An allergen detection kit

Abstract

The present invention relates to the technical field of test kits, and specifically discloses an allergen detection kit comprising, arranged in order from top to bottom, a front cover, a partition, a test box body, a deflection membrane, a cotton strip, and a rear cover. The front cover is provided with a sample loading hole, which is connected to a plurality of buckle grooves. The partition is provided with a plurality of openings. The test box body is provided with a plurality of test slots. The test slots, openings, and buckle grooves enclose a test chamber for accommodating a test membrane strip. The front cover is provided with an overflow channel connecting one side of the buckle groove. The partition and the test box body are provided with a first through hole and a second through hole, respectively, below the overflow channel. The test slot is provided with a third through hole and a fourth through hole, respectively, on the side proximal to and distal to the second through hole. The second and third through holes are sealed and connected at the bottom of the test box body by a deflection membrane. The cotton strip is provided below the fourth through hole. The test box body is provided with a gas hole. The present invention has a simple structure, high detection efficiency, and can achieve simultaneous detection of multiple allergens.

Description

Technical Field

[0001] The present invention belongs to the technical field of kits, and in particular relates to an allergen detection kit. Background Art

[0002] Allergic diseases are a type of disease caused by the body's immune system producing an abnormal immune response to harmless substances in the environment. Their incidence continues to rise worldwide.

[0003] Epidemiological surveys show that common allergic diseases include allergic rhinitis, asthma, conjunctivitis, eczema, food allergies, drug allergies, and anaphylactic shock. Food allergies are particularly prevalent among children and adolescents. Common food allergens include eggs, peanuts, milk, soy, wheat, tree nuts, fish, and crustaceans. These allergens can trigger the immune system to produce specific antibodies (such as IgE or IgG4), which in turn induce different types of allergic reactions. Therefore, the detection of specific antibodies is important for identifying food intolerances, guiding personalized dietary management, and clinical diagnosis.

[0004] Currently, the most commonly used allergen detection methods are traditional ELISA (enzyme-linked immunosorbent assay) and colloidal gold immunochromatography. While these methods have a solid foundation in clinical application, they still have significant shortcomings in practical testing. The traditional ELISA method has a complex testing process, requiring multiple incubations, washes, and enzymatic color development. This process is time-consuming and requires high operator skills and a robust laboratory environment. Furthermore, specialized equipment (such as a microplate reader) is required for result interpretation, making it difficult to rapidly respond to the demand for point-of-care testing. Furthermore, the traditional ELISA method is more suitable for centralized, batch testing settings, such as hospital laboratories or third-party medical laboratories. While it can improve overall testing efficiency and reduce labor burden, the high equipment investment and maintenance costs result in high marginal costs for single or small-volume tests, making it difficult to meet the practical needs of primary healthcare institutions, community health service centers, and home users for personalized, portable, and point-of-care testing. In contrast, the colloidal gold immunochromatography method offers advantages such as ease of use, the lack of complex instrumentation, and rapid result interpretation, making it a promising candidate for initial screening. However, this method has relatively low sensitivity, making it prone to false-negative or false-positive results, which can affect the accuracy of clinical judgment. Furthermore, when testing high-concentration samples, uneven liquid distribution can lead to a prozone phenomenon, where excess antigen inhibits the formation of antigen-antibody complexes, resulting in false-negative results. This necessitates appropriate sample dilution and retesting, increasing the complexity and time cost of testing.

[0005] In summary, existing allergen detection technologies still have obvious shortcomings in detection process, sensitivity, specificity, portability and cost control, which effectively limit their efficiency and reliability in clinical and practical applications.

[0006] Therefore, we propose an allergen detection kit to solve the above technical problems. Summary of the Invention

[0007] In order to solve the technical problems existing in the above-mentioned prior art, the present invention provides an allergen detection kit.

