Thrombus analysis device

Abstract

The invention provides a thrombus analysis device, and belongs to the technical field of thrombus analysis. The thrombus analysis device comprises a detection cup, a magnetic bead, a coil driving assembly, a light source and a camera module, the detection cup is used for accommodating a to-be-detected sample; a reflecting coating is arranged on the surface of the magnetic bead, and the magnetic bead is placed in a detection cup; the coil driving assembly is located outside the detection cup and used for driving the magnetic beads to swing back and forth in the detection cup; the light source is arranged towards the detection cup and is used for emitting a light source into the detection cup so as to illuminate the magnetic beads in the detection cup; the camera module is arranged towards the detection cup, and the camera module is used for capturing the moving track of the magnetic bead in the detection cup and the image data of the to-be-detected sample in the detection cup. By arranging the camera module, the light source and the reflective coating on the surface of the magnetic bead, the motion trail of the magnetic bead is captured, the motion trail of the magnetic bead in a three-dimensional space in the detection cup is captured and recorded, the data precision is improved, and the error of a detection result is reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of thrombus analysis, and particularly relates to a thrombus analysis device. Background Art

[0002] Thrombus analysis refers to detecting and analyzing the formation process, components, structure, function, etc. of thrombi to understand the nature, occurrence and development mechanism of thrombi and their impact on the body, which is of great significance for the diagnosis, treatment and prevention of thrombus-related diseases.

[0003] The formation of thrombi is closely related to blood coagulation. If the coagulation time is too short, it may indicate that the blood is in a hypercoagulable state and is prone to form thrombi; if the coagulation time is too long, it may indicate coagulation dysfunction and is prone to bleeding. Studying the blood coagulation time provides a basis for the diagnosis and treatment of thrombus-related diseases.

[0004] In the prior art, the magnetic bead method is used to detect the blood coagulation time. In the related art, the running state of magnetic beads is usually detected by the infrared light emission and reception detection method. The infrared light signal is strong and does not require multi-stage amplification processing of the received signal, which can simplify the circuit design of the magnetic bead detection channel and reduce the cost. However, the data obtained by this detection method is single and the accuracy is low, resulting in a large error in the finally obtained detection result. Summary of the Invention

[0005] The purpose of the present application is to provide a thrombus analysis device to solve the above technical problems existing in the prior art.

[0006] The present application is implemented as follows: The embodiment of the present application provides a thrombus analysis device, including a detection cup, magnetic beads, a coil driving component, a light source and a camera module; the detection cup is used to hold the sample to be tested; a reflection coating is provided on the surface of the magnetic beads, and the magnetic beads are used to be placed in the detection cup; the coil driving component is located outside the detection cup and is used to drive the magnetic beads to swing back and forth in the detection cup; the light source is arranged facing the detection cup, and the light source is used to emit light into the detection cup to illuminate the magnetic beads located in the detection cup; the camera module is arranged facing the detection cup, and the camera module is used to capture the moving trajectory of the magnetic beads in the detection cup and the image data of the sample to be tested in the detection cup.

[0007] The technical solution adopted by the present invention can achieve the following beneficial effects: In this application, by providing a reflective coating on the surface of the magnetic beads and using the reflective coating in combination with a light source, the visual effect of the magnetic beads is enhanced, facilitating the capture of the position information of the magnetic beads by the camera module to capture the movement trajectory of the magnetic beads, achieving the capture and recording of the movement trajectory of the magnetic beads in the three-dimensional space within the detection cup, improving the data accuracy, and reducing the error of the detection result. Moreover, the cooperation between the light source and the reflective coating can also increase the detection sensitivity and anti-interference ability of the magnetic beads, reducing the interference of turbid samples such as hemolysis and lipemia on the detection result.

