Liquid chromatography column chromatography separation equipment

Abstract

The present invention discloses a liquid chromatography column chromatography separation device, specifically relates to the field of liquid chromatography columns, including a support frame, a chromatography separation component is provided on the support frame, the chromatography separation component includes a column tube, the bottom end of the column tube is provided with an end cap 2, the top of the end cap 2 is provided with a filter screen plate, the stationary phase is poured into the column tube through the top of the column tube, and the filter screen plate is used to filter the stationary phase. The present invention sets a rotating disk in the piston, the cavity and the outside are in a closed state, the bottom of the piston can apply a stable pressure to the stationary phase, ensure that the stationary phase can be evenly and tightly filled in the column tube, the through hole 2 is connected to the through hole 1, and the mobile phase liquid is discharged to the stationary phase through a plurality of through holes 1 evenly arranged at equal intervals, and the dispersed multiple through holes 1 can more evenly distribute the mobile phase, reduce the impact on the stationary phase, and enable the mobile phase to fully participate in the separation process, so that different components can be well separated and the separation efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid chromatography columns, and more particularly to a liquid chromatography column chromatography separation device. Background Art

[0002] A liquid chromatography column is a chromatographic column filled with a stationary phase. When a sample mixture enters the column along with the mobile phase, the sample components migrate at different speeds within the column due to differences in distribution coefficients and adsorption capacities between the stationary and mobile phases. This allows for separation of the different components within the column. Liquid chromatography columns are widely used in pharmaceutical analysis, the food industry, and environmental monitoring. To accommodate more sample, thereby reducing the number of injections and improving separation efficiency, a larger diameter column is typically used.

[0003] In the liquid chromatography column of the prior art, a mobile phase delivery tube is arranged in the piston, and the discharge end of the delivery tube is located at the bottom of the piston. When in use, the stationary phase is first added to the column tube, and then a linear drive device is used to drive the piston to compress the stationary phase in the column tube, and the piston applies constant pressure to the stationary phase to ensure that the stationary phase is tightly packed and improve the separation efficiency of the chromatographic column. The mobile phase is then delivered through the delivery tube. In order to overcome the resistance of the mobile phase to the stationary phase particles, pressure needs to be applied to deliver the mobile phase. When the mobile phase passes through the stationary phase, different components are separated in the column tube.

[0004] However, due to the large diameter of the column tube, the position where the mobile phase passes through the stationary phase is relatively single, that is, the mobile phase is unevenly distributed in the stationary phase, and only part of the stationary phase can effectively participate in the separation process. The migration path of the mobile phase in the stationary phase becomes complicated, and different components cannot be well separated, resulting in low separation efficiency. Summary of the Invention

[0005] The present invention provides a liquid chromatography column chromatography separation device to solve the problem that: due to the large diameter of the column tube of the existing liquid chromatography column chromatography separation device, the position where the mobile phase passes through the stationary phase is relatively single, that is, the mobile phase is unevenly distributed in the stationary phase, only part of the stationary phase can effectively participate in the separation process, the migration path of the mobile phase in the stationary phase becomes complicated, and different components cannot be well separated, resulting in low separation efficiency.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solution: a liquid chromatography column chromatography separation device, comprising a support frame, a chromatography separation component provided on the support frame, the chromatography separation component comprising a column tube, a second end cap provided at the bottom end of the column tube, a filter sieve plate provided at the top of the second end cap, a stationary phase is poured into the column tube through the top end of the column tube, and the filter sieve plate is used to filter the stationary phase;

[0007] The support frame is provided with an extrusion mechanism, which includes a second linear drive, and a column pressure maintaining mechanism is provided at the output end of the second linear drive. The column pressure maintaining mechanism includes a piston, a cavity is provided inside the piston, a through hole 1 is provided at the bottom of the piston, a rotating disk is rotatably provided on the inner wall of the bottom of the cavity, a through hole 2 is provided on the rotating disk, and a mobile phase delivery pipe is fixedly connected to the cavity, and the mobile phase delivery pipe is used to deliver the mobile phase into the cavity;

[0008] The output end of the second linear drive is used to drive the piston to move along the length of the column tube. When the through hole one and the through hole two are misaligned, the piston applies a constant pressure on the stationary phase by moving vertically downward in the column tube. When the through hole one and the through hole two are connected, the mobile phase in the cavity enters the stationary phase to achieve separation.

