Split type test strip for photochemical analysis and preparation method thereof

Abstract

The invention provides a photochemical analysis test strip and a preparation method thereof. The preparation method comprises the following steps: S1, manufacturing a liquid absorption tank and a reagent storage tank on the bottom surface of a reagent runner module; s2, arranging a reagent runner module on a bottom plate to define a liquid suction port and a reagent storage area; s3, sucking the liquid reagent into a reagent storage area through a liquid suction port, and starting a freeze-drying treatment process to form a freeze-dried powder reagent in the reagent storage area; s4, making a communication groove and a test groove in the bottom surface of the test flow channel module; and S5, arranging the test flow channel module on the bottom plate, and aligning and connecting the test flow channel module and the reagent flow channel module. The reagent runner module is mounted on the bottom plate firstly, and then a liquid reagent is sucked into the reagent storage area to be subjected to freeze-drying treatment, so that a freeze-dried powder reagent can be formed in the reagent storage area, and the preparation process is simplified; and before the freeze-dried powder reagent is formed, the test runner module does not need to be mounted on the bottom plate, so that the suction resistance of the liquid reagent is reduced, and the suction process of the liquid reagent is smoother.

Description

Technical Field

[0001] The present invention relates to the technical field of body fluid analysis, and particularly to a split test strip for photochemical analysis and a preparation method thereof. Background Art

[0002] A photochemical analysis test strip is a rapid diagnostic tool based on an optical detection principle. By reacting a specific chemical reagent loaded on the test strip with a target substance, color change, fluorescence or chemiluminescence signals are generated, and detection and analysis are realized in combination with an optical detection instrument (such as a spectrophotometer, a fluorescence detector).

[0003] For example, the Chinese utility model patent with the publication number CN221612871U discloses a hemoglobin determination test paper, which includes a hydrophilic membrane, a liquid guide plate, a reaction membrane, and a support plate stacked in sequence from top to bottom. The hydrophilic membrane is provided with a first ventilation hole, the liquid guide plate is provided with a guide groove and a reaction groove communicated with the guide groove, the support plate is provided with a sampling groove and a detection groove, the sampling groove is correspondingly communicated with the guide groove, the top surface of the reaction membrane is correspondingly arranged with the reaction groove, and the bottom surface of the reaction membrane is correspondingly arranged with the detection groove. In the preparation process of the test paper of the above prior art, it is necessary to separately prepare the reaction membrane and then arrange the prepared reaction membrane between the reaction groove and the detection groove, and the preparation process is relatively complicated.

[0004] Therefore, the prior art still needs to be improved and developed. Summary of the Invention

[0005] The purpose of the present invention is to provide a split test strip for photochemical analysis and a preparation method thereof in view of the defects and deficiencies of the prior art.

[0006] In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] The present invention provides a preparation method for a split test strip for photochemical analysis, including the following steps:

[0008] S1. Make a liquid absorption groove and a reagent storage groove that are sequentially communicated on the bottom surface of the reagent flow channel module;

[0009] S2. Place the reagent flow channel module of step S1 on the bottom plate, so that the liquid absorption groove and the bottom plate enclose a liquid absorption port, and the reagent storage groove and the bottom plate enclose a reagent storage area;

[0010] S3. Inhale a liquid reagent through the liquid absorption port. After the liquid reagent fills the reagent storage area, start the freeze-drying treatment process to form a freeze-dried powder reagent in the reagent storage area;

[0011] S4. Make a communication groove and a test groove that are sequentially communicated on the bottom surface of the test flow channel module;

[0012] S5. Place the test flow channel module in step S4 on the bottom plate, such that the communication groove and the bottom plate enclose a test flow channel, and the test groove and the bottom plate enclose a test area; align and connect the test flow channel module with the reagent flow channel module to achieve communication between the test flow channel and the reagent storage area.

[0013] In the present invention, by first installing the reagent flow channel module on the bottom plate, and then sucking the liquid reagent into the reagent storage area through the liquid suction port and performing freeze-drying treatment, a freeze-dried powder reagent can be formed in the reagent storage area, which is beneficial to simplifying the preparation process; moreover, before the formation of the freeze-dried powder reagent, there is no need to install the test flow channel module on the bottom plate, reducing the resistance during the suction of the liquid reagent and making the suction process of the liquid reagent smoother.

[0014] According to the above solution, step S1 specifically includes the following steps:

[0015] S11. Cut first openings corresponding to the liquid suction groove and the reagent storage groove on the first double-sided adhesive layer;

[0016] S12. Paste the first double-sided adhesive layer cut in step S11 on the bottom surface of the first hydrophilic cover plate.

