Bacteriostatic curing hydrogel and preparation method and application thereof

Abstract

The application belongs to the technical field of maintenance hydrogel, and particularly relates to a bacteriostatic maintenance hydrogel and a preparation method and application thereof. The bacteriostatic maintenance hydrogel is prepared from a bacteriostatic sustained-release agent, a chitosan quaternary ammonium salt solution and a crosslinking agent. The bacteriostatic sustained-release agent comprises various bacteriostatic components such as active extract, carbomer, safflower seed oil and jojoba seed powder. Through the synergistic effect of the active extract, the carbomer, the safflower seed oil and the jojoba seed powder, the bacteriostatic maintenance hydrogel has the advantages of broad-spectrum bacteriostasis and high biocompatibility, can realize long-acting bacteriostasis, can promote wound healing and reduce drug resistance, and can be used for preparing a vaginal maintenance product or a wound dressing, and has a good application prospect in the medical field.

Description

Technical Field

[0001] This invention belongs to the field of maintenance hydrogel technology, specifically relating to an antibacterial maintenance hydrogel, its preparation method, and its application. Background Technology

[0002] The vaginal surface is composed of mucous membrane tissue. Due to the high estrogen levels in young women, the vagina has many folds, which can harbor bacteria. When the immune system is weakened, or if the vaginal environment is conducive to the growth of these pathogens, bacteria can multiply and cause vaginal inflammation. Therefore, the purpose of vaginal care is to regulate vaginal problems by maintaining the vagina's pH balance, cleanliness, and moisture.

[0003] Hydrogels are water-dispersed gels with a network cross-linked structure. They are water-soluble polymers with some hydrophobic groups and hydrophilic residues introduced into them. The hydrophilic residues combine with water molecules, connecting the water molecules inside the network, while the hydrophobic residues swell when they come into contact with water. Hydrogels are a type of polymer network system that is soft, can maintain a certain shape, and can absorb a large amount of water.

[0004] Hydrogels have wide applications in the medical field. They can serve as drug delivery systems, achieving sustained and stable therapeutic effects by controlling the rate and manner of drug release. Hydrogels also provide a moist environment, promoting wound healing, and are therefore commonly used in wound dressings and burn treatment. Hydrogel technology is also frequently used in vaginal care products.

[0005] Vaginal care hydrogels offer multiple benefits, helping women address issues like vaginal dryness, itching, and odor. Specifically, firstly, they provide long-lasting hydration and moisturization, effectively relieving vaginal dryness. They replenish vaginal moisture, increase the elasticity of the vaginal mucosa, keeping the vagina moist and reducing discomfort. Secondly, they possess antibacterial and anti-inflammatory properties. Containing natural antibacterial ingredients, they inhibit bacterial growth and prevent vaginal infections. They also alleviate itching and discomfort caused by vaginal inflammation. Furthermore, they regulate the vaginal pH balance, maintaining a healthy environment. They increase vaginal acidity, inhibiting the growth of harmful bacteria and protecting vaginal health. Finally, vaginal care hydrogels are very easy to use. Simply apply an appropriate amount to the vaginal opening and vaginal walls, then gently massage. They leave no residue inside the vagina and cause no discomfort.

[0006] However, existing vaginal care technologies have drawbacks such as over-cleaning, easy allergic reactions, drug resistance, and long-term dependence. Summary of the Invention

[0007] Based on the above technical background, the main objective of this invention is to provide an antibacterial and protective hydrogel, its preparation method, and its application, so as to overcome the shortcomings of the prior art.

[0008] To achieve the aforementioned objectives, the technical solution adopted by this invention includes:

[0009] The first aspect of this invention is to provide an antibacterial and protective hydrogel, wherein the antibacterial and protective hydrogel is prepared from an antibacterial sustained-release agent, a chitosan quaternary ammonium salt solution and a crosslinking agent;

[0010] The mass ratio of the antibacterial sustained-release agent, chitosan quaternary ammonium salt solution and crosslinking agent is (0.2-0.5):(0.7-1.3):1.11.

[0011] Preferably, the antibacterial sustained-release agent includes an active extract and carbomer;

[0012] The mass ratio of the active extract to carbomer is (4-7):(2-4).

