Traditional Chinese medicine preparation based on green tea, preparation method of traditional Chinese medicine preparation and application of traditional Chinese medicine preparation in preparation of green tea drink for multi-target regulation of glycolipid metabolism

Abstract

The invention discloses a traditional Chinese medicine preparation based on green tea, a preparation method of the traditional Chinese medicine preparation and application of the traditional Chinese medicine preparation to preparation of a green tea drink for multi-target regulation of glycolipid metabolism. The formula of the green tea drink comprises 45-55 parts of green tea, 15-20 parts of lotus leaves, 15-20 parts of mulberry leaves and 15-20 parts of raspberries. The traditional Chinese medicine preparation can effectively relieve liver gluconeogenesis caused by hyperglycemia, improve hormone and signal molecule disorder caused by high glucose and high fat and reduce hyperlipidemia caused by high fat diet. The preparation method disclosed by the invention is scientific and reasonable, can effectively retain the active ingredients of the green tea and improve the bioavailability of the green tea, has a good auxiliary effect of reducing blood fat and blood sugar, is suitable for daily drinking of people with diabetes and dyslipidemia, and has a wide application prospect.

Description

Technical Field

[0001] The invention relates to a Chinese medicine preparation based on green tea, a preparation method thereof and application thereof in preparing a green tea beverage for regulating glucose and lipid metabolism at multiple targets, and belongs to the field of tea beverages. Background Art

[0002] With the changes in lifestyle and dietary structure, the incidence of metabolic diseases such as hyperlipidemia and hyperglycemia caused by factors such as high-fat diet has increased year by year, seriously threatening human health and becoming an important public health issue worldwide. Studies have shown that long-term high-fat diet can induce a series of pathological processes such as insulin resistance, adipose tissue dysfunction and abnormal liver gluconeogenesis, which in turn lead to metabolic syndrome manifestations such as imbalance of glucose and lipid metabolism and abnormal endocrine hormone levels. Clinically, the rat model induced by streptozotocin (STZ) combined with high-fat diet has been widely used to simulate human high-fat and high-sugar related metabolic diseases, and has become a commonly used model for studying hypoglycemic and lipid-lowering drugs and functional foods.

[0003] Although a variety of oral hypoglycemic and lipid-lowering drugs are widely used, their long-term use may lead to side effects, and most of them mainly act on a single target, making it difficult to fully regulate complex metabolic pathways. Therefore, the development of functional foods with natural sources, mild effects, high safety, and multi-target intervention in metabolic diseases has become a research hotspot. Among them, green tea, as a representative drink among teas, is rich in active ingredients such as tea polyphenols, caffeine, and catechins, and has good physiological functions such as antioxidant, anti-inflammatory, improving insulin sensitivity, and regulating lipid metabolism. In recent years, more and more studies have focused on the synergistic application of green tea and medicinal and edible plants, such as lotus leaves, mulberry leaves, hawthorn, raspberries, etc. These natural substances are widely used in traditional medicine to regulate abnormal glucose and lipid metabolism caused by "phlegm dampness", "blood stasis", and "yin deficiency". Modern research has also confirmed that they can play an important role in regulating liver gluconeogenesis and hormone secretion.

[0004] However, most existing tea beverages lack targeted functional design, and it is difficult to take into account the regulatory effects of multiple links in glucose and lipid metabolism. How to scientifically combine green tea with synergistic medicinal and edible ingredients, and effectively intervene in the core metabolic pathway of "liver gluconeogenesis-lipid metabolism-hormone balance" in terms of functional mechanism, is still a key issue in the current research and development of functional tea beverages. To this end, it is urgent to develop a new multi-target metabolic regulation green tea beverage product with a clear physiological mechanism basis and proven effective in animal experiments, in order to provide a safer and more effective dietary intervention plan for people with hyperglycemia and hyperlipidemia. Summary of the invention

[0005] Objective of the Invention: The objective of the present invention is to provide a traditional Chinese medicine preparation based on green tea, its preparation method, and its application in preparing a green tea beverage for multi-target regulation of glycolipid metabolism.

[0006] Technical Solution: The present invention provides a traditional Chinese medicine preparation based on green tea, which is prepared from the following components in parts by weight: 45-55 parts of green tea, 15-20 parts of lotus leaf, 15-20 parts of mulberry leaf, and 15-20 parts of raspberry.

[0007] Further, the formula of the traditional Chinese medicine preparation includes 50 parts of green tea, 16 parts of lotus leaf, 16 parts of mulberry leaf, and 16 parts of raspberry.

[0008] Further, the green tea includes Tieguanyin or Anji white tea.

