, and green tea according to the formula amounts, crush them respectively, sieve through 60 mesh, and mix.

[0056] (2) Add the deep eutectic solvent to the mixed powder obtained in step (1) at a solid-liquid ratio of 1:15, perform microwave extraction at a power of 400 W for 10 min, with the extraction temperature being 60 °C, centrifuge at a rotational speed of 5000 r / min for 15 min, and then filter; the deep eutectic solvent is prepared from choline chloride, citric acid, and water at a mass ratio of 1:3:5;

[0057] (3) Concentrate the filtrate obtained by filtering in step (2) into a thick paste, perform vacuum drying at a temperature of 60 °C and a vacuum degree of -0.07 to -0.09 MPa, and then pulverize and bag it.

[0058] Preparation process of the lipid-lowering and weight-reducing tea in Example 13

[0059] (1) Weigh atractylodes macrocephala, xanthoceras sorbifolia, bupleurum chinense, ziziphus jujuba var. spinosa, echinacea purpurea, white peony root, ilicium virgatum, pogostemon cablin, poria cocos, astragalus membranaceus, and green tea according to the formula amounts, pulverize them respectively, sieve through 60 meshes, and mix them;

[0060] (2) Add the deep eutectic solvent to the mixed powder obtained in step (1) at a solid-liquid ratio of 1:20, perform microwave extraction at a power of 200 W for 20 min, with the extraction temperature being 60 °C, centrifuge at a rotational speed of 5000 r / min for 15 min, and then filter; the deep eutectic solvent is prepared from choline chloride, citric acid, and water at a mass ratio of 1:3:5;

[0061] (3) Concentrate the filtrate obtained by filtering in step (2) into a thick paste, perform vacuum drying at a temperature of 60 °C and a vacuum degree of -0.07 to -0.09 MPa, and then pulverize and bag it.

[0062] Preparation process of the lipid-lowering and weight-reducing tea in Example 14

[0063] (1) Weigh atractylodes macrocephala, xanthoceras sorbifolia, bupleurum chinense, ziziphus jujuba var. spinosa, echinacea purpurea, white peony root, ilicium virgatum, pogostemon cablin, poria cocos, astragalus membranaceus, and green tea according to the formula amounts, pulverize them respectively, sieve through 40 meshes, and mix them;

[0064] (2) Add the deep eutectic solvent to the mixed powder obtained in step (1) at a solid-liquid ratio of 1:10, perform microwave extraction at a power of 300 W for 15 min, with the extraction temperature being 60 °C, centrifuge at a rotational speed of 5000 r / min for 15 min, and then filter; the deep eutectic solvent is prepared from choline chloride, citric acid, and water at a mass ratio of 1:1:5;

[0065] (3) Concentrate the filtrate obtained by filtering in step (2) into a thick paste, perform vacuum drying at a temperature of 60 °C and a vacuum degree of -0.07 to -0.09 MPa, and then pulverize and bag it.

[0066] Preparation process of the lipid-lowering and weight-reducing tea in Example 14

[0067] (1) Weigh atractylodes macrocephala, xanthoceras sorbifolia, bupleurum chinense, ziziphus jujuba var. spinosa, echinacea purpurea, white peony root, ilicis purpureae fructus, pogostemon cablin, poria cocos, astragalus membranaceus, and green tea according to the formula amounts, crush them separately, sieve through 20 mesh, and mix them;

[0068] (2) Add the deep eutectic solvent to the mixed powder obtained in step (1) at a material-liquid ratio of 1:10, perform microwave extraction at a power of 300 W for 15 min, with the extraction temperature being 60 °C, centrifuge at a rotation speed of 5000 r / min for 15 min, and filter;

[0069] The deep eutectic solvent is prepared from choline chloride, citric acid, and water in a mass ratio of 1:5:10;

[0070] (3) Concentrate the filtrate obtained by filtering in step (2) into a thick paste, perform vacuum drying at a temperature of 60 °C and a vacuum degree of -0.07 to -0.09 MPa, and crush and bag it.

