Liquid chromatograph, component separation method, storage medium and product

Abstract

The invention discloses a liquid chromatograph, a component separation method, a storage medium and a product, and relates to the technical field of mixture separation, the provided liquid chromatograph comprises a solvent and flow path module, the solvent and flow path module comprises a metering pump and at least one mobile phase liquid storage bottle; the sample separation module comprises a sample injector and a chromatographic column; the component detection module comprises a detector; the component collecting module comprises a plurality of middle collecting bottles and at least one target collecting bottle; the main control module at least comprises a parameter prediction model, and the parameter prediction model is used for outputting separation parameters based on the to-be-separated sample and controlling the solvent and flow path module, the sample separation module, the component detection module and the component collection module. The invention aims to solve the technical problem of how to improve the working efficiency of the liquid chromatograph.

Description

Technical Field

[0001] This application relates to the technical field of mixture separation, and particularly to a liquid chromatograph, a component separation method, a storage medium, and a product. Background Art

[0002] A liquid chromatograph is a preparation instrument used for separating, analyzing, and purifying each component in a mixture. It enables the separation of each component by allowing the mixture to flow through a chromatographic column filled with a stationary phase and utilizing the distribution differences of different components between the stationary phase and the mobile phase.

[0003] It can be understood that during the process of separating components by a liquid chromatograph, the separation of components is usually automatically achieved through the cooperation of the mobile phase and the chromatographic column. However, before separating the components, it is necessary to preset separation parameters such as the type of stationary phase and the proportion of the mobile phase solvent in advance. After separation, it is also necessary to manually analyze the products in each collection bottle and manually combine multiple fractions containing the target compound. Therefore, traditional liquid chromatographs still cannot avoid the participation of humans, resulting in relatively low working efficiency of the liquid chromatograph.

[0004] Therefore, how to improve the working efficiency of a liquid chromatograph is a technical problem that needs to be urgently solved by those skilled in the art. Summary of the Invention

[0005] The main objective of this application is to provide a liquid chromatograph, a component separation method, a storage medium, and a product, aiming to solve the technical problem of how to improve the working efficiency of a liquid chromatograph.

[0006] To achieve the above objective, this application proposes a liquid chromatograph, which includes:

[0007] A solvent and flow path module, which includes a metering pump and at least one mobile phase storage bottle, and the metering pump is connected to the output port of the mobile phase storage bottle;

[0008] A sample separation module, which includes an injector and a chromatographic column, one end of the injector is connected to the output port of the metering pump, and the other end of the injector is connected to the input port of the chromatographic column;

[0009] A component detection module, which includes a detector, and the input port of the detector is connected to the output port of the chromatographic column;

[0010] A component collection module, which includes a plurality of intermediate collection bottles and at least one target collection bottle, the intermediate collection bottles are connected to the output port of the detector and the target collection bottle, the intermediate collection bottles are used for sub-packaging the components output from the output port of the detector, and the target collection bottle is used for combining the components collected by the intermediate collection bottles;

[0011] A main control module, which is communicatively connected to the solvent and flow path module, the sample separation module, the component detection module, and the component collection module; the main control module at least includes a parameter prediction model for outputting separation parameters based on the molecular structure of the sample to be separated; the main control module is further configured to control the solvent and flow path module to output a mobile phase according to the separation parameters, and control the sample separation module, the component detection module, and the component collection module to achieve component separation.

[0012] In one embodiment, the liquid chromatograph further includes:

[0013] A mobile phase detection sensor, an input port of the mobile phase detection sensor is connected to an output port of the mobile phase storage bottle, and an output port of the mobile phase detection sensor is connected to an input end of the metering pump;

[0014] The main control module is also communicatively connected to the mobile phase detection sensor, and the main control module is further configured to output a stop separation instruction to the metering pump when it detects that the data collected by the mobile phase detection sensor indicates that the mobile phase storage bottle is in an empty bottle state.

[0015] In one embodiment, the liquid chromatograph further includes:

[0016] A first air pump, the first air pump is communicatively connected to the main control module;

[0017] A first one-way valve, an input port of the first one-way valve is connected to an output port of the first air pump, and an output port of the first one-way valve is connected to a pipeline between the detector and the chromatographic column;

[0018] The main control module is further configured to control the first air pump to output air to avoid residual liquid in the pipeline between the detector and the chromatographic column.

[0019] In one embodiment, the liquid chromatograph further includes:

[0020] A second one-way valve, an input port of the second one-way valve is connected to an output port of the chromatographic column;

[0021] A pressure sensor, one end of the pressure sensor is connected to an output port of the second one-way valve, and the other end of the pressure sensor is connected to an input port of the detector;

[0022] The main control module is also communicatively connected to the pressure sensor, and the main control module is further configured to detect the pipeline blockage state according to the pressure collected by the pressure sensor.

[0023] In one embodiment, the liquid chromatograph further includes:

[0024] An automatic cleaning module, the automatic cleaning module includes a diaphragm pump, a switching valve, a spray liquid storage bottle and a waste liquid storage bottle. One end of the diaphragm pump is connected to the output port of the spray liquid storage bottle, the other end of the diaphragm pump is connected to one end of the switching valve, the other end of the switching valve is connected to one end of the intermediate collection bottle, and the other end of the intermediate collection bottle is connected to the waste liquid storage bottle;

[0025] The main control module is also communicatively connected to the switching valve, and the main control module is also used to transmit an opening instruction or a closing instruction to the switching valve, so as to clean the intermediate collection bottle with the spray liquid in the spray liquid storage bottle, and collect the spray liquid after cleaning the collection bottle through the waste liquid storage bottle.

[0026] In one embodiment, the liquid chromatograph further includes:

[0027] A second air pump, the second air pump is communicatively connected to the main control module;

[0028] A third one-way valve, the input port of the third one-way valve is connected to the output port of the second air pump;

[0029] A first solenoid valve, the input end of the first solenoid valve is connected to the output port of the third one-way valve and the output port of the metering pump, the first output port of the first solenoid valve is connected to the input port of the injector, and the second output port of the first solenoid valve is connected to the output port of the chromatographic column;

[0030] The main control module is also used to control the second air pump to output air to clean the chromatographic column and discharge the residual liquid.