[0008] The technical solution adopted in the present invention is as follows:

[0009] An allergen detection kit comprises a front cover, a partition, a detection box body, a deflection membrane, a cotton strip and a rear cover, which are arranged in sequence from top to bottom. The front cover is provided with a through sample addition hole, which is connected to a plurality of buckle grooves arranged in parallel. The partition is provided with a plurality of openings. The top surface of the detection box body is provided with a plurality of detection slots. The positions of the detection slots, openings and buckle grooves correspond to each other one by one and enclose a detection chamber for accommodating the detection membrane strip. The front cover is provided with an overflow channel on one side of each buckle groove. The partition is provided with a plurality of openings. The plate and the detection box body are respectively provided with a first through hole and a second through hole in corresponding positions below the overflow channel. The detection groove is penetrated by a third through hole on the side close to the second through hole, and is penetrated by a fourth through hole on the side away from the second through hole. The bottom of the detection box body is provided with a first guide groove between the second through hole and the third through hole. The deflection membrane is attached to the bottom of the detection box body and covers and seals the second through hole, the first guide groove and the third through hole. The cotton strip is provided below the fourth through hole, and a gas hole is provided on the detection box body.

[0010] In a further technical solution, a diversion port is provided on the bottom surface of the front cover below the sample addition hole, and the diversion port is provided with a plurality of branched flow channels, and the branched flow channels are connected to the buckle groove.

[0011] In a further technical solution, the partition and the detection box body are respectively penetrated by a fifth through hole and a sixth through hole corresponding to the position of the sample addition hole, and a seventh through hole and an eighth through hole corresponding to the position of the diversion port. The bottom of the detection box body is provided with a second guide groove between the sixth through hole and the eighth through hole. The rear cover is attached to the bottom of the detection box body and covers and seals the sixth through hole, the second guide groove and the eighth through hole.

[0012] In a further technical solution, the gas hole includes an upper gas hole, and the upper gas hole is provided through the detection box body, and the rear cover is provided with a first gas exhaust hole corresponding to the position below the upper gas hole.

[0013] In a further technical solution, the gas hole also includes a lower air hole, and the lower air hole includes a first lower air hole and a second lower air hole, the first lower air hole and the second lower air hole are arranged through the detection box body, and the bottom of the detection box body is provided with a partition between the first lower air hole and the second lower air hole, the first lower air hole is located on one side of the second through hole, and the detection box body is provided with a second exhaust hole on the side wall on the side of the second lower air hole.

[0014] In a further technical solution, the front cover and the partition are both made of transparent plastic.

[0015] In a further technical solution, the preparation steps of the detection membrane strip are as follows:

[0016] First, the food components were grouped and the purified food-specific proteins were dissolved in buffer at a working concentration of 1 mg / mL;

[0017] Subsequently, a film spraying apparatus was used to evenly spray the buffer solution containing each group of dissolved food components onto the nitrocellulose membrane at certain intervals;

[0018] Finally, after drying, cut into membrane strips that match the size of the detection chamber and put them into aluminum foil bags with desiccant for later use.

[0019] In a further technical solution, the pH value of the buffer solution is 7.2, and the buffer solution includes 10 g / L BSA (bovine serum albumin, referred to as BSA hereinafter), 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.05 g / L TWEEN-20 (Tween-20, full name polyoxyethylene sorbitan monolaurate, used as a non-ionic surfactant, referred to as TWEEN-20 hereinafter), 0.15 mol / L NaCl solution, and 0.05 mol / L hydroxymethylaminomethane hydrochloric acid solution.

[0020] In a further technical solution, the detection steps of the allergen detection kit include the following:

[0021] Use a syringe to inject the sample to be tested from the sample injection hole, wait for the sample to be tested to incubate with the test membrane strip for 30 minutes, and then wash it three times with cleaning solution;

[0022] Use a syringe to add mouse anti-human IgG4 antibody reaction solution, incubate for 20 minutes, and then wash with washing solution;

[0023] Use a syringe to add horseradish peroxidase-labeled goat anti-mouse antibody reaction solution, incubate for 15 minutes, wash with cleaning solution, and then use a syringe to add substrate solution. Observe the band within 5 minutes;

[0024] Use a syringe to add the stop solution, stop the reaction and read the results: the color bands will show the corresponding results according to the antibody concentration;

[0025] The reagents used for the test include:

[0026] Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL mouse anti-human IgG4 antibody dissolved in pH 7.4, containing 5 mg / mL BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer;

[0027] A horseradish peroxidase-labeled goat anti-mouse antibody reaction solution is prepared by dissolving 0.1 mg / mL of the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution in a solution containing 5 mg / mL of BSA, 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.15 mol / L of NaCl solution, and 0.01 mol / L of phosphate buffer;

[0028] Substrate solution, TMB single-component colorimetric solution, was purchased from Suzhou Yaco Chemical Reagent Co., Ltd., catalog number D0022;

[0029] Stop solution, 0.5 mol / L H2SO4 solution;

[0030] The cleaning solution, with a pH value of 7.2, includes 10 g / L BSA, 0.1% Proclin 300 preservative of the total volume of the buffer, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer.

