Whitening essence and preparation method thereof

Abstract

The invention relates to the technical field of skin care products, in particular to whitening essence and a preparation method thereof, and the whitening essence comprises the following components: phenylethyl resorcinol, tetrahydrocurcumin, plant polyphenol and a liquid preparation. According to the whitening essence and the preparation method thereof, generation of skin melanin can be effectively inhibited, and the whitening essence has good anti-inflammatory and anti-allergic effects, can prevent pigmentation caused by inflammation, and also has anti-saccharification and skin color uniformizing effects.

Description

Technical Field

[0001] This application relates to the technical field of skin care products, and particularly to a whitening essence and a method for making the same. Background Art

[0002] Melanin formation is a complex biochemical process. Melanin starts from tyrosine. The first two steps are oxidation processes, both of which require tyrosinase to participate, turning into dopa and dopaquinone, and then through isomerization and polymerization reactions, turning into melanin. Melanin is initially in the dermis layer and then transfers to the stratum corneum. Under normal circumstances, melanin is evenly distributed, which can reduce the damage to the skin when exposed to sunlight.

[0003] For example, Chinese Patent with application number 201711243991.6 discloses a whitening essence, which is prepared from the following raw materials in parts by weight: 1 - 3 parts of organic white tea extract, 5 - 9 parts of alpine plant extract, 10 - 35 parts of silicone oil, 6 - 12 parts of glycerol, 1 - 3 parts of silk protein, 5 - 9 parts of peony flower essential oil, 1 - 5 parts of betaine, 0.5 - 3.5 parts of tridecyl alcohol trimellitate, 0.5 - 4.5 parts of xanthan gum, 5 - 11 parts of allantoin, 0.1 - 0.6 parts of sodium hyaluronate, 0.8 - 2.6 parts of glycerol polyether, 1 - 6 parts of niacinamide, 0.2 - 0.8 parts of hydrogenated lecithin, 0.2 - 1.6 parts of seaweed essence, 0.3 - 1.2 parts of hexylene glycol, 0.2 - 0.8 parts of essence, and 85 - 165 parts of deionized water.

[0004] Regarding the above - mentioned related technologies, the inventor believes that there are the following defects:

[0005] Common whitening essences have many production ingredients and complex production processes, resulting in relatively high production costs, which is not conducive to market promotion. Moreover, they are difficult to effectively inhibit melanin formation, with poor and incomplete whitening effects, so there is room for improvement. Summary of the Invention

[0006] This application provides a whitening essence and a method for making the same to improve the following technical problems:

[0007] Common whitening essences have many production ingredients and complex production processes, resulting in relatively high production costs, which is not conducive to market promotion. Moreover, they are difficult to effectively inhibit melanin formation, with poor and incomplete whitening effects.

[0008] In the first aspect, this application provides a whitening essence, adopting the following technical solution:

[0009] A whitening essence, comprising the following components in parts by weight:

[0010]

[0011] In an implementable technical solution of the present application, the phenethyl resorcinol is a nano-active substance with a particle size of less than 30 nm developed by a nano-delivery system, and the phenethyl resorcinol is stably encapsulated in the nano-carrier.

[0012] In an implementable technical solution of the present application, the plant polyphenol is a single component or a compound of multiple components among the following materials: extract of Glycyrrhiza glabra root, resveratrol, soy isoflavones, extract of Magnolia officinalis bark, extract of citrus fruit.

[0013] In an implementable technical solution of the present application, the plant polyphenol is a compound of the following three material components: extract of Glycyrrhiza glabra root, resveratrol, extract of Magnolia officinalis bark, and the extract of Glycyrrhiza glabra root contains an alcohol-soluble component: glabridin.

[0014] In an implementable technical solution of the present application, it includes the following components in parts by weight:

[0015]

[0016] In an implementable technical solution of the present application, the liquid preparation is a single component or a compound of multiple components among the following materials: water, glycerol, propylene glycol, Limnanthes alba seed oil, squalane, jojoba oil.

[0017] In the second aspect, the present application provides a method for preparing the above-mentioned whitening essence, adopting the following technical solution:

[0018] A method for preparing a whitening essence, comprising the following steps:

[0019] Step 1: Prepare the required weight of raw materials according to the contained percentages;

[0020] Step 2: Preparation of the antioxidant plant extract mother liquor:

[0021] Divide the plant polyphenol into three equal parts and add them to glycerol with a mass ratio of 3-5 times the concentration of more than 95% three times, stir at a speed of 100-200 r / min, with an interval of 0.5-1 hour each time, and heat at a temperature of 40-50 °C;

[0022] Step 3: Preparation of the whitening effect mother liquor:

[0023] Prepare the corresponding mass of tetrahydrocurcumin and phenethyl resorcinol, add them to a 1:1 mixture of glycerol / propylene glycol with a mass ratio of 3-5 times, stir well and heat, with a heating temperature of 70-75 °C, a stirrer speed of 200-400 r / min, and a stirring time of 1-2 hours.