[0031] In addition, to solve the above problems, the present application also proposes a component separation method, and the component separation method is applied to the main control module of the liquid chromatograph as described above;

[0032] The component separation method includes:

[0033] Obtaining separation parameters of a sample to be separated through a parameter prediction model in the main control module, and controlling the solvent and flow path module to output a mobile phase through the separation parameters;

[0034] Controlling the sample separation module to separate the sample to be separated containing the mobile phase, and controlling the component detection module to detect the liquid separated by the sample separation module to obtain detection data;

[0035] Controlling the intermediate collection bottle in the component collection module to collect the liquid, and determining the serial number of the intermediate collection bottle corresponding to each component based on the detection data;

[0036] For each component, use the intermediate collection bottle corresponding to the intermediate collection bottle number of the component as the collection bottle to be emptied, and transfer the liquid in the collection bottle to be emptied to the target collection bottle preset for the component, so as to obtain each separated component.

[0037] In one embodiment, the detection data includes an elution curve;

[0038] The step of determining the intermediate collection bottle number corresponding to each component based on the detection data includes:

[0039] Based on the elution curve, determine the peak elution time of each component in the sample to be separated;

[0040] For each component, according to the peak elution time corresponding to the component and the elution curve, determine the start collection time and the end collection time corresponding to the component;

[0041] For each component, according to the start collection time and the end collection time corresponding to the component, determine the intermediate collection bottle number corresponding to the component, so as to obtain the intermediate collection bottle number corresponding to each component.

[0042] In addition, to achieve the above object, the present application also proposes a storage medium, the storage medium is a computer-readable storage medium, and a computer program is stored on the storage medium. When the computer program is executed by a processor, the steps of the component separation method described above are implemented.

[0043] In addition, to achieve the above object, the present application also provides a computer program product, the computer program product includes a computer program, and when the computer program is executed by a processor, the steps of the component separation method described above are implemented.

[0044] One or more technical solutions proposed by the present application have at least the following technical effects:

[0045] The present application can automatically predict the separation parameters required for the sample to be separated through the parameter prediction model in the main control module, and then can control the solvent and flow path module to output the mobile phase according to the separation parameters through the communication connection between the main control module and the solvent and flow path module. During the separation process, the main control module can also control the solvent and flow path module, the sample separation module, the component detection module, and the component collection module through the communication connection between the modules. Thus, the present application can control the solvent and flow path module, the sample separation module, the component detection module, and the component collection module through the main control module, so that the component separation process is controlled by the main control module itself, realizing the full automation of the separation process. Compared with the traditional technology, since the present application does not require manual participation, the working efficiency of the liquid chromatograph is improved. Description of the Drawings

[0046] The accompanying drawings here are incorporated into the specification and form a part of this specification, showing embodiments consistent with this application, and are used together with the specification to explain the principles of this application.

[0047] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings required for use in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those of ordinary skill in the art, without creative efforts, other accompanying drawings can also be obtained based on these drawings.

[0048] Figure 1 It is a schematic diagram of the framework of the first embodiment of the liquid chromatograph of this application;

[0049] Figure 2 It is a schematic diagram of the framework of the second embodiment of the liquid chromatograph of this application;

[0050] Figure 3 It is a schematic diagram of the architecture of a specific embodiment of the liquid chromatograph of this application;

[0051] Figure 4 It is a schematic diagram of the architecture of the third embodiment of the liquid chromatograph of this application;

[0052] Figure 5 It is a schematic diagram of the process of the first embodiment of the component separation method of this application;

[0053] Figure 6 It is a schematic diagram of the process of applying the parameter prediction model in an embodiment of the component separation method of this application;

[0054] Figure 7 It is a schematic diagram of the scenario of an embodiment of the component separation method of this application;

[0055] Figure 8 It is a schematic diagram of the process of a specific embodiment of the component separation method of this application;

[0056] The realization of the purpose, functional features, and advantages of this application will be further described with reference to the embodiments and the accompanying drawings.

[0057] Explanation of the reference numerals in the accompanying drawings:

[0058] 10. Solvent and Flow Path Module; 101. Metering Pump; 102. Mobile Phase Reservoir; 20. Sample Separation Module; 201. Injector; 202. Chromatographic Column; 30. Component Detection Module; 301. Detector; 40. Component Collection Module; 401. Intermediate Collection Bottle; 402. Target Collection Bottle; 50. Main Control Module; 60. Mobile Phase Detection Sensor; 70. First Air Pump; 80. First Check Valve; 90. Second Check Valve; 100. Pressure Sensor; 110. Diaphragm Pump; 1101. Switch Valve; 1102. Spraying Liquid Reservoir; 1103. Waste Liquid Reservoir. Detailed Embodiment

[0059] It should be understood that the specific embodiments described herein are only used to explain the technical solutions of the present application and are not used to limit the present application.

[0060] In order to better understand the technical solutions of the present application, the following will be described in detail in conjunction with the accompanying drawings of the specification and specific embodiments.

[0061] A liquid chromatograph is a preparative instrument used to separate, analyze, and purify each component in a mixture. It separates each component by allowing the mixture to flow through a chromatographic column filled with a stationary phase and utilizing the distribution differences of different components between the stationary phase and the mobile phase.

[0062] It can be understood that during the process of separating components by a liquid chromatograph, the separation of components is usually automatically achieved through the cooperation of the mobile phase and the chromatographic column. However, before separating the components, it is necessary to preset separation parameters such as the type of stationary phase and the proportion of the mobile phase solvent in advance. After separation, it is also necessary to manually analyze the products in each collection bottle and manually combine multiple fractions containing the target compound. Therefore, traditional liquid chromatographs still cannot avoid manual participation, resulting in low working efficiency of the liquid chromatograph.

[0063] Therefore, how to improve the working efficiency of a liquid chromatograph is a technical problem that those skilled in the art urgently need to solve.

[0064] To solve the above problems, the present application proposes a liquid chromatography instrument, which includes: a solvent and flow path module, the solvent and flow path module includes a metering pump and at least one mobile phase reservoir, and the metering pump is connected to the output port of the mobile phase reservoir; a sample separation module, the sample separation module includes an injector and a chromatographic column, one end of the injector is connected to the output port of the metering pump, and the other end of the injector is connected to the input port of the chromatographic column; a component detection module, the component detection module includes a detector, and the input port of the detector is connected to the output port of the chromatographic column; a component collection module, the component collection module includes a plurality of intermediate collection bottles and at least one target collection bottle, the intermediate collection bottles are connected to the output port of the detector and the target collection bottle, the intermediate collection bottles are used for dispensing the components output from the output port of the detector, and the target collection bottle is used for combining the components collected by the intermediate collection bottles; a main control module, the main control module is communicatively connected to the solvent and flow path module, the sample separation module, the component detection module and the component collection module; the main control module at least includes a parameter prediction model for outputting separation parameters based on the molecular structure of the sample to be separated; the main control module is further configured to control the solvent and flow path module to output the mobile phase according to the separation parameters, and control the sample separation module, the component detection module and the component collection module to achieve component separation.

