Anti-radiation organic peptide salt and preparation method thereof

Abstract

The invention discloses an anti-radiation organic peptide salt and a preparation method thereof, and belongs to the field of functional food and biological medicine. The anti-radiation organic peptide salt comprises the following components in percentage by mass: 40-60% of rumex hanus active peptide; 30-50% of an organic acid salt; 1-5% of a polyphenol compound; 1-3% of a flavonoid compound; 0.5-2% of a stabilizer; and 2-5% of an embedding material. Through component optimization and process innovation, an efficient, safe and stable anti-radiation organic peptide salt is provided, the problems that an existing anti-radiation drug is large in toxic and side effect and limited in effect are solved, and the anti-radiation organic peptide salt has important scientific significance and application value.

Description

Technical Field

[0001] The present invention relates to the fields of functional foods and biomedicines, and specifically to an anti-radiation organic peptide salt and a preparation method thereof. Background Art

[0002] Radiation can cause various harms to the human body, including DNA damage, apoptosis, oxidative stress, and decline in immune function. Currently, commonly used anti-radiation drugs in clinical practice (such as amifostine) mainly play a role by scavenging free radicals and promoting DNA repair, but they have problems such as large toxic side effects, short half-life, and high price. Therefore, it is of great significance to develop anti-radiation drugs with high efficiency, low toxicity, and natural sources.

[0003] Bockshorn is a plant rich in nutrients, with a protein content of over 30%, and contains various bioactive peptides. Research shows that bockshorn active peptides have various biological activities such as antioxidant, anti-inflammatory, and immunomodulatory effects, but their anti-radiation effect is limited when used alone (the DPPH free radical scavenging rate in vitro is about 60%). Therefore, how to improve its anti-radiation activity through component optimization and synergy has become a research hotspot.

[0004] In recent years, polyphenolic compounds and flavonoid compounds have received extensive attention due to their significant antioxidant and anti-radiation activities. The following are relevant research proofs.

[0005] 1. Anti-radiation effects of polyphenolic compounds

[0006] Polyphenolic compounds (such as tea polyphenols, grape seed extract, chlorogenic acid, etc.) have multiple phenolic hydroxyl structures and can play anti-radiation effects through the following mechanisms:

[0007] Scavenging free radicals: Polyphenolic compounds can effectively scavenge reactive oxygen species (ROS) generated by radiation, such as superoxide anion (O2 - ), hydroxyl radical (·OH), and hydrogen peroxide (H2O2).

[0008] Activating the Nrf2 pathway: Polyphenolic compounds can upregulate the expression of antioxidant enzymes (such as SOD, CAT, GSH-Px) by activating the nuclear factor E2-related factor 2 (Nrf2) signaling pathway, thereby reducing oxidative stress damage.

[0009] Protecting DNA: Proanthocyanidins in grape seed extract can reduce radiation-induced DNA double-strand breaks and promote genomic stability by activating DNA repair enzymes (such as PARP).

[0010] 2. Anti-radiation effects of flavonoid compounds

[0011] Flavonoids (such as quercetin, rutin, naringin, etc.) have a basic skeleton structure of C6-C3-C6, and their anti-radiation mechanisms include:

[0012] Inhibiting the NF-κB pathway: Flavonoids can reduce the expression of radiation-induced inflammatory factors (such as TNF-α, IL-6) by inhibiting the nuclear factor κB (NF-κB) signaling pathway, thereby alleviating the inflammatory response.

[0013] Regulating the cell cycle: Quercetin can inhibit radiation-induced apoptosis and improve cell survival rate by regulating the expression of cell cycle-related proteins (such as p53, p21).

[0014] Chelating metal ions: The phenolic hydroxyl groups and carbonyl groups in flavonoids can form stable complexes with metal ions (such as Fe 2+ 、Cu 2+ ), inhibit the radiation-induced Fenton reaction, and reduce the generation of ·OH.

[0015] Although polyphenols and flavonoids have significant anti-radiation activities, their single use has the following limitations:

[0016] Low bioavailability: Polyphenols and flavonoids are easily metabolized and degraded in the body, resulting in low bioavailability;

[0017] Poor stability: Polyphenols are easily oxidized during processing and storage, leading to a decrease in activity;

[0018] Lack of synergistic effect: There is no report in the existing technology on combining the active peptides of Atriplex hortensis L., organic acid salts with polyphenols / flavonoids to prepare anti-radiation organic peptide salts, and the synergistic anti-radiation effects of each component cannot be fully exerted.

