Medical examination microorganism culture device and culture method

Abstract

The application discloses a medical examination microorganism culture device and a culture method, relates to the technical field of microorganism culture, and comprises a cabinet body, a thermostat is fixedly connected in the cabinet body, a culture box is slidably connected in the thermostat, a gas supplement mechanism is connected between the culture box and the thermostat, the gas supplement mechanism is used for supplementing gas into the culture box, an adjusting mechanism is arranged in the gas supplement mechanism and is used for adjusting the amount of gas supplemented into the culture box by the gas supplement mechanism, an exhaust mechanism is arranged on the side, away from the gas supplement mechanism, of the culture box and is used for intermittently adjusting the gas density in the culture box, when the culture box slides, the culture box can adjust the gas intake amount of the gas supplement mechanism through the adjusting mechanism, the exhaust mechanism can be intermittently opened and closed, the gas pressure in the culture box is increased, and the gas in the culture box is exhausted through the exhaust mechanism, so that the microorganism culture can be assisted.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbial culture, in particular to a medical examination microbial culture device and culture method. BACKGROUND

[0002] Microbial culture is to inoculate samples into one or more culture bottles or culture dishes to find and identify bacteria or other cultivable microorganisms, and it has important clinical significance for the diagnosis, treatment and prognosis of infectious diseases by detecting microorganisms in the blood of patients. In medical examination, it is often necessary to culture microorganisms or corresponding bacteria for observation, so as to carry out immune clinical treatment and medical care procedures. Therefore, the effective culture of various bacterial microorganisms is very important in the clinical laboratory.

[0003] At present, when culturing microorganisms, the culture bottles or culture dishes containing microorganisms are usually placed in a culture box for shaking or static culture at a specific temperature and humidity, so that the microorganisms can better absorb nutrient solution. Since there are four growth stages, i.e. adjustment period, logarithmic phase, stationary phase and decline phase, when culturing microorganisms that need shaking assistance, pure gas needs to be supplemented to meet the gas conditions of different growth periods of microorganisms. Since microorganisms have different growth stages, the amount of gas required in different growth stages is also different. The current equipment can only shake to assist the culture of microorganisms, and cannot adjust the amount of gas required according to the state of different growth periods of microorganisms. After a large amount of pure gas is injected, carbon dioxide and other gases can be produced in the process of rapid growth of microorganisms, so that the gas pressure in the culture device gradually increases. The mixed gas produced by microorganisms and pure gas is not conducive to the growth of microorganisms. Moreover, the culture device is directly connected to the outside for exhaust, which is not conducive to the better growth of microorganisms assisted by pure gas.

[0004] Therefore, the present application provides a medical examination microbial culture device and culture method to solve the above problems. SUMMARY

[0005] The main purpose of the present application is to provide a medical examination microbial culture device and culture method, which can effectively solve the technical problems in the background art.

[0006] In order to achieve the above object, the technical scheme adopted by the present application is as follows: a medical examination microorganism culture device, comprising a cabinet, a thermostat box is fixedly connected in the cabinet, a culture box is slidably connected in the thermostat box, a gas supplement mechanism is connected between the culture box and the thermostat box, the gas supplement mechanism is used for supplementing gas into the culture box, an adjusting mechanism is arranged in the gas supplement mechanism and is used for adjusting the amount of gas supplemented into the culture box by the gas supplement mechanism, and an exhaust mechanism is arranged on the side of the culture box away from the gas supplement mechanism and is used for intermittently adjusting the gas density in the culture box.

[0007] When the culture box slides, the culture box can adjust the air intake amount of the gas supplement mechanism through the adjusting mechanism, the exhaust mechanism can be intermittently opened and closed, the gas pressure in the culture box is increased, and the gas in the culture box is discharged through the exhaust mechanism.

[0008] Preferably, the gas supplement mechanism comprises a connecting block fixedly connected with the thermostat box, a gas supplement channel is formed in the connecting block, one end of the gas supplement channel close to the culture box is communicated with the culture box through a connecting pipe, and the other end of the gas supplement channel away from the culture box is fixedly connected with a gas supplement pipe, and the free end of the gas supplement pipe extends out of the thermostat box and the cabinet in sequence.