[0009] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine preparation based on green tea, which includes the following steps: (1) removing impurities and washing the lotus leaf, mulberry leaf, and raspberry, and then performing hot air drying; (2) mixing the dried lotus leaf, mulberry leaf, raspberry with green tea in proportion, and performing high-speed pulverization, a total of 4-5 times; (3) formulating the pulverized composition according to the solid-liquid ratio, and performing ultrasonic extraction.

[0010] Further, in step (1), the drying temperature is 100-120 °C, and the time is 10-20 min.

[0011] Further, in step (2), the rotation speed of the high-speed pulverization is 3000-4000 revolutions per minute, and each pulverization is 10-20 s.

[0012] Further, in step (3), the solid-liquid ratio is 1:50-200; the ultrasonic conditions are extraction at 30-50 °C for 60-100 min.

[0013] Further, the multi-target regulation of glycolipid metabolism includes alleviating hepatic gluconeogenesis caused by hyperglycemia, improving the disorder of hormones and signaling molecules caused by high sugar and high fat, and reducing hyperlipidemia caused by high-fat diet.

[0014] The present invention also provides the application of the above-mentioned traditional Chinese medicine preparation based on green tea in preparing a green tea beverage for multi-target regulation of glycolipid metabolism. The alleviation of hepatic gluconeogenesis caused by hyperglycemia includes regulating the levels of glycogen, pyruvate, and lactate in the liver.

[0015] Further, the improvement of the disorder of hormones and signaling molecules caused by high sugar and high fat includes regulating the levels of insulin, insulin-like growth factor-1, glucagon-like peptide-1, and C-peptide.

[0016] Further, the reduction of hyperlipidemia caused by high-fat diet includes reducing the levels of total protein, total cholesterol, and triglyceride in the serum.

[0017] The above-mentioned green tea beverage contains rich bioactive substances, which contribute to the recovery of hyperglycemia caused by streptozotocin (STZ) and high-fat diet. The following is a detailed introduction to the functions of each component:

[0018] Tieguanyin tea is rich in various active ingredients, including tea polyphenols, catechins, caffeine, amino acids, flavonoid compounds, and various vitamins and minerals. It has the functions of antioxidant, anti-inflammatory, lipid-lowering, blood sugar regulation, immune enhancement, and can also promote fat decomposition, contributing to weight loss and improving digestive function.

[0019] Lotus leaf is rich in flavonoid compounds, quercetin, nuciferine, protocatechuic acid, alkaloids, polysaccharides, and vitamin C, etc. It has the functions of lipid-lowering, blood pressure-lowering, blood sugar regulation, antioxidant, anti-inflammatory, diuretic, etc., and can promote fat metabolism, contributing to weight loss and prevention of obesity. Nuciferine has a particularly significant effect on the regulation of blood lipid and cholesterol, while flavonoid compounds contribute to cardiovascular protection.

[0020] Mulberry leaf is rich in flavonoid compounds (quercetin, rutin), polysaccharides, 1-deoxynojirimycin (DNJ), alkaloids, amino acids, and trace elements. It has the functions of blood sugar-lowering, lipid-lowering, antioxidant, anti-inflammatory, antibacterial, etc. Especially, DNJ has a unique effect on blood sugar regulation in inhibiting the activity of α-glucosidase. In addition, mulberry leaf polysaccharides can enhance immune function, and flavonoid compounds contribute to cardiovascular protection.

[0021] Raspberry is rich in active ingredients such as anthocyanins, flavonoid compounds, ellagic acid, vitamin C, quercetin, polyphenols, and fatty acids, and has various health benefits. It can regulate blood sugar, lower blood lipid, and prevent cardiovascular diseases. Anthocyanins and polyphenols help to scavenge free radicals and protect cells from oxidative stress damage.

[0022] Among them, lotus leaf, mulberry leaf, and raspberry all belong to the substances that are both foods and Chinese medicinal materials as approved by the Ministry of Health of the People's Republic of China.

[0023] The four natural plants of Tieguanyin tea, lotus leaf, mulberry leaf, and raspberry have a synergistic effect in traditional Chinese medicine theory and there is strong evidence for their use in conditioning hyperglycemia and hyperlipidemia. Tieguanyin tea clears heat and reduces lipid, lotus leaf resolves dampness and lowers turbidity, mulberry leaf clears the liver and moistens the lungs, generates fluid and quenches thirst, while raspberry tonifies the kidney and consolidates essence. The combination of the four can take into account clearing heat and resolving dampness and tonifying the kidney and strengthening the root, and condition the abnormal glucose and lipid metabolism caused by "spleen deficiency and dampness accumulation" and "liver and kidney deficiency", which conforms to the traditional Chinese medicine treatment principle of "treating both the symptoms and the root causes".