[0065] The present application can automatically predict the separation parameters required for the sample to be separated through the parameter prediction model in the main control module, and then can control the solvent and flow path module to output the mobile phase according to the separation parameters through the communication connection between the main control module and the solvent and flow path module. During the separation process, the main control module can also control the solvent and flow path module, the sample separation module, the component detection module and the component collection module through the communication connection between the modules. Thus, the present application can control the solvent and flow path module, the sample separation module, the component detection module and the component collection module through the main control module, so that the component separation process is self-controlled by the main control module, realizing the full automation of the separation process. Compared with the traditional technology, since the present application does not require manual participation, the working efficiency of the liquid chromatography instrument is improved.

[0066] Please refer to Figure 1 , Figure 1 which is a schematic framework diagram of the first embodiment of the liquid chromatography instrument of the present application.

[0067] In Figure 1 , the liquid chromatography instrument includes:

[0068] A solvent and flow path module 10, the solvent and flow path module 10 includes a metering pump 101 and at least one mobile phase reservoir 102, and the metering pump 101 is connected to the output port of the mobile phase reservoir 102;

[0069] Sample separation module 20, the sample separation module 20 includes a sampler 201 and a chromatographic column 202. One end of the sampler 201 is connected to the output port of the metering pump 101, and the other end of the sampler 201 is connected to the input port of the chromatographic column 202;

[0070] Component detection module 30, the component detection module 30 includes a detector 301, and the input port of the detector 301 is connected to the output port of the chromatographic column 202;

[0071] Component collection module 40, the component collection module 40 includes a plurality of intermediate collection bottles 401 and at least one target collection bottle 402. The intermediate collection bottles 401 are connected to the output port of the detector 301 and the target collection bottle 402. The intermediate collection bottles 401 are used for sub-packaging the components output from the output port of the detector 301, and the target collection bottle 402 is used for combining the components collected by the intermediate collection bottles 401;

[0072] Main control module 50, the main control module 50 is communicatively connected to the solvent and flow path module 10, the sample separation module 20, the component detection module 30, and the component collection module 40; the main control module 50 at least includes a parameter prediction model for outputting separation parameters based on the molecular structure of the sample to be separated; the main control module 50 is also used to control the solvent and flow path module 10 to output the mobile phase according to the separation parameters, and control the sample separation module 20, the component detection module 30, and the component collection module 40 to achieve component separation.

[0073] It can be understood that the solvent and flow path module 10 is mainly used to control the output of the mobile phase. Among them, there is at least one type of mobile phase, so there is at least one mobile phase storage bottle 102 in the solvent and flow path module 10. The metering pump 101 is arranged at the outlet of the mobile phase storage bottle 102, and is specifically used to control the flow rate of the mobile phase and the ratio between multiple mobile phases. Preferably, the metering pump 101 can be a four-in-one high-pressure constant flow pump. When using the gradient elution method, a quaternary gradient valve pump can be preferably used.

[0074] The sample separation module 20 is mainly used to separate each component. Specifically, the injector 201 is used to inject the sample to be separated, and the chromatographic column 202 is used to separate the components of the sample to be separated according to the stationary phase built therein and the flowing mobile phase. Since the chromatographic column 202 usually separates components by flow rate and adsorption force, the separated components will flow out of the chromatographic column 202 at different times. In a feasible implementation manner, the injector 201 can be an automatic injector, that is, the injector 201 can be an automatic injector 201. Specifically, the automatic injector 201 includes a sample loop and a six-way valve structure, which are used to accurately quantify the sample liquid and then switch it into the flow path. Compared with the traditional manual injection method, the automatic injector 201 can ensure the consistency of the sample loading amount and accurate position each time, avoiding the errors and sample exposure problems of manual injection. In addition, the chromatographic column 202 proposed in this application can be silica gel columns, ion exchange columns, etc. of different sizes, and this application does not limit this.

[0075] The component detection module 30 mainly includes a detector 301. The detector 301 is used to detect the components in the liquid to facilitate the measurement of the components. It can be understood that since different components are separated at different times, in order to determine the specific type of the component flowing out at the current moment, it is also necessary to detect the liquid flowing out at the current moment through the detector 301. Specifically, the detector 301 can be a UV-Vis ultraviolet detector 301, so that the ultraviolet light absorption of the liquid flowing out currently can be detected through the UV-Vis ultraviolet detector 301, and then the data characterizing the ultraviolet light absorption is transmitted to the main control module 50, so that the main control module 50 can identify the component at the current moment.

[0076] The component collection module 40 includes two types of collection bottles. Among them, the intermediate collection bottle 401 is used to store the liquid flowing out in each time period, and the target collection bottle 402 is mainly used to collect the liquid containing specific components. It can be understood that for a certain component, its separation process is continuous, and for a certain moment, it is not known whether the component has been separated. Therefore, it is necessary to temporarily store the separated liquid through the intermediate collection bottle 401, and then determine the start collection time and end collection time of the component through the data detected by the detector 301, and then collect the liquid in the intermediate collection bottle 401 corresponding to the time period between the start collection time and the end collection time into the target collection bottle 402, so as to achieve the purpose of separating the component.

[0077] In addition, it can be understood that the main control module 50 of the present application can be a computer, a server, or other electronic devices with external control functions. The present application does not limit this. In this embodiment, the main control module 50 can be connected to each controllable hardware in the liquid chromatograph through technologies such as CAN bus, serial port, and Ethernet, so as to independently control each hardware to achieve the purpose of automatic separation. Specifically, a parameter prediction model for predicting separation parameters is preset in the main control module 50. Thus, before separating a certain sample to be separated, the molecular structure of the sample to be separated can be processed through the parameter prediction model, so as to predict the separation parameters, and then output the separation parameters with the highest separation probability. Then, the separation parameters can be transmitted to the solvent and flow path module 10 that controls the separation conditions through technologies such as serial port and CAN bus, so that the solvent and flow path module 10 can automatically output the mobile phase according to the separation parameters.