[0009] Preferably, the adjusting mechanism comprises a containing groove formed in the connecting block, a rotating ring is rotatably connected in the containing groove, an adjusting plate is fixedly connected to one end of the rotating ring close to the connecting pipe, a plurality of arc-shaped grooves are formed in the adjusting plate and are distributed in a circumferential direction at intervals, a plurality of baffle plates corresponding to the arc-shaped grooves are slidably connected in the connecting block, the plurality of baffle plates can block the gas supplement channel, one end of each baffle plate away from the gas supplement channel is fixedly connected with a plug rod, and the free end of each plug rod is inserted into the corresponding arc-shaped groove, when the adjusting plate rotates, the plurality of plug rods can slide in the direction of the gas supplement channel along the arc-shaped grooves.

[0010] Preferably, an inclined guide groove is formed in the circumferential side of the rotating ring, a vertically extending guide rod is slidably connected to the connecting block, a jacking rod is fixedly connected to one side of the guide rod close to the rotating ring, the jacking rod is slidably connected with the guide groove, a pressing block is arranged on one side of the culture box close to the connecting block, the side of the pressing block close to the connecting block is a wedge surface, one end of the guide rod close to the culture box extends out of the connecting block and can contact the pressing block, an electric telescopic rod is connected in the culture box, the output end of the electric telescopic rod is fixedly connected with the pressing block, and the guide rod and the connecting block are connected through a second elastic member.

[0011] Preferably, the exhaust mechanism comprises an exhaust groove transversely arranged on the incubator, a blocking plate is slidably connected in the exhaust groove, a plurality of circumferentially spaced gas outlet grooves are arranged on the blocking plate, a plurality of blocking blocks corresponding to the gas outlet grooves are fixedly connected in the exhaust groove, each blocking block can extend into the corresponding gas outlet groove, and a positioning ring is fixedly connected in the exhaust groove and connected with the blocking plate through a first elastic member.

[0012] Preferably, the incubator is fixedly connected with an auxiliary block on one side close to the exhaust groove, an air outlet groove is arranged on the auxiliary block and communicates with the gas outlet groove, a slide is arranged on the auxiliary block and communicates with the air outlet groove, an intermittent plate is slidably connected in the slide and blocks the air outlet groove, a gas guide groove is arranged on the intermittent plate and can be located in the air outlet groove, and the end of the blocking plate away from the incubator can be inserted into the gas guide groove.

[0013] Preferably, a piston cavity communicating with the slide is arranged in the auxiliary block, a piston rod is slidably connected in the piston cavity, and the end of the piston rod away from the incubator can be in contact with the inner wall of the incubator, so that the piston rod can drive the intermittent plate to slide when the piston rod slides.

[0014] Preferably, a supporting block is fixedly connected in the incubator and located below the incubator, a driving motor is mounted on the supporting block, a limiting ring is fixedly connected on one side of the incubator close to the driving motor, a disc is coaxially fixedly connected to the output end of the driving motor, a push rod is eccentrically fixedly connected to the disc and extends into the limiting ring, a mass sensor is connected to the incubator, and the mass sensor is electrically connected with the driving motor and the electric telescopic rod.

[0015] Preferably, a cabinet door capable of blocking the incubator is hingedly connected to the cabinet body, a transparent plate capable of blocking the opening of the incubator is hingedly connected to the opening of the incubator, and a clamping groove is arranged on the inner wall of the incubator.

[0016] A medical examination microorganism culture method comprises the following steps:

[0017] S1: first, place a culture dish or a culture bottle containing microorganisms in the incubator, and then adjust the incubator to provide a stable growth environment;

[0018] S2: then, start the driving motor, and the driving motor drives the microorganisms in the incubator to shake, thereby improving the efficiency of microorganism growth;

[0019] S3: then, through the shaking of the incubator, the air supplement mechanism supplements air into the incubator, and the adjusting mechanism can adjust the air volume according to different growth stages of microorganisms.

[0020] S4: Finally, through the shaking of the incubator, the exhaust mechanism can be intermittently opened, thereby ensuring the stability of the gas inside the incubator, benefiting the growth of microorganisms, and after the incubation is completed, the culture dish or culture bottle can be taken out.

[0021] The technical effects and advantages of the present application are as follows:

[0022] 1、The present application adjusts the position of the pressing block through the mass sensor and the electric telescopic rod, so that the area of the air supplement channel blocked by the plurality of baffles can be adjusted through the pressing block, guide rod and rotating ring, thereby automatically adjusting the air intake of the air supplement channel in different growth periods of microorganisms, reasonably controlling the air intake, facilitating the gas demand of microorganisms in different growth periods, and facilitating the better growth of microorganisms.