[0013] More preferably, the active extract is selected from one or more of the following: Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and French sage extract.

[0014] More preferably, the active extract is obtained by mixing Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract and French sage extract in a mass ratio of (1-2):(1-2):(1-3):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2).

[0015] Preferably, the antibacterial sustained-release agent further includes safflower seed oil and jojoba seed powder.

[0016] More preferably, the mass ratio of the active extract, safflower seed oil and jojoba seed powder is (4-7):1:(2-4).

[0017] A second aspect of the present invention is to provide a method for preparing the antibacterial and protective hydrogel described in the first aspect of the present invention, the preparation method comprising the following steps:

[0018] Step 1: Add the antibacterial sustained-release agent to the solvent and stir well to obtain an antibacterial sustained-release solution;

[0019] Step 2: Add the crosslinking agent to the solvent and stir until homogeneous to obtain a crosslinking agent solution;

[0020] Step 3: Drop the antibacterial sustained-release solution into the cross-linking agent solution, let it stand at room temperature to react, then wash and dry to obtain antibacterial sustained-release particles;

[0021] Step 4: Mix the antibacterial sustained-release particles and the chitosan quaternary ammonium salt solution to obtain an antibacterial and protective hydrogel.

[0022] In step 3,

[0023] Preferably, the crosslinking agent is added dropwise to the crosslinking agent solution at a flow rate of 0.1 to 0.3 mL / min, and the reaction is allowed to proceed at room temperature for 3 to 6 hours.

[0024] In step 4,

[0025] Preferably, the antibacterial sustained-release granules and chitosan quaternary ammonium salt solution are mixed for 1 to 3 hours.

[0026] A third aspect of the present invention is to provide an application of the antibacterial and nourishing hydrogel described in the first aspect of the present invention in the preparation of vaginal care products or wound dressings.

[0027] The beneficial effects of this invention are as follows:

[0028] (1) The antibacterial and protective hydrogel of the present invention is mainly prepared by antibacterial sustained-release agent and chitosan quaternary ammonium salt solution. The antibacterial sustained-release agent includes a variety of antibacterial components such as active extract, carbomer, safflower seed oil and jojoba seed powder. Through the synergistic effect of active extract, carbomer, safflower seed oil and jojoba seed powder, the hydrogel is endowed with the advantages of broad-spectrum antibacterial and long-term antibacterial, while also having the advantages of high biocompatibility, long-term wetting and reduced drug resistance.

[0029] (2) The chitosan quaternary ammonium salt used in this invention has certain antibacterial activity. Due to the hydrophobic long chain and the hydrophobic affinity between it and the phospholipids on the bacterial cell membrane, the quaternary ions introduced into the quaternary ammonium salt molecular chain also act on the bacterial cell surface, thereby destroying the bacterial cell membrane, disrupting the normal physiological activities of bacteria, and destroying the internal physiological functions of bacteria, thus leading to bacterial death. Chitosan quaternary ammonium salt has antibacterial effects on a variety of bacteria, fungi and viruses, and can effectively inhibit their growth and reproduction. The active extract will act as a membrane disruptor, by destroying the membrane structure, causing intracellular potassium ion leakage, inhibiting cell respiration, stimulating cell autolysis, leading to loss of intracellular electron density substances, and changing cell morphology. The mixture of chitosan quaternary ammonium salt, active extract, carbomer, safflower seed oil and jojoba seed powder can produce a synergistic effect and has a broad-spectrum antibacterial effect on a variety of bacteria, fungi and viruses.

[0030] (3) Long-lasting antibacterial effect: The cross-linked structure of the antibacterial sustained-release particles can slow down the release rate of antibacterial substances such as active extracts, carbomer, safflower seed oil, jojoba seed powder, and chitosan quaternary ammonium salt, giving it a long-lasting antibacterial effect. The antibacterial sustained-release particles can achieve controlled release of active ingredients to different degrees by adjusting the degree of cross-linking and particle size, which can prolong the residence time of active ingredients in the body and improve their bioavailability. Controllable release rate of active ingredients: The antibacterial sustained-release particles can also control the release rate of active ingredients by adjusting the degree of cross-linking and particle size, achieving continuous and stable release of active ingredients and reducing the frequent use of antibacterial maintenance hydrogels. In addition, the antibacterial sustained-release particles encapsulate active extracts and other antibacterial components, forming a stable complex that protects the active ingredients from the influence of the external environment and extends their shelf life. This long-lasting antibacterial property can continuously inhibit the growth and reproduction of bacteria for a certain period of time.