[0024] Step 4: Feed the antioxidant plant extract mother liquor obtained in Step 2 and the skin-whitening efficacy mother liquor obtained in Step 3 into a mixing tank for thorough mixing. In this step, the mixing temperature is 15-20°C, the rotation speed of the mixer is 200-300 r / min, and the mixing time is 3-5 hours to obtain the skin-whitening essence.

[0025] In summary, the working principle and beneficial technical effects of this application are as follows:

[0026] Phenylethyl resorcinol is a highly efficient new skin whitening agent derived from silver pine, with extremely strong potential skin-whitening efficacy. Taking advantage of the similarity in structure between the raw material and tyrosine, tyrosinase cannot distinguish and competitively inhibit the formation of melanin, and its strong antioxidant property can reduce the already formed melanin. In vitro experiments have also proven that it is dozens or even hundreds of times stronger than VC, and its skin-whitening ability has been clinically confirmed, comparable to that of hydroquinone. By using a nano-delivery system (NDS) to develop nano-actives with a particle size of less than 30 nm, phenylethyl resorcinol can be stably encapsulated in the nano-carrier, which can greatly increase the absorption efficiency and bioavailability.

[0027] The unique physicochemical and biological properties of plant polyphenols make them natural skin protectants against UV-induced damage (inflammation, photoaging, carcinogenesis). Many important phenylalanine metabolites in the human body (estrogen, thyroxine, tyrosine, dopamine) are very similar to plant polyphenols derived from phenylalanine. Therefore, when plant polyphenols are applied to the human body, they easily enter and replace or compete with the metabolic pathways of endogenous phenylalanine metabolites, which provides a theoretical basis for plant polyphenol skin care products for skin whitening, anti-aging, anti-acne, anti-hair loss, and anti-hirsutism. In terms of the anti-inflammatory effect most relevant to promoting wound healing, it not only attributes to the antioxidant, free radical scavenging, and metal chelating properties of plant polyphenols, but more importantly, the effect depends on the interaction between plant polyphenols and resident (keratinocytes) and recruited (granulocytes, lymphocytes, and dendritic cells) inflammatory cells in the skin. The plant polyphenol raw material has strong tyrosinase inhibitory activity, can effectively inhibit the production of skin melanin, has good anti-inflammatory and anti-allergic effects, prevents pigment deposition caused by inflammation, and at the same time has anti-glycation and skin tone evenness effects.

[0028] Tetrahydrocurcumin is derived from the roots of Indian medicinal turmeric, with a purity of ≥95%. Tetrahydrocurcumin can resist ultraviolet radiation, inhibit the generation of free radicals, inhibit the production of melanin, and block the factors causing inflammation. In addition, the inhibition of free radicals by tetrahydrocurcumin and the inhibition of various inflammatory factors such as lipoxygenase, collagenase, and hyaluronidase reflect the potential anti-aging effect of super pure whitening essence; Tetrahydrocurcumin can also inhibit the cascade reaction of arachidonic acid, inhibit the generation of free radicals, inhibit tyrosinase, and inhibit melanin. Practice has proved that tetrahydrocurcumin can significantly brighten the skin tone, enhance the skin gloss, improve dullness and yellowness, increase skin blood color and rosiness (this is particularly prominent in middle-aged women), and even at an addition amount of 1-2%, tetrahydrocurcumin will not cause any discomfort or allergy to sensitive skin, but instead makes the effect more prominent. Description of the Drawings

[0029] To more clearly illustrate the technical solutions in the embodiments of the present application, the following will briefly introduce the drawings required for use in the description of the embodiments. Obviously, the following drawings are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0030] Figure 1 It is a comparison table of the experimental groups in the embodiments of the present application.

[0031] Figure 2 It is a bar chart of the experimental results in the embodiments of the present application.

[0032] Figure 3 It is a comparison chart of cell nuclei of the experimental group and the control group in the embodiments of the present application under drug intervention conditions.

[0033] Figure 4 It is a comparison chart of the cytoskeletons of the experimental group and the control group in the embodiments of the present application under drug intervention conditions.