[0023] 2、The present application can intermittently extrude the piston rod through the shaking of the incubator, so that the exhaust groove and the air release groove are intermittently connected through the air guide groove, so that the gas in the incubator can stably react with microorganisms, and when the gas pressure in the incubator gradually increases, the gas pressure overcomes the elastic force of the first elastic member, so that the blocking block is separated from the air release groove, facilitating the discharge of the gas in the incubator, providing a stable gas environment for the growth of microorganisms, and according to the different frequency of the shaking of the incubator, the gas discharge interval in the incubator will also increase, so that the pure gas entering the incubator stably contacts and reacts with microorganisms, which can effectively improve the effect of microorganism culture. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 It is a whole schematic diagram of the present application;

[0025] Figure 2 It is a sectional view of the present application;

[0026] Figure 3 It is a structure schematic diagram of the incubator in the present application;

[0027] Figure 4 It is a structure schematic diagram of the supporting block in the present application;

[0028] Figure 5 It is a sectional view of the incubator and the supporting block in the present application;

[0029] Figure 6 It is an enlarged view of A in the present application; Figure 5

[0030] Figure 7 It is a sectional view of the structure of the auxiliary block in the present application;

[0031] Figure 8 ​It is a schematic view of the display structure of the exhaust mechanism in the application;

[0032] Figure 9 It is a schematic view of the overall structure of the air supplement mechanism in the application;

[0033] Figure 10 It is a schematic view of the sectional structure of the air supplement mechanism in the application;

[0034] Figure 11 It is a schematic view of the structure of the adjusting mechanism in the application.

[0035] In the figure: 1, cabinet body; 2, constant temperature box; 3, incubator;

[0036] 4, air supplement mechanism; 401, connecting block; 402, air supplement channel; 403, connecting pipe; 404, air supplement pipe;

[0037] 5, adjusting mechanism; 501, containing groove; 502, rotating ring; 503, adjusting plate; 504, arc-shaped groove; 505, baffle; 506, insertion rod; 507, guide groove; 508, guide rod; 509, jacking rod; 510, pressing block; 511, electric telescopic rod; 512, second elastic member;

[0038] 6, exhaust mechanism; 601, exhaust groove; 602, plugging plate; 603, air outlet groove; 604, plugging block; 605, positioning ring; 606, first elastic member; 607, auxiliary block; 608, air release groove; 609, slide; 610, intermittent plate; 611, air guide groove; 612, piston cavity; 613, piston rod;

[0039] 7, supporting block; 8, driving motor; 9, limiting ring; 10, disc; 11, push rod; 12, mass sensor; 13, cabinet door; 14, transparent plate; 15, clamping groove. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the application will be described clearly and completely below with reference to the drawings in the embodiments of the application. Obviously, the described embodiments are only part of the embodiments of the application, rather than all the embodiments of the application. Based on the embodiments in the application, all other embodiments obtained by those skilled in the art without creative work fall within the protection scope of the application.

[0041] Embodiment 1

[0042] When the microorganism is cultured for examination, the culture device is usually used to provide a suitable environment for the growth of the microorganism, and the shaking method is also used to assist the culture of the microorganism. However, in the process of culture, the microorganism has different growth stages, and the amount of gas required in different growth stages is different. The current device can only shake to assist the culture of the microorganism, and cannot adjust the amount of gas required according to the state of the different growth cycle of the microorganism. Therefore, the embodiment is based on the above problems.

[0043] As shown in Figure 1 Figure 11 The embodiment provides a medical examination microorganism culture device, which comprises a cabinet body 1, a thermostat 2 fixedly connected in the cabinet body 1, a culture box 3 slidably connected in the thermostat 2, a gas supplement mechanism 4 connected between the culture box 3 and the thermostat 2, the gas supplement mechanism 4 being used for supplementing gas into the culture box 3, an adjusting mechanism 5 arranged in the gas supplement mechanism 4 and used for adjusting the amount of gas supplemented into the culture box 3 by the gas supplement mechanism 4, and an exhaust mechanism 6 arranged on a side of the culture box 3 away from the gas supplement mechanism 4 and used for intermittently adjusting the gas density in the culture box 3.

[0044] When the culture box 3 slides, the culture box 3 can adjust the gas inlet amount of the gas supplement mechanism 4 through the adjusting mechanism 5, and the exhaust mechanism 6 can be intermittently opened and closed, so that the gas pressure in the culture box 3 is increased, and the gas in the culture box 3 is discharged through the exhaust mechanism 6.

[0045] The gas supplement mechanism 4 comprises a connecting block 401 fixedly connected with the thermostat 2, a gas supplement channel 402 is formed in the connecting block 401, one end of the gas supplement channel 402 close to the culture box 3 is communicated with the culture box 3 through a connecting pipe 403, and the other end of the gas supplement channel 402 away from the culture box 3 is fixedly connected with a gas supplement pipe 404, and the free end of the gas supplement pipe 404 extends out of the thermostat 2 and the cabinet body 1 in sequence.