[0024] Modern pharmacological studies have also verified its potential for reducing blood sugar and lipid levels: Tieguanyin is rich in tea polyphenols, which can improve insulin resistance. The active ingredients in lotus leaf and mulberry leaf can inhibit sugar absorption and promote lipid metabolism. Raspberry is rich in components such as anthocyanins, which can have antioxidant effects and improve metabolic syndrome. Most of the components in this combination are both food and medicine, with high safety, having good multi-target synergistic effects, and possessing the scientific basis for developing into functional tea drinks or metabolic regulation products.

[0025] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages: The traditional Chinese medicine preparation based on green tea of the present invention can effectively relieve hepatic gluconeogenesis caused by hyperglycemia, improve the disorder of hormones and signaling molecules caused by high sugar and high fat, and reduce hyperlipidemia caused by high-fat diet. The preparation method of the present invention is scientific and reasonable, can effectively retain the active ingredients of green tea, improve its bioavailability, has a good auxiliary effect on reducing blood lipid and blood sugar, is suitable for daily drinking by people with diabetes and abnormal blood lipid, and has broad application prospects. Description of the Drawings

[0026] Figure 1 It is a comparison chart of the in vitro blood sugar-lowering ability scores of different green teas. Different letters indicate significant differences between groups (P<0.05), and the same applies hereinafter.

[0027] Figure 2 It is a comparison chart of the in vitro blood sugar-lowering ability scores of different food-medicinal homologous substances.

[0028] Figure 3 It is a comparison chart of the in vitro blood sugar-lowering ability scores of different traditional Chinese medicine preparations of green tea.

[0029] Figure 4 It is a diagram of hepatic gluconeogenesis in rats. *, **, ***, **** indicate significant differences between groups (P<0.05), and the same applies hereinafter.

[0030] Figure 5 It is a diagram of the levels of hormones and signaling molecules in rat serum.

[0031] Figure 6 It is a diagram of the C-peptide level in rat serum.

[0032] Figure 7 It is a diagram of lipid metabolism in rat serum.

[0033] Figure 8 It is a diagram of glycated metabolites in rats.

[0034] Figure 9 It is a diagram of the OGTT curve in rats.

[0035] Figure 10 It is a diagram of the monitoring of rat body weight and fasting blood glucose. Detailed Embodiments

[0036] The technical solution of the present invention will be further described below in conjunction with the accompanying drawings.

[0037] Example 1 Screening of Formulation Ingredients

[0038] 1. Screening of Tea Varieties

[0039] Preparation method: (1) The tea leaves are pulverized at a high speed, with a rotation speed of 3500 - 4000 revolutions per minute, pulverized for 10 - 20 s each time, and pulverized 4 - 5 times in total. (2) The pulverized tea leaves are formulated at a material - to - water ratio of 1:100 (w / v) and extracted at 40°C for 80 min.

[0040] Tea varieties: Tieguanyin (S1), Anji Baicha (S2), Longjing (D1), Liuan Guapian (D2), Taihu Cuizhu (D3), Biluochun (D4), Lushan Yunwu (D5), and Lvyangchun (D6).

[0041] 2. Screening of Medicinal and Edible Homologous Plants

[0042] Preparation method: (1) The medicinal and edible homologous plants are removed of impurities, washed, dried, and then dried by hot air, with a drying temperature of 100 - 120°C and a drying time of 10 - 20 min. (2) They are pulverized at a high speed, with a rotation speed of 3000 - 4000 revolutions per minute, pulverized for 10 - 20 s each time, and pulverized 4 - 5 times in total. (3) The pulverized plants are formulated at a material - to - water ratio of 1:100 (w / v) and extracted by ultrasonic wave at 40°C for 80 min.

[0043] Medicinal and edible homologous plant varieties: Rubus chingii (S3), Morus alba leaf (S4), Lotus leaf (S5), Hawthorn (D7), Alpinia oxyphylla (D8), Corn silk (D9), Chrysanthemum (D10), Dendrobium officinale (D11), Honeysuckle (D12), Lycium barbarum (D13).

[0044] 3. Screening Test of Green Tea with In Vitro Inhibitory Activity against α - Glucosidase and Antioxidant Capacity

[0045] α - Glucosidase is a key enzyme in the brush border of small intestinal epithelial cells. Inhibiting the activity of α - glucosidase can delay the decomposition rate of carbohydrates into glucose, slow down glucose absorption, help reduce the pressure of insulin secretion, improve pancreatic islet function, reduce insulin resistance, and is an important strategy for controlling diabetes. In addition, oxidative stress is the core mechanism of diabetic complications (such as cardiovascular diseases, diabetic nephropathy, and retinopathy). Antioxidant effects can protect pancreatic islet β - cells, endothelial cells, and nerve cells, and reduce the risk of complications.