[0078] In addition, it should also be noted that the main control module 50 in the liquid chromatograph can also obtain the working parameters of each hardware during the separation process (such as the ultraviolet absorption of the UV-Vis ultraviolet detector 301, the flow rate set by the metering pump 101, etc.) through its connection with each hardware, and then flexibly adjust the separation strategy according to the working parameters, and then control the sample separation module 20, the component detection module 30, and the component collection module 40 to improve the separation efficiency.

[0079] In this embodiment, the present application can automatically predict the separation parameters required for the sample to be separated through the parameter prediction model in the main control module 50, and then can control the solvent and flow path module 10 to output the mobile phase according to the separation parameters through the communication connection between the main control module 50 and the solvent and flow path module 10 to separate the components. In addition, during the separation process, the main control module 50 can also obtain the working parameters collected by the solvent and flow path module 10, the sample separation module 20, the component detection module 30, and the component collection module 40 through the communication connection between the modules, and then adjust the separation strategy during the separation process in real time according to the collected working parameters. Thus, the present application can control the solvent and flow path module 10, the sample separation module 20, the component detection module 30, and the component collection module 40 through the software layer included in the main control module 50, so as to realize the full automation of the separation process. Compared with the traditional technology, since the present application does not require manual participation, the working efficiency of the liquid chromatograph is improved.

[0080] Further, based on the first embodiment of the liquid chromatograph of the present application, a second embodiment of the liquid chromatograph of the present application is proposed.

[0081] Please refer to Figure 2 , Figure 2Schematic diagram of the framework of the second embodiment of the liquid chromatograph of the present application. In this embodiment, the liquid chromatograph further includes:

[0082] A mobile phase detection sensor 60, the input port of the mobile phase detection sensor 60 is connected to the output port of the mobile phase storage bottle 102, and the output port of the mobile phase detection sensor 60 is connected to the input end of the metering pump 101;

[0083] The main control module 50 is also communicatively connected to the mobile phase detection sensor 60, and the main control module 50 is further configured to output a stop separation instruction to the metering pump 101 when detecting that the data collected by the mobile phase detection sensor 60 indicates that the mobile phase storage bottle 102 is in an empty bottle state.

[0084] It can be understood that the mobile phase detection sensor 60 provided between the metering pump 101 and the mobile phase storage bottle 102 in this embodiment is mainly used to determine whether there is still mobile phase in the mobile phase storage bottle 102, and then a stop separation instruction can be output to the metering pump 101 when the mobile phase storage bottle 102 is empty, so as to avoid the problem of separation failure caused by insufficient mobile phase. In one embodiment, the mobile phase detection sensor 60 can be a bubble sensor or other sensors, and the present application does not limit this.

[0085] In one embodiment, the liquid chromatograph further includes:

[0086] A first air pump 70, the first air pump 70 is communicatively connected to the main control module 50;

[0087] A first one-way valve 80, the input port of the first one-way valve 80 is connected to the output port of the first air pump 70, and the output port of the first one-way valve 80 is connected to the pipeline between the detector 301 and the chromatographic column 202;

[0088] The main control module 50 is further configured to control the first air pump 70 to output air to avoid residual liquid in the pipeline between the detector 301 and the chromatographic column 202.

[0089] It can be understood that after detecting the end of separation, the main control module 50 can also control the first air pump 70 to output air. Then, the first air pump 70 will input air into the pipeline between the detector 301 and the chromatographic column 202 through the first one-way valve 80, increasing the pressure inside the pipeline, so as to output the residual liquid inside the pipeline and avoid the problem of pipeline contamination caused by liquid residue.

[0090] In addition, in this embodiment, the above-mentioned first one-way valve 80 is used to prevent liquid from flowing back into the output port of the first air pump 70.

[0091] In one embodiment, the liquid chromatograph further includes:

[0092] A second one-way valve 90, the input port of the second one-way valve 90 is connected to the output port of the chromatographic column 202;

[0093] A pressure sensor 100, one end of the pressure sensor 100 is connected to the output port of the second one-way valve 90, and the other end of the pressure sensor 100 is connected to the input port of the detector 301;

[0094] The main control module 50 is also communicatively connected to the pressure sensor 100, and the main control module 50 is further configured to detect the blockage state of the pipeline according to the pressure collected by the pressure sensor 100.

[0095] In this embodiment, the second one-way valve 90 is used to prevent the liquid output from the chromatographic column 202 from flowing back to the chromatographic column 202, and the pressure sensor 100 is used to detect the pressure between the detector 301 and the second one-way valve 90, so as to judge whether the pipeline is blocked based on the magnitude of the pressure. Furthermore, when it is detected that the pipeline is in a blocked state, the first air pump 70 can be controlled to output air.

[0096] In one embodiment, the liquid chromatograph further includes:

[0097] An automatic cleaning module, the automatic cleaning module includes a diaphragm pump 110, a switching valve 1101, a spray liquid storage bottle 1102 and a waste liquid storage bottle 1103. One end of the diaphragm pump 110 is connected to the output port of the spray liquid storage bottle 1102, and the other end of the diaphragm pump 110 is connected to one end of the switching valve 1101. The other end of the switching valve 1101 is connected to one end of the intermediate collection bottle 401, and the other end of the intermediate collection bottle 401 is connected to the waste liquid storage bottle 1103;

[0098] The main control module 50 is also communicatively connected to the switching valve 1101, and the main control module 50 is further configured to transmit an opening instruction or a closing instruction to the switching valve 1101, so as to clean the intermediate collection bottle 401 with the spray liquid in the spray liquid storage bottle 1102, and collect the spray liquid after cleaning the collection bottle through the waste liquid storage bottle 1103.

[0099] It can be understood that, in a feasible implementation manner, the switching valve 1101 can be a two-way valve. Since the target collection bottle 402 stores the separated components, the spray liquid is only used to clean the intermediate collection bottle 401.

[0100] In this embodiment, after the main control module 50 detects the end of separation, it can send an opening instruction to the switching valve 1101, so that the spraying liquid can start from the spraying liquid storage bottle 1102, flow through each intermediate collection bottle 401, and finally flow into the waste liquid storage bottle 1103, realizing the cleaning function of the intermediate collection bottle 401.