[0031] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0032] 1. The present invention does not require special instruments. It not only has a simple structure, reducing equipment investment and maintenance costs, but is also easy to carry and conduct rapid on-site testing. It has low requirements for the professional skills of the operator, effectively simplifies the testing process and operating steps, reduces the complexity and time cost of testing, and improves the accuracy and reliability of the test results. It can effectively meet the actual needs of primary medical institutions, community health service points or home users for "individualized testing", "portable testing" and "instant testing".

[0033] 2. The present invention relies on the design of the internal through holes and flow channels of the detection reagent kit to guide the liquid to flow along a predetermined path, realizing an automated liquid distribution and detection reaction process. The reagent flows through the top and bottom of the detection membrane strip in turn, realizing up and down two-way infiltration, ensuring sufficient contact and reaction between the liquid reagent and the allergen on the detection membrane strip, and effectively avoiding the "prozone phenomenon" caused by excessive sample concentration and uneven liquid distribution in traditional test strips, reducing the occurrence of false negative or false positive results, improving detection accuracy and stability, and reducing the waste of time and resources caused by repeated testing.

[0034] 3. The detection membrane strips in each detection chamber of the present invention can be sprayed with different types of purified food-specific proteins, which can realize the simultaneous detection of multiple food-related allergens, improve the detection efficiency, and effectively reduce the detection cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] The present invention will now be described by way of example with reference to the accompanying drawings, in which:

[0036] Figure 1 It is a structural schematic diagram of the present invention;

[0037] Figure 2 A schematic diagram of the bottom structure of the front cover of the present invention;

[0038] Figure 3 Schematic diagram of the structure of the separator of the present invention;

[0039] Figure 4 This is a schematic diagram of the top surface structure of the detection box body of the present invention;

[0040] Figure 5 Schematic diagram of the bottom structure of the detection box body of the present invention.

[0041] Figure markings: 1-front cover, 2-partition, 3-detection box body, 4-deflection membrane, 5-cotton strip, 6-rear cover, 7-sample loading hole, 8-buckle groove, 9-opening, 10-detection groove, 11-overflow channel, 12-first through hole, 13-second through hole, 14-third through hole, 15-fourth through hole, 16-first guide groove, 17-diversion port, 18-branching channel, 19-fifth through hole, 20-sixth through hole, 21-seventh through hole, 22-eighth through hole, 23-upper air hole, 24-first exhaust hole, 25-first lower air hole, 26-second lower air hole, 27-partition, 28-second exhaust hole, 29-second guide groove. DETAILED DESCRIPTION

[0042] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0043] Example:

[0044] See Figure 1-Figure 5 The present invention provides an allergen detection kit, comprising a front cover 1, a partition 2, a detection box body 3, a deflection membrane 4, a cotton strip 5 and a rear cover 6, which are arranged in sequence from top to bottom. The front cover 1 is provided with a through sample addition hole 7, and the sample addition hole 7 is connected to four buckle grooves 8 arranged in parallel. The partition 2 is provided with four openings 9. The top surface of the detection box body 3 is provided with four detection grooves 10. The positions of the detection grooves 10, the openings 9 and the buckle grooves 8 correspond to each other and enclose four independent detection chambers for accommodating the detection membrane strip. The front cover 1 is connected to an overflow channel 11 on one side of each buckle groove 8. The partition 2 is provided with four openings 9. The detection box body 3 is respectively provided with a first through hole 12 and a second through hole 13 in corresponding positions below the overflow channel 11. The detection slot 10 is penetrated by a third through hole 14 on the side close to the second through hole 13, and is penetrated by a fourth through hole 15 on the side away from the second through hole 13. The bottom of the detection box body 3 is provided with a first guide groove 16 between the second through hole 13 and the third through hole 14. The deflection membrane 4 is attached to the bottom of the detection box body 3 and covers and seals the second through hole 13, the first guide groove 16 and the third through hole 14. The cotton strip 5 is provided below the fourth through hole 15, and a gas hole is provided on the detection box body 3.