[0008] Furthermore, while irradiating the magnetic beads, the light source can also illuminate the sample to be tested, providing light for the sample to be tested, facilitating the acquisition of image data of the sample to be tested by the camera module, and providing data support for subsequent research on blood coagulation. Additionally, the light source in combination with the light reflected by the magnetic beads can further illuminate the sample to be tested around the magnetic beads, thus making it even easier for the camera module to obtain clearer image data of the sample to be tested. BRIEF DESCRIPTION OF THE DRAWINGS

[0009] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments of the present invention or the description of the prior art. Obviously, the following described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0010] Figure 1 is the overall structural schematic diagram of the thrombus analysis device provided by some embodiments of the present application Figure 1 ; Figure 2 is the overall structural schematic diagram of the thrombus analysis device provided by some embodiments of the present application Figure 2 ; Figure 3 is the overall structural schematic diagram of the thrombus analysis device provided by some embodiments of the present application Figure 3 ; Figure 4 is the overall structural schematic diagram of the thrombus analysis device provided by some embodiments of the present application Figure 4 ; Figure 5 is the structural schematic diagram of the magnetic beads provided by some embodiments of the present application; Figure 6 is the structural schematic diagram of the coil driving assembly provided by some embodiments of the present application; Figure 7 is the structural schematic diagram of the camera module provided by some embodiments of the present application; Figure 8 is the external schematic diagram of the thrombus analysis device provided by some embodiments of the present application Figure 1 ; Figure 9External schematic of a thrombus analysis device provided by some embodiments of the present application Figure 2 ; Figure 10 Regarding Figure 9 Detail view of point A of

[0011] In the figure: 100 - detection cup, 200 - magnetic bead, 300 - reflective coating, 310 - identification area, 400 - coil driving assembly, 410 - first electromagnetic driving unit, 420 - second electromagnetic driving unit, 500 - light source, 600 - camera module, 610 - first camera, 620 - second camera, 700 - isolation coating, 800 - mounting body. Detailed implementation manners

[0012] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation manners obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0013] In the related art, the position of the magnetic bead is detected by the method of infrared light emission and reception detection. However, the detection results obtained by this method have large errors. Through research by the inventor, it is found that when the magnetic bead moves, it has multiple moving directions, while the infrared light emission and reception detection method can only detect the movement of the magnetic bead in one direction. Therefore, the obtained detection data is single, with large errors and low accuracy.

[0014] In view of this, the embodiments of the present application provide a thrombus analysis device that uses the camera module 600 to detect the movement trajectory of the magnetic bead 200 in three-dimensional space, improves the detection accuracy of the position of the magnetic bead 200, and thus reduces the error of the finally obtained detection results.

[0015] The analysis device provided by the embodiments of the present application includes a detection cup 100, a magnetic bead 200, a coil driving assembly 400, a light source 500, and a camera module 600, as shown in reference Figures 1 to 4 shown.

[0016] The detection cup 100 is used to hold the sample to be tested, and the sample to be tested is placed in the detection cup 100. The sample to be tested is a blood sample that needs to be detected. The surface of the magnetic bead 200 is provided with a reflective coating 300, and the magnetic bead 200 is used to be placed in the detection cup 100. The coil driving assembly 400 is located outside the detection cup 100 and is used to drive the magnetic bead 200 to swing back and forth in the detection cup 100. The light source 500 is arranged facing the detection cup 100, and the light source 500 is used to emit light into the detection cup 100 to illuminate the magnetic bead 200 located in the detection cup 100. The camera module 600 is arranged facing the detection cup 100 and is used to capture the movement trajectory of the magnetic bead 200 in the detection cup 100 and the image data of the sample to be tested in the detection cup 100.

[0017] The magnetic bead method is a method for measuring blood coagulation function based on the viscosity change during blood coagulation. A blood sample is collected, and appropriate reagents such as blood coagulation activators are selected according to the detection items. Then the blood sample is put into the detection cup 100, and the corresponding blood coagulation activator reagent is added, and the instrument is started to start the detection. The coil driving assembly 400 generates a constant alternating electromagnetic field, so that the magnetic bead 200 in the detection cup 100 maintains an equal-amplitude oscillating motion. As the plasma coagulates, fibrin gradually forms, the viscosity of the plasma increases, and the motion amplitude of the magnetic bead 200 gradually weakens. When the motion amplitude of the magnetic bead 200 decays to a certain extent, it is judged as the coagulation end point of the blood.