[0101] Please refer to Figure 3 , Figure 3 which is a schematic structural diagram of a specific embodiment of the liquid chromatograph of the present application. It should be noted that, for the convenience of viewing the connection relationships of each hardware, Figure 3 the main control module is not pointed out in Figure 3 . However, the following content is given on the premise that there is a communication connection between the main control module and the controllable hardware in

[0102] such as, for example, the four-in-one high-pressure constant flow pump, the pressure sensor, the detector and other hardware. Figure 3 In

[0103] , there are four mobile phases (mobile phases 1-4), and a mobile phase detection sensor is provided at the output port of each mobile phase storage bottle. The mobile phase for separating components will be input to the four-in-one high-pressure constant flow pump after passing through the mobile phase detection sensor. Then, the four-in-one high-pressure constant flow pump controls the flow rate and proportional relationship of the output mobile phase according to the separation parameters transmitted by the main control module, and outputs the mobile phase. The output mobile phase can be mixed with the sample to be separated input by the auto-sampler, and then enter the chromatographic column. The components separated in the chromatographic column will enter the detector through the one-way valve and the pressure sensor for detection, then flow out of the detector and pass through a mobile phase detection sensor, and then enter the intermediate collection bottle through the six-port valve and the solenoid valve. It should be noted that there are multiple intermediate collection bottles. When collecting liquid, the liquid can be injected into the intermediate collection bottles in sequence according to the arrangement order of the intermediate collection bottles, so that the liquids output at different time periods can be stored in different intermediate collection bottles.

[0104] It should be noted that, for the convenience of the liquid flowing into the target collection bottle, the air in the target collection bottle can also be extracted by the target diaphragm pump (the two-way valve between the target collection bottle and the target diaphragm pump is used to open or close the channel).

[0105] After the component separation, the residual liquid between the pressure sensor and the detector can be pressed into the waste liquid storage bottle by pressurization with the first air pump. The spraying liquid in the spraying liquid storage bottle can flow through the mobile phase detection sensor, the diaphragm pump, the two-way valve, and the six-way valve, flow through each intermediate collection bottle from top to bottom, and then flow through the solenoid valve and the six-way valve after flowing out of the intermediate collection bottle and enter the waste liquid storage bottle to achieve the purpose of cleaning.

[0106] Furthermore, based on the above-mentioned various embodiments of the liquid chromatograph of the present application, a third embodiment of the liquid chromatograph of the present application is proposed.

[0107] Please refer to Figure 4 , in this embodiment, the liquid chromatograph further includes:

[0108] A second air pump, which is communicatively connected to the main control module;

[0109] A third one-way valve, the input port of which is connected to the output port of the second air pump;

[0110] A first solenoid valve, the input end of which is connected to the output port of the third one-way valve and the output port of the metering pump, the first output port of the first solenoid valve is connected to the input port of the injector, and the second output port of the first solenoid valve is connected to the output port of the chromatographic column;

[0111] The main control module is further configured to control the second air pump to output air to clean the chromatographic column and discharge the residual liquid.

[0112] It can be understood that, compared with Figure 3 , the air pump (second air pump) in this embodiment is arranged upstream of the chromatographic column in the flowing direction of the mobile phase. Therefore, the chromatographic column and the pipeline after the chromatographic column can be cleaned by the air output by the second air pump, playing a cleaning role.

[0113] It should be noted that the above examples are only for understanding the present application and do not constitute a limitation to the liquid chromatograph of the present application. Based on this technical concept, more forms of simple transformation are within the protection scope of the present application.

[0114] Based on the above liquid chromatograph, an embodiment of the present application provides a component separation method, which is applied to the above main control module. Please refer to Figure 5 , Figure 5 is a schematic flowchart of the first embodiment of the component separation method of the present application.

[0115] In this embodiment, the component separation method includes steps S10 to S30:

[0116] Step S10: Obtain the separation parameters of the sample to be separated through the parameter prediction model in the main control module, and control the solvent and flow path module to output the mobile phase according to the separation parameters;

[0117] It should be noted that the parameter prediction model can be a neural network model or a machine learning model, and this application does not limit this. Specifically, the parameter prediction model can use artificial intelligence algorithms to learn a large amount of historical experimental data to form a model capable of predicting separation parameters, and provide decision-making suggestions during the experiment. For example, after the parameter prediction model gives the separation parameters, the main control module can generate a specific execution sequence according to the separation parameters: set the flow rate curves of different mobile phases to achieve the separation process of components with a specific gradient.

[0118] In a feasible implementation manner, the steps to construct the parameter prediction model can be: First, it is necessary to collect column chromatography experimental data through an automated high-throughput experimental platform. These data include information such as the molecular formula of the compound, experimental conditions (such as column specifications, sample loading mass, sample loading solvent and its mass, eluent system and ratio, flow rate, etc.), and separation time. Next, the collected data is cleaned and integrated to remove duplicates and outliers to obtain a complete and reliable column chromatography data set. Then, the data is preprocessed. The molecular formula of the compound is converted into a digital form, and molecular fingerprints and molecular descriptors are used to characterize its molecular structure and properties. At the same time, the eluent ratio vector is used to represent the eluent system and ratio, and finally an input information matrix for training the neural network model is generated. When constructing the basic prediction model, first construct an atom-bond graph (hereinafter referred to as graph G) and a bond length-bond angle graph (hereinafter referred to as graph H) for the compound molecule, and then construct a quantile geometric enhanced graph neural network (GeoGNN) based on these two graphs. Embed the experimental parameter features into the edge features of graph G and the molecular descriptors into the edge features of graph H, generate a specific graph representation through graph isomorphism convolution operations, and then train the basic prediction model based on a specific specification of the chromatographic column so that it can predict the column chromatography separation probability and separation time of the mixture under given experimental conditions. After that, in order to make the model applicable to chromatographic columns of multiple specifications, transfer learning is carried out in a parameter transfer manner. The network training parameters of the basic prediction model are transferred to the prediction model network of other specifications of chromatographic columns, and training is carried out using a lower learning rate to obtain a parameter prediction model that can adapt to different specifications of chromatographic columns.

[0119] Please refer to Figure 6 , Figure 6 which is a schematic diagram of the parameter prediction model application process for an embodiment of the component separation method of this application. In Figure 6Among them, technicians can confirm by themselves whether the chromatographic column needs to be replaced. On the premise that the chromatographic column needs to be replaced, the chromatographic column can be replaced, and then the molecular structure of the sample to be separated can be input into the parameter prediction model so that the model can predict the separation parameters. Of course, in a feasible implementation manner, the detection method for whether to replace the chromatographic column can also be that the main control module autonomously judges whether the solid phase of the chromatographic column is suitable for the sample to be separated, and then automatically replaces it. The present application does not limit the replacement method of the chromatographic column.