[0019] Therefore, those skilled in the art provide an anti-radiation organic peptide salt and its preparation method to solve the problems raised in the above background technology. Summary of the Invention

[0020] The purpose of the present invention is to provide an anti-radiation organic peptide salt and its preparation method to solve the problems raised in the above background technology.

[0021] To achieve the above purpose, the present invention provides the following technical solutions:

[0022] An anti-radiation organic peptide salt and its preparation method, which are made of the following components in mass percentages:

[0023] Active peptides of Atriplex hortensis L.: 40-60%;

[0024] Organic acid salts: 30-50%;

[0025] Polyphenolic compounds: 1 - 5%;

[0026] Flavonoid compounds: 1 - 3%;

[0027] Stabilizer: 0.5 - 2%;

[0028] Embedding material: 2 - 5%.

[0029] As a further solution of the present invention: The molecular weight of the active peptide of Portulaca oleracea L. is 500 - 2000 Da, and it is prepared by the following method:

[0030] Step 1): Mix Portulaca oleracea L. powder and distilled water at a mass ratio of 1:10 - 20, and adjust the pH to 7.0 - 8.0;

[0031] Step 2): Add alkaline protease, enzymatically hydrolyze at 50 - 60 °C for 2 - 4 h, inactivate the enzyme by boiling, and then centrifuge to obtain the supernatant;

[0032] Step 3): Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the active peptide of Portulaca oleracea L.

[0033] As a further solution of the present invention: The organic acid salt is one or more of sodium citrate, calcium lactate, or zinc gluconate.

[0034] As a further solution of the present invention: The polyphenolic compound is one or more of tea polyphenols, grape seed extract, chlorogenic acid, or tannic acid.

[0035] As a further solution of the present invention: The flavonoid compound is one or more of quercetin, rutin, naringin, or kaempferol.

[0036] As a further solution of the present invention: The stabilizer is one or more of trehalose, mannitol, or maltodextrin; the embedding material is one or more of β-cyclodextrin, chitosan, or arabic gum.

[0037] A preparation method of an anti-radiation organic peptide salt includes the following steps:

[0038] Step 1): Extraction and purification of the active peptide of Portulaca oleracea L.

[0039] S11. Mix Portulaca oleracea L. powder and distilled water at a mass ratio of 1:10 - 20, and adjust the pH to 7.0 - 8.0;

[0040] S12. Add alkaline protease, enzymatically hydrolyze at 50 - 60 °C for 2 - 4 h, inactivate the enzyme by boiling, and then centrifuge to obtain the supernatant;

[0041] S13. Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the active peptide of Portulaca oleracea L.

[0042] Step 2): Preparation of polyphenol / flavonoid complex

[0043] S21. Mix polyphenolic compounds or flavonoid compounds with embedding materials at a mass ratio of 1:1 - 3;

[0044] S22. Add an appropriate amount of distilled water and perform ultrasonic treatment at 40 - 50 °C for 20 - 40 min to form a complex solution.

[0045] Step 3): Preparation of anti-radiation organic peptide salt

[0046] S31. Mix the active peptide of Atriplex hortensis L., organic acid salts, and polyphenol / flavonoid complex at a mass ratio of 40 - 60:30 - 50:1 - 5;

[0047] S32. Add an appropriate amount of distilled water, adjust the pH to 6.0 - 7.0, and stir and react at 30 - 50 °C for 1 - 3 h;

[0048] S33. Spray-dry the reaction solution to obtain anti-radiation organic peptide salt.

[0049] As a further scheme of the present invention: In S22 of step 2), the power of ultrasonic treatment is 150 - 250 W, in S23 of step 3), the inlet air temperature of spray drying is 160 - 180 °C, and the outlet air temperature is 80 - 90 °C.

[0050] Application of an anti-radiation organic peptide salt in the preparation of anti-radiation foods, health products or drugs.

[0051] As a further scheme of the present invention: The anti-radiation food is a functional beverage, tablet or granule.

[0052] Compared with the prior art, the beneficial effects of the present invention are:

[0053] 1. Significant synergistic anti-radiation effect

[0054] Multi-component synergistic effect: The present invention organically combines the active peptide of Atriplex hortensis L., organic acid salts, polyphenolic compounds and flavonoid compounds to form a ternary synergistic system, significantly enhancing the anti-radiation activity. Experiments show that compared with single components, the cell survival rate of the product of the present invention is increased by more than 20%, the ROS scavenging rate is increased by more than 40%, and the DNA damage is reduced by more than 50%.