[0031] (4) Chitosan is a natural product that does not involve chemical reagents in its preparation. It has good biocompatibility and biodegradability and will not cause significant toxic side effects or tissue damage. In addition, antibacterial sustained-release granules can release the active ingredients locally, reduce the distribution range of the active ingredients in the body, and reduce the toxic side effects of the active ingredients on non-target tissues.

[0032] (5) Chitosan quaternary ammonium salt can provide a good moisturizing environment and maintain a good humid environment. In addition, the viscosity and softness of the hydrogel described in this invention can also adapt well to the vaginal environment and reduce the risk of infection.

[0033] (6) Reducing drug resistance: Because hydrogels have multiple antibacterial mechanisms, including physical isolation, charge adsorption, and release of antibacterial substances, they can reduce the development of bacterial drug resistance. Natural active extracts can also reduce the development of drug resistance. Furthermore, in the embodiments of the present invention, the uncrosslinked chitosan quaternary ammonium salt, after release, forms a moist and adhesive microenvironment with the antibacterial sustained-release particles, which is beneficial to increase the in-situ sustained antibacterial time and reduce the body's clearance efficiency.

[0034] (7) The hydrogel of antibacterial sustained-release particles mixed with chitosan quaternary ammonium salt has advantages such as broad-spectrum antibacterial, long-lasting antibacterial, biocompatibility, promoting wound healing and reducing drug resistance. Moreover, the preparation method of this antibacterial maintenance hydrogel is simple and can be industrialized on a large scale. It is expected to be applied to vaginal care products or wound dressings. Detailed Implementation

[0035] The present invention will now be described in detail, and its features and advantages will become clearer and more apparent from these descriptions.

[0036] The first aspect of the present invention is to provide an antibacterial and protective hydrogel, wherein the antibacterial and protective hydrogel is prepared from an antibacterial sustained-release agent, a chitosan quaternary ammonium salt solution and a crosslinking agent.

[0037] The mass ratio of the antibacterial sustained-release agent, chitosan quaternary ammonium salt solution and crosslinking agent is (0.2-0.5):(0.7-1.3):1.11.

[0038] Preferably, the mass ratio of the antibacterial sustained-release agent, chitosan quaternary ammonium salt solution, and crosslinking agent is 0.3:1:1.11.

[0039] The antibacterial sustained-release agent includes active extracts, carbomer, safflower seed oil, and jojoba seed powder.

[0040] In the antibacterial sustained-release agent, the mass ratio of active extract, carbomer, safflower seed oil and jojoba seed powder is (4-7):(2-4):1:(2-4).

[0041] Preferably, the mass ratio of the active extract, carbomer, safflower seed oil, and jojoba seed powder is 6:3:1:3.

[0042] The active extract is selected from one or more of the following: Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and French sage extract.

[0043] Preferably, the active extract is a mixture of Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and Sage extract in a mass ratio of (1-2):(1-2):(1-3):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2).

[0044] Angelica sinensis extract has the effects of whitening, skin rejuvenation, regulating sebum secretion, anti-wrinkle and skin firming, anti-oxidation, anti-apoptosis, improving immunity, scavenging free radicals, promoting blood circulation and removing blood stasis, improving skin microcirculation and anti-aging.

[0045] Oligopeptide-3 can promote the growth and reproduction of skin tissue. By binding to specific receptors on the cell surface, it can regulate the division, reproduction, growth and differentiation of skin epithelial, endothelial and stromal cells, promote cell metabolism and enhance oxidation.

[0046] Acetyl hexapeptide-7 is a polypeptide composition with strong biological activity. It has excellent antioxidant, anti-wrinkle, collagen synthesis promotion and cell repair effects, and can significantly improve skin quality and delay aging.