[0018] In the embodiment provided by the present application, the light emitted by the light source 500 can pass through the detection cup 100 and irradiate the surface of the magnetic bead 200 in the detection cup 100. The reflective coating 300 on the surface of the magnetic bead 200 is used to reflect the light emitted by the light source 500, thereby improving the visual effect of the magnetic bead 200, making the magnetic bead 200 more prominent in the sample to be tested, and making it easier for the camera module 600 to capture the position of the magnetic bead 200, so as to realize the capture and recording of the motion trajectory of the magnetic bead 200 in the three-dimensional space in the detection cup 100. Compared with the existing method of using infrared light emission and reception for detection, in the embodiment of the present application, the camera module 600 can directly capture the motion trajectory of the magnetic bead 200 in the three-dimensional space, and its data accuracy is higher, and the moving direction of the magnetic bead 200 in the three-dimensional space can be clearly distinguished, thereby avoiding detection errors caused by the inability to detect the movement of the magnetic bead 200 in some directions.

[0019] By cooperating the light source 500 with the reflective coating 300 on the surface of the magnetic bead 200, the interference of turbid samples such as hemolysis and lipemia on the detection results can be reduced, the detection sensitivity and anti-interference ability of the camera module 600 to the magnetic bead 200 can be increased, the situation of identifying the substances in the sample to be tested as the magnetic bead 200 can be avoided, and the situation of the substances in the sample to be tested blocking the magnetic bead 200 and making it impossible to identify the magnetic bead 200 can be avoided.

[0020] The motion trajectory of the magnetic bead 200 is obtained by the imaging module 600. It has a large amount of data samples, high data accuracy, and small errors. When studying the blood coagulation time subsequently, it has a more accurate data basis, which is more convenient for the development of research and the obtaining of research results.

[0021] Moreover, while the light source 500 irradiates the magnetic bead 200, it can also illuminate the sample to be tested at the same time, providing light for the sample to be tested, so as to facilitate the imaging module 600 to obtain the image data of the sample to be tested. As the magnetic bead 200 swings in the sample to be tested, a series of image data of the sample to be tested during the coagulation change process can be obtained. These image data are correlated with the local blood coagulation process. The image data and the large model can be used to fuse and correct the blood coagulation detection results, thereby further improving the accuracy of the detection results. The whole process dynamics of blood coagulation initiation, fibrin formation, and platelet participation can also be analyzed.

[0022] The reflective coating 300 on the surface of the magnetic bead 200 can reflect light, which can further illuminate the sample to be tested around the magnetic bead 200, thus making it more convenient for the imaging module 600 to obtain clearer image data of the sample to be tested.

[0023] During the implementation of the embodiments provided in this application, the imaging module 600 is used to obtain the image data of the magnetic bead 200 during the movement process. By combining a high-resolution camera and an accurate three-dimensional reconstruction algorithm, high-precision measurement of the movement speed and position of the small ball is achieved. In some preferred embodiments, the rotation of the magnetic bead 200 can also be identified by restricting the structure of the reflective coating 300.

[0024] Exemplarily, the reflective coating 300 includes at least one identification area 310. Refer to Figure 5 As shown, the identification area 310 is annular and only covers a part of the surface of the magnetic bead 200. On the one hand, during the movement of the magnetic bead 200, at least part of the identification area 310 can be exposed in the shooting direction of the imaging module 600, so as to facilitate the imaging module 600 to obtain the image data of the magnetic bead 200. On the other hand, during the rotation of the magnetic bead 200, the annular identification area 310 will be in different forms, so as to judge the movement state of the magnetic bead 200 by judging the form and position of the identification area 310.

[0025] Driven by the coil driving assembly 400, the magnetic bead 200 swings back and forth in the test cup 100. When the magnetic bead 200 rotates while swinging, it will affect the movement speed of the magnetic bead 200, thereby affecting the attenuation of the movement amplitude of the magnetic bead 200 and affecting the final detection result.