[0046] There is a synergistic effect between the various tea polyphenols rich in green tea and the active substances such as polyphenols and flavonoids rich in medicinal and edible homologous substances, which synergistically inhibits α-glucosidase, provides multi-target inhibition, delays the decomposition of disaccharides and the absorption of glucose. Medicinal and edible homologous substances achieve the inhibition of α-glucosidase and strong antioxidant effects through active ingredients (such as polyphenols, saponins, and vitamins, etc.).

[0047] Using the DPPH· free radical scavenging ability kit (purchased from Nanjing Jiancheng Bioengineering Institute, A53-1-1), Fe 2+ reduction ability kit (purchased from Nanjing Jiancheng Bioengineering Institute, A015-3-1) and ABTS + · free radical scavenging ability kit (purchased from Nanjing Jiancheng Bioengineering Institute, A015-2-1) to measure the in vitro inhibitory activity of α-glucosidase and antioxidant capacity, and normalize the measurement results (0-1 point) to obtain the hypoglycemic ability score. By comparing eight different regions of green tea (S1-S2, D1-D6), it was found that ( Figure 1 ), the hypoglycemic ability scores of green tea from high to low are: Tieguanyin, Anji Baicha, Longjing, Lu'an Melon Seed, Taihu Cuizhu, Biluochun, Lushan Yunwu, and Lvyangchun. Therefore, Tieguanyin and Anji Baicha with inhibitory activity against α-glucosidase and strong antioxidant capacity were selected for subsequent experiments. By comparing ten medicinal and edible homologous substances with potential for assisting in lowering blood sugar (S9-S11, D7-D13), it was found that ( Figure 2 ), the hypoglycemic ability scores of medicinal and edible homologous substances from high to low are: raspberry, mulberry leaf, lotus leaf, hawthorn, chrysanthemum, corn silk, semen myristicae, honeysuckle, dendrobium officinale, and wolfberry. Therefore, raspberry, lotus leaf, and mulberry leaf with inhibitory activity against α-glucosidase and strong antioxidant capacity were selected for subsequent experiments.

[0048] Example 2 Formula Ratio Screening

[0049] Preparation method: (1) Remove impurities, wash, dry the raw materials, and then perform hot air drying. The drying temperature is 100-120 °C, and the drying time is 10-20 min. (2) Grind the raw materials at a high speed, with a rotation speed of 3000-4000 revolutions per minute, each grinding for 10-20 s, and a total of 4-5 grindings. (3) Prepare the ground raw materials according to a material-to-water ratio of 1:100 (w / v), and perform ultrasonic-assisted extraction at 40 °C for 80 min.

[0050] Formula composition:

[0051] S6: 50 parts of Tieguanyin, 16 parts of mulberry leaf, 16 parts of raspberry, 16 parts of lotus leaf.

[0052] S7: 50 parts of Anji Baicha, 16 parts of mulberry leaf, 16 parts of raspberry, 16 parts of lotus leaf.

[0053] D14: 50 parts of Tieguanyin tea, 20 parts of mulberry leaves, 20 parts of raspberries, and 10 parts of lotus leaves.

[0054] D15: 40 parts of Tieguanyin tea, 20 parts of mulberry leaves, 20 parts of raspberries, and 20 parts of lotus leaves.

[0055] D16: 50 parts of Tieguanyin tea, 10 parts of mulberry leaves, 20 parts of raspberries, and 20 parts of lotus leaves.

[0056] D17: 50 parts of Tieguanyin tea, 20 parts of mulberry leaves, 10 parts of raspberries, and 20 parts of lotus leaves.

[0057] D18: 50 parts of Anji white tea, 20 parts of mulberry leaves, 20 parts of raspberries, and 10 parts of lotus leaves.

[0058] D19: 40 parts of Anji white tea, 20 parts of mulberry leaves, 20 parts of raspberries, and 20 parts of lotus leaves.

[0059] D20: 50 parts of Anji white tea, 10 parts of mulberry leaves, 20 parts of raspberries, and 20 parts of lotus leaves.

[0060] D21: 50 parts of Anji white tea, 20 parts of mulberry leaves, 10 parts of raspberries, and 20 parts of lotus leaves.