[0047] Sophora flavescens has the effects of clearing heat and drying dampness, killing insects and relieving itching, inhibiting bacteria and reducing inflammation, anti-allergy, analgesia and beauty and skin care.

[0048] The main components of astragalus extract are flavonoids and polysaccharides. It has immunomodulatory effects, enhancing the body's immunity and resistance. Furthermore, astragalus extract also possesses antioxidant and anti-inflammatory properties, reducing the damage to the body caused by inflammatory responses and oxidative stress.

[0049] Tea tree oil extract can beautify the skin and remove acne. The tea tree essence it contains has anti-inflammatory and antibacterial effects, and its small molecular weight allows for good penetration when applied to the skin. This can repair damaged skin cells and achieve the effect of removing acne. It can also promote wound healing and kill bacteria.

[0050] Aloe vera extract has antibacterial, anti-inflammatory, beautifying, immune-boosting, skin-protecting, antibacterial, anti-inflammatory, analgesic, and wound-healing effects.

[0051] Camellia extract can combat aging, reduce wrinkles, improve dehydration, and also has anti-allergic and anti-inflammatory properties.

[0052] Deep-sea superoxide dismutase has antioxidant, free radical scavenging, anti-inflammatory, cell protection against damage, and organ function maintenance effects.

[0053] Oat extract and French sage extract are excellent antioxidants with firming, moisturizing, and mucosal care benefits; in addition, sage volatile oil has a strong, refreshing aroma and a calming and soothing effect.

[0054] The molar concentration of the chitosan quaternary ammonium salt solution is 0.1–0.3 M, preferably 0.2 M.

[0055] The crosslinking agent is selected from one or more of calcium chloride, zinc chloride, polyvinyl alcohol, and polyvinylpyrrolidone. Preferably, the crosslinking agent is calcium chloride or polyvinyl alcohol.

[0056] Chitosan quaternary ammonium salts offer several advantages: they can form a durable antibacterial protective film on surfaces, releasing antibacterial active substances for extended periods to continuously inhibit bacterial growth. Furthermore, chitosan quaternary ammonium salts exhibit low toxicity to humans and the environment, posing no significant harm to human health or the environment. Their antibacterial mechanism differs from traditional antibiotics, making them less likely to induce bacterial resistance. Finally, chitosan quaternary ammonium salts possess excellent adhesion, forming a uniform antibacterial protective layer on various surfaces, thus enhancing their antibacterial effect.

[0057] A second aspect of the present invention is to provide a method for preparing the antibacterial and protective hydrogel described in the first aspect of the present invention, the preparation method comprising the following steps:

[0058] Step 1: Add the antibacterial sustained-release agent to the solvent and stir well to obtain an antibacterial sustained-release solution;

[0059] Step 2: Add the crosslinking agent to the solvent and stir until homogeneous to obtain a crosslinking agent solution;

[0060] Step 3: Drop the antibacterial sustained-release solution into the cross-linking agent solution, let it stand at room temperature to react, then wash and dry to obtain antibacterial sustained-release particles;

[0061] Step 4: Mix the antibacterial sustained-release particles and the chitosan quaternary ammonium salt solution to obtain an antibacterial and protective hydrogel.

[0062] The steps described above are described in detail below.

[0063] In step 1, the solvent is selected from one or more of water, ethanol, and ethylene glycol. Preferably, the solvent is water.

[0064] There is no particular limit to the amount of solvent added, as long as it can completely dissolve the antibacterial slow-release agent.

[0065] The stirring speed is 80-120 rpm, and the stirring time is 10-30 min.

[0066] Preferably, the stirring speed is 100 rpm and the stirring time is 20 min.

[0067] In step 2, the crosslinking agent is added to water and stirred at a stirring speed of 100-200 rpm for 20-45 minutes.

[0068] Preferably, the crosslinking agent is added to water and stirred at 150 rpm for 30 minutes.

[0069] In step 3, the antibacterial sustained-release solution is added to the syringe pump and dripped into the crosslinking agent solution at a flow rate of 0.1 to 0.3 mL / min.