[0026] In the embodiments of the present application, an annular identification area 310 is provided on the surface of the magnetic bead 200 to identify the rotational movement of the magnetic bead 200 and obtain the rotational data of the magnetic bead 200. When calculating the measurement result, the measurement result can be corrected based on the rotational data of the magnetic bead 200, thereby reducing or even avoiding the error caused by the rotation of the magnetic bead 200 to the measurement result.

[0027] In some preferred embodiments, as shown in Figure 5 FIG. 5, there are multiple identification areas 310. The multiple identification areas 310 are coaxially arranged and are arranged at intervals in sequence. The increase in the number of the identification areas 310 makes it easier for the camera module 600 to identify the magnetic bead 200 and obtain the position information of the magnetic bead 200. The coaxial arrangement of the multiple identification areas 310 enables the synchronous rotation of the multiple identification areas 310, reducing the difficulty for the camera module 600 to identify the identification areas 310. At the same time, the multiple identification areas 310 increase the total area of the reflective coating 300, further improving the visual effect of the magnetic bead 200 in the sample to be measured and facilitating the identification of the magnetic bead 200 in the sample to be measured.

[0028] Further preferably, along the arrangement direction of the multiple identification areas 310, the width of the identification area 310 gradually increases or gradually decreases. The width of each identification area 310 is different and gradually changes along its arrangement direction. When the magnetic bead 200 rotates, by observing the change trend of the width of the identification area 310, the rolling direction of the magnetic bead 200 can be distinguished, providing more accurate data support for the correction of the final detection result to further reduce the error of the detection result. The multiple identification areas 310 are preferably evenly distributed on the surface of the magnetic bead 200.

[0029] Compared with the prior art where only an infrared detection device can be used to detect whether the magnetic bead 200 passes through a certain fixed detection point, the analysis device provided in the embodiments of the present application can not only determine the running position of the magnetic bead 200, but also determine the running state of the magnetic bead 200, and correct the detection result based on the self-movement data of the magnetic bead 200, further improving the accuracy of the detection result.

[0030] In some other embodiments, different identification areas 310 can be set to different colors, and the rotation direction of the magnetic bead 200 can be identified by the trend of color change.

[0031] In some embodiments provided by the present application, an isolation coating 700 is further provided on the surface of the reflective coating 300, and the isolation coating 700 can allow light to pass through. The isolation coating 700 is used to isolate the reflective coating 300 from the external environment corresponding to the magnetic bead 200, that is, the isolation coating 700 separates the reflective coating 300 from the sample to be measured, avoiding the influence of the reflective coating 300 on the reaction of the sample to be measured.

[0032] The isolation coating 700 can be optionally transparent and is coated on the outermost layer of the magnetic beads 200 to isolate the reflective coating 300. At the same time, it does not affect the transmission of light. In some embodiments, the isolation coating 700 only covers the surface of the reflective coating 300. In other embodiments, the isolation coating 700 covers all surfaces of the magnetic beads 200. The light emitted by the light source 500 needs to pass through the test cup 100 and the isolation coating 700 to irradiate the surface of the reflective coating 300. The test cup 100 also needs to be made of a transparent material.

[0033] In addition, in the embodiments provided in the present application, the structure of the test cup 100 is not improved, and an existing transparent test cup 100 can be directly selected for use, thereby reducing the transformation cost of the analysis device.

[0034] In some preferred embodiments, referring to Figure 1 and Figure 2 as shown, the light source 500 is in a ring structure, and the light source 500 is sleeved outside the test cup 100. The ring-shaped light source 500 can provide uniform light, avoid detection dead angles, and thus improve the accuracy of the detection results.