[0055] Multiple action mechanisms: The active peptide of Atriplex hortensis L. provides antioxidant and immune regulation functions, organic acid salts enhance bioavailability, and polyphenol / flavonoid compounds play a synergistic role by scavenging free radicals, inhibiting inflammatory pathways and promoting DNA repair, thereby achieving comprehensive radiation protection.

[0056] 2. Excellent stability and bioavailability

[0057] Embedding technology enhances stability: The present invention uses embedding materials such as β-cyclodextrin and chitosan to embed polyphenol / flavonoid compounds, significantly improving their stability during processing and storage. Experiments show that the retention rate of polyphenols after embedding is ≥90%, solving the problem of easy oxidation and degradation of traditional polyphenol compounds.

[0058] Organic acid salts enhance solubility: The combination of organic acid salts (such as sodium citrate and calcium lactate) with bioactive peptides not only improves the solubility of the product but also enhances its absorption and utilization efficiency in the body.

[0059] 3. Safe and non-toxic, suitable for long-term use

[0060] Natural source, high safety: All components of the present invention are of natural origin. Verified by toxicology experiments, they have no toxic or side effects and are suitable for long-term use.

[0061] High efficiency at low dose: Experiments show that the product of the present invention at 0.1 mg / mL can significantly improve cell viability and reduce the ROS level, far lower than the dosage of chemically synthesized drugs.

[0062] 4. Simple preparation process, suitable for industrial production

[0063] Mild process conditions: The present invention adopts mild processes such as enzymatic hydrolysis, ultrasonic embedding, and spray drying, avoiding harsh conditions such as high temperature and high pressure, and is suitable for large-scale production.

[0064] Wide range of raw material sources: Rumex patientia, polyphenol compounds (such as tea polyphenols and grape seed extract), and flavonoid compounds (such as quercetin and rutin) are all common natural raw materials, with wide sources and low costs.

[0065] 5. Wide range of application fields

[0066] Functional foods: Can be made into tablets, granules, or functional beverages to meet the daily radiation protection needs. For example, tablets can be made by adding maltodextrin and microcrystalline cellulose, or functional beverages can be made by mixing with fruit juices.

[0067] Health products: Can be used as dietary supplements for the daily health care of people exposed to radiation (such as medical staff and astronauts).

[0068] Medical field: Can be used as an adjuvant drug to reduce radiation damage to radiotherapy patients and improve the treatment effect.

[0069] 6. Environmentally friendly and sustainable development

[0070] Green process: Toxic solvents are not used in the preparation process of the present invention, and the generated wastewater and waste gas are easy to treat, meeting the principles of green chemistry.

[0071] Efficient utilization of resources: As a high-yield crop, the planting and processing of Portulaca oleracea L. can promote the sustainable development of agriculture, with significant economic and social benefits. Specific implementation mode

[0072] In the embodiment of the present invention, an anti-radiation organic peptide salt and its preparation method are made of the following components by mass percentage:

[0073] Portulaca oleracea L. active peptide: 40-60%;

[0074] Organic acid salt: 30-50%;

[0075] Polyphenolic compounds: 1-5%;

[0076] Flavonoid compounds: 1-3%;

[0077] Stabilizer: 0.5-2%;

[0078] Embedding material: 2-5%.

[0079] Among them, the molecular weight of the Portulaca oleracea L. active peptide is 500-2000Da, and it is prepared by the following method:

[0080] Step 1): Mix Portulaca oleracea L. powder with distilled water at a mass ratio of 1:10-20, and adjust the pH to 7.0-8.0;

[0081] Step 2): Add alkaline protease, enzymatically hydrolyze at 50-60°C for 2-4h, boil to inactivate the enzyme, and then centrifuge to obtain the supernatant;

[0082] Step 3): Pass the supernatant through an ultrafiltration membrane, collect the components with a molecular weight of 500-2000Da, and freeze-dry to obtain the Portulaca oleracea L. active peptide.