[0009] In a preferred embodiment, a material removal mechanism is provided in the piston, and the material removal mechanism includes a movable shaft, an arc-shaped hole is provided on the rotating disk, the movable shaft is slidably set in the arc-shaped hole and the through hole, a guide groove is provided on the inner wall of the arc-shaped hole, and a connecting seat is fixedly provided on the movable shaft, and the connecting seat is slidably set in the guide groove.

[0010] In a preferred embodiment, a through hole four is opened on the rotating disk, and a through hole three is opened on the bottom inner wall of the cavity. Through hole three is adapted to through hole four, the bottom end of through hole three is connected to through hole one, and the bottom end of through hole three is also adapted to the bottom end of the movable shaft.

[0011] In a preferred embodiment, a through hole five is opened on the arc-shaped hole, and the through hole five is connected to the through hole four. The through hole five is used to transport the mobile phase in the arc-shaped hole to the through hole four.

[0012] In a preferred embodiment, two support plates are fixedly provided on the support frame, mounting holes are opened on the support plates, auxiliary rings are fixedly provided on the support plates, the auxiliary rings are concentrically arranged with the mounting holes, fixing rings are placed in the two auxiliary rings, the diameter of the fixing rings is larger than the aperture of the mounting holes, two connecting rings are fixedly provided on the column tube, and the fixing rings are fixed to the corresponding connecting rings by bolts.

[0013] In a preferred embodiment, the chromatographic separation component also includes end cover one, which is fixedly installed on the top of the column tube, and an exhaust hole is provided on the end cover one, and the exhaust hole is connected to the column tube. The output end of the linear drive two is slidably arranged with the end cover one, and a groove is provided in the output end of the linear drive two. The mobile phase delivery tube is located in the groove, and the end of the mobile phase delivery tube away from the cavity is fixedly connected to a pump.

[0014] In a preferred embodiment, the extrusion mechanism further includes a linear drive 1, which is fixedly arranged on the support frame, a fixed seat is fixedly arranged on the output end of the linear drive 1, and the linear drive 2 is fixedly installed on the fixed seat.

[0015] In a preferred embodiment, a rotary driver is fixedly provided on the piston, the output end of the rotary driver is fixedly provided with the center point of the rotating disk, a connecting hole is opened on the piston, a sealing ring is fixedly provided on the inner wall of the connecting hole, and the output end of the rotary driver is rotatably provided in the sealing ring.

[0016] In a preferred embodiment, a drain port is provided on the second end cap, and the drain port is located at the center of the second end cap, and is used to drain the liquid in the column tube.

[0017] In a preferred embodiment, a second sealing ring is provided on the outer fixed sleeve of the piston, and the second sealing ring is in close contact with the inner wall of the column tube.

[0018] The beneficial effects of the present invention are:

[0019] 1. The present invention sets a rotating disk in the piston, and the cavity is in a closed state with the outside. The bottom of the piston can apply stable pressure to the stationary phase, ensuring that the stationary phase can be evenly and tightly filled in the column tube. The rotating disk is driven to rotate, and the through hole 2 is connected with the through hole 1. The mobile phase liquid is discharged onto the stationary phase through multiple through holes 1 evenly arranged at equal intervals. The multiple dispersed through holes 1 can distribute the mobile phase more evenly, reduce the impact on the stationary phase, and enable the mobile phase to fully participate in the separation process. Different components can be well separated, thereby improving the separation efficiency.

[0020] 2. The present invention provides a material removal mechanism and drives the rotating disk to rotate, thereby raising the height of the movable shaft to prevent filler particles from entering the cavity. The filler particles will not cause wear to the movable shaft or the rotating disk, thereby ensuring the column efficiency of the chromatographic column and further improving the separation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0022] Figure 2 For the present invention Figure 1 Schematic diagram of the structure of the main view.

[0023] Figure 3 It is a schematic diagram of the three-dimensional structure of the support frame of the present invention.