[0045] In this embodiment, see Figure 2 The bottom surface of the front cover 1 is connected to a diversion port 17 below the sample addition hole 7. The diversion port 17 is connected to four branch flow channels 18. The branch flow channels 18 are respectively connected to the adjacent buckle grooves 8.

[0046] In this embodiment, see Figure 1 、 Figure 3 、 Figure 4 and Figure 5 The partition plate 2 and the detection box body 3 are respectively provided with a fifth through hole 19 and a sixth through hole 20 corresponding to the position of the sample addition hole 7, and a seventh through hole 21 and an eighth through hole 22 corresponding to the position of the diversion port 17. The bottom of the detection box body 3 is provided with a second guide groove 29 between the sixth through hole 20 and the eighth through hole 22. The rear cover plate 6 is attached to the bottom of the detection box body 3 and covers and seals the sixth through hole 20, the second guide groove 29 and the eighth through hole 22.

[0047] In this embodiment, see Figure 1 、 Figure 4 and Figure 5 The gas holes include an upper gas hole 23 , which is provided through the detection box body 3 , and the rear cover 6 is provided with a first gas hole 24 corresponding to the position below the upper gas hole 23 .

[0048] In this embodiment, see Figure 4 and Figure 5 The gas holes also include lower air holes, which include a first lower air hole 25 and a second lower air hole 26. The first lower air hole 25 and the second lower air hole 26 are arranged through the detection box body 3. A partition 27 is provided between the first lower air hole 25 and the second lower air hole 26 at the bottom of the detection box body 3. The first lower air hole 25 is located on one side of the second through hole 13, and the detection box body 3 is provided with a second exhaust hole 28 on the side wall on the side of the second lower air hole 26.

[0049] In this embodiment, the front cover plate 1 and the partition plate 2 are both made of transparent plastic, which makes it easy to observe the reaction process and results.

[0050] In this embodiment, the preparation steps of the detection membrane strip are as follows:

[0051] First, the food components were grouped and the purified food-specific proteins were dissolved in buffer at a working concentration of 1 mg / mL;

[0052] Subsequently, a film spraying apparatus was used to evenly spray the buffer solution containing each group of dissolved food components onto the nitrocellulose membrane at certain intervals;

[0053] Finally, after drying, cut into membrane strips that match the size of the detection chamber and put them into aluminum foil bags with desiccant for later use.

[0054] In this embodiment, the pH value of the buffer solution is 7.2, and the buffer solution includes 10 g / L BSA, 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution, and 0.05 mol / L hydroxymethylaminomethane hydrochloric acid solution.

[0055] In this embodiment, the detection steps of the allergen detection kit include the following:

[0056] Use a syringe to inject the sample to be tested from the sample injection hole 7, wait for the sample to be tested to incubate with the detection membrane strip for 30 minutes, and then use the cleaning solution to wash 3 times;

[0057] Use a syringe to add mouse anti-human IgG4 antibody reaction solution, incubate for 20 minutes, and then wash with washing solution;

[0058] Use a syringe to add horseradish peroxidase-labeled goat anti-mouse antibody reaction solution, incubate for 15 minutes, wash with cleaning solution, and then use a syringe to add substrate solution (TMB color development solution) and observe the band within 5 minutes;

[0059] Use a syringe to add the stop solution, stop the reaction and read the results: the color bands will show the corresponding results according to the antibody concentration;

[0060] The reagents used for the test include:

[0061] Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL mouse anti-human IgG4 antibody dissolved in pH 7.4, containing 5 mg / mL BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer;

[0062] A horseradish peroxidase-labeled goat anti-mouse antibody reaction solution is prepared by dissolving 0.1 mg / mL of the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution in a solution containing 5 mg / mL of BSA, 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.15 mol / L of NaCl solution, and 0.01 mol / L of phosphate buffer;

[0063] Substrate solution, TMB single-component colorimetric solution, was purchased from Suzhou Yaco Chemical Reagent Co., Ltd., catalog number D0022;

[0064] Stop solution, 0.5 mol / L H2SO4 solution;

[0065] The cleaning solution, with a pH value of 7.2, includes 10 g / L BSA, 0.1% Proclin 300 preservative of the total volume of the buffer, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer.