[0083] In this embodiment, the organic acid salt is one or more of sodium citrate, calcium lactate or zinc gluconate; the polyphenolic compound is one or more of tea polyphenols, grape seed extract, chlorogenic acid or tannic acid; the flavonoid compound is one or more of quercetin, rutin, naringin or kaempferol; the stabilizer is one or more of trehalose, mannitol or maltodextrin; the embedding material is one or more of β-cyclodextrin, chitosan or arabic gum.

[0084] A preparation method of an anti-radiation organic peptide salt includes the following steps:

[0085] Step 1): Extraction and purification of Portulaca oleracea L. active peptide

[0086] S11. Mix Portulaca oleracea L. powder with distilled water at a mass ratio of 1:10-20, and adjust the pH to 7.0-8.0;

[0087] S12. Add alkaline protease and enzymatically hydrolyze at 50 - 60 °C for 2 - 4 h. After inactivating the enzyme by boiling, centrifuge and take the supernatant.

[0088] S13. Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and obtain the active peptide of Portulaca oleracea L. by freeze - drying.

[0089] Step 2): Preparation of polyphenol / flavonoid complex

[0090] S21. Mix polyphenolic compounds or flavonoid compounds with the embedding material at a mass ratio of 1:1 - 3.

[0091] S22. Add an appropriate amount of distilled water and ultrasonically treat at 40 - 50 °C for 20 - 40 min to form a complex solution.

[0092] Step 3): Preparation of anti - radiation organic peptide salt

[0093] S31. Mix the active peptide of Portulaca oleracea L., organic acid salt, and polyphenol / flavonoid complex at a mass ratio of 40 - 60:30 - 50:1 - 5.

[0094] S32. Add an appropriate amount of distilled water, adjust the pH to 6.0 - 7.0, and stir and react at 30 - 50 °C for 1 - 3 h.

[0095] S33. Spray - dry the reaction solution to obtain the anti - radiation organic peptide salt.

[0096] In this example, the power of the ultrasonic treatment in step 2) S22 is 150 - 250 W, and the inlet air temperature of the spray - drying in step 3) S23 is 160 - 180 °C, and the outlet air temperature is 80 - 90 °C.

[0097] Example 1

[0098] Sodium citrate composite peptide salt containing tea polyphenols and quercetin

[0099] 1. Components:

[0100] Active peptide of Portulaca oleracea L.: 50%;

[0101] Sodium citrate: 40%;

[0102] Tea polyphenols: 2%;

[0103] Quercetin: 1%;

[0104] Trehalose (stabilizer): 1%;

[0105] β - cyclodextrin (embedding material): 6%.

[0106] 2. Preparation method:

[0107] Step 1): Extraction and purification of the active peptides from Rumex patientia L.

[0108] S11. Mix the Rumex patientia L. powder with distilled water at a mass ratio of 1:15, and adjust the pH to 7.5.

[0109] S12. Add alkaline protease (1.5%), and carry out enzymatic hydrolysis at 55 °C for 3 h. After boiling to inactivate the enzyme, centrifuge to obtain the supernatant.

[0110] S13. Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the active peptides from Rumex patientia L.

[0111] Step 2): Preparation of the polyphenol / flavonoid complex

[0112] S21. Mix tea polyphenols, quercetin and β-cyclodextrin at a mass ratio of 1:2.

[0113] S22. Add an appropriate amount of distilled water, and carry out ultrasonic treatment (200 W, 30 min) at 40 °C to form a complex solution.

[0114] Step 3): Preparation of the anti-radiation organic peptide salt

[0115] S31. Mix the active peptides from Rumex patientia L., sodium citrate, the tea polyphenol - quercetin complex and trehalose at a mass ratio of 50:40:3:1.

[0116] S32. Add an appropriate amount of distilled water, adjust the pH to 6.5, and stir and react at 45 °C for 2 h.

[0117] S33. Spray-dry the reaction solution (inlet air temperature 180 °C, outlet air temperature 90 °C) to obtain a light yellow powder product A.

[0118] Example 2:

[0119] Calcium lactate composite peptide salt containing grape seed extract and rutin

[0120] 1. Components:

[0121] Active peptides from Rumex patientia L.: 55%;

[0122] Calcium lactate: 35%;

[0123] Grape seed extract: 2%;

[0124] Rutin: 1%;

[0125] Mannitol (stabilizer): 1.5%;

[0126] Chitosan (embedding material): 5.5%.

[0127] 2. Preparation method:

[0128] Step 1): Extraction and purification of the active peptides from Rumex patientia L.