[0017] The reagent flow channel module is composed of the first double-sided adhesive layer and the first hydrophilic cover plate. By cutting the first openings on the first double-sided adhesive layer and then pasting the first double-sided adhesive layer on the first hydrophilic cover plate, the liquid suction groove and the reagent storage groove can be formed on the bottom surface of the reagent flow channel module, which is beneficial to simplifying the preparation process of the reagent flow channel module.

[0018] According to the above solution, the first hydrophilic cover plate is set as a first hydrophilic transparent plate; the first double-sided adhesive layer is set as a first opaque layer.

[0019] According to the above solution, the bottom plate in step S2 is set as a hydrophobic plate.

[0020] According to the above solution, step S4 specifically includes the following steps:

[0021] S41. Cut second openings corresponding to the communication groove and the test groove on the second double-sided adhesive layer;

[0022] S42. Paste the second double-sided adhesive layer cut in step S41 on the bottom surface of the second hydrophilic cover plate.

[0023] The test flow channel module is composed of the second double-sided adhesive layer and the second hydrophilic cover plate. By cutting the second openings on the second double-sided adhesive layer and then pasting the second double-sided adhesive layer on the second hydrophilic cover plate, the communication groove and the test groove can be formed on the bottom surface of the test flow channel module, which is beneficial to simplifying the preparation process of the test flow channel module.

[0024] According to the above solution, the second hydrophilic cover plate is set as a second hydrophilic transparent plate; the second double-sided adhesive layer is set as a second opaque layer.

[0025] According to the above solution, step S4 further includes opening air holes communicating with the test groove on the test flow channel module. The gas in the test flow channel module is discharged to the outside through the air holes, so that the blood sample can smoothly flow into the test area.

[0026] According to the above solution, step S4 further includes making air-permeable grooves communicating with the test groove on the bottom surface of the test flow channel module; the air-permeable grooves are used to enclose an air-permeable area with the bottom plate.

[0027] According to the above solution, the area of the air-permeable area is smaller than the area of the test area. By setting the air-permeable area, it is convenient for exhaust, and users can judge whether the test area is filled with blood samples by observing the blood samples entering the air-permeable area.

[0028] The present invention also provides a split test strip prepared by the above preparation method.

[0029] The beneficial effects of the present invention are as follows:

[0030] 1. By first installing the reagent flow channel module on the bottom plate, and then sucking the liquid reagent into the reagent storage area through the liquid suction port and then performing freeze-drying treatment, a freeze-dried powder reagent can be formed in the reagent storage area, which is beneficial to simplifying the preparation process.

[0031] 2. Before the formation of the freeze-dried powder reagent, the test flow channel module does not need to be installed on the bottom plate, reducing the resistance to the suction of the liquid reagent and making the process of sucking the liquid reagent smoother.

[0032] 3. Forming the freeze-dried powder reagent in the reagent flow channel module enables the blood sample to be first sucked into the reagent storage area to dissolve the freeze-dried powder reagent, and then enter the test flow channel module, so that the test flow channel module will not form resistance to the blood sample, which is beneficial to improving the sample injection speed of the blood sample in the test flow channel module. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 is a schematic structural diagram of the split test strip of the present invention;

[0034] Figure 2 is a schematic bottom surface structure diagram of the reagent flow channel module of the present invention;

[0035] Figure 3 is an exploded structural diagram of the reagent flow channel module of the present invention;

[0036] Figure 4 is a schematic structural diagram of the reagent flow channel module provided on the bottom plate of the present invention; Figure 3 The dotted line in represents the invisible line in the reagent flow channel module;

[0037] Figure 5 It is a schematic view of the bottom surface structure of the test flow channel module of the present invention;

[0038] Figure 6 It is a schematic exploded view of the test flow channel module of the present invention;

[0039] Figure 7 It is a schematic view of the reagent flow channel module and the test flow channel module provided on the bottom plate of the present invention; Figure 7 The dotted lines in the figure represent the invisible lines in the reagent flow channel module and the test flow channel module.

[0040] In the figure: 1. Reagent flow channel module; 11. Liquid absorption groove; 12. Reagent storage groove; 13. First double-sided adhesive layer; 131. First opening; 14. First hydrophilic cover plate; 2. Bottom plate; 21. Liquid absorption port; 22. Reagent storage area; 23. Test flow channel; 24. Test area; 25. Ventilation area; 3. Test flow channel module; 31. Communication groove; 32. Test groove; 33. Second double-sided adhesive layer; 331. Second opening; 34. Second hydrophilic cover plate; 35. Air hole; 36. Ventilation groove. Specific embodiments

[0041] The technical solutions of the present invention will be described below in conjunction with the accompanying drawings and embodiments.