[0024] Figure 4 It is a schematic diagram of the cross-sectional structure of the column tube of the present invention from the front view.

[0025] Figure 5 This is a schematic diagram of the cross-sectional structure of the piston of the present invention from the front view.

[0026] Figure 6 For the present invention Figure 5 Schematic diagram of the movement trajectory of the middle piston when viewed from above.

[0027] Figure 7 It is a schematic cross-sectional structural diagram of the movable shaft of the present invention from the main view.

[0028] Figure 8 For the present invention Figure 7 Schematic diagram of the moving trajectory of the movable axis.

[0029] Figure 9 For the present invention Figure 8 Schematic diagram of the structure of part A.

[0030] Figure 10 It is a schematic diagram of the three-dimensional structure of the rotating disk of the present invention.

[0031] Figure 11 For the present invention Figure 10 Schematic diagram of the three-dimensional structure in which the central movable shaft is located in the arc hole.

[0032] The accompanying drawings are marked as follows: 1. support frame; 11. support plate; 12. fixing ring; 2. chromatographic separation component; 21. column tube; 211. connecting ring; 22. end cover one; 23. end cover two; 24. filter screen; 3. extrusion mechanism; 31. linear drive one; 32. fixing seat; 33. linear drive two; 4. column pressure maintaining mechanism; 41. piston; 411. cavity; 412. through hole one; 42. rotating disk; 421. through hole two; 43. mobile phase delivery tube; 44. rotating drive; 5. material removal mechanism; 51. movable shaft; 511. connecting seat; 52. arc hole; 521. guide groove; 522. through hole five; 53. through hole three; 54. through hole four. DETAILED DESCRIPTION

[0033] The present application is described in further detail below in conjunction with the accompanying drawings. It is necessary to point out that the following specific implementation methods are only used to further illustrate the present application and cannot be understood as limiting the scope of protection of the present application. Technicians in this field can make some non-essential improvements and adjustments to the present application based on the above application content.

[0034] Refer to the instruction manual Figures 1 to 6 A liquid chromatography column chromatography separation device includes a support frame 1, a chromatography separation component 2 is provided on the support frame 1, the chromatography separation component 2 includes a column tube 21, the bottom end of the column tube 21 is provided with an end cap 23, the top of the end cap 23 is provided with a filter screen 24, the stationary phase is poured into the column tube 21 through the top end of the column tube 21, and the filter screen 24 is used to filter the stationary phase;

[0035] The support frame 1 is provided with an extrusion mechanism 3, which includes a second linear drive 33. A column pressure maintaining mechanism 4 is provided at the output end of the second linear drive 33. The column pressure maintaining mechanism 4 includes a piston 41. A cavity 411 is defined within the piston 41. A first through-hole 412 is defined at the bottom of the piston 41. A rotating disk 42 is rotatably provided on the bottom inner wall of the cavity 411. A second through-hole 421 is defined on the rotating disk 42. A mobile phase delivery pipe 43 is fixedly connected to the cavity 411. The mobile phase delivery pipe 43 is used to deliver the mobile phase into the cavity 411.

[0036] The output end of the linear driver 2 33 is used to drive the piston 41 to move along the length direction of the column tube 21 in the column tube 21. When the through hole 1 412 and the through hole 2 421 are misaligned, the piston 41 applies a constant pressure on the stationary phase by moving vertically downward in the column tube 21. When the through hole 1 412 and the through hole 2 421 are connected, the mobile phase in the cavity 411 enters the stationary phase to achieve separation.