[0071] To further verify the feasibility of the present invention, the inventor conducted a series of experiments as follows:

[0072] I. Process investigation

[0073] 1.1 Screening of extraction methods

[0074] Formula of lipid-lowering and weight-loss tea: 25 g of atractylodes macrocephala, 25 g of xanthoceras sorbifolia, 25 g of bupleurum chinense, 25 g of ziziphus jujuba var. spinosa, 18 g of echinacea purpurea, 18 g of white peony root, 8 g of ilicis purpureae fructus, 12 g of pogostemon cablin, 15 g of poria cocos, 12 g of astragalus membranaceus, and 20 g of green tea. The lipid-lowering and weight-loss tea of the present invention contains active ingredients such as total flavonoids, amino acids, proteins, polysaccharides, and anthraquinones. In this study, total flavonoids were mainly used as the index, and the process was screened and investigated by a single extraction method as follows:

[0075] (1) Decoction method

[0076] Take 25 g of atractylodes macrocephala, 25 g of xanthoceras sorbifolia, 25 g of bupleurum chinense, 25 g of ziziphus jujuba var. spinosa, 18 g of echinacea purpurea, 18 g of white peony root, 8 g of ilicis purpureae fructus, 12 g of pogostemon cablin, 15 g of poria cocos, 12 g of astragalus membranaceus, and 20 g of green tea, crush them separately through a 60-mesh sieve, add 10 times the amount of water, decoct for 1.5 hours, filter, concentrate the filtrate and make up the volume to 500 ml, and determine the total flavonoid content.

[0077] (2) Reflux extraction method

[0078] Take 25 g of atractylodes macrocephala, 25 g of xanthoceras sorbifolia, 25 g of bupleurum chinense, 25 g of ziziphus jujuba var. spinosa, 18 g of echinacea purpurea, 18 g of white peony root, 8 g of ilicis purpureae fructus, 12 g of pogostemon cablin, 15 g of poria cocos, 12 g of astragalus membranaceus, and 20 g of green tea, crush them separately through a 60-mesh sieve, add 10 times the amount of 60% ethanol, heat and reflux for 1.5 hours, filter, concentrate the filtrate and make up the volume to 500 ml, and determine the total flavonoid content.

[0079] (3) Ultrasonic method

[0080] Take 25 g of Atractylodes macrocephala, 25 g of Xanthoceras sorbifolium, 25 g of Bupleurum chinense, 25 g of Ziziphus jujuba var. spinosa, 18 g of Echinacea purpurea, 18 g of Paeonia lactiflora, 8 g of Ilex purpurea, 12 g of Pogostemon cablin, 15 g of Poria cocos, 12 g of Astragalus membranaceus, and 20 g of green tea. Crush them separately through a 60-mesh sieve, add 10 times the amount of 60% ethanol, extract by ultrasonic wave for 1.5 hours, filter, concentrate the filtrate and make the volume constant to 500 ml, and determine the total flavonoid content.

[0081] (4) Microwave method

[0082] Take 25 g of Atractylodes macrocephala, 25 g of Xanthoceras sorbifolium, 25 g of Bupleurum chinense, 25 g of Ziziphus jujuba var. spinosa, 18 g of Echinacea purpurea, 18 g of Paeonia lactiflora, 8 g of Ilex purpurea, 12 g of Pogostemon cablin, 15 g of Poria cocos, 12 g of Astragalus membranaceus, and 20 g of green tea. Crush them separately through a 60-mesh sieve, add 10 times the amount of 60% ethanol, extract by microwave at a power of 200 W for 15 min, the extraction temperature is 60 °C, centrifuge and filter, concentrate the filtrate and make the volume constant to 500 ml, and determine the total flavonoid content.

[0083] 1.2 Determination method of total flavonoid content

[0084] The ultraviolet spectrophotometry was used for determination. In the experiment, rutin was used as the standard product, the NaNO2-Al(NO3)3-NaOH colorimetric method was adopted, and 510 nm was selected as the detection wavelength to determine the total flavonoid in the lipid-lowering and weight-reducing tea.