[0034] Figure 5 It is a comparison chart of melanin of the experimental group and the control group in the embodiments of the present application under drug intervention conditions.

[0035] Figure 6 It is a comparison chart of the combined states of the experimental group and the control group in the embodiments of the present application under drug intervention conditions.

[0036] Figure 7 It is a facial observation distribution chart of Subject A before the test in the embodiments of the present application.

[0037] Figure 8 It is a facial observation distribution chart of Subject A after the test in the embodiments of the present application.

[0038] Figure 9It is the VISIA score difference table of 20 subjects in the embodiments of the present application.

[0039] Figure 10 It is the VISIA imaging analysis bar chart in the embodiments of the present application. Detailed implementation manners

[0040] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present application more clear and understandable, the present application will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and are not used to limit the present application.

[0041] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element.

[0042] It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present application.

[0043] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present application, "a plurality of" means two or more unless otherwise specifically defined.

[0044] The following will further describe the present application with reference to the attached Figures 1-10 The present application will be further described in detail.

[0045] The embodiments of the present application disclose a whitening essence, which comprises the following components in parts by weight:

[0046]

[0047] In the embodiments of the present application, the phenethyl resorcinol is a nano-active substance with a particle size less than 30 nm developed by a nano-delivery system, and the phenethyl resorcinol is stably encapsulated in a nano-carrier.

[0048] The plant polyphenols are a single component or a combination of multiple components of the following materials: glycyrrhiza glabra root extract, resveratrol, soy isoflavones, magnolia bark extract, and citrus fruit extract.

[0049] Specifically, the plant polyphenols are a compound of the following three material components: Glycyrrhiza glabra root extract, resveratrol, and Magnolia officinalis bark extract, and the Glycyrrhiza glabra root extract contains an alcohol-soluble component: glabridin.

[0050] In this embodiment, the liquid preparation is a mixture of water, glycerin and propylene glycol. In other embodiments, it can also be water, glycerin, propylene glycol, white meadowfoam seed oil, squalane or jojoba oil, or a mixture of multiple materials including water, glycerin, propylene glycol, white meadowfoam seed oil, squalane or jojoba oil.

[0051] In the present application, a preferred embodiment is provided, the whitening essence comprises the following components in parts by weight:

[0052]

[0053] The present application also provides a method for preparing the whitening essence as described above, comprising the following steps:

[0054] Step 1: Prepare the required weight of raw materials according to the percentage contained;

[0055] Step 2: Preparation of antioxidant plant extract mother solution:

[0056] The plant polyphenols are divided into three equal parts, and added into glycerol with a concentration of 3-5 times the mass ratio of 95% or more in three times, and stirred at a speed of 100-200 r / min, with an interval of 0.5-1 hour each time, and a heating temperature of 40-50° C., wherein the plant polyphenols are raw materials for antioxidant plant extracts, and the raw materials for antioxidant plant extracts include extracts from the root of Glycyrrhiza glabra, resveratrol, and extracts from the bark of Magnolia officinalis;

[0057] Step 3: Preparation of whitening effect mother solution:

[0058] Prepare corresponding amounts of tetrahydrocurcumin and phenethylresorcinol, add them into a 1:1 mixture of glycerol / propylene glycol in a ratio of 3 to 5 times by mass, stir and heat thoroughly, the heating temperature is 70 to 75° C., the stirrer speed is 200 to 400 r / min, and the stirring time is 1 to 2 hours.

[0059] Step 4: Add the antioxidant plant extract mother solution obtained in step 2 and the whitening effect mother solution obtained in step 3 into a stirring tank and mix them thoroughly. In this step, the stirring temperature is 15-20°C, the stirring speed is 200-300r / min, and the stirring time is 3-5 hours to obtain a whitening essence.

[0060] In summary, the working principle and beneficial technical effects of the present application are as follows:

[0061] Phenylethyl resorcinol is a highly efficient new skin whitening agent derived from silver fir. It has extremely strong potential whitening effects. By virtue of the similarity in structure between the raw material and tyrosine, tyrosinase cannot distinguish and competitively inhibits the production of melanin, and its strong antioxidant property reduces the already produced melanin. In vitro experiments have also proven that it is dozens or even hundreds of times stronger than VC, and its whitening ability has been clinically confirmed to be comparable to that of hydroquinone. The nano-active substance with a particle size less than 30 nm developed by using the nano-delivery system (NDS) can stably encapsulate phenylethyl resorcinol in the nano-carrier, which can greatly increase the absorption efficiency and bioavailability.