[0070] Preferably, the cross-linking agent solution is added dropwise at a flow rate of 0.2 mL / min. Experiments have shown that when the flow rate is within the above range, the resulting antibacterial sustained-release particles have a more uniform particle size.

[0071] The gelation reaction is achieved by allowing the mixture to stand at room temperature for 3 to 6 hours. Preferably, the gelation reaction is more complete by allowing the mixture to stand at room temperature for 5 hours, which can achieve full gelation.

[0072] After standing for reaction, the particles are gently washed with water to remove excess salts and other impurities from the surface, and then air-dried or dried at low temperature.

[0073] In step 4, the antibacterial sustained-release granules and chitosan quaternary ammonium salt solution are mixed for 1 to 3 hours, preferably for 2 hours.

[0074] A third aspect of the present invention is to provide an application of the antibacterial and nourishing hydrogel described in the first aspect of the present invention in the preparation of vaginal care products or wound dressings.

[0075] Example

[0076] The present invention is further illustrated below with specific examples. These embodiments are merely illustrative and not intended to limit the scope of the invention. All raw materials used in the embodiments of the present invention are commercially available.

[0077] Example 1

[0078] The raw materials were weighed according to a mass ratio of 0.3:1:1.11 for the antibacterial sustained-release agent, chitosan quaternary ammonium salt solution, and cross-linking agent. For the antibacterial sustained-release agent, the raw materials were weighed according to a mass ratio of 6:3:1:3 for the active extract, carbomer, safflower seed oil, and jojoba seed powder. For the active extract, the mass ratio of Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and Sage extract was 1:1:1:1:1:1:1:1:1:1:1:1. The molar concentration of the chitosan quaternary ammonium salt solution was 0.2M.

[0079] Add the weighed antibacterial sustained-release agent to water and stir at 100 rpm for 20 minutes to obtain an antibacterial sustained-release solution.

[0080] Calcium chloride was added to water and stirred at 150 rpm for 30 minutes to obtain a crosslinking agent solution, which served as a calcium ion source.

[0081] Add the antibacterial sustained-release solution to the injection pump and drip it into the crosslinking agent solution at a flow rate of 0.2 mL / min. Allow it to stand at room temperature for 5 hours to achieve the gelation reaction. Gently wash with water to remove excess salts and other impurities from the particle surface, and then air dry to obtain antibacterial sustained-release particles.

[0082] The antibacterial sustained-release particles and chitosan quaternary ammonium salt solution were mixed for 2 hours to obtain an antibacterial and protective hydrogel.

[0083] Example 2

[0084] The raw materials were weighed according to the mass ratio of antibacterial sustained-release agent, chitosan quaternary ammonium salt solution, and cross-linking agent of 0.2:0.7:1.11. In the antibacterial sustained-release agent, the raw materials were weighed according to the mass ratio of active extract, carbomer, safflower seed oil, and jojoba seed powder of 4:2:1:2. In the active extract, the mass ratio of Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and Sage extract was 1:2:1:1:2:2:1:1:1:1:1. The molar concentration of the chitosan quaternary ammonium salt solution was 0.2M.

[0085] Add the weighed antibacterial sustained-release agent to water and stir at 80 rpm for 30 minutes to obtain an antibacterial sustained-release solution.

[0086] Calcium chloride was added to water and stirred at 100 rpm for 45 minutes to obtain a crosslinking agent solution, which served as a calcium ion source.

[0087] Add the antibacterial sustained-release solution to the syringe pump and drip it into the crosslinking agent solution at a flow rate of 0.1 mL / min. Allow it to stand at room temperature for 3 hours to achieve the gelation reaction. Gently wash with water to remove excess salts and other impurities from the particle surface, and then air dry to obtain antibacterial sustained-release particles.

[0088] The antibacterial sustained-release particles and chitosan quaternary ammonium salt solution were mixed for 1 hour to obtain an antibacterial and protective hydrogel.