[0046] The adjusting mechanism 5 comprises a containing groove 501 formed in the connecting block 401, a rotating ring 502 is rotatably connected in the containing groove 501, the rotating ring 502 is fixedly connected with an adjusting plate 503 at one end close to the connecting pipe 403, a plurality of arc-shaped grooves 504 are formed in the adjusting plate 503 and are distributed in a circumferential direction at intervals, a plurality of baffle plates 505 corresponding to the arc-shaped grooves 504 are slidably connected in the connecting block 401, the plurality of baffle plates 505 can block the gas supplement channel 402, one end of each baffle plate 505 away from the gas supplement channel 402 is fixedly connected with a plug rod 506, and the free end of each plug rod 506 is inserted into the corresponding arc-shaped groove 504, when the adjusting plate 503 rotates, the plurality of plug rods 506 can slide in the direction of the gas supplement channel 402 along the arc-shaped grooves 504.

[0047] ​The circumferential side of the rotating ring 502 is provided with an inclined guide groove 507, the connecting block 401 is slidably connected with a vertically extending guide rod 508, the side close to the rotating ring 502 of the guide rod 508 is fixedly connected with a jacking rod 509, the jacking rod 509 is slidably connected with the guide groove 507, the side close to the connecting block 401 of the incubator 3 is provided with a pressing block 510, the side close to the connecting block 401 of the pressing block 510 is a wedge surface, the end close to the incubator 3 of the guide rod 508 extends out of the connecting block 401 and can contact the pressing block 510, the incubator 3 is connected with an electric telescopic rod 511, the output end of the electric telescopic rod 511 is fixedly connected with the pressing block 510, and the guide rod 508 and the connecting block 401 are connected through a second elastic element 512.

[0048] The incubator 3 is fixedly connected with a supporting block 7 located at the lower side of the incubator 3, the supporting block 7 is installed with a driving motor 8, the side close to the driving motor 8 of the incubator 3 is fixedly connected with a limiting ring 9, the output end of the driving motor 8 is coaxially fixedly connected with a disc 10, the disc 10 is eccentrically fixedly connected with a push rod 11, the push rod 11 extends into the limiting ring 9, the incubator 3 is connected with a mass sensor 12, the mass sensor 12 is electrically connected with the driving motor 8 and the electric telescopic rod 511, the cabinet 1 is hingedly connected with a cabinet door 13 capable of blocking the incubator 2, the opening of the incubator 3 is hingedly connected with a transparent plate 14 capable of blocking the opening, and the inner wall of the incubator 3 is provided with a clamping groove 15.

[0049] In use, first, the cabinet door 13 in the cabinet 1 is opened, and the transparent plate 14 blocking the incubator 3 is opened, the culture dish or culture bottle containing microorganisms is placed in the clamping groove 15 in the incubator 3, so as to avoid the sliding of the culture dish or culture bottle caused by the shaking of the incubator 3, after the placement, the transparent plate 14 blocks the opening of the incubator 3 again, so as to ensure the airtightness of the inside of the incubator 3, then the cabinet door 13 is closed, so as to provide a stable and reliable growth environment, then the driving motor 8 is started, the driving motor 8 drives the push rod 11 to rotate through the disc 10, when the eccentric points of the disc 10 and the push rod 11 rotate from the most front side to the most right side, at this time, the disc 10 drives the incubator 3 to slide along the supporting block 7 to the direction close to the connecting block 401 through the push rod 11 and the limiting ring 9, so that the incubator 3 drives the culture liquid in the culture dish or culture bottle to shake, so as to facilitate the microorganisms to fully contact and react with the culture liquid.

[0050] When the incubator 3 drives the pressing block 510 to move towards the connecting block 401, and since the side of the pressing block 510 close to the connecting block 401 is an inclined surface, the wedge surface on the pressing block 510 can drive the guide rod 508 to slide along the connecting block 401 after the pressing block 510 contacts the guide rod 508, so that the guide rod 508 drives the top rod 509 to move, the second elastic member 512 is compressed, and the guide groove 507 is arranged in an inclined state, the guide rod 508 drives the top rod 509 to slide along the guide groove 507, so that the top rod 509 drives the rotating ring 502 to rotate along the accommodating groove 501 through the guide groove 507, so that the rotating ring 502 drives the adjusting plate 503 to rotate synchronously, since the distance between the arc-shaped groove 504 and the center of the adjusting plate 503 gradually increases in the clockwise direction, the adjusting plate 503 drives the plurality of insertion rods 506 to slide radially outward through the plurality of arc-shaped grooves 504, the plurality of insertion rods 506 drive the plurality of baffles 505 to slide radially outward along the air supplementing channel 402, so that the plurality of baffles 505 gradually release the air supplementing channel 402 from the plugging state, and the air supplementing pipe 404 is in communication with the pure gas container outside, so that the pure gas flows into the incubator 3 through the air supplementing pipe 404, the air supplementing channel 402 and the connecting pipe 403, so that the pure gas can also provide a gas environment for the growth of microorganisms.