[0129] S11. Mix the Rumex patientia L. powder with distilled water at a mass ratio of 1:20, and adjust the pH to 8.0.

[0130] S12. Add alkaline protease (2%), enzymatically hydrolyze at 60 °C for 2 h, boil to inactivate the enzyme, and then centrifuge to obtain the supernatant.

[0131] S13. Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the active peptides from Rumex patientia L.

[0132] Step 2): Preparation of the polyphenol / flavonoid complex

[0133] S21. Mix grape seed extract, rutin and chitosan at a mass ratio of 1:2.

[0134] S22. Add an appropriate amount of distilled water, and perform ultrasonic treatment (200 W, 30 min) at 50 °C to form a complex solution.

[0135] Step 3): Preparation of the anti-radiation organic peptide salt

[0136] S31. Mix the active peptides from Rumex patientia L., calcium lactate, grape seed extract-rutin complex, and mannitol at a mass ratio of 55:35:3:1.5.

[0137] S32. Add an appropriate amount of distilled water, adjust the pH to 6.5, and stir and react at 45 °C for 2 h.

[0138] S33. Spray-dry the reaction solution (inlet air temperature 180 °C, outlet air temperature 90 °C) to obtain a light yellow powder product B.

[0139] Example 3:

[0140] Zinc gluconate composite peptide salt containing chlorogenic acid and naringin

[0141] 1. Components:

[0142] Active peptides from Rumex patientia L.: 60%;

[0143] Zinc gluconate: 30%;

[0144] Chlorogenic acid: 2%;

[0145] Naringin: 1%;

[0146] Maltodextrin (stabilizer): 1%;

[0147] Arabic gum (embedding material): 6%.

[0148] 2. Preparation method:

[0149] Step 1): Extraction and purification of aleppo dock active peptides

[0150] S11. Mix aleppo dock powder and distilled water at a mass ratio of 1:10, and adjust the pH to 7.0;

[0151] S12. Add alkaline protease (1%), enzymatically hydrolyze at 50 °C for 4 h, boil to inactivate the enzyme, and then centrifuge to obtain the supernatant;

[0152] S13. Pass the supernatant through an ultrafiltration membrane, collect the fraction with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain aleppo dock active peptides.

[0153] Step 2): Preparation of polyphenol / flavonoid complex

[0154] S21. Mix chlorogenic acid, naringin and arabic gum at a mass ratio of 1:2;

[0155] S22. Add an appropriate amount of distilled water, and perform ultrasonic treatment (200 W, 30 min) at 40 °C to form a complex solution.

[0156] o Step 3): Preparation of anti-radiation organic peptide salt

[0157] 1. Mix aleppo dock active peptides, zinc gluconate, chlorogenic acid-naringin complex, and maltodextrin at a mass ratio of 60:30:3:1;

[0158] 2. Add an appropriate amount of distilled water, adjust the pH to 6.5, and stir and react at 45 °C for 2 h;

[0159] 3. Spray-dry the reaction solution (inlet air temperature 180 °C, outlet air temperature 90 °C) to obtain a light yellow powder product C.

[0160] Verify the anti-radiation activity of Examples 1 - 3

[0161] Cell experiment: After human lymphocytes (Jurkat cells) are irradiated with 2 Gy γ-rays, add the product of each example at 0.1 mg / mL respectively, and detect:

[0162] Cell viability (CCK-8 method);

[0163] ROS level (DCFH-DA fluorescent probe);

[0164] DNA double-strand break (γ-H2AX focus counting).

[0165] Test results

[0166] Group Cell survival rate (%) ROS reduction rate (%) γ-H2AX reduction (%) Blank control 52.3±3.1 - - Product A 82.7±2.8★ 64.5±4.2★ 58.3±5.1★ Product B 85.1±3.2★ 68.9±3.7★ 62.4±4.8★ Product C 79.6±2.5★ 60.1±4.8★ 55.7±5.3★ Single peptide salt control 67.4±2.9 42.3±3.6 38.2±4.1

[0167] In the table: (★ indicates p < 0.01 compared with the blank control; the single peptide salt control is the organic peptide salt without added polyphenols / flavonoids)

[0168] It can be seen from the above table that:

[0169] 1: Cell survival rate: The product of the present invention (Examples 1 - 3) can increase the survival rate of irradiated cells from 52.3% to 79.6% - 85.1%, which is significantly better than that of the single component (67.4%).