[0085] Accurately weigh 20 mg of rutin, dissolve it by ultrasonic wave with 70% ethanol, transfer it to a 100-ml volumetric flask and make the volume constant to prepare a rutin standard solution with a concentration of 0.2 mg / ml.

[0086] Absorb 0 ml, 1 ml, 2 ml, 3 ml, 4 ml, 5 ml of the rutin reference solution and place them in 25-ml volumetric flasks. Then add 70% ethanol respectively to make the total volume reach 5 ml. Add 1 ml of 5% NaNO2 solution again, shake well, let stand for 6 min, add 1 ml of 10% Al(NO3)3 solution, shake well, let stand for 6 min, then add 10 ml of 4% NaOH solution, make the volume constant to the scale with distilled water, shake well, let stand for 15 min, use it as a blank control, measure the absorbance at a wavelength of 510 nm, take the rutin mass concentration as the abscissa and the absorbance as the ordinate, draw the standard curve and fit to obtain the regression equation.

[0087] Take 5 ml of the test solution and replace the rutin reference solution with it, and measure the absorbance according to the above method. Substitute the absorbance value into the standard curve to calculate the concentration of total flavonoid in the sample solution.

[0088] The results of determining the total flavonoid are shown in Table 1.

[0089] Table 1 Total flavonoid yields of extracts by different extraction methods

[0090] Extraction method Water decoction method Reflux extraction method Ultrasonic method Microwave method Total flavonoid content (%) 2.15 2.82 2.47 4.91

[0091] As can be seen from Table 1, the total flavonoid content is the highest by the microwave method, followed by the reflux extraction method, and the lowest by the ultrasonic method. Therefore, microwave extraction was considered in this study.

[0092] 1.3 Single-factor experiments of the microwave method

[0093] 1.3.1 Effect of extraction solvent on the yield of total flavonoids

[0094] On the basis of the above microwave method, the effects of different solvents on the yield of total flavonoids were investigated respectively.

[0095] It can be seen from Table 2 that when the deep eutectic solvent is used for extraction, the yield of total flavonoids is higher than that when ethanol is used as the solvent; choline chloride:citric acid:water (1:3:5) > choline chloride:citric acid:water (1:1:5) > betaine:citric acid (1:5) > choline chloride:uric acid (1:2) > 60% ethanol > ethanol; it is found that the composition of the deep eutectic solvent, the composition ratio between components, the water content, etc. will all affect the extraction yield of total flavonoids; when the deep eutectic solvent contains choline chloride and citric acid, and choline chloride and citric acid are the hydrogen bond acceptor and hydrogen bond donor respectively, the yield of total flavonoids is higher than that of other types of deep eutectic solvents; when the composition ratio of choline chloride and citric acid is low, the hydrogen bond interaction and polarity of the solution system are weak, affecting the dissolution of total flavonoids; when the composition ratio of choline chloride and citric acid is too high, the intermolecular forces and other interactions between components increase, and the system viscosity increases, which is not conducive to the dissolution of total flavonoids; therefore, the deep eutectic solvent "choline chloride:citric acid:water (1:3:5)" was selected as the extraction solvent.

[0096] Table 2 Effect of different solvents on the yield of total flavonoids

[0097]

[0098] 1.3.2 Effect of solid-liquid ratio on the yield of total flavonoids

[0099] According to the determination method under the above "1.3.1", the effects of different solid-liquid ratios on the yield of total flavonoids were investigated respectively.

[0100] It can be seen from Table 3 that as the solid-liquid ratio increases, the yield of total flavonoids gradually increases. When the solid-liquid ratio is 1:10, the yield tends to be flat and the increase is not obvious. Therefore, the solid-liquid ratio of "1:10" was selected.