[0051] When the eccentric point of the disc 10 and the push rod 11 rotates from the rightmost side to the leftmost side, at this time the disc 10 drives the incubator 3 to slide away from the connecting block 401 along the supporting block 7 through the push rod 11 and the limiting ring 9, so that the pressing block 510 no longer presses the guide rod 508, under the action of the second elastic member 512, the second elastic member 512 drives the guide rod 508 to gradually slide to the initial position, so that the guide rod 508 drives the top rod 509 to slide along the guide groove 507, so that the top rod 509 drives the rotating ring 502 to rotate along the accommodating groove 501 through the guide groove 507, so that the rotating ring 502 drives the adjusting plate 503 to rotate synchronously, so that the adjusting plate 503 drives the plurality of insertion rods 506 to slide radially inward through the plurality of arc-shaped grooves 504, the plurality of insertion rods 506 drive the plurality of baffles 505 to slide radially inward along the air supplementing channel 402, so that the plurality of baffles 505 again plugging the air supplementing channel 402, to avoid excessive pure gas entering the incubator 3.

[0052] When the mass sensor 12 detects that the mass on the incubator 3 changes rapidly, at this time the microorganism enters the rapid growth period, so that the mass sensor 12 transmits a signal to the driving motor 8 and the electric telescopic rod 511, so that the rotating speed of the driving motor 8 is reduced, the oscillation frequency of the incubator 3 is reduced, and the electric telescopic rod 511 is retracted to drive the pressing block 510 to move. Since the vertical distance between the pressing block 510 and the guide rod 508 is reduced, the distance that the pressing block 510 pushes the guide rod 508 to slide along the connecting block 401 is increased, so that the guide rod 508 drives the rotating ring 502 to rotate through the top rod 509 and the guide groove 507, and the distance that the plurality of baffles 505 slide radially outward is increased, thereby reducing the plugging area of the air supplement channel 402 and increasing the air intake of the air supplement channel 402, increasing the amount of pure gas supplemented into the incubator 3, so that the pure gas can meet the gas conditions for the rapid growth of the microorganism, and the microorganism grows more comprehensively.

[0053] When the microorganism growth period enters the process of the stable period and the decline period, at this time the mass of the microorganism changes no longer rapidly, so that the mass sensor 12 transmits a detection signal to the electric telescopic rod 511, so that the electric telescopic rod 511 drives the pressing block 510 to gradually slide towards the incubator 3, so that the pressing block 510 gradually reduces the travel of the guide rod 508, thereby adjusting the area of the plurality of baffles 505 plugging the air supplement channel 402, so that the pure gas entering the incubator 3 meets the gas environment for the growth of the microorganism, thereby further improving the effect of microorganism culture.

[0054] In summary, through the mass change of the microorganism in different growth periods, the mass sensor 12 adjusts the position of the pressing block 510 through the electric telescopic rod 511, so that the area of the plurality of baffles 505 plugging the air supplement channel 402 is adjusted through the pressing block 510, the guide rod 508 and the rotating ring 502, so that the air intake of the air supplement channel 402 can be automatically adjusted in different growth periods of the microorganism, the air intake is reasonably controlled, the gas demand of the microorganism in different growth periods is met, and the microorganism grows better.

[0055] Embodiment 2

[0056] When a large amount of pure gas is injected into the incubator 3, and carbon dioxide and other gases are generated during the rapid growth of the microorganism, the gas pressure in the incubator 3 gradually increases, and the mixture of the gas generated by the microorganism itself and the pure gas is not conducive to the growth of the microorganism. When the incubator 3 is communicated with the thermostat 2 through the hole, the gas in the incubator 3 can be directly discharged, but the contact reaction time of the pure gas with the microorganism is reduced, which is not conducive to the better growth of the microorganism, and therefore the above embodiment is further improved.