[0066] The allergen detection kit of the present invention, comprised of a front cover 1, a partition 2, a detection box body 3, a deflection membrane 4, a cotton strip 5, and a rear cover 6, forms a compact, easy-to-use detection product suitable for a variety of allergen detection. The front cover 1 is provided with a sample injection port 7 for adding test samples, reaction solutions, color development solutions, cleaning solutions, and other reagents. Once liquid enters through the sample injection port 7, the design of the internal through-holes and flow channels of the detection kit guides the liquid along a predetermined path, automating the liquid distribution and detection reaction process. This significantly reduces operational complexity and is particularly suitable for use in primary healthcare settings or by non-professionals.

[0067] Specifically, the flow path of the liquid in the test kit is as follows:

[0068] First, the test kit is placed horizontally, and liquid is added to the sample injection port 7 via a syringe. The liquid then flows downward through the fifth through-hole 19 and the sixth through-hole 20, entering the closed diversion channel formed by the rear cover 6 and the bottom of the test box body 3. In this channel, the liquid flows from the sixth through-hole 20 along the second diversion groove 29 to the eighth through-hole 22. Subsequently, the liquid flows upward through the seventh through-hole 21 and the diversion port 17, where it is diverted to various branching channels 18 and enters each test chamber. After the liquid reaches the test chamber, it is located above the test membrane strip due to the spacing effect of the partition 2. While soaking the upper part of the test membrane strip, it diffuses along the gap between the test membrane strip and the buckle groove 8 to the overflow channel 11. Then, the excess liquid flows downward from the overflow channel 11, flowing through the first through-hole 12 and the second through-hole 13, and enters the closed diversion channel formed by the deflection membrane 4 and the bottom of the test box body 3. In this flow channel, the liquid flows from the second through hole 13 along the first guide groove 16 to the third through hole 14. Afterwards, the liquid flows upward along the third through hole 14 and enters the detection chamber again. At this time, the liquid is located below the detection membrane strip, and while infiltrating the lower part of the detection membrane strip, it diffuses to the fourth through hole 15 along the gap between the detection groove 10 and the detection membrane strip. Finally, the cotton strip 5 below the fourth through hole 15 absorbs the residual liquid to prevent the residual liquid from interfering with the test results. In this process, the liquid infiltrates in both directions from above and below the detection membrane strip, ensuring sufficient contact and reaction between the liquid reagent and the allergen on the detection membrane strip, effectively avoiding the "front band phenomenon" caused by excessive sample concentration and uneven liquid distribution in traditional test strips, reducing the occurrence of false negative or false positive results, improving detection accuracy and stability, and reducing the waste of time and resources caused by repeated testing.

[0069] It is worth mentioning that the number of detection chambers of this detection kit can be designed according to the detection requirements. In this embodiment, the detection membrane strips in each detection chamber can be sprayed with different types of purified food-specific proteins, such as dairy products, seafood, nuts, grains, etc., so as to achieve simultaneous detection of multiple food-related allergens, improve detection efficiency, and effectively reduce detection costs. In addition, the detection kit is reasonably arranged with gas holes, a first exhaust hole 24 and a second exhaust hole 28. During the flow of liquid, the internal and external air pressures can be automatically adjusted to ensure that the liquid flows smoothly along the established path, avoid fluid path abnormalities or bubble interference caused by air blockage, and further ensure the accuracy of the test results. In the actual detection step, after the addition reaction of each reagent is completed, it is cleaned with a cleaning solution. The purpose is to remove the excess reaction substances in the previous step to prevent them from interfering with the subsequent reaction steps, thereby ensuring the specificity of the entire detection process and the reliability of the results.