[0061] The screening test of green tea with in vitro inhibitory activity against α-glucosidase and antioxidant capacity was the same as that in Example 1. By comparing ten green tea beverages with potential for assisting in blood sugar reduction (S6 - S7, D14 - D21), their in vitro inhibitory activity against α-glucosidase and antioxidant capacity (DPPH· radical scavenging capacity, Fe 2+ reducing capacity, and ABTS + · radical scavenging capacity) were measured. The measurement results were normalized (0 - 1 point) to obtain the blood sugar reduction capacity score ( Figure 3 ). It was found that the in vitro inhibitory activity against α-glucosidase and strong antioxidant capacity of the S6 and S7 compositions were significantly higher than those of the comparative examples (D14 - D21). This indicates that green tea polyphenols and the composition of medicine and food homology can chelate transition metal ions (such as Fe2+, Cu2+), prevent the generation of hydroxyl radicals, and further reduce oxidative stress.

[0062] Animal experiment of green tea beverage with blood sugar reduction assistance in Example 3

[0063] (1) Preparation of gavage samples

[0064] Preparation method: (1) Remove impurities, wash, and dry lotus leaves, mulberry leaves, and raspberries, and then perform hot air drying at a drying temperature of 100 - 120°C for 10 - 20 minutes. (2) Mix the dried lotus leaves, mulberry leaves, raspberries, and green tea in proportion and perform high-speed crushing at a rotation speed of 3000 - 4000 revolutions per minute for 10 - 20 seconds each time, with a total of 4 - 5 crushing times. (3) Prepare the crushed composition according to the material-water ratio and perform ultrasonic-assisted extraction at 40°C for 80 minutes.

[0065] S6: 50 parts of Tieguanyin, 16 parts of mulberry leaves, 16 parts of raspberries, 16 parts of lotus leaves. The material-water ratio is 1:100 (w / v).

[0066] S7: 50 parts of Anji white tea, 16 parts of mulberry leaves, 16 parts of raspberries, 16 parts of lotus leaves. The material-water ratio is 1:100 (w / v).

[0067] D22: 50 parts of Tieguanyin, 16 parts of mulberry leaves, 16 parts of raspberries, 16 parts of lotus leaves. The material-water ratio is 1:200 (w / v). D23: 50 parts of Tieguanyin, 16 parts of mulberry leaves, 16 parts of raspberries, 16 parts of lotus leaves. The material-water ratio is 1:50 (w / v).

[0068] (2) Animal grouping and modeling

[0069] After adaptively raising 4-week-old male Waster rats (purchased from Jinan Pengyue Experimental Animal Breeding Co., Ltd., license number: SCXK(Lu)20220006, 120 ± 20 g) for 1 week, they were randomly divided into 7 groups with 8 rats in each group, namely the blank group, the model group, the positive control group, the Tieguanyin and medicine and food homology composition group, the Anji white tea and medicine and food homology composition group, the low-dose Tieguanyin and medicine and food homology composition group, and the high-dose Tieguanyin and medicine and food homology composition group. A high-fat diet combined with STZ was used to induce a high-fat and hyperglycemia rat model. Except for the normal group of animals, other rats were intraperitoneally injected with streptozotocin (STZ) (12 mg / mL) solution at a dose of 5 mL / kg according to body weight. All animals were injected within 30 minutes. The normal group was injected with an equal volume of citric acid-sodium citrate buffer solution. After 2 hours of injection, they resumed eating (normal rats were fed with ordinary feed, and model rats were fed with high-fat feed). Four days and seven days after modeling, the fasting blood glucose of the rats was measured with a blood glucose meter. Rats with a blood glucose value greater than 13 mmol / L were considered to have successfully modeled. After modeling was completed, a 4-week intervention was carried out. All samples were gavage-administered at a dose of 1.0 mL / 200 g·d. Water and feed were supplemented regularly every day. After gavage, the animals were observed, and their states were recorded in a timely manner. The bedding was changed four times a week. The specific grouping and treatment are shown in Table 1.

[0070] Table 1 Animal experiment grouping and treatment

[0071]

[0072] (3) Characterization of test results

[0073] ① Gluconeogenesis in rat liver

[0074] Glycogen, pyruvate and lactate in the liver play key roles in the occurrence and development of hyperglycemia. By participating in the key pathways of glucose metabolism, they jointly affect the regulation and homeostasis of blood glucose. After the intervention, after 24 hours of food and water deprivation, the rat liver was taken for liver homogenization, and then the supernatant of the liver homogenate was obtained by centrifugation. A glycogen kit (purchased from Nanjing Jiancheng Bioengineering Institute, A043-1-1), a pyruvate kit (purchased from Nanjing Jiancheng Bioengineering Institute, A081-1-1) and a lactate kit (purchased from Nanjing Jiancheng Bioengineering Institute, A019-2-1) were used to measure the contents of glycogen, pyruvate and lactate in the rat liver.