[0042] The present invention provides a preparation method for a split test strip for photochemical analysis, including the following steps:

[0043] S1. As shown in Figure 2 a liquid absorption groove 11 and a reagent storage groove 12 that are sequentially communicated are made on the bottom surface of the reagent flow channel module 1;

[0044] S2. As shown in Figure 3 the reagent flow channel module 1 obtained in step S1 is placed on the bottom plate 2, so that the liquid absorption groove 11 and the bottom plate 2 enclose a liquid absorption port 21, and the reagent storage groove 12 and the bottom plate 2 enclose a reagent storage area 22;

[0045] S3. A liquid reagent is inhaled through the liquid absorption port 21, and after the liquid reagent fills the reagent storage area 22, a freeze-drying treatment process is started so that a freeze-dried powder reagent is formed in the reagent storage area 22;

[0046] S4. As shown in Figure 5 a communication groove 31 and a test groove 32 that are sequentially communicated are made on the bottom surface of the test flow channel module 3;

[0047] S5. As shown in Figure 7As shown in the figure, the test flow channel module 3 of step S4 is disposed on the bottom plate 2, such that the communication groove 31 and the bottom plate 2 enclose a test flow channel 23, and the test groove 32 and the bottom plate 2 enclose a test area 24; the test flow channel module 3 is aligned and connected with the reagent flow channel module 1 to enable the test flow channel 23 to communicate with the reagent storage area 22.

[0048] In the present invention, the reagent flow channel module 1 is first installed on the bottom plate 2, and then the liquid reagent is sucked into the reagent storage area 22 through the liquid suction port 21. The whole bottom plate 2 is freeze-dried, and freeze-dried powder reagent can be formed in the reagent storage area 22, which is beneficial to simplifying the preparation process; moreover, before the formation of the freeze-dried powder reagent, the test flow channel module 3 does not need to be installed on the bottom plate 2, reducing the resistance of the liquid reagent suction and making the liquid reagent suction process smoother.

[0049] Further, as Figures 1-3 shown, step S1 specifically includes the following steps:

[0050] S11. Cut a first opening 131 corresponding to the liquid suction groove 11 and the reagent storage groove 12 on the first double-sided adhesive layer 13;

[0051] S12. Paste the first double-sided adhesive layer 13 cut in step S11 on the bottom surface of the first hydrophilic cover plate 14.

[0052] The reagent flow channel module 1 is composed of the first double-sided adhesive layer 13 and the first hydrophilic cover plate 14. By cutting the first opening 131 on the first double-sided adhesive layer 13 and then pasting the first double-sided adhesive layer 13 on the first hydrophilic cover plate 14, the liquid suction groove 11 and the reagent storage groove 12 can be made on the bottom surface of the reagent flow channel module 1, which is beneficial to simplifying the preparation process of the reagent flow channel module 1.

[0053] Further, the first hydrophilic cover plate 14 is set as a first hydrophilic transparent plate; the first double-sided adhesive layer 13 is set as a first opaque layer.

[0054] Further, the bottom plate 2 in step S2 is set as a hydrophobic plate.

[0055] Further, as Figures 5-6 shown, step S4 specifically includes the following steps:

[0056] S41. Cut a second opening 331 corresponding to the communication groove 31 and the test groove 32 on the second double-sided adhesive layer 33;

[0057] S42. Paste the second double-sided adhesive layer 33 cut in step S41 on the bottom surface of the second hydrophilic cover plate 34.

[0058] The test flow channel module 3 is composed of a second double-sided adhesive layer 33 and a second hydrophilic cover plate 34. By cutting a second opening 331 in the second double-sided adhesive layer 33 and then pasting the second double-sided adhesive layer 33 on the second hydrophilic cover plate 34, a communication groove 31 and a test groove 32 can be formed on the bottom surface of the test flow channel module 3, which is beneficial to simplifying the preparation process of the test flow channel module 3.

[0059] Furthermore, the second hydrophilic cover plate 34 is set as a second hydrophilic transparent plate; the second double-sided adhesive layer 33 is set as a second opaque layer.

[0060] Furthermore, as Figures 5-7 shown, the step S4 further includes opening an air hole 35 communicating with the test groove 32 on the test flow channel module 3. The gas in the test flow channel module 3 is discharged to the outside through the air hole 35, so that the blood sample can smoothly flow into the test area 24.

[0061] Furthermore, as Figure 5 、 7 shown, the step S4 further includes making a breathable groove 36 communicating with the test groove 32 on the bottom surface of the test flow channel module 3; the breathable groove 36 is used to form a breathable area 25 with the bottom plate 2.