[0089] Example 3

[0090] The raw materials were weighed according to the mass ratio of antibacterial sustained-release agent, chitosan quaternary ammonium salt solution, and cross-linking agent of 0.5:1.3:1.11. In the antibacterial sustained-release agent, the raw materials were weighed according to the mass ratio of active extract, carbomer, safflower seed oil, and jojoba seed powder of 7:4:1:4. In the active extract, the mass ratio of Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and Sage extract was 2:2:3:2:2:2:2:2:2:2:2:2. The molar concentration of the chitosan quaternary ammonium salt solution was 0.2M.

[0091] Add the weighed antibacterial sustained-release agent to water and stir at 120 rpm for 10 minutes to obtain an antibacterial sustained-release solution.

[0092] Calcium chloride was added to water and stirred at 200 rpm for 20 minutes to obtain a crosslinking agent solution, which served as a calcium ion source.

[0093] Add the antibacterial sustained-release solution to the syringe pump and drip it into the crosslinking agent solution at a flow rate of 0.3 mL / min. Allow it to stand at room temperature for 6 hours to achieve the gelation reaction. Gently wash with water to remove excess salts and other impurities from the particle surface, and then air dry to obtain antibacterial sustained-release particles.

[0094] Antibacterial sustained-release particles and chitosan quaternary ammonium salt solution were mixed for 3 hours to obtain antibacterial and protective hydrogel.

[0095] The present invention has been described in detail above with reference to specific embodiments and exemplary examples; however, these descriptions should not be construed as limiting the present invention. Those skilled in the art will understand that various equivalent substitutions, modifications, or improvements can be made to the technical solutions and embodiments of the present invention without departing from the spirit and scope of the invention, and all such modifications and improvements fall within the scope of the present invention. The scope of protection of the present invention is defined by the appended claims.

Claims

1. An antibacterial and protective hydrogel, characterized in that, The antibacterial and protective hydrogel is prepared from an antibacterial sustained-release agent, a chitosan quaternary ammonium salt solution and a crosslinking agent; The mass ratio of the antibacterial sustained-release agent, chitosan quaternary ammonium salt solution, and crosslinking agent is (0.2-0.5):(0.7-1.3):1.11; The antibacterial sustained-release agent includes an active extract and carbomer; The mass ratio of the active extract to carbomer is (4-7):(2-4); The active extract is selected from one or more of the following: Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract, and French sage extract.

2. The antibacterial and protective hydrogel according to claim 1, characterized in that, The active extract is obtained by mixing Angelica sinensis extract, oligopeptide-3, acetyl hexapeptide-7, Sophora flavescens extract, Astragalus membranaceus extract, tea tree oil extract, aloe vera extract, camellia extract, deep-sea superoxide dismutase, oat extract and French sage extract in a mass ratio of (1-2):(1-2):(1-3):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2):(1-2).

3. The antibacterial and protective hydrogel according to claim 1, characterized in that, The antibacterial sustained-release agent also includes safflower seed oil and jojoba seed powder.

4. The antibacterial and protective hydrogel according to claim 3, characterized in that, The mass ratio of the active extract, safflower seed oil and jojoba seed powder is (4-7):1:(2-4).

5. A method for preparing the antibacterial and protective hydrogel according to any one of claims 1 to 4, characterized in that, The preparation method includes the following steps: Step 1: Add the antibacterial sustained-release agent to the solvent and stir well to obtain an antibacterial sustained-release solution; Step 2: Add the crosslinking agent to the solvent and stir until homogeneous to obtain a crosslinking agent solution; Step 3: Drop the antibacterial sustained-release solution into the cross-linking agent solution, let it stand at room temperature to react, then wash and dry to obtain antibacterial sustained-release particles; Step 4: Mix the antibacterial sustained-release particles and the chitosan quaternary ammonium salt solution to obtain an antibacterial and protective hydrogel.

6. The preparation method according to claim 5, characterized in that, In step 3, Add the solution dropwise at a flow rate of 0.1–0.3 mL / min to the crosslinking agent solution and allow it to stand at room temperature for 3–6 hours to react.

7. The preparation method according to claim 5, characterized in that, In step 4, Mix the antibacterial sustained-release granules and chitosan quaternary ammonium salt solution for 1–3 hours.

8. The use of the antibacterial and protective hydrogel according to any one of claims 1 to 4 in the preparation of vaginal care products or wound dressings.