[0037] It should be noted that, referring to Figure 6 The bottom of the piston 41 is provided with a plurality of through-holes 1 412 , which are evenly spaced at the bottom of the piston 41 . The number of through-holes 2 421 corresponds to the number of through-holes 1 412 . An auxiliary hole is provided on the cavity 411 , and a hole cover is threadedly connected to the auxiliary hole. When the mobile phase liquid is injected into the cavity 411 , the auxiliary hole is used to discharge the gas in the cavity 411 , and the hole cover is used to block the auxiliary hole. The end of the mobile phase delivery tube 43 away from the cavity 411 is fixedly connected to a pump. The pump is used to eject the mobile phase from the mobile phase delivery tube 43 and apply pressure to the ejected mobile phase, so that the mobile phase can stably pass through the stationary phase even under the influence of the resistance of the stationary phase particles. The stationary phase includes but is not limited to matrix materials, such as silica gel, polymer microspheres; chemically bonded phases, such as alkyl silanes, aromatic silanes; ion exchange groups, such as sulfonic acid groups, quaternary ammonium groups and other components. When the stationary phase is loaded into the chromatographic column, the stationary phase is first dissolved in a solvent such as methanol or acetonitrile to form a suspension, the suspension is fully mixed, and then the suspension is poured into the column tube 21. The suspension is the initial state of the stationary phase. When the liquid in the suspension is discharged from the column tube 21, the remaining particle filler in the column tube 21 is the stationary phase. The mobile phase includes but is not limited to organic solutions, buffered hydrochloric acid solutions, water and other substances. Based on the difference in distribution coefficients and different physicochemical properties between the stationary phase and the mobile phase, the mobile phase moves at different speeds in the chromatographic column, thereby achieving separation. The mobile phase finally passes through the stationary phase to generate an effluent. After the effluent is discharged from the column tube 21, an ultraviolet detector or a mass spectrometer is used to detect the absorption characteristics of the effluent to ultraviolet light and the molecular weight and structure of the effluent substance to achieve qualitative and quantitative analysis of the effluent. The principle of separation achieved by the mobile phase passing through the stationary phase is a mature existing technology and will not be elaborated on here.

[0038] It should also be noted that the chromatographic separation component 2 also includes an end cap 22, which is fixedly mounted on the top of the column tube 21. An exhaust hole is provided on the end cap 22, which is in communication with the column tube 21. The output end of the linear actuator 33 is slidably disposed with the end cap 22. A groove is provided within the output end of the linear actuator 33, and the mobile phase delivery tube 43 is located within the groove. A drain port is provided on the end cap 23, located at the center of the end cap 23, and is used to drain the liquid from the column tube 21. The end of the mobile phase delivery tube 43 away from the piston 41 is configured as a hose to facilitate adjustment of the bottom end movement of the mobile phase delivery tube 43 according to the movement of the output end of the linear actuator 33.

[0039] Furthermore, the design of the exhaust hole can prevent the piston 41 from generating negative pressure in the upper part of the column tube 21 during the movement. A guide tube is fixedly installed in the discharge port. The end cover 1 22 and the end cover 2 23 are both included but not limited to being fixed on the column tube 21 by bolts. A filter screen plate 24 is fixedly provided on the top of the end cover 23. The size of the mesh of the filter screen plate 24 is set according to the use requirements. As a mature existing technology, it will not be elaborated here.

[0040] The specific implementation scenario is as follows: pour the mobile phase suspension into the column tube 21, and rinse the buffered hydrochloric acid solution on the inner wall of the column tube 21 to avoid residual suspension on the inner wall of the column tube 21, inject the mobile phase test solution into the cavity 411, and then start the linear drive 2 33. The output shaft of the linear drive 2 33 drives the piston 41 to move vertically into the column tube 21. At this time, the through hole 1 412 and the through hole 2 421 are in a misaligned state, that is, the cavity 411 is not connected to the outside. Then the end cover 1 22 is installed on the top of the column tube 21. At this time, the suspension is located between the bottom of the piston 41 in the column tube 21 and the filter screen plate 24, so that the piston 41 continues to move downward in the column tube 21. The downward movement of the piston 41 will generate pressure on the suspension between the piston 41 and the filter screen plate 24, and the excess liquid in the suspension will After being filtered through the filter screen plate 24 and discharged through the drain port, when the excess liquid in the suspension is discharged, the remaining particle filler is the stationary phase. The piston 41 squeezes the stationary phase to ensure that the stationary phase is evenly and tightly filled in the column tube 21 to prevent it from moving or deforming when the mobile phase passes through, and the piston 41 always applies constant pressure to the stationary phase. Then the pump is started, the pump conveys the mobile phase into the cavity 411 and drives the rotating disk 42 to rotate. The rotation of the rotating disk 42 connects the through hole 2 421 with the through hole 1 412, and the mobile phase liquid in the cavity 411 is discharged onto the stationary phase through the through hole 1 412. Since there are multiple through holes 1 412, the mobile phase is discharged to the surface of the stationary phase through multiple different through holes 1 412, and the mobile phase is subjected to chromatographic separation when passing through the stationary phase.