[0057] like Figure 5 - Figure 11 As shown, the exhaust mechanism 6 includes an exhaust channel 601 that is horizontally connected to the incubator 3. A sealing plate 602 is slidably connected inside the exhaust channel 601. The sealing plate 602 has multiple circumferentially spaced air outlet slots 603. Multiple sealing blocks 604 corresponding to the air outlet slots 603 are fixedly connected inside the exhaust channel 601. Each sealing block 604 can extend into the corresponding air outlet slot 603. A positioning ring 605 is fixedly connected inside the exhaust channel 601. The positioning ring 605 is connected to the sealing plate 602 through a first elastic member 606. An auxiliary block 607 is fixedly connected to the side of the incubator 3 near the exhaust channel 601. The auxiliary block 607 has openings corresponding to the air outlet slots 603. The air venting channel 608 is connected to the air venting channel 603. The auxiliary block 607 has a slide 609 that is connected to the air venting channel 608. An intermittent plate 610 that blocks the air venting channel 608 is slidably connected in the slide 609. An air guide channel 611 that can be located in the air venting channel 608 is opened on the intermittent plate 610. The end of the blocking plate 602 away from the incubator 3 can be inserted into the air guide channel 611. A piston chamber 612 that is connected to the slide 609 is opened in the auxiliary block 607. A piston rod 613 is slidably connected in the piston chamber 612. The end of the piston rod 613 away from the incubator 3 can contact the inner wall of the constant temperature chamber 2. When the piston rod 613 slides, it can drive the intermittent plate 610 to slide.

[0058] In use, when the eccentric point of the disc 10 and the push rod 11 rotates from the foremost position to the leftmost position, the incubator 3 causes the auxiliary block 607 to move away from the connecting block 401, causing the side wall of the constant temperature chamber 2 to press against the piston rod 613, causing the piston rod 613 to slide along the piston chamber 612 towards the incubator 3, thus compressing the gas in the piston chamber 612. Under the action of the gas, the intermittent plate 610 slides along the slide rail 609, causing the intermittent plate 610 to drive the gas guide groove 611 to slide into the gas release groove 608. At this time, because the gas pressure in the incubator 3 is slightly higher, the gas in the incubator 3 causes the sealing plate 602 to slide along the exhaust groove 601, and the first wave... When the elastic element 606 is compressed, the end of the sealing plate 602 away from the incubator 3 is inserted into the air guide groove 611. The air guide groove 611 is spline-shaped, which allows the venting groove 608 and the exhaust groove 601 to be connected through the air guide groove 611. At this time, the venting groove 603 on the sealing plate 602 has not yet detached from the sealing block 604. As the gas pressure in the incubator 3 gradually increases, the gas pressure overcomes the elastic force of the first elastic element 606, causing the venting groove 603 on the sealing plate 602 to detach from the sealing block 604. The exhaust groove 601 is connected to the incubator 3 through the exhaust groove 603, allowing the gas in the incubator 3 to be discharged into the constant temperature chamber 2 through the exhaust groove 601 and the venting groove 608.

[0059] When the incubator 3 is restored to normal pressure after exhausting, at this time, under the action of the first elastic member 606, the first elastic member 606 drives the sealing plate 602 to slide to the initial state through the positioning ring 605, so that the sealing block 604 extends into the exhaust groove 603 again, so that the exhaust groove 601 is sealed, and the sealing plate 602 is no longer inserted into the air guide groove 611. Under the action of the gas in the piston cavity 612, the piston rod 613 slides to the initial position along the piston cavity 612, and the intermittent plate 610 seals the exhaust groove 608 again, so that the gas in the incubator 3 can be discharged intermittently and according to the pressure, which is convenient to provide a stable gas environment for the growth of microorganisms, so that the microorganisms can be cultured comprehensively. It should be noted that one-way valves are arranged in the exhaust groove 608 and the connecting pipe 403, so that the gas flows from the connecting pipe 403 to the incubator 3 in one direction, and the gas flows from the incubator 3 to the exhaust groove 608 in one direction.

[0060] In summary, the swinging of the incubator 3 can intermittently press the piston rod 613, so that the exhaust groove 601 and the exhaust groove 608 are intermittently connected through the air guide groove 611, so that the gas in the incubator 3 can stably react with the microorganisms. When the gas pressure in the incubator 3 gradually increases, the gas pressure overcomes the elastic force of the first elastic member 606, so that the sealing block 604 is separated from the exhaust groove 603, which is convenient for the discharge of the gas in the incubator 3, and provides a stable gas environment for the growth of microorganisms. According to the different frequencies of the swinging of the incubator 3, the interval of the gas discharge in the incubator 3 will also increase, so that the pure gas entering the incubator 3 stably contacts and reacts with the microorganisms, which can effectively improve the effect of microorganism culture.