[0070] Compared to existing allergen detection methods, this test kit does not require specialized instruments. It not only has a simple structure, reducing equipment investment and maintenance costs, but is also easy to carry and perform rapid on-site testing. It requires low operator skills, effectively simplifies the testing process and operating steps, reduces testing complexity and time costs, and improves the accuracy and reliability of test results. It can effectively meet the actual needs of primary care institutions, community health service points, or home users for "personalized testing," "portable testing," and "instant testing." Furthermore, this test kit is suitable not only for the detection of IgG4 antibodies, but also for the detection of IgE antibodies. Simply replace the mouse anti-human IgG4 antibody reaction solution in the test system with a mouse anti-human IgE antibody reaction solution; the rest of the operating procedures remain the same. The mouse anti-human IgE antibody reaction solution is prepared by dissolving 0.1 mg / mL of mouse anti-human IgE antibody in a pH 7.4 solution containing 5 mg / mL of BSA, 0.1% of the total buffer volume of Proclin 300 preservative, 0.15 mol / L of NaCl solution, and 0.01 mol / L of phosphate buffer. This solution can be widely used in allergen-related preclinical screening, personalized health assessments, and home self-testing scenarios, demonstrating excellent applicability, scalability, and industrialization prospects.

[0071] The above descriptions are merely embodiments of the present invention and are not intended to limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made using the contents of the present invention description and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.

Claims

1. An allergen detection kit, characterized in that: The invention comprises a front cover (1), a partition (2), a detection box body (3), a deflection membrane (4), a cotton strip (5) and a rear cover (6) which are sequentially arranged from top to bottom, wherein the front cover (1) is provided with a through sample addition hole (7), the sample addition hole (7) is connected to a plurality of buckle grooves (8) arranged in parallel, the partition (2) is provided with a plurality of openings (9), the top surface of the detection box body (3) is provided with a plurality of detection grooves (10), the positions of the detection grooves (10), the openings (9) and the buckle grooves (8) correspond to each other, and enclose a detection chamber for accommodating the detection membrane strip, the front cover (1) is connected to an overflow channel (11) on one side of each buckle groove (8), the partition (2) and the detection box body (3) are connected at the overflow channel. A first through hole (12) and a second through hole (13) are provided at corresponding positions below the flow channel (11), a third through hole (14) is provided on the side of the detection slot (10) close to the second through hole (13), and a fourth through hole (15) is provided on the side away from the second through hole (13), a first guide groove (16) is provided at the bottom of the detection box body (3) between the second through hole (13) and the third through hole (14), the deflection membrane (4) is attached to the bottom of the detection box body (3), and covers and seals the second through hole (13), the first guide groove (16) and the third through hole (14), the cotton strip (5) is provided below the fourth through hole (15), and a gas hole is provided on the detection box body (3); The bottom surface of the front cover plate (1) is provided with a diversion port (17) below the sample addition hole (7), and the diversion port (17) is provided with a plurality of bifurcated flow channels (18) in communication with each other. The bifurcated flow channels (18) are connected to the buckle groove (8); The partition plate (2) and the detection box body (3) are respectively provided with a fifth through hole (19) and a sixth through hole (20) corresponding to the position of the sample addition hole (7), and a seventh through hole (21) and an eighth through hole (22) corresponding to the position of the diversion port (17). The bottom of the detection box body (3) is provided with a second guide groove (29) between the sixth through hole (20) and the eighth through hole (22). The rear cover plate (6) is attached to the bottom of the detection box body (3) and covers and seals the sixth through hole (20), the second guide groove (29) and the eighth through hole (22). The method for using the allergen detection kit is as follows: First, the test kit is placed horizontally, and liquid is added to the sample addition hole (7) through a syringe. The liquid flows downward through the fifth through hole (19) and the sixth through hole (20) in sequence, and enters the closed guide channel formed by the rear cover (6) and the bottom of the test box body (3); in the channel, the liquid flows from the sixth through hole (20) along the second guide groove (29) to the eighth through hole (22); then, the liquid flows upward through the seventh through hole (21) and the diversion port (17), and the liquid is diverted to each branch flow channel (18) through the diversion port (17) and enters each test chamber respectively; after the liquid reaches the test chamber, it is located above the test membrane strip due to the spacing effect of the partition (2), and while infiltrating the upper part of the test membrane strip, it flows along the space between the test membrane strip and the buckle groove (8) The excess liquid then flows downward from the overflow channel (11), sequentially flows through the first through hole (12) and the second through hole (13), and enters the closed guide channel formed by the deflection membrane (4) and the bottom of the detection box body (3); in the channel, the liquid flows from the second through hole (13) along the first guide groove (16) to the third through hole (14); thereafter, the liquid flows upward along the third through hole (14) and enters the detection chamber again; at this time, the liquid is located below the detection membrane strip, and while soaking the lower part of the detection membrane strip, it diffuses along the gap between the detection groove (10) and the detection membrane strip to the fourth through hole (15); finally, the cotton strip (5) below the fourth through hole (15) absorbs the residual liquid to prevent the residual liquid from interfering with the detection result.