[0062] The unique physical, chemical, and biological properties of plant polyphenols make them natural skin protectants against UV-induced damage (inflammation, photoaging, carcinogenesis). Many important phenylalanine metabolites in the human body (estrogen, thyroxine, tyrosine, dopamine) are very similar to plant polyphenols derived from phenylalanine. Therefore, it is easy for plant polyphenols to enter and substitute or compete with the metabolic pathways of endogenous phenylalanine metabolites when applied to the human body, which provides a theoretical basis for plant polyphenol skin care products for whitening, anti-aging, anti-acne, anti-hair loss, and anti-hirsutism. In terms of the anti-inflammatory effect most relevant to promoting wound healing, it not only attributes to the antioxidant, free radical scavenging, and metal chelating properties of plant polyphenols, but more importantly, the effect depends on the interaction between plant polyphenols and resident (keratinocytes) and recruited (granulocytes, lymphocytes, and dendritic cells) inflammatory cells in the skin. The raw material of plant polyphenols has strong tyrosinase inhibitory activity, which can effectively inhibit the production of skin melanin. Its good anti-inflammatory and anti-allergic effects can prevent pigment deposition caused by inflammation, and at the same time has the effects of anti-glycation and skin tone uniformity.

[0063] Tetrahydrocurcumin is derived from the roots of Indian medicinal turmeric, with a purity of ≥95%. Tetrahydrocurcumin can resist ultraviolet radiation, inhibit the generation of free radicals, inhibit the generation of melanin, and block the factors causing inflammation. In addition, the inhibition of free radicals by tetrahydrocurcumin, and the inhibition of various inflammatory factors such as lipoxygenase, collagenase, and hyaluronidase reflect the potential anti-aging effect of the ultra-pure whitening agent. Tetrahydrocurcumin can also inhibit the cascade reaction of arachidonic acid, inhibit the generation of free radicals, inhibit tyrosinase, and inhibit melanin. Practice has proved that tetrahydrocurcumin can greatly brighten the skin tone, enhance the skin gloss, improve dullness and yellowness, and increase skin blood color and rosiness (this is particularly prominent for middle-aged women). Tetrahydrocurcumin will not cause any discomfort or allergy even at an addition amount of 1-2% for sensitive skin, but instead makes the effect more prominent.

[0064] To explore the inhibitory effect of the ratio of tetrahydrocurcumin and phenethyl resorcinol on melanin production in melanocytes and provide data support for further expanding the application of tetrahydrocurcumin and phenethyl resorcinol in the cosmetic field, this example also provides the following experimental methods:

[0065] (1) Melanin inhibition test

[0066] Determination of intracellular melanin content: Take cells in the logarithmic growth phase, digest them with trypsin, and make them into 1.2×10 5 cells / mL, inoculate 2 mL per well in a 6-well culture plate, and culture in a CO incubator for 24 h. After adding the drug equal dilution solution, continue to culture for 48 h, wash 2 times with PBS (pH 7.4), add 100 μL of cell lysate to each well, lyse on ice for 30 min, centrifuge at 12,000 r / min for 15 min, discard the supernatant, add 150 μL of 1 mol / L NaOH solution containing 10% DMSO to the precipitate, and keep it in a constant temperature water bath at 80 °C for 30 min. Measure the absorbance value at 405 nm with an enzyme-linked immunosorbent assay (ELISA) reader, and calculate the melanin content according to the standard curve set with artificial melanin as the melanin standard.

[0067]

[0068] (2) Immunofluorescence staining of melanin distribution under drug intervention

[0069] Perform immunofluorescence staining on the melanocytes after drug intervention in the above group 7 with the melanoma antibody Melan-A (1:500). The red color is mouse anti-Melan-A (1:500), the green color is the Phalloidin fluorescent protein antibody, and the blue color is DAPI nuclear staining.

[0070] (3) VISIA clinical observation: Clean the face of 20 subjects before the initial evaluation, and use the VISIA skin detector to record the conditions of the red area, spots, texture, etc. of the skin before treatment. Instruct the subjects to evenly apply 2 mL of the whitening essence of this application to the face only twice a day during the test period. Do a good job in sun protection after application. After continuous use for 4 weeks, return for a follow-up visit, and the VISIA skin detector records the conditions of the red area, spots, texture, etc. of the skin after use.