[0041] In the prior art, a mobile phase delivery tube 43 is provided at the bottom of the piston 41. There are two ways to drive the piston 41 to compress the stationary phase in the column tube 21. The first way is that when the piston 41 is driven to compress the column tube 21, in order to prevent the liquid in the stationary phase from entering the bottom end of the mobile phase delivery tube 43, a pump is required to continuously inject gas into the piston 41 to apply pressure, so as to ensure that the pressure generated on the bottom end of the column tube 21 when the piston 41 moves downward in the column tube 21 is balanced. When the stationary phase is compacted, the pump again delivers the mobile phase to allow the mobile phase to enter the column tube 21. The second way is to install a gate valve at the bottom end of the mobile phase delivery tube 43. When the piston 41 moves downward in the column tube 21 to apply pressure to the stationary phase, the gate valve is in a closed state. When the stationary phase is compacted, the gate valve is opened again to allow the mobile phase to enter the column tube 21. However, the solution in method one may have the risk of gas mixing into the stationary phase, thereby destroying the structure of the stationary phase and affecting the separation effect. The solution in method two may cause some particles in the stationary phase to enter the pipeline and contact the valve core of the gate valve. When the valve core rotates, the presence of fine particles in the stationary phase causes the valve core to be worn and eventually causes the gate valve to leak. Both implementation plans in the prior art have disadvantages.

[0042] Compared with the prior art, in this solution, when the piston 41 moves downward in the column tube 21 and applies pressure to the suspension, the through hole 1 412 and the through hole 2 421 are misaligned, that is, the cavity 411 is in a closed state with the outside, and the bottom of the piston 41 can apply stable pressure to the stationary phase to ensure that the stationary phase can be evenly and tightly filled in the column tube 21. When the stationary phase is compacted by the constant pressure applied by the piston 41, the rotating disk 42 is driven to rotate, and the through hole 2 421 is connected with the through hole 1 412. The mobile phase liquid is discharged onto the stationary phase through multiple evenly spaced through holes 1 412. Especially when the diameter of the column tube 21 is large, the multiple dispersed through holes 1 412 can distribute the mobile phase more evenly, reduce the impact on the stationary phase, and enable the mobile phase to fully participate in the separation process, so that different components can be well separated, thereby improving the separation efficiency.

[0043] Refer to the instruction manual Figures 7 to 11Since the piston 41 is provided with a cavity 411 for storing the mobile phase, in order to make the bottom of the piston 41 have greater strength and avoid deformation when the bottom of the piston 41 applies pressure to the stationary phase, the bottom of the piston 41 and the bottom inner wall of the cavity 411 need to have a greater wall thickness to improve the overall strength of the piston 41, that is, the through hole 1 412 needs to have a greater length, especially in the application of high-pressure liquid chromatography columns. Preferably, for example, the length of the through hole 1 412 is set to 2 to 3 cm. When the length of the through hole 1 412 is large, the bottom of the piston 41 applies a downward squeezing force on the stationary phase, which will cause some of the filler particles in the stationary phase to stick to the inner wall of the through hole 1 412 and contact the bottom of the rotating disk 42. When the through hole 1 412 needs to be connected to the through hole 2 421, the filler particles attached to the bottom of the through hole 1 412 will rotate with the bottom of the rotating disk 42, resulting in the rotation. The bottom of the disk 42 causes wear, and the wear of the bottom of the rotating disk 42 will cause the overall sealing performance of the rotating disk 42 to be damaged and produce scratches, and the wear of the bottom of the rotating disk 42 will also cause the mobile phase to enter the scratches and cause mobile phase residue, which is not conducive to subsequent cleaning. Since the diameter of the column tube 21 is large, the number of through holes 412 opened at the bottom of the piston 41 is large, and some filler particles in the stationary phase will also enter the cavity 411. The reduction of filler particles in the stationary phase will lead to a decrease in column efficiency. In order to avoid the above situation, a material removal mechanism 5 is specifically provided in the piston 41. The material removal mechanism 5 includes a movable shaft 51, an arc-shaped hole 52 is opened on the rotating disk 42, and the movable shaft 51 is slidably set in the arc-shaped hole 52 and the through hole 412. A guide groove 521 is opened on the inner wall of the arc-shaped hole 52, and a connecting seat 511 is fixedly set on the movable shaft 51, and the connecting seat 511 is slidably set in the guide groove 521. A fourth through-hole 54 is defined in the rotating disk 42, and a third through-hole 53 is defined on the bottom inner wall of the cavity 411. The third through-hole 53 mates with the fourth through-hole 54. The bottom end of the third through-hole 53 communicates with the first through-hole 412, which also mates with the bottom end of the movable shaft 51. A fifth through-hole 522 is defined in the arcuate hole 52, communicating with the fourth through-hole 54. The fifth through-hole 522 is used to transfer the mobile phase within the arcuate hole 52 to the fourth through-hole 54.