[0062] Furthermore, the area of the breathable area 25 is smaller than the area of the test area 24. By setting the breathable area 25, it is convenient for exhaust, and users can judge whether the test area 24 is filled with blood samples by observing the blood samples entering the breathable area 25.

[0063] The present invention also provides a split test strip for photochemical analysis prepared by the above preparation method.

[0064] Taking the detection of hemoglobin as an example in this embodiment, the liquid reagent contains a hemolytic agent. When the split test strip of the present invention is used, the liquid absorption port 21 is aligned with the liquid absorption position. Under the action of the siphon effect and the first hydrophilic cover plate 14, the blood sample flows into the reagent storage area 22 from the liquid absorption port 21 and dissolves the freeze-dried powder reagent in the reagent storage area 22. The red blood cells in the blood sample are destroyed to release hemoglobin; then, under the action of the siphon effect and the second hydrophilic cover plate 34, the blood sample reaches the breathable area 25 along the test flow channel 23 and the test area 24, and the gas is discharged to the outside through the air hole 35; then the split test strip is placed into an optical detection instrument to irradiate and detect the blood sample in the test area 24.

[0065] The above is only a preferred embodiment of the present invention. Therefore, any equivalent changes or modifications made according to the structure, characteristics and principles described in the scope of the present invention's patent application are included in the scope of the present invention's patent application.

Claims

1. A preparation method of a split test strip for photochemical analysis, characterized in that, It includes the following steps: S1. Fabricate a liquid absorption groove (11) and a reagent storage groove (12) that are sequentially connected on the bottom surface of the reagent flow channel module (1); S2. Place the reagent flow channel module (1) of step S1 on the bottom plate (2) such that the liquid absorption groove (11) and the bottom plate (2) enclose a liquid absorption port (21), and the reagent storage groove (12) and the bottom plate (2) enclose a reagent storage area (22); S3. Inhale a liquid reagent through the liquid absorption port (21). After the liquid reagent fills the reagent storage area (22), start the freeze-drying process to form a freeze-dried powder reagent in the reagent storage area (22); S4. Fabricate a communication groove (31) and a test groove (32) that are sequentially connected on the bottom surface of the test flow channel module (3); S5. Place the test flow channel module (3) of step S4 on the bottom plate (2) such that the communication groove (31) and the bottom plate (2) enclose a test flow channel (23), and the test groove (32) and the bottom plate (2) enclose a test area (24); Align and connect the test flow channel module (3) with the reagent flow channel module (1) to connect the test flow channel (23) with the reagent storage area (22).

2. The preparation method of the split test strip for photochemical analysis according to claim 1, characterized in that The specific steps of step S1 include the following steps: S11. Cut first openings (131) corresponding to the liquid absorption groove (11) and the reagent storage groove (12) on the first double-sided adhesive layer (13); S12. Paste the first double-sided adhesive layer (13) cut in step S11 on the bottom surface of the first hydrophilic cover plate (14).

3. The preparation method of the split test strip for photochemical analysis according to claim 2, wherein The first hydrophilic cover plate (14) is set as a first hydrophilic transparent plate; the first double-sided adhesive layer (13) is set as a first opaque layer.

4. The preparation method of the split test strip for photochemical analysis according to claim 1, characterized in that, The bottom plate (2) in step S2 is set as a hydrophobic plate.

5. The preparation method of the split test strip for photochemical analysis according to claim 1, characterized in that, The specific steps of step S4 include the following steps: S41. Cut second openings (331) corresponding to the communication groove (31) and the test groove (32) on the second double-sided adhesive layer (33); S42. Paste the second double-sided adhesive layer (33) cut in step S41 on the bottom surface of the second hydrophilic cover plate (34).

6. The preparation method of the split test strip for photochemical analysis according to claim 5, characterized in that, The second hydrophilic cover plate (34) is set as a second hydrophilic transparent plate; the second double-sided adhesive layer (33) is set as a second opaque layer.

7. The preparation method of the split test strip for photochemical analysis according to claim 1, characterized in that, Step S4 further includes opening an air hole (35) on the test flow channel module (3) that communicates with the test groove (32).

8. The preparation method of the split test strip for photochemical analysis according to claim 7, characterized in that, Step S4 further includes fabricating a breathable groove (36) on the bottom surface of the test flow channel module (3) that communicates with the test groove (32); the breathable groove (36) is used to enclose a breathable area (25) with the bottom plate (2).

9. The preparation method of the split test strip for photochemical analysis according to claim 8, characterized in that, The area of the breathable area (25) is smaller than the area of the test area (24).

10. A split test strip prepared by the method for preparing a split test strip for photochemical analysis according to any one of claims 1-9.

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