[0101] Table 3 Effect of different solid-liquid ratios on the yield of total flavonoids

[0102] Solid-liquid ratio (g / ml) 1:5 1:10 1:15 1:20 1:25 Total flavonoid content (%) 5.03 5.11 5.74 5.79 5.80

[0103] 1.3.3 Effect of extraction time on the yield of total flavonoids

[0104] According to the determination method under the above "1.3.2", the effects of different extraction times on the yield of total flavonoids were investigated respectively.

[0105] As can be seen from Table 4, with the increase of microwave extraction time, the yield of total flavonoids continuously increases. When the extraction time is 15 minutes, the yield tends to be stable. Therefore, the extraction time of "15 minutes" was selected.

[0106] Table 4 Effects of different extraction times on the yield of total flavonoids

[0107] Extraction time (min) 5 10 15 20 25 Total flavonoid content (%) 4.97 5.60 5.84 5.88 5.91

[0108] 1.3.4 Effect of microwave power on the yield of total flavonoids

[0109] According to the determination method under the above "1.3.3", the effects of different microwave powers on the yield of total flavonoids were investigated respectively.

[0110] As can be seen from Table 5, with the increase of microwave power, the yield of total flavonoids continuously increases. However, when the microwave power reaches 400 W, the yield decreases instead. Therefore, the microwave power of "300 W" was selected.

[0111] Table 5 Effects of different microwave powers on the yield of total flavonoids

[0112] Microwave power (W) 100 200 300 400 500 Total flavonoid content (%) 5.02 5.84 5.99 5.98 5.93

[0113] 1.4 Orthogonal experiment design

[0114] On the basis of the above single-factor investigation, in this experiment, the eutectic solvent ratio (A), solid-liquid ratio (B), extraction time (C), and extraction power (D) were selected as the investigation factors, and the orthogonal test method of four factors and three levels of L9(3 4 ) was used to optimize the process conditions. The yield of total flavonoids was used as the evaluation index. The factor levels are shown in Table 6, the orthogonal test results are shown in Table 7, and the variance analysis is shown in Table 8.

[0115] Table 6 Factor level table of orthogonal experiment

[0116]

[0117] Table 7 Orthogonal test result table

[0118]

[0119]

[0120] Table 8 Variance analysis of yield results

[0121]

[0122] From the intuitive analysis of the orthogonal experiment in Table 7, the order of the influence of each factor on the total flavonoid yield is factor A > C > B > D, and the optimal extraction process is A2B1C2D2; from the extreme value K and the range R, it can be seen that the mass ratio of the deep eutectic solvent choline chloride:citric acid:water has an impact on the total flavonoid yield, while the solid-liquid ratio, extraction time, and power have no significant effect on the total flavonoid yield. Considering factors such as cost, the optimal extraction process A2B1C2D2 is finally determined, that is, the mass ratio 1:3:5 = choline chloride:citric acid:water is selected as the deep eutectic solvent, the solid-liquid ratio is 1:10 g / ml, the extraction time is 15 min, and the extraction power is 300 W.

[0123] 1.5 Verification of the optimal preparation process

[0124] Prepare 3 batches of samples according to the optimal preparation process obtained from the experiment, and determine the total flavonoid yield. The test results are shown in Table 9.

[0125] Table 9 Results of the verification test

[0126]

[0127] Results: The total flavonoid yields of the 3 batches of samples prepared according to the optimal preparation process of the present invention are 6.04%, 5.95%, and 5.88% respectively, with an average value of 5.96% and RSD = 1.35%, indicating that this method is stable and feasible.

[0128] 1.6 Process determination

[0129] Take 25 g of Atractylodes macrocephala, 25 g of Xanthoceras sorbifolia, 25 g of Bupleurum chinense, 25 g of Ziziphus jujuba var. spinosa, 18 g of Echinacea purpurea, 18 g of Paeonia lactiflora, 8 g of Ilex purpurea, 12 g of Pogostemon cablin, 15 g of Poria cocos, 12 g of Astragalus membranaceus, and 20 g of green tea, crush them respectively through a 60-mesh sieve, add 10 times the amount of the deep eutectic solvent composed of choline chloride:citric acid:water (1:3:5), carry out microwave extraction at a power of 300 W for 15 min, the extraction temperature is 60 °C, centrifuge and filter, concentrate the filtrate into an extract, dry it under reduced pressure, and powder and bag it to obtain the product.