[0120] After receiving the separation parameters transmitted by the main control module, the solvent and flow path module can control the mobile phase ratio and flow rate according to the separation parameters and output the mobile phase.

[0121] Step S20: Control the sample separation module to separate the sample to be separated containing the mobile phase, and control the component detection module to detect the liquid separated by the sample separation module to obtain detection data.

[0122] It can be understood that the main control module can control the automatic sampler to inject samples automatically. Thus, the input sample to be separated and the mobile phase will be mixed together to obtain a mixture. Then, the chromatographic column in the sample separation module will separate the mixture, and the separated liquid will enter the detector in the component detection module through the pipeline. The detection data in the detector can be transmitted to the main control module through a communication connection.

[0123] Step S30: Control the intermediate collection bottle in the component collection module to collect the liquid, and based on the detection data, determine the serial number of the intermediate collection bottle corresponding to each component.

[0124] In this embodiment, multiple intermediate collection bottles in the component collection module can be stored in a multi-row test tube rack, and then the liquid flowing through the detector during the separation process can be collected in sequence according to the arrangement order of the test tube rack.

[0125] It can be understood that when different detectors detect the liquid, the output detection data is different. The detection data in the present application can be understood as the detection results obtained when the detector detects the liquid. The detection data can be transmitted to the main control module in a wired or wireless manner, so that the main control module can determine the serial number of the intermediate collection bottle corresponding to each component based on the detection data.

[0126] It should be noted that since the separation of components lasts for a period of time, generally, there are multiple serial numbers of the intermediate collection bottles corresponding to the components.

[0127] Step S40: For each component, use the intermediate collection bottle corresponding to the serial number of the intermediate collection bottle of the component as the collection bottle to be emptied, and transfer the liquid in the collection bottle to be emptied to the target collection bottle preset for the component to obtain each separated component.

[0128] It can be understood that after the main control module determines the serial numbers of the intermediate collection bottles corresponding to each component, the liquids collected by the intermediate collection bottles belonging to the same component can be combined into the target collection bottle corresponding to this component, so as to achieve the purpose of automatically separating the components.

[0129] In this embodiment, the cooperation between the main control module and other hardware modules in the liquid chromatograph makes the separation process of the liquid chromatograph fully automatic, thereby improving the working efficiency of the liquid chromatograph.

[0130] Furthermore, based on the first embodiment of the above component separation method, a second embodiment of the component separation method of the present application is proposed.

[0131] In this embodiment, the detection data includes an elution curve; the above step S30 includes:

[0132] Step S301, based on the elution curve, determine the peak times of the respective components in the sample to be separated;

[0133] It should be noted that the elution curve is a curve with time as the abscissa and the detector response signal as the ordinate, which reflects the signal change when each component in the sample to be separated flows out of the detector in sequence after being separated by the chromatographic column. The peak time refers to the acquisition time corresponding to the peak signal (peak value) in the elution curve. In this embodiment, the peak signals of each component can be detected through a model or an image processing algorithm, and then the peak times corresponding to each component can be determined.

[0134] Step S302, for each component, based on the peak time corresponding to this component and the elution curve, determine the start collection time and the end collection time corresponding to this component;

[0135] It can be understood that the number of peak shapes in the elution curve is the same as the number of component types, that is, one component corresponds to one waveform in the elution curve. On the basis of obtaining the peak times corresponding to each component, when determining the start collection time and the end collection time of a certain component, the detector signal parameters corresponding to the peak time of this component in the elution curve can be obtained first, and then the signal parameters corresponding to the peak time are used as the target parameter values, and the signal parameter values corresponding to the times before and after the peak time are respectively subtracted from the target parameter values, and the collection time of the signal parameter value corresponding to the maximum difference before the peak time is used as the start collection time, and the collection time of the signal parameter value corresponding to the maximum difference after the peak time is used as the end collection time.

[0136] Step S303: For each component, determine the serial number of the intermediate collection bottle corresponding to the component according to the start collection time and end collection time corresponding to the component, so as to obtain the serial number of the intermediate collection bottle corresponding to each component.

[0137] It can be understood that each intermediate collection bottle containing liquid has its own start collection time and end collection time (hereinafter referred to as "sub-start collection time" and "sub-end collection time" for the convenience of distinguishing from the start collection time and end collection time of the component). Therefore, when determining the serial number of the intermediate collection bottle corresponding to a certain component, the serial number of the intermediate collection bottle whose [sub-start collection time, sub-end collection time] is within the interval of [start collection time, end collection time] can be used as the serial number of the intermediate collection bottle corresponding to the component.

[0138] In this embodiment, the present application can autonomously achieve the sub-packaging of components through the main control module, thereby avoiding the problem of low work efficiency caused by manual sub-packaging.

[0139] In a feasible implementation manner, when the parameter prediction module gives the separation parameters, it will simultaneously give the prediction of the separation process of the component. If an abnormal situation inconsistent with the prediction of the parameter prediction model is detected in the experiment, the main control module also has the ability of immediate decision-making, such as measures to extend the elution time, increase the gradient steepness or repeat the injection and separation (these rules can be set in advance by expert experience). Thus, through real-time data closed-loop control, the present application can not only execute the program step by step, but also make a certain degree of autonomous response to the changing situation, improving the success rate and safety of component separation.

[0140] In addition, in a feasible implementation manner, if the prediction uncertainty of the parameter prediction model for a certain sample to be separated is relatively high, a small-scale column test run experiment (such as using a 4g test column) can be carried out first to obtain the measured retention data, and then the parameter prediction model can be adjusted for large-scale column purification. Compared with the above-mentioned embodiments, this is equivalent to replacing a large-scale separation with a two-step process of "test + formal", so the success rate and reliability can also be ensured.

[0141] In addition, the main control module can also provide an intuitive GUI interface for users. Specifically, users can input experiment requests on the interface (such as selecting samples and setting special requirements), view the solutions recommended by the AI model and confirm them. After the experiment starts, the interface can display key status in real time: for example, the time axis of chromatographic operation, the current gradient ratio, the dynamic update of the detector chromatogram, and which components have been collected, etc. During the separation process, users do not need to intervene, but can understand the progress through the interface at any time. Moreover, users can also send control instructions (such as emergency stop) through the interface. In addition, the main control module also supports remote monitoring and cloud data synchronization. Users can view the running status on an office computer or even a mobile device, or the cloud server can use the new data for model retraining. In addition, the interface also provides an experimental report export function. The conditions and results of each run will automatically generate a log, which is convenient for traceability and optimization adjustment.