[0061] Example 3

[0062] The present example also provides a medical test microorganism culture method, comprising the following steps:

[0063] S1: First, place the culture dish or culture bottle containing microorganisms in the incubator 3, and then adjust the thermostat 2 to provide a stable growth environment.

[0064] S2: Then start the driving motor 8, which drives the microorganisms in the incubator 3 to shake, improving the efficiency of microorganism growth.

[0065] S3: Next, through the shaking of the incubator 3, the gas supplement mechanism 4 supplements gas to the incubator 3, and the adjusting mechanism 5 can adjust the gas amount according to different growth stages of microorganisms.

[0066] S4: Finally, through the shaking of the incubator 3, the exhaust mechanism 6 can be intermittently opened, so as to ensure the stability of the gas in the incubator 3, which is beneficial to the growth of microorganisms. After the culture is completed, the culture dish or culture bottle can be taken out.

[0067] The foregoing is considered as illustrative only of the principles of the application. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the application to the exact construction and practice described. Accordingly, all such variations and modifications are intended to be included within the scope of the application as defined in the following claims and the equivalents thereof.

Claims

1. A medical examination microorganism culture device, comprising a cabinet (1), a thermostat (2) is fixedly connected in the cabinet (1), characterized in that, The incubator (3) is slidably connected in the thermostat (2), the air supplementing mechanism (4) is connected between the incubator (3) and the thermostat (2), the air supplementing mechanism (4) is used for supplementing air into the incubator (3), the adjusting mechanism (5) is arranged in the air supplementing mechanism (4) and is used for adjusting the air supplementing amount of the air supplementing mechanism (4) into the incubator (3), the air exhausting mechanism (6) is arranged on the side, away from the air supplementing mechanism (4), of the incubator (3) and is used for intermittently adjusting the air density in the incubator (3); When the incubator (3) slides, the air supplementing amount of the air supplementing mechanism (4) can be adjusted by the adjusting mechanism (5), the air exhausting mechanism (6) can be intermittently opened and closed, the air pressure in the incubator (3) is increased, and the air in the incubator (3) is exhausted through the air exhausting mechanism (6); The air supplementing mechanism (4) comprises a connecting block (401) fixedly connected with the thermostat (2), an air supplementing channel (402) is arranged in the connecting block (401), one end of the air supplementing channel (402), close to the incubator (3), is communicated with the incubator (3) through a connecting pipe (403), and the other end of the air supplementing channel (402), away from the incubator (3), is fixedly connected with an air supplementing pipe (404), and the free end of the air supplementing pipe (404) extends out of the thermostat (2) and the cabinet (1) in sequence; The adjusting mechanism (5) comprises a containing groove (501) arranged in the connecting block (401), a rotating ring (502) is rotatably connected in the containing groove (501), the rotating ring (502) is fixedly connected with an adjusting plate (503) at one end, close to the connecting pipe (403), of the rotating ring (502), a plurality of arc-shaped grooves (504) are arranged on the adjusting plate (503) and are distributed in a circumferential direction at intervals, a plurality of baffle plates (505) corresponding to the arc-shaped grooves (504) are slidably connected in the connecting block (401), the plurality of baffle plates (505) can block the air supplementing channel (402), one end of each baffle plate (505), away from the air supplementing channel (402), is fixedly connected with a plug rod (506), and the free end of each plug rod (506) is inserted into the corresponding arc-shaped groove (504), when the adjusting plate (503) rotates, the plurality of plug rods (506) can slide in the direction of the air supplementing channel (402) along the arc-shaped grooves (504) synchronously; The incubator (3) is slidably connected in the thermostat (2), the air supplementing mechanism (4) is connected between the incubator (3) and the thermostat (2), the air supplementing mechanism (4) is used for supplementing air into the incubator (3), the adjusting mechanism (5) is arranged in the air supplementing mechanism (4) and is used for adjusting the air supplementing amount of the air supplementing mechanism (4) into the incubator (3), the air exhausting mechanism (6) is arranged on the side, away from the air supplementing mechanism (4), of the incubator (3) and is used for intermittently adjusting the air density in the incubator (3); The mass sensor (12) is arranged on the incubator (3) and is used for detecting the mass change of microorganisms in different growth periods, the area of the air supplementing channel (402) blocked by the plurality of baffle plates (505) is adjusted, so that the air supplementing amount of the air supplementing channel (402) can be automatically adjusted in different growth periods of microorganisms, the air supplementing amount is reasonably controlled, the gas demand of microorganisms in different growth periods can be met, and the incubator (3) is convenient to use.