[0130] II. Animal experiments

[0131] 2 Establish a hyperlipidemic obese rat model

[0132] Use the SD rat obesity model to evaluate the lipid-lowering effect of the lipid-lowering and weight-reducing tea.

[0133] 2.1 Experimental animals

[0134] Forty-eight male SD rats, weighing 100 - 120 g. They are divided into 6 groups, namely the normal group, the model group, the positive drug group, the high-dose lipid-lowering and weight-reducing tea group, the medium-dose lipid-lowering and weight-reducing tea group, and the low-dose lipid-lowering and weight-reducing tea group, with 8 rats in each group.

[0135] 2.2 Methods

[0136] (1) Drug preparation

[0137] High-fat diet: 83% basal diet + 5% cholesterol + 2% sucrose + 10% lard;

[0138] Positive drug: Pravastatin, 8.6 mg / Kg;

[0139] High-dose group of lipid-lowering and weight-reducing tea: 1.5 g / Kg;

[0140] Medium-dose group of lipid-lowering and weight-reducing tea: 1.0 g / Kg;

[0141] Low-dose group of lipid-lowering and weight-reducing tea: 0.5 g / Kg;

[0142] (2) Model establishment and drug administration

[0143] Except for the normal group, the other 5 groups were fed with high-fat diet for 8 weeks. Four indicators, namely TG (triglyceride), TC (total serum cholesterol), HDL (high-density lipoprotein), and LDL (low-density lipoprotein), were examined. After the successful establishment of the hyperlipidemia model, drug administration was started for 4 weeks, once a day at the same time, administered at 1 ml / 100 g. During drug administration, the high-fat diet was continued until the end of drug administration.

[0144] (3) Detection indicators

[0145] Rats were anesthetized with ether, blood was collected from the orbital venous plexus, centrifuged for 15 min to prepare serum, which was stored at -20 °C in the refrigerator after being aliquoted. Four indicators were examined using TG, TC, HDL, and LDL assay kits.

[0146] (4) Statistical methods

[0147] SPSS 12.0 software was used to analyze and statistically process the experimental data. The t-test was used for comparison between groups, and P < 0.05 was considered statistically significant.

[0148] 2.3 Results

[0149] (1) Construction of hyperlipidemia model

[0150] Results: The contents of TG and TC were significantly increased compared with the normal group (P < 0.01), the content of LDL was significantly increased compared with the normal group (P < 0.01), and the content of HDL was significantly decreased compared with the normal group (P < 0.01). The detection results of the four indicators showed that the hyperlipidemia model was successfully constructed.

[0151] (2) Effects of lipid-lowering and weight-reducing tea on TG, TC, HDL, and LDL in hyperlipidemic model rats

[0152] Results: Compared with the normal group, the contents of serum TG, TC, and LDL in the model group rats were significantly increased (P < 0.01), and the content of HDL was significantly decreased (P < 0.01); compared with the model group, the contents of serum TG, TC, and LDL in the positive drug group rats were significantly decreased (P < 0.01), and the content of serum HDL was significantly increased (P < 0.01), with statistically significant differences; in the high-dose group rats, the contents of serum TG and TC were significantly decreased, the content of LDL was extremely significantly decreased (P < 0.001), and the content of HDL was extremely significantly increased (P < 0.001), with statistically significant differences; in the medium-dose group rats, the contents of serum TG and TC were slightly decreased, the content of LDL had a decreasing trend (P > 0.05), and the content of HDL had an increasing trend (P > 0.05), but the differences were not statistically significant; in the low-dose group rats, there were no obvious differences in the four indexes of serum TG, TC, HDL, and LDL (P > 0.05), without statistical significance; therefore, the high-dose group of the lipid-lowering and weight-reducing tea had a significant lipid-lowering effect.