[0075] Results: To evaluate the effect of green tea beverage on liver glucose metabolism in hyperglycemic rats induced by high-fat diet combined with STZ, the contents of glycogen, pyruvate and lactate in the livers of rats in each group were measured. As Figure 4 can be seen, the contents of pyruvate, lactate and glycogen in the MC group were significantly lower than those in the NC group (P<0.05), indicating that the liver glucose metabolism of hyperglycemic rats was disordered, inhibiting glycolysis. The levels of pyruvate, lactate and glycogen in the PC group, S6 group and S7 group were significantly higher than those in the MC group (P<0.05). Among them, there was no significant difference in the levels of pyruvate and lactate between the S6 group and the S7 group and the NC group (P>0.05). The intervention effect of the S6 group was the best, and the pyruvate content recovered close to the normal level, which was better than that of the D22 and D23 groups. It is indicated that the intervention may partially restore the liver metabolic function by improving insulin sensitivity or regulating the glycolysis pathway.

[0076] ② Hormone and signaling molecule levels in rat serum

[0077] Insulin is one of the core pathological features of hyperglycemia, which leads to reduced sensitivity of peripheral tissues (such as the liver, muscle, and fat) to insulin. As a result, glucose uptake is decreased and gluconeogenesis is enhanced, thus causing an increase in blood glucose. Insulin-like growth factor-1 (IGF-1), as a structural and functional analogue of insulin, can relieve insulin resistance and improve blood glucose regulation by enhancing glucose uptake and metabolism in peripheral tissues. In addition, glucagon-like peptide-1 (GLP-1) is an incretin hormone secreted by intestinal L cells, which reduces postprandial blood glucose through mechanisms such as promoting insulin secretion, inhibiting glucagon release, and delaying gastric emptying. In summary, insulin resistance is the main driving factor of hyperglycemia, while IGF-1 and GLP-1 play important roles in alleviating hyperglycemia and preventing diabetic complications by regulating glucose metabolism through multiple targets and protecting pancreatic islet function, providing potential avenues for targeted therapy. After the intervention ended and the rats were starved and water-deprived for 24 h, rat serum was collected, and an insulin-like growth factor-1 ELISA kit (purchased from Shanghai Hualan Chemical Technology Co., Ltd.) and a glucagon-like peptide-1 ELISA kit (purchased from Shanghai Hualan Chemical Technology Co., Ltd.) were used to measure the contents of insulin, IGF-1, and GLP-1 in the serum of rats in each group.

[0078] Results: To evaluate the effects of green tea tea drinks on hormones and signaling molecules in the serum of hyperglycemic rats induced by a high-fat diet combined with STZ, the contents of insulin, insulin-like growth factor-1, and glucagon-like peptide-1 in the serum of rats in each group were measured. As Figure 5 can be seen, the insulin level in the rats of the MC group was significantly higher than that in the NC group (P < 0.05), up to 58.09 ± 4.06 mU / L, indicating that there is insulin resistance in the body under the hyperglycemic state, and the pancreas secretes excessive insulin to maintain blood glucose levels. In contrast, the PC group and the S6 and S7 groups showed varying degrees of downward trends in insulin levels. The intervention groups (especially S6) showed a significant downward trend compared with the MC group (P < 0.05). S6 and PC had a certain alleviating effect on the high insulin level caused by diabetes, while the effect of the S7 group was relatively weak. The insulin level in the D22 group was higher than that in the S6 group, and its regulatory effect on insulin secretion was weaker. The insulin level in the D23 group was significantly lower than that in the S6 and D22 groups, and it was more effective in reducing the insulin secretion level (P < 0.05), making it closer to the NC group (19.52 ± 3.76 mU / L). In addition, the levels of IGF-1 and GLP-1 in the MC group were significantly higher than those in the NC group (P < 0.05), up to 26.43 ± 1.69 ng / mL and 18.27 ± 1.52 pmol / L, respectively. There was no significant difference in the regulatory effects of IGF-1 and GLP-1 between the S6, S7 groups and the PC group (P > 0.05). These results help to further clarify the potential application value of the intervention groups in the treatment of diabetes.