[0142] In a feasible implementation, the main control module can also be responsible for the safety monitoring of each module. For example, the main control module can automatically stop the pump and release pressure when the pressure is too high, etc., to ensure the reliable and stable operation of the system.

[0143] Please refer to Figure 7 , Figure 7 which is a schematic diagram of the scenario of an embodiment of the component separation method of this application. In Figure 4 , the parameter prediction model integrated in the main control module can provide prediction services when users use the instrument. At the same time, during the interaction process between the main control module and each hardware in the liquid chromatograph, the functions of the main control module and the prediction function of the parameter prediction model can also be updated and iterated through the generated data to improve the prediction accuracy of the model. Therefore, in a feasible implementation, the main control module can also undertake the data management function, store the raw data and results generated by each experiment in the local database or upload them to the cloud, and fuse with the data for constructing the parameter prediction model. When a certain amount of new data accumulates, the model update program can be triggered during idle time to perform incremental training or transfer learning on the AI prediction model (especially after replacing a new column or separating new types of compounds, the model can be appropriately adjusted to adapt). The model update of this application can be selected by users to be automatic or manual, and this application does not limit this. In this embodiment, through the integrated management of data-model-experiment, the main control module can continuously improve itself, so as to achieve the effect of becoming more efficient and intelligent with use.

[0144] This application abstracts the complex hardware operation process into an automated process directed by software. It can shield details from users and provide a good experience for users above, and can accurately control the instrument to achieve intelligent operation below. This design ensures that the wisdom of the AI model and the execution power of the mechanical device can be fully combined, providing guarantee for realizing the target function of this application.

[0145] Please refer to Figure 8 , Figure 8 which is a schematic flow diagram of a specific embodiment of the component separation method of the present application.

[0146] In this embodiment, after the main control module predicts the separation parameters based on the molecular structure of the sample to be separated input by the user, it can send a start separation instruction to each hardware. After the separation is completed, it can determine the peak emergence time of each component through the detection data of the detector, and then merge the liquids in multiple intermediate collection bottles according to the peak emergence time, so as to achieve the purpose of automatic collection. After the components are collected, the remaining liquid in the tube can be discharged into the waste liquid storage bottle by the first air pump, and the intermediate collection bottle can be cleaned with the spraying liquid and reset after cleaning. Furthermore, the experiment can be ended after the waste liquid is cleared.

[0147] It should be noted that the above examples are only for understanding the present application and do not constitute a limitation to the component separation method of the present application. Based on this technical concept, more forms of simple transformations are within the protection scope of the present application.

[0148] The present application provides a computer-readable storage medium having computer-readable program instructions (i.e., computer programs) stored thereon, and the computer-readable program instructions are used to execute the component separation method in the above embodiment.

[0149] The computer-readable storage medium provided by the present application may be, for example, a USB flash drive, but is not limited to electrical, magnetic, optical, electromagnetic, infrared, or semiconductor systems or devices, or any combination of the above. More specific examples of the computer-readable storage medium may include, but are not limited to: an electrical connection having one or more wires, a portable computer disk, a hard disk, a random access memory (RAM), a read-only memory (ROM), an erasable programmable read-only memory (EPROM) or a flash memory, an optical fiber, a portable compact disk read-only memory (CD-ROM), an optical storage device, a magnetic storage device, or any suitable combination of the above. In this embodiment, the computer-readable storage medium may be any tangible medium that contains or stores a program, and the program can be used by or in combination with an instruction execution system or device. The program code contained on the computer-readable storage medium can be transmitted by any appropriate medium, including but not limited to: wires, optical cables, RF (Radio Frequency), etc., or any suitable combination of the above.

[0150] The above computer-readable storage medium may be included in the main control module; or it may exist independently and not be assembled into the main control module.

[0151] The above computer-readable storage medium carries one or more programs. When the one or more programs are executed by the main control module, the main control module is caused to: obtain separation parameters of a sample to be separated through a parameter prediction model in the main control module, and control the solvent and flow path module to output a mobile phase through the separation parameters; control the sample separation module to separate the sample to be separated containing the mobile phase, and control the component detection module to detect the liquid separated by the sample separation module to obtain detection data; control an intermediate collection bottle in the component collection module to collect the liquid, and determine the serial number of the intermediate collection bottle corresponding to each component based on the detection data; for each component, use the intermediate collection bottle corresponding to the serial number of the intermediate collection bottle of the component as a collection bottle to be emptied, and transfer the liquid in the collection bottle to be emptied to the target collection bottle preset for the component, so as to obtain each separated component.

[0152] Computer program code for performing the operations of the present application may be written in one or more programming languages or combinations thereof. The above programming languages include object-oriented programming languages such as Java, Smalltalk, C++, and also include conventional procedural programming languages such as the "C" language or similar programming languages. The program code may be executed entirely on the user's computer, partially on the user's computer, executed as a stand-alone software package, partially on the user's computer and partially on a remote computer, or entirely on a remote computer or server. In the case of a remote computer, the remote computer may be connected to the user's computer through any type of network, including a local area network (LAN) or a wide area network (WAN), or may be connected to an external computer (for example, by using an Internet service provider to connect through the Internet).

[0153] The flowcharts and block diagrams in the accompanying drawings illustrate the possible architectures, functions, and operations of systems, methods, and computer program products according to various embodiments of the present application. In this regard, each block in the flowchart or block diagram may represent a module, a segment of a program, or a part of code that contains one or more executable instructions for implementing the specified logical function. It should also be noted that in some alternative implementations, the functions marked in the blocks may occur in a different order than that marked in the accompanying drawings. For example, two consecutive blocks shown may actually be executed substantially in parallel, and they may sometimes be executed in the reverse order, depending on the functions involved. It should also be noted that each block in the block diagram and / or flowchart, as well as combinations of blocks in the block diagram and / or flowchart, can be implemented by a dedicated hardware-based system that performs the specified functions or operations, or can be implemented by a combination of dedicated hardware and computer instructions.

[0154] The modules described in the embodiments of the present application can be implemented in software or in hardware. In some cases, the name of the module does not constitute a limitation on the unit itself.