[0153] III. Clinical Efficacy Evaluation

[0154] Evaluate the clinical efficacy and safety of the lipid-lowering and weight-reducing tea product obtained by the preparation method of Example 11 according to the formula of Example 1 of the present application in the treatment of hyperlipidemia.

[0155] 3.1 General Requirements for Volunteers

[0156] Volunteers are required to provide a physical examination report within 1 month before participation. At least one of the four blood lipids, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL), and low-density lipoprotein cholesterol (LDL), of the patients exceeds the following standards:

[0157] (1) TG: > 1.70 mmol / L;

[0158] (2) TC: > 5.72 mmol / L;

[0159] (3) HDL: < 1.04 mmol / L;

[0160] (4) LDL: > 3.12 mmol / L.

[0161] 3.2 Grouping and Treatment Methods

[0162] There were 42 participants in the experimental group, divided into four groups. Among them, the first group had abnormal TG: 8 people; the second group had abnormal TC: 12 people; the third group had abnormal HDL: 15 people; the fourth group had abnormal LDL: 7 people. The trial period was 8 weeks. The lipid-lowering and weight-loss tea was taken twice a day, brewed with boiling water, 1 bag / time, 75g / bag. During this period, corresponding health services and life status tracking were provided. The changes in the main indicators of triglyceride, total cholesterol, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol before and after taking the tea by the experiencers were collected.

[0163] 3.3 Efficacy Criteria

[0164] (1) Cured: Blood lipid returned to normal value;

[0165] (2) Effective: The change in blood lipid was significant compared with that before taking the medicine and was close to the normal value;

[0166] (3) Ineffective: No obvious change in blood lipid.

[0167] 3.4 Results

[0168] Among the 42 volunteers, 12 were cured, 29 were effective, and 1 was ineffective. The total effective rate was 97.6%. See Table 10.

[0169] Table 10 Statistics of the Total Effective Rate after Treatment

[0170]

[0171] 3.4 Typical Cases

[0172] Case 1, Patient Zhao xx, male, 52 years old. Physical examination: Serum total cholesterol 6.05 mmol / L, serum triglyceride 2.41 mmol / L. Diagnosis: Hyperlipidemia.

[0173] The patient brewed one bag (75g / bag) of the lipid-lowering and weight-loss tea with boiling water after lunch and dinner every day for 64 days. After testing: Serum total cholesterol 5.5 mmol / L, serum triglyceride 1.58 mmol / L.

[0174] Case 2, Patient Lv xx, female, 45 years old. Physical examination: Low-density lipoprotein cholesterol 4.39 mmol / L. Diagnosis: Hyperlipidemia.

[0175] The patient brewed one bag (75g / bag) of the lipid-lowering and weight-loss tea with boiling water after lunch and dinner every day for 60 days. After testing: Low-density lipoprotein cholesterol 2.32 mmol / L.

[0176] The embodiments described in the present invention are merely descriptions of the preferred embodiments of the present invention, and do not limit the concept and scope of the present invention. Without departing from the design concept of the present invention, various modifications and improvements made by those skilled in the art to the technical solutions of the present invention shall fall within the protection scope of the present invention.

Claims

1. A lipid-lowering and weight-loss tea, characterized in that, By weight parts, it consists of 20 - 30 parts of Atractylodes macrocephala, 20 - 30 parts of Xanthoceras sorbifolium, 20 - 30 parts of Bupleurum chinense, 20 - 30 parts of Ziziphus jujuba var. spinosa, 10 - 25 parts of Echinacea purpurea, 10 - 25 parts of Paeonia lactiflora, 5 - 10 parts of Ilex purpurea, 5 - 20 parts of Pogostemon cablin, 10 - 20 parts of Poria cocos, 5 - 20 parts of Astragalus membranaceus, and 15 - 25 parts of green tea.