2. The medical examination microorganism culture device according to claim 1, wherein: The circumference side of the swivel ring (502) is provided with an inclined guide groove (507), the connecting block (401) is slidably connected with a vertically extending guide rod (508), one side of the guide rod (508) close to the swivel ring (502) is fixedly connected with a jacking rod (509), the jacking rod (509) is slidably connected with the guide groove (507), one side of the incubator (3) close to the connecting block (401) is provided with a pressing block (510), the side of the pressing block (510) close to the connecting block (401) is a wedge surface, one end of the guide rod (508) close to the incubator (3) extends out of the connecting block (401) and can contact with the pressing block (510), the incubator (3) is connected with an electric telescopic rod (511), the output end of the electric telescopic rod (511) is fixedly connected with the pressing block (510), the guide rod (508) and the connecting block (401) are connected through a second elastic element (512).

3. The medical examination microorganism culture device according to claim 2, characterized in that: The exhaust mechanism (6) comprises an exhaust groove (601) provided on the incubator (3) and transversely penetrating, the exhaust groove (601) is slidably connected with a blocking plate (602), a plurality of circumferentially spaced gas outlet grooves (603) are provided on the blocking plate (602), a plurality of blocking blocks (604) corresponding to the gas outlet grooves (603) are fixedly connected in the exhaust groove (601), each blocking block (604) can extend into the corresponding gas outlet groove (603), a positioning ring (605) is fixedly connected in the exhaust groove (601), the positioning ring (605) and the blocking plate (602) are connected through a first elastic element (606).

4. The medical examination microorganism culture device according to claim 3, characterized in that: One side of the incubator (3) close to the exhaust groove (601) is fixedly connected with an auxiliary block (607), a gas discharge groove (608) in communication with the gas outlet groove (603) is provided on the auxiliary block (607), a slide (609) in communication with the gas discharge groove (608) is provided on the auxiliary block (607), an intermittent plate (610) for blocking the gas discharge groove (608) is slidably connected in the slide (609), a gas guide groove (611) capable of being located in the gas discharge groove (608) is provided on the intermittent plate (610), one end of the blocking plate (602) away from the incubator (3) can be inserted into the gas guide groove (611).

5. A medical examination microorganism culture device according to claim 4, characterized in that: A piston cavity (612) in communication with the slide (609) is provided in the auxiliary block (607), a piston rod (613) is slidably connected in the piston cavity (612), one end of the piston rod (613) away from the incubator (3) can contact with the inner wall of the incubator (3), when the piston rod (613) slides, the intermittent plate (610) can be driven to slide.

6. The medical examination microorganism culture device according to claim 5, characterized in that: The incubator (2) is fixedly connected with a supporting block (7) located below the incubator (3), the supporting block (7) is provided with a driving motor (8), the incubator (3) is fixedly connected with a limiting ring (9) on the side close to the driving motor (8), the output end of the driving motor (8) is coaxially fixedly connected with a disc (10), the disc (10) is eccentrically fixedly connected with a push rod (11), the push rod (11) extends into the limiting ring (9), and the quality sensor (12) is electrically connected with the driving motor (8) and the electric telescopic rod (511).

7. A medical examination microorganism culture device according to claim 6, characterized in that: The cabinet body (1) is hingedly connected with a cabinet door (13) capable of blocking the incubator (2), the opening of the incubator (3) is hingedly connected with a transparent plate (14) capable of blocking the opening, and the inner wall of the incubator (3) is provided with a clamping groove (15).

8. A medical examination microorganism culture method using the medical examination microorganism culture device according to claim 7, characterized by, The method comprises the following steps: S1: first, place the culture dish or culture bottle containing microorganisms in the incubator (3), and then adjust the incubator (2) to provide a stable growth environment; S2: then start the driving motor (8), the driving motor (8) drives the microorganisms in the incubator (3) to shake, improving the efficiency of microbial growth; S3: then, through the shaking of the incubator (3), the air supplementing mechanism (4) supplements gas into the incubator (3), and the adjusting mechanism (5) can adjust the amount of gas according to different growth stages of microorganisms; S4: finally, through the shaking of the incubator (3), the exhaust mechanism (6) can be intermittently opened, so as to ensure the stability of the gas in the incubator (3), which is beneficial to the growth of microorganisms, and after the culture is completed, the culture dish or culture bottle can be taken out.

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