[0079] ③ C-peptide level in rat serum

[0080] C-peptide is a by-product released when proinsulin is cleaved to generate insulin, and is secreted in equal amounts with insulin. Therefore, its level directly reflects the functional state of pancreatic islet β cells. After the intervention ended and the rats were starved and water-deprived for 24 h, rat serum was collected, and the C-peptide content in the serum of rats in each group was measured using a C-peptide ELISA kit (purchased from Shanghai Hualan Chemical Technology Co., Ltd.).

[0081] Results: To evaluate the effect of green tea beverage on C-peptide in the serum of hyperglycemic rats induced by high-fat diet combined with STZ, the C-peptide content in the serum of rats in each group was measured. As Figure 6 can be seen, the C-peptide level in the MC group was significantly higher than that in the NC group (P<0.05), up to 8.03±0.46 ng / mL. The C-peptide level in the S6 group had no significant difference from that in the PC group (P>0.05), which was 6.24±0.48 ng / mL. However, the C-peptide level in the S7 group was significantly lower than that in the S6 group (P<0.05), indicating better improvement of pancreatic islet β cell function. The C-peptide level in the S6 group was significantly lower than that in the D22 and D23 groups (P<0.05), which was 5.86±0.11 ng / mL.

[0082] ④ Lipid metabolism of rats

[0083] Total protein, total cholesterol and triglyceride are important metabolic markers reflecting hyperlipidemia levels. To evaluate the effect of green tea beverage on lipid metabolism of hyperglycemic rats induced by high-fat diet combined with STZ, after the intervention ended and the rats were starved and water-deprived for 24 h, the contents of total protein, total cholesterol and triglyceride in the glycated serum of rats in each group were measured using an automatic biochemical analyzer.

[0084] Results: As Figure 7 can be seen, the levels of total protein, total cholesterol and triglyceride in the MC group were significantly higher than those in the NC group (P<0.05), which were 90.53±10.91 g / L, 4.28±0.58 mmol and 4.95±1.09 mmol respectively. The levels of total protein, total cholesterol and triglyceride in the PC group, S6 group and S7 group were significantly lower than those in the MC group (P<0.05). Among them, the S6 group was significantly lower than the S7 group (P<0.05), indicating that S6 could more effectively control the cumulative damage of blood glucose.

[0085] ⑤ Glycated metabolites of rats

[0086] Glycated hemoglobin (HbA1c) and glycated serum protein (GSP) are important metabolic markers reflecting hyperglycemic levels. HbA1c is the product of the non-enzymatic reaction between glucose in the blood and hemoglobin in red blood cells, and its level reflects the average blood glucose concentration in the past 2 - 3 months. GSP is the product formed by the non-enzymatic glycosylation of glucose in serum with proteins (such as albumin). Its formation rate is faster, and the half-life is about 2 - 3 weeks. Therefore, it reflects the blood glucose level fluctuations in a shorter period. After the intervention ended and the rats were starved and dehydrated for 24 h, rat serum was collected, and the content of glycated serum protein in the serum of each group of rats and glycated hemoglobin in the plasma were measured using a glycated hemoglobin ELISA kit (purchased from Shanghai Hualan Chemical Technology Co., Ltd.) and a glycated serum protein ELISA kit (purchased from Shanghai Hualan Chemical Technology Co., Ltd.).

[0087] Results: To evaluate the effect of green tea beverage on glycated metabolites in hyperglycemic rats induced by high-fat diet combined with STZ, the contents of glycated hemoglobin and glycated serum protein in each group of rats were measured. As Figure 8 can be seen, the HbA1c level in the MC group was significantly higher than that in the NC group (P < 0.05) (463.69 ± 96.35 ng / mL). The HbA1c levels in the PC group, S6 group, and S7 group were all significantly lower than that in the MC group (P < 0.05), which were 340.35 ± 113.43 ng / mL, 221.55 ± 41.20 ng / mL, and 287.90 ± 27.61 ng / mL respectively. Among them, the S6 group was significantly lower than the S7 group (P < 0.05), indicating that S6 could more effectively control the cumulative damage of blood glucose. In addition, the GSP level in the MC group was significantly higher than that in the NC group (P < 0.05), up to 28.13 ± 5.31 mmol / L. The GSP levels in the S6 group and S7 group were both significantly lower than that in the MC group (P < 0.05), which were 16.93 ± 3.19 mmol / L and 12.79 ± 2.12 mmol / L respectively. Among them, the S7 group was significantly lower than the S6 group (P < 0.05), indicating that S7 could more effectively control the cumulative damage of glycated serum protein, being superior to D22 and D23.