[0044] It should be noted that the aperture of through hole three 53 in the figure is only a schematic diagram and does not refer to the difference in diameter between through hole three 53 and through hole one 412. The aperture of through hole three 53 can be increased to meet usage requirements. As a mature existing technology, it will not be elaborated on here.

[0045] It should also be noted that the arcuate hole 52 is concentric with the rotating disk 42, and the depth of the guide groove 521 on the inner wall of the arcuate hole 52 is concentric with the arcuate hole 52. That is, when the rotating disk 42 rotates, the spacing between the connecting seat 511 and the guide groove 521 remains the same. The provision of the through hole 522 prevents residual mobile phase from remaining in the arcuate hole 52.

[0046] In this embodiment, when the piston 41 moves vertically downward in the column tube 21 to apply pressure to the stationary phase, the bottom end of the movable shaft 51 is flush with the bottom of the piston 41, and the particle filler in the stationary phase contacts the bottom of the piston 41 and the bottom end of the movable shaft 51. When the stationary phase is compacted, when it is necessary to introduce the mobile phase in the cavity 411 into the column tube 21, the rotating disk 42 is driven to rotate. When the rotating disk 42 rotates, the movable shaft 51 can be vertically moved upward in the through hole 1 412 under the guidance of the guide groove 521. At this time, there may be a little particle filler adhering to the bottom end of the movable shaft 51. Figure 8 and Figure 9 As the movable shaft 51 continues to move upward, in the vertical direction, the through hole four 54 gradually approaches the corresponding through hole three 53. When the bottom end of the movable shaft 51 and the bottom end of the through hole three 53 are at the same horizontal height, or when the movable shaft 51 moves upward to connect the through hole one 412 with the bottom end of the through hole three 53, the through hole four 54 and the through hole three 53 are also connected. The mobile phase in the cavity 411 can flow into the stationary phase through the cooperation of the through hole four 54, the through hole three 53 and the through hole one 412, and the mobile phase discharged from the bottom end of the through hole three 53 can flush the filler particles adhered to the bottom end of the movable shaft 51, preventing the filler particles from entering the cavity 411. The filler particles will not cause wear to the movable shaft 51 or the rotating disk 42, thereby ensuring the column efficiency of the chromatographic column and improving the separation efficiency. After the rotating disk 42 rotates in the opposite direction, the movable shaft 51 is reset.

[0047] Refer to the instruction manual Figures 1 to 3 In order to facilitate the removal of the lower column tube 21 and the cleaning of the inner wall of the column tube 21, specifically, two support plates 11 are fixedly provided on the support frame 1, and a mounting hole is opened on the support plate 11. An auxiliary ring is fixedly provided on the support plate 11, and the auxiliary ring is concentrically arranged with the mounting hole. A fixing ring 12 is placed in each of the two auxiliary rings, and the diameter of the fixing ring 12 is larger than the aperture of the mounting hole. Two connecting rings 211 are fixedly provided on the column tube 21, and the fixing ring 12 is fixed to the corresponding connecting ring 211 by bolts.