[0035] The camera module 600 provided in the embodiments of the present application can acquire data such as the movement trajectory of the magnetic beads 200 and the image information of the sample to be tested. The camera module 600 includes a first camera 610. The viewing direction of the first camera 610 intersects with the plane corresponding to the cross-section of the test cup 100. Referring to Figure 3 as shown, and in the axial direction of the test cup 100, the first camera 610 is arranged closer to the cup mouth of the test cup 100 relative to the coil driving assembly 400.

[0036] The viewing direction of the first camera 610 refers to the direction perpendicular to its viewing surface. Referring to Figure 3 the dotted line direction shown. The cross-section of the test cup 100 refers to the section perpendicular to its axis. There is an angle between the plane corresponding to this section and the viewing direction of the first camera 610. The first camera 610 is located above the coil driving assembly 400, and the first camera 610 acquires the image information of the magnetic beads 200 from above.

[0037] The camera module 600 further includes a second camera 620. Referring to Figure 4 as shown, the viewing direction of the second camera 620 is arranged parallel to the plane corresponding to the cross-section of the test cup 100. The camera module 600 includes the first camera 610 and the second camera 620. Referring to Figure 7As shown, the second camera 620 and the first camera 610 cooperate with each other. Using the principle of stereoscopic vision and the three-dimensional reconstruction algorithm, based on the two-dimensional coordinates of the magnetic bead 200 in the images of different cameras and the parameters of the cameras, the coordinates of the magnetic bead 200 in the three-dimensional space are calculated to obtain the position information of the magnetic bead 200. Further, the position of the second camera 620 is at the same height as the position of the coil driving assembly 400. The second camera 620 is used to judge the movement of the magnetic bead 200 in the direction of gravity, which is more convenient for subsequent correction of the detection results.

[0038] Both the first camera 610 and the second camera 620 can be connected to a computer, and the operation of the camera module 600 is controlled by the computer.

[0039] The coil driving assembly 400 includes a first electromagnetic driving unit 410 and a second electromagnetic driving unit 420. Refer to Figure 6 As shown, the first electromagnetic driving unit 410 and the second electromagnetic driving unit 420 are respectively located on both sides of the test cup 100, and are used to apply an alternating magnetic field to the magnetic bead 200 in the test cup 100 to drive the magnetic bead 200 to move.

[0040] The first electromagnetic driving unit 410 and the second electromagnetic driving unit 420 can be connected to a computer, and the supply of alternating current is controlled by the computer. Both the first electromagnetic driving unit 410 and the second electromagnetic driving unit 420 include coil windings. The coil winding is the core component, usually a multi-turn coil wound by a wire and used in cooperation with a magnetic core.

[0041] The thrombus analysis device further includes a mounting body 800. The mounting body 800 has a mounting portion, and the test cup 100 is detachably mounted to the mounting portion. The coil driving assembly 400, the light source 500, and the camera module 600 are all fixedly mounted on the mounting body 800. The test cup 100 is detachable, which is convenient for replacing the test cup 100 to detect different samples, and the other parts are fixedly arranged.

[0042] The mounting body 800 refers to a structure that can support the other components. The mounting body 800 is a general term. In some embodiments, refer to Figures 1 to 4 As shown, the mounting body 800 includes at least two parts. One part is provided with a through hole, and the test cup 100 passes through the through hole. The camera module 600, the light source 500, and the coil driving assembly 400 are all fixed to this part. The other part is provided with a mounting groove. After the test cup 100 passes through the through hole, the bottom of the cup is placed in the mounting groove, and the two parts cooperate to limit the position of the test cup 100.

[0043] In some other embodiments, the overall volume of the thrombus analysis device is relatively large, and the overall size of the mounting body 800 is relatively large. The thrombus analysis device can also be integrated with other devices on the same equipment. Refer toFigure 8 and Figure 9 As shown, the installation main body 800 includes the housing frame of the integrated device. Refer to Figure 10 As shown, the integrated device reserves a window to facilitate the installation and removal of the test cup 100.