[0088] ⑥ Oral glucose tolerance in rats

[0089] Oral glucose tolerance test (OGTT) is an important method to evaluate the body's response to glucose load, and its results directly reveal the pathogenesis of hyperglycemia and the relationship between insulin sensitivity and secretion function. On the day when the administration ended, the oral glucose tolerance test was conducted. After the animals were fasted for 6 h, all animals were intragastrically administered a glucose solution at 5 mL / kg of body weight, and the tail vein blood glucose concentration was measured at 0, 30, 60, 90, and 120 min after intragastric administration respectively.

[0090] Results: The OGTT curve was plotted according to the blood glucose concentration of rats at different times (Figure 9 ) The blood glucose curve of the rats in the NC group was stable, with a low peak and a rapid decline, indicating normal insulin secretion function. The body could quickly regulate the glucose level and maintain blood glucose homeostasis. The blood glucose level of the rats in the MC group was significantly higher than that of other groups, with a large peak and a slow recovery, indicating insulin resistance or impaired pancreatic islet function, which was a typical manifestation of hyperglycemia. The blood glucose recovery trend of the S6 group was better than that of the S7 group, indicating that the intervention effect of the S6 group was more significant, superior to D22 and D23.

[0091] ⑦ Tracking of rat body weight and fasting blood glucose

[0092] Results: To evaluate the effects of green tea beverages on the body weight and fasting blood glucose of hyperglycemic rats induced by high-fat diet combined with STZ, the body weight and fasting blood glucose were regularly recorded during the intervention period. The results were as Figure 10 shown. After 4 weeks of intervention, the body weight of the rats in the model group (MC) increased from 350.90 g to 371.55 g. After the intervention with green tea beverages, the body weight of the rats all increased by more than 410 g, showing no significant difference from the blank group (NC) (P>0.05), effectively alleviating the phenomenon of weight loss due to hyperglycemia. The blood glucose level of the rats in the NC group remained in a relatively low range, with an average value between 4 - 6 mmol / L, and the curve was stable without significant fluctuations (P>0.05), reflecting normal blood glucose regulation ability. The MC group was significantly higher than other groups (P<0.05), indicating the continuous presence of hyperglycemia, which might be related to insulin resistance or impaired pancreatic islet β cell function. After the intervention with S6 and S7, the fasting blood glucose level of the hyperglycemic rats decreased. Among them, the S6 group had the best effect in improving the hyperglycemic state, superior to D22 and D23.

Claims

1. A traditional Chinese medicine preparation based on green tea, characterized in that, The traditional Chinese medicine preparation is made from the following components by weight: 45-55 parts of green tea, 15-20 parts of lotus leaf, 15-20 parts of mulberry leaf, and 15-20 parts of raspberry.

2. The traditional Chinese medicine preparation based on green tea according to claim 1, characterized in that, The green tea includes Tieguanyin or Anji white tea.

3. The preparation method of the traditional Chinese medicine preparation based on green tea according to claim 1, characterized in that, It includes the following steps: (1) Remove impurities and wash the lotus leaf, mulberry leaf, and raspberry, and then perform hot air drying; (2) Mix the dried lotus leaf, mulberry leaf, raspberry and green tea in proportion and perform high-speed crushing, a total of 4-5 times; (3) Prepare the crushed composition according to the solid-liquid ratio and perform ultrasonic extraction.

4. The preparation method according to claim 3, characterized in that, In step (1), the temperature of the drying is 100-120 °C and the time is 10-20 min.

5. The preparation method according to claim 3, wherein In step (2), the rotation speed of the high-speed crushing is 3000-4000 revolutions per minute, and each crushing is 10-20 s.

6. The preparation method according to claim 3, characterized in that, In step (3), the solid-liquid ratio is 1:50-200; the ultrasonic conditions are extraction at 30-50 °C for 60-100 min.

7. Use of the traditional Chinese medicine preparation based on green tea according to claim 1 in preparing a green tea beverage for multi-target regulation of glycolipid metabolism, characterized in that, The multi-target regulation of glucose and lipid metabolism includes alleviating hepatic gluconeogenesis caused by hyperglycemia, improving the disorder of hormones and signaling molecules caused by high glucose and high fat, and reducing hyperlipidemia caused by high-fat diet.

8. The application according to claim 7, wherein The alleviation of hepatic gluconeogenesis caused by hyperglycemia includes regulating the levels of glycogen, pyruvate and lactate in the liver.

9. The application according to claim 7, characterized in that The improvement of the disorder of hormones and signaling molecules caused by high glucose and high fat includes regulating the levels of insulin, insulin-like growth factor-1, glucagon-like peptide-1 and C-peptide.

10. The application according to claim 7, wherein The reduction of hyperlipidemia caused by high-fat diet includes reducing the levels of total protein, total cholesterol and triglyceride in the serum.

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