[0048] It should be noted that the fixing ring 12 includes two semicircular rings, which can form a complete fixing ring 12, that is, the fixing ring 12 is a complete circular ring composed of two semicircular rings, and the diameter of the connecting ring 211 is smaller than the aperture of the mounting hole.

[0049] It should also be noted that when the column tube 21 needs to be installed on the support frame 1, the column tube 21 is first placed in the mounting hole, and then the fixing ring 12 is placed in the mounting hole, and then the fixing ring 12 is placed in the auxiliary ring, and the fixing ring 12 is fixed to the connecting ring 211 by bolts. When the fixing ring 12 and the connecting ring 211 are fixed, the column tube 21 is fixed to the support frame 1.

[0050] Refer to the instruction manual Figures 1 to 4 In order to facilitate the subsequent disassembly of the column tube 21, specifically, the extrusion mechanism 3 also includes a linear drive 1 31, which is fixedly arranged on the support frame 1, and a fixed seat 32 is fixedly arranged on the output end of the linear drive 1 31, and a linear drive 2 33 is fixedly installed on the fixed seat 32.

[0051] It should be noted that the linear actuator 1 31 is configured as a hydraulic cylinder, which is disposed on the support plate 11, and the fixing seat 32 is fixedly disposed on the output end of the hydraulic cylinder. The fixing seat 32 is also configured as a hydraulic cylinder, and the piston 41 is fixedly disposed on the output end of the hydraulic cylinder.

[0052] It should also be noted that when the linear driver 1 31 is started, the mobile drive fixed seat 32 at the output end of the linear driver 1 31 moves vertically. The movement of the fixed seat 32 can adjust the height of the linear driver 2 33. When removing the column tube 21, raising the height of the fixed seat 32 can facilitate the removal of the lower column tube 21. When the linear driver 2 33 is started, the mobile drive piston 41 at the output end of the linear driver 2 33 moves in the column tube 21 along the length direction of the column tube 21.

[0053] Refer to the instruction manual Figures 4 to 8 To facilitate the rotation of rotating disk 42, thereby sealing or releasing the blockage at the bottom of piston 41, a rotary actuator 44 is fixedly mounted on piston 41. The output end of rotary actuator 44 is fixedly positioned at the center of rotating disk 42. A connecting hole is formed in piston 41, and a first sealing ring is fixedly mounted on the inner wall of the connecting hole. The output end of rotary actuator 44 is rotatably mounted within the sealing ring. A second sealing ring is fixedly mounted on the outer side of piston 41 and tightly fits against the inner wall of column tube 21.

[0054] It should be noted that the rotation driver 44 is configured as a motor, the motor is fixedly mounted on the piston 41 , and the output shaft of the motor is fixedly mounted on the rotating disk 42 .

[0055] It should also be noted that when the rotary driver 44 is started, the output end of the rotary driver 44 rotates to drive the rotary disk 42 to rotate, and the rotary driver 44 can also apply pressure to the rotary disk 42 so that the rotary disk 42 always fits against the bottom inner wall of the cavity 411.

[0056] The above embodiments merely illustrate several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the scope of the present invention, all of which fall within the scope of protection of the present invention.