2. A lipid-lowering and weight-reducing tea, characterized in that, By weight parts, it consists of 22 - 28 parts of Atractylodes macrocephala, 22 - 28 parts of Xanthoceras sorbifolium, 22 - 28 parts of Bupleurum chinense, 22 - 28 parts of Ziziphus jujuba var. spinosa, 14 - 22 parts of Echinacea purpurea, 14 - 22 parts of Paeonia lactiflora, 6 - 9 parts of Ilex purpurea, 8 - 16 parts of Pogostemon cablin, 12 - 18 parts of Poria cocos, 8 - 16 parts of Astragalus membranaceus, and 16 - 24 parts of green tea.

3. A lipid-lowering and weight-reducing tea, characterized in that, By weight parts, it consists of 24 - 26 parts of Atractylodes macrocephala, 24 - 26 parts of Xanthoceras sorbifolium, 24 - 26 parts of Bupleurum chinense, 24 - 26 parts of Ziziphus jujuba var. spinosa, 16 - 20 parts of Echinacea purpurea, 16 - 20 parts of Paeonia lactiflora, 7 - 8 parts of Ilex purpurea, 10 - 14 parts of Pogostemon cablin, 14 - 16 parts of Poria cocos, 10 - 14 parts of Astragalus membranaceus, and 18 - 22 parts of green tea.

4. A lipid-lowering and weight-loss tea, characterized in that, By weight parts, it consists of 25 parts of Atractylodes macrocephala, 25 parts of Xanthoceras sorbifolium, 25 parts of Bupleurum chinense, 25 parts of Ziziphus jujuba var. spinosa, 18 parts of Echinacea purpurea, 18 parts of Paeonia lactiflora, 8 parts of Ilex purpurea, 12 parts of Pogostemon cablin, 15 parts of Poria cocos, 12 parts of Astragalus membranaceus, and 20 parts of green tea.

5. The preparation process of the lipid-lowering and weight-reducing tea according to any one of claims 1-4, characterized in that, It includes the following steps: 1) Weigh Atractylodes macrocephala, Xanthoceras sorbifolium, Bupleurum chinense, Ziziphus jujuba var. spinosa, Echinacea purpurea, Paeonia lactiflora, Ilex purpurea, Pogostemon cablin, Poria cocos, Astragalus membranaceus, and green tea according to the formula ratio, crush them respectively, sieve through 20 - 60 meshes, and mix. 2) Add a deep eutectic solvent to the mixed powder obtained in step 1) according to a solid - liquid ratio of 1:10 - 20, carry out microwave extraction at a power of 200 - 400 W for 10 - 20 min, with the extraction temperature being 60 °C, and filter. The deep eutectic solvent is prepared from choline chloride, citric acid, and water according to a mass ratio of 1:1 - 5:5 - 10. 3) Concentrate the filtrate obtained by filtering in step 2) into an extract, carry out vacuum drying, crush and pack.

6. The preparation process of the lipid-lowering and weight-reducing tea according to claim 5, characterized in that, The sieve mesh number in step 1) is 60 meshes.

7. The preparation process of the lipid-lowering and weight-reducing tea according to claim 5, characterized in that, The microwave extraction in step 2) is as follows: Add a deep eutectic solvent according to a solid - liquid ratio of 1:10 - 15, and carry out microwave extraction at a power of 300 - 400 W for 15 - 20 min.

8. The preparation process of the lipid-lowering and weight-reducing tea according to claim 7, characterized in that, The microwave extraction in step 2) is as follows: Add a deep eutectic solvent according to a solid - liquid ratio of 1:10, and carry out microwave extraction at a power of 300 W for 15 min.

9. The preparation process of the lipid-lowering and weight-reducing tea according to claim 5, characterized in that, The deep eutectic solvent in step 2) is prepared from choline chloride, citric acid, and water according to a mass ratio of 1:3:

5.

10. Use of the lipid - lowering and weight - reducing tea according to any one of claims 1 - 4 in the preparation of lipid - lowering and weight - reducing drugs or health products.

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