[0071] Please refer to Figure 1 and Figure 2 , the experimental results are as follows:

[0072] It can be seen that Group 7 (5:1) has the strongest effect on inhibiting melanogenesis in melanocytes, with melanin reduced to 17.25%, and the ability to inhibit melanogenesis is about 4 times that of single tetrahydrocurcumin. Among Groups 1-7, as the content of phenethyl resorcinol increases, the ability of the drug solution to inhibit melanin gradually increases. In Group 8, a decrease in the ability to inhibit melanin is observed, and partial apoptosis and cytoplasmic condensation are also observed, with an increase in the concentration of melanin in melanocytes in vivo, which may be related to apoptosis caused by too high a concentration of the added drug, suggesting that a higher drug ratio may produce certain cytotoxicity and reduce the ability to inhibit melanin.

[0073] (2) Immunofluorescence staining of melanin distribution under drug intervention

[0074] Please refer to Figures 3-6 , it can be seen that the synthesis of melanin under drug intervention in Group 7 is significantly reduced, indicating that the drug can significantly inhibit melanin formation, and there are no significant morphological changes in the cytoskeleton and cell nucleus, indicating that the drug has little effect on the morphology of the cytoskeleton and cell nucleus.

[0075] (3) VISIA clinical observation

[0076] Please refer to Figure 7 , before the test of Subject A, the spots were 47%, the texture was 46%, the ultraviolet spots were 6%, the brown spots were 23%, the red area was 6%, and the pores were 4%.

[0077] Please refer to Figure 8 , after the test of Subject A, the spots were 54% (7% increase compared with before), the texture was 77%

[0078] (31% increase compared with before), the ultraviolet spots were 36% (30% increase compared with before), the brown spots were 38% (15% increase compared with before), the red area was 43% (37% increase compared with before), and the pores were 5% (1% increase compared with before). It shows that the facial spots of Subject A are reduced, the skin fineness is increased, the ultraviolet spots and brown spots are both reduced, and the red area of the skin is reduced.

[0079] Combined with Figure 9 and Figure 10 , after the test of 20 subjects, it can be seen that the spots are increased by 28.65% compared with before, the texture is increased by 31.45% compared with before, the ultraviolet spots are increased by 39.65% compared with before, the brown spots are increased by 24.65% compared with before, the red area is increased by 45% compared with before, and the pores are increased by 9% compared with before.

[0080] The above are only the preferred embodiments of the present application, and are not intended to limit the present application. Any modifications, equivalent replacements, and improvements made within the spirit and principles of the application shall be included in the protection scope of the present application.

Claims

1. A whitening essence, characterized in that, The composition comprises the following components in parts by weight:

2. The whitening essence according to claim 1, wherein The phenethyl resorcinol is a nano-active substance with a particle size less than 30 nm developed by a nano-transmission system, and the phenethyl resorcinol is stably encapsulated in a nano-carrier.

3. The whitening essence according to claim 1, wherein The plant polyphenols are a single component or a composite of multiple components of the following materials: glycyrrhiza glabra root extract, resveratrol, soybean isoflavones, magnolia bark extract, and citrus fruit extract.

4. The whitening essence according to claim 3, characterized in that, The plant polyphenols are a composite of the following three material components: glycyrrhiza glabra root extract, resveratrol, and magnolia officinalis bark extract, and the glycyrrhiza glabra root extract contains an alcohol-soluble component: glabridin.

5. The whitening essence according to claim 4, characterized in that, The composition comprises the following components in parts by weight:

6. The whitening essence according to claim 1, wherein The liquid preparation is a single component or a compound of multiple components of the following materials: water, glycerin, propylene glycol, meadowfoam seed oil, squalane, and jojoba oil.

7. A method for preparing a whitening essence according to any one of claims 1 to 6, characterized in that, The following steps are involved: Step 1: Prepare the required weight of raw materials according to the percentage contained; Step 2: Preparation of antioxidant plant extract mother solution: Divide the plant polyphenols into three equal parts, add them into glycerol with a concentration of 3-5 times the mass ratio of 95% or more three times, stir at a speed of 100-200r / min, each time interval is 0.5-1 hour, and the heating temperature is 40-50°C; Step 3: Preparation of whitening effect mother solution: Prepare corresponding amounts of tetrahydrocurcumin and phenethylresorcinol, add them into a 1:1 mixture of glycerol / propylene glycol in a ratio of 3 to 5 times by mass, stir and heat thoroughly, the heating temperature is 70 to 75° C., the stirrer speed is 200 to 400 r / min, and the stirring time is 1 to 2 hours. Step 4: Add the antioxidant plant extract mother solution obtained in step 2 and the whitening effect mother solution obtained in step 3 into a stirring tank and mix them thoroughly. In this step, the stirring temperature is 15-20°C, the stirring speed is 200-300r / min, and the stirring time is 3-5 hours to obtain a whitening essence.

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