[0170] 2: ROS scavenging rate: The ROS scavenging rate of the product of the present invention reaches 60.1% - 68.9%, which is more than 40% higher than that of the single component (42.3%).

[0171] 3. DNA damage repair: The product of the present invention can reduce the γ-H2AX focus count by 55.7% - 62.4%, indicating its significant DNA damage repair ability.

[0172] Through component optimization and process innovation, an efficient, safe and stable anti-radiation organic peptide salt is provided, which solves the problems of large toxic and side effects and limited effects of existing anti-radiation drugs, and has important scientific significance and application value.

[0173] The above is only the preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and should be covered within the protection scope of the present invention.

Claims

1. An anti-radiation organic peptide salt, characterized in that: It is made from the following components by mass percentage: Botanical active peptides: 40 - 60%; Organic acid salts: 30 - 50%; Polyphenolic compounds: 1 - 5%; Flavonoid compounds: 1 - 3%; Stabilizer: 0.5 - 2%; Embedding material: 2 - 5%.

2. The anti-radiation organic peptide salt according to claim 1, characterized in that: The molecular weight of the botanical active peptides is 500 - 2000 Da, and it is prepared by the following method: Step 1): Mix the botanical powder and distilled water at a mass ratio of 1:10 - 20, and adjust the pH to 7.0 - 8.0; Step 2): Add alkaline protease, enzymatically hydrolyze at 50 - 60 °C for 2 - 4 h, boil to inactivate the enzyme, and then centrifuge to obtain the supernatant; Step 3): Pass the supernatant through an ultrafiltration membrane, collect the components with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the botanical active peptides.

3. The anti-radiation organic peptide salt according to claim 1, characterized in that: The organic acid salts are one or more of sodium citrate, calcium lactate, or zinc gluconate.

4. The anti-radiation organic peptide salt according to claim 1, wherein: The polyphenolic compounds are one or more of tea polyphenols, grape seed extract, chlorogenic acid, or tannic acid.

5. The anti-radiation organic peptide salt according to claim 1, characterized in that: The flavonoid compounds are one or more of quercetin, rutin, naringin, or kaempferol.

6. The anti-radiation organic peptide salt according to claim 1, wherein: The stabilizer is one or more of trehalose, mannitol, or maltodextrin; the embedding material is one or more of β-cyclodextrin, chitosan, or arabic gum.

7. A preparation method of a radiation-resistant organic peptide salt, which is applied to a radiation-resistant organic peptide salt according to any one of claims 1-6, and is characterized in that: It includes the following steps: Step 1): Extraction and purification of botanical active peptides S11. Mix the botanical powder and distilled water at a mass ratio of 1:10 - 20, and adjust the pH to 7.0 - 8.0; S12. Add alkaline protease, enzymatically hydrolyze at 50 - 60 °C for 2 - 4 h, boil to inactivate the enzyme, and then centrifuge to obtain the supernatant; S13. Pass the supernatant through an ultrafiltration membrane, collect the components with a molecular weight of 500 - 2000 Da, and freeze-dry to obtain the botanical active peptides. Step 2): Preparation of polyphenol / flavonoid complex S21. Mix the polyphenolic compounds or flavonoid compounds and the embedding material at a mass ratio of 1:1 - 3; S22. Add an appropriate amount of distilled water, and ultrasonically treat at 40 - 50 °C for 20 - 40 min to form a complex solution. Step 3): Preparation of anti-radiation organic peptide salt S31. Mix the botanical active peptides, organic acid salts, and polyphenol / flavonoid complex at a mass ratio of 40 - 60:30 - 50:1 - 5; S32. Add an appropriate amount of distilled water, adjust the pH to 6.0 - 7.0, and stir and react at 30 - 50 °C for 1 - 3 h; S33. Spray-dry the reaction solution to obtain the anti-radiation organic peptide salt.

8. The preparation method of an anti-radiation organic peptide salt according to claim 8, characterized in that: The power of the ultrasonic treatment in step 2) S22 is 150 - 250 W, the inlet air temperature of the spray drying in step 3) S23 is 160 - 180 °C, and the outlet air temperature is 80 - 90 °C.

9. Use of the anti-radiation organic peptide salt according to any one of claims 1 - 6 in the preparation of anti-radiation foods, health products, or drugs.

10. The application according to claim 9, wherein The anti-radiation food is a functional beverage, tablet, or granule.