Claims

1. A liquid chromatography column chromatography separation device, characterized in that, The invention comprises a support frame (1), wherein a chromatographic separation component (2) is provided on the support frame (1), wherein the chromatographic separation component (2) comprises a column tube (21), wherein a second end cap (23) is provided at the bottom end of the column tube (21), and a filter screen (24) is provided at the top end of the second end cap (23), wherein a stationary phase is poured into the column tube (21) through the top end of the column tube (21), and the filter screen (24) is used for filtering the stationary phase; The support frame (1) is provided with an extrusion mechanism (3), the extrusion mechanism (3) includes a second linear driver (33), the output end of the second linear driver (33) is provided with a column pressure maintaining mechanism (4), the column pressure maintaining mechanism (4) includes a piston (41), a cavity (411) is provided inside the piston (41), a through hole (412) is provided at the bottom of the piston (41), a rotating disk (42) is rotatably provided on the inner wall of the bottom of the cavity (411), a through hole (421) is provided on the rotating disk (42), a mobile phase delivery pipe (43) is fixedly connected in the cavity (411), and the mobile phase delivery pipe (43) is used to deliver the mobile phase into the cavity (411); The output end of the second linear driver (33) is used to drive the piston (41) to move in the column tube (21) along the length direction of the column tube (21). When the through hole (412) and the through hole (421) are misaligned, the piston (41) applies a constant pressure to the stationary phase by moving vertically downward in the column tube (21). When the through hole (412) and the through hole (421) are connected, the mobile phase in the cavity (411) enters the stationary phase to achieve separation. A material removal mechanism (5) is provided in the piston (41), and the material removal mechanism (5) includes a movable shaft (51). An arc-shaped hole (52) is provided on the rotating disk (42). The movable shaft (51) is slidably provided in the arc-shaped hole (52) and the through hole (412). A guide groove (521) is provided on the inner wall of the arc-shaped hole (52). A connecting seat (511) is fixedly provided on the movable shaft (51), and the connecting seat (511) is slidably provided in the guide groove (521). A through hole four (54) is provided on the rotating disk (42), and a through hole three (53) is provided on the bottom inner wall of the cavity (411). The through hole three (53) is adapted to the through hole four (54), and the bottom end of the through hole three (53) is connected to the through hole one (412). The bottom end of the through hole three (53) is also adapted to the bottom end of the movable shaft (51); A through hole five (522) is provided on the arc-shaped hole (52), and the through hole five (522) is connected to the through hole four (54). The through hole five (522) is used to transport the mobile phase in the arc-shaped hole (52) to the through hole four (54).

2. A liquid chromatography column chromatography separation device according to claim 1, characterized in that: Two support plates (11) are fixedly provided on the support frame (1), a mounting hole is provided on the support plate (11), an auxiliary ring is fixedly provided on the support plate (11), the auxiliary ring is concentrically arranged with the mounting hole, a fixing ring (12) is placed in each of the two auxiliary rings, the diameter of the fixing ring (12) is larger than the aperture of the mounting hole, and two connecting rings (211) are fixedly provided on the column tube (21), and the fixing ring (12) and the corresponding connecting ring (211) are fixed by bolts.

3. A liquid chromatography column chromatography separation device according to claim 2, characterized in that: The chromatographic separation component (2) further includes an end cap (22), which is fixedly mounted on the top of the column tube (21), and an exhaust hole is provided on the end cap (22), which is communicated with the column tube (21). The output end of the linear driver (33) is slidably arranged with the end cap (22), and a groove is provided in the output end of the linear driver (33), and the mobile phase delivery tube (43) is located in the groove. The end of the mobile phase delivery tube (43) away from the cavity (411) is fixedly connected to a pump.

4. A liquid chromatography column chromatography separation device according to claim 3, characterized in that: The extrusion mechanism (3) further comprises a linear drive 1 (31), wherein the linear drive 1 (31) is fixedly arranged on the support frame (1), a fixed seat (32) is fixedly arranged on the output end of the linear drive 1 (31), and the linear drive 2 (33) is fixedly mounted on the fixed seat (32).

5. The liquid chromatography column chromatography separation device according to claim 4, characterized in that: A rotation driver (44) is fixedly provided on the piston (41), and an output end of the rotation driver (44) is fixedly provided with a center point of the rotating disk (42). A connecting hole is provided on the piston (41), and a sealing ring 1 is fixedly provided on the inner wall of the connecting hole. The output end of the rotation driver (44) is rotatably provided in the sealing ring.

6. The liquid chromatography column chromatography separation device according to claim 5, characterized in that: The second end cap (23) is provided with a liquid drain port, which is located at the center of the second end cap (23) and is used to drain the liquid in the column tube (21).

7. The liquid chromatography column chromatography separation device according to claim 6, characterized in that: The outer fixed sleeve of the piston (41) is provided with a second sealing ring, and the second sealing ring is tightly fitted to the inner wall of the column tube (21).

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