[0155] The readable storage medium provided by the present application is a computer-readable storage medium, and the computer-readable storage medium stores computer-readable program instructions (i.e., computer programs) for executing the above-mentioned component separation method, which can solve the technical problem of how to improve the working efficiency of a liquid chromatograph. Compared with the prior art, the beneficial effects of the computer-readable storage medium provided by the present application are the same as those of the component separation method provided by the above embodiments, and will not be elaborated here.

[0156] The present application also provides a computer program product, including a computer program, and when the computer program is executed by a processor, it realizes the steps of the component separation method as described above.

[0157] The computer program product provided by the present application can solve the technical problem of how to improve the working efficiency of a liquid chromatograph. Compared with the prior art, the beneficial effects of the computer program product provided by the present application are the same as those of the component separation method provided by the above embodiments, and will not be elaborated here.

[0158] The above are only some embodiments of the present application, and thus do not limit the patent scope of the present application. Any equivalent structural transformation made under the technical concept of the present application by using the content of the specification and drawings of the present application, or any direct / indirect application in other related technical fields, is included in the patent protection scope of the present application.

Claims

1. A liquid chromatograph, characterized in that, The liquid chromatograph includes: A solvent and flow path module, which includes a metering pump and at least one mobile phase reservoir. The metering pump is connected to the output port of the mobile phase reservoir. A sample separation module, which includes an injector and a chromatographic column. One end of the injector is connected to the output port of the metering pump, and the other end of the injector is connected to the input port of the chromatographic column. A component detection module, which includes a detector. The input port of the detector is connected to the output port of the chromatographic column. A component collection module, which includes a plurality of intermediate collection bottles and at least one target collection bottle. The intermediate collection bottles are connected to the output port of the detector and the target collection bottle. The intermediate collection bottles are used to subpackage the components output from the output port of the detector, and the target collection bottle is used to combine the components collected by the intermediate collection bottles. A main control module, which is communicatively connected to the solvent and flow path module, the sample separation module, the component detection module, and the component collection module. The main control module at least includes a parameter prediction model for outputting separation parameters based on the molecular structure of the sample to be separated. The main control module is also used to control the solvent and flow path module to output the mobile phase according to the separation parameters, and control the sample separation module, the component detection module, and the component collection module to achieve component separation.

2. The liquid chromatograph according to claim 1, characterized in that, The liquid chromatograph further includes: A mobile phase detection sensor, whose input port is connected to the output port of the mobile phase reservoir, and whose output port is connected to the input end of the metering pump. The main control module is also communicatively connected to the mobile phase detection sensor. The main control module is also used to output a stop separation instruction to the metering pump when it detects that the data collected by the mobile phase detection sensor indicates that the mobile phase reservoir is in an empty bottle state.

3. The liquid chromatograph according to claim 1, wherein The liquid chromatograph further includes: A first air pump, which is communicatively connected to the main control module. A first one-way valve, whose input port is connected to the output port of the first air pump, and whose output port is connected to the pipeline between the detector and the chromatographic column. The main control module is also used to control the first air pump to output air to avoid residual liquid in the pipeline between the detector and the chromatographic column.

4. The liquid chromatography instrument according to claim 3, characterized in that, The liquid chromatograph further includes: A second one-way valve, whose input port is connected to the output port of the chromatographic column. A pressure sensor, one end of which is connected to the output port of the second one-way valve, and the other end of which is connected to the input port of the detector. The main control module is also communicatively connected to the pressure sensor. The main control module is also used to detect the pipeline blockage state according to the pressure collected by the pressure sensor.

5. The liquid chromatograph according to claim 1, characterized in that, The liquid chromatograph further includes: An automatic cleaning module, the automatic cleaning module includes a diaphragm pump, a switching valve, a spray liquid storage bottle and a waste liquid storage bottle. One end of the diaphragm pump is connected to the output port of the spray liquid storage bottle, the other end of the diaphragm pump is connected to one end of the switching valve, the other end of the switching valve is connected to one end of the intermediate collection bottle, and the other end of the intermediate collection bottle is connected to the waste liquid storage bottle; The main control module is also communicatively connected to the switching valve, and the main control module is also used to transmit an opening instruction or a closing instruction to the switching valve to clean the intermediate collection bottle with the spray liquid in the spray liquid storage bottle and collect the spray liquid after cleaning the collection bottle through the waste liquid storage bottle.

6. The liquid chromatography instrument according to claim 1, characterized in that, The liquid chromatograph further includes: A second air pump, the second air pump is communicatively connected to the main control module; A third one-way valve, the input port of the third one-way valve is connected to the output port of the second air pump; A first solenoid valve, the input end of the first solenoid valve is connected to the output port of the third one-way valve and the output port of the metering pump. The first output port of the first solenoid valve is connected to the input port of the injector, and the second output port of the first solenoid valve is connected to the output port of the chromatographic column; The main control module is also used to control the second air pump to output air to clean the chromatographic column and discharge the residual liquid.

7. A component separation method, characterized in that, The component separation method is applied to the main control module of the liquid chromatograph according to any one of claims 1 to 6; The component separation method includes: Obtaining the separation parameters of the sample to be separated through the parameter prediction model in the main control module, and controlling the solvent and flow path module to output the mobile phase through the separation parameters; Controlling the sample separation module to separate the sample to be separated containing the mobile phase, and controlling the component detection module to detect the liquid separated by the sample separation module to obtain detection data; Controlling the intermediate collection bottle in the component collection module to collect the liquid, and determining the intermediate collection bottle numbers corresponding to each component based on the detection data; For each component, using the intermediate collection bottle corresponding to the intermediate collection bottle number of the component as the collection bottle to be emptied, and transferring the liquid in the collection bottle to be emptied to the target collection bottle preset for the component to obtain each of the separated components.

8. The component separation method according to claim 7, characterized in that, The detection data includes an elution curve; The step of determining the intermediate collection bottle numbers corresponding to each component based on the detection data includes: Determining the peak times of each component in the sample to be separated based on the elution curve; For each component, determining the start collection time and the end collection time corresponding to the component according to the peak time corresponding to the component and the elution curve; For each component, determining the intermediate collection bottle number corresponding to the component according to the start collection time and the end collection time corresponding to the component to obtain the intermediate collection bottle numbers corresponding to each component.

9. A storage medium, characterized in that, The storage medium is a computer-readable storage medium, and a computer program is stored on the storage medium. When the computer program is executed by a processor, the steps of the component separation method according to any one of claims 6 to 8 are implemented.

10. A computer program product, characterized in that, The computer program product includes a computer program which, when executed by a processor, implements the steps of the component separation method according to any one of claims 6 to 8.

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