2. An allergen detection kit according to claim 1, characterized in that: The gas holes include an upper gas hole (23), the upper gas hole (23) is provided through the detection box body (3), and the rear cover plate (6) is provided with a first gas exhaust hole (24) at a corresponding position below the upper gas hole (23).

3. An allergen detection kit according to claim 2, characterized in that: The gas hole further comprises lower gas holes, and the lower gas holes comprise a first lower gas hole (25) and a second lower gas hole (26). The first lower gas hole (25) and the second lower gas hole (26) are arranged through the detection box body (3). A partition (27) is provided between the first lower gas hole (25) and the second lower gas hole (26) at the bottom of the detection box body (3). The first lower gas hole (25) is located on one side of the second through hole (13). The detection box body (3) is provided with a second gas exhaust hole (28) on a side wall on one side of the second lower gas hole (26).

4. The allergen detection kit according to claim 1, characterized in that: The front cover (1) and the partition (2) are both made of transparent plastic.

5. The allergen detection kit according to claim 1, characterized in that: The preparation steps of the detection membrane strip are as follows: First, the food components were grouped and the purified food-specific proteins were dissolved in buffer at a working concentration of 1 mg / mL; Subsequently, a film spraying apparatus was used to evenly spray the buffer solution containing each group of dissolved food components onto the nitrocellulose membrane at certain intervals; Finally, after drying, the membrane is cut into strips that match the size of the detection chamber and placed in an aluminum foil bag with desiccant for later use.

6. The allergen detection kit according to claim 5, characterized in that: The pH value of the buffer solution is 7.2, and the buffer solution includes 10 g / L BSA, 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution, and 0.05 mol / L hydroxymethylaminomethane hydrochloric acid solution.

7. The allergen detection kit according to claim 1, characterized in that: The detection steps of the allergen detection kit include the following: Use a syringe to inject the sample to be tested from the sample injection hole (7), wait for the sample to be tested to incubate with the detection membrane strip for 30 minutes, and then use the cleaning solution to wash 3 times; Use a syringe to add mouse anti-human IgG4 antibody reaction solution, incubate for 20 minutes, and then wash with washing solution; Use a syringe to add horseradish peroxidase-labeled goat anti-mouse antibody reaction solution, incubate for 15 minutes, wash with cleaning solution, then use a syringe to add substrate solution, and observe the band within 5 minutes; Use a syringe to add the stop solution, stop the reaction and read the results: the color bands will show the corresponding results according to the antibody concentration; The reagents used for the test include: Mouse anti-human IgG4 antibody reaction solution, using 0.1 mg / mL mouse anti-human IgG4 antibody dissolved in pH 7.4, containing 5 mg / mL BSA, 0.1% Proclin 300 preservative accounting for the total volume of the buffer, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer; A horseradish peroxidase-labeled goat anti-mouse antibody reaction solution is prepared by dissolving 0.1 mg / mL of the horseradish peroxidase-labeled goat anti-mouse antibody reaction solution in a solution containing 5 mg / mL of BSA, 0.1% of the total volume of the buffer solution as a Proclin 300 preservative, 0.15 mol / L of NaCl solution, and 0.01 mol / L of phosphate buffer; Substrate solution, TMB single-component colorimetric solution; Stop solution, 0.5 mol / L H2SO4 solution; The cleaning solution, with a pH value of 7.2, includes 10 g / L BSA, 0.1% Proclin 300 preservative of the total volume of the buffer, 0.05 g / L TWEEN-20, 0.15 mol / L NaCl solution and 0.01 mol / L phosphate buffer.

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