[0044] In some preferred embodiments, in the axial direction of the test cup 100, the light source 500 is arranged relatively close to the cup mouth of the test cup 100 with respect to the coil driving assembly 400. The axial direction of the test cup 100 is generally parallel to the direction of gravity. The light source 500 is located above the coil driving assembly 400 to prevent the light source 500 from affecting the generation of the alternating magnetic field of the coil driving assembly 400. At the same time, the magnetic beads 200 move within the range of the alternating magnetic field generated by the coil driving assembly 400. The light source 500 located above can provide sufficient light for the magnetic beads 200. The light source 500 is preferably also located above the camera module 600 or at least flush with the camera module 600. When the camera module 600 acquires the image information of the magnetic beads 200 and the sample to be tested, the situation of acquiring image information against the light can be avoided, improving the accuracy of the data.

[0045] It should be noted that in this article, the terms "include", "comprise" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "including one..." does not exclude the existence of additional identical elements in the process, method, article or device including that element.

[0046] The above is only the specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention can easily think of changes or substitutions, which should all be covered within the protection scope of the present invention.

Claims

1. A thrombus analysis device, characterized in that, Comprising: A detection cup (100) for containing a sample to be tested; Magnetic beads (200) with a reflective coating (300) provided on the surface thereof, and the magnetic beads (200) are for being placed in the detection cup (100); A coil driving assembly (400) located outside the detection cup (100) for driving the magnetic beads (200) to swing back and forth in the detection cup (100); A light source (500) arranged facing the detection cup (100), and the light source (500) is for emitting a light source (500) into the detection cup (100) to illuminate the magnetic beads (200) located in the detection cup (100); An imaging module (600) arranged facing the detection cup (100), and the imaging module (600) is for capturing the moving trajectory of the magnetic beads (200) in the detection cup (100) and the image data of the sample to be tested in the detection cup (100).

2. The thrombus analysis device according to claim 1, wherein, The reflective coating (300) includes at least one identification area (310), and the identification area (310) is annular.

3. The thrombus analysis device according to claim 2, wherein There are multiple identification areas (310), and the multiple identification areas (310) are coaxially arranged and are arranged at intervals in sequence; Along the arrangement direction of the multiple identification areas (310), the width of the identification area (310) gradually increases or gradually decreases.

4. The thrombus analysis device according to claim 1, characterized in that, An isolation coating (700) is further provided on the surface of the reflective coating (300), and the isolation coating (700) can allow light to pass through, and the isolation coating (700) is for isolating the external environment corresponding to the reflective coating (300) and the magnetic beads (200).

5. The thrombus analysis device according to claim 1, characterized in that, The light source (500) is of an annular structure, and the light source (500) is sleeved outside the detection cup (100).

6. A thrombus analysis device according to claim 1, characterized in that, The imaging module (600) includes a first camera (610), and the viewing direction of the first camera (610) intersects with the plane corresponding to the cross-section of the detection cup (100), and in the axial direction of the detection cup (100), the first camera (610) is closer to the cup mouth of the detection cup (100) than the coil driving assembly (400).

7. The thrombus analysis device according to claim 6, characterized in that The imaging module (600) further includes a second camera (620), and the viewing direction of the second camera (620) is parallel to the plane corresponding to the cross-section of the detection cup (100).

8. A thrombus analysis device according to claim 1, characterized in that, The coil driving assembly (400) includes a first electromagnetic driving unit (410) and a second electromagnetic driving unit (420), and the first electromagnetic driving unit (410) and the second electromagnetic driving unit (420) are respectively located on both sides of the detection cup (100) and are for applying an alternating magnetic field to the magnetic beads (200) in the detection cup (100).

9. A thrombus analysis device according to claim 1, characterized in that, The thrombus analysis device further includes a mounting body (800), the mounting body (800) has a mounting portion, the detection cup (100) is detachably mounted to the mounting portion, and the coil driving assembly (400), the light source (500), and the imaging module (600) are all fixedly mounted on the mounting body (800).

10. A thrombus analysis device according to claim 9, characterized in that, In the axial direction of the detection cup (100), the light source (500) is disposed closer to the cup mouth of the detection cup (100) than the coil driving assembly (400).

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