The invention provides a valve ball loading device of an automatic ball valve assembling machine, belonging to the technical field of machinery, and solving the problems that the traditional ball valve is assembled by the hands, the assembling efficiency is low, the labor cost is high and the like. The automatic ball valve assembling machine comprises a worktable, wherein the worktable is fixedly connected with a die holder, the die holder moves together with the worktable, and the valve ball loading device is arranged on the side edge of the worktable and comprises a support, a vibration tray, a sliding-table air cylinder and a positioning bar, wherein the upper part of the support is fixedly connected with a horizontal guide rail, the horizontal guide rail is movably connected with a mechanical hand, the sliding-table air cylinder is positioned in the middle of the support, the positioning bar is fixed on a sliding table of the sliding-table air cylinder, one end of the positioning bar is provided with a positioning groove, the positioning groove is formed in a discharging port of the vibration tray, and a valve ball in the positioning groove can be moved by the mechanical hand into a valve body on the die holder. The valve ball loading device has the advantages of high automation degree, high ball valve assembling efficiency, and the like.